#nuclet

[mesuchmax]

i decide to cancel my comic cuz it wasn't well receives so

i turn it into a game instead

[meatballmeatballspaghetti]

I really hate woody’s roundup/the woody collective because I would understand if it was objectively bad/evil blogs (endless hate, harassment, actual illegal bullshit) but what constitutes a “terrible fucking blog that deserves to die” is really skewed on tumblr. I mean, from what I’ve observed, nucletic-asshole deactivated from that papatulus fiasco, and now they’re just kinda like “oh look because they’re now part of the woody collective they must’ve been a terrible person. Its the worst form of callout culture, for getting an old url stolen now marks you as what many take as a "nazi”, or at best a bad person. Now they’ve devolved into typical funnyman bs where any response is hailed as godsend. Also, they made a post calling others to action so i guess as long as someone hates you for no good reason and you decide “enough tumblr for me” and you deactivate, your blog will be paraded around as a part of one of the dumbest tumblr trends of 2017.

[cancersfakianakis1]

High performance of targeted next generation sequencing on variance detection in clinical tumor specimens in comparison with current conventional methods

Abstract

Background

Next generation sequencing (NGS) is being increasingly applied for assisting cancer molecular diagnosis. However, it is still needed to validate NGS accuracy on detection of DNA alternations based on a large number of clinical samples, especially for DNA rearrangements and copy number variations (CNVs). This study is to set up basic parameters of targeted NGS for clinical diagnosis and to understand advantage of targeted NGS in comparison with the conventional methods of molecular diagnosis.

Methods

Genomic DNA from 1000 Genomes Project and DNA from cancer cell lines have been used to establish the basic parameters for targeted NGS. The following confirmation was conducted by clinical samples. The multiple variants tested by amplification-refractory mutation system (ARMS), fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) were evaluated by targeted NGS to determine the sensitivity. Furthermore, the multiple variants detected by targeted NGS were confirmed by current conventional methods to elucidate the specificity.

Results

At sequencing depth of 500×, the maximal sensitivities on detecting single nucletic variances (SNVs) and small insertions/deletions (Indels) can reach 99% and 98.7% respectively, and in 20% of cancer cells, CNV detection can reach to the maximal level. The following confirmation of the sensitivity and specificity was conducted by a large cohort of clinical samples. For SNV and indel detection in clinical samples, targeted NGS can identify all hotspot mutations with 100% sensitivity and specificity. On ALK fusion detection, about 86% IHC-identified cases could be identified by targeted NGS and all ALK fusion detected by targeted NGS were confirmed by IHC. For HER2-amplification, 14 HER2-amplification cases identified by target NGS were all confirmed by FISH and about 93.3% of Her-2 IHC (3+) cases were identified by targeted NGS. Finally, the targeted NGS platform developed here has accurately detected EGFR hotspot mutations in 215 NSCLC patients.

Conclusions

DNA from cancer cell lines is better than standard DNA as a reference to establish basic parameters for targeted NGS. Comparison of the conventional methods using a large cohort of patient samples confirmed the high preformance of targeted NGS on detecting DNA alterations.

http://ift.tt/2wbtqzs

[mesuchmax]

Second protaganist of Nuclet stories in the world of average heroes and villains

JerkGirl (Simon MintyLady) - The Asshole Heroine/ Nightmare Behemoth Age: 28 Gender: Female Kind: Human Occupation: Hero, Gardener Birthday: 1994 Zodiac: Taurus Chinese Zodiac: Dog Blood Type: B Height: 202cm Weight: 80kg Hair Color: Average Brown Eye Color: Average Black Favorites: Reading books, fly up to sky, playing video games. Abilities: Flying/Levitation, Superhuman Physical

A heroine called herself JerkGirl because of her attitude, and she attended that way she have seen so many heroes being nice in the front of people, even though she’s bitching on everyone, they still loves and rooting for her, which makes her confused and disappointed. She used to be a normal girl back then with flying ability only, then the incident happen and she lost her memory, but she gained a new strength and appearance, along with different personality.

[mesuchmax]

whoops forgot to mention another new chapters of nuclet, 3 in fact and that’s the conclusion of Volume 1 folks! X

[mesuchmax]

alright folks it’s time to make some details about the characters, well i mean couldn’t just left them being too dusty and abandon

so here’s the first character i made long time ago

Trink Trinket - The Retired WItch Villain/ Wanted Herb Shopkeeper Age: Unknown Gender: Female Kind: Witch Occupation: Herbalist Birthday: Unknown Zodiac: Pisces Chinese Zodiac: Rabbit Blood Type: Minty Blue Height: 142cm Weight: 55kg Hair Color: Holy Blue Eye Color: White Purple(Corneas) Calm Blue(Pupils) Favorites: Sleeping

A retired grand evil Witch and started Her herbal job to ease Herself from stress, She’s always being grumpy and angry all the times, the witch hunters are still trying to hunt Her down but always failed because She’s really strong and have Her godly overpower creature pet Traga to beat them up. She never have any friend or family until Tenegulum the Mage shows up and ask Trink to take her as a disciple, instead She take Tenegulum as Her own daughter to work for Her.

alright more stuff like this comin’ up very soon so yeah be prepare for annoying oc bio to you dash

[mesuchmax]

heya folks its been another while, another chapter is here! X

[mesuchmax]

oh geez sorry for the long wait folks!

here is the 6th chapter! have fun folks! X

[mesuchmax]

chapter 5 is here X

being quite busy and very unproductive about art lately, this probably common if you’re nearly the middle of the year

[mesuchmax]

some upcoming weird characters for upcoming chapters

[mesuchmax]

chapter 3 is here! X

oh yeah forgot to mention that theres 4 session in 1 story so

i guess it doesnt matter whos the real protagonist in this story

[mesuchmax]

Nuclet Update = New Chapter! Introducing Trink’s new company, Tenegulum!

Right here folks! X

[mesuchmax]

I made a comic! At least I tried though.

I did this like the start of 2016 on DA but they screwed it up and probably the reasons I left.

I’m not sure if some of you saw them back then but I remade everything and add some pages on the first chapter.

Read here pal, have fun!

[mesuchmax]

heya been way too long folks!

sorry for the lack of contents so here’s the new chapter of nuclet X