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HPLC Pump Care and Troubleshooting

HPLC pumps require routine maintenance and care to keep them operating smoothly. The first step in proper pump maintenance is to check for errors. Error messages such as "index adjustment error" or "index limit error" indicate that something is wrong with the pump. The cause of the error can be the pump head or its drive assembly. If the pump's drive is out of order, it will need to be replaced.

Another way to make sure that your HPLC system is working properly is to check the pressure daily. Although this pressure will vary depending on the method used, it should not be excessively low or high. In addition, you should record the pressure readings in a logbook. Keeping these records will help you troubleshoot problems as they occur. Go to: http://www.chromtech.com/ to learn more about HPLC pumps.

Regularly replacing seals is another important maintenance step. Leaky seals can affect the performance of HPLC pumps. In addition, caustic mobile phases can damage the pump's seals. Proper maintenance will increase the longevity of the pump system. Also, check for blockages in the waste line and loop. Properly slopping the waste line and keeping it above the surface can prevent leaks.

Filters play an important role in maintaining the performance of HPLC pumps. A dirty filter can disrupt smooth and turbulent flow. Some inline filters are built into the pump, but others are separate from the pump. You should change the filter every three months or as required. You should also replace the PTFE frit regularly. Cleaning the filters is another important part of regular maintenance.

Most pump-related issues are caused by failure to prime the system. Always prime the pump before using it. If you do not prime the pump, the fluid flow will be either very low or extremely low, which will affect your chromatography results. If the solvent flow is low or erratic, it will result in an inaccurate gradient, or even no gradient at all. Also, if the pump is not primed properly, the system may not be capable of mixing solvents in the appropriate proportions. For more enlightening info about the topic, click to read.

Proper HPLC pump care and troubleshooting is vital to the efficient operation of HPLCs. If you notice any problems, contact a reliable maintenance company and they will help you troubleshoot and repair the HPLC pump. The cost of repairing HPLCs is high, and premature replacement of parts may be costly.

During the pump's operation, you should check its inlet filter periodically. If it is plugged, cavitation or other problems can result. Keeping the filter clean can prevent microbial growth. Besides, impure solvents can also lead to plugging. To avoid this problem, you should use solvents with at least a 10-20 percent organic solvent.

Another common problem that causes pump failure is a stuck check valve. A sticky check valve can be removed and cleaned. If the pump still is not working, you may need to replace the check valve. For a general overview of this topic, you may need to check out this post: https://en.wikipedia.org/wiki/Metering_pump.

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Chromatography Vials

Chromatography vials are used to measure a sample's concentration. Vials can be glass or plastic. Each type has its own specification. The most popular types are made of polypropylene or polymethyl pentene, which are both translucent and have high heat resistance. Plastic vials are more affordable and are also very light weight.

Chromatography vials are available in preassembled sets or in separate pieces. They come in screw, crimp and snap finishes. Some have crimp tops to create a tighter seal while others have flat or beveled finishes to make it easier to remove. There are even pre-assembled kits that contain chromatography vials, closures, and septa. Follow this link to learn more about chromatography vials.

Chromatography vials can be made of glass or polypropylene. Glass vials are best for non-polar compounds, while silanised glass vials are best for polar compounds. Polypropylene vials are suitable for highly polar compounds. The type of vial to choose depends on the type of sample and its volatility. In addition, the type of autosampler used in the experiment will affect the type of vial.

Chromatography vials can be made of clear or amber glass. Amber glass vials have a greater UV protection than clear glass, which can cause damage to certain chemicals. Amber vials also provide the best visibility. The size of the cap also matters a great deal. Especially if the sample is handled with a robotic arm machine, it is recommended that the cap be 9 mm or larger.

Increasing importance of HPLC test and food safety concerns will drive the growth of the chromatography vials market. However, stringent government plastic usage norms will hamper the growth of the plastic chromatography vials market. The growing number of diagnostic and research centers is expected to drive the growth of this market. Click on this link: http://www.chromtech.com/ to educate yourself further about the topic.

Chromatography vials are temporary containers used for storing chemical substances in GC and HPLC instruments. Tisch Scientific offers a wide selection of vials with ISO9001:2015 quality certification, to ensure that your samples are accurately analyzed. They are frequently used in legal, forensic, and manufacturing environments.

If you're planning to purchase chromatography vials, make sure your labware service provider understands your facility's process and goals. Choosing a partner with extensive experience in the industry will help make the entire process much smoother. Once you've chosen the right partner, they will procure the necessary materials. They'll source chromatography vials from a preferred manufacturer. To familiarize yourself with this topic, read this article: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography.

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HPLC Pump Care and Troubleshooting

For your HPLC pump to work correctly, you need to know how to care for it properly. The first step is to check for any errors or malfunctions. Errors such as pump head missing, temperature limit exceeded, and pressure leaks can occur if your pump is not installed properly. Other possible problems include a damaged piston or pump drive assembly, or a partial mechanical blockage in the pump.

Another way to ensure that your HPLC pump is operating efficiently is to change the seals regularly. Changing the seals regularly will prevent leaks and extend their life. Additionally, if you're using a caustic mobile phase, it can cause damage to your pump's seals. Changing the seals will also extend the life of the pump's column. Browse this site: http://www.chromtech.com/ for a deeper understanding.

If you see a fluctuating pressure level, it's a good idea to perform a series of tests on the system. If the readings are unstable, the problem may be in the pump or detector lamp. If you're not sure, use a logbook to track maintenance and parts usage. You should also schedule preventative maintenance routines to minimize unproductive downtime.

While HPLC technology and methods have improved significantly in recent years, issues can still occur. A leak in the pump head may cause abnormal pressure. It can also mean that the controller is not setting the correct pressure. A broken piston or meter can also cause pressure issues. It's important to clean the pump regularly to avoid problems like this.

Regularly checking the inlet filter is another important aspect of pump care and troubleshooting. If it's not clean, it can result in cavitation, which can lead to flow loss in the pump. The filter can also become blocked due to microbial growth or impure solvents. In addition, you should make sure to degas the mobile phases prior to using them. You can also filter and cover the mobile phases to prevent microbial growth. For information relating to the topic, read about Chrom Tech today!

If you have a HPLC pump that isn't performing as expected, it's important to understand the proper way to take care of it. The best way to do this is by following the best practices recommended by HPLC manufacturers. This ensures that your HPLC will run smoothly for many years to come.

If the pump's check valve is sticking, sonication may be necessary. This will flush out any slack that is causing your pump to stop functioning properly. If this doesn't work, a replacement may be required. Depending on the type of check valve, this can be a simple fix.

If your HPLC pump is making noises, you should check for loose connections and leaks. You should also check for salt buildup. If you've gotten a leak, check your system for air pockets, voids, and any other oddities. Explore more on this subject by clicking here: https://en.wikipedia.org/wiki/Monolithic_HPLC_column.