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EVALUATION OF THE WOUND HEALING EFFECT OF THE CARBONIZED SEED POD OF PENTACLETHRA MACROPHYLLA BENTH IN DIABETIC FOOT ULCER by Johnson–Ajinwo in  Journal of Clinical & Medical Case Reports, Images (JCMCRI) ISSN: 3064-8025

Introduction

Diabetes, a global disease estimated to have a global burden of about 693 million individuals by 2045 [1] is a metabolic disorder caused by abnormalities in insulin secretion, action, or both. Diabetic patients are more likely to get wounds, and these wounds frequently require more time to heal because of a variety of issues including immunological dysfunction, vascular disease, and neuropathy [2]. Diabetes-related wounds pose a substantial risk to one's health because they might result in amputation, infections, or even death [3]. The pathophysiology of DFU is multifaceted and is an interlink between the neuropathic, vascular, and immune systems [4]. Because neuropathy results in decreased sensation, a diabetic patient may develop a cut on the foot and would not notice until an ulcer is formed. Also, diabetic nerves are poorly perfused, and the wound-healing process is slower than usual due to the slow migration of inflammatory mediators to the injury site [5]. Diabetic Foot Ulcers (DFUs) are significant comorbidities of diabetes mellitus that demand medical attention and have the potential to be fatal. DFU affects 19–34 % of diabetics at some point in their lives, and 9.1–26.1 million people are estimated to get DFU annually by the International Diabetes Federation [6]. In the UK, population-based cohort research indicated that 5% of the study population developed foot ulcers, with 5% of those developing new ulcers dying within the first 12 months of ulcer development and a death rate of 42.2% for those with foot ulcers [7].

Additionally, patients with DFU experience higher morbidity, a greater burden of healthcare contacts, a lower quality of life related to their health, and a worse psychosocial adjustment [8]. The current management of DFU includes Offloading, surgical debridement, use of topical dressings, and achieving glycemic control [9].

African oil bean tree or Pentaclethra macrophylla, is a plant with a long history of traditional use for wound healing. Alkaloids, flavonoids, and saponins are among the bioactive substances found in Pentaclethra macrophylla seeds [10]. Research has demonstrated that these compounds have antibacterial, anti-inflammatory, and antioxidant properties [11, 12]. Furthermore, the leaves of this plant have been shown to reduce blood glucose levels. Hence, these properties suggest that Pentaclethra macrophylla might be useful as a DFU treatment [13].

MATERIALS AND METHODS

2.1 COLLECTION OF PLANT MATERIAL The seed pod of the Pentaclethra macrophylla plant was collected from a bio-reserve in Ethiope West LGA of Delta State, Nigeria on February 2023. Soon after collection, the seed pods were cleaned and shade-dried. The plant was identified, confirmed, and authenticated by Dr. Suleiman, a Taxonomist in the Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences University of Port Harcourt Rivers State, Nigeria. (Voucher No: UPHF0598). A voucher specimen of the plant was deposited in the Departmental herbarium for future reference. After drying, they were crushed to a powder and stored in an airtight plastic container for further use.

2.2 EXTRACTION The crushed seed pod powder was weighed and then carbonized at 550 - 600°C in a Muffle furnace. The carbonized sample was properly crushed into fine particles and sieved through a 125um sieve.

2.3 QUALITY CONTROL PARAMETERS Coarse powder of the seed pod of Pentaclethra macrophylla was used to perform quality control test which include the determination of acid-insoluble ash and water-soluble ash.

2.3.1 Acid insoluble ash (AIA): 40ml of concentrated HCl was diluted with distilled water to make the volume 100ml and was stored in an amber glass bottle. About 2gm of the powdered drug was weighed accurately into a tared silica crucible and incinerated at 450°C in a muffle furnace until free from carbon. 25ml of the 40% HCl solution was poured into the crucible with the ash and boiled for 5 minutes. After which, it was filtered with an ash-less filter paper, and the crucible was properly rinsed with additional hot water to ensure that no residue of HCl was left in the filter paper. The crucible and the lid were placed inside the hot air oven at 130°C for about 20 minutes, then cooled in the desiccator and weighed blank. The filter paper was transferred into the prepared crucible and incinerated at 450°C in a muffle furnace until free from carbon. The crucible containing the acid-insoluble ash was cooled and weighed. The percentage of acid-insoluble ash was calculated as follows with reference to the air-dried substance. The procedure for the acid-insoluble and water-soluble ash was carried out according to the procedure of the Association of Official Analytical Chemists [14]. Results were calculated as,

Acid insoluble ash = Wf - Wc/ Ws Where, Wf = Crucible + Ash weight Wc = Crucible weight Ws = Sample weight 2.3.2 Water soluble ash: Same procedure as in acid insoluble ash. Where 25ml of 40% HCl solution was poured into the crucible with the ash and boiled for 5 minutes, 25ml of distilled water was used instead.

 2.4 FORMULATION OF CREAM The formula for cream formation is as follows: Plant Extract:                                      4%, 2.5%, 1% Emulsifying ointment:                        30% Shear butter                                         20% Purified water to                                 100% A 50 g preparation was made.

Preparation of emulsifying ointment: Formula got from Pharmaceutical codex 1979 Rx Emulsifying wax 300 g White soft paraffin 500 g Liquid paraffin 200 g The formula was reduced by 50%

Procedure for Formulation of Emulsifying Ointment The three ingredients were accurately weighed and/or measured and transferred into the glass container. Subsequently, they were melted over a water bath and stirred until cooled to form the emulsifying ointment.

Method of cream formation:

15 g of Emulsifying ointment and 10 g of shear butter were combined in a beaker and gently heated over a water bath until melted. 23 ml of purified water was measured into another beaker and placed over the water bath. The thermometer was used to regulate their temperature to 60 °C. The mixture was transferred into a porcelain mortar and stirred. A 2g of the carbonized extract was incorporated into this to give a 4% cream.

This base was then transferred into a wide-mouthed plastic jar, capped and labeled appropriately. For the 2.5% and 1% cream formulation, 1.25g and 0.5g of the plant powdered extract were added respectively in place of 2g. 23.75ml and 24.5ml of purified water were used respectively in place of 23ml.

The base was prepared following the method without incorporating the carbonized plant extract.

2.5       PHARMACOLOGICAL STUDY 2.5.1    ANIMALS AND MANAGEMENT: Thirty-six (36) Wistar albino rats male and female weighing between 150 g - 200 g were obtained from the Department of Experimental Pharmacology & Toxicology Animal House, University of Port Harcourt. They were sorted, and housed in standard cages with housing conditions consisting of 12 hours of light and dark cycles. They were fed with standard rat pellets and water ad libitum. The rats were maintained at room temperature and allowed to acclimatize to the new environment for two weeks.

2.5.2 DRUG: Commercially available Dermazin cream and Glibenclamide were used as the control drug. The Dermazin was applied topically over the wound area while the glibenclamide was administered orally.

 2.5.3 Induction of Diabetes: Diabetes was induced in the rats by using Alloxan Monohydrate (Sigma-Aldrich), a compound that has preferential toxicity towards pancreatic beta cells. Diabetic conditions were induced by a single intraperitoneal injection of Alloxan at a concentration of 150 mg/kg body weight in overnight fasted rats [15]. After three days of induction, blood samples were collected from the tail and measured using an Accu-Check glucometer. Animals with fasting blood glucose levels from 10 mMol/L and above were considered diabetic.

2.5.4 Evaluation of Wound Healing Effect of Pentaclethra Macrophylla on Excision Wound: On the fourth day after the induction of the Alloxan, the diabetic rats were randomly classified into 9 groups. Group 1 (Diabetic wounded and treated with cream base)

Group 2 (Diabetic wounded and treated with Dermazin cream + Glibenclamide) Group 3 (Diabetic wounded and treated with Dermazin cream) Group 4 (Diabetic wounded and untreated) Group 5 (Non-diabetic wounded and untreated) Group 6 (Diabetic wounded and treated with 4% cream of carbonized plant sample) Group 7 (Diabetic wounded and treated with 2.5% cream of carbonized plant sample) Group 8 (Diabetic wounded and treated with 1% cream of carbonized plant sample) Group 9 (Diabetic wounded and treated with 2.5% cream of carbonized plant sample + Glibenclamide)

Induction of Wound On the wounding day, the rats were anaesthetized with diethyl ether. prior to the creation of the wounds. The dorsal fur of the animals was shaved with an electric clipper and the area for excision was marked. Following proper aseptic techniques, a 1.5cm wound was created on the marked area. The wound was left for some days until it turned into an ulcer. The cream was applied gently to cover the ulcerated area once daily for 21 days. The wound area and rate of wound contraction were monitored [16].

 2.6       Estimation of Parameters Measurement of Wound Contraction: The wound healing progression was evaluated by the periodic measurement of the contraction of the excision site [17].

Wound contraction was monitored by tracing the outline of the wound on a tracing sheet and then using a graph sheet to calculate the area of the wound size. All animals in each group were monitored until complete healing of wounds occurred and the mean daily wound healing for all groups was calculated.

Wound healing was calculated by : 2.7 Histological Analysis of Tissues Following the conclusion of the experiment, the animals were sacrificed according to ethical guidelines and had histological tissue examination according to the procedure by Slaoui, et al [18].

 2.8       Statistical Analysis Tests were carried out in quadruplets and their values were expressed as mean ± standard error of mean (SEM). The One-way analysis of variance (ANOVA) was used to determine the significant differences amongst all groups against control and the P value < 0.05 was considered as significant. All statistical analysis was performed using Graph Pad Prism version 8.0 software.

RESULTS

3.1 Result of Quality Control Analysis Physicochemical analysis was carried out on the pulverized seed pod of Pentaclethra macrophylla Benth. The acid-insoluble ash (AIA), and water-soluble ash were determined as represented in Table 1. The result contained in Table 1 shows that the extract is free of inorganic residue as only 1% of AIA is present in the carbonized sample; which is negligible.

3.2 Pharmacological Studies Results Following wound induction, images were taken across the various test groups to show the rate of wound healing. These images are presented below:

3.3: Fasting Blood Glucose Results Alloxan was used to induce diabetes in the test animals. Their blood glucose levels were measured before and after induction. These values are represented in Table 2.

3.4 RESULTS OF HISTOLOGY The results of the histological investigations on the wound healing activities of the various test samples and the control groups are presented in Figure 4 – 12 below.

Table 1: Showing physicochemical parameters of the seed pod of Pentaclethra macrophylla Benth.

Figure 1: Wound contraction of different test groups on Days 1, 7 and 14.

Table 2: Table showing the blood sugar readings of the various groups, before induction, after induction, and on Days 7 and 14.

Table 3: Showing the mean and standard error of mean, of the wound contraction in centimeters (cm) on Days 7, 14 and 21.

Figure 2: Chart showing wound contraction in centimeters (cm) for control (Based treated, dermazin treated, diabetic and treated with dermazin plus drug, diabetic and untreated, non-diabetic and untreated, and test group (1% carbonized plant sample, 2.5% carbonized plant sample, 4% carbonized plant sample, 2.5% carbonized plant sample plus drug).

Figure 3: Chart of wound contraction in percentage (%) for control group (Based treated, dermazin treated, diabetic and treated with dermazin plus drug, diabetic and untreated, non-diabetic and untreated and test group (1% carbonized plant sample, 2.5% carbonized plant sample, 4% carbonized plant sample, 2.5% carbonized plant sample plus drug).

Figures 4 & 5

Figures 6 & 7

Figures 8 & 9

Figures 10 & 11

Figures 12: (2.5% CARBONIZED + DRUG) Photomicrograph of rat skin tissue showing distorted epidermal granules, scaring tissue, distinct collagen fibres with distinct nuclei. H & E, X400.

DISCUSSION

Pentaclethra macrophjylla Benth (Fabaceae) is a plant with a long history of traditional use in wound healing [10]. The result of the quality control analysis of the seed pod of Pentaclethra macrophylla is shown in Table 1. The acid-insoluble ash and water-soluble ash values are 1.0% and 2.5% respectively. High acid-insoluble ash values may indicate contamination or poor quality of the sample, affecting its suitability for various applications, while excessive water-soluble ash may suggest the presence of unwanted salts or substances, which can affect the product's quality, taste, or medicinal properties [19]. The low acid insoluble and water soluble ash values suggest that the sample has fewer non-volatile and inorganic contaminants.

DFU is a major complication of diabetes that results in increased hospitalization time, increased health costs, and even death [20].

The wound healing process is a myriad of events. These include homeostasis; the recruitment of platelets and other clotting factors in response to a leaking vessel, inflammation; which involves the invasion of macrophages and neutrophils to clear up any pathogens at the injury site, proliferation; which involves the keratinocytes and matrix deposition to enhance wound closure and remodeling; where collagen is remodeled to finish up the wound healing process [21]. The aim of this study was to compare the different strengths of the carbonized plant extract by evaluating their effectiveness in diabetic wound healing.

From the results obtained, the 2.5% + drug group gave the highest percentage of wound contraction. This was followed by the dermazin + drug group, which is not surprising as the drug; glibenclamide is a sulfonylurea that increases insulin sensitivity of cells. When comparing the Dermazin + drug and the 2.5% + drug groups, they both gave similar percentage wound contraction rates. However, comparing the 1%, 2.5%, and 4% carbonized drug groups, the 2.5% gave a comparable percentage of wound healing as the 4% group. While a comparison of the percentage contraction of the dermazin group to that of the 2.5 and 4% groups, revealed that these two groups demonstrated superior wound healing activities; pointing towards the powerful wound healing effects of the plant. When comparing the results of the 2.5% and 4% groups, it was bserved that the 4% group had a slightly higher wound healing rate from day 0 to 14, which was not statistically significant. But on day 21, the 2.5% treated group showed slightly better results; which were not statistically significant than that of the 4%; suggesting that the 2.5% extract would be the optimal dose for wound healing.

A comparison of the wound healing activities of the extracts and the positive standard, showed that the plant extracts gave a better wound healing profile. Despite the decline in the pursuit of natural products as potential drug leads, encouraging results, like this obtained by the plant extracts of P. macrophylla, provide hope for the use of natural products in disease treatment.

Conclusion

This study demonstrated the wound healing effect of the carbonized seed pod extracts of Pentaclethra macrophylla, with the 2.5% plant extract showing the optimal wound healing activity. This provides a foundation for further research on the plant and its potential as a therapeutic agent for diabetic foot ulcers.

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So, hemp and cannabis are very similar. Legally speaking, it's only hemp if there is 0.3% thc or less. Otherwise its cannabis. Thanks to the 2014 and 2018 farming bills hemp has gained more and more legal status.  Its legal in oil form in most,  if not all us states. At the very least, its no longer expressedly illegal a lot of places. Cannabis has upwards of 500 chemicals and compounds that make it up. These chemicals interact with each other and within your body to create something called the entourage effect. We don't understand a lot of what these compounds do, but we know they work better together than isolated.

Since hemp is lacking thc, it will also lack cannabinoids that come from thc. Delta 9 thc is the most famous one, but delta 8 thc is a thing. Cbn. Metabolites of these. Raw forms of these.  

High quality cannabis will be planted 6+ feet a part. This gives lots of room for shade leaves and flowers to grow. 35% total cannabinoids is about the highest I've seen. High quality hemp will be planted in a similar manner, though 25% is the upper range I've seen for high cbd low thc flowers. These are all female plants to increase resins and decrease chance of seeds.

Now industrial hemp, thats a different beast. I mean its nearly the same as hemp, but its planted 4-6 inches a part. This makes for long stalks with little to no leaves or flowers. The bulk of cannabinoids form in the leaves and flowers. It's also a mix of male and female plants. It's seedy as hell. Industrial hemp has only about  89 cannabinoids compared to nearly 500! The concentration is also significantly lower. Usually around 5%. Most commercial oils dont even use the flowers or leaves for oil making! They produce oils from the sticks stems and seeds! With little cbd present, often harsh chemicals are needed to pull the cbd at all.

But what does this mean? Well think of something thats probably more familiar.  Alcohol by volume. A beer is usually around 6% alcohol, right in line with our industrial hemp figures. Wine and hard liquor. Comes in higher strengths. Port is usually around 15% alcohol. Rum and other liquors are usually  35-40% alcohol.  Then you can go bananas with stuff like 151, or grain alcohol like everclear or graves that can be 195 proof or 95% pure!

It might take 4 or more beers to get  buzz while a single mixed drink will do the same. Or a sip of grain alcohol. Its pretty much the same when you're talking cannabis. Some people may get effects from industrial hemp (the beer in the analogy) but you may need to use a lot of it to see those effects. While a much smaller dose of a high cbd low thc product could do the same for less product (port). Some still might not get desired results. Thats because cbd works best with thc, even if you arent trying to get high. For those people, I'd suggest using a cannabis strain thats high in both thc and cbd. You might need to mix non industrial hemp with cannabis to find what you need. The cannabis is the hard liquor in the analogy.  Concentrated oils like feco, rso, hash, dabs etc. This would be the grain alcohol or 151 equivalent. 

Unfortunately due to the legal status of cannabis, most end up growing their own or purchasing on the black market.  Cbd isn't psychotropic, so unless there's medical needs, most focus on thc to get high. That makes quality cbd sources hard to find in recreational / illegal markets.

Not all extractions are created equal either. Alcohol is a polar solvent. It pulls fat solubles as well as water solubles.  Direct oil infusions will only absorb oil solubles. Cannabinoids are fat soluble, so this isnt a problem. It limits things like chlorophyll,  which is better to get from leafy greens anyway. Co2 on the other hand is a limited extract. You have to blast the plant at multiple pressures to extract multiple cannabinoids. You are essentially making isolates then recombining them with a co2, while an alcohol extract just pulls everything.

In my opinion, the best oils are solvent extracts or direct infusion.

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Zero recurring solvents. Zero pollutants. All those nos indicate that our CBD Isolate is actually some of the purest and most strong isolate on the market. Our company secure our wholesale CBD Segregate to the exact same premium standards that our experts maintain for our very own items. There is one significant variation in between our retail CBD Separate Particle as well as our retail CBD Isolate - cbd wholesale canada.

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that's examined for premium as well as made inexpensive can be actually hard to happen by (making cbd isolate). Our CBD Isolate is actually produced along with the finest farming methods in the greatest quality facility. CBD isolate oil created coming from the hemp vegetation gives an alternative for a lot of that do not prefer the associated high that originates from a Full Sphere distillate or even item that features Delta-9 THC.

Hemp plants contain several cannabinoids, however presently, among the most well-liked ones is actually CBD (Cannabidiol ) - cbd isolate powder bulk. CBD Isolate is actually the point item of an origin method meant to clear away each one of the various other cannabinoids as well as make a pure CBD Oil without any THC (not even map quantities). best cbd bulk.

Having said that, it can be available in greater than one form as well as likewise exists as a powder referred to as "CBD Powder". CBD in its own particle kind is actually generated after CBD oil crystalizes and is at that point eroded in to a particle. While a grain is preferred through some consumers, many still choose to operate along with the oil over the particle.

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If you have a sizable order that you want putting, please call our team directly to acquire Bulk CBD Separate oil costs and an assigned rep to give you along with any kind of details. buy cbd powder. If you're seeking to acquire bulk CBD isolate particle or even oil, at that point register for a wholesale account. pure cbd isolate bulk.

CBD isolate oil is the uncooked material that's instilled in several popular items including: The reason that so many items are infused along with CBD isolate is since CBD isolate has no THC (certainly not even in track quantities) - water soluble nano cbd isolate powder. By means of CBD isolate, you have the ability to receive all the benefits of CBD without the side results of THC or even having to go with exclusive requirements that relate to THC (cbd in bulk).

CBD Segregate oil differs from because, as the title signifies, is actually only CBD however in a separated form as well as without various other substances. CBD distillate on the contrary can be found in 2 various types - you have your typical CBD distillate oil which includes Delta 9 THC as well as Delta 8 Distillate which has - cbd isolate sale.

First, it is very important to comprehend that CBD Separate is a just about pure form of CBD. Hence when trying to find CBD isolate analysis, our company may look for research studies that concentrate on just CBD. low cost canabis oil wholesale. Thus far, CBD isolate research studies have actually shown several benefits, like alleviation coming from anxiety, inflammation, as well as pain relief.

Having said that, even more analysis is still required - buy 1 litre bulk cbd. Other conditions: Provider oil MCT Oil CBD Segregates Marijuana vegetation.

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Why offer CBD oil wholesale? For the last handful of years, CBD oil has actually ended up being a prospering thousand dollar market. People like the several advantages of CBD, as well as it is actually make uses of for a variety of troubles. It's a non-psychoactive substitute to THC, and also it is actually so secure, you can easily also inflict your animals! The greatest main reason to purchase CBD oil as well as CBD isolate wholesale is actually that your consumers desire it.

Compare our CBD isolate wholesale rates along with the rivals - water soluble cbd isolate for sale. Our retail companions recognize the key, and currently therefore do you: at Mile High's Best, our team go over the criteria as well as solution of the industry. From quick and easy internet buying and buying to helpful consumer company, expert advice, and honor succeeding formulas our company are actually a wholesale partner you may depend on.

Our team make use of natural hand-shucked high CBD hemp biomass for CBD isolate and also distillate development. At our removal facility, we only use the finest quality hemp biomass in our CBD isolate and also extract extraction method. The large number of hemp biomass used in manufacturing is actually sourced coming from our personal farm. Hand-shucked biomass is preferred over combined biomass to produce our distillate and also isolate crystal.

Our company has the very best retail rates for high-purity crystalline CBD isolate. IHF LLC CBD isolate crystal is actually sold due to the kg, with more discount rates on larger acquisitions (wholesale cbd makeup). Mass CBD isolate available is actually generated at our sophisticated lab in Denver, CO. Our experts welcome all customers ahead to explore our facility.

If any of these pollutants appeared in hemp utilized for CBD removal, they will likely wind up in a focused kind in the ultimate CBD distillate or isolate. Basically, recognize that you are performing company with as well as where your CBD is originated from. bulk buy cbd products. Brokers only appreciate mark-up as well as there's no lack of $4000 kilos floating around out certainly there fresh off the watercraft from China that exam scorching for heavy metals and also chemicals.

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Our company recognize that certainly not everybody has experienced the benefits of CBD isolate on their own, so it can be actually difficult to understand each of the perks as well as perks it can easily use! As a retail rep, we understand it is vital to be knowledgeable about whatever concerning the product you are selling to deliver your clients along with the greatest details.

CBD isolate are going to not obtain you higher; instead, it is going to relieve stress and also alleviate stress without creating a gloomy or even unfocused headspace. Our CBD isolate is actually additionally fully THC-free. This creates CBD isolates a fantastic choice for consumers who intend to fully steer clear of eating THC in any type of quantity. Our company likewise supply products including trace amounts of THC that aid to motivate the favorable entourage result.

3% or a lot less) to urge the advantages of the entourage result. Total spectrum extracts include all various other valuable terpenes and also cannabinoids normally found in hemp vegetations. Broad scope essences are THC-free. However, they retain the various other cannabinoids as well as helpful terpenes normally current in hemp - what is cbd isolate powder. This enables buyers who wish to stay clear of eating THC a choice that can still help them experience the entourage impact.

Separate is comprised totally of CBD without additional materials. Generally, isolate is the purest kind of CBD, greater than 99% sheer CBD. isolated cbd. Segregate exclusively delivers the impacts of CBD with no various other cannabinoids affecting the adventure. CBD extract is actually different from isolate in that it usually contains much more than 90% CBD.

3% or a lot less). This offers extract a different impact that is actually affected and also modulated due to the impacts of other cannabinoids and also terpenes. cbd crystal. Lots of individuals take CBD isolate for its own wellness-enhancing advantages, however why choose isolate over vast scale or complete scope CBD? Some of the major causes consumers select isolate over various other products is actually to stay clear of eating THC, even when it is actually simply present in trace quantities completely as well as clear scale CBD.

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[market-research-industry]

Global Levulinic acid market to reach a valuation of ~us$ 41.2 MN by 2027: TRANSPARENCY MARKET RESEARCH

Levulinic Acid Market: Introduction

The levulinic acid market was valued at ~US$ 26.2 Mn in 2018 and is anticipated to expand at a CAGR of ~5% from 2019 to 2027. The Asia Pacific led the global agrochemical industry, accounting for approximately 30% share in 2018. Countries in Asia, such as South Korea, China, Japan, and Vietnam, are applying herbicides in large amounts onto short-term as well as perennial crops. Thus, growth of the agrochemical industry in Asia Pacific is driving the levulinic acid market in the region.

Key Drivers of Levulinic Acid Market

According to L'Oréal, a leading manufacturer of cosmetic products, the global cosmetics industry was valued at US$ 532 Bn in 2018 and is expected to expand at a CAGR of 5.5% from 2019 to 2020. The industry is primarily driven by rise in urbanization, continuous increase in online purchase of beauty products, and growth in the adoption of social media platforms.

Levulinic acid is primarily used in personal care products and cosmetics, as it enhances active concentration; enables high water loading for moisturizing formulations; raises solubility of ethanol; and reduces greasiness. Thus, the levulinic acid market is driven by the growth of the global cosmetics industry.

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Levulinic Acid Constantly Replacing Petroleum-based Products

Levulinic acid is generally produced from cellulose, biomass, manure, and brewery waste. It is the antecedent of biofuels. Levulinic acid is widely used in the manufacturing of polyvinyl chloride, which is a better alternative for phthalate plasticizers. Levulinic acid derivatives such as gammavalerolactone (GVL), methyl tetrahydrofuran (MeTHF), and methyl butanediol (MeBDO) are suitable for use as solvents in several applications.

High Prices of Levulinic Acid to Hamper Global Market

Levulinic acid prices range between US$ 5 per kilogram and US$ 8 per kilogram. High prices of levulinic acid are associated with the high initial cost incurred for its production as well as modifications such as alterations in the production process at the application end. Various countries in Asia Pacific have imposed anti-subsidy duties on levulinic acid, leading to increase in the product price. This, in turn, restrains the levulinic acid market in the region.

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North America Dominates Levulinic Acid Market

In terms of volume, North America held a leading share of the levulinic acid market in 2018. The U.S.-based companies usually source levulinic acid at a low price from China-based manufacturers. For instance, Segetis is reported to source levulinic acid from China for its portfolio of L-ketal products.  Middle East & Africa held a minimal share of the levulinic acid market in 2018; however, the market in the region is at a nascent stage currently. It is anticipated to expand in the near future, owing to the rising demand for levulinic acid for use in plasticizers and as a substitute for petroleum-based products.

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High Application of Levulinic Acid in Agriculture

Among applications, the agriculture segment held a major share of the levulinic acid market in 2018. Delta-amino levulinic acid (DALA), a levulinic acid derivative, is widely utilized as herbicide for lawns and certain grain crops. Levulinic acid is used in anti-inflammatory medications, anti-allergy agents, mineral supplements, and transdermal patches. Delta-amino levulinic acid (DALA) is utilized in the diagnosis and treatment of cancer (photodynamic therapy).

Levulinic Acid Market: Prominent Players

Prominent players operating in the levulinic acid market include GFBiochemicals Ltd., Biofine Technology LLC, Tokyo Chemical Industry Co., Ltd., Godavari Biorefineries Ltd, and Aurochemicals. In February 2016, GFBiochemicals acquired Segetis, a player based in Golden Valley, Minnesota (the U.S.). The latter is a leading producer of levulinic acid derivatives and it owns more than 50 patents. It has over 200 pending patent applications worldwide. GFBiochemicals has acquired Segetis’ pilot production plant located in Minnesota. With this acquisition, the company is planning to establish its direct presence in the U.S. market through opening commercial offices as GFBiochemicals Americas. Biofine Technology LLC is a technology holding company controlling patents for the levulinic acid technology, along with various license agreements for related derivatives.

The Levulinic Acid market has been undergoing some remarkable growth dynamics, shaped increasingly by digital technologies and environmental sustainability concerns of product development. Over the decades, the feedstock variability and the onslaught of cheaper substitutes have squeezed in profit margins of the chemical and materials sector. On the other hand, the value chain of several businesses in the Levulinic Acid market has undergone overhaul, increasingly rendering some aspiring players with greater control over the forces and demand. The penetration of Industry 4.0 in in the market has stirred game-changing trends for range of stakeholders in the overall chemicals and materials sector. Manufacturing and production environments of businesses in the Levulinic Acid market are keen on formulating and implementing strategic frameworks that will turn them into digital enterprises.

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