prplotion

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PRP每月科技新知 (2023/06/02)- PRP在皮膚病學中有許多應用- 2. 皮膚年輕化

富血小板血漿 (PRP) 是一種含有高濃度血小板和生長因子的自體血清。 PRP 持續發展成為一種重要的治療方式，在皮膚病學中有許多應用，特別是在頭髮修復、皮膚再生、痤瘡疤痕、真皮增大和萎縮紋等領域。 此外，將 PRP 與雷射治療、微針、真皮填充劑和自體脂肪移植共同使用會產生協同效應，從而改善美學效果。 未來的研究應該針對特定適應症對 PRP 治療方式進行標準化。 隨著治療應用的不斷擴大，PRP 在皮膚病學領域具有相當大的前景。

(圖示為使用多種醫美產品，包括皮下填充、射頻、抽脂、肉毒及 PRP的前後照：女性全臉年輕化，注意皮膚質地、顏色和色調的顯著改善。組合方法：填充劑改善臉頰、下巴和嘴唇的外側；內部射頻 (InMode FaceTite™) 和下臉和頸部吸脂術；用於化學提眉（眉間“11”紋）和眼部皺紋（魚尾紋）的 Botox® Cosmetic；微針射頻 (InMode Fractora™) 與 PRP 在 12 個月內進行了一系列的三種治療。)

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Platelet-rich plasma (PRP) is an autologous serum containing high concentrations of platelets and growth factors. PRP continues to evolve as an important treatment modality with many applications in dermatology, particularly in the areas of hair restoration, skin rejuvenation, acne scars, dermal augmentation, and striae distensae. Furthermore, combining PRP with laser therapies, microneedling, dermal fillers, and autologous fat grafting produces synergistic effects, leading to improved aesthetic results. Future studies should standardize PRP treatment protocols for specific indications. PRP holds considerable promise in dermatology with therapeutic applications continuing to expand.

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PRP每月科技新知 (2023/05/03)- PRP在皮膚病學中有許多應用- 1. 頭髮修復

富血小板血漿 (PRP) 是一種含有高濃度血小板和生長因子的自體血清。 PRP 持續發展成為一種重要的治療方式，在皮膚病學中有許多應用，特別是在頭髮修復、皮膚再生、痤瘡疤痕、真皮增大和萎縮紋等領域。 此外，將 PRP 與雷射治療、微針、真皮填充劑和自體脂肪移植共同使用會產生協同效應，從而改善美學效果。 未來的研究應該針對特定適應症對 PRP 治療方式進行標準化。 隨著治療應用的不斷擴大，PRP 在皮膚病學領域具有相當大的前景。

(圖示為注射 PRP後，男性/女性 頭髮密度和顏色皆顯著增加)

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Platelet-rich plasma (PRP) is an autologous serum containing high concentrations of platelets and growth factors. PRP continues to evolve as an important treatment modality with many applications in dermatology, particularly in the areas of hair restoration, skin rejuvenation, acne scars, dermal augmentation, and striae distensae. Furthermore, combining PRP with laser therapies, microneedling, dermal fillers, and autologous fat grafting produces synergistic effects, leading to improved aesthetic results. Future studies should standardize PRP treatment protocols for specific indications. PRP holds considerable promise in dermatology with therapeutic applications continuing to expand.

文章出處: Jason Emer. Platelet-Rich Plasma (PRP): Current Applications in Dermatology. Skin Therapy Letter 2019;24(5).

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PRP每月科技新知 (2023/3/28)- PRP可減輕急性放射治療副作用

研究背景: 暴露於高劑量放射治療所引起的皮膚傷口是臨床治療的重大挑戰。雖然目前有許多新的皮膚治療方式正在研究中，但尚未證實任何皮膚療法可用於治療放射性皮膚炎的損傷或是可以促進受損組織的修復。PRP是由富含血小板的血漿製成的具有生物活性的生物材料 (這篇文章寫做Plasma-based biomaterials, PBMs)，可以製成固體和半固體形式，價格相對低廉，可作為現成的非冷藏產品。

研究方法: 在這項研究中，使用小鼠模型來研究PRP減少放射性皮膚損傷的急性和晚期副作用。使用單劑量35格雷(Gy)照射直徑為2公分的背部皮膚照野，然後每天一次使用PRP進行局部治療，在放射治療後共使用5週。每週成像顯示，使用PRP的傷口恢復更好: 在最大傷口面積後第 12、13 和 14 週時變得具有統計學意義 (p < 0.05)。

結論: PRP可有效促進傷口修復，進而減輕急性放射治療副作用。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Cutaneous wounds caused by an exposure to high doses of ionizing radiation remain a therapeutic challenge. While new experimental strategies for treatment are being developed, there are currently no off-the-shelf therapies for the treatment of cutaneous radiation injury that have been proven to promote repair of the damaged tissues. Plasma-based biomaterials are biologically active biomaterials made from platelet enriched plasma, which can be made into both solid and semi-solid forms, are inexpensive, and are available as off-the-shelf, non-refrigerated products. In this study, the use of plasma-based biomaterials for the mitigation of acute and late toxicity for cutaneous radiation injury was investigated using a mouse model. A 2-cm diameter circle of the dorsal skin was irradiated with a single dose of 35 Gy followed by topical treatment with plasma-based biomaterial or vehicle once daily for 5 weeks post-irradiation. Weekly imaging demonstrated more complete wound resolution in the plasma-based biomaterial vs. vehicle group which became statistically significant (p < 0.05) at weeks 12, 13, and 14 post-maximum wound area. Despite more complete wound healing, at 9 and 17 weeks post-irradiation, there was no statistically significant difference in collagen deposition or skin thickness between the plasma-based biomaterial and vehicle groups based on Masson trichrome staining nor was there a statistically significant difference in inflammatory or fibrosis-related gene expression between the groups. Although significant improvement was not observed for late toxicity, plasma-based biomaterials were effective at promoting wound closure, thus helping to mitigate acute toxicity.

文章出處:

Miller ED, Song F, Smith JD, Ayan AS, Mo X, Weldon M, Lu L, Campbell PG, Bhatt AD, Chakravarti A et al: Plasma-based biomaterials for the treatment of cutaneous radiation injury. Wound Repair Regen 2019, 27(2):139-149.

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PRP每月科技新知 (2023/03/01)- PRP可用於預防掉髮

研究背景: 放射治療(放療)所造成的放射性皮膚炎會嚴重影響患者的生活品質，在某些情況下還會影響整形重建的癒合時間。富含血小板血漿（PRP）是一種新興療法。 PRP目前的用途包括：減緩局部發炎、促進傷口癒合、治療放療所引起的皮膚潰瘍，其用途延伸到美容整形外科領域，改善皮膚品質。

研究目的: 使用 PRP 作為放療後皮膚毛髮損傷的預防。

研究方式: 使用12 隻 Wistar 大鼠進行雙盲試驗。由其中的2隻大鼠提供捐血獲得PRP，另外10隻用於臨床和組織學分析。

結果: 在放療前沒有使用 PRP 的區域：8 隻大鼠出現脫髮和 7隻大鼠發生局部色素減退，其中60% 出現完全脫髮，20% 出現部分脫髮，20% 未出現脫髮。在放療前有使用 PRP 的區域：4 隻出現脫髮和 3 隻色素減退，其中無完全脫髮病例，40%出現部分脫髮，60%未出現脫髮。從組織學分析中收集的數據顯示，有使用PRP 的那些區域中，100% 出現輕至中度萎縮；另一方面，沒有使用 PRP 的區域，80% 出現中至重度萎縮，20% 出現輕至中度萎縮。

結論: PRP 可用於預防脫髮。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Background and Objectives. Radiotherapy is usually associated with skin complications that affect the quality of life of patients and in some cases can also affect the timing of the reconstruction. The platelet rich plasma (PRP) is an emerging therapy; is autologous, safe and economic. Current use of PRP includes: efficacy in decreasing local inflammation, enhance wound healing, treatment of skin ulcers caused by radiotherapy and its use extends to the field of aesthetic plastic surgery improving the quality of the skin. The authors made an experimental trial to analyze the use of PRP as a prophylaxis method for skin damage after radiotherapy. We analyze the benefits of PRP as a prophylaxis method for skin damage after radiotherapy using an experimental model.

Methods. We conduct a double blind trial with rats. Twelve Wistar rats were used, 2 for obtaining the PRP and 10 for clinical and histological analysis.

Results. Data collected from the clinical analysis showed that those areas without PRP: 8 rats presented alopecia and 7 hypopigmentation. Total alopecia was present in 60%, partial alopecia in 20%, and 20% did not present alopecia. In those areas with PRP before radiotherapy: 4 presented alopecia and 3 hypopigmentation. There were no cases of total alopecia, 60% did not present alopecia and 40% presented partial alopecia. Data collected from the histological analysis showed 100% of mild-moderate atrophy in those areas with PRP. On the other hand, 80% of moderate-severe atrophy and 20% of mild-moderate atrophy was present on those areas without PRP.

Conclusions. The authors showed the benefit of PRP as a prophylaxis method for alopecia.

文章出處: D'Oliveira-Pazos L, Fossati-Cola J-M, Wolff-de-Freitas D-A, Luongo-Gardi Á, Otero M, Carrasco B: Use of platelet rich plasma as prophylactic method for skin damageafter radiotherapy: experimental study. Cirugia Plastica Ibero-Latinoamericana 2016, 42:109-114.

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PRP每月科技新知 (2023/02/02)- 再次證實PRP的抗纖維化作用

研究背景：研究PRP的抗纖維化效果。檢測 PRP 對乙型轉化生長因子 (TGF)-β1 所誘導的纖維母細胞(fibroblasts)分化為肌纖維母細胞(Myofibroblast)的影響(這是纖維化的主要因素)；並研究使用 PRP對於的血管內皮生長因子 (VEGF)-A 的作用；也評估單獨使用 PRP 對於纖維母細胞分化的影響。

研究方法: 使用以下方法來評估肌纖維母細胞的細胞表型: 共聚焦螢光顯微鏡、使用西方墨點法來鑑定α-平滑肌肌動蛋白 (alpha-smooth muscle actin, sma) 和 第1型膠原蛋白(type-1 collagen)的表現量、富含黏着斑蛋白的局部沾黏(vinculin-rich focal adhesion clustering)、應力纖維組件(stress fiber assembly)。使用 RT-PCR 分析 Notch-1、連接蛋白 43 (connexin 43) 和 VEGF-A 的表達。

結果: PRP 會通過 VEGF-A/VEGF 受體 (VEGFR)-1 所媒介的 TGF-beta1/Smad3 訊息傳遞路徑抑制負調節纖維母細胞-肌纖維母細胞的轉化。共同使用TGF-β1/PRP 的纖維母細胞顯示出肌纖維母細胞表型的強烈衰減，同時 Smad3 表達量降低。 抑制VEGFR-1 (如使用KRN633，這是一種血管內皮生長的抑制劑)會減少 PRP 的效果。此外，PRP 會消除 TGF-β1 所誘導的 VEGF-A 和 VEGFR-1 表達量減少。

結論: 使用 VEGF-A 進行細胞研究可證實 VEGF-A 信號傳導在對抗肌纖維母細胞生成中的作用。 PRP 作為單一治療不會誘導纖維母細胞肌分化。這項研究對於 PRP 的抗纖維化作用的細胞和分子機制提供了新的見解。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

The antifibrotic potential of platelet-rich plasma (PRP) is controversial. This study examined the effects of PRP on in vitro transforming growth factor (TGF)-beta1-induced differentiation of fibroblasts into myofibroblasts, the main drivers of fibrosis, and the involvement of vascular endothelial growth factor (VEGF)-A in mediating PRP-induced responses. The impact of PRP alone on fibroblast differentiation was also assessed. Myofibroblastic phenotype was evaluated by confocal fluorescence microscopy and western blotting analyses of alpha-smooth muscle actin (sma) and type-1 collagen expression, vinculin-rich focal adhesion clustering, and stress fiber assembly. Notch-1, connexin 43, and VEGF-A expression were also analyzed by RT-PCR. PRP negatively regulated fibroblast-myofibroblast transition via VEGF-A/VEGF receptor (VEGFR)-1-mediated inhibition of TGF-beta1/Smad3 signaling. Indeed TGF-beta1/PRP co-treated fibroblasts showed a robust attenuation of the myofibroblastic phenotype concomitant with a decrease of Smad3 expression levels. The VEGFR-1 inhibition by KRN633 or blocking antibodies, or VEGF-A neutralization in these cells prevented the PRP-promoted effects. Moreover PRP abrogated the TGF-beta1-induced reduction of VEGF-A and VEGFR-1 cell expression. The role of VEGF-A signaling in counteracting myofibroblast generation was confirmed by cell treatment with soluble VEGF-A. PRP as single treatment did not induce fibroblast myodifferentiation. This study provides new insights into cellular and molecular mechanisms underpinning PRP antifibrotic action.

文章出處:

Chellini F, Tani A, Vallone L, Nosi D, Pavan P, Bambi F, Zecchi Orlandini S, Sassoli C: Platelet-Rich Plasma Prevents In Vitro Transforming Growth Factor-beta1-Induced Fibroblast to Myofibroblast Transition: Involvement of Vascular Endothelial Growth Factor (VEGF)-A/VEGF Receptor-1-Mediated Signaling (dagger). Cells 2018, 7(9).

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PRP每月科技新知 (2023/1/17)- 蘆薈"無法"預防放射性皮膚炎的發生率和嚴重度

研究目的： 放射治療所引起的皮膚炎(以下簡稱放射性皮膚炎)是一種常見且有時很嚴重的副作用。本文章是隨機性研究，以了解使用蘆薈凝膠是否可以降低放射性皮膚炎的發生率及嚴重度。

研究方法： 100 名新診斷的乳癌患者在放射治療期間隨機接受蘆薈凝膠或不接受蘆薈凝膠，觀察並記錄其副作用。放射治療使用 6 MV 直線加速器的光子體外照射，總劑量為 50 格雷，每天 2 格雷，每週 5 次。

研究結果： 在第 2 週後兩組患者首次發現放射性皮膚炎。在蘆薈組和對照組中，2/8 名患者和 5/12 名患者在第 2 週後出現 第1級放射性皮膚炎（P 值 = 0.240）和第3級放射性皮膚炎（P 值 = 0.317）。 第 4 週後，數字為 18 例，23 例為 1 級放射性皮膚炎，只有 1 例為 3 級放射性皮膚炎（P 值 = 0.328）。 第 5 週後，蘆薈組有 31、12 和 2 名患者，對照組有 36、6 和 5 名患者分別出現 1、2 和 3 級放射性皮膚炎（P 值 = 0.488）。

結論：在本研究中，蘆薈對放射性皮膚炎的發生率和嚴重程度沒有影響。 # 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Purpose: Radiotherapy induced dermatitis is a common and sometimes serious side effect. We conducted a randomized study to understand whether the adjunctive use of aloe vera gel might reduce the prevalence and/or severity of radiotherapy induced dermatitis.

Methods: One hundred patients with newly diagnosed breast cancer were randomized to receive aloe vera gel or nothing during adjuvant radiotherapy consisting of conventional external beam radiation using 6 MV mega voltage linear accelerator photons. The radiation portals were composed of breast fields in all patients and supraclavicular and posterior axillary fields in node positive cases. The total dose was 50 Gy with a daily fraction of 2 Gy, five fractions per week.

Results: Dermatitis was first found among patients of both groups after week 2. In the aloe vera and control groups, 2/8 patients and 5/12 patients had dermatitis grade 1 after weeks 2 (P value = 0.240) and 3 (P value = 0.317). After the 4th week, the numbers were 18 and, 23 for dermatitis grade 1 and only 1 for grade 3 dermatitis (P value = 0.328). After the 5th week, 31, 12 and 2 patients in the aloe vera group and 36, 6 and 5 of the controls had grades 1, 2 and 3 dermatitis, respectively (P value = 0.488).

Conclusion: Aloe vera exerted no positive effect on prevalence or severity of radiation dermatitis in this study.

文章出處:

N. Ahmadloo, B. Kadkhodaei, S. Omidvari, A. Mosalaei, M. Ansari, H. Nasrollahi, et al. Asian Pac J Cancer Prev 2017 Vol. 18 Issue 4 Pages 1139-1143

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PRP每月科技新知 (2022/11/29)- PRP乳液可以有效預防放射性皮膚炎的不適感- 初步成果報告

狂賀!!! 2022年11月19日刊登於 「台灣癌症安寧緩和醫學會年會暨學術研討會」

三軍總醫院放射腫瘤部 使用PRP乳液用於放射性皮膚炎的臨床研究觀察一年，發現PRP乳液可避免放射治療所引起的皮膚嚴重副作用、可減少放射治療期間的皮膚不適感、減少放射治療引起的疼痛感，且可避免中斷放射治療。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

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PRP每月科技新知 (2022/10/31)- PRP可有效促進放射線皮膚炎癒合

研究背景：體外放射治療是癌症治療中非常重要的一環，但是，輻射線會導致嚴重的副作用，例如放射性皮膚炎，導致皮膚損傷與潰爛。此篇文章的研究目的主要是評估下列三種方式用於治療輻射引起的皮膚損傷的效果: 評估單獨使用微脂肪 (microfat)、微脂肪+ PRP、微脂肪+血管基質組份(SVF)。

研究方法：隨機使用5個實驗組，每組9隻小鼠：1個健康對照組和4個放射治療組（60 格雷）。在放射治療後3個月，使用下列方式來治療皮膚傷口；微脂肪、微脂肪+PRP（50%-50%）、微脂肪+SVF（90%-10%）、乳酸鈉溶液進行皮下注射。 在用藥後1、2和3個月評估���口癒合情況，並在第3個月安樂死小鼠進行組織學傷口分析。

結果：所有接受放射治療的小鼠均出現傷口。在給藥後，對照組的傷口面積增加了 91.1%、單獨使用微脂肪的傷口面積減少了15.9%、微脂肪+SVF的傷口面積減少了 27.3%、微脂肪+ PRP的傷口面積減少了76.4% (P=0.032)。

結論：微脂肪+ PRP是促進放射線皮膚炎癒合最有效的產品。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

BACKGROUND: External radiotherapy has become indispensable in oncological therapies. Unfortunately, radiation is responsible for serious side effects, such as radiodermatitis. The skin is weakened and ulcerated. Our study aimed to evaluate the subcutaneous transfer of microfat (MF) alone and two mixes: MF+Platelet-rich plasma (PRP) and MF+stromal vascular fraction (SVF) to treat radiation-induced skin lesions.

METHOD: We defined randomly five experimental groups of nine mice: 1 healthy control group and 4 irradiated (60 Gy) and treated groups. The skin lesions were treated 3 months after irradiation by MF, MF+PRP (50%-50%), MF+SVF (90%-10%) or Ringer-lactate subcutaneous injections. Wound healing was evaluated at 1, 2 and 3 months post-injection and histological wound analysis at 3 months, after euthanasia.

RESULTS: All the irradiated mice presented with wounds. After sham-injection, the wound area increased by 91.1+/-71.1% versus a decrease of 15.9+/-23.1% after MF alone (NS), 27.3+/-23.8% after MF+SVF (NS) and 76.4+/-7.7% after MF+PRP (P=0.032). A significantly reduction of skin thickness in wound periphery was measured for the three treated groups compared to sham-injection (P<0.05) but not in the healed wounds (NS). The most important subcutaneous neo-vessel density was shown after MF+SVF injection.

CONCLUSION: The MF+PRP mix was the most efficient product to increase healing. The MF+SVF mix showed the highest rate of neo-angiogenesis but was disappointing in terms of healing.

文章出處:

Bertrand B, Eraud J, Velier M, Cauvin C, Macagno N, Boucekine M, Philandrianos C, Casanova D, Magalon J, Sabatier F: Supportive use of platelet-rich plasma and stromal vascular fraction for cell-assisted fat transfer of skin radiation-induced lesions in nude mice. Burns 2020.

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PRP每月科技新知 (2022/09/30)- PRP會加速放射線照射後的皮膚再生、恢復皮脂腺功能、增加膠原蛋白產生、減少皮膚水分流失

研究背景：各種形式的放射線都會造成皮膚損傷。然而，針對嚴重、急性放射性皮膚損傷的治療選擇很有限。目前已知PRP可改善皮膚傷口癒合，但PRP對於高劑量放射線照射的效果仍知之甚少。

研究方法：使用小鼠背部皮膚進行局部照射40格雷(Gy)，並使用伽馬射線5格雷來照射HaCaT細胞(這是一種來自於成年人類的永生化角質形成細胞的細胞株)用於研究 PRP的再生功能。利用評估傷口大小、組織學特徵、免疫染色、西方墨點法和經皮水分流失(TEWL)來評估PRP對於皮膚的益處。為了評估PRP對於輻射傷口的癒合狀況與角質形成細胞的分子影響，我們評估了AKT訊息傳遞路徑。

結果：儘管經輻射受損的HaCaT細胞癒合能力下降，但在照射後的HaCaT細胞和皮膚組織中，角質細胞14(Keratin 14, K14)的表現量升高。使用PRP後，可以經由增強AKT訊息傳遞恢復皮膚癒合能力。在我們的小鼠模型中，使用PRP可證實以下結果：（1）增加放射性皮膚炎的皮膚癒合能力；(2)激活AKT訊息傳遞，改善角質細胞K14的遷移和增殖；(3) 增加角質細胞K10與皮脂腺的內披蛋白表現能力；(4)減少經皮水分流失TEWL，加強皮膚屏障功能。

結論：PRP可藉由AKT訊息傳遞，而增強角質細胞K14的功能，加速放射線照射後的皮膚再生。對於臨床的放射性傷口癒合有重大助益。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

BACKGROUND: The skin is impacted by every form of external radiation therapy. However, effective therapeutic options for severe, acute radiation-induced skin reactions are limited. Although platelet-rich plasma (PRP) is known to improve cutaneous wound healing, its effects in the context of high-dose irradiation are still poorly understood.

METHODS: We investigated the regenerative functions of PRP by subjecting the dorsal skin of mice to local irradiation (40 Gy) and exposing HaCaT cells to gamma rays (5 Gy). The cutaneous benefits of PRP were gauged by wound size, histologic features, immunostains, western blot, and transepithelial water loss (TEWL). To assess the molecular effects of PRP on keratinocytes of healing radiation-induced wounds, we evaluated AKT signaling.

RESULTS: Heightened expression of keratin 14 (K14) was documented in irradiated HaCaT cells and skin tissue, although the healing capacity of injured HaCaT cells declined. By applying PRP, this capacity was restored via augmented AKT signaling. In our mouse model, PRP use achieved the following: (1) healing of desquamated skin, acutely injured by radiation; (2) activated AKT signaling, improving migration and proliferation of K14 cells; (3) greater expression of involucrin in keratin 10 cells and sebaceous glands; and (4) reduced TEWL, strengthening the cutaneous barrier function.

CONCLUSIONS: Our findings indicate that PRP enhances the functions of K14 cells via AKT signaling, accelerating the regeneration of irradiated skin. These wound-healing benefits may have merit in a clinical setting.

文章出處: Lee J, Jang H, Park S, Myung H, Kim K, Kim H, Jang WS, Lee SJ, Myung JK, Shim S: Platelet-rich plasma activates AKT signaling to promote wound healing in a mouse model of radiation-induced skin injury. J Transl Med 2019, 17(1):295.

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PRP每月科技新知 (2022/08/30)- PRP是面部皮膚年輕化（回春）的有效方式

研究背景：富含血小板血漿（PRP）是一種濃縮的血小板血漿，最近被證實PRP內含多種生長因子和細胞粘附分子可以加速老化皮膚的年輕化(回春)。

研究目的： 評估皮下注射PRP在面部年輕化中的有效性和安全性。

方法： 本研究是一項前瞻性、單中心、單劑量、開放���、非隨機對照臨床研究。 PRP 注射到右耳下區域的上部和整個面部，並在左耳下區域注射鹽水當做對照組。在 PRP 治療前、PRP 後 28 天和鹽水（對照組）治療後進行組織病理學檢查。

結果： 20 名年齡在 40 至 49 歲（平均年齡為 43.65+/-2.43 歲）的女性參加此研究。測量PRP治療前、對照組和 PRP 治療後的區域中膠原蛋白的平均光密度 (MOD): 分別為 539+/-93.2、787+/-134.15、1,019+/-178。PRP 治療後的 MOD 與PRP治療前的 MOD 相比，改善了 89.05%，也就是膠原纖維的平均 MOD 在 PRP 側明顯最高（p<0.001）。 PRP 與生理鹽水的改善比率（89.05% 至 46.01%）為 1.93:1。沒有檢測到嚴重的副作用。

結論：PRP 可藉由生長因子或皮膚滾針來增加真皮層膠原蛋白。 PRP是面部皮膚年輕化（回春）的有效方式。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

BACKGROUND: Platelet-rich plasma (PRP) is an autologous concentration of human platelets contained in a small volume of plasma and has recently been shown to accelerate rejuvenate aging skin by various growth factors and cell adhesion molecules. OBJECTIVE: This study was conducted to evaluate the efficacy and safety of intradermal injection of PRP in the human facial rejuvenation.

METHODS: This study was a prospective, single-center, single-dose, open-label, non-randomized controlled clinical study. PRP injected to the upper site of this right infra-auricular area and all face. Saline was injected to the left infra-auricular area. Histopathological examinations were performed before PRP treatment, 28 days after the PRP, and saline (control) treatments.

RESULTS: Twenty women ranging in age from 40 to 49 years (mean age, 43.65+/-2.43 years) were enrolled in the study. The mean optical densities (MODs) of collagen in the pre-treatment, control, and PRP-treated area were measured. They were 539+/-93.2, 787+/-134.15, 1,019+/-178, respectively. In the MOD of PRP, 89.05 percent improvement was found when MOD of PRP was compared with MOD of pre-treatment. The mean MOD of collagen fibers was clearly highest on the PRP side (p<0.001). The PRP-to-saline improvement ratio (89.05% to 46.01%) was 1.93:1. No serious side effects were detected.

CONCLUSION: PRP increases dermal collagen levels not only by growth factors, but also by skin needling (the mesotherapy technique 'point by point'). PRP application could be considered as an effective (even a single application) and safety procedure for facial skin rejuvenation.

文章出處: Abuaf OK, Yildiz H, Baloglu H, Bilgili ME, Simsek HA, Dogan B: Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study. Ann Dermatol 2016, 28(6):718-724.

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PRP每月科技新知 (2022/08/05)- PRP可減輕急性放射治療副作用

研究背景: 暴露於高劑量放射治療所引起的皮膚傷口是臨床治療的重大挑戰。雖然目前有許多新的皮膚治療方式正在研究中，但尚未證實任何皮膚療法可用於治療放射性皮膚炎的損傷或是可以促進受損組織的修復。PRP是富含血小板的血漿製成的具有生物活性的生物材料(這篇文章寫做Plasma-based biomaterials, PBMs)，可以製成固體和半固體形式，價格相對低廉，可作為現成的非冷藏產品。

研究方法: 在這項研究中，使用小鼠模型來研究PRP減少放射性皮膚損傷的急性和晚期副作用。使用單劑量35格雷(Gy)照射直徑為2公分的背部皮膚照野，然後每天使用PRP進行局部治療，在放射治療後共使用5週。每週成像顯示，使用PRP的傷口恢復更好: 在最大傷口面積後第 12、13 和 14 週時變得具有統計學意義 (p < 0.05)。

結論: PRP可有效促進傷口修復，進而減輕急性放射治療副作用。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Cutaneous wounds caused by an exposure to high doses of ionizing radiation remain a therapeutic challenge. While new experimental strategies for treatment are being developed, there are currently no off-the-shelf therapies for the treatment of cutaneous radiation injury that have been proven to promote repair of the damaged tissues. Plasma-based biomaterials are biologically active biomaterials made from platelet enriched plasma, which can be made into both solid and semi-solid forms, are inexpensive, and are available as off-the-shelf, nonrefrigerated products. In this study, the use of plasma-based biomaterials for the mitigation of acute and late toxicity for cutaneous radiation injury was investigated using a mouse model. A 2-cm diameter circle of the dorsal skin was irradiated with a single dose of 35 Gy followed by topical treatment with plasma-based biomaterial or vehicle once daily for 5 weeks postirradiation. Weekly imaging demonstrated more complete wound resolution in the plasma-based biomaterial vs. vehicle group which became statistically significant (p < 0.05) at weeks 12, 13, and 14 postmaximum wound area. Despite more complete wound healing, at 9 and 17 weeks postirradiation, there was no statistically significant difference in collagen deposition or skin thickness between the plasma-based biomaterial and vehicle groups based on Masson trichrome staining nor was there a statistically significant difference in inflammatory or fibrosis-related gene expression between the groups. Although significant improvement was not observed for late toxicity, plasma-based biomaterials were effective at promoting wound closure, thus helping to mitigate acute toxicity.

文章出處:

Miller ED, Song F, Smith JD, Ayan AS, Mo X, Weldon M, Lu L, Campbell PG, Bhatt AD, Chakravarti A et al: Plasma-based biomaterials for the treatment of cutaneous radiation injury. Wound Repair Regen 2019, 27(2):139-149.

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PRP每月科技新知 (2022/07/01)- PRP是乾癬患者的治療新選擇

研究背景: 乾癬是一種慢性免疫相關的疾病，盛行率大約 2-3%，對患者的身體和心理生活品質產生顯著的負面影響，也會產生心臟代謝功能障礙及憂鬱症等合併症。此研究評估富含血小板血漿 (PRP) 在治療慢性局限性頑固性斑塊狀乾癬患者的效果。

研究方法: 這是一項前瞻性創新研究。

研究結果: 共收集30例慢性局限性頑固性乾癬患者。與基線相比，治療 8 周和 16 週後，第1組的 PASI (乾癬面積和嚴重程度指數) 評分顯著降低（p = 0.001）。���第8周和第16週之間，PASI 評分也顯著下降（p = 0.0018）。與基線相比，第8周和第16週的第2組 PASI 評分均無顯著差異（p = 0.894）。

結論: PRP為慢性局限性頑固性乾癬患者提供了一種有希望的新治療選擇。對於一般用藥效果不佳的乾癬患者，可使用 PRP 來治療。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Psoriasis is a chronic immune-mediated disease affecting approximately 2–3% of general population. It is associated with significant negative impact on patient’s physical and psychological quality of life along with vulnerability to co-morbidities such as cardio-metabolic dysfunction, depression, and so on [1]. to evaluate the efficiency of platelet rich plasma (PRP) in the management of patients with chronic localized recalcitrant plaque psoriasis. This was a prospective pilot study conducted on 30 cases with chronic localized recalcitrant psoriasis attended Dermatology, Venereology and Andrology outpatient clinics, Benha University Hospital. This study was approved by Dermatology, Venereology and Andrology Department and Ethics Commitee in the Faculty of Medicine, Benha University. The study details were discussed with all patients and informed consents were obtained from them before the beginning of the study. PASI (Psoriasis Area and Severity Index) score had significantly decreased in group I after 8 and 16 weeks of treatment when compared to the baseline (p =0.001). There was also significant decrease in PASI score between week 8, 16 (p =0.0018). PASI score showed no significant difference in group II neither at week 8 nor 16 compared to baseline (p =0.894).

Conclusion: Platelet Rich Plasma (PRP) therapy represents a promising new therapeutic option for the patients with chronic localized recalcitrant psoriasis Plaques. The ability to use PRP could resolve several problems occurring with other treatment modalities of psoriasis. 文章出處:

Abd-El Rahman S.H., Salem RM and Ahmed AH. Evaluation of Platelet Rich Plasma in the Management of Psoriasis. Benha Journal of Applied Sciences (BJAS) 2018;3(1):21-25.

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PRP每月科技新知 (2022/06/01)- PRP可用於異位性皮膚炎和其他類型的濕疹

---這是一篇個案報告---

前言: 異位性皮膚炎是一種慢性皮膚發炎疾病，最常見於兒童時期，但也影響許多成年人。大多數異位性皮膚炎患者使用保濕劑和外用類固醇進行治療。然而，這些治療並不總是能夠良好控制疾病。富含血小板血漿 (PRP) 用於治療許多皮膚病。PRP是經由血小板釋放各種物質（例如生長因子）發揮作用，從而誘導傷口癒合的一系列反應，包括抗發炎和組織再生，從而促進皮膚健康。這篇文章報導了一例異位性皮膚炎患者，使用 PRP 成功控制皮膚症狀。

個案報告: 一名 44 歲的女性於 2016 年 5 月就診，有長期反覆面部濕疹病史。患者使用類固醇乳膏六個月後，效果不佳，並且也詢問眼眶下黑眼圈和皺紋的治療方法。在討論治療方式後，決定進行 PRP 注射。 使用 4-cc 富含血小板的纖維蛋白基質試劑盒 (Selphyl, Aesthetic Factors, Inc) 進行了兩次 PRP 針劑注射治療，每次間隔 6 週。 除了在眼睛下方施用外，PRP 也使用在鼻唇溝中以達到年輕化的目的。

圖 1：面部濕疹對 PRP 針劑注射治療的效果。

結論: 根據這篇文章和其他研究的結果，PRP 可用於治療異位性皮膚炎和其他類型的濕疹。這位病人追蹤觀察兩年，症狀緩解是前所未有的。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp?tag=LINE

原文摘要:

Atopic dermatitis (AD) is a chronic inflammatory skin condition that most often presents in childhood but also affects many adults. Most patients with AD are treated with moisturizers and topical corticosteroids. However, these treatments do not always allow for an adequate control of the disease. Platelet-rich plasma (PRP) is used in treating many dermatologic conditions. It works by releasing bioagents, such as growth factors, from platelets, which induce a wound-healing cascade involving inflammation and tissue regeneration that promotes skin health. Here, we report a case of AD in which the patient successfully achieved an extended control of skin symptoms with PRP therapy.

    A 44-year-old woman presented in May 2016 with a long-standing history of recurrent facial eczema. Six months later, the patient returned for a refill of the hydrocortisone cream and inquired about treatments for infraorbital dark circles and wrinkles. After discussing the treatment options, we decided to proceed with PRP injections, and informed consent was obtained. Two PRP therapy sessions were performed, spaced 6 weeks apart, using a 4-cc platelet-rich fibrin matrix kit (Selphyl, Aesthetic Factors, Inc). In addition to administration beneath the eyes, PRP was also administered into nasolabial folds for rejuvenation purposes.

Fig 1: Response of facial eczema to PRP treatment.

Based on our results and those of other studies, there is an indication for the potential use of PRP in the management of AD and other types of eczema. Two years of symptom relief is unprecedented. A randomized clinical trial with a greater number of participants is warranted to rigorously evaluate the seemingly positive effects of PRP in treating skin eczema, including AD. Further research may also shed more light on the pathophysiology of AD as well as the biochemical pathways through which PRP can exert its therapeutic effects.

文章出處:

Saba Vafaei-Nodeh, BSc, and Shadan Kabiri-Abyaneh, MD, CCFP. Long-term control of atopic dermatitis with platelet-rich plasma. JAAD Case Rep. 2020 Nov 5;7:54-56.

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PRP每月科技新知 (2022/05/17)- PRP可用於皮膚再生(回春)

文章前言: 這是一篇討論PRP用來促進皮膚再生的文章

文章內容: 因為PRP具有高濃度的血小板，富含生長因子和生物活性蛋白質，從而刺激傷口癒合、血管新生和膠原蛋白生成，因此廣泛的用在面部整形手術。PRP最流行的使用方式是以皮膚注射和微針局部應用的形式進行面部皮膚年輕化(回春)。截至目前的臨床研究，皆支持使用PRP(注射和/或與微針結合)來促進皮膚年輕化。

文章結論: PRP可用於皮膚再生

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp

原文摘要:

Platelet-rich plasma (PRP) has gained popularity in facial plastic surgery because of its role in wound healing. PRP, having a higher concentration of platelets, allows for greater release of growth factors and biologically active proteins, which in turn activates the wound-healing cascade stimulating neoangiogenesis and collagen production. One of the most popular uses for PRP is for facial skin rejuvenation in the form of dermal injections and topical application during microneedling. The promising nature of PRP makes using it for injection and/or in conjunction with microneedling a good addition to any practice that deals with skin rejuvenation.

文章出處: Peng GL: Platelet-Rich Plasma for Skin Rejuvenation: Facts, Fiction, and Pearls for Practice. Facial Plast Surg Clin North Am 2019, 27(3):405-411.

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PRP每月科技新知 (2022/05/06)- PRP 已用於治療多種皮膚病 (痤瘡、脫髮、皮膚潰瘍、黃褐斑、色素沉著過度和燒傷…等)

這是一篇教學型的文章---    

中文摘要: 富含血小板血漿 (PRP) 是一種包含在少量血漿中的血小板濃縮物，由於其高濃度的生長因子，在過去十年中已成為治療與皮膚相關的不同疾病的最具希望的選擇。當PRP是自體來源時，可降低發生不良反應和輸血傳播感染的可能性，因此PRP是對患者的最佳和安全的治療方法。 PRP 已用於治療多種皮膚病，例如痤瘡、脫髮和皮膚潰瘍。PRP的用途還擴展到其他皮膚狀況，例如黃褐斑、色素沉著過度和燒傷，在這些情況下PRP可以刺激組織修復和再生。本文的目的是回顧 PRP 對不同皮膚病學改變的處理和治療。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp

原文摘要:

Platelet-rich plasma (PRP), a platelet concentrate contained in a small volume of plasma, has become a promising option in the last decade to treat different diseases related to the skin due to its high concentration of growth factors. When it is of autologous origin, it decreases the probability of suffering adverse reactions and transfusion-transmitted infections, thus it is an optimal and safe therapy for the patient. PRP has been used in the treatment of several dermatological conditions such as acne, alopecia, and skin ulcers. Its use has also extended to other skin conditions such as melasma, hyperpigmentation, and burns, where it stimulates tissue repair and regeneration. The purpose of this article is to review the management and treatment of different dermatological alterations with PRP.

文章出處:

Merchán WH, Gómez LA, Chasoy ME, Alfonso-Rodríguez CA, Muñoz AL. Platelet-rich plasma, a powerful tool in dermatology. J Tissue Eng Regen Med. 2019 May;13(5):892-901.

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PRP每月科技新知 (2022/04/28)- 異源性PRP凝膠能促進皮膚傷口癒合

研究目的：評估異源性富含血小板血漿 (PRP) 凝膠對於手術皮膚傷口癒合的潛力。

研究方法：使用一隻健康狗的血液用於製備生產異源性 PRP原料，將葡萄糖酸鈣添加到 PRP 中以形成凝膠。在六隻兔子身上進行了兩次手術切除，一次在背部中線的右側，另一次在左側。一側被隨機分配到使用局部生理溶液治療，另一側使用異源性PRP凝膠治療。術後 0、3、7、10、14 和 17 天進行臨床評估（體重、疼痛敏感性、顏色、水腫、充血、滲出、結痂和肉芽）和形態學評估。第17天進行組織學分析。

結果：除了第 10 天出現結痂外，臨床變異數在實驗組之間沒有顯著差異。在比較傷口面積的部分，使用PRP組明顯縮短傷口癒合的時間。組織學結果發現對照組和 PRP 治療組之間沒有顯著差異。

結論：異源性PRP凝膠能促進皮膚傷口癒合，無不良反應。

# 防疫新選擇，免出門安全購物：

KimClaire網頁購買: https://kimclaire.1shop.tw/prp

原文摘要:

PURPOSE: To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits.

METHODS: Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel. Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17th day.

RESULTS: With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group.

CONCLUSION: Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.

文章出處:

Abegao KG, Bracale BN, Delfim IG, Santos ES, Laposy CB, Nai GA, Giuffrida R, Nogueira RM: Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits. Acta Cir Bras 2015, 30(3):209-215.

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PRP每月科技新知 (2022/04/20)- PRP 對於乾癬和異位性皮膚炎(濕疹)安全有效

研究目的：研究富含血小板血漿 (PRP) 對於乾癬或異位性皮膚炎(濕疹)的臨床效果。

研究方法：在 53 個月的時間裡，使用自體PRP 治療總共 40 名發炎性皮膚病患者。使用自體血漿 (ACP) 雙注射器製備 5-6 ml PRP 並皮下注射。在治療後的第 3、6、9 和 12 週進行了追蹤。除了病變大小外，還計算了乾癬面積和嚴重程度指數 (PASI) 和異位性皮膚炎(濕疹)面積和嚴重程度指數 (EASI)。在 p≤0.05 的顯著意義上對數據進行統計學評估。

研究結果：共有 30 名患者接受了乾癬的治療。肘部區域是最常見的治療區域（17 例）。平均病變大小從 8.2 cm² 減少到 0.3 cm² (p<0.00001)。值得注意的是，80% 的患者在最後一次追蹤達到完全緩解 (PASI100)。其餘 20% 至少達到 PASI70。十名患者接受了異位性皮膚炎(濕疹)治療，其中 6 例在手臂上，4 例在腿部上。平均病變大小從 8 cm² 減少到 0.155 cm² (p<0.00001)。值得注意的是，50% 的患者在最後一次追蹤達到完全緩解 (EASI100)。另一半至少達到EASI70。在所有情況下，病變大小逐漸減小。未報告任何不良事件。

結論：此研究揭示了乾癬和異位性皮膚炎(濕疹)的令人鼓舞的結果。自體PRP治療對所有患者均安全有效。

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原文摘要:

Objective: In this study, our aim was to investigate the clinical effects of platelet-rich plasma (PRP) on the skin of patients suffering from plaque psoriasis or atopic dermatitis.

Methods: Over a period of 53 months, we treated a total of 40 patients for inflammatory skin diseases with PRP. All of these patients were included in this study; 5-6 ml of PRP were prepared with the autologous-conditioned plasma (ACP) double syringe and injected subdermally. Follow-ups were conducted at three, six, nine, and 12 weeks after treatment. Besides the lesion size, Psoriasis Area and Severity Index (PASI) and Eczema Area and Severity Index (EASI) were also calculated. Data were evaluated statistically at a significance level of p≤0.05.

Results: A total of 30 patients were treated for plaque psoriasis. The elbow area represented the most common area of treatment (17 cases). The average lesion size decreased from 8.2 cm² to 0.3 cm² (p<0.00001). Of note, 80% of all patients achieved complete remission (PASI100) at the last follow-up. The remaining 20% reached at least PASI70. Ten patients were treated for atopic dermatitis. In six cases, efflorescences on patients’ arms were treated, and in four cases, patients' legs were treated. The average lesion size decreased from 8 cm² to 0.155 cm² (p<0.00001). Notably, 50% of all patients achieved complete remission (EASI100) at the last follow-up. The other half reached at least EASI70. In all cases, the lesion size decreased progressively. No adverse events were reported.

Conclusion: Our study revealed encouraging results for both psoriasis and atopic dermatitis. The autologous treatment was safe and effective in all patients. Further studies are required to validate these initial findings.

文章出處:

Kauhl W, Pototschnig H, Paasch U (October 04, 2021) Can Platelet-Rich Plasma Reduce the Burden of Inflammatory Skin Diseases Such as Psoriasis and Atopic Dermatitis?. Cureus 13(10): e18472. DOI 10.7759/cureus.18472.