Figure 24.7 shows the analysis of a polypeptide hydrolysate by ion-exchange chromatography.
FIGURE 24.7 Analysis of a mixture of amino acids by ion-exchange chromatography using Amberlite IR-120, a sulfonated polystyrene resin. The resin contains phenyl-SO3-Na+ groups. The amino acid mixture is applied to the column at low pH (3.25), conditions under which the acidic amino acids (Asp, Glu) are weakly bound to the resin and the basic amino acids (Lys, His, Arg) are tightly bound. Sodium citrate buffers of two different concentrations and three different values of pH are used to elute the amino acids from the column. Cysteine is determined as cystine, Cy-S-S-Cys, the disulfide of cysteine.
"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.
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The Protein Hydrolysates Market was valued at USD 3.3 billion in 2023, showcases a remarkable growth projection, anticipated to escalate to USD 4.3 billion by 2028, indicating a robust compound annual growth rate (CAGR) of 5.6% from 2023 to 2028 according to a report published by MarketsandMarkets™.
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Tilapia By-products: Characterizing Fish Protein Hydrolysate
Abstract
Waste management has been a significant problem in the fish processing industry due to environmental and public health impacts. Food products can be developed from the by-products of the aquaculture industry. This study extracted and characterized fish protein hydrolysate (FPH) from tilapia by-products (viscera). It was produced by enzymatic and acid hydrolysis. The degree of hydrolysis (DH), protein pattern, solubility, emulsifying, and foaming properties of the FPH were determined. The yield of the fish protein hydrolysate increased with increasing concentration for acid hydrolysis. Decreasing total protein was observed with the use of increasing HCl concentration. The DH ranged from 12.79-13.95%. The molecular weight distribution of fish protein hydrolysate using acid and enzymatic hydrolysis was analyzed by SDS-PAGE. Limited hydrolysis formed larger peptides which led to improved emulsification and foaming properties of the fish protein hydrolysate. Tilapia intestine crude enzyme hydrolysis produced FPH with higher solubility in water than using acid solutions. The optimum concentration for acid hydrolysis to produce FPH with high emulsifying activity index was found to be 4M acid solution. The Foaming stability for both the acid and enzymatic hydrolysis were low ranging from 9.17% 10.83%. Based on their characteristics and quality, fish protein hydrolysate extracted using acid and enzymatic hydrolysis were within the criteria that can be used as a value-added product in nutraceutical supplements such as sources of small peptides and amino acids in dietetic foods. The improved solubility, emulsifying and foaming capacities of tilapia protein hydrolysate warrant its application in formulated food systems.
Introduction
Tilapia are prepared by bleeding, gutting, beheading, filleting, skinning, and trimming before being bought by consumers. The potential use of fish by-products should be considered. Increasing focus on the utilization of fisheries by-products in product development and value addition can be explained through waste management efforts and characterization of the raw materials as a potential food protein source and functional foods. Several food products could be obtained from the wastes of the aquaculture by-products industry.
Fish protein hydrolysates are products of hydrolysis reaction by breaking the peptide bonds in proteins resulting in shorter peptides or amino acids which are easier for animals to absorb. Extraction of proteins from by-products and conversion to high value products, such as bioactive peptides is a very promising alternative. Bioactive peptide production from fish by-products has received growing attention due to their physiological activities as antioxidant and antihypertensive suitable for healthcare and nutraceutical applications (He et al., 2013; Je et al., 2005; Jung et al., 2006).
The considerable volume of tilapia produced in the country, aside from the significant requirement for processing before final sale generates a large amount of solid waste or residues and by-products, which account for up to 70% of the total fish weight. These so-called wastes composed of the head, carcass, bones, skin, fins and viscera of tilapia are traditionally considered of low economic value and are disposed in land-based waste disposal system or at sea. Moreover, a large amount of fish is also being discarded each year due to fish kill and disease outbreaks. If not properly discarded or used, they can be an important environmental contamination source since the release of these organic wastes might significantly change the community structure and biodiversity of the benthic assemblages(Caruso, 2015). It is estimated that 32 million tons of waste are produced from the total fish capture and are not used as food (Kristinsson & Rasco, 2000). One of the important waste reduction strategies for the industry is the recovery of marketable by‐products from fish wastes (Arvanitoyannis & Kassaveti, 2008). The study was conducted to produce and characterize fish protein hydrolysate from tilapia by-products.
Source: Tilapia By-products: Characterizing Fish Protein Hydrolysate | InformativeBD
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Global Milk Protein Hydrolysate Market – Notable Developments, Upcoming Trends & Future Applications 2032
The global Milk Protein Hydrolysate Market is experiencing substantial growth due to increasing awareness of the health benefits associated with it. Milk protein hydrolysate is a specialized form of milk protein, obtained through enzymatic hydrolysis, breaking down large protein molecules into smaller peptides. These peptides are easier to digest and offer a higher nutritional value, making them suitable for infant formulas, sports nutrition, and clinical nutrition applications.
According to the recent FMI report, the sales for milk protein hydrolysate are expected to cross a value of USD 1.6 Bn in 2032, significantly rising at a CAGR of 6.9% in the time period of 2032.
By 2027, the market for milk protein hydrolysate in sports nutrition applications is expected to exceed USD 0.4 Bn. Sports nutrition products are made up of various types of nutritional food in specified amounts that aid in improving performance and sustaining body energy levels.
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Key Takeaways from the Milk Protein Hydrolysate Market Study:
During the projection period, demand for whey protein hydrolysate in the US is expected to increase by approximately 5.1%. Consumers are paying greater attention to protein-rich products in order to maintain an overall health balance, which is fuelling the expansion of the milk protein hydrolysate sector.
According to FMI analysis, by the end of 2027, the poultry animal feed segment is expected to generate over USD 13.6 Bn in sales. Demand for nutritional and protein-rich food for poultry animals has been encouraged by rising demand for chicken meat and high-quality eggs, which is expected to drive segmental expansion in the coming years.
Due to the rising consumer spending on healthcare and the rising frequency of metabolic illnesses across Latin America, the milk protein hydrolysate market in the country is expected to grow at a CAGR of 4.8% by 2032.
The milk protein hydrolysate market in MEA is valued at over USD 28.3 Bn and is expected to increase significantly during the forecast period, owing to rising product acceptance in dietary supplements and clinical nutrition in the region.
Sports drinks, supplements, and foods such as protein powders, protein bars, supplement powder, ready-to-drink protein beverages, and energy bars are examples of sports nutrition products. Bodybuilders and athletes mostly use these items to increase muscular growth, body performance, and overall wellness, which should boost market figures.
The growth of the sports nutrition segment will also be aided by an increase in the number of young and middle-aged persons participating in sports activities. Milk hydrolysates are important in sports nutrition because they provide a rapid release of amino acids into the bloodstream, which helps muscle and mass development.
Also, the increasing awareness about the benefits of protein-based sports nutrition products paired with an active lifestyle is projected to drive sports nutrition-based milk protein hydrolysate market demand.
“Market players are investing in R&D to create innovative products in order to maintain their market position. Manufacturers are also focusing on implementing technological advancement to improve production efficiency.” said a lead analyst at FMI.
Who is winning?
Leading industry players have recently implemented strategic measures to enter into emerging markets, resulting in increased market dynamics. For example, Kerry, a big nutrition firm, completed the acquisition of Biosearch Life, a functional food and pharmaceutical specialist, in July 2021. Kerry made the strategic acquisition in order to broaden its market offers for health-related supplements and diet additives.
Some of the leading companies offering Milk Protein Hydrolysate are
Fonterra Co-operative Group Ltd.
Kerry Ingredients
Meiji Holdings
MILEI, Friesl and Campina DMV B.V.
AMCO Proteins
Nestle S.A.
Morinaga Milk Industry co. Ltd.
Cargill Inc.
Lactalis Group
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Get Valuable Insights into Milk Protein Hydrolysate Market
FMI, in its new offering, provides an unbiased analysis of milk protein hydrolysate presenting historical demand data (2017-2022) and forecast statistics for the period from (2022-2032). The study divulges compelling insights on the demand for milk protein hydrolysate based on form (powder and paste), by product (whey and casein), by technology (acid hydrolysis, enzymatic, and hydrolysis), by application (sports nutrition, clinical nutrition, infant nutrition, and animal feed) across five major regions.
Information Source: https://www.futuremarketinsights.com/reports/milk-protein-hydrolysate-market
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