#henare x reader

[scribensmulier]

His Melting Gaze

pairing: aaron henare x f!reader

word count: 1825

warnings: cnc, chasing, unprotected sex, creampie, degradation, name-calling ("slut" and "woman"), possessiveness, spanking, hair pulling, biting, praise, some mentions of size difference, (the start of) aftercare

*the scene described here takes place in a healthy and safe relationship where all actions have been discussed and negotiated beforehand*

the title is from a poem by the Greek poet Ibycus, which only exists as a fragment: "Once more Eros, under darkened lids, fixing me with his melting gaze, drives me with every kind of spell into the tangling nets of Kypris [Aphrodite]"

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Work is finally done for the day, so you commence your usual end-of-day routine: sitting in your car, scrolling on your phone a bit before driving home. Before you open anything else, you notice you have a text waiting from your boyfriend. 

>Make sure your doors are locked. Someone could break in.

From anyone else, in any other context, this would be a chilling message. But you and Aaron have been together a long time, and you understand what he is truly saying. He’s letting you know what he had planned for your arrival home, and wants to make sure you’re up for it. If not, you could always text back with your safeword, and the evening would continue as normal. Or, you could respond as you, in fact, choose to:

>I’ll be careful, don’t worry.

All thoughts of sifting through social media had left you upon seeing Aaron’s text. You put your phone away and take a deep grounding breath before heading home. You spend the entire drive feeling warm with anticipation, and by the time you pull into the driveway and park, you nearly run to get yourself inside - though you still take the time to remove your panties from beneath your skirt before leaving the car. 

Once you’ve closed and locked the door behind you, you know Aaron will be waiting, so you don’t bother to turn on any lights. However, you hear and see nobody, and set your bag down with a sigh before wandering through the rooms. But they’re all empty too.

You walk back to the living room, more than a little bit disappointed that he didn’t make it home in time to fulfill his promise. You try to remember what he said his plans for the day were-

The moment you step back into the living room, a large form spins you around and presses your back to the wall. There he is.

“Fuck are you smiling at, woman?”

“I- I’m not,” you stammer, remembering the role you are supposed to be playing. “Why are you in my house?”

“Because you have something I want, and you’re going to give it to me.”

“What is it?”

His eyes narrow. “Everything.” 

You tremble at the menace in his voice. No matter anything else, he’s bigger than you, and quite a lot stronger. One hand at your throat, the other pinning you to the wall, you have no hope of escaping his grip. “I don’t know what you mean.” 

“Yes you do.” Aaron grins wickedly before sliding his hand from your neck down to grope one of your breasts. 

“Stop touching me!” You shove hard against his bulk, though he barely moves.

“You’re mine,” he asserts in a low tone. “I can do whatever I want to you.” 

You try to wriggle out of his grasp, and surprisingly he lets you. Lets you, because he could easily keep you pinned in place if he wanted. But no, for some reason, he lets you free, perhaps just to see what happens. Despite the impulse, you don’t run; you only move a few feet away to put some distance between you two. 

You’re breathing heavy, your heart racing, but you can’t seem to break his gaze. He senses your hesitation and steps forward, and you back up only to find yourself against another wall. Damnit. 

“I told you,” he intones, closing the distance quicker than you expected. “You’re mine. You have nowhere to go.” He reaches out again to grab your neck, but he doesn’t grip tightly. He simply rests his hand there as his thumb traces your jawline. Applying just a bit of pressure, he suddenly presses his lips against yours, wasting no time before slipping his tongue into your mouth. For a moment, you think you should try to keep him out, but your body has other ideas and your own tongue meets his before you realize it’s happening.

Aaron makes a hungry sound into your mouth, the kiss growing more erratic with his desire. His body relaxes against yours, which gives you the opening you need to dart away.

You run off, only realizing too late that you ran down the hallway to your bedroom. Instinctively, this feels like the safest place in your home, but right now it’s also a dead end, leaving you trapped. 

You’re frozen just inside the doorway trying to come up with a plan when Aaron seems to materialize behind you, thick arms wrapping around you and swaddling you close. “There she is… you didn’t really want to get away, did you?”

“Yes I did. Yes I do!” You wrestle against his hold, but there’s no chance of getting free. You struggle to get free, but as he continues speaking, your efforts weaken. 

“No you don’t. You ran into here, where you knew I would find you and catch you. Ran right into a cage… a cage with a bed ready for us…” His breath warmed your ear as he whispered millimeters from your skin. “That’s a big bed for one lovely girl. I wonder what you do in this bed when you’re all alone. Do you touch yourself, thinking of all the people you teased during the day with this tempting body? Do you slide your fingers into yourself at the thought that someone might find you here, in this bed with all that empty space around you, and fuck you until you scream into your pillow, over and over and-”

“Stop!” you shout, trying to elbow his bulk behind you. “Stop torturing me. What do you want?”

He softly bites the nape of your neck before responding. “I told you. I want everything. I want you. And I’m going to have you.” Aaron walks you forward toward the bed, shoving you face-first onto the mattress. “Don’t you dare move.”

Despite the rush you felt, you obeyed his command. You lay on the bed, your feet hanging over the edge closest to him. He moved forward, brushing a large hand up the inside of your thigh. He lifts your skirt like it’s Christmas morning and he’s opening a present, movements heavy with anticipation yet knowing he will love whatever he sees. And he certainly does, when he sees your bare skin before him.

“Oh, you really are naughty, aren’t you? Walking around out there with no panties on like a little slut.”

“Hey!” you object, and in response he smacks you hard on the ass, making you yelp.

“Don’t interrupt me, woman. You’re lying here with your pussy out, dripping onto your bed, while a man touches you. Sounds like a slut to me. Do you know what happens to sluts like that?”

Your muscles tense, but he caresses your thighs and hips, carefully running his fingers across the red skin where he hit you. “No…” you manage to stutter out.

“The same thing that is going to happen to you. You’re going to get fucked, right here, right now. And you’re going to love it.”

“No,” you hoarsely whisper, then clear your throat and try again. “No, I’m not.”

“Really?” Aaron pushes your legs apart before running his index finger across your core. “You’re so fucking wet for me, like the slut you are. You want me to fuck you, and you want it now.” Without warning he slides his finger inside, pumping once or twice before joining it with a second finger. 

You can’t help the moans that escape your lips at the feeling of him inside you, whether or not it was your plan. You have to focus hard to not buck against him, seeking more fullness and friction. 

“That’s right, my lovely,” he whispers. “That’s what you like.” Just as suddenly as he began, he takes his fingers away before roughly shoving down his pants and underwear. If you could see behind you, you would see him already hard, fingertips gathering the precum leaking from his slit and rubbing it all over his crown. 

He pulls you roughly to the edge of the bed so that your feet rest on the ground as you’re bent over the mattress before him. With one hand on your ass, he guides his cock to your entrance, resting there to let you feel his presence before entering you. 

“You feel that, my little slut? I’m going to fuck you now, so hold on.” He pushes slowly into you, not wanting to rush despite his overwhelming lust for your body. You whimper as he fills you, your own desire easing the way. 

Once he is fully buried within you, he wastes no time before beginning a steady rhythm, fucking in and out of you with his hands gripping your hips. The feeling of his thick cock thrusting and grazing every sensitive spot in your body has you moaning into the bedspread, trying not to be loud enough to grab his attention.

Of course, he notices immediately. “That’s my good girl,” he mutters, slowing his movements to pull you up by your hair. Though his act is rough, you welcome the sharp pain as he holds you close, releasing your hair to pin you to him by a hand on your throat, his other hand keeping your lower body in place. 

Aaron begins driving into you again, and the new position is deliciously intense. It feels like he’s hammering against your g-spot with every thrust, and you’re only barely aware of the primal sounds you’re emitting as your pleasure spirals. 

You feel his lips against your neck and shoulder, covering you with brutal kisses and occasional nips while working to bring you both to fervent heights. Your hands grope behind you, seeking physical support as your legs weaken and your orgasm approaches.

“Oh fuck, fuck, fuck…” you begin to mutter, and Aaron understands that you’re going to cum. He doesn’t change his movements at all as you dig your nails into his thigh in an attempt to stay upright as your climax hits. He keeps you in place against him while he fucks you through the peak, only slowing once your whimpers stop and you’re panting for air.

Aaron pushes you forward onto the bed again, this time leaning over and caging you beneath him as he desperately fucks into you. He reaches his own orgasm within moments, biting your shoulder as he releases inside you. As his movements slow and then stop, he kisses across the bite mark he just made, then each of the others in turn, every gesture now full of tenderness and love.

After covering you with what never feels like enough kisses, he pulls out of you and lies on the bed, drawing you into an embrace. He continues his gentle touches while you both come down from the exertion.

“Are you alright, love?” he murmurs, combing his fingers through your hair and massaging your scalp. 

You lean into his touch as you respond. “Mmhmm, more than alright. Thank you.”

[journalmaterialscience-blog]

Preliminary Studies on the Protective Effect of Rosmarinus Officinalis on Astrocytes Introduction Different pro-oxidant compounds in the form of reactive oxygen species (ROS) such as hydrogen peroxide, nitric oxide, superoxide and the highly reactive hydroxyl radicals and reactive nitrogen species (RNS) are naturally generated in biological systems. Its production is counteracted by the intrinsic antioxidant defense, both enzymatic and non-enzymatic, which protects against free radicals and the subsequent cell damage [1]. Oxidative damage occurs as an imbalance between the production of ROS and the ability of intrinsic antioxidant systems, to scavenge these radicals. Oxidation of macromolecules such as proteins, lipids and DNA may lead to cell degeneration and death due to an increase in the release of apoptotic inducing factors [2,3]. Brain is especially sensitive to oxidative stress because of the high proportion of unsaturated fatty acids, the high metabolic rate, the low antioxidants proportion and the slow cellular regeneration. Neurodegenerative diseases such as Alzheimer’s, Parkinson or amyotrophic lateral sclerosis have been found to be directly related to oxidative stress increase, elderly being the main risk factor for the development of these kind of diseases, together with toxic metabolic or infectious processes [4-7]. Rosmarinus officinalis L. (Lamiaceae) is an ever green plant spontaneously growing in the Mediterranean area. Aerial parts of rosemary are rich in polyphenolic compounds endorsed with antioxidant activity [8-12]. In continuation with our research line, R. officinalis methanolic extract was assayed on the human astrocyte glioblastoma, which is known as a useful model for the study of astrocyte functions under both physiological and pathological conditions, with the aim of assessing the mechanism of action of the antioxidant ability. In this study, the antioxidant capacity was first evaluated in the R. officinalis methanolic extracts by the oxygen radical absorbance capacity (ORAC) method [13]. Briefly, sample of Trolox was mixed with fluorescein in a 96-multiwell plate and the AAPH added. AAPH was used to generate peroxyl radicals that oxidize fluorescein, causing a decrease in fluorescence (excitation wavelength 485nm and emission wavelength 528nm) which is measured every 4 seconds for 90 minutes at 37 CºC. Then, the effect of Rosemary methanol extract on cell viability was tested in the MTT assay at different concentrations on the human astrocyte glioblastoma U373. Finally, GSH and GSSG/GSH ratio levels were tested to determine whether rosemary extract may influence on this antioxidant defence activity. Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a water-soluble analogue of vitamin E, was chosen as a positive control in all the assays conducted in this work. Trolox is able to decrease ROS production, to prevent cytotoxicity in human cancer cell lines and to rescue cells from apoptotic death [14,15]. Go to Materials and Methods Plant material and extraction process Aerial parts of R. officinalis spontaneously growing in Spain were harvested during flowering in May, 2004. Samples were identified by the Department of Aromatic and Medicinal Plants Research, National Institute of Agricultural and Food Technology (INIA). A voucher specimen was deposited for internal control at the INIA (Madrid, Spain). Samples were dried in an oven at 35°C, grind down and sieved through a 2 mm mesh, and kept protected from light and moisture until use. 60 mg of each sample was extracted with 20 ml Methanol for one hour, under shaking. The suspension was then filtered through one filter paper; 10ml Methanol were added to the sample and filter again over the first methanolic extract. The extract was left for overnight to dry and stored at 5ºC and protected from light until use. Reagents Dulbecco´s modified Eagle´s medium (DMEM), RPMI1640 medium, Foetal bovine serum (FBS), PBS, Gentamicin were purchased from Gibco (Invitrogen, Paisley, UK). Dimethyl sulphoxide (DMSO), Hydrogen peroxide solution (30% w/w), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,7-dichloro-dihydrofluorescein di acetate (DCFH-DA), AAPH were obtained from Sigma-Aldrich (St Louis, MO, USA). Cell culture Human astrocytoma U373 MG line was obtained from Cell Culture and Biological Resources Unit at Alcalade Henares University (Madrid, Spain). Cells were grown in a humidified incubator at 5% CO2 and 95% air at 37ºC in Dulbecco’s Modified Eagle’s medium (DMEM) piruvate free,from Invitrogen (Madrid, Spain),, supplemented with 10% fetal bovine serum (FBS) (Biowhitaker) and 50mg/l of each one of the following antibiotics: gentamicin, penicillin and streptomycin. ORAC assay Sample of Trolox was mixed with fluorescein in a 96-multiwell plate and the AAPH added. AAPH was used to generate peroxyl radicals that oxidize fluorescein causing a decrease in fluorescence (excitation wavelength 485nm and emission wavelength 528nm) which is measured every 4 seconds for 90 minutes at 37 °C in a multiwell plate reader (FLUO star OPTIMA fluorimeter, BMG LABTECH). Results calculate the relationship of the areas under the curve between blank and samples and are expressed as micromoles of Trolox equivalents per gram. MTT assay Cell viability (cell growth inhibition) was determined by MTT assay [16] with some modification. Cells were incubated in 96-well plates, at density of 5 x 10⁴ cells/well for 24h, then the cells were treated with different concentrations of the Romero extracts (range from 3.13 to 800 µg/ml) for another 24h. Triton X-100 5% was used as a negative control, finally 2mg/ml MTT was added and the plate were incubated for 1 h at 37 °C, then the form azan crystal formed were dissolved by adding DMSO and the absorbance was measured at 550 nm using Digiscan 340 microplate reader (ASYA Hitech GmbH, Eugendorf, Austria). For all the experiments, every sample was analyzed in triplicate, with four plates for each condition. Intracellular ROS production assay ROS production was evaluated by the DCFH-DA assay [17] with some modification, This assay is based on the oxidation of the non fluorescent compound 2′,7′-dichlorofluorescein (DCFH) into the fluorescent compound dichlorofluorescein (DCF) in presence of ROS. Cells were incubated in 96-well plate for 24h and 50µl of 2ʹʹ, 7ʹ-dichlorofluorescin diacetate (DCFH-DA) at a concentration of 10 µM were added for 30 min at darkness. Then, cells were treated with different concentrations of rosemary extract and the generation of ROS was measured for 2h in a microplate fluorescence reader (FLx800, Bio-Tek Instrumentation) with excitation at 480 nm and emission at 510 nm. Determination of the Glutathione levels The GSH and GSSG levels were determined according to the method of Hissin and Hilf [18]. Determination of GSH was performed by adding 50 μL of the sample to a mixture of150 μL of 0.1 M sodium phosphate buffer (pH 8.0) and 20 μL of o-phthaldehyde (1mg/mL methanol). The determination of GSSG was conducted by mixing 50 μL of the sample and 3 μL of N-ethylmaleimide for 30 min in darkness before adding 150 μL of 0.1 N NaOH (pH 12) and 20 μL of o-phthaldehyde (1mg/mL, methanol). Finally, both preparations were incubated for 15 min at room temperature in darkness, and fluorescence was measured at an emission wavelength of 485 nm and an excitation wavelength of 528 nm with a microplate fluorescence reader (FLx800, Bio-Tek Instrumentation). Statistical analysis Stat graphics Centurion 16.1.15 (XV) was used. One-way analysis of variance (ANOVA) followed to Fishers least significant difference (LSD) test was applied to obtain the differences between samples. p < 0.05 was considered as statistically significant. Go to Results and Discussion Results showed a showed a strong antioxidant activity by the ORAC method, with a value of 3.03±0.15 µmol TE/mg (value is mean ± SD, n=3). The direct effect of Rosemary extraction cell viability (MTT) showed no statistically significant differences on cell survival with respect to the control group (untreated cells) for concentrations between 12.5 and 200µg/mL; the lowest (3.13 and 6.25µg/mL) and the highest (400-800µg/ml) concentrations induced a decrease in cell survival although far away from the levels achieved with the toxic alone (Triton) (Figure 1A). Thus, concentrations ranging from 12.5 to 200µg/mL were chosen for the following assays. Pretreatment of cells with doses of 12.5, 25, 50 and 100 μg/mL of the extract for 24h before H2O2 exposure was able to significantly recover cell viability when compared to the negative control, Triton (Figure 1B). To test the effect of different concentrations on intracellular ROS levels, doses of 12.5, 25, 50 and 100 µg/mL of the extract were added and evaluated by the DCFH-DA assay (Figure 2A). H2O2 as the oxidant insult caused an increase in ROS levels by 117% when compared to control cells. Rosemary extract did not increase ROS concentration, this indicating no cellular stress or oxidative damage which could influence the functional conditions of cells. Pretreatment of the cells with the methanolic extract previous to oxidative insult, ROS levels were also inferior to those achieved by untreated cells, although no statistically significant differences were found (Figure 2B). Therefore, neuronal cells treated with the R. officinalis extract seem to be in a favourable condition to face an oxidative challenge. Then the protective effect of rosemary on GSH and GSSG concentration was determined in cells treated with 1mMH2O2 or 1 mM H2O2 plus noted concentrations of extract or Trolox as a positive control (Table 1). A slight depletion of intracellular GSH levels was observed when 1 mM H2O2 was added for 24 h to astrocytes; co-treatment with 0.5mM Trolox completely prevented the depletion of GSH. Co treatment with different rosemary concentrations partially recovered GSH levels, the strongest effect found with 50 µg/mL rosemary extract. Although the GSH recover was no statistically significant, the ratio GSSG/GSH was closer to untreated cells (0.46 vs 0.41, respectively). The role of reduced Glutathione (GSH) as the main non-enzymatic antioxidant defence is due to the reaction with free radicals and the repair of free radical induced damage through electron-transfer reactions. Moreover, the loss of cellular GSH seems to have an important role in apoptotic signalling [19-21]. Therefore, maintaining GSH concentration above a critical threshold while facing a stressful situation represents a crucial advantage for cell survival. In conclusion, the results obtained in this work support previous data on the antioxidant effect of R. officinalis [10,11]. Rosemary methanolic extract was not toxic on the assayed cell line and exerted moderate antiradical and antioxidant activities by partially recovering GSH levels. These results may contribute to the knowledge of the mechanism effect, althoughfurther experiments are needed to assess and define the molecular mechanism of action involved in such antioxidant effect in order to confirm R. officinalis as a potential therapeutics within those diseases in which oxidative stress plays a crucial role. For more Open access journals please visit our site: Juniper Publishers For more articles please click on: Journal Material Science juniper publishers material science composite materials