#MicroWellness

[themargauxs]

From the gym to the doctor’s office, wearable devices are building bridges between lifestyle and medicine

[der-saisonkoch]

Nasallo in Brauner Butter an neuer Kartoffel und Gemüse

Wir tauen den Nasallo in der Microwelle an. Dann schneiden wir ihn in Scheiben und legen den Fisch in einen Glasbehälter hitzefest. Gewürzt wird mit Salz, Pfeffer, mildem Weinessig. Wir geben ein paar Tropfen Öl auf den Fisch und schieben den 10-15 Minuten in den Grill – Oberhitze. Nicht zu nah an die Grillstäbe. Neue Kartoffel waschen wir gründlich und schneiden sie in Viertel oder Sechsten…

[cotaus-biomedical]

Cotaus Detachable White Frame Black Well ELISA Plate

Cotaus manufactures white frame black wells ELISA plates are 300 μL 96 microwell plates, round wells with flat bottom, made from polystyrene. These detachable ELISA strip plates are composed of 12 removable strips of 8 tubes, marked with letters (A-H) and numbers (1-12) in the vertical and horizontal directions, convenient for distinguishing, comparing and recording experiments.

◉ Produced and packed in a class 100,000 cleanroom ◉ Free of DNase, RNase, human DNA, and non-Pyrogenic ◉ Available in non-sterile, sterile packaging ◉ Pure white frame, perfectly fits the black 8-strip tubes, easy to disassemble ◉ The size of Cotaus® ELISA plates conforms to the SBS/ ANSI standards ◉ Uniform thickness and well size, good batch stability ◉ Uniform binding capacity, suitable for adsorption of hydrophobic molecules such as antigens, antibodies or proteins ◉ Black wells minimum light scattering and cross-talk during fluorescence assay, the white frame enhances light reflection and improves the signal intensity of luminescence detection ◉ Without lids, lids supplied separately ◉ Compatible with most ELISA readers, microplate readers, and easily integrated with high-throughput automated systems, supports custom

[hitechtraders]

Simplify Your Lab Work with a 96-Well Plate Mixer  

When it comes to modern lab equipment, efficiency is king. Enter the 96 well plate mixer, a must-have tool for scientists, technicians, and researchers aiming to streamline their workflows. Whether you're working in a hospital lab, biochemical research facility, or even a factory lab, this compact device is designed to mix solutions quickly and uniformly, eliminating the risk of contamination while ensuring precision every time.

What Is a 96-Well Plate?

Let’s start with the basics. A 96-well plate—also known as a microplate, microwell, or multiwell plate—is a flat plate with a grid of small wells that act as tiny test tubes. These plates are most commonly produced in a 2:3 rectangular format, featuring 96 wells.

While other formats like 384 or even 1536 wells are available, the 96-well format remains the go-to for most labs, owing to its versatility. These plates are compatible with both manual and automated laboratory equipment, making them the perfect middle ground for many applications.

Whether it's for enzyme-linked immunosorbent assays (ELISA), bacterial culture growth, nucleic acid purification, or high-throughput screening, the 96 well plate mixer ensures that each well is mixed efficiently, providing accurate and repeatable results.

Why the 96-Well Plate Mixer Is Essential

The 96 well plate mixer stands out for its simplicity and power, combining speed adjustment, timing, and oscillation modes that can handle everything from standard test tubes to microplates. Unlike manual shaking methods, this device minimizes human error while enhancing precision, allowing experiments to run smoothly without interruptions.

Its robust features cater to both novice and experienced lab personnel. For example, the clear LED display ensures easy readability, letting users set the time and speed with ease. Additionally, the high-speed DC brushless motor guarantees quiet operation, energy efficiency, and longevity—a solid investment for any lab.

Top Features of the 96-Well Plate Mixer

Let’s break down some of the standout features:

Precise Control: A bright LED display ensures that speed and timing settings are accurate and easy to manage. This is crucial for consistency across experiments.

Stable and Quiet: The high-speed DC brushless motor offers stable, noise-free operation, which is a game-changer (without using the word 'game-changer!'). You won’t be distracted by loud buzzing, and the motor’s long lifespan ensures it's maintenance-free.

Non-Slip Design: Thanks to its non-slip feet and low center of gravity, even high-speed operation doesn’t cause the machine to wander around the lab bench—no more worrying about runaway mixers.

Flexibility: The mixer’s various fixtures allow it to handle a range of sample types, from test tubes to ELISA plates, making it adaptable to whatever experiment you're conducting. Fixtures are automatically identified, with the speed limited accordingly to ensure safe operation.

Temperature Versatility: This mixer is built to handle a range of temperatures, working efficiently from 4°C to 65°C. Whether your lab runs cold or needs heat, this mixer can keep up.

Dynamic Acceleration: With an acceleration time of under 3 seconds, it gets right to business—no waiting around for the machine to catch up with your workflow.

Application and Benefits

The 96 well plate mixer doesn’t just offer efficiency—it provides peace of mind. Its robust design means you don’t have to babysit your experiments. Set the timer, adjust the speed, and let the mixer do the hard work. The quick acceleration, with Z-speed of less than three seconds, ensures that your solutions are ready in no time, without compromising the integrity of your samples.

For ELISA tests, where the uniform distribution of reagents is critical, the 96 well plate mixer is indispensable. The combination of rapid mixing and controlled oscillation guarantees that every well receives equal treatment, allowing for more reliable results.

Looking to Save on Lab Equipment?

Why pay top dollar for brand-new equipment when you can get reliable used lab gear at a fraction of the price? HiTechTrader offers a wide range of used laboratory equipment, including the 96 well plate mixer , at budget-friendly rates. A better lab experience starts with the right tools, and the 96 well plate mixer could be the upgrade your research needs.

For more information about Gas Chromatography Machine and Analytical Chemistry Equipment please visit:- Hi Tech Trader

[9plab]

A&o morgenroutiene (bezzeug gibts in die hand zur zimmernfckarte)*

Usb stecker am better war kaput, telefon ausgeschaltet ubernacht

Aufgeladen waered anziehen & zahneputzen

Fuer den kaffee nachgeschaut ob es ihrgend eine mglk gibt an heisses wasser zu kommen, eine microwelle endeckt

Wieder hoggelaufen in zimmer 210,kaffebecher ausgespuehlt wasser,gehohlt

Nach unten in die fruehstuckskantiene, becher in microwelle: in eine eckeck gestellt

Zur rezeption gelaufen gefragt ob sie eine schere haben, um die pfundkaffetuete aufzuschneiden

Ging!

Alee teile zusammen:)

*Den rezeptionisten gefragt ob der wirklich allein ist, das ich schon gesternn bei seiner kolegin gestaunt haette

(Bettzeug wird auch selbstandig (also vom kunden abgezogen)

Hochbetten knarzen, metallgestell mit mdf holzverkleidung,

Irgendjemand - der mich auch gestern sehr distanzlos ansprach machte morgens etwa um 6 das licht an, um sich anzuzihen, und sein bett aus

Dann weiteres uberlegt (telefon ist fast leer, gegessen hab ich noch nichts und ich muss auf klo & ich muss den kram noch verpacken,dafuer brauche ich eine schere

In “spaeti nach schere gefragt(heute das 2 malWTF wusste garnicht von der lebensnahen bedeutung dieses werkzeugs,

waren verpackt

Dann mit regionalban in der hoffnunfg ich lann auf klo und tlefom laden,

Beim flughafen gelandet(haha)

Es ist so absurd gleich 10 und ich binbimmer noch am flughafen, und mein telefon hat 4% - gefrühstückt hab ich persoenlich auch noch nicht

Also zu ikea? Pommes und Lightning usbc oder halt A kabel kaufen

Dann endlich weiter thinkpad hohlen

Paket abwerfen wenn telefon nicht ausgegangen,….

Ikea auf abendessen verschoben

WICHTIG auf dem berg

- powerbank eben da usb-c<>lightening?

-microfasertuch um x201 sauber,…..

Im buro? AUSDRUCK der REmail ombudsstelle

Wegkstatt? Loch duerch pantone =kugeschreiberleine,….

If SCHERE (wtf III) band iphone in huelle,….

Anwaelte abtel/ eben checken ob psyt(

Laden usb-A etwas weniger als 1%per min

[aktionfsa-blog-blog]

Saugt er nur oder schaut er auch?

Wir umgeben uns mit Wanzen

Nach dem Fernseher, dem Kühlschrank, der Microwelle, dem Smartphone wird nun auch der Staubsauger "digital" und bekommt Augen, Ohren und ein kleines Gedächtnis. Damit sind wir so weit "fortgeschritten", dass, wie der Stern berichtet, auch Serg Bell, der Gründer von Acronis und damit ein Urgestein der Cybersicherheit gegenüber "The Register" verraten hat, dass er Angst vor seinem eigenen Saugroboter hat.

Freiheit statt Angst !

Schon vor 13 Jahren hat unser damaliger Vereinsvorsitzender auf der Freiheit statt Angst Demo am Alexanderplatz gesagt: "Wir müssen selbst entscheiden, in welcher Welt wir zukünftig leben wollen ..." Es wurde immer mehr eine Welt der großen (Internet-) Konzerne, die bestimmen wollen, was wir zu kaufen und was wir zu denken haben. Damit muss Schluss sein!

Serg Bell jedenfalls weiß, dass alle diese Geräte mit ausgeklügelter Navigationstechnik, Mikrofonen, Kamera und Internetverbindung ein erhebliches Sicherheits- und Datenschutzrisiko darstellen können. Sie müssen es nicht in jedem Fall tun, aber wir werden es ihnen nie von außen ansehen, ob sie mit uns oder gegen uns arbeiten.

Normalerweise werden Menschen vorsichtiger im Umgang mit Dingen, die sie für wertvoll halten. Beim Umgang mit ihren persönlichen Daten und der Privatsphäre war das in den letzten 20 Jahren genau umgekehrt: Je mehr Daten wir erzeugen, desto freigiebiger werden wir mit diesen - sehr zum Nutzen der Big5, der GAFAM, der großen Internetkonzerne Google, Amazon, Facebook, Apple und Microsoft (s. Positive Anreize zur Selbstaufgabe ).

Gerade die Undurchschaubarkeit ist nach Ansicht von Serg Bell ein großes Problem, denn die Vielzahl solcher Geräte und unsere selbstgewählte Abhängigkeit von diesen macht es schwierig sich von ihnen zu lösen. Was funktioniert noch, wenn wir uns von ihnen in gefährlichen Situationen lösen müssen. Und wer verfügt dann über die jahrelang gesammelten Daten und kann sie auch dann noch gegen uns verwenden.

Die einzige Lösung, die er für uns derzeit parat hat, lautet entweder auf solche Geräte zu verzichten oder ihnen grundsätzlich den Zugang zum Internet zu verwehren, auch wenn das ihre Bedienbarkeit erschwert und ihre Funktionalität verringert.

Mehr dazu bei https://www.stern.de/digital/online/it-experte-in-sorge-vor-eigenem-saugroboter---und-hat-eine-warnung-32781992.html

Kategorie[21]: Unsere Themen in der Presse Short-Link dieser Seite: a-fsa.de/d/3qh Link zu dieser Seite: https://www.aktion-freiheitstattangst.org/de/articles/8179-20221016-saugt-er-nur-oder-schaut-er-auch.htm

[gbaishamerry39562-blog]

A new treatment to hair follicle germs for hair regeneration

A special research, from Yokohama National University, have developed a brand-new therapy to hair follicle germs for hair regeneration. According to the researchers, it is a method for the mass prep work of mobile aggregates, approximately 5000 HFGs all at once. Scientists carried out an animal research study by transferring the ready HFGs to generate hair roots and hairs on the computer mouse body. the searchings for highly suggest a function for boosting human hair regenerative treatment as a novel possible therapy for hair loss such as androgenic alopecia. The research have actually been released in the Journal of Biomaterials. Although loss of hair is not deadly, it troubles a substantial number of people all over the world, specifically in maturing cultures. Hair regenerative medication has become a new therapy to combat the issue. The therapy involves regrowing hair follicles, the small body organs that expand as well as sustain hair. Among the more difficult obstacles to hair regenerative medication has been the preparation of hair follicle germs, the reproductive source of hair roots, on a large scale. The paper, published in the journal Biomaterials, reports the effective preparation of up to 5000 HFGs all at once, as well as records brand-new hair development from the HFGs after transplantation into computer mice. " The key for the automation of HFGs was a selection of substrate products for society vessel," says the equivalent writer Junji Fukuda, Teacher, Yokohama National University. "We made use of oxygen-permeable dimethylpolysiloxane (PDMS) at the end of society vessel, and it functioned extremely well." The research group further evaluated the usefulness of this technique by moving the ready HFGs from a fabricated around 300-microwell array, gene database called "HFG chip," to produce hair roots and also hairs on the mouse body. If you have any sort of questions relating to where and ways to use microbiome project, you could contact us at our internet site. The group validated black hair generation at both the back and also scalp hair transplant sites. The regenerated hair displayed the normal hair cycle of murine hair. Fukuda claimed this straightforward technique is very robust and also promising. They wished that this method will certainly boost human hair regenerative therapy to deal with loss of hair such as androgenic alopecia. In fact, They have preliminary information that suggests human HFG development utilizing human keratinocytes and also dermal papilla cells. According to the scientists. For relevant recommendations, please usage CNGBdb Literature database.

[cotaus-biomedical]

ELISA plates, white frame with clear wells, detachable, 96 well microplate

Cotaus manufactures white frame clear wells ELISA plates are 300 μL 96 microwell plates, round well with flat bottom, made from polystyrene, and have been specifically surface-treated, available in universal, medium binding, high binding, and ultra-high binding ELISA plates. High-binding ELISA Plates have a binding capacity greater than 400 IgG/cm², enhancing protein adsorption for reliable and sensitive immunoassays.

◉ Produced and packed in a class 100,000 cleanroom ◉ Free of DNase, RNase, human DNA, and non-Pyrogenic ◉ Available in non-sterile, sterile packaging ◉ Detachable ELISA strip plates, composed of 12 breakable strips of 8 wells ◉ Pure white frame, perfectly fits the clear 8-strip tubes, easy to disassemble ◉ The size of Cotaus® ELISA plates conforms to the SBS/ ANSI standards, with a single-sided cut corner design on the upper left corner ◉ The ELISA plate is marked with letters (A-H) and numbers (1-12) in the vertical and horizontal directions, convenient for distinguishing, comparing and recording experiments ◉ Uniform thickness and well size, good batch stability ◉ High binding ELISA plates with a highly hydrophilic surface, superior adsorption capacity for IgG and other biomolecules ◉ White frame with clear wells high-binding ELISA plates, white frame enhances luminescence signals, while clear wells allow optimal optical readings and imaging, for both colorimetric and luminescent assays ◉ Without lids, low evaporation lids supplied separately ◉ Compatible with most ELISA readers, microplate readers (Absorbance Readers, Fluorescence Readers, Chemiluminescence Readers, Multimode Readers) and easily integrated with high-throughput automated systems, supports custom

[oeffentlicheversicherung]

@Der Marktplatz Vielleicht gibt es Interessenten für diese Microwelle mit Grill von @Lu-stig geht anders

[helvaticacare]

Cytokine Elisa Principle

The study of cytokines is useful in the investigation of inflammation. Cytokines are tiny proteins that convey information between cells and activate immunity in response to infection, inflammation, and other diseases. Accurate detection and measurement of cytokine concentrations are critical in characterising the nature of the infection and powerful in tracking illness progression.

Scientists are growing interested in enzyme labels and immunoassays that use enzyme-conjugated antibodies. The indirect sandwich enzyme-linked immunosorbent assay, or ELISA, is a low-cost analytical instrument used to analyse antibodies, antigens, proteins, and glycoproteins. There are numerous applications for cytokine analysis using ELISA, including those for neurological illnesses.

Helvetica Health Care (HHC) experts emphasise this significant medical application and how it aids in cytokine analysis.

What is elisa, and why is it used to detect cytokines?

The classic Sandwich ELISA test is a basic but extensively used instrument in medical laboratories throughout the world for detecting, measuring, and distinguishing a single cytokine from a mixture of biomolecules in any given sample.

ELISAs deliver specific, precise, and sensitive results, with the high-throughput rates required for cytokine protein analysis, whether the investigation is conducted in vitro or in vivo. In our whole blood assays, ELISA can typically assess cytokines produced in a culture medium, the concentration of ex vivo cytokine levels in plasma and serum, and triggered cytokine production.

Because it uses antibodies that are specific to a single cytokine or type, ELISA gives great precision. Sequential ELISA tests are used to evaluate several cytokines proteins from tiny samples.

How Does Elisas Function?

In principle, a basic sandwich ELISA analyses antigens between a layer of capture and detection antibodies. In other words, it is performed using an antibody-antigen-antibody sandwich. Below we explain the four steps of the simple indirect sandwich ELISA process:

1. Extracting analyte (specimen extracted for analysis) from a capture antibody sample.

2. Identifying biotin-labelled captured analyte with detection antibody (also specific for extracted analyte).

3. Detection amplification with streptavidin conjugated with an enzyme

4. Adding substrate and measuring the coloured signal via optical density (OD) using a microplate reader.

Scientists frequently use a microwell format (microwell plates) when conducting a traditional ELISA test.

Before we move on to a detailed explanation of the above steps, let us remind you that HHC provides a wide range of ELISA kits ready to use with break-apart wells. Our range of ELISA includes:

• The RETROTEK™ range is designed for the detection and quantitation of retroviral antigens from the retroviruses HIV-1, SIV and HTLV found in cell culture, serum, plasma or other biological fluids

• IMMUNOTEK™ kits can detect and quantify various immunoglobulins from many species, including Humans, Chicken, Cow, Goat, Rabbit, Rat and Mouse. We also provide HHV-6 IgG antibody and KSHV/HHV8 IgG antibody ELISA kits.

Describe the general elisa technique

Typically, the sample contains highly purified anti-cytokine antibodies, also known as capture antibodies, linked to an enzyme. Following plate washings, the immobilised antibodies are employed to capture the soluble cytokine proteins present in the applied samples. After washing the unbound material, biotin-conjugated anti-cytokine antibodies (detection antibodies) are utilised to identify the trapped cytokine proteins.

Following that is an enzyme-labeled avidin or streptavidin step. When a chromogenic substrate is introduced to the capture antibodies, it produces a signal that can be detected due to the substrate's colour change. The amount of coloured product produced by the bound, enzyme-linked detection reagents is simply quantified spectrophotometrically using an ELISA-plate reader at the appropriate optical density (OD). When the plate reader is connected to a computer, data storage and reanalysis are greatly facilitated.

A standard curve is introduced to a sandwich ELISA assay by serially diluting a standard cytokine protein solution with a preset concentration. Standard curves, also known as calibration curves, typically depict the standard cytokine protein concentration (usually ng or pg of cytokine/ml) vs the related mean OD value of duplicates. The concentrations of the potential cytokine-containing samples can be extrapolated from the standard curve.

The use of an ELISA computer application makes this operation easier. In general, completing a dilution series on the unknown samples helps to guarantee that the OD falls within the standard curve's linear range. The ELISA reagents used may have an impact on the curve fit analyses that researchers apply on their data, including either linear-log, log-log, or four-parameter transformations. 

Just like there are different types of cytokines, there exist other ELISAs procedures too:

· The Checkerboard method for optimising antibody concentrations

· The Dose-Response method for optimal blocking and dilution buffer for the sample matrix

· The Standard ELISA method for determining an analyte’s concentration in a given sample

· The Sequential ELISA

· The Multiplex ELISA

This fundamental approach can be improved by using sequential ELISA to test multiple analytes from a single sample aliquot. In this situation, a single sample is removed from one ELISA plate and then incubated in a different plate because the first plate would only catch the cytokine recognised by the antibody used for cytokine detection.

Multiplex ELISA is an excellent alternate method to the sequential ELISA wherein numerous capture antibodies are printed into a single microplate well, each with a different set of specificities.

Contact us at our Geneva office to inquire about the availability of our ELISA kits.

[rnomics]

Well-TEMP-seq – a microwell-based strategy for massively parallel profiling of single-cell temporal #RNA dynamics

The development of single-cell #RNA sequencing (scRNA-seq) technology has enabled researchers to study gene expression at the single-cell level, providing insights into cellular heterogeneity and dynamic... https://www.rna-seqblog.com/well-temp-seq-as-a-microwell-based-strategy-for-massively-parallel-profiling-of-single-cell-temporal-rna-dynamics/?utm_source=dlvr.it&utm_medium=tumblr

[moleculardepot]

Alkaline Phosphatase Blue Microwell Substrate

Alkaline Phosphatase Blue Microwell Substrate Catalog number: B2012715 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 100 mL Eq. Molecular Weight or Concentration: N/A Supplied as: Solution Applications: molecular tool for various biochemical applications Storage: 2-8°C Keywords: 5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays Grade:…