#Drug Formulation Development

[sauravchemicals]

Process Development Manufacturing | Saurav Chemicals

Saurav Chemicals provides extensive process development services, guaranteeing the creation of efficient, cost-effective, and scalable processes tailored to your drug production needs.

[renejix58]

CDMO contract Manufacturing Service Provider in the USA

Renejix Pharma Solutions is a CDMO contract manufacturing service provider in the USA, offering reliable and innovative contract manufacturing excellence. Feel free to contact us; we are here for you 24/7. Call us at +1 (631) 210-5235.

For more information, visit https://renejix.com/

[asrarblog]

AI and Machine Learning in Pharmaceutical Drug Development – Asrar Qureshi’s Blog Post #1102

[ussfgmp]

Are Botanical Extracts Safe for Drug Manufacturing?

As natural ingredients and herbal supplements gain popularity in the health and wellness space, many pharmaceutical companies are turning to botanical extracts as a source of drug manufacturing. But how safe are botanical extracts in pharmaceutical applications? This comprehensive guide will explore the safety, efficacy, and regulatory landscape surrounding botanical extracts in drug manufacturing.

1. What Are Botanical Extracts?

Botanical extracts are concentrated compounds derived from plants. They contain active ingredients that have been isolated or enhanced to retain the plant's therapeutic properties. These extracts are made through various methods, including water or alcohol extraction, distillation, or solvent extraction, which isolate specific bioactive compounds that offer potential health benefits.

2. Why Are Botanical Extracts Used in Drug Manufacturing?

Pharmaceutical companies have long sought natural sources for drug development, and botanical extracts are no exception. Plants offer a rich diversity of compounds, including alkaloids, flavonoids, and terpenes, which may have therapeutic effects. Many drugs have been derived from plant sources, like aspirin from willow bark and paclitaxel from the Pacific yew tree. Botanical extracts allow drug manufacturers to harness the benefits of these bioactive compounds in a controlled and concentrated form.

3. Common Botanical Extracts in Pharmaceuticals

Several botanical extracts are frequently used in drug manufacturing due to their therapeutic potential:

Curcumin from turmeric, is known for its anti-inflammatory and antioxidant properties.

Resveratrol from grapes has been studied for its potential heart health benefits.

Ginseng extract is often used for its adaptogenic and energy-boosting effects.

Sarapin from pitcher plants, is used for natural pain relief (more on Sarapin can be found at ussfgmp.com).

These examples demonstrate the diverse applications of botanical extracts in various therapeutic areas.

4. Are Botanical Extracts as Effective as Synthetic Drugs?

One common question about botanical extracts is whether they can match the potency and efficacy of synthetic drugs. Botanical extracts can be highly effective, but their effectiveness often depends on the dosage and concentration of active compounds. For instance, some botanical extracts may work best as part of a multi-component formulation or when used synergistically with other ingredients. However, the variability of botanical sources and extraction methods can lead to inconsistencies in their effectiveness.

5. Safety Considerations for Using Botanical Extracts in Pharmaceuticals

When considering the safety of botanical extracts, several factors come into play:

Source and Quality of Plant Material: The safety of an extract heavily depends on the source of the plant, as well as how it's cultivated and harvested.

Extraction Method: Different methods of extraction can influence the chemical profile and concentration of the final product.

Potential for Contamination: Botanical extracts can be contaminated with pesticides, heavy metals, or microbes if not sourced and processed carefully.

Allergic Reactions: Some individuals may experience allergic reactions to certain botanical extracts.

For safe use in pharmaceuticals, botanical extracts must meet stringent quality control standards.

6. Regulatory Standards for Botanical Extracts in Pharmaceuticals

In the United States, botanical drugs are regulated by the FDA under a specific guidance framework. Botanical drugs must undergo rigorous testing to demonstrate safety and efficacy. For example, botanical drugs must be evaluated through clinical trials to ensure they meet the same standards as synthetic drugs. The FDA's Botanical Drug Development Guidance outlines how companies can bring botanical drugs to market, including data requirements and quality assurance protocols.

7. Advantages of Botanical Extracts in Drug Development

The use of botanical extracts in pharmaceuticals offers several advantages:

Natural Composition: Botanical extracts are often perceived as safer due to their natural origin.

Sustainability: Many botanical sources are renewable, making them more sustainable than synthetic alternatives.

Lower Side Effects: Certain botanical extracts may have fewer side effects compared to synthetic drugs.

These benefits have spurred increased research into plant-based therapies and drugs.

8. Risks and Challenges of Botanical Extracts in Pharmaceuticals

While botanical extracts offer potential benefits, they also present challenges:

Consistency in Potency: Variability in plant sources can lead to inconsistencies in the active compounds of botanical extracts.

Standardization Issues: It's difficult to standardize botanical extracts due to natural variation in plant material.

Interactions with Other Drugs: Botanical extracts can interact with conventional drugs, leading to potential side effects or reduced efficacy.

Understanding and managing these risks is essential for the safe use of botanical extracts in pharmaceuticals.

9. Case Studies of Botanical Extracts in Modern Medicine

The following case studies highlight successful applications of botanical extracts in drug development:

Artemisinin for Malaria Treatment: Derived from the sweet wormwood plant, artemisinin has become a cornerstone in malaria treatment.

Paclitaxel for Cancer: Sourced from the Pacific yew tree, paclitaxel is an effective chemotherapy agent.

Cannabidiol (CBD): A non-psychoactive compound from cannabis, CBD is used to treat epilepsy and other conditions.

These examples showcase how botanical extracts have made a substantial impact on modern medicine.

10. The Future of Botanical Extracts in Drug Manufacturing

Advances in biotechnology and analytical chemistry are enabling better standardization, quality control, and bioavailability of botanical extracts. Additionally, research is ongoing to discover new plant sources with therapeutic potential, as well as to improve methods for extracting and purifying active compounds from plants. The integration of botanical extracts in pharmaceuticals is likely to grow as technology enhances their reliability and safety.

Conclusion

Botanical extracts represent a valuable resource for drug manufacturing, offering unique therapeutic benefits, sustainability, and a natural composition. However, the safety of botanical extracts in pharmaceuticals hinges on careful sourcing, rigorous testing, and adherence to regulatory standards. While botanical extracts offer great potential, they must be treated with the same care and scrutiny as any other drug component. As research continues to advance, the role of botanical extracts in pharmaceuticals will likely expand, helping to bridge the gap between traditional and modern medicine.

[colinwilson11]

Ophthalmic Drugs Contract Manufacturing: An Overview Of The Rapidly Evolving

Regulatory compliances play a pivotal role in ophthalmic drugs manufacturing due to stringent norms and quality standards set by regulatory bodies like US FDA, EMA, etc. Ophthalmic products manufacturing needs to adhere to Current Good Manufacturing Practices (CGMP) to ensure product safety, efficacy, and quality. Ophthalmic drugs contract manufacturers invest heavily in approvals, certifications, and manufacturing facilities upgradation to meet evolving regulatory guidelines. They focus on establishing robust quality management systems, validation protocols, change control systems, and document controls. Regular product quality reviews and internal audits also help contract manufacturers deliver regulatory compliance.

Leading Technology And Specialized Equipment

Ophthalmic drugs require highly sophisticated and precise manufacturing technologies and equipment due to small volumes and concentrations. Contract manufacturers leverage leading technologies like microprocessing, microfluidics, micro-molding, and precision coating to produce a diverse range of ophthalmic products. They invest in specialized, high-end equipment like micronizing mills, soft-gel encapsulation systems, and laser-marking machines. Automation and digitization using IoT, AI, and analytics also help boost production efficiency and quality. The technologies and equipment enable contract manufacturers to cater to customized packaging and dosing requirements of ophthalmic drugs.

Specialized Facilities And Cleanrooms 

Ophthalmic products demand stringent environmental control and hygiene standards owing to small dosage forms and direct exposure to eyes. Contract manufacturers operate highly specialized facilities with ISO classified cleanrooms to minimize microbial and particulate contaminations. Features like laminar air flow, differential air pressures, high-efficiency particulate air (HEPA) filtration help maintain critical environmental conditions. Periodic qualification and calibration of facilities and utility systems also ensure process validation. Designated areas for raw material receipts, products manufacturing, quality testing, packaging, and warehousing follow zoning principles. These specialized facilities enable contract manufacturers to ensure sterility, stability, and preserved efficacy of Ophthalmic Drugs Contract Manufacturing and storage.

Capabilities In Sterile Fill/Finish

A major portion of ophthalmic drugs require sterile fill/finish due to direct administration into eyes. Contract manufacturers have dedicated sterile suites equipped with barrier isolators, aseptic processing equipment, and self-contained environmental control systems. Technologies like lyophilization and terminal sterilization aid microbial decontamination. Stringent personnel training on garbing and cross-transfer procedures helps avoid contamination risks. Regular media fills and endotoxin challenge simulations validate sterilization process efficacy. Quality control testing through rapid microbiological methods, particulate testing and endotoxin assays ensure sterility assurance of aseptically filled ophthalmic products. These capabilities enable outsourcing of sterile fill/finish operations for preservative-free ophthalmic drugs.

Specialized Analytical Testing 

Ophthalmic drugs require meticulous analytical characterization and quality testing due to small amounts administered near eye region. Contract manufacturers invest in -leading analytical instruments like HPLC, GC, dissolution testing equipment, and particle size analyzers. Methods involve testing of identity, purity, content uniformity, particulate matters, pH, osmolarity, sterility, bacterial endotoxin, and preservative content. Stability indicating methods help real-time product monitoring on storage. Various ophthalmic dosage forms like ointments, gels, suspensions etc. also require formulations development and evaluation of rheological properties, spreadability and bioavailability. Contract testing laboratories employ highly trained analytical experts, validated methods and computerized data integrity systems. These specialized testing capabilities help ensure efficacy and safety of outsourced ophthalmic products.

Case Studies And Fill-Finish Agreement 

A leading UK-based ophthalmic drug firm outsourced development and fill-finish operations of its novel anti-inflammatory eye drop to a US-based contract manufacturer. Impressed by proven sterile fill/finish capabilities, quality systems, and regulatory compliance track record, six-month technology transfer was completed on schedule. Further, a 1-year commercial supply agreement was signed with production scale-up clauses. Another Ophthalmic Drugs Contract Manufacturing giant outsourced manufacturing of its portable multi-dose ophthalmic dispensers to a reputed Japanese contract manufacturer specializing in medical-device moulding. Leveraging expertise in micro-molding and precision assembly, the firm ensured precise dosing and improved patients' compliance. These cases illustrate effective collaborations aiding faster access of critical ophthalmic therapies. 

Get more insights on this topic: https://www.trendingwebwire.com/ophthalmic-drugs-contract-manufacturing-meeting-global-ophthalmic-medications-demands-through-specialized-services/

About Author:

Priya Pandey is a dynamic and passionate editor with over three years of expertise in content editing and proofreading. Holding a bachelor's degree in biotechnology, Priya has a knack for making the content engaging. Her diverse portfolio includes editing documents across different industries, including food and beverages, information and technology, healthcare, chemical and materials, etc. Priya's meticulous attention to detail and commitment to excellence make her an invaluable asset in the world of content creation and refinement. (LinkedIn - https://www.linkedin.com/in/priya-pandey-8417a8173/)

*Note: 1. Source: Coherent Market Insights, Public sources, Desk research 2. We have leveraged AI tools to mine information and compile it

[reasonsforhope]

"A cure for HIV could be a step closer after researchers found a new way to force the virus out of hiding inside human cells.

The virus’s ability to conceal itself inside certain white blood cells has been one of the main challenges for scientists looking for a cure. It means there is a reservoir of the HIV in the body, capable of reactivation, that neither the immune system nor drugs can tackle.

Now researchers from the Peter Doherty Institute for Infection and Immunity in Melbourne, have demonstrated a way to make the virus visible, paving the way to fully clear it from the body.

It is based on mRNA technology, which came to prominence during the Covid-19 pandemic when it was used in vaccines made by Moderna and Pfizer/BioNTech.

In a paper published in Nature Communications, the researchers have shown for the first time that mRNA can be delivered into the cells where HIV is hiding, by encasing it in a tiny, specially formulated fat bubble. The mRNA then instructs the cells to reveal the virus.

Globally, there are almost 40 million people living with HIV, who must take medication for the rest of their lives in order to suppress the virus and ensure they do not develop symptoms or transmit it. For many it remains deadly, with UNAids figures suggesting one person died of HIV every minute in 2023.

It was “previously thought impossible” to deliver mRNA to the type of white blood cell that is home to HIV, said Dr Paula Cevaal, research fellow at the Doherty Institute and co-first author of the study, because those cells did not take up the fat bubbles, or lipid nanoparticles (LNPs), used to carry it.

The team have developed a new type of LNP that those cells will accept, known as LNP X. She said: “Our hope is that this new nanoparticle design could be a new pathway to an HIV cure.”

When a colleague first presented test results at the lab’s weekly meeting, Cevaal said, they seemed too good to be true.

“We sent her back into the lab to repeat it, and she came back the next week with results that were equally good. So we had to believe it. And of course, since then, we’ve repeated it many, many, many more times.

“We were overwhelmed by how [much of a] night and day difference it was – from not working before, and then all of a sudden it was working. And all of us were just sitting gasping like, ‘wow’.”

Further research will be needed to determine whether revealing the virus is enough to allow the body’s immune system to deal with it, or whether the technology will need to be combined with other therapies to eliminate HIV from the body.

The study is laboratory based and was carried out in cells donated by HIV patients. The path to using the technology as part of a cure for patients is long, and would require successful tests in animals followed by safety trials in humans, likely to take years, before efficacy trials could even begin.

“In the field of biomedicine, many things eventually don’t make it into the clinic – that is the unfortunate truth; I don’t want to paint a prettier picture than what is the reality,” stressed Cevaal. “But in terms of specifically the field of HIV cure, we have never seen anything close to as good as what we are seeing, in terms of how well we are able to reveal this virus.

“So from that point of view, we’re very hopeful that we are also able to see this type of response in an animal, and that we could eventually do this in humans.”

Dr Michael Roche of the University of Melbourne and co-senior author of the research, said the discovery could have broader implications beyond HIV, with the relevant white blood cells also involved in other diseases including cancers.

Dr Jonathan Stoye, a retrovirologist and emeritus scientist at the Francis Crick Institute, who was not involved in the study, said the approach taken by the Melbourne team appeared be a major advance on existing strategies to force the virus out of hiding, but further studies would be needed to determine how best to kill it after that.

He added: “Ultimately, one big unknown remains. Do you need to eliminate the entire reservoir for success or just the major part? If just 10% of the latent reservoir survives will that be sufficient to seed new infection? Only time will tell.

“However, that does not detract from the significance of the current study, which represents a major potential advance in delivery of mRNA for therapeutic purposes to blood cells.”"

-via The Guardian, June 5, 2025

[frontropharma]

Advanced Product Development Solutions at FrontroPharma

At FrontroPharma, our contract research organization (CRO) services specialize in advanced product development, including Nano formulations and liposomes. We excel in optimizing drug delivery systems for complex generics, AYUSH products, nutraceuticals, and cosmetics. Our rigorous scientific approach ensures precision and efficacy in every formulation, transforming your concepts into groundbreaking products. Trust us to elevate your product development process with unparalleled expertise and innovation.

[aragenlifesciences]

Expert Pre formulation development

CDMO pharma companies specialize in comprehensive drug development services, ensuring seamless progression from pre formulation development to clinical trials. Their expertise includes solid form optimization, compound profiling, and tailored preclinical formulations to expedite drug development.

[chemxpertdatabase]

Contact Chemxpert Database to Top Pharma Companies in World

In the dynamic realm of pharmaceuticals, excipients stand as unsung heroes, playing pivotal roles in drug formulation. These pharmaceutical excipients, often overlooked, are crucial components ensuring stability, bioavailability, and efficacy of medications. Within the US pharmaceutical landscape, meticulous management of these excipients is paramount for ensuring quality and regulatory compliance. Pharma management strategies encompass rigorous oversight of excipient sourcing, testing, and integration into formulations. Among the top pharma companies globally, excipient selection and management are central pillars of their success, underpinning the development of groundbreaking medications. These industry leaders exemplify excellence in leveraging pharmaceutical excipients to innovate and address unmet medical needs worldwide.

[sentisspharma]

A niche pharmaceutical company committed to provide the best quality Ophthalmic, Inhalation (for nebulization), Otic and Nasal drops since its inception in 1990. Sentiss is recognized as a Great Place to Work in India for 7 years* in a row. Sentiss is committed to serve and build a stronger society through its social responsibility “SEVA” initiatives with main focus on health, education and environment.

https://sentisspharma.com/

[mimimigan]

Spideypoolverine Headcanons

Because I have an obsession

Also some of these might be slightly Deadpool and Wolverine (movie) specific

•Both Logan and Wade call Peter 'Petey' (or Petey-Pie sometimes in Wade's case) (Logan called Jean Jeannie in the comics so. He would def call Peter Petey)

•Peter and Wade can both safely sleep next to Logan despite his claws coming out sometimes (Wade because he can regenerate obviously and Peter because his spider sense works even in his sleep)

•Logan is slightly more successful than Wade at getting Peter to sleep/eat/rest properly. Mostly because Logan won't take no for answer and will try to wrestle the guy into taking a nap (even though Peter is stronger sometimes he just relents and lets Logan win)

•Speaking of Peter being stronger, it took Logan forever to realize this, (Post DP&W) especially since Peter pulls his punches while fighting. Logan could tell he was really fast and agile so he thought that was the main reason Spidey did so well. So the first time Spidey got buried by some rubble, Logan panics. He thinks there's no way Spidey isn't horribly injured. Probably goes a little crazy and gets a little too violent, ending whatever conflict it was before Spidey can unbury himself from the rubble. He is definitely not super clingy afterwards.

•Logan doesn't fully grasp how strong Spidey is until he like, catches a car going 60 mph or something. He does get an inkling of it though when Peter picks him up and carries him around like he weighs nothing. Wade can also pick Logan up too. He does not like that they can do this. He secretly enjoys it a lot.

•Wade and Logan are, of course, very protective of their mortal squishy boyfriend. This does mean that they jump in front of attacks that he would easily dodge with his Spider sense. Peter does not like this.

•Peter also does not like their wanton violence to each other. Especially since he's the one who usually cleans up the blood. But he really just doesn't enjoy either of them being injured. Even though he knows they will heal, it still bothers him.

•Logan and Wade fight over who gets to patch up Peter when he gets injured enough to need stitches, bandanging up, etc. both claiming they are better at it. In reality it doesn't matter which of them does it, as long as Peter doesn't do it himself, as he sucks at it.

•Logan loves that Peter and Wade are both yappers. He likes that at least one of them is probably talking, if not both of them talking rapidly together. He likes the noise even if he isn't listening, though he usually is.

•Wade is, of course, physically clingy. He's almost always touching one if not both of them. Holding their hand, his hand on their thigh, playing with their hair, pulling them to sit on his lap or sitting on their lap, etc. It becomes infectious to where the three of them are just constantly touching if they're home, probably still holding hands or in close proximity out in public.

•Peter takes many pictures of the other two, most of them candid. Pictures of Logan sleeping on the recliner like an old man, of Wade mid-laughing, of the two of them wrestling, etc.

•Wade is the money-maker, Logan cooks, and Peter keeps their place clean.

•Wade is the most classically 'romantic'. Buying flowers and gifts for the other two, planning fun dates, writing silly love notes, etc. He doesn't really care if he receives things in return, though of course he appreciates it, he just likes to give them things.

•It really bothers Peter how both of them are in chronic pain (which is borderline canon for both Wolvie and DP) and that they both struggle with mental health issues (PTSD for Logan and the voices along with other things for Wade) and tries to find ways to help them since he's smart enough to. Even if it's little things like heated blankets or specially formulated lotions, icy hot, etc. I imagine he would spend a lot of time trying to make them feel better, including trying to develop drugs that actually work with their healing factors.

I could keep going because I think about these three way too often but I'll end it here. Maybe I'll make another post later about more headcanons I have hahah.

[ohtobeleah]

Was It Over? // Jake Seresin

-> Chapter Twelve: [Bring Me The Horizon]

Summary: After Jensen and Jake finish their face-off, you tell Jake how it is. He practices the art of holding himself accountable for his actions, and you get a call that would send you into a downward spiral. Putting you in jeopardy right before your surgery.

Warnings: MAIN CHARACTER DEATH Sick!reader. Breast cancer diagnosis. Jake Seresin x F!reader. Angst, hospital & medical inaccuracies. SLOW BURN ROMANCE/ Inaccurate medical information. Relationship turmoil. Mentions of religion. JEALOUS JAKE!

Word Count: 5.1k

Author Note: WOW and we’re back baby. Another year has passed us by and we are finishing this series. Strap in for the final three episodes of this roller coaster of a series. You never know where we might just end up.

Series Masterlist | Main Masterlist

The triangular theory of love is a theory developed by Robert Sternberg. In the context of interpersonal relationships, the three components of love according to the triangular theory are, intimacy, passion, and a decision/commitment component. For Jake, he felt like love was more than just a triangular theory. Love is all-encompassing in every aspect of life. And if you look closely, love can be seen all around us in all different forms. 

“Bradshaw, now’s not the time man,” Jake sighed as he made his way back down the hospital hallway to your room, walking with his shoulders down and his ego battered and bruised. “I gotta fix an issue and my head’s not–” Before Jake could finish his sentence, Rooster interrupted with a crucial question he needed answered now. 

“How is your mother gonna react when I show up to take your kids?” The genuine concern in Bradley’s voice was clear enough for Jake to stay on the line as he walked. “Do I need to be concerned about her calling the cops?” 

“Dude,” Jake groaned to himself in frustration as he walked with purpose down the hall. He could feel his rage and frustration bubbling to the surface with every step he took. Who did this Jensen guy think he was? Who did Jake think he was? You didn’t ask for any of this and you definitely didn’t deserve his tantrum before. “She’s not gonna call the cops on you, just tell her to call me if you have any issues and get Jas to help you.” 

“Your mother is a terrifying woman–” Rooster added, just to rub salt into Jake’s already existing childhood wounds. 

“Well, at least she’s alive.” Jake had never regretted a sentence more in his life. The second he realised what he’d said and who he was speaking to, his heart fell straight out of his arse. “Bradshaw I didn’t mean–” Before Jake had a chance to speak, before he had a chance to explain that he wasn’t talking about Carole—he was talking about you—the line went dead. 

“Fuck!” Jake looked at his phone screen to see the call had indeed been ended. He felt the panic inside his chest. The guilt. 

Jake had developed a pretty uncanny ability to fuck things up. He couldn’t help himself at the moment. He was having a hard time regulating his emotions. It never had been his strong suit, but now it was worse than ever. Perhaps the idea of losing you forever was the cause. Jake couldn’t accept the possibility that there was a good to fair chance you might not walk away from this fight. 

But he could see the bottom of your bed again and where your feet were underneath the breathable hospital blanket that did nothing to keep you warm. With every step Jake took in the hall, he got closer and closer to where he’d left you. And with every step he took he tried to formulate an apology that would reflect his utter sorrow. His pain. His guilt. You were the first person that deserved an apology for Jake’s actions. Bradley would just have to wait. 

In hospitals, people see addiction every day. It’s shocking how many kinds of addiction exist. It would be far too easy if it were just drugs, alcohol and cigarettes people were addicted to. The hardest part about kicking an addiction is wanting to kick it. I mean we get addicted for a reason, right?

Often, too often, things start as a normal part of your life and at some point, cross the line into obsessive needs, compulsive out of out-of-control desires. It’s the high people chase. The high that makes everything else…fade away. But nothing could make Jake Seresin fade away. Nothing so far had helped you kick the addiction. Not even when you left. 

The harsh reality about addiction is it never ends well. Because eventually, whatever it is that was getting you that high…stops feeling good and starts to hurt. So when the very person you were addicted to walked back into your hospital room, the hurt started all over again well and truly after the high had died down. 

“Okay,” Jake stopped in his tracks when he saw that look in your eyes. Although a sense of calm washed over him the second he saw you again, he knew he was probably the last person you wanted to see after the way he behaved before. “I recognised I’m in the wrong here.” It was the look he wished he’d never been on the receiving end of again. A look of disappointment mixed with anger, hatred, and, above all, sadness. “I owe you an apology.” 

“No thanks,” The tone you conveyed was sinister. There was no warmth. No love. No room for compassion or empathy. “I’m not interested in another empty apology.” You didn't even look Jake’s way. You just continued on with the little word finder you had picked up in Jensen and Jake’s absence. “If you’re done swinging your dick around in the hall like a psychopath, I need to rest.” 

Jake was stunned. He wasn’t sure how to respond. He knew he deserved that. He knew he’d taken things too far. He knew he’d flown over the handlebars the second Jensen stepped into your room. But it was all out of fear of losing you. Why couldn’t you see that? Jake felt as if he could stand right in front of you and you’d look straight through him. 

“Honey, don’t be like that,” Jake sighed as he took a few steps towards your bedside. The bouquet Jensen had brought you was lying haphazardly on the little bedside cupboard. “I shouldn’t have acted the way I did alright, I know, and I promise you I’m trying here.” Jake sighed as he looked at the flowers another man had gone out of his way to buy you. A dying man. 

A dead man walking. 

“I’m glad you’re self-aware,” You replied yet again without paying any mind to Jake’s whereabouts in your room. “Never really was your strong suit.” You murmured just loud enough for Jake to hear your mild insult. You could have said it louder, hell, you should have. But regardless of Jake’s actions…he was an addictive drug. One that made it incredibly hard to kick the habit. One you knew you’d go back to. One you wished loved you the same way you loved him. 

“Jensen knocked some sense into me,” Jake tried his best to explain all the while he tried to hold back the lump in his throat. The kind of lump that brought tears and panic attacks with it. “He was kind enough to set the record straight.” 

“You understand how big of a piece of shit you have to be to say what you said, right?” It was the first time your tone had changed. There was something beyond painful in the way you spoke. Something Jake had tried time and time again not to be the cause of. Heartbreak. Betrayal even. “You jealous, insecure man.” 

For a second, Jake absorbed the blow. He took it on the chin like a champion because nothing you were saying was wrong. He was scared of losing the love of his life in a way that wasn’t just through marriage. 

“I know,” Jake replied as he picked up the bouquet and looked around. He was looking for somewhere to put them. Somewhere to display the beauty that they emulated. Beauty that reflected you. Where could he get a jug or something to put these in? “I don’t have an excuse to give you, honey,” Jake was trying his best to stick to the honesty is the best policy crap he’d been taught as a child. “I’m just, I got so worked up when he came in here knowing more about you than I did and–” 

“Jensen is a friend from group therapy,” You explained yet again. “And this is the last time I’m gonna say it before I let you run off with whatever version of a warped reality you wanna believe but I swear, there isn’t anyone else.” You sat up a little straighter in your bed as you closed your find-a-word book. “I have cancer, Jake. In what world would anyone want me?” 

“You love her, don’t you? You’re in love with my wife, say it.” Jake could hear himself asking the all-important question. He could see himself standing in the hall with the man who had selflessly intertwined his life with yours in order to make you feel less alone in the world.

“Maybe–” Jake vividly heard Jensens reply as he stood staring at the tears that fell down your cheeks. Tears he was the very cause of. “Maybe I love her, but I don’t get a chance to explore that, you do though.” 

“Me?” It was the sincerity in Jake’s voice that broke you as he put the bouquet down where he’d originally found it and made his way to sit beside you on your bed. “I want you forever and the very idea that some guy—some guy I know is a better man than I will ever be—brought you, my wife, flowers when I didn’t? Kills me.” 

“I don’t care about the flowers,” You sighed as Jake wrapped you up in his arms. 

“I know, but I let my own insecurities out in a way I never should have and I’m so sorry for the things I said. You don’t deserve that, ever.” It was genuine. The apology for his actions. Jake knew he was in the wrong with how he acted out. You could very clearly see that he was truly sorry. But letting him know that now would have been too easy. 

The pair of you sat in your hospital bed for the better half of five minutes silently enjoying each other’s presence and gentle touch. Until you broke. 

“I hope you aren’t expecting me to say apology accepted?” You smiled softly as you nuzzled into Jake’s chest a little more. Finding a comfortable position to rest in as you sunk lower in the hospital bed that wasn’t built for two fully grown human beings. 

“Nope,” Jake chuckled as he kissed the top of your head, freshly shaved and matching his own. “And I don’t think Rooster is gonna be accepting any apology I give him anytime soon, so the list is growing.” 

“What happened with Rooster?” You asked cautiously. You felt the tension take over your husband’s body as he processed what you‘d just asked. Jake held you a little tighter as he once again kissed the top of your freshly shaved head. The notes of your shampoo must have seeped into your scalp. He could still vividly smell the residual fragrance of bergamot. 

“Nothing that can’t be explained,” Jake sighed softly. He hated himself with a deep-rooted passion right now. The one thing he wanted to do more than anything else was take you home and pretend everything was alright for just one night. But he couldn’t do that. “Please, don’t worry about my mistakes when you have so much on your plate already. I’ll figure it out. Rooster and I always do.” 

“Hmm,” You replied with hesitation as you let your eyes close. The lup-dup of Jake’s heartrate soothed your soul, a heart that begged to be loved in all the ways he deserved to be loved. “I hope so. Martha Stewart’s best and closest friend said the words that sent her to prison,” You explained all the while Jake’s fingers drew unidentifiable objects into the supple skin of your forearm, careful not to mess with any of the tubes attached to you. It was grounding. “So whatever happened between you and Bradshaw I hope you fix it before he spills all your dirty little military secrets.” 

“Good thing you’re a true crime author, huh?” Jake smiled with a reluctance to give in to his biggest desire. What would be the repercussions of sneaking you out of this hospital right now? 

“No way you’re ending up in one of my books anytime soon,” You giggled softly. Jake felt his heart skip a beat at the sound of your happiness. You felt it too. The moment his heart decided it needed a second to process the laughter you let out. “I love you.” 

“I love you too, honey,” Although the circumstances said otherwise, for a second Jake felt like the luckiest guy on the planet. To be loved by you. To be the one you called home. To be the man you chose would forever be Jake’s greatest accomplishment in life. “I could live a thousand lifetimes and not deserve you in any of them.” 

************************

“No signs of life.” 

There’s a reason surgeons learn to wield scalpels. They like to pretend they’re hard, cold scientists. They like to pretend they’re fearless. But the truth is they become surgeons because somewhere, deep down, they think they can cut away that which haunts us. 

Weakness, frailty, death. 

It isn’t just surgeons. It’s paramedics too. First responders like to think they have a hand in what fate holds for you. They believe that if they can move a little quicker, stop the bleeding a little faster, and save you from life-threatening injuries, they can give you extra time. Even for just a day. 

But the truth is, we don’t know a single person who isn’t haunted by something…or someone. And whether people try to slice the pain away with a scalpel, pull someone from a car wreck, or shove it in the back of a closet…our efforts usually fail. 

So the only way we can clear out the cobwebs is to turn a new page or put an old story to rest…finally, finally…to rest. 

“Do we have any information on the victim?” Ilona shook her head as her unit chief looked around the scene. A single-car accident. The mangled wreck of twisted aluminium and shattered glass really painted a telling tale of destruction. No one could have survived the magnitude of injuries that they would have sustained on impact. 

“I’ll see if one of the guys picked up a phone or something, perhaps it’s still viable,” Ilona replied in a monotone voice. She was new to the horrors that her job sometimes brought with it. The hurricane forced winds that would sometimes knock her right off her feet. But she was learning how to work through the plethora of different emotions in a professional manner. “Poor guy, what do you think happened?” 

The black body bag laid out on the stretcher was a not-so-gentle reminder that life was a precious gift. Paramedics carried the deceased man until they were loading him into the back of the van. No sirens would be needed for this particular patient. 

“Crash investigation should have a report for us in about a week,” Taylor, the unit chief, replied as he watched the doors to the ambulance close with a thud. “We’ll need to get an ID as soon as possible to inform the family.” 

“I have a phone!!” Ilona heard the explanation from one of her co-workers. She turned with a grin on her face back to her boss. 

“We have a phone,” She repeated. “I’ll get right on it chef.” 

************************

Lydia still felt awful. She hadn’t quite shaken the existential dread that came with her almost career-ending mistake. She sat behind the desk at the nurses station just replaying the events back in her mind. How could she just assume someone to be someone’s emergency contact? 

In retrospect, Lydia now understood clear as daylight that relationships were interpersonal and held deeper value than what was on paper. Jake Seresin may not have been your emergency contact, but he sure was the love of your life. 

“You want me to do her observations?” Lydia heard one of her colleagues ask with a snicker. They all knew by this point in time what she had done. But now wasn’t the time to retreat and fall back. If she wanted to get past this, she needed to put on a brave face. 

“Nope,” Lydia shook her head as he rose to her feet. “I’ve got it,” She explained with a faux smile. “Is he not intimidating?” 

“Mr. Seresin?” Her colleague, Rebecca, replied with a mouthful of two-minute noodles she’d been scoffing down in the small window of reprieve. “Guy’s like a labrador. I mean, that is if you didn’t fuck up his wife’s chart badly enough to call the wrong person labelled clearly as an emergency contact.” 

“Ha. Ha.” Lydia barely had the time or the energy to humour her coworkers as they giggled and snickered as she made her way into your room. Jake was wide awake. He hadn’t noticed Lydia standing in the threshold of the door. But Lydia noticed how he held you with such care as you slept soundly. 

“Oh,” She cooed softly as she knocked her knuckles against the door. “Sorry to interrupt Mr. Seresin but I need to take some observations for Dr. Ignati.” 

“Would it be alright if she slept for even just five more minutes?” Jake was quick to advocate on your behalf. He could tell just how tired and overwhelmed with everything you’d become. Now that he was here and by your side, he was going to make sure you got the best possible treatment there was to offer. 

Lydia looked around at the Christmas lights that hung around your room. They twinkled and changed colour on a whim, never staying the same colour for long. The way they hung like they’d been thrown all over the place added a joyful smile across her face. 

“I’m sure I can take my time,” Lydia agreed as she approached your bedside. “How’s she been feeling?” 

“As good as you’d expect to feel given the circumstances, kid,” Jake replied with a yawn. He was able to stretch his limbs a little as he did so. Although, with every move he cautiously made, Jake was careful not to disturb you. “I assume her surgery is still going ahead as planned?” 

“As far as I’m aware,” Lydia confirmed as she checked over your chart and wrote down what she saw on your monitors. “Dr. Ignati should be around earlier in the morning to discuss everything once more.” 

“How long have you been working here?” Jake asked. He thought some small talk would be beneficial for not only him but for Lydia too. He could tell the question struck a nerve but pressed on nevertheless. “Because if I’m being perfectly honest, it doesn’t seem like very long.” 

“Eight months now,” Lydia replied with a short tone. Jake chuckled to himself as he watched her cross out what she had previously written and wrote down what she had originally intended. “Can you tell I’m also not very good at my job?” 

“It doesn’t seem out of ignorance,” Jake offered up his version of advice. “I just think you’re rushing things.” The advice didn’t fall on deaf ears like Jake thought it might. Instead, Lydia stopped what she was doing and took a nice deep long breath in. “In my line of work, things can go wrong in the blink of an eye,” Jake explained as he gently rubbed small circles into the palm of your hand with his thumb. If he was careful, he could feel the throbbing of your pulse point. “Gotta learn how to trust your instincts.” 

“My last instinct was that you were your wife’s emergency contact,” Lydia didn’t feel as if she needed to remind Jake of her biggest mistake of all time, but she did anyway. Jake understood what it was like to stay hung up on mistakes he couldn’t change. But he knew what he was about to say next would ease the burden Lydia was feeling. 

“Honestly, that’s more on me than it is on you,” Jake sighed as you stirred in his arms. He hoped you‘d stay asleep for just a little longer. You needed it. You deserved to rest soundly. 

“How so?” Lydia asked softly as went back to her duties. What Jake said to her next, however, had Lydia looking at the situation she found herself in from a different perspective. And sometimes a little perspective is all you need. 

“Well, I shouldn’t have let it get to a point where I wasn’t.” 

************************

Life gets easier when you realise that there’s no such thing as a wrong decision. Decisions are simply pivots. Each one you make will take you down a new road that will likely be both magic and miserable. Beautiful and ugly. That’s because life is a polarity. Easy doesn’t exist without hard. Good doesn’t exist without bad. 

There is no right or wrong answer. Trust that whatever decision you make can always be followed by another decision. 

“I hate this,” You mumbled as you walked to the bathroom with your IV support poll. “I already hate all of this so much.” You knew you were feeding into the depression, but you couldn’t help but feel down in the dumps about your current situation. 

“I think everything you’re feeling is valid, honey,” Jake answered as he trailed behind, giving you the independence he knew you wanted to keep but also staying close by in case you needed him. “After surgery–” Jake couldn’t even finish the sentence he’d planned to speak into existence.

“Don’t mention surgery,” You interrupted as you made your way over to the shower. “I don’t even wanna think about the surgery tomorrow.” 

“You do know that even if you ignore it, you still have to have surgery?” Jake asked as he frowned his brows and looked at you through the mirror. “We’re on the same page about that, right?” 

“What if I told you I’m just scared and don’t want anything to happen while I’m cut open like a fish?” You sassed as you looked back at Jake through the mirror. “If something happens–” 

“Hey, nothing's gonna happen,” Jake was quick to close the small gap between the two of you. He stood so close that your back pressed up against his chest. His eyes never left yours through the mirror you stood before. “I won’t let anything happen to you, or the kids for that matter.” 

“You left the kids with your mother,” You reminded your husband as he pressed his lips into a tight line of regret. “That alone is gonna cause them enough trauma Mr. ‘I won’t let anything happen to the kids’”

“You wound me, sweetheart,” Jake sighed as he tilted his head back and let out a sigh. “Besides, it’s actually you she hates, not the kids,” 

“Do you wanna help me shower or not?” You finally turned around to stand face-to-face with Jake. He was already smirking ear to ear. And it didn’t take long at all before his hands were dropping to your waist, slowly but surely making their slightly calloused way to the exposed skin of your ass. The hospital gown left little to the imagination. Jake wasn’t complaining. 

“When have I ever passed up the opportunity to see you naked?” Jake replied with a smirk you recognised was laced with lust. You felt him squeeze softly at your arse cheek playfully. “You’re the most beautiful woman I’ve ever laid my eyes on,” 

“You can’t help yourself, can you?” You giggled as Jake made sure to count the staples on your head. He knew whatever came next would have to be soft. It would have to be gentle and full of love. Not that it ever wasn’t full of love. 

“You drive me crazy, honey,” Jake explained as he caught your lips with his. You couldn’t help but to melt into his body as you kissed him back. Jake’s hands kneaded at the handfuls of arse he never wanted to let go of as he moaned into your mouth. 

The feeling was electric. The moan your husband let out sent shivers down your spine. It was an animalistic attraction that you’d tried so hard to suppress in your separation. But no toy on earth could satisfy you like Jake could. Not even when they were bought with his money. 

“I love you so much,” Jake cooed as he pulled back just enough to let his forehead rest against yours. “You’re gonna get through this, I promise,” He left it at that as you wrapped your arms around his waist for a much-needed hug. 

When you let go, you took a moment to drink in the sight of your husband. You stared at all the perfect little imperfections that littered his skin. The bags under his eyes were more prominent than normal. That was to be expected though. 

“Jake?” You cooed as you reached up to swipe the pad of your thumb across his lower lip. 

“I’m right here,” Jake replied as he kept his eyes closed. If he opened them he knew the tears he was trying so desperately to keep in would fall. If he cried in front of you he'd never be able to stop himself. 

“I need you to wake up for me, alright?” 

“What did you say?” Jake frowned as he opened his eyes and looked at you all confused. He knew what you said. He heard it crystal clear. But he needed you to repeat it. He needed to hear you say it again, just once. The fluorescent bathroom light flickered as he stared at you. For a second…Jake stood alone in a hospital room he didn’t recognise. Only for him to find himself standing right before you again in the blink of an eye. “Honey?” 

“I said I need you to get my phone for me?” You repeated calmly. “My phone is ringing and if I try to race to it I won't get there in time.” The explanation fit the response you gave him. Jake thought for a moment there he was going crazy. He needed more sleep, that was without a doubt the answer to his second of madness. 

Jake silently nodded in agreement before he kissed your forehead. He was only gone for a few seconds before he returned with your phone. Still ringing. 

“Who is it?” You asked as you reached out to take your phone from Jake. By the look smeared across his face, it wasn't someone he necessarily liked. 

“It’s your boyfriend,” Jake teased as he handed you your phone. Based on that comment alone you knew it was Jensen. 

“Careful Seresin,” You teased lovingly, hoping Jake believed what you had told him earlier. “He might be my next husband if you don't play your cards right.” 

Jake knew you were kidding and if anything he deserved that comeback. He didn't like the guy, that much was true. But he could see how having someone who knew what it was like to not know your own body could be beneficial to the mind. 

“You caught me at a bad time,” Jake watched as you answered the phone and held it up to your ear. “I–oh,” You paused. The immediate worry that took over the expanse of your face was enough to have the little hairs on the back of Jake's neck standing on edge. 

“Jensen Huges–” The name hung heavy in the air around you as you listened to the woman on the other end of the line. According to her, there had been a small piece of paper tucked into the phone case of this particular phone at the scene of an accident. Your name. Your number. “Oh, no, no don't tell me that,” You begged. 

Jake was unable to hear what was being said in the silence, but as he watched your eyes well with tears, he knew. 

“Y/n, give me the phone, honey,” He whispered as he gestured to your phone. You shook your head in response as you continued to listen to the woman who had introduced herself as Ilona. A police officer with the Rhode Island police. “Sweetheart?” Jake cooed as he reached out for you. The second his fingertips grazed your shoulder…You fell. “Shit–” 

“NOOOOOOO!” The guttural scream you let out scared Jake to his very core. He’d never in his life heard you sound the way you did as you crumbled on the floor in the bathroom. “NOOOOOO!” 

“Baby, baby I'm here, what happened?” Jake asked softly as he cupped your face in his hands. Your phone was long forgotten. It had crashed down when you did. “Tell me what happened so I can help you, honey?” 

“He–he left me,” You managed to gasp out between sobs. “And I can’t–I can’t breathe, Jake, I ca–” 

“Hey, I’ve got you,” Jake promised you as he held you tightly in his arms. “It’s gonna be alright,” Jake seemed to have been in the business of making promises he wasn't sure he'd be able to keep. He had no fucking idea if things would be alright. But he needed them to be. 

“I can't do this without him,” You cried out in utter anguish. “He, he was the st-strongest,” Jake could tell where this was going as you struggled to speak through your grief-stricken sobs. “If he—then I,” 

“I’ve lost count of all the rooms you've been tall in Y/n,” It broke his heart to see you like this. So full of heartbreak. So full of hopelessness. “You gotta keep fighting, I don’t know what I’ll do without you,” 

“I don't know how to!” You admitted to not only yourself but to your husband as well. “I don't know how!” 

“I’m right here, honey,” Jake held you as tight as he could on the bathroom floor. “You don't have a choice,” He explained as you cried your heart out in a way Jake had never seen you cry before. He could hear your voice echoing in his head. 

“You need to wake up,”

“You need to wake up,”

“You need to wake up,”

“Don't give up on me now Y/n, I can't lose you to this alright?” Jake felt his own tears streaming down his cheeks. The tears he no longer had the strength to fight. The way you willed yourself to wake up from this nightmare you were living broke his heart into pieces. The tears he no longer had the strength to fight. “Please, you gotta keep fighting this.” 

“He was the stronger one,” You replied as you cried yourself into a ball in Jake's arms. Clutching at his arms as your nails dug into his forearms. Grief was a funny thing. “I can't–” 

“You have to,” Jake cried with you. He was losing the love of his life and there was nothing he could do to stop this nightmare from happening. “I’m gonna be right here every step.” 

“I just want you to wake up,” It was all you said. Jake knew he heard you that time. He heard you loud and clear… “Please, don't leave me,” 

But he had no idea what you were saying. 

**********************************

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[renejix58]

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[scariusaquarius]

rehab. 35.

Avenger! Bucky Barnes x Winter Soldier! Fem! Reader

Summary: While on a mission to find any more possible super soldiers that were a part of the Winter Soldier program, Steve and Bucky make a discovery in an abandoned HYDRA base that was cleared out a few years prior to their mission. They discover the Reader, a long-forgotten soldier that was still asleep within a functioning cryostasis pod; still awaiting orders. While Bucky isn't happy about it, he is put up to the challenge of helping to rehabilitate the soldier in Wakanda where she may be able to become a person again.

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A/n: did y'all like that last little part of the previous chapter? heheheh it's beginning. this is a very short and sweet chapter for some comedic relief from the heaviness of the last few chapters, but still contains a bit of important development. <3 Also, if you enjoy my work, please consider buying me a coffee! If you would prefer to read Rehab on Archive, you may do so right HERE!

This is an au where Bucky joined the avengers but still rehabilitated in Wakanda (sometime before Infinity War [canon divergent cause NOPE]). I am NOT fluent in Russian, so I did use google translate cause I couldn't find a good translator that I trusted. If anything is wrong, PLEASE let me know!! Also, I tried to list as many warnings as possible so you know what the story will contain as chapters are posted. Stay safe!

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Genre: Slowburn, Enemies to Lovers/Friends to Lovers, Angst, Hurt/Comfort, Fluff, Humor, Drama, Dark Content Rated: Explicit Warning: Angst, Dark Content: Graphic Depictions of Sexual Assault, Blood and Gore, Mentions of Manipulation, Kidnapping, Canon-Typical Violence, Body Horror, Nonconsensual Body Modification/Scarring, Emotional and Physical Abuse, Mentions of Murder, Mentions of Suicidal Thoughts/Ideation, Graphic Depictions of Human Remains, Mentions of Sexual Coercion/Manipulation, Death, Misuse of Drugs/Forced Drugging, Self-Harm (Graphic Depictions and Mentions), Nightmares

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Author: ScariusAquarius

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rehab masterlist. / rehab masterlist 2. chapter 34

When the Avengers returned to Wakanda again, everyone was in a state of bittersweet triumph. The case had been won, the world was aware, and now it was a battle of public scrutiny.

Tony and Steve were walking side by side, both of them quiet and shoulders tensed. Neither of them had said a single word since embarking on the journey back to Wakanda, too angry and distraught by the trial to formulate proper thought.

Clint had managed to calm Wanda down, but due to the amount of people that had been thinking so strongly in the room, Wanda had become nauseous and had gotten a massive migraine. Maria had stayed behind in the US to deal with anymore legal fallout, and Natasha had been stone-faced the whole way; playing with a knife as she spaced out.

Shuri had been the only one out of the group to keep her head held high, ready and determined to begin rooting out the rest of HYDRA. When the aircraft had landed back in Wakanda, Shuri and the Avengers wasted no time in getting to Shuri's lab to begin planning.

However, as the group made it into Shuri's lab, they were surprised to see Sam and Bucky both sitting with (Y/n) as they watched a TV show.

Steve didn't know what it was, his eyes squinting, but Natasha smirked as she recognized the show. Currently, Bucky and Sam seemed to be in a semi-heated debate, Bucky gesturing to the TV with a large frown.

"Oh, come on! Salt rounds wouldn't work like that."

Bucky shook his head, adding with a frown as he watched Dean Winchester blow a door straight off of the hinges with his sawed-off shotgun.

"He would have dislocated his shoulder unless he was enhanced."

Sam gave Bucky an exasperated look, gesturing to the screen as well.

"Dude, it's fiction. You're not supposed to be critiquing and fact-checking the demon hunters."

Bucky pressed more, looking as though he was genuinely stressed out.

"Listen, you go clearing a room like that, and you're asking for trouble."

(Y/n), who had been sitting quietly and observing with a curious and calculating look, said quietly with a serious tone to her voice.

"No perimeter established. Tactics are flawed. The target could have slipped out through the window. It was open the whole time."

Sam gave (Y/n) a raised eyebrow as he crossed his arms.

"Oh, you're gonna critique a fictional show too?"

(Y/n) gave Sam a serious look, tilting her head a bit as Bucky smirked triumphantly at Sam from over her shoulder.

"It was sloppy. With no perimeter established and poor tactic execution, there will never be success in the mission. They must do better."

Sam grumbled to himself, and Natasha smirked at Steve as the man gave the woman a look of surprise.

"Looks like she's learning how to relax one Winchester takedown at a time."

Steve's lips twitched slightly, the amusement steady but half-hearted. He watched as Natasha plopped down beside (Y/n), asking her as Natasha glanced at the screen.

"What are you nerds watching, (Y/n)?"

(Y/n) glanced at Bucky and Sam for a moment, almost unsure, before looking back at Natasha with a shy look on her face.

"it is Supernatural...about two brothers that hunt monsters and...save people. It is sloppy."

Natasha smirked widely, amused by the seriousness in (Y/n)'s voice, and Steve sat down next to Bucky, who looked at the man with a silent but knowing look. Steve didn't have to say anything, Bucky nodding to him quietly.

I've got you back.

I know you do.

Tony groaned as he let his head fall back and rolled his eyes.

"Seriously? This is what you settled on? Have a little dignity, would you?"

Shuri just sighed, shaking her head as she quietly watched from the back. From just the small amount of time that the team had been gone, it seemed that Bucky and Sam had been able to jump forward in progress with (Y/n). While the woman still seemed to be shy, it was apparent that she was slowly opening up.

While using a show wasn't the most conventual way to make (Y/n) comfortable, Shuri had to admit that the fact (Y/n) was adding critique so freely was a good sign. Quietly, as the show continued and the group kept watching, Shuri began to work in her corner of the lab.

Sam began to try to explain the plot of Supernatural to Tony, but Tony didn't seem to be interested; already set with his opinion that the show was horrible. Natasha and (Y/n) stayed quiet as they continued to watch, and Steve raised his brow at the show with a skeptic look on his face.

"So...a TV show, huh?"

Bucky stated with a half-hearted annoyed look on his face.

"It wasn't my idea. Sam thought it'd be good to introduce (Y/n) to the latest pop culture, and she seems to enjoying it."

Steve joked quietly although the exhaustion and distraught demeanor was apparent.

"More like critiquing."

"She's making good points."

Bucky shrugged, and then the man became quiet again. Quietly, he spoke to Steve, saying as Sam began to bicker with Tony in the background much louder.

"Dr. Raynor called. Said she saw the whole trial and she wants to pick up (Y/n)'s case. She's gonna get in touch with you and Tony soon."

Steve looked surprised before he turned back to the screen and watched with a wince as the scene became a bit grotesque from the gore.

"I imagine that you're not very happy about it."

Bucky was quiet for a moment, glancing over at (Y/n) subtly, and he was almost surprised to see (Y/n) eyeing him quietly as well. Her gaze, however, was simply neutral; just listening before becoming distracted by Natasha beginning to critique and ask (Y/n) her opinions on the combat techniques. Bucky glanced at Steve, murmuring quietly.

"As much as I hate the idea of Raynor trying to help, she did make some pretty good points. Unless (Y/n) is receiving professional help with her rehabilitation, she'll have a harder time adapting to society when the time comes."

Steve nodded thoughtfully before he said comfortingly.

"I know Dr. Raynor is abrasive, but I think this is going to be good for (Y/n) in the long run."

Bucky stayed quiet, mulling over Steve's words before Steve added with another grimace as he continued watching the show as the over-the-top action sequence continued.

"You know, I might have to agree with Tony on this one."

Suddenly, (Y/n) spoke up again, the group regarding her with serious looks as they actively listened, (Y/n)'s eyes firmly on the screen as she pursed her lips into a small line.

"The tall one-he should have double-checked his exits. There's too much backlighting. He would have been shot before he made it to the stairs."

Tony exclaimed, gesturing to the screen as he agreed.

"See, she gets it. The shows a joke. You don't just walk into a haunted house like it's an everyday trip to Costco."

Sam gave Tony an amused look, leaning back in his seat.

"You say that as if you've been to haunted houses and a Costco."

Tony deadpanned slightly, giving Sam a look.

"I've been to both. One is full of ghosts, and the other is a haunted house."

Natasha snorted while Bucky and Steve gave Tony a confused look, and Sam just shook his head. (Y/n) looked unsure of what to do before glancing at Bucky as he spoke.

"She's not wrong. I'd take her over Dean Winchester in a fight any day."

(Y/n) then frowned, speaking as seriously as she could.

"That is not a fair comparison. He makes too much noise."

Steve couldn't help but to laugh gently, and Tony raised his hands in mock-surrender.

"I rest my case."

As the show continued on and the group continued to bicker and critique among each other, Steve took a moment to observe (Y/n). it was the first time he had actually sat together with her, despite the setting being in a group, but it was the first time he had an opportunity to interact with her; to really see her.

She was completely different than when Steve had first encountered her.

There was no more hostility and danger that stayed attached to her like a weapon in the earlier days of her recovery. No emptiness, no calculating looks, no cold precision that exuded from the soldier. Instead, Steve began to see a woman that was starting to become free from the weight of HYDRA one dry critique at a time.

She was sitting stiffly, her hands in her lap and shoulders small as if she wasn't sure if she was allowed to take up the space that she was in, but Steve watched as (Y/n)'s eyes darted around. She was listening, observing, learning, and living within the moment.

It made Steve feel happy despite the images that were still haunting his mind. Instead, a new image began to form: a woman who was slowly reclaiming her humanity; learning to be more than what HYDRA, Rollins, and Holloway had made her.

Steve was proud. He was proud of (Y/n), but he was also proud of the group attempting to give (Y/n) a sense of what human normalcy was. Steve glanced away from (Y/n) when Bucky nudged him softly, his face looking forlorn as he murmured.

"I also found some things out too...about Rebecca."

Steve was surprised, not having expected Bucky to mention his little sister, and he asked.

"What did you find out?"

Bucky's lips turned up slightly into a sad smile, glancing at Steve.

"I found out she had kids...a son and a daughter...and she named her son after me."

Steve was floored, the surprise evident on his face, and he asked.

"How did you find this out?"

Bucky swallowed thickly and explained quietly.

"I found (Y/n)'s Aunt Mavis...the woman that took (Y/n) to Shelbyville for the summers. Mavis and Rebecca knew each other, and then (Y/n) met Rebecca through Mavis. Mavis told me everything."

Steve was quiet, his eyes staring into Bucky's as the weight of his words settled over the man, and Steve finally asked him.

"Have you met them?"

Bucky let out a humorless chuckle.

"How could I? I doubt they would even know or remember anything about me. It'd be for the best to let them keep living their lives."

"Buck, you don't have to shut them out like that. I'm sure they'd love to get to know their uncle."

Bucky shook his head, his lips pressing into a line as he became apprehensive.

"I doubt they would want to know their uncle that used to be an assassin and killed hundreds of people."

Steve gave Bucky a firm look, but before Steve could reply, Tony exclaimed as he gestured to the show with a teasing tone to his voice.

"Hey, Cap, that guy kind of looks like you."

Steve spun his head around, the sudden callout immediately stealing his attention, and his eyes went onto the screen. There was a new character on the screen that he hadn't seen yet, black hair and blue eyes and a long trench coat on his shoulders. Steve frowned heavily in confusion, but before he could ask for elaboration, Tony stated.

"Emotionally constipated, confused, horrible sense of fashion. They've got you down to a T."

Steve looked taken back, an offended look coming upon his face.

"Horrible sense of fashion? I dress nicely."

Before Tony could retort, Sam pointed out with a playful but accusatory look on his face.

"I thought you hated the damn show. Why the hell are you still watching?"

Tony waved Sam off, shaking his head.

"It's helping me to debate on if I need to buy the studio to give them a few pointers and help them with that horrible writing."

Natasha chuckled, and (Y/n) suddenly glanced over at her, murmuring.

"Can we have another bath today?"

Natasha immediately nodded, giving (Y/n) a soft expression.

"After the day I've had? I think a bath and some wine sounds fantastic."

At the subtle mention of the court trial, (Y/n) became quiet, everyone within the room immediately noticing. (Y/n) looked down at her lap, fiddling with the hem of her shirt, and she murmured soft enough that it was almost a whisper.

"Thank you...for...fighting for me."

Her eyes flicked back up to the screen, her shyness slowly melting away again as (Y/n) began to watch the show again. Natasha was gently smiling at the woman, simply observing her, and Steve looked surprised by the shy thanks.

Tony's expression became observant as he watched the woman with a gentle look within his eyes. Bucky and Sam shared a look, but it was Tony who broke the silence and made (Y/n) look at him.

"We're the Avengers, kid, it's kind of what we do."

(Y/n) didn't say anything at first, only staring Tony down with a quiet look of content before she nodded once, and her eyes went back to the screen. Bucky glanced at Tony, who looked right back at him, and for once, Bucky felt a new sense of understanding build between them both.

Suddenly, Steve's phone rang, and Bucky immediately knew exactly who it was without having to look at the caller ID or inquire. Steve simply glanced at Bucky with a quiet nod before he stood, and Natasha sighed, stretching her legs.

"Alright, that's enough horrible writing for one night. Let's go take that bath, (Y/n). Maybe we can have Wanda join. I think she's gonna need a girl's night just as bad as we do."

(Y/n) nodded, slowly standing up, and her feet were silent as she padded across the room after Natasha. Steve left to a quieter part of the lab, and Sam, Bucky, and Tony were left to sit together.

The silence was comfortable but heavy, the show slowly being forgotten, and Shuri stepped forward finally, the men turning to look at her as she stood before them. Shuri ran a hand through her braids, sighing slightly.

"I have done some research into the locations that Rollins gave us during Mr. Rogers' interrogation. It seems the task of rooting out HYDRA will be much more difficult than we anticipated. Most of these locations are very high-profile places with many high-profile individuals. Taking these people down will only incite more legal ramifications...and will be a very long and difficult process."

Sam asked, crossing his arms as he sat back in his seat, immediately slipping back into business as he looked at the woman.

"How bad are we talking?"

Shuri sat down where (Y/n) and Natasha had been sitting, her frown deep as she spoke.

"We are no longer talking about abandoned HYDRA facilities or labs. We're talking about people in power and with intricate reach: CEO's, Senators, World Leaders, the list goes on. If we are not careful and do not strategize this to the very letter, we risk starting a war that we cannot control."

Bucky frowned, clenching his jaw slightly before he glanced down at the floor, and Tony sighed before asking Shuri.

"Alright, then what do you want to do?"

Shuri clasped her hands together, pursing her lips.

"With something so complex as this, I believe the best course of action is to enlist the help of Nicholas Fury. With Fury's extensive knowledge, background, and connections, we might be able to move much more quietly within the shadows."

Shuri paused before looking at the men within the room.

"Instead of HYDRA hiding among us...we must hide among them."

-

STORY NOTES: The scene opens to the Avengers returning to Wakanda after the court case, sullen despite the triumph over the case. When they go to Shuri's lab to discuss their next steps in taking down the rest of HYDRA, they are surprised to see Sam, Bucky and (Y/n) watching Supernatural. Sam and Bucky are bickering with each other about the action elements in the show, and (Y/n) begins to critique the show as well. At the revelation of (Y/n)'s relaxation and settling, Natasha points out the observation before engaging with (Y/n) about the show.

Steve sits to begin watching the show with them as well, and Tony makes his appearance to jab about the show. On the other side of the lab, Shuri quietly begins to research into the locations that Rollins had provided during his interrogation. Steve inquires about the show with Bucky, and Bucky explains that it was Sam's idea to put on and observes that (Y/n) seemed to be enjoying it despite her dry critiques. Bucky then tells Steve about his phone call with Dr. Raynor, and Steve says that Bucky must not be very happy about it. Bucky disagrees, telling Steve that (Y/n) will have a harder time adapting to society without professional help. Steve offers his input, stating that it would be good for her in the long run.

The moment is broken by (Y/n) critiquing the show again, and Tony agrees, making a joke about the show. The rest of the group chimes in and begins to bicker playfully with each other, Steve takes the time to observe (Y/n). He recalls how hostile (Y/n) had been during his first interaction with her when she was still under HYDRA's control and how different she is compared to now. He mulls over her a little bit more before thinking of how proud he is of the woman for trying, and his thoughts are broken by Bucky revealing the bombshell about his sister.

Bucky then goes on to tell Steve how he found the information out, telling Steve about (Y/n)'s Aunt Mavis, and Steve asks if Bucky has gone to meet his niece and nephew. Bucky tells him no, and remarks that he doubted they would want to meet him due to his past as the Winter Soldier. Before Steve can retort, Tony makes a jab about Steve being like Castiel, and the group begins to bicker again. The bickering is stopped by (Y/n) asking Natasha if they could take a bath later, and Natasha agrees. When Natasha makes a subtle comment about the court trial, (Y/n) becomes quiet before thanking the group for fighting for her. Tony responds by simple stating that they were the Avengers, and 'that's what we do'. At the sound of Steve's phone ringing, Natasha takes that as a cue to exit with (Y/n), and Sam, Bucky, and Tony are left alone together.

Shuri then approaches the three men, informing them of her research into the HYDRA facilities, and reveals that the locations and research indicate that the circumstances have become more complex due to the remainder of HYDRA being high-profile individuals. When Tony asks Shuri what her desire to do is, Shuri suggests calling Nicholas Fury for help due to his connections, background, and extensive knowledge of espionage. She suggests to turn the tides: Instead of HYDRA hiding within them, they were going to hide within HYDRA. End scene.

TRANSLATIONS:

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A Deep Dive into Pharmaceutical Formulation and Manufacturing

Pharmaceutical formulation and manufacturing are at the heart of bringing new drugs to market. These processes transform a scientific concept into a tangible product that can improve lives. This article explores the crucial stages of drug development, the challenges faced by manufacturers, and the innovations shaping the future of the industry. Whether you're a professional in the field or simply curious about how medicines are made, this deep dive will provide valuable insights.

The Critical Role of Pharmaceutical Formulation

Pharmaceutical formulation is the process of designing and developing a drug product that delivers the active pharmaceutical ingredient (API) effectively to the patient. This stage is vital because it determines the drug's stability, efficacy, and safety. Formulation scientists must consider factors like the drug's solubility, absorption rate, and shelf life. The goal is to create a product that is not only effective but also easy for patients to use. This might involve selecting the appropriate dosage form—such as tablets, capsules, or injectables—and ensuring that the drug remains stable under various conditions. As the first step in bringing a drug to market, formulation is where science meets practicality, laying the groundwork for successful treatment.

Key Stages in Drug Development

Pre-formulation Studies

Before a drug can be formulated, it must undergo pre-formulation studies. These studies analyze the physical and chemical properties of the active pharmaceutical ingredient (API). Key parameters such as solubility, stability, and bioavailability are assessed to determine how the drug will behave in the body. Understanding these characteristics is crucial because they influence the choice of formulation strategies. For example, a drug with poor solubility might require a different approach than one that is highly soluble. Pre-formulation studies provide the data needed to make informed decisions during the formulation process, ensuring that the final product will be both safe and effective.

Formulation Development

Once pre-formulation studies are complete, the focus shifts to formulation development. This stage involves creating a prototype of the drug product, which includes selecting excipients (inactive ingredients) that will work with the API to create a stable and effective product. The formulation must be optimized to achieve the desired therapeutic effect while minimizing side effects. This often requires multiple iterations and testing to find the right balance. The goal is to develop a product that not only meets regulatory standards but also addresses the needs of patients and healthcare providers.

Scale-Up and Manufacturing

After the formulation has been developed, the next step is to scale up production. This process involves translating the small-scale laboratory formulation into a manufacturing process that can produce large quantities of the drug consistently. Scale-up is a critical phase because it introduces new challenges, such as ensuring uniformity and quality across batches. Manufacturing must be carefully controlled to maintain the drug's integrity and efficacy. This stage also includes validation processes to demonstrate that the manufacturing methods are reliable and repeatable, which is essential for regulatory approval.

Challenges in Pharmaceutical Manufacturing

Regulatory Compliance

One of the most significant challenges in pharmaceutical manufacturing is navigating the complex regulatory landscape. Different countries have different regulations, and manufacturers must ensure that their products meet all applicable standards. This includes compliance with Good Manufacturing Practices (GMP), which set the guidelines for producing high-quality products consistently. Regulatory compliance is not just about meeting legal requirements; it's about ensuring that patients receive safe and effective medications. This process involves rigorous documentation, regular inspections, and staying up to date with changes in regulations.

Quality Assurance and Control

Quality assurance (QA) and quality control (QC) are critical components of pharmaceutical manufacturing. QA focuses on the overall process, ensuring that every step of production is performed according to established procedures. QC, on the other hand, involves testing the final product to confirm that it meets predefined standards. Both QA and QC are essential for maintaining the integrity of the drug product. Any deviation from quality standards can lead to product recalls, regulatory actions, or, worse, harm to patients. Manufacturers must implement robust QA and QC processes to minimize risks and ensure that every batch of medication is of the highest quality.

Innovations Driving the Future of Pharmaceutical Formulation

The pharmaceutical industry is continually evolving, driven by new technologies and scientific discoveries. Innovations such as personalized medicine, advanced drug delivery systems, and the use of artificial intelligence (AI) in formulation design are transforming how drugs are developed and manufactured. Personalized medicine, for instance, tailors treatments to individual patients based on their genetic profile, leading to more effective therapies with fewer side effects. Advanced drug delivery systems are improving the way drugs are administered, making them more targeted and reducing the potential for adverse reactions. AI is also playing a role in optimizing formulations, predicting how different ingredients will interact, and accelerating the development process. These innovations are not only improving patient outcomes but also making drug development more efficient and cost-effective.

Conclusion

The journey from concept to cure in pharmaceutical formulation and manufacturing is complex and challenging, but it is also filled with opportunities for innovation. By understanding the critical stages of drug development, the challenges faced by manufacturers, and the technological advancements shaping the future, we can appreciate the immense effort that goes into bringing new medications to market. As the industry continues to evolve, the focus will remain on improving patient care and delivering safe, effective, and accessible treatments worldwide.

[colinwilson11]

The global ophthalmic drugs has seen steady growth in recent years due to rising prevalence of eye diseases and an aging population. Key drivers behind this trend include growing incidence of chronic diseases like diabetes that can damage vision over time. Advancements in diagnosis and treatment have also improved health outcomes and boosted demand for specialized medications. However, drug development and manufacturing requires significant financial investments and dedicated facilities to adhere to stringent cGMP and regulatory compliance standards for products intended for ocular delivery. This is where contract service providers play a vital role in supporting drug companies through their expertise and scalable manufacturing capacity.