#Gang beasts controls pda

[mainsdesign]

Gang beasts controls pda

NPR1 and NPR2 (nitrogen pathogenicity regulation) are wide-domain regulators of nitrogen metabolism required for pathogenicity and induction of MPG1 (hydrophobin gene) expression during nutrient starvation in Ma. However, there is little information in Ma. Since the important and diverse roles of hydrophobins for some fungi have been reported, how the hydrophobin genes are regulated is our concern. In Tricholoma vaccinum (Agaricales), expression levels of nine hydrophobin genes throughout the life cycle revealed their essential role in aerial mycelium, fruiting body and ectomycorrhiza establishment. filiformis markedly increased at the primordium stage, as shown by Northern blot. reported that hydrophobins SC3 and SC4 are expressed in mounds, fruiting bodies and vegetative hyphae of S. There have only been some studies on the gene transcript level of hydrophobin genes. Hydrophobin FBH1 in Pleurotus ostreatus (Agaricales) affected the mycelial growth rate and primordium formation by RNAi transformants. It was reported that a hydrophobin gene, Hyd9, played an important role in the formation of aerial mycelia and primordia in Flammulina filiformis (Agaricales) by RNAi and overexpression. Though the first characterized hydrophobin was SC3 from mushroom-forming fungus Schizophyllum commune (Agaricales), hydrophobins in mushrooms are far from well studied. It seemed that the function of hydrophobic proteins was species specific. Similar results were reported for Botrytis cinerea (Helotiales) in which none of the hydrophobin mutants showed obvious phenotypic defects. Contrary to these reports, individual deletion of the five hydrophobin genes ( HYD1- 5) in Fusarium verticillioides (Hypocreales) indicated that none were required for virulence in a corn seedling infection assay. In mycoparasite fungi Trichoderma longibrachiatum (Hypocreales), hydrophobins affected hydrophobicity of conidia, disease resistance, pathogenicity and plant growth promotion activity. Hydrophobins were required for conidial hydrophobicity and plant root colonization in the fungal biocontrol agent Clonostachys rosea (Hypocreales). acridum (Hypocreales), specifically activated the humoral and cellular immunity of its own host insects. HYD3, a hydrophobin located on the conidial surface of M. In another entomopathogenic fungus, Metarhizium brunneum (Hypocreales), hydrophobins played roles in conidiation, hydrophobicity, pigmentation and virulence. In the entomopathogenic fungus Beauveria bassiana (Hypocreales), the inactivation of hyd1 resulted in reduced spore hydrophobicity and fungal virulence, but a Δ hyd2 mutant displayed decreased surface hydrophobicity of conidia without effects on virulence. In the rice blast fungus Magnaporthe grisea (Magnaporthales), hydrophobins were necessary for fungal development, infection ability and plant colonization. Hydrophobins have been characterized in some fungi, especially pathogenic fungi of plants and insects. militaris, and provide insights into the developmental regulatory mechanism of mushrooms. These results reveal the diverse roles and regulation of Cmhyd1 in C. Furthermore, CmHYD1 is proved to regulate the transcription of Cmarea at different developmental stages via a positive feedback loop. CmAreA, a transcription factor of nitrogen regulatory, is recruited to the promoter of Cmhyd1 and activates the transcription of Cmhyd1 with coactivator CmOTam using electrophoretic mobility shift assays and transient luciferase expression in tobacco. Cmhyd1 also participates in stress response, including tolerance of mycelia to osmotic and oxidative stresses, and conidia to high or low temperatures. Cmhyd1 plays roles in conidiation and cuticle-bypassing infection by regulating the transcripts of frequency clock protein, Cmfrq, and velvet protein, Cmvosa, as well as primordium formation via the mitogen-activated protein kinase signaling pathway. Here, deletion of Cmhyd1 results in reduction in aerial mycelia, conidiation, hydrophobicity and infection ability, and complete inhibition of pigmentation and primordium differentiation. Cmhyd1, one of the hydrophobin class II members in Cordyceps militaris, has been shown to have a high transcript level during fruiting body development. Hydrophobins are a family of small proteins exclusively secreted by fungi, and play a variety of roles in the life cycle.

[darkdoverpseeker]

🕊 [ MxM / OCs / Discord ] Dark themes and taboo kinks in a slowburn romance!

Hello! So as not to waste your time, I’ll cut to the chase!

I’m a 26 year old queer woman in PST; my writing style is in third person past tense with proper grammar, ranging anywhere from one to several paragraphs in length depending on the scene. While I believe in quality over quantity, I’d also like replies that possess a sufficient amount of substance for me to respond to without it being too overbearing (I’ve had partners in the past whose characters would have continual dialogue that offered no pause for mine to react in a realistic manner, which is no fun). My rule of thumb is to have our characters react to whatever occurred in the previous reply, respond to it, then add something further to keep things going!

My current availability allows for multiple replies most days, so a partner with similar availability would be ideal — I definitely don’t hound for replies though! I understand that sometimes such things as responsibilities or even just a more preferable hobby at a certain moment may take priority. Please just bestow the courtesy of letting me know and I’ll do the same unto you! I’m usually free for OOC chat on the side, which is actually very important to me. In addition to the discussion about our roleplay, I like befriending my partner! It’s hard for me to stay invested without that outside rapport.

The topics and themes I’d like to include in our roleplay consist of angst, drama, unhealthy relationships, power imbalances, emotional manipulation, psychological torment, and physical violence. To be honest, I’m looking to roleplay smut with ongoing character development and plot to supplement it; I prefer writing defiant bottoms disciplined into submission, but I’m open to discussing various dynamics based on the characters we come up with! If it hasn’t already been made apparent, my kinks err heavily on the taboo side of things, just a heads up. Due to such explicit subjects, I require you to be 21+ at the very least!

Kinks:

Coercion / dubious consent / non-consensual

Forced pleasure, pleasure control / denial

Mind break, corruption, fucked silly

Humiliation, degradation, dirty talk

Manhandling, hair pulling, restraints

Drugged, drunk, or unconscious state

Sickness, weakness (natural or artificially induced)

Crying, begging, desperation

Gangbang, sloppy seconds, bukkake

Multiple orgasms, over stimulation, sexual exhaustion

Choking, asphyxiation (by either hand or cock)

Scrapes, scratches, slapping, spanking

Light stomach bulge from cock or cum

Ownership (licking, bite marks, collars, etc.)

Frantic fucking, heat / rut

Intercrural (clothed grinding, thigh fucking, etc.)

Blackmail (photographing, videotaping, etc.)

PDA, suggestive touching, public / risky sex, getting caught

Exhibitionism, voyeurism

Cum (creampie, facials, snowballing, swallowing, etc.)

Piss (omorashi, human toilet)

Vomiting (from either throat fucking, or psychological disturbance)

Torture, to an extent (beating, mutilation / removal of small body parts)

Limits:

Underage, incest, beast

Explicit gore, major character death

Farting, scat, diapers, musk, feet, etc.

Listed below in no particular order are some tropes I’ve been interested in trying — we can flesh them out together so both of our tastes can be incorporated! I’m also very open to hearing your ideas if you believe we share similar tastes! To ensure mutual enjoyment of where the story goes, I like to plot a sort of general roadmap before diving into the roleplay, so if you’re the type of writer who likes to “be surprised” and wing it then I’m probably not a good fit for you.

I’d like to incorporate Stockholm Syndrome in these; the roles I’d like to write are in bold:

Killer x Captive: Y/C is obsessed with M/C; eventually kidnapping him, Y/C gradually corrupts M/C to the point where he begins assisting Y/C with his murders, eventually to the point of enthusiasm.

Gang Boss x Lackey: M/C stumbles upon Y/C’s gang activity; deciding M/C is too pretty to kill, Y/C forces him to join the gang, using him for both work and pleasure until he’s been trained to be a loyal guard dog.

Send me a message if you’re interested, even if it’s been a while since it’s been posted! Tell me a bit about yourself, what kind of roleplay you’d like to pursue, and any triggers or limits you’d like to avoid. If I feel like your message lacks effort and/or enthusiasm I’ll most likely ignore it, but if we hit it off after some plotting we can move to Discord!

Happy roleplay partner hunting!

.

[worseandworser]

lacking

Ship: shizaya

Rating: T

Warnings/tags: omegaverse with a twist, possibly OOC, alpha!Shizuo, sigma!Izaya (lol)

ao3 

Izaya Orihara had no scent. Nothing. Not a whiff of it.

Shizuo was taken aback the moment they met — he heard the sound of clapping, saw crimson eyes matching a red shirt and pitch-black hair, but he couldn't smell anything. He couldn't sense anything. The only feeling that boy inspired was a strange perception that there was something lacking about him. Shizuo could recall the growl forming in the depths of his own chest, the mind-wracking wave of rage tangling around his heart like a vine, and then jumping into a fight that would seemingly have no end. Izaya Orihara had no scent; just a smile. Cunning, knife-sharp lips twisted upwards. He dodged Shizuo's attacks and stood unfazed by the intimidating effect Shizuo's anger brought out in every single person he had met in his life.

Being an alpha implied heightened senses, and being Shizuo Heiwajima implied taking that to a whole new level. He could smell a beta — the soft, monochromatic buzz they exhaled — from across the room. Omegas or alphas — depending on the scent, from across the block. Shinra had once theorized that the range of pheromones Shizuo can perceive could be the cause of his temperamental outbursts, as if he was living in a constant state of emotional overload, or something like that. Shizuo didn't really pay attention, much less understand. Though he did get that Izaya Orihara was not alpha, nor beta, and much less an omega.

Sigma, Shinra had said. He can't release or be affected by pheromones.

That alone had sent Shizuo in another surge of anger.

And after that, he went back home and asked his mother about it.

She frowned at him. "Sigma? Do you know someone like that?" Then, at Shizuo's explanation, she shook her head. "Stay away from him. That kind of person is not to be trusted."

In the beginning, Shizuo agreed with her. Not only that, but he thought it'd be easy. No scent meant he wouldn't be walking around Raijin under the constant awareness of Izaya Orihara and pretending he didn't exist would be the easiest thing. It'd be the first time Shizuo wouldn't have to worry about the pheromonal shadow of a bastard who pissed him off.

But of course, Izaya Orihara had different plans.

* * * *

kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill kill

* * * *

The next morning, the asshole was hiding on the school's roof, arms splayed over the railing and the devilish smile plastered to his face.

"You sent that gang after me," Shizuo accused.

With an almost-perfectly crafted innocent expression, Izaya Orihara tilted his head to the side, exposing his neck, but the wave of pheromones Shizuo expected never came. "I have no idea what you are talking about, Shizu-chan."

Shizuo's hands closed in tight fists. "Don't call me that!"

"Ah, since you're asking so politely," said Izaya. "What would Shizu-chan prefer, then? I'm thinking of 'Beast'." He grinned. "Or maybe Monster."

After that, everything became a blur of red, red, red — and black hair tousled by the wind, and the silver gleam of a knife, and the chirping tone of taunting, and crimson eyes that followed Shizuo's every movement like they could eat him alive. And no scent.

Nothing. Not a whiff of it.

It was disturbing, distressing, disconcerting: how could someone who could not feel others, who could not assess their intentions or states, be so quick on his feet and escape Shizuo's grasp like he was made of butter? How could he dodge Shizuo's strength, surpass his speed, predict his strike before even Shizuo could know himself? How could Izaya Orihara, when he was lacking? How could Izaya Orihara know Shizuo Heiwajima, when Shizuo Heiwajima couldn't smell a whiff of Izaya Orihara himself?

He was always so close, Shizuo's hand reached just right next to the black middle-school jacket, and Izaya slipped away by the width of a hair.

And then he laughed, tilting his head to the side, and the sharp breath Shizuo would take as a reflex brought nothing but the scent of their surroundings.

* * * *

Over the years, Shizuo learned more about sigmas. They were rare, extremely so. Izaya had been the first one in Raijin in years. Perhaps, one of the few in the entirety of Tokyo. And just like Shizuo had noticed on the first day they met, sigmas are lacking — no pheromones, no glands, no receptors. If betas are considered to have mild, flexible scents, then sigmas have nothing to show. No pheromones meant they couldn't communicate normally, they couldn't sense you and your needs and you couldn't sense theirs, so all the books, and pamphlets, and late-night TV shows said the same: therefore they can't be trusted.

"I'm always alone no matter what I do," he groused to Celty, after one of his and Izaya's fights. "I guess Izaya is the same as me. That bastard probably doesn't have anyone he can call a friend."

Must be a lonesome existence, that of a sigma. If Shizuo, with his heightened senses and all, had such a hard time with people, he couldn't imagine what it would be like. To not have the confirmation that he wasn't unwanted — to not have the soft sweetness of Celty's scent, in that specific brand that spoke of companionship.

But yeah, that flea did not deserve an ounce of his sympathy.

He is nothing like you, Celty's PDA said.

Shizuo nodded, with an odd hesitation almost weighting his head immobile.

* * * *

One day, Shizuo had his nose buried in Izaya's neck, right where the glands should be, and Izaya still had no scent.

He smelled of skin, sweat, fancy soap, and shampoo. Coffee. The city's traffic. But all of those were neutral, they were supposed to be background noise to the idiosyncratic experience that Izaya Orihara should be but there was nothing. Not a whiff of it.

"Stop slobbering all over my neck," Izaya snapped. "If you bite me, I'll stab your eyeball out of the socket. But thinking better, with your monster healing, you might as well grow a new one in—"

So Shizuo moved back to his lips. To shut him up, of course.

Shizuo wasn't one to sleep around — or better yet, with his particular strength, he couldn't be one to sleep around — but he had tried before. Two omegas and a beta. With the first two, he had felt intoxicated, out of control, it was like losing his temper except that it was good actually, and both smelled like strawberry cake and sweet vanilla, mouth-watering and mind-blowing. The second was an unlucky drunk night, red-dyed hair and a loud, obnoxious laugh, and rain and saltwater and whatever nostalgia he could get from Raijin's library. They all had scents, strong ones, and Shizuo knew what to do simply by breathing in and following his instincts.

But Izaya — just like everything about him, this was also difficult. When Shizuo breathed in, he was left on edge. The oddity of the lack of pheromones struck him over and over, as it did every time they met, as if Shizuo could never be truly prepared for the lack of instinctual balance, of communication, of that irrational connection he had no idea he needed until he met someone who couldn't create it.

To guide himself, Shizuo had to pay attention to other signs. The hitch of Izaya's breath, the twitch of his hips and thighs, the gasps, the whimpers, the moans. The bossing around — Shizu-chan touch here, right there, yes, faster, harder, don't bite don't bite — the way Izaya clawed at his arms and back and chest and did exactly what he told Shizuo not to and bit down, right on Shizuo's neck, right where it mattered, where it would matter were Izaya anyone but himself. But all they got from the bite was blood, the smell bitter and heavy, sending sparks of heat down Shizuo's spine.

Shizuo growled and Izaya let him. Let him. His body bending to Shizuo without the need for words. As if Izaya could sense what Shizuo wanted, as if he could feel it, and accommodate it like he was made for it.

* * * *

In the aftermath, when they laid down on Izaya's expensive sheets, Shizuo curled an arm around him to pull him close. This time, Izaya didn't slip away, no; he hid his face in Shizuo's neck, nosed the bite he left there, and breathed in deep.

Shizuo's hold tightened.