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Lab 8 - Biodiversity

Materials (Week 1) 

- Bacteria (Eubacteria) (Blue-green algae) 

- Protista (various representatives) 

- Plants (lower plants) (higher plants) 

- Fungi (Basidiomycetes) (Zygomycetes)

Materials (Week 2)

- Porifera (sponges)

- Cnidaria (Corals, Hydroids, Jellyfish)

- Platyhelminthes (flatworms)

- Annelida (segmented worms)

- Arthropoda

- Echinoderms 

- Chordata (invertebrate chordates) (vertebrate chordates - fish, amphibian, reptiles, birds, mammals) 

Methods

- recognize representatives for the major groups

- know the major morphological features of each group

- know very basic ecology of each group 

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Lab 7 - Genetic Architecture and Gel Electrophoresis

Ch. 5 & 9 

Genetic Architecture (ch. 5)

Materials

- envelopes

- colored tacks

- long pins 

- styrofoam head

- toothpicks 

- pipe cleaner

Method

Part I - Building Rebops

Part II - Finding your Rebop’s parents 

Part III - Mating Rebops 

Part IV - Karyology 

Gel Electrophoresis (ch. 9) 

Materials 

Part I - Procell electrophoresis apparatus, gel molds, combs, Power units, buffer and gels

Part II - Protein production through Transcription and Translation 

Method

Part I - Sickle Cell Anemia 

Part II - Transcription and Translation 

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Lab 6 - Biological Chemicals, Nutrition and Health

Materials

Part I

Carbohydrates:

- test tube racks, hot plate, 400 ml beaker, 3 test tubes, onion juice, potato juice, Benedict’s Reagent, distilled water, Lugol’s Iodine

Fats and Lipids:

- Sudan III, extracts of flour, cream, coconut, margarine, filter paper, pencils, dedicated Pasteur Pipet, water bath 

Proteins: 

- Biuret’s Agent, egg white, chicken broth, distilled water, test tubes, test tube rack, CuSO4, wax pencils 

Common foods for testing: 

- gelatin, Lite and regular pancake syrup, instant and ground coffee, honey, olive oil, peanut oil, candy 

Part II

- digital blood pressure monitor 

- finger pulse oximeter

Methods

Part I 

 Testing for the major food groups

- proteins

- fats

- carbohydrates

Part II 

Human Physiology - Cardiovascular Testing and Experimentation

- Blood pressure

- Human pulse 

- Oxygen saturation 

- experiments - effects of behavior and exercise on these measurements 

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Lab 5 - Cell Division and Mitosis

Materials 

- prepared slides (whole mounts) 

- onion 

- slide with “squashed onion” 

- onion root tip 

Method 

Part I - prepared slides (whole mounts) 

Identify interphase and the stages of the mitosis onion root tip

Part II - Make slide from squashed onion preparation 

Identify interphase and the stages of the mitosis on the onion root tip 

- Identify cells in interphase as well as all the stages of mitosis: prophase, metaphase, anaphase, telophase and cytokinesis 

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Lab 3: Diffusion and Osmosis

Materials 

- Ink or charcoal 

- agar, IKI, Methylene Blue 

- Cheap perfume 

- agar plates 

- dialysis tubes

- hot and cold water 

- bags 

- thread 

-timer 

- measure glass wear

Exercises

- Know Brownian movement 

- Know the diffusion of molecules in various states (gases, liquids, and solids) 

- Know the role of membranes in diffusion 

- Know the effects of temperature on diffusion across membranes 

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Lab 4: Plants and Energy

Materials

- Potato

- Sweet Potato 

- Yam 

- Rudebaga

- Apple 

- Pear 

Exercises

Part I - Chromatography - use prepared extracts 

Part II - Observe plant slides 

Elodea & Tradescantia 

Part III - Energy storage in plants - potato preparation and stain  

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Lab 2: The Microscope

Materials

- Compound light microscope 

- Dissecting scope

- Various whole mount slides (letter e, threads, etc.)

- Various wet mount preparations (paramecium, volvox, etc.)

- Slides and coverslips 

- Stains

Exercises 

- Know the parts of the Compound light microscope

- Know the parts of the Dissecting scope 

- Learn how to use the Compound light microscope 

- Learn how to use the Dissecting scope

- Learn how to make wet mount slides 

- Learn how to make stain slides 

- Apply knowledge of the microscope, slide preparation, staining in a forensic crime situation 

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Lab 1: Scientific Method Lab

Materials

- Blind

- Penny for random selection

- Data sheet for data collection

- Computer for statistical analysis

Exercises

- Conduct an experiment on Therapeutic Touch

- Test hypothesis that humans have energy fields and are capable of detecting them