#STEM labs setup

[ruiniel]

Reading another way has gave me an au idea that's driving me insane in the best and worst way possible

What if instead of alucard coming to our world, a reader from our world gets transported right into Dracula's lab room. Reader is a STEM (chem/physics leaning) major and starts panicking because not only are they in a strange room, but they're in a room that will literally cost them a fortune to repay if the damage anything (they don't even need to be American, lab equipment is ridiculously expensive all over the world) but Lisa walks into the lab to get anything she needed from there before heading to Wallachia and finds reader(same age as alucard) and the whole Isekai language barrier shenanigans endue but this time with a bit more spice cause reader know some formulas and blueprints that are needed for some advanced technology.

Some basic notes: Lisa lives cause she takes reader under her wing, Dracula stays happy and sane though he did think reader was dumb at first from their panicked expression in his lab (he thought they were of the "WITCHCRAFT" people) alucard now has a new housemate that he's learning to communicate with and share hobbies with.

Anon this sounds like an awesome fic idea, and there's the whole family to add into the story (wohoo) with some interesting results, I think! Because building them as characters interacting with Reader also means Reader automatically gains more dimension bc they're forced to 'act' differently around each character, so there are more interactions (hence scene possibilities) to have fun with I could think of:

1. Reader & Dracula

2. Reader & Lisa

3. Reader & Alucard

4. Dracula & Lisa

5. Dracula & Alucard

6. Lisa & Alucard

Maybe, maybe even uh... Could add something from the show / Reader arrives before Lisa's death anyway so up until that time a lot of things can happen but maybe at one point that 'visit' from the bishop has to happen as it does in the show, could maybe go differently if Reader were there hmm? Or if Reader had some way to tell Adrian to get over there please-now and the Church doesn't get to burn her home clinic to the ground or capture her - can also be a great bonding moment for Reader and Alucard (or maybe the Church does take her and they break her free)

Oh, oh, and with such a setup it could also be a possibility to explore the vampire world via Dracula, since the family's all happy and together ^^ (more trouble can happen from anywhere, like maybe Dracula's faced with a conundrum involving other vampire factions or something similar / and doesn't want bloodshed and Reader&A figure out a way?) I'm riding the idea train here with no end in sight lmao but the prompt is great, especially for the Drac&Lisa live part! Really nice! Someone here requested a reader-falls-into 1470s Wallachia too, wrote a oneshot but R was on the Humanities side there and landed in pretty much the middle of Forest 😄

Feel free to share more! Love getting these

[letsdothisbruh]

I posted 44 times in 2022

That's 44 more posts than 2021!

24 posts created (55%)

20 posts reblogged (45%)

Blogs I reblogged the most:

@cybersugarstuffs

@stuck-with-that-sum

@studyandstorms

@sizeleak

I tagged 22 of my posts in 2022

Only 50% of my posts had no tags

#letsdothisbruh - 21 posts

#motivation - 21 posts

#study - 21 posts

#studyspo - 21 posts

#studyinspo - 21 posts

#studyblr - 21 posts

#100 days of productivity - 20 posts

#we can do it - 19 posts

#stem major - 19 posts

#together - 18 posts

Longest Tag: 24 characters

#100 days of productivity

My Top Posts in 2022:

#5

Day 1/100- Nov 14- Mon

Trying to flesh out my SOP from the edits I got from my folks… it’s been daunting since I’m unable to find a good framework narrative without it sounding cliched…

Planning to put together a ppt and meet my advisor today, let’s see how that pans out. I have been anxious about it and have been procrastinating it for a while now.

Hopefully I make it through today and am productive.

Just reading a paper right now to finesse my question of purpose.

Song of the day:

On repeat 🔂

2 notes - Posted November 14, 2022

#4

Day 10/100- Nov 23- Wed

Half the week is over and I feel lazy and useless… gosh damn it.

I didn’t feel like doing anything today but still made myself come to my lab for the microscopy time I booked since it’s super crowded and I can’t try to change timings or make it up later…

I’m hoping to go back home and just finish some of my tasks for my courses. Today. I have to work it.

My avengers travel mug is keeping me sane with caffeine for now….

Song of the day:

This song accurately describes how I’m not enough how much ever I keep trying to jump hoops for everyone’s expectations of me 😑

2 notes - Posted November 23, 2022

#3

Day 17/100- Dec 2- Fri

Why is time moving this fast?

I didn’t get much done in terms of studying today but got much lab work done, setup for the genetic crosses for the week and made sure I did all I can so I can stay home guilt free tomorrow and go in on Sunday instead.

Even took time to go see my prof today and phew, I can see a tonne of work brewing up for this weekend!

The trick I need to master here is to hit the bed right now and make sure I wake up at a normal morning time tomo, eat healthy and NOT be intimidated by the lots of tasks… just gotta… bunch it all up into smaller tasks and get going and finish it!!

Just trying to get the courage to work smart and hard in the next few days, but I will be fine. I have to!!

Song of the Day:

4 notes - Posted December 2, 2022

#2

Day 12&13/100- Nov 26- Sat

Hmm I seemed to have forgotten to post yesterday..

Eh no one cares anyways.

See the full post

6 notes - Posted November 27, 2022

My #1 post of 2022

Hey I found the #studychallengewithcleo by @that-premed-student and decided to go for it!

Today I’m continuing to work on my Thesis committee meeting presentation right now. Been at it since morning and I’m sure I crossed the 6 hour mark a while back haha

I’m definitely taking breaks in between, and eating food and drinking water/beverages (do be kind to yourself and take care of yourself even if you are stressed)

So currently my work for today is:

Finish analysing my final dataset

Include those graphs on my ppt

Finish including all the info from the tagged papers on my ppt

Make a script and read it 3-5 times before retiring for the night

And ofc overthinking and writing down any Qs I could be asked in that meeting tomorrow and looking up and keeping track of those answers separately.

I think my list is doable… I am hoping my presentation tomorrow goes amazingly well and my committee members greenlit me for my final semester without any issues.

8 notes - Posted November 29, 2022

Get your Tumblr 2022 Year in Review →

[shimbongulus]

Day 17: Alphys

(oh hecc i almost forgot it was alphys day! Read the rest of the story here)

Subtly, Toriel’s body language changed from a more standoffish and cautious tension to a softened stance as she grew to comprehend Flowey’s situation.

“Well, you and Frisk take care of yourselves while I am gone. I must arbitrate a dispute,” she spoke as she finished watering her plants.

As she went for the gate and opened it, a familiar yellow shape appeared, in her usual sweatpants and tee-shirt. “Oh! Good morning, Alphys,” spoke Toriel. “H-Hi, Toriel,” stammered out the yellow lizard. “Going to deal with that… situation?”

Toriel nodded firmly, “Yes, that small white dog has me at my wit’s end. If it causes any more crashes, I will have to do something. Enjoy your day!” The boss monster departed as the lizard awkwardly stared at the flower.

“Oh… H-Hi..”

Flowey grinned. “Hey, mommy,” he intoned with a caustic tone. “Here to check up on your kid?” Alphys looked at the ground for a moment, “A-Actually, yeah. I wanted to, to, uh, ask if you’ve been feeling okay, since, uhh…”

The flower could barely stand the lizard’s stammering, it annoyed him to no end! “Gosh, you’re pretty nervous about seeing one of your greatest creations! I’m doing fine, if you couldn’t tell.”

Alphys rubbed her claws together, before she gathered something up and raised her eyes to meet Flowey’s. Her face screamed of guilt. “I… I know I shouldn’t have brought you to life without your consent.” She paused, “Uh, not that, uh, you could, well, consent, to, uh, being, created, without, uh, existing first, but, uh…”

“God, can you just spit it out?”

Alphys complied as she spoke, “Uh, anyways, I figured the least I could offer to do is to offer to… check on you. If you want, I can look at you back at my setup-”

Flowey’s face soured decisively, “Uh, no thank you, at least not at your lab. I don’t want to risk getting any more of what you gave me the first time.”

The lizard’s face turned downward slightly as she noted, frowning, “That’s fair.”

The flower turned to her, “But, uh, I’d be fine if you wanted to… just… look at me. Just, no needles or anything.”

Alphys cheered up a bit as she came towards Flowey. She examined Flowey’s stem and leaves, “Huh, so far so good. Looks like you haven’t, uh, gone like any of the others.” She continued as she examined the flower’s face.

For fun, Flowey morphed his face to appear as if it were melting, “Uhh… Doc…” Alphys began to panic, her claws shaking, “Oh, oh, dear, uh,” as she quickly began to withdraw something from her bag. She stopped only when she heard the coarse sound of Flowey’s laughter, “Hee hee hee hee heeeeee!” She turned and saw the flower’s face, completely normal. “Oh, so easy, so easy..”

Alphys’ face curdled. “Ha, ha,” she grimly replied. She withdrew an electronic device. “Would you be okay if I checked how things are in there? Just, non-invasive, real quick?”

Flowey took a moment. He was confident enough, however, that Alphys could probably put two and two together already well enough. “Eh, sure.”

Alphys took the scanner and passed it along the main of Flowey’s stem. “Huh. Okay, seems empty enough. There’s some odd, static-y action going on in there.” She banged her scanner a bit, “Might just be this thing going on the fritz, picking up some residual magic from Toriel’s kitchen or something. Egh, stupid phasic interference.”

Alphys turned to Flowey as she started up, briefly, “It’s the worst, because just when you think you’re getting a stable reading, an unsecured window reminds you that your neighbors are smoking something with fire magic! It’s the most -” Flowey stifled a yawn as Alphys caught wind of Flowey’s disinterest. “Uh, eh, uh, right. Anyways, everything seems all right.”

There was an awkward moment of silence. “So, uh, let me know if anything.. Changes…”

“Sure thing, mom.”

Alphys’ face curdled again as she shuffled out of the garden. The house now consisted solely of Flowey and Frisk.

[stewardofningishzida]

Real-Life Scary Story 7:  Dangerous Equipment 

TRIGGER WARNING: Almost getting electrocuted

I’ve worked in 3 different labs over the years so far. The first one was nice. Almost homey because it was fairly small and basic. It was where I first learned the foundational skills for laboratory science and research. The third lab was well-funded and had some of the latest equipment in it. My mentor and the staff there were nice; much like the ones in the first lab. The second lab…left much to be desired. You could tell that they were trying their best, but there was simply too much going wrong at once and everybody there was frustrated and miserable. This story is from the infamous lab number 2. The same one I burned my eyes in. (See the previous story.)

In this second lab I was in charge of maintaining the lab’s stem cell colonies, working on my own project, and helping with the PI’s (Principal Investigator’s) project. Besides growing cells, I also had to integrate specific ratios of chemicals called “growth factors” into the cells’ growth medium, the liquid they live in. These chemicals helped the cells “decide” the kind of tissue they were going to grow into. As an added twist, I also needed to put them in special dishes with a nanoscale scaffold inside that I made myself with a technique called “electrospinning”. This scaffold held the cells and put a specific amount of support on them to push them further into the right tissue type I wanted.

With electrospinning…Think of it almost like Spider-Man’s web launcher, but to get the tiny fibers (thinner than a human hair!) to make the scaffold I wanted, I had to run a strong electric charge through a metal plate to attract the fibers together on it. The fibers come out as a liquid polymer (very long molecule) through a needle and it dries very quickly. So, to prevent it from getting stuck on the end and ruining the needle, there’s a powerful electric current in the chamber the setup is mounted inside of. The polymer has a positive charge and the metal plate has a negative charge. So, the current helps the polymer go to the plate and dry there. There are machines to do it all now, but when I was working there, the lab couldn’t afford such an expensive machine. So, I had to do it the old way: Sitting there and adjusting the current while making sure the needle didn’t clog and turn it off when the scaffold was finished. This would take hours every day.

Note that this old-fashioned rig was haphazard at best and consisted of a tent surrounding a large aquarium tank with the needle mounted inside it on one end, some clamps to hold the metal plate inside it on the other end, a wooden stool in the tent to watch the machine, a short wooden pole with a paper towel on it to wipe the needle clean, and the charge generator itself next to the aquarium tank and the stool. There was a small pipe coming out of the top of the tank and tent to funnel out the toxic fumes from the polymer. I would sit on that stool adjusting parts of the machinery for 3-4 hours every day. The charge generator would usually run between 4000-6000 volts and a fairly lethal level of amperage. It’s not voltage that kills people, mind you. It’s the current. Amperage. The gauge on the generator for amperage broke about a month into the experiment, but before it did, the display registered a fairly decent current.

So, one day, I go in to build more scaffolding for my experiment. The other lab members were taking inventory and noticed that the freezer had frozen over. It had to be manually defrosted because it was an old model. They proceeded with it, but forgot that I was in the electrospinning tent. Water from the freezer leaked all over the floor…including into the tent. With the powerful electric generator on. Complete with exposed metal parts. I noticed the water and crawled onto the stool to keep my feet dry. Then, I called out to warn them. When they realized what was happening, they immediately started mopping the floor and brought in some fans to dry the area. I had to finish the scaffolding because it would be completely ruined and delay the experiment if the machine were turned off. That would have cost the already underfunded lab thousands of dollars that it didn’t have. So, while my labmates attempted to contain the mess and stop the water from flooding the tent more, I ended up trapped on top of the stool for 2 full hours. I have to say, working with the looming threat of electrocution if your feet touch the wet floor is a surreal and terrifying encouragement.

After the water had finally dried, I finished my scaffolding and took a well-earned lunch break. The labmate who had accidentally let water leak into the tent begrudgingly agreed to build the next set of scaffolding for me. I didn’t report them at the time because I was a lowly intern afraid of losing my position. However, a few weeks later when the safety inspector came, we got temporarily shut down for safety violations. Makes you think, doesn’t it? There were a few other unpleasant experiences in that lab as well. So, when my internship there ended, I packed up and left as if the place was on fire. I learned a lot there, but I was happy to be done.

[raymondqvma076]

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[nadziejastar]

i hope this question is alright. i noticed that what broke isa’s character for me when saïx was given agency is that his berserker mode no longer made sense. rather than seem like pent up rage against his abusers, it seemed to just be saïx again being a total douchebag. saïx always came off to me as someone with ptsd, maybe c-ptsd and suddenly giving him agency against xemnas and the apprentices is a strange take. i can elaborate more but the 300 character limit is brutal lol!!

i hope this question is alright.

Perfectly alright.

i noticed that what broke isa’s character for me when saïx was given agency is that his berserker mode no longer made sense. rather than seem like pent up rage against his abusers, it seemed to just be saïx again being a total douchebag.

I completely agree. Saïx’s characterization in KH3 was nothing like I was expecting based on all the past games. It was totally incongruous with his depiction in all the other media (games, novels, manga). It was ridiculous. I was completely baffled over why he was acting the way he did, why he was apparently completely in control of himself during his fight, and why he was being treated like a totally normal guy, when he obviously was not.

saïx always came off to me as someone with ptsd, maybe c-ptsd and suddenly giving him agency against xemnas and the apprentices is a strange take. i can elaborate more but the 300 character limit is brutal lol!!

Feel free to elaborate as much as you want if you feel like you have more to say. But from what you’ve said in the 300 character limit, I couldn’t agree more. Saïx absolutely seemed to have PTSD to me, too. I would say Axel did, too. Something very awful happened to them both, leading them to join the organization. Though I think Saïx had gotten the worst of it. Axel’s anger seemed to stem primarily from the way Isa was so abused. It’s why he had the patience of a saint with him. I don’t know exactly what happened to Saïx, but it sure as hell wasn’t him becoming an apprentice to look for Skuld.

I also found it really weird that Xemnas showed up as soon as Saïx stopped attacking Lea. Seriously. It was instantaneous. Since the Norts were all supposed to share a heart and consciousness, it actually makes sense. But oddly, Xemnas had no reaction whatsoever to Saïx just standing there refusing to fight Lea. He didn’t scold him or call him a traitor or anything. He didn’t acknowledge him whatsoever. It was downright bizarre. To me, it’s just evidence that Saïx’s fight got drastically rewritten at the last minute, but they left certain things the same, making it into nonsense.

Lunacy: A term describing insanity, once considered to have been due to changes of the moon, now used legally and colloquially but not by clinicians. In the middle ages, the symptoms and behaviour of those with mental disorders or defects were often ascribed to demonology or witchcraft, and some sufferers were cared for by monastic orders.

Belief in the “lunar lunacy effect,” or “Transylvania effect,” as it is sometimes called, persisted in Europe through the Middle Ages, when humans were widely reputed to transmogrify into werewolves or vampires during a full moon.

It really, REALLY bothered me that everything cool and interesting about Saïx—the berserk mode, the lunacy, his vampire-like design and personality, the X-shaped scar—all of it has been entirely removed from any story context. It’s all just random aesthetics that have no meaning now. He’s just a normal guy who was kind of a douchebag. That’s it. That’s what he was reduced to.

NOBODY in good faith could possibly argue that Saïx’s concept and design don’t fit the backstory of a test subject waaaaaaaay more than his canon backstory. It’s literally impossible. Saïx’s lunacy fits being a human lab rat. I suspected that he and Axel were test subjects even in the original KH2. Because it just fit that well.

Honestly, it pissed me off that apparently, we were supposed to see that Saïx was only pretending to fight Lea to the death, in order to keep up appearances. He needed to seem loyal to Xemnas so he could bring back Roxas or whatever.

It’s honestly the stupidest thing ever. I’m a big fan of the manga Berserk, so I enjoy reading up on the history of berserkers and such. And I find the idea that Saïx was just putting on an act the whole time to be ridiculous and it spits in the face of what it means to be a berserker.

Berserkers would slay anyone who got too close. Friend or foe. Isa wasn’t aware that he was fighting his best friend. That was the whole point. Isa’s heart was strong enough to regain some control before he killed Lea. That is very much in the spirit of this series.

Xemnas: My friends! Remember why we have organized–all the things we hope to achieve. The strength of the human heart is vast. Soon, though…we will have gained power over it! Never again will it…have power over us.

The heart is the most powerful thing in existence. All Xemnas wanted was to gain power over the heart. This was what he said while looking up at the giant heart-shaped moon. The same moon that causes Saïx’s lunacy.

Roxas: Love is a power?

Xaldin: None you or I will ever grasp. Nor will they, for long. The love between them will wither and die. Love never lasts.

Roxas: But you don’t have a heart. How would you know?

Xaldin: I have eyes, and a brain. We have no further business here. Try not to dawdle.

Roxas: That still doesn’t explain what love is… Is love fighting to protect what’s most important to you? Where does its power come from?

Throughout all of Days and KH2, Xemnas did have total control over Isa’s heart.  He turned him into a very powerful killing machine who felt like his only purpose in life was to become stronger.

I love Roxas and Axel. I’m sure Saïx would scoff at that. Call it a trick of my artificial memories. But the time I spent on that clock tower was real.

But he was incapable of the power of the heart—love.

Roxas: I found out about love on today’s mission–that it’s something powerful.

Axel: That’s true. It is. But I’ll never get to experience it.

Roxas: Nobodies can’t love?

Axel: You need a heart, man.

Roxas: Right…

Axel: Love is what happens if there’s something really special betweentwo people.

Roxas: You mean, like, if they’re best friends?

Axel: Well, you can care about your friends, I guess, but that’s not what I’m talking about.

Love is the greatest power of all. It’s a power even Xemnas cannot control. He has power over “nothing”. That was what Isa’s fight with Lea should have been about. Very simple.

It’s why Isa stopped attacking Lea when he looked into his eyes, just before he finished him off.

It’s why he was just standing there like a mindless zombie and the camera zoomed in on his face when Xemnas began attacking Lea.

It’s why Isa was the one shown standing there behind Xemnas right before he tried to finish Lea off. This was what Isa’s character arc was set up for ages ago. Ever since KH2FM+. Literally over a decade of build-up for this moment.

Saïx emotionally abuses Axel and his two friends for an entire game. Then, he spends the entirety of the next game trying to kill him, and succeeds at the end. Then, in the grand finale, they have a lukewarm reconciliation at the last minute. Does this feel like a satisfying and well-thought out story that fits in with the themes of Kingdom Hearts? NO!

The entire fight was supposed to be a dramatic demonstration of how much Isa LOVED Lea. He loved him so much he was able to break free of Xemnas’s control, to protect him. Nobody could ever convince me that Saïx/Isa had a good character arc in KH3. He didn’t. It was abysmal. I actually found it offensive, it was so bad. It’s because the setup for his story was so good and they turned it into such trash.

[crippleprophet]

Do you know any other blogs that post about being disabled in STEM fields? I'm studying ecology/biology/natural history, and I get really worried about being unable to find work or research experiences since I can't do most field work (chronic pain). Also, do you have any advice? I'm worried I'm just not cut out for the type of work I want to do.

unfortunately, i can’t think of any other blogs that post about bring disabled in STEM, although i follow a Lot of chronic pain blogs so it’s entirely possible that someone i follow has talked about it and i just didn’t see the post(s). if any of my followers post about being disabled in STEM, feel free to self-promo on this post or send an ask!

as far as being worried about finding jobs & research experiences, i really feel you - a lot of the engineering research labs at my school are too small for me to navigate with a mobility aid, and a lot of the work requires more walking/standing than i can do. that made me really worried during my freshman year, but then i was accepted to a 4-week research program over that summer & the program director’s partner is disabled (my forearm walker is actually a hand-me-down from her) and so we got to talking about accessibility and he invited me to work on a project with him. there’s another engineering professor at my school whose wife is a psychologist with CP and so he & his wife are collaborating on a study about a) the psychological effects of inaccessible classrooms & labs in STEM and b) how to improve accessibility and get more disabled students in STEM.

i bring up both of those anecdotes because like, things are really bleak a lot of the time, but i also genuinely believe there will be people who want to work with you. it’s been a year-and-then-some process for me to believe them, but my aforementioned research advisor & my mentor who i met through the same experience were both insistent that even though i couldn’t do the manual labor or field work that the other students were doing, my perspective was incredibly valuable because i approach things from a different frame of mind and life experience. there were a few examples where this came into play, but a big one was when my mentor met with me probably 8 months after the program to try to remember a specific detail about a water sample we’d collected. because i’d been sitting and note-taking on site since i couldn’t stand long enough to collect samples, i remembered what happened when no one else did and she got the records fixed accordingly.

in terms of advice, i’ve got a few things, in no particular order:

if you’re an undergrad, graduating spring semester 2021 or later, and have at least a 3.0, apply for National Science Foundation Research Experience for Undergraduates (NSF REU) programs for next summer. you might’ve already heard of them, but if not, they cover housing and either partial or full meal plans for a 9 or 10-week summer research program at various universities, plus you get a substantial stipend, and they specifically prioritize accepting women, people of color, and disabled students because those groups are incredibly underrepresented in STEM. i’m participating in an REU right now and it’s been a really good way to learn what works for me and what i won’t be able to do in a future job. also, consider this an open offer to any disabled folks reading this who are interested in applying to REUs that i’m totally down to answer any questions and review/edit application material if you want! feel free to send me an ask or dm me

make a chronic pain/illness management plan. i’ve been planning to make a post about this soon since i’ve been updating mine for next semester so i’ll get into it in more detail later, but it’s basically a breakdown of everything you do (i have school, work, extracurriculars, hygiene, food, room setup, and major events as my categories) and what you can do, what you can’t do, and what you need help with for each task. for example, i have that i can study or work in my room but i can’t meet people across campus to work on a group project at night, on weekends, or at the last minute. i can go to meetings for the student org i’m on exec for but i can’t meet with campus life without at least a week’s notice. and so on. i’m gonna share the plan with my roommates so they hold me accountable to not push myself too far because i trust them a lot, but if you don’t have roommates or aren’t close with yours, you could share it with a close friend or something

keep some sort of list of why you’re doing this. it doesn’t have to literally be a list; i keep a photo album on my tablet of snapchats i’ve taken during moments where i’ve been like, this is why i love what i’m doing, this is why i want to spend my life on this. because it gets really shitty far too often, and i’ve wanted desperately to change my major or drop out or just press pause on the world more times than i can count, and i’ve broken down crying with how fucking hard it is a lot. but i really, really do love this, so it helps to have something to remind myself that like, it’s not just a montage of me crying walking to class and crying in class and crying doing my homework because everything hurts; there are moments of such joy and victory, and there are things i’ve learned that have made me so indescribably thrilled, and i really do want to do this

distance yourself from toxic attitudes. this is of course easier said than done, especially if you’re in a small program, but i just have zero patience & energy for the folks in my classes who are constantly trying to one-up each other with how little sleep they’ve gotten and how long they were in the library and what grade they got and whatever the hell else. my body demands that i sleep a certain amount. my body demands that i rest when i’m not at a meeting or in class. i physically can’t push myself the way my peers do - and no one, not even abled people, should, tbh - and so the best way for me to not compare myself to them is to surround myself with chill and noncompetitive STEM majors or non-STEM folks who look at our entire toxic culture like ‘wtf y’all’ and to honestly just put some headphones in when my peers start talking about that sort of shit before class or whatever

go to therapy if possible. like honestly everybody, especially every student, needs to be in regular therapy, but obviously it’s not always an option and some schools have restrictions on how many sessions you can attend. mine doesn’t have a cap, so i go every 2 weeks, no exceptions. my therapists haven’t always understood certain aspects of chronic pain and disability and my perspective, but it’s been so vital for me to be able to just yell about ableism and how scared i am and how hard it is to live in pain all the time without having to worry about the other person’s reaction like i would with a friend

don’t compare yourself to abled people. which is obviously so much easier said than done, but something i keep in mind is: my freshman year, i was really overwhelmed by how everyone else who has my scholarship is doing more than me. they’re taking more classes, they’re involved in more organizations, they’re doing more events on the weekend, they’re working more hours, on and on and on, across the board, this is not an exaggeration. and this was before i’d started talking about disability in my sessions, but my therapist looked at me and said, yeah, but none of them are out as trans. none of them have to navigate constantly explaining their identity to people in order to have the right language used for them, constantly self-advocating, constantly navigating a space that wasn’t built for them - that’s exhausting. that’s work. & the same thing definitely applies to disability; taking care of this body is a full-time job on just a physical level, and then there’s self-advocacy and navigating ableist environments and cultures on top of it. so the experience of any of your abled peers is fundamentally incomparable to yours

i think that’s all of the main stuff. please know that i really feel your fear, it’s one i have on pretty much a daily basis lately, and i know this field of study seems determined to keep us out of it some days, but i’m still here through a mixture of a lot of love and a lot of spite. that being said, i fully respect any decision you make with regards to your life plan and i don’t begrudge anyone who decides STEM isn’t a healthy place for them to be.

my inbox & DMs are always open if you want to talk about this more 💙 i know it can be so incredibly isolating so i’m here if you need someone who gets it. i hope this helps & i wish you the absolute best

[literaturepublishersjournal]

Video Journal: Clinical Videos Journal and Medical Videos Journal

Video Journal: Clinical Videos Journal and Medical Videos Journal accepting articles with clinical videos, medical videos, surgery videos or surgical videos for publication. Journal covers the publication of new information by publishing original research from all areas of clinical research video journal, medical research video journal and basic sciences including clinical medicine video journal, clinical trials video journal, experimental research video journal, epidemiology, preventive medicine, translational medicine and rural health. Every written manuscript submitted for publication to Clinical Video Journal must be accompanied by single or multiple videos.

Journal Homepage: https://www.literaturepublishers.org/

Journal of Clinical Videos is a publication of scholarly work in a video format. Researchers get to demonstrate their work to the audience by using a live camera shoot. Since these medical video journals let researchers showcase their work in a more visual way, the impact of their research is increased.

Links of Published Clinical Video Articles

Journals in clinical video format are broadly grouped into scientific video journals; medical video journals; technical video journals; and arts, humanity and management video journals. Each of these are further available in diverse areas including medicine, science, technology, arts, humanities, biology, management, chemistry, physics, neuroscience, engineering, genetics, cancer research, immunology and infection, developmental biology, behavior, environment, bioengineering, biochemistry, psychology, clinical skills, and many more. Even under each of these specializations, there are different sub-specializations.

For instance, under medical video journals, there are videos of cardiology video journal, oncology video journal, neurology video journal, clinical ophthalmology video journal, biomedical science video journal, cardiothoracic video journal and vascular surgery video journal, internal medicine video journal, gastroenterology video journal, pediatrics video journal, pathology video journal, radiology video journal, surgery video journal, obstetrics video journal and gynecology video journal, otolaryngology video journal, orthopedic surgery video journal, pathology video journal, etc.

Open Access Clinical & Medical Video Journal

Open access video journals are available to readers, libraries, institutions, and organizations without any barriers like payments, licensing, subscriptions and copyrights. Academicians and scientists can freely access these journals to broaden their understanding of the current happenings in their respective disciplines.

Video demonstrations enhance reproducibility and productivity, aid better and quicker understanding of experimental methods and scientific concepts; disseminate research widely; save laboratory expenses; increase learning speed in lab and class; promote consistent growth in learning outcomes, student success and STEM retention. Thus the different types of video journals including medical video journals; scientific video journals; technical video journals; and arts, management and humanity video journals are a boon to the research, student and teaching communities. You can check out and make the best use of the medical Video journal available at the Clinical Images and Case Reports Journal.

Manuscript Submission

Authors are requested to submit their Clinical Video articles only by E-mail to the Editorial Office at: editor.cicrj@literaturepublishers.org

[rosesforwildwitches]

All of those are critical to science, and as a biochemist looking to go into research the anarchists have a hard time reckoning with that. but as an anarchist as well, I don't think that the current government or capitalist systems are helpful to scientific progress in the long term.

capitalism incentivizes bad lab practices, overwork beyond any reasonable situation that only serves as a detriment to lab work, and it prevents people from running checks or repeats of previous experiments to verify results, leading to a replicability crisis. due to the fact that government grants and corporate sponsors who fund labs are only interested in the new, advertisable experiments and many refuse to fund any replicate experiments, 33% of all medical experiments can not be reliably replicated by labs other than the original experimenters, meaning that a full third of our medical research lacks a sound scientific basis because no one's going to pay you to check that something's true. capitalism also contributes to a lack of scientific literacy in the public, as capitalistic media very frequently misrepresents or oversimplifies studies to fit whatever narrative gets more clicks on a news site.

And governments are even worse long term for science and it's progress as a whole. There is something to be said for the communication and laboratory infrastructure they facilitate, but many governments heavily restrict or censor research based on the social norms of the nation and the goals of politicians. Scientists who have tried to run honest academic studies on sexuality, gender, and the actual effects of drugs in ways that don't align with the goals of lawmakers to demonize certain groups find themselves underfunded and censored in public coverage. Scientists who try to go up against industries like tobacco or oil that funnel money into lobbying groups face half a century of resistance before someone will even publicly acknowledge decades of papers and work saying that these industries do more harm than good. governments do provide material support for scientists, but only so long as the scientists in question don't become too "difficult" and start undermining political talking points that have no basis in the truth.

I don't think anarchists without a scientific background really have all of the answers, and frankly I don't have many good answers either. As a discipline scientists and researchers need massive amounts of infrastructure to do what we do, and we need common standards to make any sense of our findings. we can't be anarchoprimitivists and expect to have any kind of quality of life or to learn anything more about the world we live in. but we can't keep abiding by statist and capitalist systems just because they provide some labs and some money because the harm these structures do not only to STEM professionals but to everyone around the world outweigh any possible good they do, as both the state and capitalism are built on the creation of winners and losers that's only made possible by disenfranchising, exploiting, and legally sanctioning certain groups in society deemed to be "lower" than those who gain the benefits. I don't know what system will work to adequately support humanity as a whole with the aim of equality and self determination. But I do know that decades and centuries of history and studies have demonstrated that capitalism and state authoritarianism are not a viable solution to the problems we face around the world. Those who came up with these systems had the hypothesis that a capitalist system and a centralization of state authority would increase quality of life and economic satisfaction. This hypothesis has been demonstrably proven false, so instead of stubbornly holding onto it we must refute it, scrap the old setup, and look into alternatives.

I cannot overexplain how anarchists seem to think you can safely rig makeshift bullshit like yes I have no doubt that someone with experience and the appropriate lab could produce a chemical but instead it's always some stupid shit with plastic tubing and cut up milk jugs literal meth lab style drug manufacturing. Heating apparatuses as likely to explode into flaming liquid and shrapnel as they are to warm someone's hands. Rarely a second thought for the entire practice of engineering, or material sciences, or chemistry, or manufacturing, or any of the dozens of disciplines which we have as a society spent centuries refining. And no appreciation for the extremely tangible benefits of standardization and mechanization. There is a reason we have people who design processes, and people who build machinery to execute those processes rapidly, exactly, and reliably. Inconsistency is not something you can afford when people's lives are on the line, delays are not something you can afford when people need consistency of care. The immediate critical future of entire sectors of your community cannot hinge on improvisations.

[moremedtech]

Human brain organoids implanted into mouse cortex respond to visual stimuli for first time

Human brain organoids implanted into mouse cortex respond to visual stimuli for first time. A team of engineers and neuroscientists has demonstrated for the first time that human brain organoids implanted in mice have established functional connectivity to the animals' cortex and responded to external sensory stimuli. The implanted organoids reacted to visual stimuli in the same way as surrounding tissues, an observation that researchers were able to make in real time over several months thanks to an innovative experimental setup that combines transparent graphene microelectrode arrays and two-photon imaging. The team, led by Duygu Kuzum, a faculty member in the University of California San Diego Department of Electrical and Computer Engineering, details their findings in the Dec. 26 issue of the journal Nature Communications. Kuzum's team collaborated with researchers from Anna Devor's lab at Boston University; Alysson R. Muotri's lab at UC San Diego; and Fred H. Gage's lab at the Salk Institute. Human cortical organoids are derived from human induced pluripotent stem cells, which are usually derived themselves from skin cells. These brain organoids have recently emerged as promising models to study the development of the human brain, as well as a range of neurological conditions. But until now, no research team had been able to demonstrate that human brain organoids implanted in the mouse cortex were able to share the same functional properties and react to stimuli in the same way. This is because the technologies used to record brain function are limited and are generally unable to record activity that lasts just a few milliseconds.

The researchers developed experiments that combine microelectrode arrays made from transparent graphene, and two-photon imaging, a microscopy technique that can image living tissue up to one millimeter in thickness. David Baillot/UC San Diego The UC San Diego-led team was able to solve this problem by developing experiments that combine microelectrode arrays made from transparent graphene, and two-photon imaging, a microscopy technique that can image living tissue up to one millimeter in thickness. "No other study has been able to record optically and electrically at the same time," said Madison Wilson, the paper's first author and a Ph.D. student in Kuzum's research group at UC San Diego. "Our experiments reveal that visual stimuli evoke electrophysiological responses in the organoids, matching the responses from the surrounding cortex." The researchers hope that this combination of innovative neural recording technologies to study organoids will serve as a unique platform to comprehensively evaluate organoids as models for brain development and disease and investigate their use as neural prosthetics to restore function to lost, degenerated or damaged brain regions. "This experimental setup opens up unprecedented opportunities for investigations of human neural network-level dysfunctions underlying developmental brain diseases," Kuzum's lab first developed the transparent graphene electrodes in 2014 and has been advancing the technology since then. The researchers used platinum nanoparticles to lower the impedance of graphene electrodes by 100 times while keeping them transparent. The low-impedance graphene electrodes are able to record and image neuronal activity at both the macroscale and single cell levels. By placing an array of these electrodes on top of the transplanted organoids, researchers were able to record neural activity electrically from both the implanted organoid and the surrounding host cortex in real time. Using two-photon imaging, they also observed that mouse blood vessels grew into the organoid providing necessary nutrients and oxygen to the implant.

Researchers were able to detect and image the border between a transplanted human brain organoid and mouse brain. Credit: Madison Wilson/UC San Diego Researchers applied a visual stimulus–an optical white light LED–to the mice with implanted organoids, while the mice were under two-photon microscopy. They observed electrical activity in the electrode channels above the organoids showing that the organoids were reacting to the stimulus in the same way as surrounding tissue. The electrical activity propagated from the area closest to the visual cortex in the implanted organoids area through functional connections.

The researchers observed electrical activity in the electrode channels above the organoids showing that the organoids were reacting to the stimulus in the same way as surrounding tissue. David Baillot/UC San Diego In addition, their low noise transparent graphene electrode technology enabled electrical recording of spiking activity from the organoid and the surrounding mouse cortex. Graphene recordings showed increases in the power of gamma oscillations and phase locking of spikes from organoids to slow oscillations from mouse visual cortex. These findings suggest that the organoids had established synaptic connections with surrounding cortex tissue three weeks after implantation and received functional input from the mouse brain. Researchers continued these chronic multimodal experiments for eleven weeks and showed functional and morphological integration of implanted human brain organoids with the host mice cortex. Next steps include longer experiments involving neurological disease models, as well as incorporating calcium imaging in the experimental set up to visualize spiking activity in organoid neurons. Other methods could also be used to trace axonal projections between organoid and mouse cortex. “We envision that, further along the road, this combination of stem cells and neurorecording technologies will be used for modeling disease under physiological conditions; examining candidate treatments on patient-specific organoids; and evaluating organoids’ potential to restore specific lost, degenerated or damaged brain regions,” Kuzum said The work was funded through the National Institutes of Health and the Research Council of Norway, as well as the National Science Foundation. More information: Madison N. Wilson et al, Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex, Nature Communications (2022). DOI: 10.1038/s41467-022-35536-3 Journal information: Nature Communications Source: University of California - San Diego Read the full article

[horsemains]

Making characters with magicavoxel

#Making characters with magicavoxel how to

#Making characters with magicavoxel update

The spraycan can be used to points of interest, while the fire extinguisher can put out fires. The player starts out with a sledgehammer and a fire extinguisher and spraycan. Different material require different tools to destroy. The player can freely navigate the game's nine open-world levels, which consist entirely of destructible voxels. Teardown is a sandbox game with puzzle and action elements. The player using an excavator to destroy a locked gate

#Making characters with magicavoxel update

The campaign was completed with a major update in December 2021, and the game was released in April 2022. It became available as an early access title in October 2020 and was met with positive reviews. After settling on the two-part heist format, Gustafsson announced the game as Teardown in October 2019. Teardown uses a proprietary game engine developed by Dennis Gustafsson, who, in conjunction with Emil Bengtsson, used it for several game prototypes. During the setup phase, which has no time limit, a path to complete these objectives as quickly as possible can be created by reshaping the game world. Each mission features a set of objectives to be completed within one minute. The game features levels made entirely of destructible voxels. If you want to use the normal Animator or Animation, either hide the blades inside the model until their are needed (which would make it somewhat realistic because after all, there is no magical "bag of holding" inside of a helicopter) or, if the parts are too big to hide, make (or rather their bones) smaller and only rescale them to their original size when they are redy to pop out.Teardown is a 2022 sandbox, puzzle and action game developed and published by Tuxedo Labs. The animator class is at the highest level and holds references to the (custom) animation clips a model has, and if seperate parts of the model are animated seperately as well (such as feet and body). The clip class holds information about the number of frames, and how much time between them passes. The frame class holds information about the mesh to use, any offset and rotation, and position and rotation of attachment points (for held weapons etc). What I did is write a custom animation system (sounds more complex than it really is), along with an editor extension that lets me create animation easily. I have implemented the second way you suggested (adapting the frame-by-frame animation od 2D sprites for 3D voxel-style models, as seen in 3D Dot Game Heroes). Currently I have the correct animation in MagicaVoxel but there is no way to export all of these different models to something that Unity would be ready to handle easily.Īm I overcomplicating this or has anyone ever done something similar and has some advice or helpful links? I have not been able to find much on this. It seems like the best way to accomplish this is through frame-by-frame animation similar to 2D sprite's.

#Making characters with magicavoxel how to

I want the blades and stem to rise out of the roof of the vehicle when doing a flying animation but I am not sure how to go about adding extra voxels to the model during this animation.įor example, I know that to animate the blades themselves on the model, I could rig the model in blender and then move them as an animation but how would I actually animate the stem and blades rising out of the top of the vehicle since they are a separate component and will not always be on the model? I have made a 3D all terrain vehicle in MagicaVoxel but I have run into a bit of an issue.

[high-clouds-stem-technologies]

High Clouds - Where ideas fly higher than clouds.

#HighClouds #Technoyugam #STEMeducation #STEMeducationInIndia #HighCloudsSTEMTechnologies 

[healthcare-domain]

The Future of Cell & Gene Therapy Manufacturing Services Market

The pandemic slowed the economic growth of various countries, including the US, Germany, the UK, India, and China. It also resulted in control measures that impacted operations in life science organizations, such as production and research. In an article published by Nature Biotechnology, the CEO of Flagship Pioneering (US) stated that lab productivity at many of its research-based setups was running at just 30–50% capacity, and even upholding that rate was a challenge.

More than 750 trials of cell & gene therapy (CGT) in almost 30,000 patients were underway as of June 2020, and CGT products account for some 12% of the pharmaceutical industry’s clinical pipeline and at least 16% of its preclinical pipeline. According to GEP Worldwide, more than 1,200 clinical trials across the globe were disrupted by June 2020. Nearly 61% of clinical trials were disrupted due to the suspension of patient enrolment.  

The increase in pharmaceutical R&D has resulted in a sharp increase in the number of cell & gene therapy candidates under development. This has made it necessary to outsource manufacturing services to develop cost-effective and efficient cell & gene therapies. 

Download PDF Brochure @ https://www.marketsandmarkets.com/pdfdownloadNew.asp?id=180609441

The market is primarily driven by the prevalence of cancer and other target diseases. The development of therapies for key diseases, such as cancer, has attracted significant attention. Furthermore, increasing investments of pharmaceutical companies in the development of novel drugs is also fueling the market growth. However, significant operational costs are expected to negatively impact the cell & gene therapy manufacturing market thus limiting the growth. 

Currently, there are 1,200 cell & gene therapies in trials worldwide. There are more than 700 investigational cell & gene therapies in clinical development in the US alone. However, manufacturing facilities have not kept up. It has been estimated that hundreds of facilities will be needed to manufacture the treatments that are now in clinical trials.  

One of the areas that need to be accelerated is viral capacity. Most viral vectors are produced using adherent manufacturing, which is expensive to operate—a vial of 20 million cells can cost USD 20,000 to USD 30,000 to make. The cost of manufacturing for gene therapy can be between USD 500,000 and USD 1 million, excluding the costs for R&D, the costs to run crucial clinical trials, or the costs to build the commercial infrastructure necessary to provide access to patients.

In 2020, cell therapy segment dominated the cell & gene therapy manufacturing services market. This can be attributed to the advent of new technologies and innovative products that have enabled several variety of cells such as hematopoietic stem cells (HSC), mesenchymal stem cells, natural killer cells, dendritic cells, and T-cells to treat diseases and conditions. 

Clinical trials are the backbone of medical research and help pharmaceutical and biopharmaceutical companies develop and commercialize new cell & gene therapies. In the past few years, the demand for clinical trials has risen worldwide as a result of the growing demand for novel medicines to fulfill unmet medical needs. According to a 2020 PhRMA report on the cell & gene therapy pipeline in 2018, there were 289 cell & gene therapies in clinical development by biopharmaceutical companies. 

Key Market Players

Key players in the cell & gene therapy manufacturing services market include Thermo Fisher Scientific (US), Merck KGaA (Germany), Charles River Laboratories (US), Lonza (Switzerland), Catalent (US), WuXi AppTec (China), Takara Bio Inc. (Japan), Nikon Corporation (Japan), FUJIFILM Holdings Corporation (Japan), F. Hoffmann-La Roche Ltd. (Switzerland), Oxford Biomedica plc (UK), and Cell and Gene Therapy Catapult (UK).