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alexcabotgf · 1 month
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moaning my way through this one it's so embarrassing
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csajokamotoron · 26 days
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A LiveWire együttműködik a Mitsubishi Electric céggel
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A korábban a Harley-Davidsonhoz tartozó LiveWire márka azévek alatt komoly motorgyártóváfejlődött. Jelenleg 4 különböző karakterisztikájú modell szerepel a kínálatukban, az S2 Mulholland, az S2 Del Mar, az S2 Del Mar LE, és a LiveWire ONE. A LiveWire S2 Del Mar és S2 Del Mar LE motorkerékpárok az elsők az Egyesült Államokban, amelyekbe a Mitsubishi fejlett elektromos hajtástechnológiáját építették be, amely állítólag optimalizálja a teljesítményt - a 0-60 mérföld/órás gyorsulás három másodperc alatt lehetséges. A sebességre, agilitásra és lineáris gyorsulásra épített S2 Del Mar a városi motorozás és azon túl a LiveWire Electric Motorcycles védjegye. L1 és L2 töltéssel, 3,0 másodperces 0-60-as idővel, 194 ft-lb nyomatékkal a Del Mar úgy lett megépítve, hogy minden másodperc számítson. Az egyedi 19 colos kerék- és gumicsomagot és a LiveWire elektromos hajtásláncát használó Del Mar egy teljesítményorientált motorkerékpár, amely dinamikus menetmódokkal rendelkezik, amelyek azonnal alkalmazkodnak az útviszonyokhoz és a motoros igényeihez. A felfüggesztés a SHOWA® Fully Adjustable első villával és a SHOWA® Free Piston Monoshock hátsó lengéscsillapítóval biztosítja a motoros optimális irányíthatóságát és kényelmét, amely teljes körű beállíthatóságot és precíz kezelhetőséget biztosít. https://csajokamotoron.hu/europaban-is-bemutatkozik-a-livewire-one-elektromos-motorkerekpar/ A Mitsubishi Electric US, Inc. és a Semiconductor and Device Division (SDD) együttműködik a LiveWire EV, LLC-vel, hogy fejlett teljesítményű félvezető modulokat szállítson a LiveWire S2 termékcsaládjához. "A tisztán elektromos élmény, amelyet a Mitsubishi Electric segít lehetővé tenni, átalakító erejű, mivel a motorosok számára pozitív kapcsolatot biztosít a körülöttük lévő világgal motorozás közben, hő, rezgés és zaj nélkül" - mondta Vance Strader, a LiveWire műszaki igazgatója. A Mitsubishi Electric J1 sorozatú teljesítmény félvezető moduljai kompakt, könnyű és nagy teljesítményű szilícium alapú modulok. Jelenleg az autóiparban használják őket, és különböző típusú elektromos járművekhez is alkalmasak. "A J1 sorozat jelentősen hozzájárul az S2 Del Mar lenyűgöző teljesítménymutatóihoz, beleértve a 194 ft-lb nyomatékú villanymotort, a 84 lóerős motort, a 2-es szintű töltés 78 perc alatt történő elérését és a 113 mérföldes városi hatótávolságot. Forrás Read the full article
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rich4you · 1 month
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Check out this listing I just added to my Poshmark closet: Vintage Williams, Bros, Inc. HO 1/87 Scale Kit Corben Super-Ace.
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brookstonalmanac · 6 months
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Birthdays 11.1
Beer Birthdays
Ferdinand Rodenbach (1714)
Benjamin Lee, Baronet Guinness (1798)
Petrus Van Roy (1830)
Arthur Edward Guinness, Lord Ardilaun (1840)
Edmund Fitzgerald (1847)
Henry Schupp (1868 or 69)
Five Favorite Birthdays
William Merritt Chase; artist (1849)
Toni Collette; Australian actor (1972)
Larry Flynt; magazine publisher (1942)
Charlie Kaufman; screenwriter (1958)
Edward Said; Palestinian writer (1935)
Famous Birthdays
Rick Allen; rock drummer (1963)
"Whispering" Bill Anderson; songwriter (1937)
Sholem Asch; Polish writer (1880)
Jules Bastien-Lepage; French artist (1848)
Bo Bice; singer and musician (1975)
Edmund Blunden; English author, poet (1896)
Barbara Bosson; actor (1939)
Hermann Broch; Austrian-American author (1886)
Jan Brożek; Polish mathematician, astronomer (1585)
Tim Cook; Apple Inc. CEO (1960)
Stephen Crane; writer (1871)
Jan Davis; astronaut (1953)
Louis Dewis; Belgian-French painter (1872)
Lou Donaldson; saxophonist (1926)
Richard "Kinky" Friedman; rock singer (1944)
Nordahl Grieg; Norwegian poet (1902)
Michael D. Griffin; physicist and engineer (1949)
Sophie B. Hawkins; rock musician (1967)
Ted Hendricks; Green Bay Packers/Colts/Raiders LB (1947)
Shere Hite; writer, researcher (1942)
Eugen Jochum; German conductor (1902)
Mitch Kapor; Lotus & EFF founder (1950)
Roger Kellaway; pianist, composer (1940)
George Kenner; German-American painte (1888)
Anthony Kiedis; rock singer (1962)
James Kirkpatrick; television journalist (1920)
Robert B. Laughlin; physicist (1950)
Lyle Lovett; singer, songwriter (1957)
L.S. Lowry; British artist (1887)
Jenny McCarthy; model, actor (1972)
Ken Miles; English-American race car driver (1918)
Philip Noel-Baker; Canadian politician, activist (1889)
Gary Player; golf player (1935)
Aishwarya Rai; Indian actor (1973)
Grantland Rice; writer (1880)
Barry Sadler; songwriter (1940)
Jim Steinman; rock songwriter (1947)
Rachel Ticotin; actor (1958)
Fernando Valenzuela; Los Angeles Dodgers P (1960)
Marcia Wallace; actor (1942)
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vividracing · 9 months
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New Post has been published on https://www.vividracing.com/blog/top-5-best-mods-for-the-bmw-m2-g87/
Top 5 Best Mods for the BMW M2 G87
And just like that, BMW did it again, extremely better this time. This pure combustion M car, rumored to be the last from BMW, is both sporty and luxurious with an attractive interior and features that strike a chord. Just like other models in the M series, the M2 is also a high-performance and capable car, but this coupé is set apart with 453 horsepower and 406 lb-ft of torque. We know that car enthusiasts are always excited to add a personal touch to their rides for aesthetics and to get more performance, so we compiled these top 5 modifications in this blog for owners of the BMW M2 G87. Keep reading to see the upgrades that others are getting, and why we recommend them.
1. EBC Brakes 2pc Fully Floating Front & Rear Brake Disc BMW M2 G87 460bhp 2023+
Fully floating brake discs are great upgrades for high-performance cars like BMW M2 G87 as they are;
durable,
allow for better heat dissipation,
improve performance,
and reduce brake fade.
We recommend you get the EBC Brakes 2pc Fully Floating Front & Rear Brake Disc made specifically for your BMW M2 G87 460bhp 2023+. Available for $1,222.07 each, the 380x36mm (front) and 370x24mm (rear) discs can be bolted directly onto the respective axles of these vehicles, offering impressive braking performance and longevity, particularly when driven hard on road and track.
CLICK HERE to get the 2pc Fully Floating Rear Brake Disc
CLICK HERE to buy the 2pc Fully Floating Front Brake Disc
2. Milltek Center Resonator Back System with GT-115 Burnt Titanium Tips BMW 2 Series G87 M2 Coupe S58 NAS Non-OPF | OPF | GPF Models 2023+
The center resonator is famous for fine-tuning the exhaust note, modifying the sound, reducing noise levels, and influencing the overall exhaust flow dynamics. This is why we recommend this $3,975 center resonator by Milltek Sport. Manufactured from T304L stainless steel, this center resonator is durable and can last within some of the toughest environments, as it is far less susceptible to degradation and discoloration.
3. Eventuri Black Gloss Carbon Intake System V2 BMW G8X / G87 M2
We can’t recommend a carbon fiber intake system enough, especially this $2,995 one from Eventuri.  This system will;
Improve airflow
Reduce pressure loss
Increase the flow rate
Improve performance
Improve sound and throttle response
You should also know that this system is future-proof in its capacity to cater to high-powered builds well in excess of 1000hp. The Eventuri G8X M2/M3/M4 intake system consists of a number of components engineered to perform a specific purpose and fabricated to the highest of standards. Made using 100% pre-preg carbon fiber with no fiberglass, this system causes a performance gain of 13-18hp, 12-16ft-lb (Stock Tune)
4. BMC CKS Glossy Paint Carbon Racing Filter Kit BMW Supercar M2 | M3 | M4 G87/G80/G82
This is another performance upgrade for your BMW M2 G87, for the price of $3,000, you can get this upgrade that will lead to performance gains in terms of acceleration, throttle response, and overall engine power. This kit is made by BMC Air Filters, a brand that has been designing, engineering, developing, and producing in-house complex and performance carbon complete intake kits and systems for the world’s most important and renowned car manufacturers for more than 20 years, so you can trust its quality and performance.
Features:
The cotton gauze BMC uses has larger links, allowing a better airflow, but using several layers of oil-soaked cotton allows it to block any impurity larger than 7 microns.
BMC air filters scored an impressive 97,5% filtration efficiency at the ISO5011 test.
Maximum air permeability and higher air flow passage.
BMC equips some of the most important teams in Formula 1, MotoGP, and WorldSBK.
Kit Includes:
1 Cover
2 Airboxes
2 Air Filters
5. Dinan Stainless Steel Valved Axle-Back Exhaust w/ Black Tips BMW M2 G87 2023-2024
The last upgrade we recommend in this blog is an axle-back exhaust system made by Dinan. This system is available in our shop for $1,999.95, and it is the best upgrade for your M2 because of its design features which will help you achieve;
desired sound volume and tone worthy of the G87 M2
allow freer flow
reduce backpressure
This system’s design features also help limit noise during low-speed driving and produce a more subdued, albeit deeper, and agreeable note. However, when the valves are open during high-load conditions the bulk of the gases bypass the muffler altogether for a near-straight pipe design and maximum aural enjoyment to be achieved. Apart from sound, the system is also immaculate looking and makes for an affordable, perfect addition to your ride.
Features:
Maximum gains of +6 HP and +5 lb-ft of torque
Enjoyably louder, sportier sound
Drone abatement technologies used to reduce cabin resonance
Four, diffuser filling, 4.5-inch (114mm) double-walled, polished stainless steel clamp-on tips with laser engraved Dinan logo
Larger diameter piping for increased flow (76mm vs. 70mm)
6-pounds lighter than the stock axle back (Stock: 44-pounds vs. Dinan: 38-pounds)
Stock exhaust valve actuators are reused, and the operation of the exhaust valve is unchanged so the exhaust tone can be adjusted via the M-drive settings
Computer-controlled exhaust valves are retained and limit noise during low-speed driving and open fully during high-load conditions
100% 304 Stainless Steel construction
Factory-like fitment with included mounting hardware
If you have any questions or you would love to buy products that improve your BMW M2 G87’s performance and aesthetics, please do not hesitate to contact us. You can reach us by phone at 1-480-966-3040 or via email at [email protected].
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wiackcom · 9 months
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The 2023 Fisker Ocean is an all-new electric SUV aimed at competing with Tesla and other EV crossovers. Developed by Henrik Fisker's reborn Fisker Inc., the stylish Ocean combines long range, performance, and unique features into an attractive package. This comprehensive review will cover everything potential buyers need to know about the new Fisker Ocean. We'll examine the EV's design, performance specs, interior technology, real-world range, pricing options, and how it compares with rivals. Read on to see if the 2023 Fisker Ocean has the blend of innovation, practicality and value to make waves in the hot EV marketplace. Overview of Fisker Inc and the Ocean SUV Before diving into the new model, let's recap the Fisker brand's origins and ethos: Founded in 2016 after Henrik Fisker's prior venture filed for bankruptcy in 2013. Headquarters and design facilities based in California. Manufacturing partnered with Magna Steyr in Austria. Emphasis on emotionally-engaging vehicle styling inside and out. Focus exclusively on developing electric vehicles with long range and fast charging. Ocean SUV is the brand's first model to reach full production, with deliveries starting in late 2022. The Ocean enters an increasingly crowded field of electric compact/midsize crossover SUVs aiming for mass appeal. But Fisker believes its combination of design flair, range, charging speed, value and innovative features will attract buyers in this booming segment. Engaging Style Inside and Out One area where Henrik Fisker's talents shine through is the Ocean's exterior design: Distinctive sporty proportions without looking cartoonish or overstyled. Short front and rear overhangs enhance agility and ground clearance. Dynamic creases and body lines that flow naturally over the wheel arches. Sleek nose and tapered roofline contrast with muscular haunches. Large wheels pushed outward and detailed lower trim add visual width. Crisp LED lighting and glasshouse contrast the bodywork nicely. The cabin design is modern and filled with space thanks to the dedicated EV platform: Spacious yet intimate feel aided by a low cowl and thin A-pillars. Fisker's old BMW and Aston Martin pedigree shows in the interior layout. Sensible controls and high-resolution screens are intuitive to use. Premium-feeling materials assemble into a minimalist, uncluttered look. Clever small item storage built into lower dash, center console and doors. Both inside and out, the Ocean's styling feels fresh while avoiding polarizing gimmicks. Power and Performance - Up to 564 HP Fisker offers three powertrain configurations for the Ocean: Base Model - Single rear motor making 275 horsepower and torque of 232 lb-ft. 0-60 mph in 6.9 seconds. Starts at $37,499. Mid-level Ultra - Dual motors with all-wheel drive producing 540 hp and 563 lb-ft torque. 0-60 mph in 3.9 sec. Starts at $49,999. Top Extreme - Same AWD dual motor delivering 564 hp and 543 lb-ft torque. 0-60 mph in 3.6 seconds. Starts at $68,999. So performance ranges from capable base power up to supercar-rivaling acceleration in higher grades. Even base models feel responsive thanks to instant electric torque. Steering has precise road feel and tracks evenly through corners. Ride quality balances comfort and control admirably. Fisker's focus on tuning the Ocean for both long range cruising and backroad excitement shows. This electric SUV plays dual roles well, bringing both efficiency and driving enjoyment. Battery Packs and Driving Range Customers also have two lithium-ion battery pack options: Standard Range - 62 kWh capacity providing estimated 250 miles of range on base FWD models. Extended Range - Larger 80 kWh battery ups range to 340 miles on AWD models. Real world range will vary based on driving style and conditions. But the Ocean's larger battery competes with or beats Tesla, Ford Mustang Mach-E and o
ther EV crossover rivals. This long distance capability satisfies most daily commuting and road trip needs. Fast charging capability is impressive. The Ocean accepts up to 200 kW on DC fast chargers. Replenishing 10-80% charge takes just 30 minutes - on par with pricier luxury EVs. Level 2 charging can fully recharge overnight at home. For electric vehicle owners needing to travel long distances occasionally, the Ocean delivers ample battery capacity. Unique Interior Features and Technology Some innovative tech inside the new Fisker Ocean stands out: Rotating center screen - Can flip from portrait to widescreen landscape modes. California Mode - Opens all windows/sunroof with one button press. Head-up augmented reality display - Projects key data like speed on the windshield. Digital rear-view mirror -Works as a standard mirror or video screen if cargo blocks rear view. Recycled interior materials - Interior incorporates plastic waste from ocean debris. On the practical side, rear seat room is generous and cargo space is ample. Cabin storage for personal devices and other small items is excellent. Optional features like a panoramic glass roof enhance the airy, modern interior design. Top-notch infotainment and connectivity includes wireless Apple CarPlay/Android Auto, over-the-air software updates, multiple USB ports and an available WiFi hotspot. How Does the Fisker Ocean Compare to Rivals? The Ocean slots into an exploding electric crossover SUV segment with entries from new and established automakers. Here is how the Fisker stacks up against key competitors: Tesla Model Y - Benchmark for performance and tech. Ocean matches its acceleration and innovation at a lower price. Ford Mustang Mach-E - Similar long range and exciting driving feel. Ocean edgier and more efficient. Volkswagen ID.4 - VW brand familiarity but less stylish. Ocean quicker and longer range. Hyundai Ioniq 5 - Quirky retro styling inside and out. Ocean more mainstream with comparable EV specs. Kia EV6 - Impressive performance and 800V charging but poorer efficiency. Ocean distinctive styling an advantage. The new Fisker Ocean combines strong EV running gear with competitive pricing in this rapidly growing green SUV space. 2023 Fisker Ocean Pricing and Availability Production is ramping up at Magna Steyr's Austria factory, with initial deliveries beginning in late 2022. The 2023 Fisker Ocean lineup includes: TrimDual Motor AWD?Range (Est.)0-60 MPHStarting PriceSportNo250 mi6.9 sec$37,499UltraYes340 mi3.9 sec$49,999ExtremeYes340 mi3.6 sec$68,999 Pricing is quite competitive given the performance, build quality and standard equipment offered. While not discount bargain EVs, Fisker Ocean models deliver strong value, aligning with the brand's emphasis on value-focused sustainable mobility. Conclusion - A True Tesla Challenger Arrives The new 2023 Fisker Ocean makes a compelling case in the midsize electric crossover class. It blends head-turning styling, ample performance, long range, and unique features into a fresh package. Add in competitive pricing and Fisker has a winning formula. While production ramp-up presents some risk, the Ocean appears primed to make big waves in the hotly contested EV space - just as founder Henrik Fisker envisioned. FAQs Where will the Fisker Ocean be built? The Ocean will be produced at contract manufacturer Magna Steyr's factory in Graz, Austria beginning in late 2022. When can I order a 2023 Fisker Ocean? You can reserve an Ocean now via Fisker's website with a $250 deposit. Initial production is slated for late 2022, with order timing depending on your reservation place in line. How long will the Fisker Ocean's battery warranty last? The Ocean will come with a 10 year/100,000 mile battery warranty that protects against defects and excessive degradation. Does the Fisker
Ocean qualify for the $7,500 federal EV tax credit? Yes, the Ocean will qualify for the full $7,500 federal tax credit until Fisker reaches the 200,000 units sold cap. How is Fisker able to make the Ocean so affordable? Strategic outsourcing of manufacturing, extensive use of virtual engineering prototyping, and securing early parts supplier deals help lower costs. #Wiack #Car #CarInsurance #CarRental #CarPrice #AutoLoans
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sportyconnect · 9 months
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The 2023 Fisker Ocean is an all-new electric SUV aimed at competing with Tesla and other EV crossovers. Developed by Henrik Fisker's reborn Fisker Inc., the stylish Ocean combines long range, performance, and unique features into an attractive package. This comprehensive review will cover everything potential buyers need to know about the new Fisker Ocean. We'll examine the EV's design, performance specs, interior technology, real-world range, pricing options, and how it compares with rivals. Read on to see if the 2023 Fisker Ocean has the blend of innovation, practicality and value to make waves in the hot EV marketplace. Overview of Fisker Inc and the Ocean SUV Before diving into the new model, let's recap the Fisker brand's origins and ethos: Founded in 2016 after Henrik Fisker's prior venture filed for bankruptcy in 2013. Headquarters and design facilities based in California. Manufacturing partnered with Magna Steyr in Austria. Emphasis on emotionally-engaging vehicle styling inside and out. Focus exclusively on developing electric vehicles with long range and fast charging. Ocean SUV is the brand's first model to reach full production, with deliveries starting in late 2022. The Ocean enters an increasingly crowded field of electric compact/midsize crossover SUVs aiming for mass appeal. But Fisker believes its combination of design flair, range, charging speed, value and innovative features will attract buyers in this booming segment. Engaging Style Inside and Out One area where Henrik Fisker's talents shine through is the Ocean's exterior design: Distinctive sporty proportions without looking cartoonish or overstyled. Short front and rear overhangs enhance agility and ground clearance. Dynamic creases and body lines that flow naturally over the wheel arches. Sleek nose and tapered roofline contrast with muscular haunches. Large wheels pushed outward and detailed lower trim add visual width. Crisp LED lighting and glasshouse contrast the bodywork nicely. The cabin design is modern and filled with space thanks to the dedicated EV platform: Spacious yet intimate feel aided by a low cowl and thin A-pillars. Fisker's old BMW and Aston Martin pedigree shows in the interior layout. Sensible controls and high-resolution screens are intuitive to use. Premium-feeling materials assemble into a minimalist, uncluttered look. Clever small item storage built into lower dash, center console and doors. Both inside and out, the Ocean's styling feels fresh while avoiding polarizing gimmicks. Power and Performance - Up to 564 HP Fisker offers three powertrain configurations for the Ocean: Base Model - Single rear motor making 275 horsepower and torque of 232 lb-ft. 0-60 mph in 6.9 seconds. Starts at $37,499. Mid-level Ultra - Dual motors with all-wheel drive producing 540 hp and 563 lb-ft torque. 0-60 mph in 3.9 sec. Starts at $49,999. Top Extreme - Same AWD dual motor delivering 564 hp and 543 lb-ft torque. 0-60 mph in 3.6 seconds. Starts at $68,999. So performance ranges from capable base power up to supercar-rivaling acceleration in higher grades. Even base models feel responsive thanks to instant electric torque. Steering has precise road feel and tracks evenly through corners. Ride quality balances comfort and control admirably. Fisker's focus on tuning the Ocean for both long range cruising and backroad excitement shows. This electric SUV plays dual roles well, bringing both efficiency and driving enjoyment. Battery Packs and Driving Range Customers also have two lithium-ion battery pack options: Standard Range - 62 kWh capacity providing estimated 250 miles of range on base FWD models. Extended Range - Larger 80 kWh battery ups range to 340 miles on AWD models. Real world range will vary based on driving style and conditions. But the Ocean's larger battery competes with or beats Tesla, Ford Mustang Mach-E and o
ther EV crossover rivals. This long distance capability satisfies most daily commuting and road trip needs. Fast charging capability is impressive. The Ocean accepts up to 200 kW on DC fast chargers. Replenishing 10-80% charge takes just 30 minutes - on par with pricier luxury EVs. Level 2 charging can fully recharge overnight at home. For electric vehicle owners needing to travel long distances occasionally, the Ocean delivers ample battery capacity. Unique Interior Features and Technology Some innovative tech inside the new Fisker Ocean stands out: Rotating center screen - Can flip from portrait to widescreen landscape modes. California Mode - Opens all windows/sunroof with one button press. Head-up augmented reality display - Projects key data like speed on the windshield. Digital rear-view mirror -Works as a standard mirror or video screen if cargo blocks rear view. Recycled interior materials - Interior incorporates plastic waste from ocean debris. On the practical side, rear seat room is generous and cargo space is ample. Cabin storage for personal devices and other small items is excellent. Optional features like a panoramic glass roof enhance the airy, modern interior design. Top-notch infotainment and connectivity includes wireless Apple CarPlay/Android Auto, over-the-air software updates, multiple USB ports and an available WiFi hotspot. How Does the Fisker Ocean Compare to Rivals? The Ocean slots into an exploding electric crossover SUV segment with entries from new and established automakers. Here is how the Fisker stacks up against key competitors: Tesla Model Y - Benchmark for performance and tech. Ocean matches its acceleration and innovation at a lower price. Ford Mustang Mach-E - Similar long range and exciting driving feel. Ocean edgier and more efficient. Volkswagen ID.4 - VW brand familiarity but less stylish. Ocean quicker and longer range. Hyundai Ioniq 5 - Quirky retro styling inside and out. Ocean more mainstream with comparable EV specs. Kia EV6 - Impressive performance and 800V charging but poorer efficiency. Ocean distinctive styling an advantage. The new Fisker Ocean combines strong EV running gear with competitive pricing in this rapidly growing green SUV space. 2023 Fisker Ocean Pricing and Availability Production is ramping up at Magna Steyr's Austria factory, with initial deliveries beginning in late 2022. The 2023 Fisker Ocean lineup includes: TrimDual Motor AWD?Range (Est.)0-60 MPHStarting PriceSportNo250 mi6.9 sec$37,499UltraYes340 mi3.9 sec$49,999ExtremeYes340 mi3.6 sec$68,999 Pricing is quite competitive given the performance, build quality and standard equipment offered. While not discount bargain EVs, Fisker Ocean models deliver strong value, aligning with the brand's emphasis on value-focused sustainable mobility. Conclusion - A True Tesla Challenger Arrives The new 2023 Fisker Ocean makes a compelling case in the midsize electric crossover class. It blends head-turning styling, ample performance, long range, and unique features into a fresh package. Add in competitive pricing and Fisker has a winning formula. While production ramp-up presents some risk, the Ocean appears primed to make big waves in the hotly contested EV space - just as founder Henrik Fisker envisioned. FAQs Where will the Fisker Ocean be built? The Ocean will be produced at contract manufacturer Magna Steyr's factory in Graz, Austria beginning in late 2022. When can I order a 2023 Fisker Ocean? You can reserve an Ocean now via Fisker's website with a $250 deposit. Initial production is slated for late 2022, with order timing depending on your reservation place in line. How long will the Fisker Ocean's battery warranty last? The Ocean will come with a 10 year/100,000 mile battery warranty that protects against defects and excessive degradation. Does the Fisker
Ocean qualify for the $7,500 federal EV tax credit? Yes, the Ocean will qualify for the full $7,500 federal tax credit until Fisker reaches the 200,000 units sold cap. How is Fisker able to make the Ocean so affordable? Strategic outsourcing of manufacturing, extensive use of virtual engineering prototyping, and securing early parts supplier deals help lower costs. #Wiack #Car #CarInsurance #CarRental #CarPrice #AutoLoans
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welidot · 10 months
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George Clooney
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This Biography is about one of the best Hollywood Actor of the world George Clooney including his Height, weight,Age & Other Detail… Express info Real Name George Timothy Clooney Nickname Gorgeous George Profession American Actor and Filmmaker Age (as in 2023) 62 Years old Physical Stats & More Info Height in centimeters- 180 cm in meters- 1.80 m in Feet Inches- 5’ 11” Weight in Kilograms- 80 kg in Pounds- 176 lbs Body Measurements - Chest: 44 Inches - Waist: 35 Inches - Biceps: 14 Inches Eye Color Dark Brown Hair Color Greyish Personal Life of George Clooney Date of Birth May 6, 1961 Birth Place Lexington, Kentucky, U.S. Nationality American Hometown Augusta, Kentucky, USA School Blessed Sacrament School in Fort Mitchell, Kentucky, St. Michael's School in Columbus, Ohio, Western Row Elementary School, Mason, Ohio, St. Susanna School Mason, Ohio, Augusta High School, Augusta, Kentucky College Northern Kentucky University, Cincinnati, Ohio, University of Cincinnati, Ohio Educational Qualifications      Undergraduate Debut Television Debut - In 1978 mini-series "Centennial" Film Debut - In 1987 for the film "Return to Horror High" Family Father- Nick Clooney (Journalist) Mother- Nina Bruce Warren (Beauty Queen) Sisters- Adelia Clooney Ethnicity English, German, Irish Religion Agnostic Fan Mail Address George Clooney Smoke House Pictures, Inc. 10866 Wilshire Blvd. Suite 1100 Los Angeles, CA 90024 USA Hobbies Playing Baseball, Playing Basketball, Swimming, Travelling, Writing Favourite Things Of George Clooney Favourite Food Steak Favourite Book Leo Tolstoy’s War and Peace Favourite Actresses Michelle Pfeiffer Favourite Color Black Girls , Affairs and More Of George Clooney Marital Status Married Affairs Kelly Preston, Actress (1987-1989) Talia Balsam, Actress (1989-1993) Elizabeth Daily, Actress (1993) Kimberly Russell, Actress (1995) Karen duffy, Actress (1995) VendelaKirsebom, Model (1996) Celine Balitran, Model (1996-1999) Lucy Liu, Actress (2000) Lisa Snowdon, Television Presenter (2000-2005) Traylor Howard, Actress (2000) Jennifer Siebel, Filmmaker (2002) Mariella Frostrup, Journalist (2002) Krista Allen, Actress (2002-2004) Monika Jakisic, Model (2007) Sarah Larson, Actress (2007-2008) ElisabettaCanalis, Actress (2009-2011) Stacy Keibler, Actress (2011-2013) Amal Alamuddin, Lawyer (2013-present) Wife/Spouse Talia Balsam, TV Actress (1989-1993) Amal Clooney, Lawyer (2014-present) Style Of George Clooney Bikes And Cars Collection Tango 600, Chevrolet Corvette V8 C1 Convertible Harley Davidson Motobike, Piaggio Mp3 250 Scooter Earning Money of George Clooney Net Worth $180 million Read the full article
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bakerjohnny1 · 11 months
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Fine Insulation Equipment for All Round Insulation Now
An insulation blower is a claim to fame machine utilized amid the blown-in insulation process. The blower powers cellulose, shake fleece, or fiberglass insulation into divider holes or attics. The machine comprises of a container (for dumping in sacks of free insulation), motor, blower, and hose.
Blower versus Hand Distribution
Main concern: You should lease a blowing insulation machine and not attempt to scatter it by hand. While it is hypothetically conceivable to shake out insulation by hand into attic joist cavities, conveyance is far simpler and scope better circulated in the event that you utilize a blower. One noteworthy obstruction to hand conveyance is the trouble of physically pulling each sack of insulation into the attic and afterward expelling spent packs for the blow in fiberglass insulation.
Rental Yard versus Renting From Home Improvement Store
Primary concern: Typically, the least expensive insulation blower rates are found at home change stores (like a Home Depot) on a one day or one week premise. On a one month premise, rental yards and home change stores have a tendency to have about a similar rental rates. It is conceivable to lease an insulation blower from rental yards or from home change stores like Lowe's or Home Depot. Given that you have your materials effectively arranged, you ought to have the capacity to blow a whole attic in two or three days and surely inside the traverse of one week. Home change stores will regularly require that you buy the cellulose as a major aspect of the blower rental bundle. Frequently, you can get a decreased rental rate on the blower with buy of the cellulose.
Test Rates and Worst Value Rentals
Primary concern: Rental yards offer the most exceedingly awful rates, with half-day rentals being to a great degree low esteem. Rental yard esteem spikes forcefully on a week by week rental premise, yet at the same time no place moving toward home change store rates. Test going rates for an equivalent insulation blower from a rental yard versus a home change store like Home Depot:
Renting versus Buying a Blower: Typical Brand and Quality
Main concern: Normal rental insulation blower and insulation equipment has a container limit of 4.25 cubic feet or 25 lbs. pushing out 350 lbs of insulation for every hour. Weighing 177 pounds, it will require a truck to transport it to your home.
Top-quality blowers have a substantial limit and colossal speed. For instance, the Heat-Seal 5500, pushes between 82 to 135 sacks for each hour (cellulose; fiberglass).
These temporary worker review blowers are genuine creatures: frequently surpassing 1,500 pounds, with measurements of six feet by four feet. Buy costs from makers like Meyer and Heat-Seal, Inc. run from $13,000 to $16,000.
Not all blowers keep running more than five figures in cost. Intec is one producer that makes more financially savvy insulation blowers- - with bring down limit and volume specs. Intec Force 1 is regularly found as a rental.
The Intec Force/2 has a 50 pound or 8 1/2 cu. ft. limit. This model can push out more than 2,000 pounds of cellulose insulation or 428 pounds of fiberglass insulation every hour.
With wheels, this Intec blower weighs around 300 pounds- - pretty much the correct size for the back of a truck.
While higher limit insulation blowers has gas controlled engines, the Intec demonstrate keeps running off of two 20 amp family unit circuits.
To buy an Intec Force/1 will set you back about $4,100; to buy an Intec Force/2 will cost you about $6,400.
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sbknews · 1 year
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European S2 Del Mar Launch Edition – 100 limited edition bikes
European S2 Del Mar Launch Edition – 100 limited edition bikes – goes on sale 27th April. LiveWire®, the leading electric motorcycle manufacturer, is excited to announce an exclusive colourway for the S2 Del Mar Launch Edition in Europe and the UK, which will be limited to 100 bikes across four markets. For EU & UK LiveWire consumers, reservations for the S2 Del Mar Launch Edition will open at 4:00pm. CET on 27th April 2023, and will require a €100 fee. Only 100 examples of the S2 Del Mar European Launch Edition models will be made, featuring an exclusive finish and wheel design. The graphics and paint—in a unique Himalaya White finish—are applied by hand using a process that takes five days to complete. The Launch Edition of S2 Del Mar in Europe is planned for this September. S2 Del Mar European Launch Edition incl. VAT U.K. – £18,990 France – €20,190 Germany – €19,990 Netherlands – €20,390 S2 Del Mar European Launch Edition reveal dates & venues LiveWire will host a series of reveal events of the S2 Del Mar European Launch Edition at the following locations beginning 26th April in London and concluding on 29th April in Berlin. For further information and to RSVP, please visit livewire.com. London – 26th April 26 @ The Bike Shed Motorcycle Club Paris – 27th April @ Roadstar 92 Amsterdam – a 28th April 28 @ H-D Amsterdam Berlin April – a 29th April @ Deus Ex Machina S2 Del Mar Launch Edition Built for agility, and soulful by design, S2 Del Mar is the new mark of performance electric riding, and beyond. With a city range of 110 miles*, and 184 ft-lb of torque, L1 and L2 charging capabilities, over-the-air updates, a 102 mm (4”) TFT LCD dash, and a targeted weight of 195.5 kg, the Del Mar riding experience is instantly familiar as LiveWire and equally an entirely new ride. Complete product specifications will be published in June. S2 Del Mar performance highlights 195.5 kg weight 184 ft-lb (249.5 Nm) torque 110 mile* city range 75 minutes 20-80% L2 charge time 0-60 mph: 3.1 sec: About LiveWire LiveWire has a dedicated focus on the electric motorcycle sector. LiveWire’s majority shareholder is Harley-Davidson, Inc. LiveWire comes from the lineage of Harley-Davidson and is capitalising on a decade of its learnings in the EV sector. LiveWire plans to develop the technology of the future and invest in the capabilities needed to lead the transformation of motorcycling. For more LiveWire news check out our dedicated page LiveWire News or head to the official Harley-Davidson UK website livewire.com/en-gb Read the full article
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boosterusa · 1 year
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Fast Gas Delivery Near Me: Your Solution to Emergencies
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Running out of fuel, Booster is here to refuel !!!
The biggest cellular energy delivery business Booster was established in San Mateo, California. Booster directly provides traditional and alternative fuels and fuel to fleet cars, reducing carbon emissions, cutting expenses, and increasing access to renewable fuels. It is a folksy, tech-focused, and sustainability-obsessed company. During fleets' off-peak hours of operation, the energy-as-a-service provider purchases gasoline from the terminal and distributes it right to the fleets. Fuel is supplied to a fleet of commercial vehicles by a Booster service technician.
To cater to the requirements of a wide range of fleets, Booster's service model adopts an energy-agnostic strategy, acknowledging that each client's development in the energy revolution will look different. The company offers a range of fuels, such as conventional diesel fuel, renewable diesel, and other environmentally friendly fuels.
Booster presently provides services through a strong distributed power delivery network in hundreds of locations from coast to coast. By reimagining fleet fueling through the use of proprietary technologies and logistics, the company is assisting businesses in saving a mean of $1600 and 587 pounds of CO2 yearly per vehicle. Booster was chosen as one of Inc.'s Best in Company in the Logistics & Transportation category in 2022.
Services offered by Booster
Fleets are met by Booster where they are, both geographically and in their pursuit of sustainability. The mobile fueler has an energy-agnostic strategy, enabling Booster to customize its array of traditional and sustainable alternative options to each client's specific energy transition journey. Diesel fuel, diesel, sustainable energy diesel, diesel exhaust fluid, bio-diesel, synthesized blends, and ethanol blends are just a few of the fuels offered by Booster.  The company has also tested a program for mobile electric charging, which would offer a substitute for the permanent charging infrastructure's financial and grid capacity constraints.
Being a technology-driven business, Booster uses cutting-edge haulage, scheduling, and energy delivery systems to provide its service. The company's purple Smarter Tankers are fitted with the most advanced safety measures, including collision and overturning spill protection, unique routing AI, and IoT enablement.
The business also provides significant data dashboard services, which use information gathered during fuel deliveries to give fleet owners and operators in-depth insights. Fuel consumption, spending, projected cost savings, carbon pollution, and gallons pumped are among the metrics.
Booster has provided services for companies like Imperfect Foods, Ferguson Enterprises, Zum, and UPS.
Advantages of mobile fuel supply for fleets
On-site fleet refueling helps keep drivers concentrated on the job.
Average Saving per vehicle: $1600 annual savings on labor, maintenance, and wear and tear
Productivity increased for 61 HOURS: Recover time spent on fuel trips annually per employee
Each vehicle saves 587 LB of carbon emission helping to create a more Resilient Planet
Fewer auto accidents: Every year, there are around 32,000 occurrences at gas stations.
Limit exposure and contact: More than ten thousand times more filthy than public restroom seats, gas stations are severely polluted.
Boost your financial stability: According to the Secret Service, they recover over 25 card scanners per week, with each one containing information from about 80 cards.
Get rid of fuel theft: Due to employee misuse, 3-5% of gasoline purchases on average have yet to reach the specified fleet vehicle.
How does it make the process worth going for it?
Instead of using high-polluting 18-wheelers or wasting time at the gas station, Booster obtains fuel from the terminus and delivers it straight to your fuel system, fuel cell, vehicles, or equipment. The fuel of the highest grade is obtained directly from the terminus. Delivered in our small tankers to your location. Your drivers can concentrate on being productive.
Integrated Data and Insights for Fleet Optimization
Booster observations
Energy dashboard: It puts service, utilization, spending, and vehicle information at your fingertips.
Live information: Data and insights about integrated fleet optimization are delivered right to your device.
Improve your customer service: You can expand your fleet utilization and increase route capacity with insight-based decisions.
Data integrations for fleets
Integration of fleet management: We collaborate with the biggest fleet management firms, including ARI and Enterprise Holdings.
Integrations with fleet cards: To drive insights across your fleet, we integrate with major telematics suppliers like Geotab, Samsara, and Verizon.
360-degree Fleet Data: By utilizing your telemetry data and other crucial services, we provide a complete 360° overview of your fleet.
Small tanker: Our tankers are created from the ground up. They provide vital data collecting and quick field action.
Energy procedures enabled by technology
AI Routing: Extremely efficient routing using AI saves our customer money. The proprietary AI for routing used by Booster creates multidimensional plans for the best routes.
Fuel monitoring: Booster changes to meet your fleet and servicing needs as they change. We continuously monitor demand, tank deployment, and customer performance.
Service monitoring: Complete openness to our clients. The radical visibility into your service, car, and more are unlocked by our cutting-edge vehicle and driver monitors.
Smart Tanker with Cloud Connectivity
Facilitated by IoT: Our unique car tagging technology enhances vehicle identity and fuels information with fail-safes. Our team can increase efficiency thanks to Onboard IoT's full picture of routes and real-time situational data.
Remote management: Our service specialists can remotely manage tanker operations, improving both safety and productivity throughout the whole delivery process.
Safety and dependability: Our operations are the best in the sector thanks to our technology. The tanker steers clear of problems before our service person needs to take action thanks to learning algorithms & wearable technology.
Conclusion
The gas station does not meet the criteria for sustainable, efficient, or easy fleet fueling. Instead of sending every fleet driver to the petrol station, fleet managers ought to look at mobile fueling because it not only saves time and hassle but also money and reduces emissions. Read further on our website to find out how mobile fuelling can reduce emissions and save you money.
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lifestylesdr · 1 year
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Check out this listing I just added to my Poshmark closet: INC International Concepts Rynn Stud Quilted Crossbody Bag Blue Color.
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webpros · 1 year
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Hill's Science Diet Dry Cat Food, Light Adult, Hairball Control, Light for Healthy Weight & Weight Management, Chicken Recipe, 15.5 lb Bag Price: [price_with_discount] Customer satisfaction rating 4.5 (according to Amazon product Details) Hairballs are a natural occurrence for cats, but if they're more frequent than you'd like, you may need a nutritional solution. Hill's Science Diet Hairball Control Light Adult dry cat food provides nutrition to help prevent the formation of hairballs in cats who would benefit from a lighter, healthier lifestyle. Made with natural ingredients, this cat food contains 13% fewer calories compared to Hill's Science Diet Adult Hairball Control, providing your adult cat with a low-calorie source of high-quality protein. The natural fibers are easy to digest and work to comfortably reduce your adult cat's hairball problems. There's more to love with the new look of Science Diet. Some of our products also have new names, new kibble shapes, and new formulas. It is discontinued by the manufacturer ‏ : ‎ No Product Dimensions: 24 x 16 x 4 inches; 15.5 pounds Product model number ‏ : ‎ 8876 Department ‏ : ‎ Dry cat food First available date ‏ : ‎July 20, 2010 Manufacturer ‏ : ‎ Hill's Pet Nutrition Sales, Inc. ASIN ‏ : ‎ B003WK0D8O Low-calorie kibble with 13% fewer calories compared to Hill's Science Diet Adult Hairball Control High-quality protein to help your adult cat build and maintain lean muscle. Recommended For - Adult cats 1 to 6 years of age prone to hairballs and weight gain. Not recommended for - Kittens and pregnant or lactating cats Made with natural ingredients Made in the USA with global ingredients you can trust. #1 Recommended by Veterinarians #Hills #Science #Diet #Dry #Cat #Food #Light #Adult #Hairball #Control #Light #Healthy #Weight #Weight #Management #Chicken #Recipe #Bag See more related items: Hill's Science Diet Dry Cat Food, Light Adult, Hairball Control, Light for Healthy Weight & Weight Management, Chicken Recipe, 15.5 lb Bag Read More: This site is affiliated with Amazon
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bigbootyfoody · 1 year
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Check out this listing I just added to my Poshmark closet: Hadli Zip Around Crossbody, Created for Macy's.
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petnews2day · 1 year
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Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection
New Post has been published on https://petnews2day.com/pet-news/small-pet-news/ceragenin-csa-13-displays-high-antibacterial-efficiency-in-a-mouse-model-of-urinary-tract-infection/
Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection
Bacterial strains
The research was performed using bioluminescent E. coli Xen 14 strain (PerkinElmer Inc., Waltham, MA, United States), and two clinical strains of E. coli isolated from patients (women, age 27 and 36) hospitalized in Independent Public Province Hospital of Jan Sniadecki in Białystok that were diagnosed with cystisis, before antibiotic therapy. The E. coli strains were isolated from clean midstream urine, and were denoted here as strain S1 (S1) and strain S2 (S2). Details on clinical symptoms, a summary of therapy, and other pertinent data were acquired from the hospital information system and laboratory results.
Evaluated compounds
CSA-13, CSA-44 and CSA-131 were synthesized from cholic acid as previously described66. For the purpose of experiments using the animal model, CSA-13 was conjugated with IRDye 800CW fluorescent probe (CSA-13-IRDye 800CW). For this purpose, ceragenin was mixed with IRDye 800CW NHS ester (Li-COR Biosenses, Lincoln, Nebraska, USA) in 10:1 ratio. The mixture was incubated overnight at 4 ºC allowing bond formation between free –NH2 groups of CSA-13 and -NHS reactive group of the probe.
In vitro antimicrobial activity testing
The minimal inhibitory (MIC) and minimal bactericidal (MBC) concentrations of tested ceragenins (CSA-13, CSA-44, CSA-131) against E. coli Xen14 and two clinical strains of uropathogenic E. coli (S1 and S2) were determined in LB (Luria–Bertani) broth using the microdilution method with agent concentrations ranging from 0.5 to 16 µg/mL. The MIC for a given titration series was the lowest ceragenin concentration yielding no visible growth after overnight culture. For MBC determination, aliquots from each overnight MIC dilution series underwent quantitative plating on agar. The MBC was the lowest ceragenins concentration that yielded a ≥ 99.9% decrease in viable counts. The same procedure was employed for estimation of MICs and MBCs of CSA-13 and CSA-13-IRDye800CW in the present of human urine and blood plasma. Antibacterial activity of ceragenins against E. coli Xen14 was confirmed quantitatively using chemiluminescence intensity measurements (allowing investigation of the metabolic activity of ceragenin-treated bacteria) and colony counting assays67,68. For the purpose of chemiluminescence measurements, E. coli Xen14 colonies were grown to mid-log phase at 37 °C, resuspended in Luria–Bertani broth, and diluted to 109 CFU/mL. Then, 100 μL of bacteria suspension was added to each well containing 10 μg/mL of tested compounds. Chemiluminescence intensity was measured during 1 h using a Varioskan Lux microplate reader (Thermo Fisher Scientific, USA). Colonies forming abilities of treated E. coli cells were explored upon incubation with tested agents at concentrations ranging from 1 to 50 µg/mL. Next, the plates were put on ice, and the suspensions were diluted 10- to 1000-fold in PBS. The CFUs were then determined by plating 10 μL aliquots on LB agar for overnight culture at 37 °C. After being exposed to the tested agent, cell survival was expressed in log CFU.
Generation of reactive oxygen species (ROS)
The fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA, Sigma-Aldrich, USA) was used to estimate the generation of ROS induced by ceragenins69. Bacterial cell suspensions (OD600 of 0.1) were pipetted into 96-well black plates. Then, CSA-13, CSA-44, and CSA-131 were added to each well in concentrations ranging from 0 to 100 μg/mL. Later, 20 μM DFCH-DA in PBS was prepared, mixed and added, followed by incubation for 60 min. At excitation/emission wavelengths of 488/535 nm, fluorescence was measured using the microplate reader Varioskan Lux.
Anti-biofilm activity
An incubation of E. coli Xen14 with different concentrations (2–100 μg/mL) of tested compounds for 24–72 h at 37 °C was performed to determine the ability of ceragenins to prevent biofilm formation. An overnight pathogen culture was diluted to 105 CFU/mL. In 96-well polystyrene plates, bacterial suspensions and analyzed agents were mounted, and a biofilm was allowed to expand. The growth medium containing planktonic bacteria was removed after incubation, and the wells were washed 3× with PBS. Crystal violet, 0.1% (w/v), was used to stain the biofilm. The crystal violet working solution was removed after 15 min, and the plates were thoroughly rinsed. The residual biofilm-staining crystal violet was dissolved in 95% ethanol to assess the mass of biofilm. Spectrophotometry analysis (λ = 580 nm) was used to determine the amount of extracted stain.
Cell culture
Human urinary bladder cancer cells T24 (HTB-4™, ATCC) were maintained in McCoy’s 5A cell culture medium supplemented with 10% fetal bovine serum, 50 U/ml penicillin, and 50 mg/mL streptomycin at 37 °C in a 5% CO2 incubator.
Cytotoxicity assays in bacteria-free and bacteria-containing conditions
To assess the cytotoxicity of tested ceragenins toward human urinary bladder cancer cells, a previously established protocol was employed70,71. Briefly, T24 cells were seeded in a 48-well plate at a density of 15–20 × 103 per well and treated with different concentrations of ceragenins for 24 h. The cells were washed with PBS and incubated in a solution of methylthiazoletetrazole (MTT) at a final concentration of 0.5 mg/mL for 4 h. Measurement of spectrophotometric absorbance at 550 nm was performed using a Varioskan Lux microplate reader. Untreated cells were considered to be 100% viable. Each measurement was repeated at least 3 times. An analogous procedure was employed to investigate the abilities of ceragenins to prevent bacteria-induced cytotoxicity. In this experimental setting, E. coli Xen14 strain, both freshly inoculated or heat-inactivated (by boiling bacterial suspension at 121 ºC for 15 min; efficiency of bacteria killing was confirmed by plating the inactivated suspension on agar plates to confirm the loss of outgrowth ability), were suspended in cell culture medium (with the final inoculum being equivalent to 106 CFU/mL) and cultured with ceragenins at doses ranging from 0 to 10 µg/mL.
Compounds used for the animal model
Among the ceragenins, CSA-13 displayed the most favorable activity against E. coli Xen14 and clinical uropathogenic isolates of bacteria (S1 and S2) and was selected for testing in the animal study. Considering the lack of differences in antimicrobial activity of CSA-13 and CSA-13-IRDye800CW and the technical requirements for following biodistribution analyses, we decided to use fluorescent-labelled CSA-13 as a therapeutic agent for animal groups inoculated with E. coli Xen14 strain (group 5). At the same time, to ensure that conjunction of CSA-13 with fluorescent probe did not affect urinary excretion of CSA-13, animals inoculated with clinical strains of uropathogenic E. coli (S1 and S2, groups 6–9) were treated with unlabeled ceragenin (groups 7, 9). Experimental groups used for animal study are indicated in Table 3.
Table 3 Groups of animals used in animal study.
Induction of urinary tract infection in animals
Nude CBy.Cg-Foxn1<nu>/cmdb female mice (The Jackson Laboratory; Bar Harbor, ME USA), aged 9–12 weeks, were used. Mice body weight was monitored prior to induction of infection, after 8 and 12 h post treatment and before sacrificing. Before inducing UTI, urine samples from animals were obtained and cultured to confirm the lack of any bacteria presence in urine. Collected data showed that there were no bacteria in the urine, indicating that all mice subject to this study were free of bacteria prior to induction of infection. To induce infection, female mice weighing from 20 to 25 g were put on their backs after being anesthetized with 5% isoflurane gas and then an inoculum of bacteria [E. coli Xen14 (n = 5, groups 2–3), or 2 clinical strains of uropathogenic E. coli S1 and S2 (n = 5 per each group, groups 4–7)] in 50 μL phosphate-buffered saline (PBS; 1.2 × 107 CFU) was administrated over 30–45 s directly into the bladder via urethral catheter72. Control mice (groups 1, 2, 4, 6) were injected with 0.1 mL of 0.9% NaCl. Following inoculation, the mouse bladders were drained for 4 h to ensure full E. coli adhesion with membrane receptors of bladder epithelial cells. The bacterial inoculum administered was consistent with previously published studies73. After bacterial challenge, the appropriate induction of urinary infection was confirmed by both culture of collected urine (bacteriuria) and histopathological evaluation of the urinary bladder (tissue edema, exfoliation of transitional epithelial cells, invasion of inflammatory cells in the mucosa, bladder mucosa hyperplasia), as well as changes in blood parameters (WBC, RBC, PLT)—Supplementary Table 1.
Treatment procedure
Female mice, 24 h after bacterial challenge with E. coli Xen14, were divided into 2 groups: (1) UTI-suffering, untreated animals, which were dosed intravenously with 0.1 mL of 0.9% NaCl (n = 2; group 4), and (2) UTI-suffering animals, treated i.v. with CSA-13-IRDye800CW at a dose of 5 µg/g of body weight (n = 5; group 3). As a negative control, (3) healthy animals (not challenged with E. coli suspension) were treated with 0.9% NaCl (n = 5; group 1). Another group of mice, 24 h after bacterial challenge (E. coli clinical strains, S1 and S2), were divided into 2 groups: (1) UTI-suffering, untreated animals, which obtained intravenously 0.1 mL of 0.9% NaCl (2 strains of clinical strains of E. coli, S1 and S2, 5 mice per group; groups 4 and 6), and (2) UTI-suffering animals, treated i.v. with CSA-13 at dosage of 5 µg/g of body weight (2 strains of clinical strains of E. coli, S1 and S2, 5 mice per group; groups 5 and 7). As a negative control, (3) healthy animals (not challenged with E. coli suspension) were treated with 0.9% NaCl (n = 5; group 1).
Assessment of bacterial presence in urine
Urine was collected from the animals of groups 1–7 at the specified times (0, 4, 8, 12 and 24 h after treatment) by gentle compression of the bladder through the abdominal wall. A micropipette was used to extract urine samples (5 µL) from the external urethral meatus. Serial dilutions of urine samples were plated on MacConkey agar plates to determine bacterial counts.
Biodistribution of CSA-13-IRDye800CW
For the purpose of analysis of biodistribution of ceragenin, uninfected animals from groups 8 and 9 were injected intravenously with CSA-13-IRDye800CW (n = 5) or unmodified IRDye800CW dye (n = 5), respectively. Mice were anesthetized with 3% isoflurane gas prior to imaging, and the entire animal was imaged for a period of 2 min using the Pearl®Trilogy small animal imaging system (Li-COR, Lincoln, NB, USA) at different times after treatment (0, 4, 8, and 12 h). Mice were held anesthetized with 1–2% isoflurane during the imaging process. Using the Image Studio program 5.2, total photon emissions from specified regions of interest within each mouse image were quantified. The photon signals in ventral images of each mouse were quantified. Mice were euthanized during the procedure and after the final imaging time point, and organs were harvested and scanned using the 800 nm channel for evaluation of the fluorescent location and intensity of CSA-13IRDye800CW/IRDye800CW. Additionally, a portion of collected urine and feces were placed on a glass slide and scanned to determine the intensity of the signal derived from CSA-13-IRDye800CW (n = 5; group 8) or unmodified IRDye800CW dye (n = 5; group 9), respectively.
Histopathological analysis
Bladder tissue samples were surgically removed upon sacrificing animals from groups 1–3, sliced and embedded in 4% buffered formalin. Subsequently, sections were stained with hematoxylin–eosin and histopathological analysis was performed.
Atomic force microscopy
Increasing number of studies indicate that nanoscale mechanical properties of biological tissues, such as stiffness, might be used as a marker of pathologic state59. For the purpose of nanomechanical properties evaluation, fragments of bladder tissues collected from animals inoculated with E. coli Xen14 strain (groups 1, 2, 3, 8 and 9) were stored in Tissue Storage Solution (MACS Media Bergisch Gladbach Germany) and measured within two hours post-surgery. Control, infected and treated bladder tissues were evaluated using atomic force microscope (NanoWizard 4 BioScience JPK Instruments Bruker, Berlin, Germany) working in the Force Spectroscopy mode. Force indentation curves were collected using a silicon nitride cantilevers with a nominal spring constant of 0.7 N/m and measured spring constant in the range of 0.7–1.0 N/m (as determined using thermal tune method), with a 4.5 μm diameter polystyrene bead attached. The AFM cantilevers were manufactured by Novascan Technologies, Inc. (Boone, USA). Tissues were glued onto a Petri dish and immersed in DMEM for the whole measurements that were performed at room temperature. Up to 15 indentation maps consisting of 8 × 8 points corresponding to a scan area of 10 × 10 µm were collected from multiple random places for each tissue sample. Experiments were repeated using five samples per every group studied. The difference between the cantilever deflection on a rigid Petri dish surface and compliant tissue sample describes the deformation of the tissue under the external load. By plotting the force used for sample deformation against the depth of cantilever`s indentation, force-versus-indentation curves were obtained. To determine the elastic modulus (so-called Young’s modulus) that represents sample stiffness, curves were fitted using Hertz contact model as described elsewhere74. Young’s modulus (YM) values distributions for each group were prepared, and the mean YM values for all healthy and infected tissues and cells along with standard deviations were calculated.
Ethical statement
All methods were performed in accordance with the relevant guidelines and regulations. Clinical strains of E. coli isolated from the patients with urinary tract infections were acquired under the approval of Bioethics Committee at the Jan Kochanowski University in Kielce, Faculty of Medicine and Health Sciences (no. 22/2019). Human urine and blood plasma were collected upon approval of Bioethics Committee at the Jan Kochanowski University in Kielce, Faculty of Medicine and Health Sciences (no. 22/2019) and Bioethics Committee at the Medical University of Bialystok (no. R-I-002/231/2019), respectively. Research involving human research participants have been performed in accordance with the Declaration of Helsinki and informed consent has been obtained from the participants involved. Animal experiments were approved by the Local Ethic Committee in Olsztyn of the University of Warmia and Mazury no 64/2020. Animal study is reported in accordance with ARRIVE guidelines.
Statistical analysis
The data described are results from three independent experiments ± SD. The significance of differences was determined using the two-tailed Student’s t-test. Statistical analyses were performed using OriginPro 2020 (OriginLab Corporation, Northampton, USA). P < 0.05 was considered to be statistically significant.
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brookstonalmanac · 1 year
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Birthdays 11.1
Beer Birthdays
Ferdinand Rodenbach (1714)
Benjamin Lee, Baronet Guinness (1798)
Petrus Van Roy (1830)
Arthur Edward Guinness, Lord Ardilaun (1840)
Edmund Fitzgerald (1847)
Henry Schupp (1868 or 69)
Five Favorite Birthdays
William Merritt Chase; artist (1849)
Toni Collette; Australian actor (1972)
Larry Flynt; magazine publisher (1942)
Charlie Kaufman; screenwriter (1958)
Edward Said; Palestinian writer (1935)
Famous Birthdays
Rick Allen; rock drummer (1963)
"Whispering" Bill Anderson; songwriter (1937)
Sholem Asch; Polish writer (1880)
Jules Bastien-Lepage; French artist (1848)
Bo Bice; singer and musician (1975)
Edmund Blunden; English author, poet (1896)
Barbara Bosson; actor (1939)
Hermann Broch; Austrian-American author (1886)
Jan Brożek; Polish mathematician, astronomer (1585)
Tim Cook; Apple Inc. CEO (1960)
Stephen Crane; writer (1871)
Jan Davis; astronaut (1953)
Louis Dewis; Belgian-French painter (1872)
Lou Donaldson; saxophonist (1926)
Richard "Kinky" Friedman; rock singer (1944)
Nordahl Grieg; Norwegian poet (1902)
Michael D. Griffin; physicist and engineer (1949)
Sophie B. Hawkins; rock musician (1967)
Ted Hendricks; Green Bay Packers/Colts/Raiders LB (1947)
Shere Hite; writer, researcher (1942)
Eugen Jochum; German conductor (1902)
Mitch Kapor; Lotus & EFF founder (1950)
Roger Kellaway; pianist, composer (1940)
George Kenner; German-American painte (1888)
Anthony Kiedis; rock singer (1962)
James Kirkpatrick; television journalist (1920)
Robert B. Laughlin; physicist (1950)
Lyle Lovett; singer, songwriter (1957)
L.S. Lowry; British artist (1887)
Jenny McCarthy; model, actor (1972)
Ken Miles; English-American race car driver (1918)
Philip Noel-Baker; Canadian politician, activist (1889)
Gary Player; golf player (1935)
Aishwarya Rai; Indian actor (1973)
Grantland Rice; writer (1880)
Barry Sadler; songwriter (1940)
Jim Steinman; rock songwriter (1947)
Rachel Ticotin; actor (1958)
Fernando Valenzuela; Los Angeles Dodgers P (1960)
Marcia Wallace; actor (1942)
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