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Transcriptomics is the branch of molecular biology that focuses on the study of RNA transcripts produced by the genome under specific conditions. It provides insights into gene expression patterns, regulatory mechanisms, and cellular responses at a given time.
Key Techniques in Transcriptomics
RNA Sequencing (RNA-Seq) – A high-throughput method to analyze the complete transcriptome using next-generation sequencing (NGS).
Microarrays – A hybridization-based method that detects specific RNA sequences using complementary probes.
qRT-PCR (Quantitative Reverse Transcription PCR) – Used for precise quantification of specific mRNA levels.
Northern Blotting – A traditional method to detect specific RNA molecules.
Single-cell RNA-Seq (scRNA-Seq) – Studies transcriptomics at a single-cell resolution, helping to understand cellular heterogeneity.
Applications of Transcriptomics
Disease Biomarker Discovery – Identifying gene expression changes in diseases like cancer, diabetes, and neurodegenerative disorders.
Drug Development – Assessing how drugs influence gene expression at the cellular level.
Precision Medicine – Personalized treatment strategies based on an individual's transcriptomic profile.
Systems Biology – Understanding how genes interact in biological networks.
Functional Genomics – Linking transcriptome data with gene function and phenotype.
Challenges in Transcriptomics
Data Complexity – Large datasets require advanced bioinformatics tools for analysis.
RNA Stability – RNA is more prone to degradation than DNA, requiring careful handling.
High Cost – RNA-Seq, especially at single-cell resolution, remains expensive.
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Postdoctoral Research Associate in Functional Genomics Washington University School of Medicine ‘Come join us in @WashUGenetics as a #FunctionalGenomics postdoc’ See the full job description on jobRxiv: https://jobrxiv.org/job/washington-university-school-of-medicine-27778-postdoctoral-research-associate-in-functional-genomics-3/?feed_id=51375 #ScienceJobs #hiring #research @WashUGenetics #ScienceJobs #genomics #genetics #ModelOrganization St Louis #UnitedStatesUS #PostdoctoralFellow
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Impaired Fertility and Stress: In the latest edition of the less stress life podcast: @functional_genomic_fertility @jaclyndowns717 and I discuss the different between impaired fertility and infertility. Oxidative stress and or inflammation is probably the biggest reason women struggle with healthy pregnancies. Click on bio to listen to the entire podcast. DM if you have any questions. https://thetruthaboutadrenalfatigue.com/functional-fertility-and-adrenal/ #impairedfertility #functionalmedicine #functionalgenomics #functionalgenomicnutrition #infertility #inferilitysucks #infertilityawareness #infertilitysupport #inferilitysolutions https://www.instagram.com/p/CU-hNxXAQGS/?utm_medium=tumblr
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CERTIFICATE WORKSHOP in ANTIBODY SEQUENCING | FEE ₹ 350 & US $ 8 | FROM 8:30 PM IST (GMT +5:30) 🤳🤳 Recorded video session will be provided to the participants 🌐🌐👉👉Details & Registration https://www.bdglifesciences.com/courses #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #nextgenerationsequencing #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein #canceromics #omics #omicstechnology #repertoiresequencing #wholegenome #rnaseq #dnaseq #AbSequencing #immunerepseq #antibody https://www.instagram.com/p/CNJrXJRD36p/?igshid=6b4sm05gp1k2
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Nope.. not late for @sidramedicine @sidramedicalandresearchcenter #FunctionalGenomics happening in @stregisdoha . I'm really looking forward to this as it's going to be my 2nd time to attend this symposium with them. Loving the venue. #Day1 #nurse #nurselife #nursegoals #symposium #healthcare #ContinuingProfessionalDevelopment #QCHP #BIDCrepresent (at The St. Regis Doha)
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A genome is the complete set of genetic material (DNA or RNA) within an organism, encoding all the information necessary for growth, development, function, and reproduction. It consists of genes, non-coding regions, regulatory elements, and structural components that determine an organism’s traits. The study of genomes, known as genomics, plays a crucial role in biotechnology, medicine, agriculture, and evolutionary biology.
Key Components of a Genome:
Genes: Segments of DNA that encode functional proteins or RNA molecules.
Regulatory Sequences: Control gene expression and include promoters, enhancers, and silencers.
Introns & Exons: Exons encode proteins, while introns are non-coding regions that are spliced out.
Non-Coding DNA: Includes regulatory elements, transposable elements, and structural components like telomeres.
Mitochondrial & Chloroplast Genome: In eukaryotic cells, these organelles have their own separate genetic material.
Epigenetic Modifications: Chemical changes like DNA methylation and histone modification regulate gene expression.
Repetitive DNA Sequences: Includes satellite DNA, transposons, and tandem repeats, which can influence genome stability.
Types of Genomes:
Prokaryotic Genome: Circular, compact, with fewer non-coding regions (e.g., bacteria, archaea).
Eukaryotic Genome: Larger, linear chromosomes housed in a nucleus, with significant non-coding regions.
Viral Genome: Can be DNA or RNA, single or double-stranded, and highly variable in structure.
Organelle Genome: Found in mitochondria and chloroplasts, inherited maternally in most organisms.
Applications of Genome Research in Biotechnology:
Genome Editing (CRISPR-Cas9): Precision modification of genes for disease treatment and crop improvement.
Genetic Engineering: Creating transgenic organisms with desirable traits.
Personalized Medicine: Using genetic information to tailor treatments for individuals.
Agricultural Biotechnology: Developing disease-resistant and high-yield crops.
Synthetic Biology: Designing and synthesizing artificial genomes for biotechnological applications.
Cancer Genomics: Studying genetic mutations in tumors to develop targeted therapies.
Metagenomics: Analyzing microbial communities in different environments for biotech and medical applications.
Evolutionary Genomics: Understanding the genetic basis of evolution and species diversity.
Forensic Genomics: Identifying individuals and ancestry using DNA sequencing.
Epigenomics: Exploring heritable changes in gene expression without altering DNA sequences.
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Mutagenesis is the process of inducing genetic mutations within an organism's DNA, either naturally or artificially. It plays a crucial role in biotechnology, genetics, and molecular biology, enabling scientists to study gene function, develop disease models, and engineer desirable traits in organisms.
Types of Mutagenesis:
Spontaneous Mutagenesis – Naturally occurring mutations due to DNA replication errors, environmental factors, or transposable elements.
Induced Mutagenesis – Mutations triggered by external agents, categorized as:
Chemical Mutagenesis – Uses chemicals like EMS (ethyl methanesulfonate) or nitrosoguanidine to introduce mutations.
Physical Mutagenesis – Uses radiation (UV, X-rays, gamma rays) to cause DNA damage.
Biological Mutagenesis – Utilizes mobile genetic elements, viruses, or transposons to alter DNA.
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Postdoctoral Research Associate in Functional Genomics Washington University School of Medicine ‘Come join us in @WashUGenetics as a #FunctionalGenomics postdoc’ See the full job description on jobRxiv: https://jobrxiv.org/job/washington-university-school-of-medicine-27778-postdoctoral-research-associate-in-functional-genomics-3/?feed_id=50537 #ScienceJobs #hiring #research @WashUGenetics #ScienceJobs #genomics #genetics #ModelOrganization St Louis #UnitedStatesUS #PostdoctoralFellow
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CERTIFICATE WORKSHOP in ANTIBODY SEQUENCING | FEE ₹ 350 & US $ 8 | FROM 8:30 PM IST (GMT +5:30) 🤳🤳 Recorded video session will be provided to the participants 🌐🌐👉👉Details & Registration https://www.bdglifesciences.com/courses #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #nextgenerationsequencing #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein #canceromics #omics #omicstechnology #repertoiresequencing #wholegenome #rnaseq #dnaseq #AbSequencing #immunerepseq #antibody https://www.instagram.com/p/CNB_zT5js70/?igshid=17hxpmnqoxdvw
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TODAY’S CERTIFICATE WORKSHOP in GENETIC COUNSELLING & ETHICAL ISSUES | FEE ₹ 500 & US $ 10 | FROM 8:30 PM IST (GMT +5:30) 🤳🤳 Recorded video session will be provided to the participants Details & Registration https://www.bdglifesciences.com/courses #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #nextgenerationsequencing #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein #canceromics #omics #omicstechnology #repertoiresequencing #wholegenome #rnaseq #dnaseq #geneticcounseling https://www.instagram.com/p/CM3pkncDd2e/?igshid=g1dty3k0d94e
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CERTIFICATE WORKSHOP in Exome Sequencing Analysis | FEE ₹ 320 & US $ 7 | FROM 8:30 PM IST (GMT +5:30) 🤳🤳 Recorded video session will be provided to the participants 👉 Register from here https://www.townscript.com/e/exome-sequencing-online-workshop 🌐 Check out https://www.bdglifesciences.com/ for other programs like Courses, Workshops & Research Projects #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #NGSDataAnalysis #nextgenerationsequencing #metagenomics #metagenome #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein #repertoiresequencing #wholegenome #rnaseq #dnaseq #exomeseq #exome #exomesequencing https://www.instagram.com/p/CMlpc9LDeNe/?igshid=19l87axofrpez
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CERTIFICATE WORKSHOP in Next Generation Sequencing Analysis | FEE ₹ 250 & US $ 8 | FROM 8:30 PM IST (GMT +5:30) 🤳🤳 Recorded video session will be provided to the participants Register from here https://www.townscript.com/e/ngs-course Check out https://www.bdglifesciences.com/ for other programs like Courses, Workshops & Research Projects #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #NGSDataAnalysis #nextgenerationsequencing #metagenomics #metagenome #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein #canceromics #multiomics #omics #omicstechnology #Absequencing #immunerepseq #repertoiresequencing #wholegenome #rnaseq #dnaseq https://www.instagram.com/p/CMbghr-Dz-k/?igshid=17f2xg0qn07di
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CERTIFICATE WORKSHOP in Ab Sequencing Analysis | FEE ₹ 350 & US $ 8 | FROM 8:30 PM IST (GMT +5:30) 🤳🤳 Recorded video session will be provided to the participants Register from here https://www.townscript.com/e/ab-sequencing-analysis-workshop Check out https://www.bdglifesciences.com/ for other programs like Courses, Workshops & Research Projects #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #NGSDataAnalysis #nextgenerationsequencing #metagenomics #metagenome #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein #canceromics #multiomics #omics #omicstechnology #Absequencing #immunerepseq #repertoiresequencing https://www.instagram.com/p/CMRUiLLD-jD/?igshid=16ftdlqfb05fl
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TODAY'S CERTIFICATE WORKSHOP in CANCER OMICS | FEE ₹ 250 & US $ 5 | FROM 8 PM IST (GMT +5:30) 🤳🤳 Recorded video session will be provided to the participants Register from here https://www.townscript.com/e/cancer-omics-course Check out https://www.bdglifesciences.com/ for other programs like Courses, Workshops & Research Projects #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #NGSDataAnalysis #nextgenerationsequencing #metagenomics #metagenome #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein #canceromics #multiomics #omics #omicstechnology https://www.instagram.com/p/CMHBm3KDGUi/?igshid=1xiwz4iv91ib3
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CERTIFICATE WORKSHOP in TRANSCRIPTOME ANALYSIS | Only in ₹ 320 & US $ 7 | FROM 8 PM IST (GMT +5:30) 🤳🤳Video of the session will be recorded and shared with the registered participants. Register from here https://www.townscript.com/e/transcriptome-analysis-workshop Check out https://www.bdglifesciences.com/ for other programs like Courses, Workshops & Research Projects #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #NGSDataAnalysis #nextgenerationsequencing #metagenomics #metagenome #functionalgenomics #genomeanalysis #transcriptome #transcriptomeanalysis #rna #protein https://www.instagram.com/p/CLtf1BLjPor/?igshid=nhezhracglfp
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CERTIFICATE WORKSHOP in NEXT GENERATION SEQUENCING | Live session | Only in ₹ 250 & US $ 5 🤳🤳 Video of the session will be recorded and shared with the registered participants. Register from here https://www.townscript.com/e/ngs-course Check out https://www.bdglifesciences.com/ for other programs like Courses, Workshops & Research Projects #envisionwithbdg #itsdifferentbybiodiscovery #bdglifesciences #bioinformatics #biotechnology #bioinformaticscompany #10yearsofexcellence #biodiscoverygroup #onlineteaching #onlinelearning #NGSDataAnalysis #nextgenerationsequencing #functionalgenomics #genomeanalysis #rna #rnaseq #WholeGenome #DNAseq https://www.instagram.com/p/CLmgMUujaAX/?igshid=dgejnjn9hdxz
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