#SMI indicator

[signode-blog]

How to Trade Stochastic Momentum Index: A Comprehensive Guide

Trading in the stock market can be a daunting task, especially with the multitude of technical indicators available to traders. One of the lesser-known but highly effective indicators is the Stochastic Momentum Index (SMI). This tool can be incredibly beneficial for traders looking to refine their strategies and make more informed decisions. In this comprehensive guide, we’ll explore what the…

[stirideactualitate]

SMI Ergodic Oscillator - indicator MetaTrader 5

The SMI Ergodic Oscillator indicator displays the difference between the True Strength Index and its exponentially smoothed signal line. It has three adjustable parameters: Long Length – primary smoothing period for TSI calculation Short Length – secondary smoothing period for TSI calculation. Signal Line Length – period of signal line smoothing for SMI calculation. The ergodic oscillator gives…

[crypto28ro]

SMI Ergodic Indicator & Oscillator: Ghid Complet pentru Evaluarea Impulsului și Volatilității Pieței

SMI Ergodic Indicator & SMI Ergodic Oscillator: Ghid Complet pentru Evaluarea Impulsului și Volatilității Pieței Introducere În analiza tehnică modernă, indicatorii de moment sunt folosiți pentru a evalua puterea și direcția trendurilor. Dintre aceștia, SMI Ergodic Indicator și varianta sa, SMI Ergodic Oscillator, au câștigat popularitate datorită capacității de a combina semnalele de trend cu…

[aeyumicore]

misty invasion - lost oasis

━ .ᐟ✧ PAIRING: sylus x female reader (afab)

━ ✧.˖ GENRE: smut, porn with some/little plot, porn with feelings, angst

━ .ᐟ✧ WORD COUNT: 4.5k

━ ✧.˖ WARNINGS: mdni, explicit sexual content, spoilers AND alterations to ‘lost oasis’ (sylus’s misty invasion card), slight predator and prey, dom!sylus for the most part (though he shows vulnerability), use of Y/N, sub!reader but she teases doe, unprotected sex, cumming in coochie, against the wall sex, shower sex, hand play/kink, belly bulge, finger sucking, fingering, biting, slight angst, lots of hickeys (m! And f!receiving), allusions and predictions to sylus’s lore 

━ .ᐟ✧ LINKS: video | ao3 | twitter art | xav's version | raf's version | zayne's version

━ ✧.˖ A/N: hiii second part of the misty invasion series is here <3 this time our very own birb – sylus! Next will be Xavier but I don't have a timeline for it! Could be 3 days could be a week :’) will try and keep you guys updated

Small Sylus rant, feel free to skip this and read the fic!

I have huge problems with the hypersexualization of Sylus, from the devs not from fans. I feel like the devs sometimes use him as fan service. I felt that was especially true in this card, the shower scene felt out of place and didn’t feel intimate because they’ve done far too little mc/sylus building and sylus lore. I wished the ending scene in the hammock was the cut scene, even if there was no spice. For that reason, I’ve SLIGHTLY altered the dialogue and memory, especially at the end. I added in some of my own angst, heavily influenced by predictions to Sylus’s lore I’ve seen on Twitter, especially the twitter art i linked above.

Don’t get me wrong I love Sylus. I just wish we got to see MORE of his lore and backstory, because you just KNOW it's tragic. His myth cards were nothing like the other 3 boys, and I feel like they have a lot of opportunities to help Sylus “catch up” to the other 3 LIs (could’ve done a event similar to Rafayel’s bday event, waited until they could release more main story, etc), but they haven’t utilized it well. I’m sure there’s a reason why (rushed timeline, leak threats. End of the day I understand it’s a business), but as a reader/user/fan it kinda sucks. And again, I KNOW it’s coming and it will be great. But because we haven’t seen enough yet, it makes his spicy scenes seem less intimate and more fan service-y. So I wanted to add just a sprinkle of Sylus angst and story here <3 It made me cry, I hope you guys love it as much as I loved writing it. 

THIS IS MY ONLY ACCOUNT. I WILL NEVER POST MY FICS ON OTHER TUMBLR BLOGS. I WILL ONLY POST ON THIS ACCOUNT AND ON AO3.

✦ . ˖ ✧ .ᐟ ˖ nsfw | minors dni | 18+ only | minors dni | nsfw ✦ . ˖ ✧ .ᐟ ˖ .

There are moments in life where it really hits you.

That you’re in danger. 

Where your hair stands up on its ends and your legs itch with the need to bolt away. This felt like one of those moments. The way Sylus’s voice, throaty and deep, growled with evident hunger, his eyes watching you like a predator eyeing its prey. 

Only…this time, the goosebumps that painted your skin were from excitement, and the throbbing in your thighs was from desire and not fear. This time, the tight grip on your thighs, the imposing body against yours, holding you so possessively, only leaves you wanting more. 

Sylus’s arm is pushed against the wall behind your head, caging you in with his hard soapy body. His skin shines with water and the leftover suds of his shower, before the water had run out, leaving you with a wet and mischievous Sylus.

Your fingers languidly follow soapy suds that trail down his chest, not really actually wiping anything away. Sylus remains self-assured, smirking down at your shaking body as you touch him. You can feel goosebumps form where you touch him and it’s the only indication that the silver-haired man is close to losing it. 

His voice comes out deep and breathy, “Aren’t you going to do something about my hair?”

His smile is maddening, taunting you to touch him more. So you wrap your hands behind his shoulders, yanking him down to you. 

For a brief second, Sylus is taken aback, his lips parting with a surprised breath. But as quickly as it had come, Sylus composes himself. He lets himself be pulled to you, chuckling breathily. 

“Is that it, sweetheart?” 

Before you can respond, his arm releases the wall behind you. In an instant, his large hand is gripping the underside of your thighs, hoisting you up with one arm so that both your legs dangle off his strong arm, pressed against his hip. 

You yelp at the feeling of his large hands holding you against his wet and hard body, “What are you doing?! My clothes!” You can feel your deep red satin nightdress soaking up the sudsy water on his skin.

Sylus only laughs, sarcastic and deep, “Oh no. They’re wet. Now what?” 

You try to push him away, “Well you need to let me down first.” 

But Sylus doesn’t budge, shifting you so he can lean his free arm against the wall behind you, caging you in once more. Like a lost bird. 

“Doesn’t this mean you also need to learn how to humble yourself?” His cerise eyes are frustratingly playful, his eyebrows crinkled in amusement. 

You don’t respond, instead trailing your hand down his bare chest again. Your feather-like touch ghosts his collarbone, down to his thick pecs, that tremble deliciously under your fingers. From the strain of holding you up with one arm, or your touch alone, you’re unsure. 

You speak softly, trying to tease him into losing control, “I did, and this is what I see…” 

Your touch and suggestive words have Sylus breathing heavier. His pants come out raspily, sounding vaguely close to a moan. He nearly shivers at your touch, so absolutely enamored by the way your fingers claim him, barely able to withstand his primal urges to claim you.

His reaction fuels you with confidence, and you grin cheekily, “Oh? I guess I’ll have to be more gentle.” You let your hands explore more, stroking his down his marbled chest. 

Sylus grunts, his face turning away from you, contorted in tortured pleasure. His breath comes out in rapid desperate gasps. It’s so utterly rare for you to see Sylus in such a needy state, and you can’t help but tease him further. 

Your fingers touch his neck, enjoying the way Sylus is crumbling under your fingertips when normally it’s you coming undone for him, “How cute.” 

When your fingernail grazes his nipple, Sylus tenses up, a growl ripping from his throat.

He turns to you, squirming under your fleeting touch. “Hey,” he croaks. His voice is uncharacteristically hoarse, tense with desire.

“The soundproofing for this shower isn’t great. People might get the wrong idea if they hear us. At this rate, we could end up in a lose-lose situation.” 

But you continue, pinching his ear softly, grinning, “Tell me, who pinned you down and hit you?” 

Sylus’s smile falters. He knows you’re referring to the little boy from earlier, whom you’d been teaching how to fight against Wanderers, but he can’t help but think about the first time he’d been here at the desert oasis. Only that time, he was powerless. That time, he couldn’t protect you.

He quickly masks his brief moment of melancholy, smirking at you once more, “Oh, so class isn’t over yet.” You want to prod at his sudden shift in demeanor, but you can tell now's not the time. You made a mental note to ask him about it later.

Sylus holds your wrist gently, bringing your fist to his lips. His mouth pressing fleeting kisses to your knuckles, “Then can I trouble you some more…Miss?” He doesn’t wait for you to answer, hoisting you back up onto his forearm, forcing you to wrap your arms around the back of his neck for balance. He carries you in one arm to the bathroom door, before he falters, hearing the patter of footsteps outside.

“On second thought, I’ll take my lesson in here.” He walks you back to the shower, shifting your legs so that they wrap around his hips. Your back is pressed against the wet wall, Sylus’s strong arms holding you in place. 

“What should you do…if a wanderer has you pinned down like this?” His voice is sultry and suggestive, darkened eyes daring you to teach him. 

You lift your chin proudly at his taunting challenge. Your fingers trace inexplicable shapes into his chest, your nails gently and purposefully grazing his nipples. At his sharp inhale you make your next move.

“I would…go for the neck, since that’s where they’re most vulnerable.”

Sylus’s adam’s apple bobs with the anticipation of your double-edged words, “Is that so, little bird?”

You nod with confidence, “Let me show you…” You kiss up his collarbone until you read his pulsing neck, brushing chaste kisses along his jugular. Sylus’s chest heaves, and you smile against his neck in satisfaction. 

“I can’t imagine this would be…effective against wanderers,” Sylus masks the unsteadiness of his wavering voice with a layer of arrogant amusement. 

Sylus’s chest heaves, and you smile against his neck in satisfaction. Enjoying having the upperhand, “No…just you.” You softly sink your teeth into the thick muscles of his neck.

Sylus lets out a string of harsh expletives, slamming you further into the wall, his grip on the underside of your thighs digging in harshly. You shiver at the feeling of the cold wet wall tiles pressing into your satin nightdress.

Sylus lets you have your way a little while longer, enjoying the way your rapid heartbeat pounds against his wet chest, your tongue lapping circles where your teeth had sunken in. His hands shift to grope your rear under your crimson red nightdress, squeezing the plush skin there as if you were his personal stress ball. His demanding fingers find their way to your bare pussy, spreading them apart with his index finger and thumb.

“What a bold hunter you are…taunting the enemy with no…protection.” 

His words are vaguely threatening and it makes you squirm. As his fingers toy with you, you sink your teeth deeper into him to hide your pathetically lewd whimpers. Sylus hisses at your teeth nearly breaking skin, a mix of pain and pleasure he is all-too familiar with.

“You’d better watch yourself, my little hunter,” Sylus coos in your ear, fingers finding your clit and pressing down harshly, “You never know what a beast stranded in the desert might do to you.”

His words remind you of the reason he’d invited you out to this desert oasis to begin with, the woman who’d found him, and why he was returning now. Before you can ask him, Sylus is bullying his index finger into you, sliding in so embarrassingly  effortlessly.

“What now, little dove? What would you do now?” Sylus nips at the crook of your neck, where your shoulder and throat meet. His words are hot and dangerous at the shell of your ear, his finger curling inside you to reach the spongy corners of your g-spot.

You force your words out with all the strength you have, not wanting to give Sylus the satisfaction of rendering you speechless, “I w-would never – nnghh – be in this s-situation.” 

Sylus chuckles, inserting another finger, “And yet…here you are. About to be devoured.”

The imminent threat in his words makes you clench, hard. How it was possible for the tables to turn this quickly, you’d never understand. Sylus grins when he feels your gummy walls pressing down on just two of his fingers, the quivers typically indicative of how close you are. 

He pushes you harder into the wall, lips finding your earlobes as he huffs out his words, “Look at you, my dear little hunter. So beautiful when you’re helpless.”

You whine indignantly at his condescending words, wanting to retaliate. With his lips at your ear, his neck is exposed before you and you take full advantage of his vulnerability. You sink your teeth back into his pulsing neck, knowing just how much pressure is enough to have him writhing for you. 

Sylus jolts, his fingers slipping out of you and his knee buckling slightly. His grip on your thighs tightening as he hisses out in surprise. He composes himself just as quickly, straightening up and bouncing you up to readjust his possessive grip on you. 

He pushes you back against the wall, his hard abdomen pressing into your pussy. You groan when you feel your wet lips spreading against his chiseled muscles, his body pressing so forcefully into yours, your arousal smearing against him.

His thumb and index finger take your chin into his grip, still wet with your slick, pulling your face towards his. His arrogant grin is as alluring as it is infuriating, his ruby eyes swirling with a dark amusement. 

“No more mercy, little bird.”

Sylus presses his lips to yours, his fingers tightening around your chin. His kiss is demanding, nearly suffocating, in a way that makes you reel with excitement and anticipation. It’s so torrid and feverish that you almost don’t notice his Evol unraveling your arms from around his neck, bringing them to his chest. He holds you steady with his one arm, and with the other he releases your chin, taking the two of your wrists into his single free hand. His hands are so large that even just one of his hands can envelope both your wrists. 

As his tongue probes the parting of your lips, he holds your wrists, bringing them up above your head and pinning them against the wall. His fingers play with your trembling ones, tracing the lines in your palm and grazing all the way up to your fingertips. 

You feel a brief shuffle and hear the faint thud of Sylus’s towel dropping to the floor, the scorching head of his cock prodding at your entrance. You gasp into his mouth when you feel him taking the base of his erection, stroking it against your clit. You screw your eyes shut at the deliriously delicious friction, moaning into Sylus’s mouth, his tongue claiming every corner of yours. 

Sylus pulls away, his breath coming out in short rapid gasps. He leans his forehead against yours, his fingers still enclosing yours, binding your wrists together against the cold shower wall. His proximity makes it so his warm breath fans across your swollen lips, the taste of him still on your tongue. 

He looks down at you, his crimson eyes smoldering against the heat he’s so desperately trying to keep at bay for you. If it were up to him, he’d have you splayed across his lap, screaming until the whole small town in the oasis could hear you. Unfortunately, pressed up against the wall whimpering for more would have to do.

“Are you ready, sweetheart?”

You stare at him, arms restrained above your head, chest heaving in anticipation, lips swollen and parted. Sylus smiles at you. It’s that signature Sylus smirk, heart-stoppingly tender and predatory all at once. But when his glowing cerise eyes meet yours, that’s what has your breath catching in your throat. 

The tumultuous sea of red conveyed every ounce of emotion that his words couldn’t. What it meant to have you here in the desert with him, a place that once reminded him of hell. What it meant to have you here, wrapped up in his embrace, ready to do anything to make him happy, whole. With you here, it truly did feel like an oasis. 

So you murmur boldly, cheeks warming, “M’yours, Sy. Of course I’m ready.” 

Sylus’s smile falters for a second, before he growls, slamming you back into the wall, palm cupping the back of your head with one hand, and your lower back with the other, to protect you against the impact. 

“That’s my girl.” 

With those words, his swollen cock head at your entrance finally surges forward, nudging its way into your tight embrace. You cry out as Sylus curses, the both of you never getting used to how impossibly snug the fit was, almost to the point of not fitting at all. 

Sylus takes it surprisingly slow, watching your face carefully as he sinks into you inch by inch. His hand strokes your cheek, in an overwhelming show of tender affection. Something about this desert oasis had him unusually vulnerable and it was intoxicating. 

You squirm as he bottoms out, his cockhead nestled sinfully against your cervix, practically demanding entrance into your womb. His fist is pressed into the tiled wall behind your head, his knuckles white with desperation. His entire body twitches, his breath coming out in short desperate pants. You hold his face with your hands, forcing him to level with you, stroking his sharp jaw with your fingertips.

“Sylus?”

His carmine eyes dart to yours, the vast storm of his irises looking faraway and distant. But when he looks at you, his eyes soften, the sight of you grounding him to the moment, pulling him away from the agony he once endured here. 

You kiss his furrowed eyebrow, “I’m here, Sy.” 

Sylus groans, his facial features softening at your touch, your raw words making him heave with desire. 

“You are. And you’re mine,” he growls, finally moving inside you, pulling out until just his leaking tip is nestled in your warm waiting cunt. Giving you just a second to adjust, your pussy pulsing with the need to be filled once more, he slams back into you. The impact of his thrust knocks the air from your lungs, your body sliding up the wet walls of the shower.

“Sylus!” you squeal, trying to control  your voice. Sylus grunts, reveling in the sound of your pleasure, pulling out of you and rutting back into your poor quivering cunt at a rabid vigor. 

Your bodies are pressed so tightly together, that every minute movement Sylus makes causes your clit to brush against the trail of silver hair that paints his pelvis. His hot breath is in your ear, whispering the filthiest things to you.

“You like it rough, don’t you sweetheart?” he nips your neck, savoring the taste of your clean showered skin against his insistent tongue. Your bare shoulders are already littered in his claiming marks, beautiful red bruises forming where his lips raze like wildfire. Sylus’s eyes glimmer with satisfaction at the sight of it. 

“C-can’t take it,” you whine, fingernails digging into his shoulders. His passionate thrusts are demanding, almost mean, as they try to pull moan after moan out from your lips. Your pussy quivers, already shivering from the amount of pleasure Sylus is able to force on you, so much that it spills over. 

“Yes you can, hm?”

He hisses when your nails dig further into his skin, leaving beautiful little red crescents on his muscled shoulders. Sylus always thought it was utterly insane how you knew just how much pain to mix with his pleasure to have him unhinged, just enough to want to devour you. 

You find your wrists being bound above your head again, his hand pushing them against the wall. His forehead pushes against yours as his lips desperately seek yours, capturing you in a breathtaking and fiery kiss of unspoken feelings. A torrid storm of the way he’d missed you desperately on his trip away, so much so that he had to use the little boy wanting to learn to fight wanderers as an excuse to fly you out to him. 

He pulls away, leaving you both panting for air. As he continues his feverish ruts into you, he huffs into your ear, “You can take it. You’re my good little dove, right?”

The look of complete and utter desperation in his eyes makes you want to give into every wish and whim of the silver-haired man before you. So you nod obediently, closing your eyes in satisfaction when his fingers rub soothing circling into your palms. It’s a jarring contrast, the way his hand caresses you affectionately while his cock ravages you relentlessly. It makes you delirious with ecstasy, and your body is no different.

Your cunt throbs with the need to come undone, the coil tightening so tightly that your abdomen threatens to burst. From the pleasure of his touch or from his massive cock seemingly trying to find its way into your throat, you’re unsure. 

“S-so deep,” you cry, digging your nails into his hand as it holds yours in place. Your back slides up the wall at every one of his deliberate pointed thrusts, a mere ragdoll to his ravenous hunger against the cold dripping wall.

Sylus, groans. You feel a slight shift in energy, and Sylus moving beneath you. But your position against the wall doesn’t change. Sylus’s Evol gently grips your thighs, keeping you suspended as his arm that held you up is now free to press down on your tummy. 

“I know, doll. Can you feel me all the way here?” he draws his words out seductively, pressing down on where your walls bulge against your pulsing naval. 

You squeal at the overstimulation of him physically pressing your cunt down onto his cock that still spears in and out of you wildly. Sylus removes his hand to press it against your lips, his index and middle fingers slipping into your lips that are still parted mid-scream. 

His digits press down on your tongue, faintly tasting like his expensive body wash, “Shhh, Y/N. We wouldn’t want anyone to hear, hm?”

You whine. Truth be told, the imminent danger behind his words only gets you more and more excited, closer to the edge. His forceful fingers toying with your tongue only fueled your filthy desires more, and your body reacts just as eagerly. 

Sylus bits the inside of his cheek, swearing as your tight walls convulse tightly onto him. Your pussy unknowingly constricts the thick throbbing veins that press of his erection, pressed snugly into your sweetest spots. 

“Ah, my sweet girl is so filthy. Did you like that? Like the idea of someone watching me fuck you dumb?”

His condescending words have you shaking your head, still hanging on to your slim shred of dignity. Sylus chuckles, nuzzling into your neck.

“You can’t lie to me, little bird. I can feel the truth.”

“F-feels s-ooo good,” you admit, throwing all embarrassment to the wind. Sylus swears at how adorably muffled your words are against his fingers, how your eyes are hooded with pleasure as they watch him dreamily. The adoration in your stare was so palpable, hearts nearly reflected in your eyes. His knees buckle as he admires your beautiful face, so angelically fucked out that it ruined him. 

Sylus shifts you into his single arm once more. He could easily keep you secured in the air with his Evol for hours yet, but at this moment he wants nothing more than to be as close to you as possible. He wraps one of your arms around his neck, intertwining his fingers with your other hand.

“Hold on tight, my love,” he rasps against your collar, bringing your intertwined fingers above your hand and against the wall for leverage. His thrusts take on an unprecedented intensity, the globes of your ass slapping against the wall in loud, filthy, and wet paps. His vigor makes it easy for him to hammer into your g-spot at every thrust, having you reaching the summit of your orgasm all too quickly. 

“Sy-Sylus! I-I’m so close,” you wail, fingers desperately clutching his, other hand digging into the back of his neck.

Sylus is close too, weeks of pent up emotion and need brimming to the point of boiling over. The only thing keeping him sane is the grip he has on your hand.

“Need to cum in you,” he hisses, driving into you harder as he nears his peak, “Need to breed you so full of my cum, hm?” 

You nod eagerly at his filthy words, clutching onto him for dear life, “I’m c-cum—“ 

Sylus cuts you off, smashing his lips into yours. It must’ve been a sight to behold, the way Sylus had you locked in a passionate kiss, his hand holding yours above your head, his body pushing you up against the wall, pelvis wet from your arousal as his silver hair brushes repeatedly against your clit.

It was all enough to have you finally releasing all over his defined abdomen. You squirt against his stomach, eyes squeezing shut as his tongue claims your very breath. Your climax is powerful, mind-numbing, and utterly explosive. 

Sylus huffs in pleasure as he feels your dripping thighs slapping against him, his own orgasm imminent. Your cunt continues to throb in the afterglow of your climax, wringing tightly against his violent ruts.

Finally, he climaxes inside you, moaning wildly into your mouth as he continues to devour you,  thrusting through the intense waves of pleasure. His abdomen trembles, involuntary quivers wracking his body as rope after rope of his thick seed pours into you.  

His lips pull away and he leans his forehead against yours, his entire body still quivering with waves of the intense overstimulation. His chest heaves violently with the pounding of his rapid heartbeat, his fingers still tied to yours, tracing gentle shapes in your tingling skin. 

Sylus chuckles, the sound gentle and warm and the vibrations reverberating onto your own body as he clings to you still. 

His eyes glimmer with amusement, his fingers capturing your chin again and pulling it downward to where your bodies remain connected. His voice is tender and mischievous, “It’s just like you to make such a mess when you’ve already used up all the water.”

You blush furiously when met with the image of your bodies, fit against each other together like puzzle pieces, glimmering with a wet sheen that was definitely not water. Your red satin nightdress had ridden up, the lace embroidering of the hem soaked. The argent dusting of hair on Sylus’s pelvis was matted in both your arousals. It was an absolutely sinful sight. 

“P-put me down!” you hiss, tapping his chest, “We have to find a way to clean this up.” 

Sylus raises his eyebrow at you, “Sweetheart, the only thing keeping the mess inside is my co–” 

Your hands come together to cover his mouth, “Don’t say another word.”

Sylus chuckles into your hand, his breath warm and tickling You feel a sharp, but gentle, nip.

“Hey! Don’t bite m–OW!”

—--

The night air is brisk, sounds of ancient handbells ringing out softly as the dark sky twinkles with distant stars. A nearby bonfire rages, the sound of crackling of wood mixing with the distant chime of bells. And yet, it’s Sylus’s large body holding yours that keeps you warm against the gentle night breeze of the desert. 

The hammock the two of you cuddle in swings lazily, Sylus’s fingers languidly stroking your hair as he tells you myths of the Gods and humans that once resided in this very valley. 

“What about your world? What was your life like as a kid?”

Sylus is still as his body cradles your own, his fingers crushing the blossoms that had fallen into his palm. He hesitates for a second before saying, “Nothing special. I struggled to survive.”

Your heart clenches painfully at his words. His voice is nonchalant, yet something about his words is melancholic. Eerily wistful. 

“I never imagined that one day, we’d be sitting in a place like this. Having discussions about the world,” you whisper.

You look up to catch Sylus staring at you. For a brief second, you catch the emotions in his eyes. They’re desperate, pleading with yours. For what, you’re unsure. He quickly blinks, the cerise orbs returning to their natural state.

“Do you think we’re talking about the same world?” His voice is unbearably gentle, his words confusingly cryptic, as if edged with a double meaning that you can’t quite grasp.

“I’m not sure,” you confess softly.

There’s a brief moment of silence. You continue, “Today is when people give flowers to each other in Linkon, but…”  Your voice trails off. You gently dust off the fallen petals that’d landed on his shoulder, hand reaching to brush over his heart as you pick up a branch of the delicate flowers off his abdomen.. 

“Could those flowers bloom in this kind of soil?” You ponder aloud, holding the cluster of fallen and wilting blossoms, so different from the vibrant and thriving ones you’re familiar with in Linkon.

You glance up at Sylus again. The shadows of the palm trees above you obscure one side of his face, the other half haloed by the soft glow of the moonlight. He looks threateningly ethereal. The pools of carmine in his eyes glow as they search yours. Like earlier, they glimmer with inexplicable emotions that seem to plead with yours. Begging you for…something.

But he doesn’t speak, instead taking the cluster of wilted blossoms from your fingers. He twirls them in his fingertips, inspecting them carefully. He strokes the browning petals, a strange look of nostalgia flickering across his face. 

You don’t understand, but you reach out for his hand, squeezing his fingers in yours. He squeezes you back, still looking mournfully at the flowers in his fingertips, almost as if remembering a painful memory. 

Finally, Sylus turns to you. His smile is devastatingly beautiful and tragic all at once, his finger moving to tuck the loose strands of hair behind your ear.  His piercing red eyes bore straight into your soul, the faint luminosity of his Aether core beating behind them.

“I’ve seen far more beautiful flowers bloom in this desert.”

© aeyumicore 2024.

.ᐟ✧ THIS IS MY ONLY ACCOUNT. I WILL ONLY POST ON THIS ACCOUNT AND AO3. i am not @/aeyumicores or @/aeyumiicore or any variations of my blog name.

✧.˖ i do not permit translations or reposts of my work on tumblr, ao3, or others. please do not reuse my blogpost headers, dividers, or layouts. these are original designs of my own.

[oddinary4bts]

Chasing Cars | ch 1.5 (jjk)

☆summary: when your brother goes to study on a semester abroad, your life collides with his best friend Jeon Jungkook, who's coincidentally your roommate. Will you survive the collision, or will you crumble into dust?

☆pairings: brother's best friend!Jungkook x younger sister!female reader

☆rating: 18+ (minors DNI, some chapters contain mature content)

☆genre: forbidden love?au, college!au, slice of life!au, smut, angst (as usual a lot of it), fluff

☆warnings: curses, alcohol, explicit content: jungkook has sex with a girl at the party, he overhears OC and Hoseok (no details), mentions of Jungkook knowing about the Incident

☆word count: 1.6k

☆a/n: wrote this in like twenty minutes after being unable to write for a few weeks, let's go :))))) hope you guys enjoy <3

☆join the discord server here!

☆series masterpost

☆☆☆☆☆

If I lay here If I just lay here Would you lie with me and just forget the world?

Chasing Cars, Snow Patrol

☆☆☆☆☆

Jungkook has been playing beer pong with Jimin for a while. They’re undefeated, like they most always are, and he’s buzzed with victory, but not alcohol because of course he’s not drinking tonight.

Mostly, he’s buzzed because he just beat you and the guy you were playing with, the one that looks a little too comfortable next to you. So Jungkook teases you about the game, his heart rate spiking momentarily as you blush lightly and playfully glare at him.

It’s been doing that every time he teases you since he met you last semester, and he’s grown addicted to it, which might be the reason why he teases you all the time. But then the guy interrupts, asking you if you want a drink.

“Please, I need a new drink,” you answer.

The guy smiles, and Jungkook can’t help himself. He reaches out, grabbing your arm gently to attract your attention. “I’m not drinking tonight,” he says after he let go of you. “I came with my car, thought I’d offer you a ride home.” 

You raise your eyebrows. “What?”

“As long as you don’t get sick, that is,” Jungkook teases, a smirk playing with the corners of his mouth. 

You don’t answer for a few seconds, but then you let out, “Ayt.” You move closer to him, a smile growing on your lips. “I’ll find you later?”

Jungkook is aware that you’re close now. Close enough so that he sees the way your eyes are sparkling with mischief as you look up at him, head tilted back. His tongue pushes on the inside of his cheek as he tilts his head to the side, and then he smirks as he glances at Hoseok before looking at you again.

“I’ll be waiting for you.”

He means it. He fully does, yet he forces himself to leave then, even though you looked like you might want to say something else.

Later, when you return in Jungkook’s vicinity, all he can notice is the bright red hickey that’s appeared on your neck, and he clenches his jaw, only to relax it a few seconds later. Especially as you glance at him, asking Jimin and him not to tell Taehyung about the guy you apparently were kissing a moment ago.

As the hickey on your neck seems to indicate, too.

“Lips sealed,” Jungkook says.

“Didn’t feel like playing with your friends?” Jimin asks you, not replying to your request, and Jungkook notices the two girls at the beer pong table. The pretty one meets his gaze for a few seconds - Ria, he thinks - and he smiles at her, only for her to look away immediately.

“They don’t need me,” you claim as you take a sip of what looks to be rum and coke.

“Pretty sure you’d be a liability anyway,” Jungkook jokes.

That cute blush reappears on your cheeks, and Jungkook’s heart warms for a few seconds, right as he and Jimin laugh.

“I did get the redemption shot!” you say, and Jungkook can’t help himself.

He can’t help himself but nudge you with his elbow playfully. “Doesn’t count, your little boyfriend got all the other shots for you.”

“He’s not my boyfriend!” you insist quickly.

“Of course not,” Jungkook says, cocking an eyebrow.

Jimin intervenes, “Leave her alone. She did her best.” It’s his turn to smile mischievously. “Not her fault if her best sucks.”

“Bruh,” you let out, and then you’re all laughing.

Jungkook likes this. You rarely hang out with him and Jimin, but he’s always found you fun to be around, despite the fact that you are Taehyung’s sister.

The way you occasionally make his heart race in his chest probably contributes to it.

You and Jimin start talking, and Jungkook finds himself joining in the conversation less and less. Perhaps because the pretty almond eyes of a certain Shelly have attracted his attention, even though you’re right here beside him. He excuses himself, and then he’s walking towards Shelly who smiles at him knowingly.

It’s not too long until Jungkook is buried inside of her in the upstairs bathroom, and though he usually takes his time with her, tonight he only pursues his pleasure, filling up the condom with cum before he’s made sure to please her.

Shelly doesn’t mind. She’s playful about it, teasing Jungkook, and he just kisses her deeply before pulling away. They clean up and get dressed, and when they’re ready, Jungkook grabs her hand and pulls her out of the bathroom.

Only to be met by your startled gaze the second he opens the door.

He lets go of Shelly’s hand almost instantly, holding your gaze but not saying anything. Shelly must have sensed the awkwardness slowly seeping in the atmosphere, because she leaves, not once looking back.

“Really?” you let out.

Jungkook pokes his cheek with his tongue before laughing lightly. “You told me not to fuck at home anymore.”

You purse your lips. “Didn’t expect you would respect it.”

“I’m not an asshole, peach,” Jungkook says, and it’s a little cold, a little mean. He can’t help it - it’s offensive to him that you didn’t believe him when he said he wouldn’t bring his hookups at home anymore. Like maybe his word is worth nothing, when he prides himself in trying to always respect it.

“Sorry,” you apologize.

There’s a silence, and Jungkook folds his arms on his chest as he leans against the door frame. He feels like he should say something, but he doesn’t know what, so he contents himself with looking at you as you clearly seem awkward under his gaze.

“Jimin went home,” you finally say.

A group of people appears at the top of the stairs, and Jungkook pushes up from the door frame, walking towards you. “Do you want to go home too?”

You tilt your head back so that you can meet his gaze. “You already want to go home?” You wet your lips, immediately attracting Jungkook’s gaze.

He scoffs. “I’m not drinking, I don’t see why I’d stay longer.”

“Why aren’t you drinking anyway?”

He offers you a smile. “Got morning shift at the library tomorrow.”

“Ah… Well…” You glance at the group of people as they exit the room where the coats are, and then look back to Jungkook. “My friend is supposed to sleep over, can I go find him?”

And though he was buzzing with the ecstasy from coming just a moment ago, Jungkook finds annoyance replacing it.

“Who?”

“Hoseok,” you reply.

“Ah, the guy you played beer pong with.” Jungkook smirks. “Thought he wasn’t your boyfriend.”

You furrow your brows. “He isn’t.”

“Then why are you bringing him home, peach?” Jungkook asks.

He doesn’t like your teasing answer. Doesn’t like that you tell him to imagine. Because he does. He fucking does, and he’s clenching his jaw as you all walk to his car a little later, the arctic winter night doing nothing to ease his annoyance. 

He doesn’t like the guy - Hoseok - getting a weird vibe from him that he blames on the fact that he knows you masturbated to the sounds of him and Shelly having sex a few days ago. It’s made him all too aware of you, as if he wasn’t enough already, and he doesn’t like that you’ll be with the guy in just a moment.

You reach his car, getting in, and Jungkook clenches his jaw as you choose to sit on the backseat with Hoseok. He meets your gaze in the rearview mirror, almost wanting to tell you to come sit with him in the front, but he refrains, not wanting to make a fool out of himself.

When he deems the car hot enough to drive, Jungkook starts driving, heading back home. He puts the radio on, his fingers drumming on the steering wheel to the beat of the song, up until he notices Hoseok getting closer to you on the backseat.

“Thanks for inviting me,” Hoseok tells you.

Jungkook’s fingers stop drumming on the wheel.

“Please don’t judge the state of the apartment,” you say, leaning closer to Hoseok. “This idiot doesn’t know how to -”

Jungkook almost bursts out laughing, because he’s always made sure to keep his living space clean but instead, he slams on the breaks, smiling contentedly when you and Hoseok both sit back in your respective seats.

“Sorry,” he apologizes, though he doesn’t mean it at all.

The rest of the ride is spent in silence, as Jungkook tries his best not to think about what you’re about to do. It’s sinful, to think of you that way, yet he can’t help it.

Especially not as, about twenty minutes later, he has a front row seat to the sounds of the action in your room, and his dick goes rock hard in his pants. 

He curses, ignoring his erection, instead putting on his earbuds, turning up the volume until he can’t hear you anymore. It doesn’t do anything to his imagination, which still runs wild with thoughts of you, but at least he doesn’t have to hear you anymore.

He sighs, lying in his bed, staring at the ceiling, focusing on the fixture that he’s almost never turned on since he moved in because he prefers using his LED lights. It does nothing to distract him from his arousal, so he curses again, rolling on his side.

This night promises to be a long night.

Read chapter one here!

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hope you guys liked it!! jk's not much a loverboy in this one but that's bc he's in deep denial haha :') let me know what you thought about the drabble!

All rights reserved to @/oddinary4bts, 2024. Do not copy, repost or translate.

[guccixstyless]

Idk if you are taking requests right now but if you do or if you have time could you do a freshly in love Shi Oh, like he has developed feelings for the Reader since X situation after starting to work for him or something like that.

But he is barely aware of it because he has never felt this way before, but it feels nice. At the same time he is so so obvious that has everyone trying to figure out what is going on with their boss (Reader included).

Unfamiliar Feelings

Pairing: Ryu Shio x Reader

Word Count: 1481 words.

AN: Sorry for the long wait, I hope you enjoy this one shot! xx

***********************************************************************

Ryu Shio was a man of few words, and those he spoke were usually laced with venom. His reputation preceded him; his anger was infamous, and his grumpiness a byword amongst his employees. His office was a fortress of solitude, where even the bravest of souls trembled at the mere thought of crossing its threshold.

Yet, amidst the chaos and fear, there was an unsuspecting intern who dared to tread where others feared to go. Y/N had just started her internship at Dogoo two weeks ago, with a heart full of dreams and a determination to prove herself. She navigated the corridors with caution, avoiding the gaze of her intimidating boss whenever possible.

Ryu Shio could care less about the interns. He was a busy man, the only thing he knows is to gain as much power as possible, because this was what he taught from his childhood.

That however changed once Y/N caught his attention. There was something about her that intrigued him, something that stirred emotions he had long buried beneath layers of cold indifference. She was unlike anyone he had ever encountered—bright-eyed, optimistic, and utterly oblivious to the dangers lurking in the shadows.

He observed her, smiling among her colleagues and how charmingly she greets everyone.

At first, Shio dismissed his newfound fascination as a passing fancy, a mere distraction from the monotony of his daily routine. But he couldn't shake the image of her from his mind. Her laughter echoed in his thoughts, her smile haunting his dreams.

Once his manager Kim caught Shio smiling to himself before Shio quickly composed himself.

"What's up with boss?" One of his bodyguards asked manager Kim.

"Maybe he got lead to any new deal?" he replied.

Shio left his office and was going to the research department, his eyes were searching for her but she was nowhere to be seen.

"Is it lunch break?" he asked the employees.

"Hello sir, well yes, everyone should be back in twenty minutes." one of the frightened employee replied, he was shocked to see his boss in their area, he was usually in his office and never bothered to enter this section of the office.

"Okay," he mumbled in a sour tone and left.

The employees in the department looked at each other and wondered what happened.

Once everyone was back from lunch, the sudden visit of boss became a hot topic as they all discussed about it.

"Maybe he's planning to lay off some of us," one wondered.

"Oh God, I hope not." another shivered at the thought.

As they were discussing various theories, manager Kim came and said, "Y/N boss wants to see you," in a monotone voice.

Now Y/N was scared, "why suddenly?"

"Please follow me," he ignored her question.

She entered the office and took in the surroundings, she never been to his office, she's heard no one is really allowed here except his selective few people.

She noticed Shio's chair turned back from her and around towards the window.

"Boss, she's here," manager Kim informed.

Shio spinned his chair and looked at her.

"Good afternoon, sir," Y/N greeted her boss.

"Y/N, right? Come take a seat," Shio said, briefly looking at his manager and silently indicating to leave.

His manager took the hint and bowed at him before leaving. She nervously took a seat across him.

"So, how are you adjusting to Dogoo?" he asked.

"It's going really well, thank you for this opportunity," she smiled at him.

Ah that damn smile, his heart fluttered.

"Of course, you deserve this, you're very talented, I'm happy to have you in my team." Shio said sincerely.

"Really? I mean thank you, I'll try my best," she replied.

"Listen, I want you to work from my office from now on, I have this new research thing that I want you to s=oversee," Shio smiled at her.

Wow he got really nice smile, Y/N thought to herself.

"Okay sir," she replied, she was still a bit surprised on this role though. Shouldn't senior researcher work directly under him? But she dismissed her thought and was thankful for this opportunity that will help her career.

------------------------------------------------------------------------------

It's been a month that she was working directly under Shio, she realized how much charming he was under this grumpy personality of his. He always bought her coffee and always helped her out with everything.

She would work in the morning with her team then shift to Shio's office after lunch till evening and work on his research.

Y/N found herself caught in a whirlwind of confusion. The enigmatic mafia boss who had always seemed so distant and unapproachable was suddenly paying her more attention than she knew what to do with. His glances lingered a fraction longer than necessary, his tone softer than she ever thought possible.

Her colleagues whispered behind her back, speculating about the nature of their relationship. Some envied her newfound favor, while others warned her to tread carefully, lest she find herself on the wrong side of Shio's temper.

There was another intern with Y/N named Mingyu. She quickly became best friend with him. He always cared about Y/N and they always helped each other out. One particular day they were just hanging around in the coffee machine area and were joking around. Shio was entering his office when his eyes quickly wandered over there. He scoffed and without a second thought, he marched over, his expression darkening with every step.

"What's going on here?" he demanded, his voice like thunder in the otherwise silent office.

Mingyu stiffened, caught off guard by Shio's sudden appearance. "N-nothing, sir," he stammered, taking a step back.

Shio's gaze flickered to Y/N, who was watching the exchange with a mixture of confusion and concern. "Is that so?" he said, his tone dripping with skepticism.

Y/N bit her lip, torn between loyalty to her colleague and fear of her boss's wrath. "We were just... talking," she offered weakly.

Shio's jaw clenched, his fists tightening at his sides. "I see," he said through gritted teeth. "Well, I suggest you find someone else to talk to from now on."

With that, he turned on his heel and stormed off, leaving Y/N and Mingyu in confusion.

"Was he jealous?" Mingyu wondered.

"Oh please, Mingyu, he was probably thinking why we were wasting our time," Y/N chuckled.

But for Shio, the encounter only served to confirm what he had been trying to deny all along. He was falling for Y/N, and there was no turning back.

As the days turned into weeks, Shio found himself drawn to her like a moth to a flame. He sought out her company whenever he could, relishing in the warmth of her smile and the lightness of her laughter. And with each passing day, the walls around his heart crumbled a little more, until he found himself standing on the precipice of a feeling he had long thought himself incapable of—love.

It was a feeling both terrifying and exhilarating, and he struggled to come to terms with it. He was a man of power and influence, used to getting what he wanted with a snap of his fingers. But love was a force beyond his control, a wild and untamed beast that threatened to consume him whole.

Finally, unable to bear the weight of his emotions any longer, Shio sought out Y/N in the quiet solitude of his office. She looked up from her work, surprise written across her features as he entered, his expression uncharacteristically vulnerable.

"Y/N," he began, his voice barely above a whisper. "I... I need to tell you something."

Y/N regarded him with a mixture of curiosity and concern. "What is it, Mr. Ryu?"

Shio took a deep breath, steeling himself for what was to come. "I... I think I'm in love with you," he confessed, his heart pounding in his chest.

For a moment, Y/N was speechless, her eyes widening in disbelief. But then, a smile spread across her face, radiant and full of hope.

"Really?" she whispered, her voice barely audible above the pounding of Shio's heart.

Shio nodded, his gaze never leaving hers. "Yes, really."

And with those simple words, the floodgates burst open, washing away the doubts and fears that had plagued him for so long. In that moment, Ryu Shio, the notorious mafia boss, was just a man in love, standing before the woman who had stolen his heart.

As for Y/N, she reached out and took his hand, her fingers intertwining with his in a silent promise of forever. And as they stood there, bathed in the soft glow of the city lights, they knew that their love was a force to be reckoned with, strong enough to conquer even the darkest of shadows.

[covid-safer-hotties]

Reference archived on our website

I thought maybe some of y'all would like a qualitative study over a quantitative one. A study of covid disparities in England among PoC and disabled people.

Abstract Background COVID-19 Ethnic Inequalities in Mental health and Multimorbidities (COVEIMM) is a mixed methods study to explore whether COVID-19 exacerbated ethnic health inequalities in adults with serious mental and physical health conditions. We analysed data from electronic health records for England and conducted interviews in Birmingham and Solihull, Manchester, and South London. Sites were selected because they were pilot sites for the Patient and Carer Race Equality Framework being introduced by NHS England to tackle race inequalities in mental health. Prior to the pandemic people in England with severe mental illnesses (SMIs) faced an 11–17-year reduction in life expectancy, mostly due to preventable, long-term, physical health conditions. During the pandemic there was a marked increase in deaths of those living with an SMI.

Aims This qualitative interview study aimed to understand the reasons underlying ethnic inequalities in mortality and service use during the COVID-19 pandemic for adult service users and carers of Black African, Black Caribbean, Indian, Pakistani, and Bangladeshi backgrounds living with serious multiple long-term mental and physical health conditions.

Methods We took a participatory action research approach and qualitative interviews undertaken by experts-by-experience and university researchers Participants were purposively sampled by ethnicity, diagnoses, and comorbidities across three geographically distinct sites in England. Transcriptions were coded inductively and deductively and analysed thematically.

Results Findings indicated multiple points along primary and secondary health pathways for mental and physical health that have the potential to exacerbate the unjust gap in mortality that exists for Black and Asian people with SMIs. Issues such as timely access to care (face-to-face and remote), being treated in a culturally appropriate manner with empathy, dignity and respect, and being able to use services without experiencing undue force, racism or other forms of intersectional discrimination were important themes arising from interviews.

Conclusion These poor experiences create systemic and enduring healthcare harms for racialised groups with SMIs that need to be addressed. Our findings suggest a need to address these, not only in mental health providers, but across the whole health and care system and a need to ensure more equitable healthcare partnerships with service users, carers, and communities from racialised backgrounds who are often excluded.

[pj-68]

La marcatura "SMI 916" indica che il bossolo è stato fabbricato dalla Società Metallurgica Italiana nell'anno 1916. Questa marcatura è tipica delle munizioni utilizzate durante la Prima Guerra Mondiale, in particolare quelle per il fucile Carcano Mod. 91 e il moschetto, che erano le armi standard dell'esercito italiano in quel periodo. 

The marking “SMI 916” indicates that the case was manufactured by the Italian Metallurgical Society in 1916. This marking is typical of the ammunition used during the First World War, in particular those for the Carcano Mod. 91 rifle and the musket, which were the standard weapons of the Italian army at that time.

[666writingcafe]

Barbatos' Birthday Surprise (Part Eight)

Author's Note: This is the NSFW/smut part of this little story, so if that's not your thing (or if you're underage), then keep strolling, because this is basically going to detail what Barbatos and MC do after the concert back at their hotel room, if you catch my drift. For everyone else, the content is underneath the cut.

Content Warning/Summary: everything between MC and Barbatos is consensual, rough kissing, biting, blood sucking both in reference to this and this and it actually happening, oral (giving and receiving; MC is gender neutral), mention of implied voyeurism, implied size insecurity (as a result of virgin!Barbatos) reference to this, slight degradation (but it's mixed in with praise, so do with that what you will), penetration (no condom), Barbatos morphs into his in-game demon form (so what the fandom has called level two), wrist-binding via forked tail

"MC." The moment we walk up to the door of our hotel room, any playfulness that Barbatos displayed throughout the concert disappears, replaced by a nearly overwhelming seriousness.

"What's wrong?" I ask. He runs a hand through his hair and sighs.

"I need to know if you're okay with continuing what we started earlier." Right. The concert. The music combined with the cocktail we both had may have caused us to get a little...bold as the evening progressed. I can still feel his hands on my stomach and his lips on my neck, and I wonder if he still feels me rubbing up against him.

"I wouldn't have done it if I--"

"Yes or no, MC." His eyes are brighter and more intense than usual. "I don't need a long-winded explanation." He sighs again, wincing at his harsh tone. "Look, I don't know if I'd be able to control myself once we're inside, and the last thing I want to do is to hurt you by doing something without your consent. So, let me ask you again: do you want to pick up where we left off?" His heated gaze causes me to swallow nervously, and my "yes" comes out as a mere whisper.

He allows me to enter the room first. Once he closes the door, he quickly pushes me up against it and kisses me. Hard. His tongue quickly finds his way inside my mouth, and he lifts me off my feet, forcing me to wrap my legs around his waist in order to prevent myself from falling right on my ass.

I'm sure I'll feel the bump on the back of my head in the morning, but right now, that is the last thing on my mind. In fact, there's only one word repeating itself over and over: more. Whether it's purely physical or a result of Barbatos' most prevalent sin affecting me is inconsequential at this point; I just want him to keep going.

He eventually breaks the kiss, but doesn't back away. Instead, he rests his forehead on mine and gazes into my eyes, making me feel fluttery inside.

"I want to try something," he murmurs. "Lucifer mentioned doing it once, and I wonder if it's as good as he says it is." It takes me a moment to figure out what he's referring to, but then I remember: the night we made our pact. Specifically, what happened right before.

"Okay." I tilt my head so that my neck's exposed to him.

"I'll try my best to be gentle." He leans down and licks a spot on my neck, sending shivers down my spine. He then wraps his mouth around it, and a sharp pain radiates from that spot, indicating that he's not only bitten down on it, but that he's pierced the skin. Gasping, I grip Barbatos' shoulders as the pain morphs into something else. Something that causes me to moan. Thankfully, I'm not the only one that makes a noise, for Barbatos groans as well.

"He's right," he whispers, detaching himself from my neck. "You are heavenly." He licks the spot again before glancing up at me. "Would you want some of mine?" The question initially takes me aback, but I quickly remind myself that it is common for demons to drink each others' blood when they get intimate.

"As much as I appreciate the offer, I'm not sure if doing so would be the safest thing for me to do." A soft smile crosses his lips.

"Of course. How silly of me. Can't have you turning into something else, after all." He pauses. "Although, there's nothing that says you can't still leave your mark on me."

"You want me to give you a hickey?"

"I hardly think just one would suffice." Is he being serious right now?

"You do realize that we're due back tomorrow, right?" Barbatos shrugs.

"I like to think that I'm good at hiding things, especially from the Young Master." He gently helps me get off him before stepping back and extending a hand out to me. "Plus, it hardly seems right for you to be the only one with visible evidence of our little tryst, now does it?"

"You're ridiculous," I mutter as I grab his hand. He guides us to the couch and sits down, instructing me to straddle his lap and face him.

"Are you sure about this?" I ask. Barbatos gently smiles, patting my thigh.

"Yes, MC. I'm sure." Gingerly, I lean down and kiss his neck.

"It's okay," he coaxes, sensing my hesitance. "You won't hurt me." He begins rubbing his hand up and down my thigh. His touch and his scent are enough to make me feel less nervous, leading me to start sucking and nipping at his neck.

"You can go harder than that," he murmurs. Somehow, despite the two of us not having a pact with each other, my mind and body treat his words like a command. Soon, the sound of his pleasure-filled groans fill my ears, spurring me to go even harder.

"Good human." Should I care that he said that, given that demons normally use the word 'human' as an insult? Probably. Do I? Absolutely not. Especially not by the time our shirts are off and our hands are roaming each other's bodies as we resume kissing.

I can't help it. It feels nice.

After a while, Barbatos breaks the kiss and places a hand on the side of my face.

"I think it's time to move things to the bedroom," he states quietly. "The couch isn't suitable for what I want to do to you."

"Okay." He stands up, forcing me to wrap my legs around him again as he easily carries me to the bedroom. I know demons are a lot stronger than humans, but I didn't think he'd be able to pick me up with as much ease as he seems to have. I'm not exactly the lightest person in the world.

Once he's put me down on the bed, he straddles me and gently pushes me backwards, making my head hit the soft pillows. He plants a quick kiss on my lips before slowly moving down my body. By the time his hands reach the waistband of my jeans, I've been reduced to pants and gasps.

"Am I good to continue?" he asks.

"Yes." With that, he unbuttons my jeans and slides them down my legs, my underwear soon following. His eyes train on my face as he begins rubbing his hand on me, intent on my reaction. My quiet pants morph into louder whines as he continues. That seems to satisfy him, for his hand quickly gets replaced by his mouth. He begins humming as he tastes me, almost as if I'm one of his favorite desserts or teas.

At some point, my good judgement ends up clouded by how good he feels, and I end up uttering an 'oh god'. Like an idiot. I mean, what kind of human says 'oh god' to a demon? Unsurprisingly, Barbatos stops what he's doing, but rather than scold me, he simply replies,

"He wouldn't make you feel this good." As if to prove his point, he resumes with a renewed fervor. Soon, I'm moaning in time with my mind chanting his name. The added noises he's making push me over the edge, and my hands grip the sheets as pleasure overrides my senses.

Once I've managed to calm down, I sit up and stretch my arms and legs before focusing on Barbatos, who's hardly moved an inch.

"Come here," I tell him. Wordlessly, he obeys, and soon our positions are switched.

"I haven't done this before," he murmurs.

"What, received a blowjob?" He blushes.

"To be honest, any of this."

"Then how--"

"By watching."

"Does Diavolo--"

"Yes. He has me hide in the shadows." He pauses. "There are plenty of people that would use sex as an opportunity to hurt him, and part of my job is to protect him."

"So, even--"

"Not you two. We know neither of you wish him any harm." I didn't realize his duties extended to those type of activities, but in a way, it makes sense. I know Asmo uses his lust as a weapon at times, so what's to stop other demons from doing the same?

"Anyway, I just don't want you to be...well..." Barbatos trails off.

"Disappointed?" I offer. He meekly nods. Did not realize he was insecure about that of all things. I pat his thigh, trying to reassure him.

"I'm a human, remember? Anything you have is going to be a lot larger than what's normally available for me. Besides, I wouldn't change my opinion of you just because of what you may or may not have downstairs. I'll still like you." With that, I remove his pants to reveal...

Wow.

I can't even form words.

And I don't mean that in a bad way.

Quite the opposite, actually.

"MC?"

Right. Barbatos. Have to make him feel good.

He hisses as I wrap my mouth around him.

"Shit."

This isn't the first time my brain has short-circuited like this. That was with Asmo. He was walking around with no clothes on, not realizing that I was home.

There was a little voice in my head that kept repeating "suck it". I couldn't focus on anything else. The only way I could return back to normal was if I did what the voice told me to do.

Just like now.

Except it's more intense. Probably because this time, it's intentional.

Barbatos groans. I hum in response.

"Fuck, MC."

I want more. I don't care if I choke.

More groaning.

More cursing.

Soon, I feel him shoot down my throat. Of course, I swallow.

He's still hard.

I barely have time to register this before Barbatos grabs me and flips me over so that he's on top again. Moments later, he's entering me.

"You'll have to forgive me. I simply could not resist."

After a few careful thrusts, he begins picking up the pace.

"You feel so good, MC. I understand why everyone's so obsessed with you; you're like the perfect little toy."

Again, I probably should be offended, but I can't find it in me to protest. Not when my mind is turning to mush with each thrust.

A sharp pain indicates that he's bitten down on my shoulder, but somehow that only adds to the overall experience. My hands find their way up in his hair.

His horns have sprouted.

Where's the tail?

My question gets answered quickly, as I feel something wet wrap around my wrists and yank them up.

Speaking of wet...

There's lots of squelching. It mixes well with the other noises, both verbal and otherwise. Asmo would be impressed.

Barbatos' movements become more erratic, which means he must be getting close. Sure enough, he bottoms out moments later as my insides become soaked.

"Are you okay?" Barbatos asks, unwrapping himself from me. "I didn't go too hard, did I?"

"I'm still in one piece, so I'll say no." My joke appears to sail over his head, for he remains serious. "Look, considering that you morphed, you did just fine."

"I did bite you, though." He's spiraling. I can tell.

"Barbatos, quit beating yourself up. I enjoyed myself. Immensely." I reach over and hug him. "I'm glad that I could be part of your birthday present."

"Thanks," he breathes as he hugs me back. We remain still for a few moments as we bask in each others' warmth. Once we let go, he grabs my hands and looks into my eyes.

"MC, would you want to...maybe...take a bath with me?" He quickly adds,

"I promise I won't attempt to go another round. That is, not unless you want me to. It's just that..." He pauses as he takes a deep breath. "If you're okay with it, I'd like to help you clean up." He seems sincere, unlike a certain other demon I know.

"Only if you let me do the same to you."

"Of course."

[crypto28ro]

Stochastic, SMI și Stochastic RSI: Ghid Complet pentru Identificarea Condițiilor Extreme și a Schimbărilor de Trend

Stochastic, SMI și Stochastic RSI: Ghid Complet pentru Identificarea Condițiilor Extreme și a Schimbărilor de Trend Introducere În analiza tehnică, indicatorii de momentum joacă un rol esențial în evaluarea condițiilor pieței și în identificarea momentelor de inversare a trendului. Trei dintre cei mai populari indicatori care se bazează pe conceptul de stochastic sunt Stochastic Oscillator,…

[anviduby]

Asthma Treatment Market Research: Environmental Considerations and Sustainable Inhaler Innovations

The global asthma treatment market is undergoing significant transformation, driven by evolving regulatory frameworks and rapid technological advancements. These factors are reshaping how asthma is managed, from drug development to patient care, leading to improved outcomes and a more patient-centric approach.

1. Environmental Impact of Traditional Inhalers

Metered-dose inhalers (MDIs) have been the cornerstone of asthma treatment for decades. However, they utilize hydrofluoroalkane (HFA) propellants, potent greenhouse gases with a global warming potential significantly higher than carbon dioxide. Studies indicate that each MDI can emit between 9 to 48 kg of CO₂ equivalent over its lifetime, comparable to the carbon footprint of a pound of beef .

In the United States, MDIs accounted for 70% of inhaler prescriptions in 2020, contributing to a substantial portion of healthcare-related greenhouse gas emissions . The environmental concerns associated with MDIs have prompted healthcare systems to seek more sustainable alternatives.

2. Sustainable Alternatives: Dry Powder Inhalers (DPIs)

Dry powder inhalers (DPIs) have emerged as a more environmentally friendly option. Unlike MDIs, DPIs do not require propellants, resulting in significantly lower greenhouse gas emissions. The carbon footprint of DPIs is estimated to be 18 times smaller than that of MDIs, primarily due to the absence of propellant gases .

Countries like Sweden have successfully transitioned to DPIs, with only 10% of inhalers being MDIs by 2019. This shift has not only reduced carbon emissions but also improved asthma control among patients .

3. Pharmaceutical Industry's Response

Recognizing the environmental impact, pharmaceutical companies are investing in developing low-emission inhalers. AstraZeneca has completed clinical trials for Breztri, an inhaler using a propellant with near-zero global warming potential, with regulatory filings submitted in Europe, the UK, and China .

Similarly, GSK is developing a low-carbon version of Ventolin, aiming to reduce its carbon footprint by over 40%. These initiatives align with global efforts to phase out high-GWP propellants and promote sustainable healthcare practices .

4. Challenges in Transitioning to Sustainable Inhalers

Despite the environmental benefits, the transition to sustainable inhalers faces several challenges:

Cost Considerations: DPIs and soft mist inhalers (SMIs) can be more expensive than MDIs, potentially limiting accessibility for patients, especially in low-resource settings .

Patient Adaptation: Some patients may find it challenging to switch to DPIs due to differences in inhalation techniques or device handling.

Healthcare System Constraints: In regions without strong environmental regulations, there may be less incentive for healthcare providers to adopt sustainable inhalers.

Addressing these challenges requires coordinated efforts among healthcare providers, policymakers, and pharmaceutical companies to ensure that sustainable inhalers are accessible and effective for all patients.

5. The Role of Recycling and Disposal Practices

Proper disposal and recycling of inhalers are crucial in mitigating their environmental impact. Inhalers should not be disposed of in regular trash due to the residual propellant gases they contain. Instead, they should be returned to designated collection points for appropriate recycling.

Recycling programs can recover valuable materials from inhalers, such as metals and plastics, reducing the need for raw material extraction and minimizing waste .

6. Future Outlook

The future of asthma treatment lies in balancing effective patient care with environmental sustainability. Innovations in inhaler technology, such as the development of biodegradable materials and energy-efficient manufacturing processes, hold promise for further reducing the ecological footprint of asthma treatments.

Additionally, increasing public awareness and education about the environmental impact of inhalers can empower patients to make informed choices and participate in recycling programs.

Conclusion

The environmental considerations associated with asthma treatments are becoming increasingly important in the global effort to combat climate change. While MDIs have been instrumental in managing asthma, their environmental impact necessitates a shift towards more sustainable alternatives like DPIs and SMIs. The pharmaceutical industry's commitment to developing low-emission inhalers, coupled with effective recycling practices and patient education, can significantly reduce the ecological footprint of asthma care. By embracing these innovations, the healthcare sector can contribute to a healthier planet while continuing to provide effective treatment for asthma patients.

[jamadhann]

Best Stock Market Classes in Jaipur

The Best Stock Market Institutes and Training Courses in Jaipur The stock market is a dynamic and rewarding field that attracts many aspiring investors and traders. However, to navigate the complexities of the market and make informed decisions, it is essential to acquire proper training. Jaipur, a city known for its rich culture and history, is also home to some of the best stock market institutes that provide top-notch education for aspiring traders and investors. Whether you are looking to delve into the basics of stock trading or learn advanced concepts like technical analysis and options trading, Jaipur offers a range of institutes that cater to your needs. In this article, we will explore the best stock market institutes, courses, and training opportunities in Jaipur. Why Choose a Best Stock Market Classes in Jaipur? Jaipur has become a hub for education in recent years, with numerous institutes offering courses across different disciplines. Stock market education is one of the growing fields in the city, with students and professionals eager to invest in financial markets. A structured stock market course in Jaipur can offer several benefits:

Expert Guidance: Stock market institutes in Jaipur are staffed by experienced professionals who bring years of practical knowledge and insights.

Comprehensive Curriculum: The best courses cover a wide range of topics, from stock basics to complex strategies like options trading and technical analysis.

Personalized Attention: Many institutes provide one-on-one training or small batches, ensuring each student receives individual attention.

Hands-on Experience: Practical training through mock trading and real-time market analysis helps students gain confidence and make informed decisions.

Networking Opportunities: Being part of a stock market class allows you to connect with like-minded individuals and industry experts. Now, let’s dive into some of the best stock market institutes and courses that Jaipur has to offer. Best Stock Market Institutes in Jaipur When looking for the best stock market institutes in Jaipur, it is important to consider factors such as the quality of instructors, the curriculum, hands-on experience, and post-course support. Here are some top institutes that stand out:

The Financial Academy (TFA) The Financial Academy (TFA) is one of the leading stock market training institutes in Jaipur. Known for its comprehensive curriculum, TFA offers various programs that cover everything from the basics of stock trading to advanced concepts like technical analysis and options trading. They offer: • Stock Market Basics: Ideal for beginners, this course covers the fundamental aspects of the stock market, including trading strategies and risk management. • Technical Analysis Course: TFA’s Technical Analysis Course in Jaipur teaches students how to analyze stock price movements using charts and indicators. It is designed for those who want to become professional traders. • Options Trading: For those interested in option trading, TFA offers the best option trading course in Jaipur, which includes practical strategies for hedging, speculation, and arbitrage. TFA is well-known for its expert instructors and a vast network of successful alumni. They also provide post-course support to help students implement their learnings.

Stock Market Institute of India (SMI) Stock Market Institute of India is another reputed institution in Jaipur that provides high-quality training in stock trading and investment strategies. With a focus on both fundamental and technical analysis, SMI offers several courses that cater to traders of all skill levels: • Best Stock Market Classes in Jaipur: These classes cover topics such as stock analysis, portfolio management, and investment strategies. • Technical Analysis Course in Jaipur: SMI offers in-depth training on technical analysis, teaching students to read and interpret stock charts, identify trends, and develop profitable trading strategies. • Advanced Option Trading: SMI offers an advanced options trading course designed to equip students with the knowledge of complex strategies like spreads, straddles, and strangles. SMI’s hands-on approach, real-time market scenarios, and comprehensive learning materials make it one of the best options for stock market education in Jaipur.

Share Market Institute of Jaipur (SMI Jaipur) Share Market Institute of Jaipur is another top choice for individuals looking to build their careers in the stock market. They offer practical training and expert guidance in various aspects of stock trading: • Best Share Market Training Institute in Jaipur: SMI Jaipur’s training programs include in-depth sessions on stock analysis, investment strategies, and financial markets. • Courses in Technical Analysis: SMI Jaipur’s technical analysis course is designed to help students understand price movements, patterns, and indicators that predict market trends. • Option Trading: SMI Jaipur also offers specialized training in options trading, including strategies for beginners as well as advanced traders. SMI Jaipur provides comprehensive learning resources, live trading sessions, and expert guidance to help students succeed in the stock market.

NSE Academy Jaipur The National Stock Exchange (NSE) Academy offers professional stock market courses in Jaipur. Being an institution backed by India’s largest stock exchange, the NSE Academy offers credibility and expertise in stock market education. Some of the notable offerings include: • Certification Programs: NSE Academy provides certification programs in technical analysis, financial markets, and stock trading. • Advanced Options Trading: For those interested in options trading, the academy offers an advanced course covering topics such as options pricing, volatility, and strategy execution. • Technical Analysis Training: Their technical analysis course provides hands-on experience with real-time data, which helps students learn how to interpret stock charts and make informed trading decisions. NSE Academy’s association with the stock exchange adds immense value, making it a trusted institute for stock market education.

EduPristine Jaipur EduPristine is a well-known brand in the education sector, offering a wide range of financial and stock market-related courses. They provide comprehensive training for individuals looking to make their mark in the stock market: • Best Stock Market Course in Jaipur: EduPristine offers a comprehensive stock market course that covers both technical and fundamental analysis. • Option Trading Course: The institute offers the best option trading course in Jaipur, focusing on risk management strategies, advanced trading methods, and options pricing. • Technical Analysis Course: EduPristine’s technical analysis course helps students understand complex chart patterns, indicators, and market trends, which are essential for successful trading. EduPristine is known for its structured curriculum, experienced trainers, and student-friendly environment. Choosing the Best Stock Market Course in Jaipur When selecting the best stock market course in Jaipur, there are several factors to consider: • Instructor Experience: Look for courses taught by experienced professionals who have a proven track record in stock trading. • Curriculum: Ensure that the course covers a wide range of topics, including stock market basics, technical analysis, and options trading. • Hands-on Training: Practical training through live market scenarios and mock trading is essential for building confidence and gaining real-world experience. • Student Support: The best stock market institutes provide post-course support, including mentorship, networking opportunities, and resources to help students succeed. Conclusion Jaipur is home to some of the best stock market institutes and training programs in India. Whether you are a beginner looking to learn the basics of stock trading or an advanced trader seeking to specialize in options trading or technical analysis, the city offers a wealth of opportunities. Institutes like The Financial Academy, Stock Market Institute of India, and Share Market Institute of Jaipur provide comprehensive courses, expert guidance, and hands-on experience to help you succeed in the stock market. By choosing the right course and institute, you can equip yourself with the necessary skills and knowledge to navigate the complexities of the stock market and build a successful trading career.

[curiousmarketersclub]

From the abstract of the paper Do Influencers Influence? A Meta-Analytic Comparison of Celebrities and Social Media Influencers Effects 📝

Findings reveal that social media influencers (SMIs) are more effective than brand-only advertising and that there is no significant difference between SMIs and celebrity endorsers. Taking these factors into consideration, the effects are moderated by perceived credibility and influencer types, indicating that mega-influencers are relatively more persuasive, while nano-influencers are less persuasive compared to celebrities, respectively. Findings imply that there is a “sweet spot” wherein SMIs are most effective and distinct from celebrity endorsers, providing support for more nuanced conceptualizations of SMIs and calling for future research to explore additional enhancing and attenuating factors.

[silentheartattack]

Silent Heart Attack

Rishab Multispeciality Hospital is equipped to treat symptoms and treatment of heart conditions, ensuring that patients receive the most care possible throughout their treatment journey. Dr. Ashish Gupta is a Senior Interventional Cardiologist at Rishab Multispeciality Hospital in Jagatpura. As the best heart care hospital in Jaipur, we evaluate a patient's medical history, perform physical examinations, and perform diagnostic tests using an Electrocardiogram (ECG or EKG) to diagnose heart conditions. Our highly skilled interventional cardiologist and team deliver superior care and optimal outcomes for patients with complex cardiac conditions.

A silent heart attack, also known as a silent myocardial infarction (SMI), is a heart attack that occurs without the typical or noticeable symptoms associated with a heart attack. People who experience a silent heart attack may not realize they are having one, which can lead to delayed treatment and increased risk of future cardiovascular problems.

Causes:

The causes of a silent heart attack are largely the same as a typical heart attack:

Coronary Artery Disease (CAD): This is the primary cause of heart attacks. Plaque buildup in the arteries restricts blood flow to the heart, leading to a heart attack.

Atherosclerosis: The buildup of cholesterol and fatty deposits in the arteries can block blood flow to the heart muscle.

High Blood Pressure: Chronic hypertension can damage arteries, making them more susceptible to plaque buildup.

Diabetes: Diabetics have a higher risk due to damage to blood vessels and nerves, which can affect pain sensitivity, leading to a heart attack going unnoticed.

Smoking: Smoking accelerates the development of atherosclerosis and other heart-related conditions.

Obesity and Sedentary Lifestyle: These increase the risk of heart disease and subsequent silent heart attacks.

Stress: Chronic stress can contribute to increased blood pressure and heart disease.

Family History: Genetics can play a role in predisposing individuals to heart disease and heart attacks.

Symptoms:

Unlike a typical heart attack with intense chest pain, a silent heart attack often presents with vague or mild symptoms, or sometimes no symptoms at all. Symptoms may include:

Mild Discomfort or Pain:

In the chest, back, jaw, or arms.

It may be mistaken for indigestion, muscle strain, or fatigue.

Shortness of Breath:

Even without physical exertion.

Fatigue or Weakness:

Feeling unusually tired, especially with no clear cause.

Nausea or Lightheadedness:

Often dismissed as a minor issue, but it could signal a heart problem.

Cold Sweats:

Unexplained sweating may occur.

Heartburn or a Sense of Pressure:

People might think it's a digestive issue, but it could be heart-related.

Because these symptoms are subtle, many people either ignore them or attribute them to other conditions, increasing the risk of more serious complications if untreated.

Diagnosis:

Silent heart attacks are often discovered incidentally during tests for other conditions. Diagnostic methods include:

Electrocardiogram (ECG/EKG): This can reveal signs of past heart damage.

Blood Tests: Elevated levels of certain enzymes (such as troponins) can indicate heart muscle damage.

Echocardiogram: This ultrasound test shows the heart's movement and can detect areas with poor blood flow or damage.

Stress Test: Evaluates how the heart functions during physical exertion.

Coronary Angiography: An imaging test that helps visualize blockages in the coronary arteries.

Treatment:

Once diagnosed, the treatment of a silent heart attack is similar to that of a typical heart attack. It aims to prevent further heart damage, improve heart function, and reduce the risk of future heart attacks.

Medications:

Aspirin: Reduces blood clotting, preventing further artery blockages.

Statins: Lowers cholesterol and helps reduce plaque buildup in the arteries.

Beta-blockers: Helps reduce heart rate and blood pressure.

ACE Inhibitors: Helps relax blood vessels and improve blood flow.

Blood Thinners: Prevent further clotting.

Lifestyle Changes:

Diet: A heart-healthy diet low in saturated fats, cholesterol, and sodium.

Exercise: Regular physical activity strengthens the heart and improves circulation.

Smoking Cessation: Quitting smoking greatly reduces the risk of heart disease.

Weight Management: Maintaining a healthy weight lowers the burden on the heart.

Surgical Interventions:

Angioplasty and Stenting: To open blocked arteries and place a stent to keep them open.

Coronary Artery Bypass Grafting (CABG): A surgery that improves blood flow by bypassing blocked arteries.

Prevention:

Preventive strategies focus on managing risk factors to reduce the likelihood of both silent and traditional heart attacks:

Monitor Blood Pressure and Cholesterol Levels: Regular check-ups and maintaining levels within healthy ranges are key.

Control Blood Sugar Levels: Particularly for those with diabetes or prediabetes.

Healthy Lifestyle Choices: A balanced diet, regular exercise, stress management, and avoiding tobacco are critical.

Medications: For people at high risk of heart disease, medications like statins or blood pressure-lowering drugs may be prescribed as a preventive measure.

Prognosis:

People who have had a silent heart attack are at higher risk for subsequent heart problems. With timely diagnosis and management, however, many individuals can lead healthy, active lives. Regular medical check-ups are essential for monitoring heart health, especially for those with risk factors for heart disease.

Conclusion:

While a silent heart attack may not have dramatic symptoms, it is just as dangerous as a typical heart attack. Awareness of subtle signs, understanding your risk factors, and maintaining a heart-healthy lifestyle can make a critical difference in preventing and managing this condition.

[covid-safer-hotties]

SARS-CoV-2 can infect and replicate in human motor neurons differentiated from induced pluripotent stem cells - Published Jan 4, 2024

Numerous patients experience neurological and neuromuscular symptoms during and after infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Apart from the central nervous system (CNS), the peripheral nervous system (PNS) is also affected. In this study, the Italian authors investigated whether SARS-CoV-2 can infect and replicate in human motor neurons (MNs) differentiated in vitro from induced pluripotent stem cells (iPSC-MNs). They also examined whether iPSC-MNs express the main receptors for SARS-CoV-2 entry and whether SARS-CoV-2 infection of iPSC-MNs changes the expression of 46 genes involved in cell survival, metabolism, inflammatory response, apoptotic and antiviral pathways.

SARS-CoV-2 is an enveloped, positive-sense, single-stranded RNA virus. Its genome encodes four structural proteins, namely the spike (S), envelope (E), nucleocapsid (N), and membrane (M) proteins. It seems that SARS-CoV-2 uses various neuroinvasive strategies and entry pathways to invade the nervous system, such as infection of the nasal olfactory epithelium and axonal transport along the olfactory nerve, retrograde axonal transport, invasion by compromising the blood-brain barrier, retrograde virus spread from the lungs to the CNS via the vagus nerve, and the use of infected hematopoietic cells as “Trojan horses” (hematogenous route).

It remains unknown whether observed neuromuscular manifestations of SARS-CoV-2 infection are caused by a direct viral invasion of motor neurons, and/or are a collateral injury resulting from an uncontrolled innate immune response. Damage to motor neurons leads to the deterioration of muscle function, manifested as muscle weakness, atrophy, or paralysis.

Two host-cell factors are important for SARS-CoV-2 viral entry into many cell types: angiotensin-converting enzyme 2 (ACE2), which is bound by the S protein, and transmembrane serine protease 2 (TMPRSS2), which cleaves the S protein, allowing this binding to take place. In addition to ACE2 and TMPRSS2, the S protein has been reported to engage other cell-surface factors proposed to serve as attachment factors promoting SARS-CoV-2 entry.

The expression level of ACE2 is low in the human brain. In contrast, neuropilin 1 (NRP1), CD147, TMPRSS2, and furin are higher and broader expressed than ACE2, indicating that they may be putative mediators of SARS-CoV-2 entry into human nervous system cells. According to the authors of this study, the infection of human motor neurons with SARS-CoV-2 mainly relies on CD147 and/or NRP1 binding. Previous studies have shown that NRP1, known to bind furin-cleaved substrates, potentiates SARS-CoV-2 infectivity and that the furin-cleaved S1 subunit of the S protein binds directly to cell surface NRP1. www.science.org/doi/10.1126/science.abd2985

About the study The authors used an in vitro model of human motoneurons (MNs) differentiated from induced pluripotent stem cells (iPSC-MNs) to investigate the infectability of these cells by SARS-CoV-2 and their expression of the main SARS-CoV-2 receptors. They also examined whether SARS-CoV-2 changes the expression of 46 genes involved in cell survival, metabolism, inflammatory response, and apoptotic and antiviral pathways.

The induced pluripotent stem cells (iPSCs) from three healthy donors (1 male and 2 females, aged between 37 and 49 years) differentiated into motor neurons that expressed both, neuronal (bIII-tubulin and SMI-312) and motoneuronal (ChAT, HB9) markers.

To verify the infectability of iPSC-MNs by SARS-CoV-2, VeroE6 cells were exposed to supernatants collected from iPSC-MNs infected with SARS-CoV-2. Reverse transcription polymerase chain reaction (rt-PCR) was used to analyze the expression of SARS-CoV-2-specific ORF7A, ORF3A, ORF8, RDRP, S, E, and N genes in uninfected (mock) and infected iPSC-MNs.

The expression of main SARS-CoV-2 receptors (ACE2, CD147, NRP1) and peptidases (TMPRSS2, furin) was assessed in iPSC-MNs infected with SARS-CoV-2 and the A549-hACE2 cells, as a positive control.

Results To validate the infectability of iPSC-MNs, VeroE6 cells were exposed to supernatants collected from infected iPSC-MNs at different time points. The results showed that supernatants collected from infected iPSC-MNs could re-infect VeroE6 cells. Furthermore, the SARS-CoV-2-specific genes (N, E, S, ORF3A, ORF8, and ORF7A) were detected exclusively in infected iPSC-MNs. The expression of SARS-CoV-2 N1, S1, S2, and E2 genes in infected iPSC-MNs was subsequently confirmed by rt-PCR.

The expression of SARS-CoV-2 specific N1 and N2 genes was identified in supernatants from infected iPSC-MN cultures by rt-PCR, confirming that SARS-CoV-2 can infect and replicate in human motor neurons. However, the viral replication level was lower in iPSC-MNs than in VeroE6 cells. In addition, SARS-CoV-2 replication in infected iPSC-MNs was not accompanied by a cytopathic effect as assessed by the crystal violet assay. In addition, the immunofluorescence assay detected N protein in infected iPSC-MNs, mainly at the perinuclear level, in the soma, and along the neurite extensions. However, the percentage of infected iPSC-MNs was very low.

Further analysis demonstrated that iPSC-MNs expressed the main entry receptors of SARS-CoV-2, including ACE2, CD147, NRP1, and TMPRSS2, but at different levels. ACE2 and furin were expressed at lower levels, whereas CD147 and TMPRSS2 were expressed at higher levels in infected iPSC-MNs compared to the control A549-hACE2 cell line. The NRP1 expression was comparable between iPSC-MNs and A549-hACE2 cells. The immunofluorescence assay for ACE2, CD147, and NRP1 confirmed these results.

In iPSC-MNs, SARS-CoV-2 infection changed the expression of 10 genes involved in cell survival, metabolism, antiviral, and inflammatory response. The virus up-regulated the expression of B-cell lymphoma-2 family protein (BCL2), BCL2-associated X protein (BAX), caspase 8, CD147, proinflammatory interleukin-6, and sphingosine-1-phosphate receptor 1, involved in the regulation of lymphocyte trafficking, brain and cardiac function, vascular permeability, and vascular and bronchial tone. The virus down-regulated the expression of human leukocyte antigen-A and endoplasmic reticulum aminopeptidase 1, involved in antigen processing and presentation, and angiogenin, which exerts neuroprotective functions and contributes to the systemic response to infection.

Interestingly, an increased ratio between the expression of anti-apoptotic BCL2 and pro-apoptotic BAX gene suggests that programmed cell death was somehow prevented in iPSC-MNs after the infection.

Conclusion The authors concluded that this study has shown, for the first time, that SARS-CoV-2 can infect and replicate in iPSC-derived human motor neurons. However, viral replication and the percentage of infected cells were lower than in VeroE6 cells, susceptible to SARS-CoV-2.

This article was published in Frontiers in Cellular Neuroscience.

Journal Reference Cappelletti G, Colombrita C, Limanaqi1 et al. Human motor neurons derived from induced pluripotent stem cells are susceptible to SARS-CoV-2 infection. Front. Cell. Neurosci, Sec. Cellular Neuropathology. 05 December 2023. Volume 17, 2023. (Open Access) doi.org/10.3389/fncel.2023.1285836 www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2023.1285836/full

[kristinahertzz]

Asthma and COPD Devices Market

Asthma and COPD Devices Market Size, Share, Trends: GlaxoSmithKline plc Leads

Rising adoption of smart inhalers and connected devices for better disease management

Market Overview: 

The Asthma and COPD Devices Market is projected to grow at a CAGR of 5.8% from 2024 to 2031. The market value is expected to increase significantly during this period.

North America currently dominates the market, with key metrics indicating a high prevalence of asthma and COPD in the region. The market is experiencing steady growth due to increasing awareness about respiratory diseases, technological advancements in inhaler devices, and a growing aging population prone to respiratory ailments.

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Market Trends: 

The asthma and COPD device market is shifting significantly towards smart inhalers and connected devices. These new technologies use sensors and mobile applications to monitor medicine use, send reminders, and collect data on patient adherence and lung function. This trend is being pushed by the growing emphasis on personalised therapy and the need for improved disease management. Smart inhalers provide real-time monitoring and data analysis, allowing healthcare providers to make more educated judgements and change treatment programs accordingly.

Market Segmentation: 

Inhalers dominate the asthma and COPD devices industry, accounting for over 70% of total sales. This dominance is due to the vast range of inhaler types available, such as metered-dose inhalers (MDIs), dry powder inhalers (DPIs), and soft mist inhalers (SMIs), which cater to various patient preferences and demands. Patients and healthcare providers choose inhalers because of their portability, convenience of use, and direct drug administration to the lungs.

Market Key Players:

GlaxoSmithKline plc

AstraZeneca plc

Boehringer Ingelheim International GmbH

Novartis AG

Teva Pharmaceutical Industries Ltd.

Contact Us:

Name: Hari Krishna

Email us: hari.krishna@aurorawaveintellects.com

Website: https://aurorawaveintellects.com/