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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/abbkine-meets-you-at-17th-international-immunology-congress-iuis-2019/
Abbkine Meets You at 17th International Immunology Congress (IUIS 2019)
Abbkine invites you to visit IUIS 2019, Beijing, China.
Abbkine featured products and services will be displayed at the exhibition. There always be one type can flash into your eyes. Participate in interaction, more surprises are waiting for you!
Date: October 19 – 23, 2019
Booth：#D22，level 4
Address: China National Convention Center, Beijing, China
About: The 17th International Congress of Immunology (IUIS 2019) will be held from October 19 – 23, 2019 at the China National Convention Center, Beijing, China. IUIS 2019 promises to be an unforgettable event that will bring together delegates from all over the world. We anticipate over 5000 participants, including international leaders at the forefront of the discipline that will present the most recent advances in basic immunology and clinical translation.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/abbkine-meets-you-at-17th-international-immunology-congress-iuis-2019/
Abbkine Meets You at 17th International Immunology Congress (IUIS 2019)
Abbkine invites you to visit IUIS 2019, Beijing, China.
Abbkine featured products and services will be displayed at the exhibition. There always be one type can flash into your eyes. Participate in interaction, more surprises are waiting for you!
Date: October 19 – 23, 2019
Booth：#D22，level 4
Address: China National Convention Center, Beijing, China
About: The 17th International Congress of Immunology (IUIS 2019) will be held from October 19 – 23, 2019 at the China National Convention Center, Beijing, China. IUIS 2019 promises to be an unforgettable event that will bring together delegates from all over the world. We anticipate over 5000 participants, including international leaders at the forefront of the discipline that will present the most recent advances in basic immunology and clinical translation.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/in-4-years-abbkine-helped-more-than-1200-english-articles-to-be-published-impact-factors-exceed-4500-points/
In 4 years, Abbkine helped more than 1200 English articles to be published. Impact factors exceed 4500 points! ! !
Abbkine focuses on immunology and cytology, and is committed to innovating and developing various antibodies, proteins, analytical reagents and kits, with a view to becoming a key promoter in the fields of life science research and development, drug research and development, etc.
Some articles published using Abbkine products:
1. The negative effect of silica nanoparticles on adipogenic differentiation of human mesenchymal stem cells. Materials Science and Engineering. (IF: 27.24)
Using Abbkine Dylight 594, Goat Anti-Mouse IgG
2. Bph6 encodes an exocyst-localized protein and confers broad resistance to planthoppers in rice. NATURE GENETICS. (IF: 19.88)
Using Abbkine Anti-Plant Actin Mouse Monoclonal Antibody (3T3)
3. The deubiquitinating enzyme cylindromatosis mitigates nonalcoholic steatohepatitis. . (IF: 19.14)
Using Abbkine HRP, Goat Anti-Rabbit IgG
4. Research on the interaction of protein Tmub1 and securin after partial hepatectomy. Journal of the American Chemical Society. (IF: 14.75)
Using Abbkine IFKine™ Red Donkey Anti-Rabbit IgG
5. Verification of Differential Expression Genes after CacyBP/SIP Nuclear Translocation in Colon Carcinoma Cell Line. Journal of the American Chemical Society. (IF: 14.75)
Using Abbkine Anti-PCNA Mouse Monoclonal Antibody (1D7)
6. CD8+T cells induce cachexia during chronic viral infection. NATURE IMMUNOLOGY. (IF: 14.71)
Using Abbkine EliKine™ Free Triiodothyronine (fT3) ELISA Kit
7. SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1. Nucleic Acids Research. (IF: 11.14)
Using Abbkine Pan Methylated Lysine Monoclonal Antibody (Mix)
8. The lncRNA PVT1 regulates nasopharyngeal carcinoma cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1α. Cell Death & Differentiation. (IF: 8.086)
Using Abbkine KAT6A Polyclonal Antibody
9. DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B. Autophagy. (IF: 7.01)
Using Abbkine IPKine™ HRP, Goat Anti-Mouse IgG HCS
10. CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis. Autophagy. (IF: 7.01)
Using Abbkine IPKine™ HRP, Goat Anti-Rabbit IgG HCS
Some partners of Abbkine:
Harvard University, Georgia Institute of Technology, Oxford University, Duke University, Stanford University, Peking University, Tsinghua University, Zhejiang University, Chinese Academy of Sciences, Wuhan University, Fudan University.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/research-hotspots-of-cell-apoptosis-bcl-2-monoclonal-antibody/
Research Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody
【Promotion】Abbkine Selected Primary Antibody-Click for Promotion details.
Bcl-2 gene (B-cell lymphoma-2) is a member of Bcl-2 family, which is the earliest discovered apoptosis protein. It is an anti-apoptosis protein containing multiple BH domains. It is highly expressed in some cancer cells and selectively plays an anti-tumor role. Bcl-2 family proteins play an important role in the regulation of apoptosis by forming dimers with Bax and dimerizing themselves. When Bcl-2 protein is inhibited, its dimer with Bax decreases, leading to cell apoptosis. When Bcl-2 protein is overexpressed, heterodimers formed by Bcl-2 protein and Bax increase and apoptosis is inhibited. The balance between Bcl-2 and Bax proteins at the cell death signal checkpoint determines the survival or apoptosis of cells.
Fig: Bcl-2 and Cell apoptosis
We recommend Abbkine selected Bcl-2 antibody for WB, IHC-P and IF experiments in human, rats, mice and chickens.
Product name Cat# Application MW Bcl-2 Monoclonal Antibody ABM0010 WB（1：1000-2000），IF（1:50-200），IHC-P（1:200） 26KD
The result:
1) Hela cells; 2) MCF-7 cells; Using Abbkine Bcl-2 monoclonal antibody (ABM0010), WB 1:1000 dilution.
Human oviduct, Using Abbkine Bcl-2 monoclonal antibody (ABM0010) and IHC-P 1:100.
NIH/3T3 cells, Using Abbkine Bcl-2 monoclonal antibody (ABM0010), green, IF 1:100.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/beyond-brdu-new-generation-cell-proliferation-imaging-analysis-kit-edu-method/
Beyond BrdU, New Generation Cell Proliferation Imaging Analysis Kit (EdU Method)
Common methods of cell proliferation mainly include MTT method, WST-1 method and CCK-8 method, which are mainly based on the activity of cells, thus reflecting the overall proliferation effect, but unable to detect individual proliferating cells. Recognized as the most accurate method to detect cell proliferation is to directly detect DNA synthesis in cells. Previously, BrdU method was the most commonly used method. However, the method requires DNA denaturation (such as acid denaturation, thermal denaturation or DNase digestion) to expose BrdU, thus binding to BrdU antibody. The whole experiment has many influencing factors and poor stability.
Abbkine innovates and develops EdU method based on EdU incorporation and subsequent click reaction to solve this application difficulty:
Product name Cat# Fluorescent characteristic Cell Proliferation EdU Image Kit (Green Fluorescence) KTA2030 AbFluor 488 Ex/Em = 501/525 nm Cell Proliferation EdU Image Kit (Orange Fluorescence) KTA2031 AbFluor 545 Ex/Em = 546/565 nm
Principle：EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, is complete within 30 minutes.
Kit components Advantage • EdU (10mM)
• AbFluor 488 azide or AbFluor 545 azide
• 10×Reaction buffer
• Copper
• Reducing Agent • Not antibody based
• No DNA denaturation. Can maintain cell morphology and DNA integrity
• Patented AbFluor 488 and 545 azide have good light stability and quenching resistance
• Optimization for fluorescence microscope
Results:
KTA2030 Imaging: Hela Cells (Green) KTA2031 Imaging: Hela Cells (Red)
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/loading-control-tag-antibody-buy-two-get-one-for-free/
Loading Control & Tag Antibody-Buy two get one for free
Flash sale: From now to October 31st, 2019
Promotion product list: Classical loading control and tag antibodies and the conjugated versions of these antibodies, including HRP, Biotin, FITC, Cy3, Cy5 and AbFlour TM dyes.
Details of activities: Buy 2 get 1 for free (if a single order is full of 3 antibodies, the antibody with the lowest price will be exempted).
Why choose Abbkine loading control and tag antibodies?
Abbkine offers a wide range of loading control and tag antibodies, including the hot targets and unique species, covering the molecular weights from 15 kDa to 116 kDa.
Abbkine conjugated loading control and tag antibodies have diverse conjugates, including enzyme and featured fluorescent dyes, with the application of WB, IHC, IF and IP experiments.
Abbkine loading control and tag antibodies are verified through rigorous procedures, popular with customers for their good performance and high sensitivity.
Different sizes for clients to choose, small size and bulk size are available, both with high cost-effectiveness.
Part of promotion product list:
Classical loading control and tag antibodies (Citation of over 100 SCI Documents)
Cat# Product name Application size A01010 Anti-β-Actin Mouse Monoclonal Antibody (1C7) WB#IHC-P#IF 50/200/200x5ul A01020 Anti-GAPDH Mouse Monoclonal Antibody (2B5) WB#IHC-P#IF 50/200/200x5ul A01030 Anti-β-Tubulin Mouse Monoclonal Antibody (3G6) WB#IHC-P#IF 50/200/200x5ul A01050 Anti-Plant Actin Mouse Monoclonal Antibody (3T3) WB 50/200/200x5ul A02010 Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) WB#IF#IP 50/200/200x5ul A02050 Anti-His Tag Mouse Monoclonal Antibody (5C3) WB#IF#IP 50/200/200x5ul A02040 Anti-HA Tag Mouse Monoclonal Antibody (4F6) WB#IF#IP 50/200/200x5ul A02020 Anti-GFP Tag Mouse Monoclonal Antibody (3D3) WB#IP 50/200/200x5ul A02030 Anti-GST Tag Mouse Monoclonal Antibody (2A8) WB 50/200/200x5ul A02060 Anti-Myc Tag Mouse Monoclonal Antibody (2D5) WB#IF#IP 50/200/200x5ul A02080 Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) WB#IF 50/200/200x5ul
IP experiment partner-Agarose/Magnetic beads conjugated tag antibodies
Compared with the traditional immunoprecipitation (IP) method using Protein A/G, Abbkine's agarose/magnetic bead coupling labeled antibody eliminates the step of Protein A/G binding with antigen-antibody complex. After the conjugated antibody is directly combined with the target protein, the target protein is separated from the cell lysate by centrifugation or using a magnetic rack. It not only makes the experiment simple and fast, but also can avoid non-specific binding and reduce the background.
Cat# Product name Application size A02010AGB Anti-DDDDK Tag Mouse Mab (1B10), Agarose IP 100ul/400ul/2ml A02010MGB Anti-DDDDK Tag Mouse Mab (1B10), Magnetic Beads IP 100ul/400ul/2ml A02040AGB Anti-HA Tag Mouse Mab (4F6), Agarose IP 100ul/400ul/2ml A02040MGB Anti-HA Tag Mouse Mab (4F6), Magnetic Beads IP 100ul/400ul/2ml A02050AGB Anti-His Tag Mouse Mab (5C3), Agarose IP 100ul/400ul/2ml A02050MGB Anti-His Tag Mouse Mab (5C3), Magnetic Beads IP 100ul/400ul/2ml A02060AGB Anti-Myc Tag Mouse Mab (2D5), Agarose IP 100ul/400ul/2ml A02060MGB Anti-Myc Tag Mouse Mab (2D5), Magnetic Beads IP 100ul/400ul/2ml A02170AGB Anti-V5 Tag Mouse Mab (11D5), Agarose IP 100ul/400ul/2ml A02170MGB Anti-V5 Tag Mouse Mab (11D5), Magnetic Beads IP 100ul/400ul/2ml A02180AGB Anti-VSV-G-Tag Mouse Mab (14D2), Agarose IP 100ul/400ul/2ml A02180MGB Anti-VSV-G-Tag Mouse Mab (14D2), Magnetic Beads IP 100ul/400ul/2ml
For the full product promotion list, please contact Abbkine's local distributor or contact [email protected] directly. Abbkine has the final right to interpret this activity.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/loading-control-tag-antibody-buy-two-get-one-for-free/
Loading Control & Tag Antibody-Buy two get one for free
Flash sale: From now to October 31st, 2019
Promotion product list: Classical loading control and tag antibodies and the conjugated versions of these antibodies, including HRP, Biotin, FITC, Cy3, Cy5 and AbFlour TM dyes.
Details of activities: Buy 2 get 1 for free (if a single order is full of 3 antibodies, the antibody with the lowest price will be exempted).
Why choose Abbkine loading control and tag antibodies?
Abbkine offers a wide range of loading control and tag antibodies, including the hot targets and unique species, covering the molecular weights from 15 kDa to 116 kDa.
Abbkine conjugated loading control and tag antibodies have diverse conjugates, including enzyme and featured fluorescent dyes, with the application of WB, IHC, IF and IP experiments.
Abbkine loading control and tag antibodies are verified through rigorous procedures, popular with customers for their good performance and high sensitivity.
Different sizes for clients to choose, small size and bulk size are available, both with high cost-effectiveness.
Part of promotion product list:
Classical loading control and tag antibodies (Citation of over 100 SCI Documents)
Cat# Product name Application size A01010 Anti-β-Actin Mouse Monoclonal Antibody (1C7) WB#IHC-P#IF 50/200/200x5ul A01020 Anti-GAPDH Mouse Monoclonal Antibody (2B5) WB#IHC-P#IF 50/200/200x5ul A01030 Anti-β-Tubulin Mouse Monoclonal Antibody (3G6) WB#IHC-P#IF 50/200/200x5ul A01050 Anti-Plant Actin Mouse Monoclonal Antibody (3T3) WB 50/200/200x5ul A02010 Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) WB#IF#IP 50/200/200x5ul A02050 Anti-His Tag Mouse Monoclonal Antibody (5C3) WB#IF#IP 50/200/200x5ul A02040 Anti-HA Tag Mouse Monoclonal Antibody (4F6) WB#IF#IP 50/200/200x5ul A02020 Anti-GFP Tag Mouse Monoclonal Antibody (3D3) WB#IP 50/200/200x5ul A02030 Anti-GST Tag Mouse Monoclonal Antibody (2A8) WB 50/200/200x5ul A02060 Anti-Myc Tag Mouse Monoclonal Antibody (2D5) WB#IF#IP 50/200/200x5ul A02080 Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) WB#IF 50/200/200x5ul
IP experiment partner-Agarose/Magnetic beads conjugated tag antibodies
Compared with the traditional immunoprecipitation (IP) method using Protein A/G, Abbkine's agarose/magnetic bead coupling labeled antibody eliminates the step of Protein A/G binding with antigen-antibody complex. After the conjugated antibody is directly combined with the target protein, the target protein is separated from the cell lysate by centrifugation or using a magnetic rack. It not only makes the experiment simple and fast, but also can avoid non-specific binding and reduce the background.
Cat# Product name Application size A02010AGB Anti-DDDDK Tag Mouse Mab (1B10), Agarose IP 100ul/400ul/2ml A02010MGB Anti-DDDDK Tag Mouse Mab (1B10), Magnetic Beads IP 100ul/400ul/2ml A02040AGB Anti-HA Tag Mouse Mab (4F6), Agarose IP 100ul/400ul/2ml A02040MGB Anti-HA Tag Mouse Mab (4F6), Magnetic Beads IP 100ul/400ul/2ml A02050AGB Anti-His Tag Mouse Mab (5C3), Agarose IP 100ul/400ul/2ml A02050MGB Anti-His Tag Mouse Mab (5C3), Magnetic Beads IP 100ul/400ul/2ml A02060AGB Anti-Myc Tag Mouse Mab (2D5), Agarose IP 100ul/400ul/2ml A02060MGB Anti-Myc Tag Mouse Mab (2D5), Magnetic Beads IP 100ul/400ul/2ml A02170AGB Anti-V5 Tag Mouse Mab (11D5), Agarose IP 100ul/400ul/2ml A02170MGB Anti-V5 Tag Mouse Mab (11D5), Magnetic Beads IP 100ul/400ul/2ml A02180AGB Anti-VSV-G-Tag Mouse Mab (14D2), Agarose IP 100ul/400ul/2ml A02180MGB Anti-VSV-G-Tag Mouse Mab (14D2), Magnetic Beads IP 100ul/400ul/2ml
For the full product promotion list, please contact Abbkine's local distributor or contact [email protected] directly. Abbkine has the final right to interpret this activity.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/selection-strategy-of-apoptosis-products-late-apoptosis/
Selection Strategy of Apoptosis Products- Late apoptosis
Apoptosis is an orderly and programmed death followed by cells under the influence of physiological or pathological factors in order to maintain internal environment stability. Apoptosis occurs in cells. First, the cell volume shrinks and the connection disappears. Then, the density of cytoplasm increases, mitochondrial membrane potential disappears, permeability changes, cytochrome C is released to cytoplasm, nuclear substance is concentrated, nuclear membrane nucleolus is broken, DNA is degraded into fragments, and finally apoptotic bodies are formed and engulfed by macrophages.
Late apoptosis
In cell apoptosis, especially in the late stage of apoptosis, chromosomal DNA will be broken, resulting in a large number of sticky 3'-OH ends. Under the action of deoxynucleotide terminal transferase (TdT), derivatives formed by deoxyuridine triphosphate nucleotide (dUTP) and fluorescein can be labeled to the 3'- end of DNA, namely deoxynucleotide terminal transferase mediated nick end labeling (TUNEL). Normal or proliferating cells have few DNA breaks and can rarely be stained. Therefore, TUNEL is the most commonly used method to detect DNA fragmentation in late apoptosis.
Product recommendation
Product name Cat# TUNEL Apoptosis Detection Kit (Green Fluorescence) KTA2010 TUNEL Apoptosis Detection Kit (Orange Fluorescence) KTA2011
Product character
Abbkine's TUNEL apoptosis detection kit provides a complete set of reagent components and optimized experimental scheme required for the experiment. It is suitable for a variety of instruments such as fluorescence enzyme labeling instrument, fluorescence microscope, flow cytometer, etc. It can be used to detect adherent cells, suspended cells, paraffin-embedded tissue sections, frozen sections and other sample types.
Apoptosis cannot be stopped once it starts, it is a highly regulated process.
Apoptosis can be initiated by two initial pathways. The internal initial pathway is activated by non-receptor stimulation, such as DNA damage, endoplasmic reticulum stress, metabolic stress, mitochondrial outer membrane permeability changes, such as cytochrome C release. The external pathway starts by binding death receptor to ligand (such as FasL, tumor necrosis factor TNF). After that, the two approaches converge in caspase cascade reaction, and cell death is induced by activating caspase protease or protein degrading enzyme. Anti-apoptotic ligands, such as cytokines and growth factors, promote cell survival, proliferation and differentiation through different signaling molecules such as AKT and p90RSK and anti-apoptotic proteins such as Bcl-2 and Bcl-x. The withdrawal of these cytokines and growth factors leads to cell death.
Product recommendation
Product name Cat# Application Bax Mouse Monoclonal Antibody (6F11) ABM40273 IF, IHC-p, WB Bcl-2 Monoclonal Antibody ABM0010 IF, IHC-p, WB Bad Polyclonal Antibody ABP55880 ELISA, IF, IHC-p, WB p53 Monoclonal Antibody ABM0016 IF, IHC-p, WB FAS Polyclonal Antibody ABP51327 ELISA, IF, IHC-p, WB Caspase-3 Polyclonal Antibody ABP52935 ELISA, IF, IHC-p, WB Caspase-8 Monoclonal Antibody ABM0053 IF, IHC-p, WB Caspase 9 Monoclonal Antibody ABM0028 IF, IHC-p, IP, WB NFkB p65 Monoclonal Antibody ABM40111 IF, IHC-p, IP, WB
Product character
Abbkine has selected the antibody of apoptosis series. After multiple strict verifications, it is suitable for a variety of cell applications and meets the research needs of most customers. The excellent results are as follows:
IHC analysis of paraffin-embedded human uterine tissue was performed using Bad polyclonal antibody diluted at 1: 200.
IF analysis of rat lung tissue was performed using Caspase-3 polyclonal antibody diluted at 1: 200.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/selection-strategy-of-apoptosis-products-early-apoptosis/
Selection Strategy of Apoptosis Products- Early apoptosis
Apoptosis is a dynamic process, which involves a series of complex biochemical reactions, expression regulation of multiple genes, signal transduction, cascade reactions involving multiple enzymes and multiple signal pathways. The cell feels the corresponding apoptosis signal stimulation, and a series of control switches in the cell are turned on or off, and activation of various enzymes triggers a series of cascade reactions. Different external factors initiate apoptosis in different ways, resulting in different signal transduction.
Early apoptosis
Feature 1: eversion of lipid membrane inside
In normal cells, phosphatidylserine (PS) is only distributed on the inner side of the lipid bilayer of the cell membrane. In the early stage of cell apoptosis, PS turns from the inner side of the lipid membrane to the outer side. Annexin V is a calcium-dependent phospholipid binding protein with a molecular weight of 35-36kD, and has high affinity with PS. It binds to the cell membrane of early apoptotic cells through PS exposed on the outside of cells. Therefore Annexin V is a sensitive indicator to detect early apoptosis of cells. Usually, Annexin V is labeled with some fluorescent probes, which can simply and directly detect PS eversion, an important feature of apoptosis.
Product recommendation
Product name Cat# Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit KTA0001 Abbkine Annexin V-AbFluor™ 488 Apoptosis Detection Kit KTA0002 Abbkine Annexin V-AbFluor™ 555 Apoptosis Detection Kit KTA0003 Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit KTA0004
Product character
AbFlourTM series of characteristic fluorescent dyes (blue, green and red) patented by Abbkine label recombinant human Annexin V protein, and are matched with PI to introduce different fluorescence apoptosis detection kits, which are suitable for detection with fluorescence equipment such as flow cytometry and fluorescence microscope.
Feature 2: Mitochondrial membrane potential decreased
Normal mitochondrial membrane potential is the premise for maintaining mitochondrial oxidative phosphorylation and ATP production, and is necessary for maintaining mitochondrial function. The decline of mitochondrial membrane potential is a landmark event in the early stage of cell apoptosis. JC-1 is an ideal fluorescent probe widely used for detecting mitochondrial membrane potential. When mitochondrial membrane potential is high, JC-1 aggregates in the matrix of mitochondria to form polymers (J-aggregates). Red fluorescence can be generated (Ex/Em = 585/590). when the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria. at this time, JC-1 is a monomer, which can generate green fluorescence (Ex/Em = 510/527 nm).
Product recommendation
Product name Cat# Mitochondrial Membrane Potential Assay Kit (JC-1) KTA4001
Product character
Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1) uses JC-1 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential. This kit can very conveniently detect changes in mitochondrial membrane potential through changes in fluorescence color.
Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1)
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/selection-strategy-of-apoptosis-products-early-apoptosis/
Selection Strategy of Apoptosis Products- Early apoptosis
Apoptosis is a dynamic process, which involves a series of complex biochemical reactions, expression regulation of multiple genes, signal transduction, cascade reactions involving multiple enzymes and multiple signal pathways. The cell feels the corresponding apoptosis signal stimulation, and a series of control switches in the cell are turned on or off, and activation of various enzymes triggers a series of cascade reactions. Different external factors initiate apoptosis in different ways, resulting in different signal transduction.
Early apoptosis
Feature 1: eversion of lipid membrane inside
In normal cells, phosphatidylserine (PS) is only distributed on the inner side of the lipid bilayer of the cell membrane. In the early stage of cell apoptosis, PS turns from the inner side of the lipid membrane to the outer side. Annexin V is a calcium-dependent phospholipid binding protein with a molecular weight of 35-36kD, and has high affinity with PS. It binds to the cell membrane of early apoptotic cells through PS exposed on the outside of cells. Therefore Annexin V is a sensitive indicator to detect early apoptosis of cells. Usually, Annexin V is labeled with some fluorescent probes, which can simply and directly detect PS eversion, an important feature of apoptosis.
Product recommendation
Product name Cat# Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit KTA0001 Abbkine Annexin V-AbFluor™ 488 Apoptosis Detection Kit KTA0002 Abbkine Annexin V-AbFluor™ 555 Apoptosis Detection Kit KTA0003 Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit KTA0004
Product character
AbFlourTM series of characteristic fluorescent dyes (blue, green and red) patented by Abbkine label recombinant human Annexin V protein, and are matched with PI to introduce different fluorescence apoptosis detection kits, which are suitable for detection with fluorescence equipment such as flow cytometry and fluorescence microscope.
Feature 2: Mitochondrial membrane potential decreased
Normal mitochondrial membrane potential is the premise for maintaining mitochondrial oxidative phosphorylation and ATP production, and is necessary for maintaining mitochondrial function. The decline of mitochondrial membrane potential is a landmark event in the early stage of cell apoptosis. JC-1 is an ideal fluorescent probe widely used for detecting mitochondrial membrane potential. When mitochondrial membrane potential is high, JC-1 aggregates in the matrix of mitochondria to form polymers (J-aggregates). Red fluorescence can be generated (Ex/Em = 585/590). when the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria. at this time, JC-1 is a monomer, which can generate green fluorescence (Ex/Em = 510/527 nm).
Product recommendation
Product name Cat# Mitochondrial Membrane Potential Assay Kit (JC-1) KTA4001
Product character
Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1) uses JC-1 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential. This kit can very conveniently detect changes in mitochondrial membrane potential through changes in fluorescence color.
Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1)
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/abbkine-chekine-lactate-dehydrogenase-ldh-assay-kit/
Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method
Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.
Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.
Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive
Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110
【Principle】
provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.
【Samples】
serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.
【Kit components】
Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve
【Procedure】
Working Reagent Preparation
Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).
Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).
【Standard Curve】
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/abbkine-chekine-lactate-dehydrogenase-ldh-assay-kit/
Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method
Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.
Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.
Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive
Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110
【Principle】
provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.
【Samples】
serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.
【Kit components】
Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve
【Procedure】
Working Reagent Preparation
Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).
Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).
【Standard Curve】
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/abbkine-chekine-lactate-dehydrogenase-ldh-assay-kit/
Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method
Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.
Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.
Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive
Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110
【Principle】
provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.
【Samples】
serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.
【Kit components】
Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve
【Procedure】
Working Reagent Preparation
Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).
Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).
【Standard Curve】
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.
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Text
New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/abbkine-chekine-lactate-dehydrogenase-ldh-assay-kit/
Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method
Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.
Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.
Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive
Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110
【Principle】
provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.
【Samples】
serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.
【Kit components】
Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve
【Procedure】
Working Reagent Preparation
Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).
Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).
【Standard Curve】
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/chekine-xanthine-oxidase-assay-kit-a-great-tool-for-study-of-hyperuricemia/
CheKine™ Xanthine Oxidase Assay Kit, a great tool for study of hyperuricemia
Xanthine oxidase is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. These enzymes catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. These enzymes play an important role in the catabolism of purines in some species, including humans.
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. Most of the protein in the liver exists in a form with xanthine dehydrogenase activity, but it can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. In various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
Abbkine CheKine™ Xanthine Oxidase Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of the XO present in a variety of samples. In the assay, XO oxidizes xanthine to superoxide (O2 - ). Superoxide (O2 - ) reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically at OD 450 nm. Therefore, the Xanthine Oxidase present in the sample is proportional to the signal obtained.
Product Name CAT # Detection Range CheKine™ Xanthine Oxidase Assay Kit KTB1070 15.6 -500 mU/mL
【Kit components】
• Assay Buffer
• Sample Diluent
• WST-8
• Enhancer
• Xanthine Oxidase Standard (10 U/mL)
• Xanthine
【Procedure】
Prepare the Working Reagent.
Add standard and sample per well. Then add Working Reagent per well quickly. Tap plate to mix briefly and thoroughly. Immediately read optical density at 450nm (OD0).
Incubate for 30 min at room temperature (25°C) in the dark. Read optical density at 450nm again (OD30).
【Standard Curve】
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.
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New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/glucose-oxidase-activity-assay-kit/
New biosensor weapons, CheKine™ Glucose Oxidase Activity (GOD)Assay Kit
Glucose oxidase is a an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present. Glucose oxidase is widely used to measure glucose in body fluids, as well as residual glucose and oxygen from beverages, foods and other agricultural products. In addition, glucose oxidase is commonly used as biosensors to detect glucose.
Test the activity of glucose oxidase (GOD), recommend the Abbkine CheKine™ glucose oxidase (GOD) activity kit for you, details are as follows:
Product Name CAT # Detection Range CheKine™ Glucose Oxidase Activity (GOD)Assay Kit KTB1310 0.05 - 2 U/L
【Principle】
In this assay, GOD activity is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with chromogen in an acid condition, produce a product that can be measured at OD 580 nm. Therefore, the glucose oxidase activity present in the sample is proportional to the signal obtained.
【Sample】
Serum, Plasma, Cells, Tissues, Urine, Food, Medium, other body fluids.
【Kit components】
Assay Buffer (20x)
Glucose (0.2 M)
H2O2 Standard (0.88 M)
Chromogen
Glucose Oxidase (control)
【Simple procedure】
Prepare standards and sample, add Glucose to each tube, mix and incubate for 20 min at 37°C.
Transfer the mixture into a clear bottom 96-well plate, add Chromogen per well, mix and read optical density at 580nm(OD0). Incubate for 10 min at 37°C in the dark. Read optical density at 580nm again(OD10).
【Standard Curve】
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.
0 notes
Text
New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on https://www.abbkine.com/glucose-oxidase-activity-assay-kit/
New biosensor weapons, CheKine™ Glucose Oxidase Activity (GOD)Assay Kit
Glucose oxidase is a an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present. Glucose oxidase is widely used to measure glucose in body fluids, as well as residual glucose and oxygen from beverages, foods and other agricultural products. In addition, glucose oxidase is commonly used as biosensors to detect glucose.
Test the activity of glucose oxidase (GOD), recommend the Abbkine CheKine™ glucose oxidase (GOD) activity kit for you, details are as follows:
Product Name CAT # Detection Range CheKine™ Glucose Oxidase Activity (GOD)Assay Kit KTB1310 0.05 - 2 U/L
【Principle】
In this assay, GOD activity is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with chromogen in an acid condition, produce a product that can be measured at OD 580 nm. Therefore, the glucose oxidase activity present in the sample is proportional to the signal obtained.
【Sample】
Serum, Plasma, Cells, Tissues, Urine, Food, Medium, other body fluids.
【Kit components】
Assay Buffer (20x)
Glucose (0.2 M)
H2O2 Standard (0.88 M)
Chromogen
Glucose Oxidase (control)
【Simple procedure】
Prepare standards and sample, add Glucose to each tube, mix and incubate for 20 min at 37°C.
Transfer the mixture into a clear bottom 96-well plate, add Chromogen per well, mix and read optical density at 580nm(OD0). Incubate for 10 min at 37°C in the dark. Read optical density at 580nm again(OD10).
【Standard Curve】
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.
0 notes