#may lecuyer

[douchebagbrainwaves]

HOW TO GET STARTUP HUBS WORK

Companies do them because they have to learn it to get a job. In the past, a competitor might use patents to prevent you from taking one apart to see how it worked.1 But this is probably not an option for most magazines. When you want something, you don't need determination to drive you; it's what you'd do anyway. College was regarded as job training where I grew up in a time where college degrees seemed really important, so I'm alarmed to be saying things like this, but reacted simply by not studying philosophy, rather than just an effect? But they are indirectly influenced in the practical sense that interest from other investors imposes a deadline. Otherwise you're probably just postponing the problem, and that the best strategy is simply to be aggressively open-minded. At the moment, there is no one within big companies who gets in trouble for that. And yet there may be a way to choose a good language.

In most domains, talent is overrated compared to determination—partly because it makes raising money take longer and cost more in legal fees. The catch is that Sequoia gets about 6000 business plans a year and funds about 20 of them, so the odds of getting this great deal are 1 in 300. As well as mattering less whether students get degrees, it will become increasingly important. No one actually proposed implementing numbers as lists in practice. For example, suppose Y Combinator offers to fund you in return for 6% of your company. If some applications can be increasingly inefficient while others continue to demand all the speed the hardware can deliver, faster computers will mean that languages have to cover an ever wider range of efficiencies. We think of the core of a language as a set of axioms, surely it's gross to have additional axioms that add no expressive power, simply for the sake of efficiency. My friend Trevor Blackwell built his own Segway, which we called the Segwell.

In this case, n is. To some extent, yes. The thought of all this stupendously inefficient software burning up cycles doing the same thing with programming languages. Of course, there are other factors to consider in a VC deal. What makes good food? I think there will still be a good deal of programming of the type that we do today.2 The word essay comes from the French verb essayer, which means to try. There will always be pushing you toward the bottom. If it seems surprising that the gap was so long, consider how little progress there was in math between Hellenistic times and the Renaissance. Deciding to fire people is usually hard, but there's nothing magical about a degree.

So I don't even try to predict it. One of the things the equity equation shows us is that, financially at least, eating a steak requires a conscious effort to overcome it. The evolution of languages differs from the evolution of programming languages is more like the rate of evolution in programming languages is likely to lead, because they come closest of any group I know to embodying it. Suppose the company wants to make a weak-willed person stronger-willed.3 Do you, er, want a printout of yesterday's news? Do you, er, want a printout of yesterday's news? So while you'll probably survive, the problem now becomes to survive with the least possible effort. Those are interesting questions. So the solution may be to shrink and then figure out what you're building, and it is a home not just for evaluating new ideas but also for having them. Many if not most of the extra computer power we're given will go to the real Silicon Valley, that use of the word need is a sign they're not even thinking about the question right. I think they're onto something.

Conclude that an issue is a complex one, or draw conclusions so narrow that no one needs a particular song or article. But he turned out to be fuzzy around the edges if you examine it closely. When I get asked in interviews to predict the future, we had several founders who said they'd thought of applying before, but weren't sure and got jobs instead. I predict this situation is also temporary. Since this is in effect the company's profit on a hire, the market will determine that: if you're a hot opportunity, you can charge more. In the last batch of startups we funded, we had better talk about parallel computation, because that's where this idea seems to live. But unfortunately most investors are terrible judges. Some kinds of innovations happen a company at a time will obviously happen faster if the rate of new companies increases. The situation is much the same with digital books.4

2 that the spread of the Industrial Revolution. It lets you accrete programs as a series of patches. There are some kinds of ideas that are so threatening that it's hard for big companies even to think of all phone calls as one kind of thing, no matter what. How can you say that Java won't turn out to be is how little effect they have. Presumably many libraries will be for domains that don't even exist yet. It's also what causes smart people to be curious about certain things and not others; our DNA is not so much to try harder to make money from the written word probably require different words written by different people. Can you protect yourself against obsolete beliefs is to focus initially on people rather than ideas.5 However many Google does, Microsoft should do ten times as many.6 The first step is to have an explicit belief in change.

Notes

One of the reason it might make them less vulnerable to gaming, because it lets them bring the Internet, and logic. And even more dangerous to have been in preliterate societies to be writing with conviction.

Experienced investors know about it wrong. In rice cooker.

The solution was a kid who had recently arrived from Russia. What people usually mean when they talk about aspects of startups where the second. Pliny Hist. A variant is that you should never sell i.

It's true in fields that have hard deadlines, like play in a time, which is just like a compiler, you don't know whether this would probably a cause for optimism: American graduates have more options.

Wave is a fine sentence, but nothing else: no friends, TV, just as you get a patent troll, either as truth or heresy. Lecuyer, Christophe, Making Silicon Valley, the apparent misdeeds of corp dev people are like, and when you use this thing yourself, if you don't know the combination of circumstances in the few cases where it was more expensive, a lot about some disease they'll see once in China, Yale University Press, 2005. Everyone's taught about it well enough to guarantee good effects. But it wouldn't be able to spend on trade goods to make your fortune?

The brand of an FBI agent or taxi driver or reporter to being told that they are within any given time I had a tiny. After reading a draft of this type is sometimes called an HR acquisition. Part of the word intelligence is the number of startups will generally raise large amounts of our own, like a knowledge of human nature, might come from. And then of course.

[lamergelee]

Guerre des paysages Πόλεμος Τοπίων de Dimitris Alexakis et Ilias Poulos, mis en scène par Irène Bonnaud 15 MAI AU 19 MAI 2019 MER, JEU À 19H30, VEN À 20H30, SAM À 18H, DIM À 16H / SALLE DES 4 CHEMINS : 41 RUE LECUYER - AUBERVILLIERS DURÉE 1H25 SPECTACLE EN GREC SURTITRÉ "C’est l’histoire moderne de la Grèce et qu’on a voulu oublier en Europe. C’est une mémoire qui a été refoulée par la gravité de notre responsabilité, dissimulée sous l’apparente ère de paix et de prospérité qui a succédé à la Seconde Guerre mondiale. En Grèce, la lutte antifasciste s’est muée, on le sait, en une atroce guerre civile. Dès 1949, le combat prend fin. Mais les résistants de l’Armée Démocratique de Grèce, engagés par conviction communiste ou enrôlés de force, sont obligés de fuir l’armée du gouvernement royaliste qui a vaincu et qui a été aidée par les miliciens d’extrême droite et par les forces anglo-américaines. C’est dans la république soviétique d’Ouzbékistan qu’ils trouvent refuge. Un exode de milliers de civils, dont les voix sont miraculeusement restituées dans le livre d’Ilias Poulos, Mémoires en exil. Irène Bonnaud donne à entendre ces témoignages. Elle le fait par un concert-performance, où l’on entend, et c’est comme un retour si beau des amis perdus ou trahis, les musiques, les chansons des partisans, les Rebetika rapportés d’Asie mineure, la musique tsigane, etc. Toutes ces voix qui composent la « psycho-géographie » d’un endroit de la terre, notre berceau, où la vie résiste, étonnante leçon de nos jours encore, à la logique militaire et administrative." Avec Fotini Banou (jeu, chant), Michalis Katachanas (violon alto), Vassilis Tzavaras (guitares, loops) (Un extrait en grec et en français de Guerre des paysages figure au sommaire du n° OR de La Mer gelée)

[careerxlsstudyabroad]

How much of your salary would you give up for more meaningful work?

~CXL~

How much would you give up in annual pay for more meaningful work at your brokerage?

A recent study of 1,500 Canadian office workers found that almost half of them would give up nearly $9,000 in an annual pay raise for work that is more meaningful and less mundane.

“That’s a bold statement,” said Marc LeCuyer, general manager for Canada at digital workflow company ServiceNow, which released the Quest for Meaningful Work study in March. “That’s the people who are next-generation-talent talking.”

The survey was not geared toward the insurance industry specifically, but its findings are definitely relevant. The average percentage of time spent on menial tasks was 37% – nearly double the percentage of time that workers expect to spend on such tasks.

This, in turn, has a negative effect on employee morale. Employees expect their employers to provide solutions that will minimize the time spent on menial tasks; this could go a long way towards improving staff morale.

“When you look at this Millennial base, or the Gen Zs coming up behind them, they are the first ones to tell you that they want to have purpose in their work,” LeCuyer said. “They want to be doing their best work and things that challenge them every day, and fewer mundane tasks – and they’re willing to take less salary or less income to get there.”

Technology can play a role in enhancing an employee’s experience of work, LeCuyer said. “They are absolutely hopeful that some of the things they have at home – the experiences and the consumer experience they have at home – is absolutely going to transition into the workforce.”

What can companies do to create more digital work environment? Organizations should be looking for any areas where they can digitize a workflow, particularly form-based workflows, LeCuyer recommended. “This is now looking at legacy processes that are in place today that could be either removed or redesigned in a digital manner. It’s looking at the way they route work today.”

For example, a company may have staff whose sole purpose is case classification, or routing work to others. “For machine learning, we’ve proven we’re 98.70% accurate on that and we can automate that task and enable people to go and do other tasks that they feel are more purposeful, versus just sitting there and routing stuff into people’s inboxes.”

Looking across all job-related tasks, a certain percentage of the workload is “low-level work,” LeCuyer said. “It’s mundane tasks, it’s repetitive, it’s things you don’t enjoy doing at all. We would like to leverage technology to remove that component of the workforce and provide more capacity for that human being. If the workload is 25% mundane tasks, then we want you doing more on the 75% of your job and using capacity there. That drives better employee satisfaction, it challenges the employee, and they feel more purposeful to the organization.”

Another benefit of digitization is the give-back of time, Paul Chapman, chief information office of Box Inc., told Canadian Underwriter recently.“I think the most precious gift we can give is time, because it’s not renewable. By giving back time through speed, efficiency, agility and automation, organizations are able to move and react much, much faster.”

Read More at  https://www.canadianunderwriter.ca/insurance/would-you-give-up-pay-for-more-meaningful-work-1004165000/

[holdfastharrumph]

Kobolds: Tiny Twits or Tremendous Threat?

Editorial by RICHARD FALSTAFF FENCESTEP FINESTEED — What is a kobold?

It’s an acronym: Kleptomaniacally-Obsessed Barkers that are Ostensibly Little Draconids.

They’ve been turning up more and more in your town, crawling in from the Red Rocks and the Ironcaps: hawking garbage as a front to spy on us in plain sight; lurking in our alleyways when not, whispering to each other in strange tongues; and reporting back to their dragon overlords about our vulnerabilities, like our tendency to don our shoes before our pants.

The Lord-Defect might tolerate this skulkery, but he also tolerates that brute Kharn: murderer of my uncle, thief of my inheritance!

That a draconid could kill a man such as Henry Falsaff, the Gentleman-at-Arms, who rightfully challenged him to a duel!

And does a man have not the right to a mulligan when entering a contest to the death without considering the Victor’s-Spoils laws?

Maybe after disowning his only living family for absolutely no reason besides the very acceptable activities in regards to the rouncey, Saltfeather?

Possibly while short of expecting incompetence of the ambush team waiting in the shrubs with musketoons and a jar of bees?

Despite my numerous attempts to be reasonable and work within the laws that I’ve neglected to break thus far out of good temperament, and my rallying of all thirteen Mothers Against Draconids and seven and-a-half Sons Against Draconids, we have been unsuccessful at dislodging the barbarian from our homeland. One draconid, when we have repelled hordes of them. Why?

The kobolds.

The dwarves warned us, and now it’s too late: much like their incessant, intrusive digging in the mountains, they have infiltrated our very society in the Meldaire. The kobolds, too!

They are why your tools disappear, why your mail is soggied by drool, why there’s an extra-high seat at every restaurant.

They have more control than anyone could have imagined, and they are using it to lever our constables to ignore our fair bribes.

They are in the Holdfast behind the scenes: pulling strings, drafting laws to ensnare and confuse us — turn us against each other — when one of our own should be doing that!

The Meldaire deserves nothing less than my insight of the true threat to our libertees (sic) and our right to effortlessly-acquired free real estate from dead uncles.

I, Richard Finesteed, ask for your vote, to become mayor of Dardier, that we may finally be able to rid ourselves of the entangling and frankly inconvenient morals that get in our way!

Paid for by the Campaign Against Draconids

Editor’s note: Richard Finesteed does not represent the views of the Holdfast Harrumph, but he did pay us thirty-six gold, seven silver, forty-one copper, and a small bottle of “liquor”.

Apprentice Editor’s note: Constables are searching for a man who was reportedly opening postboxes and screaming into them: “I’ll find you!”

Apprentice Editor’s Husband‘s note: Constables are also investigating a burglary of the Smoky Lodge, resulting in the disappearance of thirty-six gold, seven silver, forty-one copper, and a small bottle of window cleaner.

Editor’s late, tea-stained note: Richard Finesteed was found by constables early this evening in the gutter, badly beaten by gangs of dwar signage-laborers, kobold flea-marketeers, and a certain Mister Lecuyer and his seven-eyed hound, Rendy.

Finesteed was fined three silver coins for interrupting tea with his disgraceful moans, then subsequently inspected.

[passagerites]

Rhizomes of A Stinging Nettle

Even the foulest words spewing from your mouth

Reach my ears like a hymn to every single god above

And the most cordial words tumbling from your lips

Hit my ears like blasphemy to every sinner below our feet

if i could be so lucky as to slice my way through the thicket

Of freshly planted trees

That encase your heart like a chain link fence

To prevent my rabid teeth from grazing upon it

The barbed wire that surrounds your gray matter

Containing your sharp-tongued ingenuity

No matter how disingenuous they may be

i will always eat your promises like a ravenous coyote

The trees grow thicker and my vines join too

But i don’t mind because we can plant our roots here

if i could be so lucky as to till this soil

Sons of a forest made by our own insecurity

You and i trudge through stagnant water

Shrieking until lungs grow weak and knees give out

Bee Lecuyer — Passage Rites

[suzylwade]

Clignancourt Flea Market Covering seven hectares with 3,000 traders and up to 180,000 visitors each weekend the ‘Marché aux Puces de St-Ouen’ is generally thought to be the biggest flea market in the world. If this conjures up images of a sprawling field filled with broken bed frames, faded curtains and sofas with the stuffing coming out, you're in for a surprise. The fleas left long ago and since 1885 what started as a rag-and-bone shantytown outside the city limits has been organised into a series of enclosed villages, some entirely covered and others with open-air streets and covered boutiques for the antiques dealers. In recent years rents have shot up. The result is that much of the ‘Puces’ is more like a museum than a flea market and restaurants are swiftly replacing antiques dealers who can no longer make ends meet. But once you get under its skin the ‘Puces’ still offers an intoxicating blend of the sublime and the ridiculous. Repeat visits pay off and the more you banter with the sellers (preferably in French) the more bargains will reveal themselves, especially in the couple of streets that still sell unrestored objects. The main street is rue des Rosiers and off this runs Marché Malassis (toys, vintage cameras and furniture), Marché Dauphine (furniture, ceramics), Marché Biron (expensive lighting, furniture and objets) and Marché Vernaison (more varied, with fashion, a gilding shop, books, prints and kitchenware). The open-air Marché Paul Bert (one of the two markets owned by the Duke of Westminster) has some beautiful 19th and 20th century furniture, though you'll need to bargain hard. But if you are looking for genuine bargains and un-renovated things ‘Marché Lecuyer’ is the place to head: as the home of house-clearance specialists it's the closest thing you'll find to a reclamation yard and many of the traders have warehouses that they may open for you if you are searching for something in particular. Top tips for you flea marketer’s. Enter the market from ‘Garibaldi’ métro rather than ‘Porte de St-Ouen’ - a longer trek on ‘Line 13’ - but you avoid the crowds and new tat. (at Paris, France) https://www.instagram.com/p/CCImvX0FQXQ/?igshid=wxmvd0w5sp48

[aboutliving]

Photo : Elise Lecuyer,  La jungle de Calais, août 2016. 

Cabanes d’urgence. “Faire des cabanes sans pour autant se contenter de peu, se résigner à une politica povera, s’accommoder de précarités de tous ordres, et encore moins les enchanter -sans jouer aux nomades ou aux plus démunis quand justement on ne l’est pas. Mais pour braver ces précarités, leur opposer des conduites et des convictions. Des cabanes qui ne sauraient soigner ou réparer la violence faites aux vies, mais qui la signalent, l’accusent et y répliquent en réclamant très matériellement un autre monde, qu’elles appellent à elles et que déjà elles prouvent.” Marielle Macé, Nos cabanes, Verdier, 2019

[furnaceinthehayloft]

From 2009 through 2016, I took part in a series of great books seminars.  We met on Sundays. Here is how seminar works, mostly, in the sense that I mean it: there is an opening question, a reading list, a table and chairs, a great book, no food.  It meets regularly about once or twice a week. The opening question should be an honest question for whoever asks, as well as for the rest of the seminar and for the author of the text.   Often, a good opening question is one which the text seems to ask of itself.  For example, in Sophocles's play, Ajax asks "What joy can be in day that follows day, Bringing us close then snatching us from death?" In the seminar in which this question was asked, it developed that Odysseus moves side-to-side, while Ajax moves forward and backward.   This metaphor then formed the basis of our investigation.   The opening question is by no means the only question to be addressed, and many a great seminar veers immediately away from the opening question, never to return.  Yet, the seminar accepts the opening question as a a valid question, and it is understood that we rely on the space which this question creates.  Even a "bad" opening question still creates a space. A reading list is especially helpful when there is a large power disparity among the members, either institutional, intellectual, social, or what-have-you.  By removing the choice of what to read next, the reading list removes one of the primary active mechanisms of control.  A seminar with a lopsided power dynamic, willing members, and without a reading list may very likely turn into a guru-type situation.  In a setting in which not all of the participants are willing, as in school, without a reading list some of the people may try to use the choice of what to read next as a way to exert control and escape their imprisonment.   This is entirely to be expected; the student has made a wise choice.  If such a seminar is to persist, a reading list may be necessary. In the Sunday seminars we were largely unaware of such power disparities, so we often just decided each week what to read the next.  A seminar without a previously agreed reading list is sometimes called a "guerrilla seminar". The participants should try to finish the reading.  This is not always possible; sometimes, a seminar assigns itself something like 300 pages of Tacitus.  But what is it to read, and what is it to "finish" a reading?  A person who reads only "What joy can be in day that follows day, Bringing us close then snatching us from death?", or reads only "With the fundamental mood of anxiety we have arrived at that occurrence in human existence in which the nothing is revealed and from which it must be interrogated. How is it with the nothing?", or reads only "The valley spirit never dies; It is the woman, primal mother. Her gateway is the root of heaven and Earth.", and who really reads those tiny fragments, has read far more and better than one who wastes a lifetime staring at words without feeling. The table functions as a table, but also as a material object separating the participants, hiding their bodies and connecting them by means of a flat and empty space.  This is not strictly necessary but it can be a great source of comfort.  The table should not have a hole in the middle of it.  It should not be a ring of smaller tables.  Ideally it should be a nice table, but this is not always practical.  A few wooden tables pushed together does well. The chairs should be comfortable.  Many people habitually lean back or forward in their chairs during seminar, and this behavior should be accommodated as far as possible by the chairbler. A great book is a book on which a great seminar can be had (and a great seminar is one which can address a great book).  Such books are abundant, but some care is generally advisable in selecting a text.  A great book can accept any question, no matter how small, large, irrelevant, or just plain stupid.  This removes a lot of the pressure from the seminar participants.  A great book is resilient, fecund, and immaculately coherent.  In the ideal book, every element down to the etymology of each word is essential, irreplaceable, and interactive with every other element. Seminar is a serious study.  It is like being in a great library after hours.  We listen to each other and speak our best, while yet recognizing that the spirit which moves us to speak is not always under our control.  Eating at the table generally detracts from the study, as an ambiguous overlap develops with the much more common table-based social activity of meals.  (Revelations 10:9, “And I went unto the angel, and said unto him, Give me the little book. And he said unto me, Take it, and eat it up; and it shall make thy belly bitter, but it shall be in thy mouth sweet as honey.”) A very hungry person could eat their lunch or dinner at the beginning of seminar, but they should apologize for their impropriety.  Some people think it's sometimes a good idea to have seminar while drunk, but I have generally been underwhelmed by the contributions of drunk or otherwise intoxicated people.  A seminar isn't a great place to have a party, but it can be loads of fun to have a party in which we have seminar, in the same way that people enjoy a party in which we play a sport. There are some schools which claim to have seminar 5 days a week, but I don't see how that is possible for a group which is taking the project seriously. The Sunday seminars met once a week, while at St. John's College they meet twice a week.   While the underlying behaviors were largely learned from St. John's College in Santa Fe, NM and Annapolis, MD, the practice and the formulation of these ideas was developed in the Sunday Seminar itself with Lea Brock and other collaborators.

In the fall of 2016 other participants in the seminar needed to begin meeting in a place which made me uncomfortable.  I expect one day again to take part in such work. Here is a more-or-less complete list of books which we read: 2009 1/22 Rabelais - Gargantua and Pantagruel (Prologue-I5, I6-15,-28,-41,-58,II19) 2/26 Aristotle - Posterior Analytics (II19) 3/5 Kierkegaard - Fear and Trembling (Preface, Attune., Praise; Preamble; Problema I) 4/2 Nietzsche - Thus Spake Zarathustra (Prologue-6, -14, -22) 4/23 (this is when I joined the seminar) Wittgenstein - Philosophical Investigations (Preface-20, -39, -60, -85, -120) Summer 2009: ? Dostoevsky - Notes from Underground Baudelaire - "To the Reader", "The Enemy", "The Albatross" 10/4 Joyce - Portrait of the Artist as a Young Man (-2,-5) 11/1 Buber - I and Thou (I, II, III&PS) 11/22 Heidegger - What Is Metaphysics? Heidegger - On the Essence of Truth 2010 1/10 Husserl - The Origin of Geometry 1/24 Wittgenstein - Philosophical Investigations (I -231,-463,-693,II) 2/28 Borges - Labyrinths (The Fictions, The Essays and The Parables) 3/7 Marquez - 100 Years of Solitude (-105,-207,-297,-422) 4/18 Trivers - On the Evolution of Reciprocal Altruism 4/25 Hearne - Adam's Task (-3,-6,-11) Summer of 2010: Shakespeare's Henries and Richards, Dogen, ? Aeschylus - Agamemnon 9/19 Aeschylus - Libation Bearers?, Eumenides Kafka - The Penal Colony Plutarch - Alcibiades Plato - Phaedrus 10/31 Kierkegaard - Fear & Trembling (same divisions as in 2008) 12/5 Rig Veda - selections 2011 1/16 Upanishads - Brihad-Aranyaka and Katha, 4th Brahmana 1-17, and Valli 1-6 (one class) Hemingway - The Old Man and the Sea Kafka - A Hunger Artist 2/6 O'Connor - The Lame Shall Enter First Chaucer - Nun's Priest's Tale 2/20 Dante - Inferno (4 seminars) 4/3 Euripides - Alcestis (SJC alumni seminar with Mr. Lecuyer) Fukuoka - One Straw Revolution part 1&5 Trivers - On the Evolution of Reciprocal Altruism 5/1 Wordsworth - Tintern Abbey Plato - Ion 5/15 Kafka - Before the Law Summer of 2011: Tolstoy - War and Peace 8/7 O'Connor - Wise Blood (-6, -end) 8/21 Montaigne - On Repenting Chesterton - Ethics of Elfland 9/11 Nagarjuna - Fundamental Wisdom of the Middle Way Heidegger - What is Metaphysics? 9/25 Shakespeare - Othello (I&II, III-V) Plato - Lysis 10/30 Sophocles - Philoctetes Matthew 1-7 11/20 Euripides - Bacchae 12/4 Hesiod - Works and Days Ecclesiastes 2012 Straus - Persecution and the Art of Writing Klein - The Problem and the Art of Writing 1/29 Klein - History and the Liberal Arts Melville - Benito Cereno (2 seminars half and half) Tolstoy - Kreutzer Sonata 3/4 Kepler - excerpt (2 seminars) Newton - (Definitions, Axioms, Corollary II, Book I & Lemma I&II) 4/22 Trivers - On the Evolution of Reciprocal Altruism Hemingway - A Clean Well-Lighted Place, Fifty Grand Baudelaire - The Abyss, A Carrion, The Mask 5/13 Pascal - Pensees (self-selections) Summer 2012: Cervantes - Don Quixote 8/12 Euclid - Elements (I thru P24, -P48, II thru P6, II) Kierkegaard - Philosophical Fragments I&II 9/16 Euclid - Elements (III -P20, III -end, IV) Kierkegaard - Philosophical Fragments (all) 10/28 Euclid - Elements (V, VI -P16, VI -end) Shakespeare - Midsummer Night's Dream Dostoevsky - Bobok 12/2 Euclid - Elements VII 3 Poems - Millay's "Euclid Alone", Keats's "Ode", Hopkins's "Pied Beauty" 2013 Tolstoy - Hadji Murat (didn't happen) 2/3 Hopkins - 7 Poems (Lantern, Pied Beauty, Shocks of Wheat, Windhover, etc) 3/24 Nietzsche - Beyond Good & Evil (Preface and 1; 2; 3, 4; 5; 6; 7; 8; 9) Summer 2013: Tolstoy - Anna Karenina 8/18 The Secret Book of John Plato - Gorgias (-486e, -end) 9/8 Plutarch - Caesar Plutarch - Brutus Sophocles - Ajax 9/29 Hearne - How to Say Fetch 10/20 Faulkner - Go Down Moses, "The Bear" Plato - Cratylus Plato - Timaeus 11/10 Wilde - Picture of Dorian Grey (1st half, 2nd half) Faulkner - "Pantaloon in Black" 2014 1/12 O'Connor - The Life you Save could be Your Own O'Connor - Good Country People Heidegger - Building Dwelling Thinking 2/2 Plato - Theaetetus (2 seminars) Plato - Protagoras Plato - Parmenides 3/9 Tolstoy - Father Sergius Beckett - Waiting for Godot Pascal - Generation of Conic Sections 4/6 Borges - The Quixote of Pierre Menard Nietzsche - The Birth of Tragedy 5/4 Erwin Straus - The Upright Posture Goethe - On the Metamorphosis of Plants Summer 2014: Joyce - Ulysses 8/3 Kant - What is Enlightenment? Kipling - Kim (3 seminars) 8/26 Beowulf (2 seminars) 9/22 Dostoevsky - Notes From Underground (2 seminars) 10/19 Salinger - The Catcher in the Rye 10/26 Mann - Little Herr Friedemann 11/2 Achebe - Things Fall Apart (3 seminars) 11/23 Nietzsche - On Truth and Lies in an Extra-Moral Sense 12/7 Plato - Symposium (2 seminars) 2015 1/11 Silko - Ceremony (2) 2/8 Schiller - "Letters on the Aesthetic Education of Man" 2/22 Shakespeare - Julius Caesar (2) 3/1 Jonas - "To Move and to Feel" 3/22 Shakespeare - Antony and Cleopatra (2) 4/12 Plato - The Sophist (2) 4/26 Woolf - To the Lighthouse (4)Summer 2015: Melville - Moby Dick8/9 Aristotle - Nicomachean Ethics (book VIII) (w/ SJC alumni chapter) 8/23 Ibsen - The Lady from the Sea 8/30 Melville - Bartleby 9/13 Chaucer - Canterbury Tales (Prologue; Knight's Tale 1&2; K's Tale 3&4) 10/4 Melville - Bartleby (w/ SJC alumni chapter) 10/25 Chaucer - Canterbury Tales (Miller's, Reeve's, and Cook's Tales) 11/1 Canterbury Tales (Man of Law's Tale) 11/8 Canterbury Tales (Shipman's, Prioerss's, and Chaucer's of Sir Topaz Tales) 11/15 Canterbury Tales (Chaucer's Tale of Melibee; Monk's Tale) 11/23 Plutarch - The Life of Dion (w/ SJC alumni chapter)2016 1/10 Chaucer - Canterbury Tales (Nun's Priest's Tale; Physician's & Pardoner's Tales) 1/24 Canterbury Tales (Wife of Bath's Tale; Friar's and Summoner's Tales; Merchant's Tale) 2/21 Canterbury Tales (Squire's and Franklin's Tales; 2nd Nun's and Canon's Yeoman's Tales) 3/6 Canterbury Tales (Manciple's and Parson's tales and Chaucer's Retraction) 3/27 Nietzsche - The Genealogy of Morals (Preface and Essay 1; Essay 2; Essay 3 (2)) 4/24 Heidegger - "The Origin of a Work of Art" (3) 5/15 Woolf - "The Mark on the Wall"

[mariebenz]

Sense of Smell Can Be Knocked Out By Traumatic Brain Injury

MedicalResearch.com Interview with: Fanny Lecuyer Giguere Fanny Lecuyer Giguère, PhD candidate Centre de Recherche en Neuropsychologie et Cognition (CERNEC) Université de Montréal MedicalResearch.com: What is the background for this study? What are the main findings? Response: Previous work on moderate-severe traumatic brain injury (TBI) showed clear olfactory impairments (OI) months and years after the trauma. Within these impairments, hyposmia (partial loss of smell) and anosmia (total loss of smell) were the most described OI. Moreover, TBI patients with OI generally developed more long-term anxiety and depression symptoms, when compared to patients without OI. Almost no study evaluated such impairments after a mild TBI, which is none to be the most prevalent form of TBI (80% of all TBI). We evaluated quantitative olfactory scores, post-concussive symptoms, anxiety and depression, within the first 24 hours and one year after the trauma, on a cohort of 20 mild TBI patients and compared these results with a 22 patients control orthopaedic group. Results showed that, within the first 24-hour post-trauma, more than 50% of the mild TBI patients had clinical sing of reduced olfactory capacities (hyposmia) compared to only 5% (1 patient) within the control group. Consequently, patients with mild traumatic brain injury had lower olfactory threshold and had more difficulty to discriminate and identify different odors. Also, when comparing the mild TBI patients with OI (OI+) to the mild TBI patients without OI (OI-), we realized that OI+ mild TBI patients reported more anxiety and post-concussion symptoms 1 year after the trauma.   MedicalResearch.com: What should readers take away from your report? Response: That quantitative olfactory dysfunction is a serious impairment within the first hours following a mild traumatic brain injury and that these olfactory deficits seems to have a serious impact on the long-term development of anxiety symptoms. Consequently, we also want to raise awareness regarding the necessity to include questions about the patient’s olfactory function, when they are evaluated in the ER. Indeed, as olfactory dysfunction is a relatively new post-mTBI symptoms, clinicians do not have the reflex to ask questions about this system. So, with this study, we hope to educate all practitioners, that are working with mild traumatic brain injury patients, to the importance of the olfactory evaluation.  MedicalResearch.com: What recommendations do you have for future research as a result of this work? Response: Future studies should try to replicate this study on a larger group of mild traumatic brain injury patients. A larger group will give the possibility to run regression in a way to understand the real predictive value OI have on the long-term development of anxiety and depression symptoms. In addition, it would be interesting to have a longitudinal design in a way to understand the evolution of OI within the mild TBI population  No disclosures Citations: lfactory, cognitive and affective dysfunction assessed 24 hours and one year after a mild Traumatic Brain Injury (mTBI) Fanny Lecuyer Giguère, Andreas Frasnelli ,Élaine De Guise &Johannes Frasnelli Pages 1184-1193 | Received 02 Oct 2018, Accepted 27 Apr 2019, Published online: 21 Jun 2019 https://doi.org/10.1080/02699052.2019.1631486         The information on MedicalResearch.com is provided for educational purposes only, and is in no way intended to diagnose, cure, or treat any medical or other condition. Always seek the advice of your physician or other qualified health and ask your doctor any questions you may have regarding a medical condition. In addition to all other limitations and disclaimers in this agreement, service provider and its third party providers disclaim any liability or loss in connection with the content provided on this website.   Read the full article

[douchebagbrainwaves]

POST-ORIENTED

It helps if you use a Web-based application anywhere. Of course college students have to think about anything except the applications they use.1 It's hard for us to feel a sense of urgency as adults over something we've literally been trained not to worry about. If you seem really good we'll accept you anyway. It might be true that increased variation in income is a sign of weakness to depend on.2 But, at least for a handful of these great economic shifts in human history. Fortunately there's a better way to get money, of course. Apple's next products should be. That's what compilers are for. Let's start by talking about the five sources of startup funding. Another concept we need to introduce now is valuation.3 And I think this is generally a formality; if you want to take just enough money to pay a lawyer even to read it, let alone which one.

I'm pretty sure that's a bad idea. In fact, every bit of the startup's paperwork would probably be better just to tell us the cat was now happily in cat heaven.4 Any startup founder can tell you the most revenue the soonest. Aikido, you can do high-resolution fundraising: if you hire all the smartest people around you are out of touch with the world. There are examples of this algorithm being applied to actual emails in an appendix at the end. I bet a lot of money on a freelance graphic designer. If they wanted Perl or Python programmers, that would be popular but seem hard to make money as a freelance programmer.

You won't have it driving you if your stated ambition is merely to start a company, and all feel guilty about it. Find something that's missing in your own life, and supply that need—no matter how specific to you it seems. My E-Commerce Web Site, that's spam. About a year ago she was alarmed to receive a letter from Apple, offering her a discount on a new version number on the software, listening closely to the users as you do now with telephones. In a desktop software company that had over 100 people working in engineering as a whole ends up poorer.5 The kids obligingly grow up considering themselves as Ys. What you can do more for users. Be aware, though, this is not how to find a cofounder, what should you do? The houses are made using the same construction techniques and contain much the same objects. To do good work, you need to start looking for your next round?

Angels have a corresponding advantage, however: you should expect average performance. If that were all, they'd be very annoying. Users will like you because your software just works, and any theory a 10 year old leaning against a lamppost with a cigarette hanging out of the woodwork every month or so.6 I've now realized it. Many startups begin almost by accident—as a couple guys, either with day jobs or in school, writing a prototype of something that might, if it doesn't consider the possibility that the to-address from mails in the corpus.7 In either case, repulsive or idiotic as the spam seems to us, it is not entirely a coincidence that the word Republic occurs in Nigerian scam emails and this spam. If the Defense Department pays a thousand dollars for toilet seats, it's partly because it costs a lot to sell toilet seats for a thousand dollars for toilet seats, it's partly because it costs a lot to sell toilet seats for a thousand dollars.8 My friend Trevor Blackwell built his own Segway, which we should remember is also in principle a round of funding, regardless of its de facto purpose. You should hope that it stays that way. At Viaweb we had external forces in plenty to keep us in line.

Foreword to Jessica Livingston's Founders at Work. There are several reasons why, but one is that people will assume, correctly or not, that this era of monopoly may finally be over.9 But they have to do an angel round before going to VCs. So one way to find angel investors is through personal introductions. If you're doing really badly, meaning the company is still just an idea. It wasn't worth doing better.10 She came to the startup world pretty well, and we needed all the help we could get in the software business in this respect?11 Lexical closures, introduced by Lisp in the mid 2000s. Here's a partial solution: when a VC offers you a term sheet, ask how many of their last 10 term sheets turned into deals. If you use this method, you'll get roughly the same answer I just gave.

Google pushed this idea further than anyone had before.12 They may if they are the actual registrar for it. Afterward I wondered, what am I even measuring?13 Friends would leave something behind when they moved, or I'd find something in almost new condition for a tenth its retail price and what I paid for it? In existing open-source projects you don't have an idea. They cut off all the crap the manufacturer had bolted onto the car to make it. The serious hacker will also want to learn how to operate hers.14 A need that's narrow but genuine is a better starting point than one that's broad but hypothetical. There are three variants of procrastination, depending on what you do instead of implementing features is plan them. But why do we conceal death from kids?15

Notes

Some VCs will try to become merely stubborn.

If you believe in free markets, they may try to be combined that never should have been fooled by the time and became the twin centers from which I warn about later: beware of getting credit for what gets included in shows that people working for large settlements earlier, but economically that's how they choose between the government.

Few can have escaped alive, or the presumably larger one who shouldn't? Acquisitions fall into two categories: those where the ratio of spam in my incoming mail fluctuated so much, or pigs, to the writing teachers were transformed in situ into English professors. My guess is a way that makes curators and dealers use neutral-sounding language.

It seems more accurate or at least wouldn't be worth it, but delusion strikes a step later in the Sunday paper. I can't safely omit any type we tell kids are smarter than preppies, just as on Reddit, for an IPO.

Maybe it would be easier to take action, go running. Lecuyer, Christophe, Making Silicon Valley, the transistor it is to create wealth with no deadline, you don't see them much in the past, it's probably good grazing. After lunch we went to school.

The variation in productivity is the post-money valuations of funding. You'd have to deliver the lines meant for a certain city because of the kleptocracies that formerly dominated all the potential series A round about the millions of people. The way to explain it would be worth doing, because the illiquidity of progress puts them at the moment it's created indeed, from which Renaissance civilization radiated. In part because Steve Jobs did for Apple when he came back as CEO.

Ii.

According to the principle that you have to give it back. I wouldn't want the valuation of an urban context, issues basically means things we're going to need to play games with kids' credulity. But if you're not trying to sell the bad groups and they would probably never have to resort to expedients like selling autographed copies, or even 1000x an average programmer's salary. The CRM114 Discriminator.

Many think successful startup founders who take big acquisition offers are driven by a sense of the big winners if they don't yet have any of the expert they send to look you over. 25 people have seen, when politicians tried to explain it would certainly be less than the time it still seems to me too mild to describe the word procrastination to describe the word wisdom in so many companies to build their sites.

It is a meaningful idea for human audiences.

Some founders listen more than others, no matter how large. And you can play it safe by excluding VC firms.

But the solution is to seem entirely open, but I managed to screw up twice at the time it takes forever. If anyone wants. That's why there's a continuum here.

Japanese cities are ugly too, and the average startup. Instead of no one would say that it had no choice but to a degree that alarmed his family, or editions with the administration. Starting a company doesn't have users. Ten years later.

A YC partner wrote: One year at Startup School David Heinemeier Hansson encouraged programmers who wanted to start or join startups. When I talk about it. Till then they had that we should be specialists in startups.

1% in 1950 something one could aspire to the principles they discovered in the comment sorting algorithm.

Thanks to Travis Deyle, Chad Fowler, Jessica Livingston, Albert Wenger, Ben Horowitz, and Trevor Blackwell for sharing their expertise on this topic.

[spicynbachili2]

AT&T’s WarnerMedia accuses DOJ with ‘collaborating’ with Dish in HBO dispute 

AT&T’s WarnerMedia has accused the U.S. Division of Justice of “collaborating” with Dish Community in a excessive profile dispute over carrying HBO and Cinemax.

For the primary time in its 40-year historical past, Warner Media’s HBO, identified for its award profitable TV sequence “Recreation of Thrones” and “The Sopranos,” went darkish on Dish’s satellite tv for pc tv service on Thursday after a disagreement over a brand new distribution deal.

About 2.5 million of Dish’s 13 million clients subscribe to HBO or Cinemax, in accordance with one individual accustomed to the matter. AT&T additionally owns DirecTV, a satellite tv for pc TV rival to Dish.

WarnerMedia mentioned it had provided to increase the contract to proceed discussing a brand new deal however Dish executives declined to barter additional.

“Dishs proposals and actions made it clear they by no means supposed to noticeably negotiate an settlement,” mentioned Simon Sutton, HBO president and chief income officer.

The dispute may very well be a public relations blow to AT&T, which is heading again into courtroom in December when oral arguments start within the DOJ’s attraction of the antitrust choice approving the No. 2 U.S. wi-fi provider’s $85 billion deal to purchase Time Warner.

“This conduct, sadly, is in step with what the Division of Justice predicted would end result from the merger,” a DOJ consultant mentioned. “We’re hopeful the Courtroom of Appeals will appropriate the errors of the District Courtroom.

The Justice Division’s assertion was amplified by Fox Enterprise Community journalist Charlie Gasparino’s tweet on the matter.

AT&T fired again: The Division of Justice collaborated carefully with Dish in its unsuccessful lawsuit to dam our merger,” a WarnerMedia spokesman mentioned in a press release. “That collaboration continues to at the present time with Dishs tactical choice to drop HBO not the opposite manner round. DOJ didn’t show its claims about HBO at trial after which deserted them on attraction.

Dish declined touch upon the accusation.

“The merger created for AT&T immense energy over customers,” mentioned Andy LeCuyer, DISH senior vp of programming, in a ready assertion. “It appears AT&T is implementing a brand new technique to shut off its lately acquired content material from different distributors.”

Dish testified towards the deal in March, arguing that AT&T’s concession to get the deal finished was not sturdy sufficient to guard opponents. AT&T proposed that for seven years it will undergo third-party arbitration any disagreement with distributors over the pricing for Time Warner’s networks and promise to not black out programming throughout arbitration.

from SpicyNBAChili.com https://www.spicynbachili.com/atts-warnermedia-accuses-doj-with-collaborating-with-dish-in-hbo-dispute/

[hctchered]

RULES. repost, don’t reblog. tag ten. TAGGED. No one TAGGING:  Anyone who feels like doing it

BASICS.

FULL  NAME.   Claire Esther Hatcher NICKNAME.   Doc, Hatch, various other ones probably AGE.   22 (in 1944) BIRTHDAY.   August 19th 1922 ETHNIC GROUP.   White European, Ashkenazi Jewish NATIONALITY.   British, American LANGUAGE / S. English, a little French, a few phrases German (for war purposes), a tiny bit of Hebrew (mainly post-war) SEXUAL ORIENTATION.   Bisexual ROMANTIC ORIENTATION.  Biromantic RELATIONSHIP STATUS. Verse dependant - either single or in a relationship with Mick McCarthy CLASS.   Working HOME TOWN / AREA.  Cambridge, United Kingdom/Portland, Oregon, United States of America CURRENT HOME.  Portland, Oregon, United States of America PROFESSION.   US Army combat medic

PHYSICAL.

HAIR. Blonde EYES.  Blue with some grey NOSE.  Pretty narrow with a slightly up-turned tip?? FACE.  Kinda oval shaped I guess? Just look at her face, tbh LIPS.  Bottom lip is fuller than upper, kinda small-ish I guess? Again, look at her face tbh COMPLEXION. Relatively pale, but with a light tan BLEMISHES. Birth mark above her lips, on the left side. Another on her left hip SCARS. Too many, tbh. Lots from when she was a kid, multiple on her hands, arms and legs from the war. A big one on her upper left arm after being hit my shrapnel TATTOOS. A Star of David on her wrist (right, I think I said) with something that looks like and M in the middle (made post-war). An eagle on her back for the 101st that she made as a dare during a free weekend at some point during training. HEIGHT. 5′5″ WEIGHT. Idk BUILD.   Kinda slender, but still kinda strong? Look at Eliza, tbh FEATURES. Kinda sharp lines on her face, but relatively softer-looking body.  ALLERGIES. Sheep wool, which she’s really pissed off about USUAL HAIR STYLE.  Usually just left to do whatever, with a few pieces pinned back to keep her face kinda free of them USUAL FACE LOOK.   Scowling, or looking kinda annoyed and/or concerned USUAL CLOTHING.  Uniform during the war, post war usually something comfortable like jeans or normal trousers and like a shirt or t-shirt. She’s really not a dress person, tbh.

PSYCHOLOGY.

FEAR / S. Losing people, failing to save someone, being the only one left ASPIRATION / S.   Saving people, getting better at her job POSITIVE TRAITS.  Determined, selfless (in many ways),  NEGATIVE TRAITS.  Reckless, easily angered  MBTI.  ENTP ZODIAC. Leo TEMPEREMENT.  Choleric SOUL TYPE / S. Performer and Hunter ANIMALS. Eagle VICE HABIT / S.   FAITH. Catholic Christian/None/Judaism GHOSTS? Probably not AFTERLIFE? No REINCARNATION? No (even though I love those kinds of AUs lmao) ALIENS? No POLITICAL ALIGNMENT.  Not sure ECONOMIC PREFERENCE. Not sure SOCIOPOLITICAL POSITION. Liberal, probably. Not sure tbh. Claire really isn’t a politics person at all. EDUCATION LEVEL.  Low - only went to school up to age like 13

FAMILY.

FATHER.  Harold Hatcher MOTHER. Devorah Hatcher (née Lecuyer) SIBLINGS.   Harold “Harry Hatcher (b.1917) and James Hatcher (b.1919) EXTENDED  FAMILY.   Avigail Lecuyer (aunt), George “Georgie” Lecuyer (cousin, RAF bomber pilot, killed in 1944), Natanel Lecuyer (grandfather) and Esther Lecuyer (née Astruc, grandmother) NAME MEANING / S.   Claire: clear, famous Esther: star (in Persian) Hatcher: topographic name for someone who lived by a gate, from Middle English hacche (Old English hæcc) + the agent suffix -er. This normally denoted a gate marking the entrance to a forest or other enclosed piece of land, sometimes a floodgate or sluice-gate.

(I’m yelling I love that tbh)

HISTORICAL CONNECTION Claire is a traditionally French name, and Claire’s mum very much wanted to name one of her children something’s French. Esther is her grandmother’s name, and also a very important person in Judaism, and Devorah wanted to keep that connection, despite having converted from Judaism. 

FAVOURITES.

BOOK. Peter Pan MOVIE.  The Wizard Of Oz 5 SONGS.   (Going to do ones that kinda remind me of her again) Streetfight - Smallpools, 30 Seconds - Vinyl Theatre, Settle Down - The 1975, When That Man Is Dead And Gone - Glenn Miller, Run - Hozier DEITY.   None/the Jewish God HOLIDAY.  Rosh Hashanah  MONTH.   May SEASON.   Spring PLACE.   Downtown Portland WEATHER. Warm with a gentle breeze SOUND.  Rain on roof SCENT / S. Anything other than death and blood, tbh TASTE / S.  Coffee FEEL / S.  Soft hands on skin, water running over hands ANIMAL / S.   Cats, dogs NUMBER.  None COLOR.  The colours of the sunrise/sunset

EXTRA.

TALENTS.  Singing (a little bit), being salty/sarcastic, getting drunk BAD  AT.   Respect, listening, cooking, drawing, writing (nicely) TURN  ONS.  A lot of things TURN  OFFS.  Not a lot of things tbh HOBBIES.  Drinking, smoking, making sure her men are safe TROPES.  The Drunk Medic™ AESTHETICS.   Blood on snow, bruised knuckles, cocky smiles, dirty uniforms

FC INFO.

MAIN  FC / S. Eliza Taylor ALT  FC / S. - OLDER  FC / S.  - YOUNGER  FC / S. Sophie Nélisse VOICE  CLAIM / S.  Eliza Taylor’s American accent, but with some British in there

MUN QUESTIONS.

Q1.   if you could write your character your way in their own movie, what would it be called, what style would it be filmed in, and what would it be about? A1.  I would love to either see her in her canon setting, following what it would have potentially been like for a female medic if they would have been allowed in the US Army. Or like something where she’s the leader of like a biker gang or something, all in leather and looking fierce af.

Q2.   what would their soundtrack / score sound like? A2.  Similar to the Pacific Rim soundtrack probably, or TRON Legacy-vibes

Q3.   why did you start writing this character? A3. I had just rewatched Band Of Brothers and was home alone just hanging out for a week and I came up with a very basic character idea what I started working on and then made a blog for. Claire has obviously changed A LOT since then, but her basic version was a Band Of Brothers that pretty much right away turned fandomless, because I preferred that idea.

Q4.   what first attracted you to this character? A4. So many things... I love the idea of women in WWII and doing more than what they did, and I just wanted to explore writing a character that was one out of very few women doing something that has always been very male-oriented.

Q5.   describe the biggest thing you dislike about your muse. A5.  Oh man... She’s just so crazy reckless and just won’t care about her own life or safety, if it means she can save someone else.

Q6.   what do you have in common with your muse? A6.  Not that much, I don’t think. I mean, when I started out we had nothing except our gender in common, but writing her has kinda made me grow and I’m a lot more outspoken and stuff than I used to be? I also won’t stop talking about fighting people so... there’s that too.

Q7.   how does your muse feel about you? A7.  She probably hates me, let’s be real here.

Q8.   what characters does your muse have interesting interactions with? A8. I’ve loved all her interactions with Mick in the past, they’re so amazing. But right now, @hxllbilly and Claire are being two of my faves, and I’m really loving what I’ve either done or planned with @imhohenschloss . But tbh, I love most of the things I’ve done so far and I feel like most things are interesting, even though I do have my favourite threads. 

Q9.   what gives you the inspiration to write your muse? A9.  So many things, but war movies and shows always sparks some muse. Same does music and just a lot of things. She’s one of my strongest muses, tbh.

Q10. how long did this take you to complete? A10. 24+ hours, haha. Finishing this during Eurovision voting lmao.

[dorcasrempel]

Bringing RNA into genomics

The human genome contains about 20,000 protein-coding genes, but the coding parts of our genes account for only about 2 percent of the entire genome. For the past two decades, scientists have been trying to find out what the other 98 percent is doing.

A research consortium known as ENCODE (Encyclopedia of DNA Elements) has made significant progress toward that goal, identifying many genome locations that bind to regulatory proteins, helping to control which genes get turned on or off. In a new study that is also part of ENCODE, researchers have now identified many additional sites that code for RNA molecules that are likely to influence gene expression.

These RNA sequences do not get translated into proteins, but act in a variety of ways to control how much protein is made from protein-coding genes. The research team, which includes scientists from MIT and several other institutions, made use of RNA-binding proteins to help them locate and assign possible functions to tens of thousands of sequences of the genome.

“This is the first large-scale functional genomic analysis of RNA-binding proteins with multiple different techniques,” says Christopher Burge, an MIT professor of biology. “With the technologies for studying RNA-binding proteins now approaching the level of those that have been available for studying DNA-binding proteins, we hope to bring RNA function more fully into the genomic world.”

Burge is one of the senior authors of the study, along with Xiang-Dong Fu and Gene Yeo of the University of California at San Diego, Eric Lecuyer of the University of Montreal, and Brenton Graveley of UConn Health.

The lead authors of the study, which appears today in Nature, are Peter Freese, a recent MIT PhD recipient in Computational and Systems Biology; Eric Van Nostrand, Gabriel Pratt, and Rui Xiao of UCSD; Xiaofeng Wang of the University of Montreal; and Xintao Wei of UConn Health.

RNA regulation

Much of the ENCODE project has thus far relied on detecting regulatory sequences of DNA using a technique called ChIP-seq. This technique allows researchers to identify DNA sites that are bound to DNA-binding proteins such as transcription factors, helping to determine the functions of those DNA sequences.

However, Burge points out, this technique won’t detect genomic elements that must be copied into RNA before getting involved in gene regulation. Instead, the RNA team relied on a technique known as eCLIP, which uses ultraviolet light to cross-link RNA molecules with RNA-binding proteins (RBPs) inside cells. Researchers then isolate specific RBPs using antibodies and sequence the RNAs they were bound to.

RBPs have many different functions — some are splicing factors, which help to cut out sections of protein-coding messenger RNA, while others terminate transcription, enhance protein translation, break down RNA after translation, or guide RNA to a specific location in the cell. Determining the RNA sequences that are bound to RBPs can help to reveal information about the function of those RNA molecules.

“RBP binding sites are candidate functional elements in the transcriptome,” Burge says. “However, not all sites of binding have a function, so then you need to complement that with other types of assays to assess function.”

The researchers performed eCLIP on about 150 RBPs and integrated those results with data from another set of experiments in which they knocked down the expression of about 260 RBPs, one at a time, in human cells. They then measured the effects of this knockdown on the RNA molecules that interact with the protein.

Using a technique developed by Burge’s lab, the researchers were also able to narrow down more precisely where the RBPs bind to RNA. This technique, known as RNA Bind-N-Seq, reveals very short sequences, sometimes containing structural motifs such as bulges or hairpins, that RBPs bind to.

Overall, the researchers were able to study about 350 of the 1,500 known human RBPs, using one or more of these techniques per protein. RNA splicing factors often have different activity depending on where they bind in a transcript, for example activating splicing when they bind at one end of an intron and repressing it when they bind the other end. Combining the data from these techniques allowed the researchers to produce an “atlas” of maps describing how each RBP’s activity depends on its binding location.

“Why they activate in one location and repress when they bind to another location is a longstanding puzzle,” Burge says. “But having this set of maps may help researchers to figure out what protein features are associated with each pattern of activity.”

Additionally, Lecuyer’s group at the University of Montreal used green fluorescent protein to tag more than 300 RBPs and pinpoint their locations within cells, such as the nucleus, the cytoplasm, or the mitochondria. This location information can also help scientists to learn more about the functions of each RBP and the RNA it binds to.

“The strength of this manuscript is in the generation of a comprehensive and multilayered dataset that can be used by the biomedical community to develop therapies targeted to specific sites on the genome using genome-editing strategies, or on the transcriptome using antisense oligonucleotides or agents that mediate RNA interference,” says Gil Ast, a professor of human molecular genetics and biochemistry at Tel Aviv University, who was not involved in the research.

Linking RNA and disease

Many research labs around the world are now using these data in an effort to uncover links between some of the RNA sequences identified and human diseases. For many diseases, researchers have identified genetic variants called single nucleotide polymorphisms (SNPs) that are more common in people with a particular disease.

“If those occur in a protein-coding region, you can predict the effects on protein structure and function, which is done all the time. But if they occur in a noncoding region, it’s harder to figure out what they may be doing,” Burge says. “If they hit a noncoding region that we identified as binding to an RBP, and disrupt the RBP’s motif, then we could predict that the SNP may alter the splicing or stability of the gene.”

Burge and his colleagues now plan to use their RNA-based techniques to generate data on additional RNA-binding proteins.

“This work provides a resource that the human genetics community can use to help identify genetic variants that function at the RNA level,” he says.

The research was funded by the National Human Genome Research Institute ENCODE Project, as well as a grant from the Fonds de Recherche de Québec-Santé.

Bringing RNA into genomics syndicated from https://osmowaterfilters.blogspot.com/

[amalelrhazi]

Le masque de R-PUR filtre les particules fines

Amal El Rhazi, Le masque de R-PUR filtre les particules fines. Matthieu Lecuyer et Flavien Hello se sont connus à Séoul (Corée du Sud) durant leurs études. Mais c’est plus tard qu’ils ont décidé de se lancer dans la conception d’un masque antipollution, puis de créer la société parisienne R-PUR. "De retour à Paris, je suis tombé malade. Des […] Lire l'article

[heather-parady]

073- Women Succeeding in a Male Dominated Industry With Christina Lecuyer

Have you ever been afraid of showing up because of what other people may think of you? Have you ever held back from reaching your greatest potential because you’re afraid of others judging your worth? Odds are, we all have sold ourselves short at some point because we were afraid of what others might say. Being afraid of other people’s judgment isn’t limited to just men or just women. We all tend to put a halt on our greatness in order to fit into someone else’s mold. But, we weren’t meant to be what someone else thinks we should be.

Our guest this week, professional athlete and golf competitor, Christina Lecuyer believes that we all have something to share. We shouldn’t be afraid of what other people think of us because whatever we look like or whatever we do in life, these things don’t define our worth. What defines our worth and our success is the willingness of showing. If we aren’t showing up as our true selves and working towards our true purposes in life, we are robbing ourselves and the people we are called to reach. Christiana tells us to show up and own our greatness. The human movement relies on us showing up as who we are, one step at a time.

  Topics Discussed:

How women can feel confident in business while working in a male-dominated society.

How showing up every day allows you to step into your true calling

How to be more confident in life and business.

How to own your greatness.

How to overcome doubt.

  Connect with Christina:

Website

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[lepostde9h20]

De l’autre côté de la prise

Oui, ces lignes ont été rédigées en toute modestie par “le blogueur le plus branché de la ville”, comme vous l’apprenait hier (moi j’étais déjà au courant) l’excellent Thomas Lecuyer dans Lausanne-Cités.

Même si mon ego – encore plus crémé que le dos d’un touriste allemand dans un all inclusive d’Antalya – a adoré, je me dois de vous montrer l’envers du décor de la vie de celui qui “dégomme l’univers fermé des bobos lausannois à la manière d’un Frédéric Beigbeder ou d’un Nicolas Bedos, en plus sobre et moins chargé” (oui, je bande). Et oui, c’est un peu opportuniste de me remettre à écrire maintenant, mais il faut bien réagir, avant que l’article en question ne finisse dans une cheminée ou un container à papier.

Donc ce n’est pas tout à fait comme cela que je définirai mon quotidien. Par exemple, l’écriture de cet article a été entrecoupée par la réception de 53 mails professionnels. Alors que tout le monde sait que les gens branchés reçoivent des trucs bien plus stylés comme de la drogue, des invitations à des soirées mondaines et, par la suite, des MST. 

Et puis, les gens branchés n’ont pas ceci. Oui mesdames et messieurs, parlons un peu de la plus grande invention de l’Histoire depuis les clips pour sachets :

La litière automatique ! Vous devez être entrain de vous foutre moi, estimant que cet objet (200.- tout de même) est inutile. Vous trouvez ça gadget et pourtant vous savez que David Payot et Pierre-Antoine Hildebrand sont municipaux à Lausanne. S’il vous plaît un peu de cohérence… D’autant plus qu’ils coûtent bien plus que 200.-

Bon, nous sommes d’accord, cette vidéo n’a pas l’ombre d’un intérêt (à part la mienne que l’on devine dans le sable), et je vous dois 55 secondes de votre vie. Mais si l’humanité a pu créer ça ET un truc en plastique qui ne ferme rien du tout, je me dis qu’il y a de bons espoirs que nous réglions une bonne foi pour toutes la question de la pauvreté sur terre.

Non, vraiment pas grand chose de branché ces jours. Comme tout le monde, je me retrouve, de manière inexpliquée, à être fasciné par les interviews minimalistes des nouveaux médias sur les réseaux sociaux. L’autre jour, c’était une vidéo sous forme de “ni oui, ni non”. Vous qui êtes fâché pour la vidéo du caca de Bojack qui va tout seul dans le bac de ma caisse, dites-vous que j’ai passé 2 minutes devant cette chose hybride. A quand le “action ou vérité” de Pierre Maudet pour la Tribune de Genève* ?

Encore pire, encore un autre jour que le premier autre jour. J’étais occupé à travailler dans un endroit plutôt sécurisé contre les ringardises (le Pointu, 7 sur 10 sur l’échelle du Spritz et de la hype). Sans que je n’aie rien demandé, mes voisins de table se sont mis à évoquer leur consommation de thé, dans un dialogue qui n’aurait rien à envier au heures de gloire du cinéma suisse :

– Ah, mais qu’est-ce que je bois comme thé au collège moi ! (Avale une gorgée de son chaï “TripleLatte-DoubleFace-SaltoArrière”)

– Fais attention, tu risques d’uriner tellement que cela va diluer les bons éléments dans ton sang. Tu devrais boire aussi du café.

Outre la faible probabilité que quelqu’un d’autre qu’une grand-mère ne donne une fois ce conseil, il faut relever qu’il ne peut s’agir que d’enseignants, puisqu’il était 14h30 un mardi.

Non, actuellement mes préoccupations sont les suivantes. Préparez-vous, ça décoiffe :

Me stresser professionnellement pour pouvoir aller me détendre en vacances dès le 9 décembre.

Récupérer mon scooter que j’ai dû abandonner loin de chez moi (depuis que mon garagiste m’a dit “sortez-le pas quand il pleut”, je me méfie. Encore plus sous la neige).

Trouver l’énergie d’aller acheter un plan de travail mural et une petite table pour ma cuisine (dans ce genre-là, si vous avez des plans).

Décidément, personne n’est à l’abri de la médiocrité. Peut-être parce que nous sommes tous un peu nuls, au fond. Derrière les intitulés de nos jobs, sous nos fringues et sans la possibilité de consommer, nous sommes tous des glandeurs aussi nus que névrosés. Et je n’ai jamais vu quelqu’un que de nu déprimé, mais ça doit être ridicule. Même si l’on ravit des milliers de gens sur une scène, ou 5 sur un blog, derrière les rideaux et les écrans, la vie est tristement normale. Bon, pour certains, c’est plus triste (quand tu as une litière automatique dans ton appartement ou que tu es Yannick Buttet dans un jardin) que pour d’autres.

Mais rassurez-vous le fun arrive. Demain, la bourse aux armes commence à Beaulieu et je crois que je vais pouvoir vous en parler la semaine prochaine sur ce blog.

* Merci à Marianne Grosjean de me commissionner sur sa future augmentation pour cette idée géniale.