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Graphs are tough! ;(((

Here's a supplemental video describing a computer language (R) that will aid in simplifying your data.

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Are you interested in the NF-kB and STAT3 pathways?

Look no further...Here is a PDF version of Curcumin's role in future studies; how it inhibits these pathways and what effects it carries on Hodgkin's Lymphoma cells.

https://onlinelibrary.wiley.com/doi/epdf/10.1002/ijc.23477

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Definition of a B cell via NCI's Database

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Some faces of Vitamin C

🍊🍋‍🟩🍋🥦🍅🥝🫑🥭🥦🥔🍓🍌

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Acknowledgments

Special Thanks to the Following:

NUTGAP coordinated by Dr. Gerardo Mackenzie and Dr. Diane Beckles, Dr. Carole Hom, Bryshal Moore, Rosa Fragello, all presenters/faculty & staff of UC Davis and allied outreach, & UC Davis Nutritional Biology Department

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Outline of The Role of Ascorbic Acid on Hodgkin's Lymphoma

Introduction

Hodgkin’s Lymphoma(HL) is a blood cancer affecting the lymphatic system

Tumors are mainly characterized by formation of Reed-Sternberg cells (H-RS)

HL has hyperactivation of the NF-kB pathway, which H-RS cells depend on for growth and survival

Ascorbic Acid (Vitamin C) acts as a antioxidant, yet in highdoses reached by IV, can act as a prooxidant which selectively kills cancer cells

Objectives

To investigate mechanisms describing how Ascorbic Acid (Vitamin C) kills Hodgkin’s Lymphoma

To visualize growth suppression & apoptosis (cell death)

Methodology

Cell Culture/Stock

Cell Proliferation

Immunohistochemistry

Antibody Probing

Cell Viability Assays

Image J Quantification

Results

Fig 2. Immunohistochemistry staining for PCNA and Bcl-xL were performed in L540 xenograft sections. Photographs were taken at 20x magnification )Below). Results were expressed as percent of PCNA+, and Bcl-xL+ area (mean ± SD) per 20x field.

Fig 1.Tumor Growth progression following treatment with Ascorbic Acid (AA)

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Fig 2. Images of Xenograft staining sections up close for PCNA and Bcl-xL

PCNA (+)

PCNA (-)

Bcl-xL (+)

Bcl-xL (-)

Conclusions

Tumor growth was inhibited when treated with ascorbic acid by a rate of 59%

Bcl-xL percent area showed no significant difference in staining when compared to control group

PCNA percent area showed no significant reduction in comparison to control group

L540 cells were revived, but were not viable for future experiments

Future Research

Determine the efficacy of Ascorbic Acid when combined with other chemotherapeutic drugs

Test if Curcumin (a tumeric bioactive) can effectively be combined with Ascorbic acid

Test Curcumin + Ascorbic Acid

Evaluate detailed mechanisms of action using HL L540 cells (using Western Blot)

References

1. Böttger F, Vallés-Martí A, Cahn L, Jimenez CR. High-dose intravenous vitamin C, a promising multi-targeting agent in the treatment of cancer. J Exp Clin Cancer Res. 2021 Oct 30;40(1):343. doi: 10.1186/s13046-021-02134-y. PMID: 34717701; PMCID: PMC8557029.

2. Guorgui J, Wang R, Mattheolabakis G, Mackenzie GG. Curcumin formulated in solid lipid nanoparticles has enhanced efficacy in Hodgkin's lymphoma in mice. Arch Biochem Biophys. 2018 Jun 15;648:12-19. doi: 10.1016/j.abb.2018.04.012. Epub 2018 Apr 18. PMID: 29679536.

3. Mackenzie GG, Queisser N, Wolfson ML, Fraga CG, Adamo AM, Oteiza PI. Curcumin induces cell-arrest and apoptosis in association with the inhibition of constitutively active NF-kappaB and STAT3 pathways in Hodgkin's lymphoma cells. Int J Cancer. 2008 Jul 1;123(1):56-65. doi: 10.1002/ijc.23477. PMID: 18386790.

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Thank you for stopping by!

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Understanding xenograft model💉

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Let’s understand the term inoculation.

Inoculation is a fancy word for Injection of pathogens, viruses, and other microbes into an organism.

In science, there are certain organisms that closely match the human biological system.

We call these model organisms.

One common model organism used in research is a mouse.

Mice studies have been used and are still being used within the scientific community for study of infectious disease, life cycles, psychological tests and much more.

Here is a visual of what types of inoculation look like. Scroll down to see a picture of a xenograft model that has underwent inoculation.

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Visuals of Lymphoma symptoms

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Cell Culture !!!!🫶🏽

🦠🧬🧫

Fun Fact: Flasks were used in the the ascorbic acid study for maintenance of L540 cells!

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Check out this Curcumin Study by Gerardo Mackenzie:

TEST YOUR KNOWLEDGE

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