#use of Cuvettes in the field of Science

[luckyqueenwitch]

Best Spectro Photometer Manufacturer in Hyderabad

A spectrophotometer is an analytical instrument designed to measure the amount of light absorbed or transmitted by a substance at specific wavelengths. Operating on the principles of the Beer-Lambert law, it establishes a linear relationship between absorbance and concentration, facilitating precise quantitative analyses. The core components of a spectrophotometer include a light source (such as tungsten or deuterium lamps), a monochromator to isolate desired wavelengths, a sample holder (cuvette), and a detector to measure transmitted light intensity. By comparing the intensity of light before and after it passes through a sample, the instrument calculates absorbance, which can then be used to determine the concentration of analytes in various solutions. Spectrophotometers are indispensable across multiple scientific and industrial domains. In the life sciences, they are routinely employed to quantify nucleic acids and proteins, assess enzyme activities, and monitor cell viability. Environmental scientists utilize spectrophotometry to detect and quantify pollutants in water and air, ensuring compliance with environmental regulations. The pharmaceutical industry relies on these instruments for drug development, quality control, and purity assessments. Additionally, the food and beverage sector uses spectrophotometers to analyze color, detect contaminants, and ensure product consistency. In forensic science, they assist in identifying substances based on their absorbance spectra, aiding in criminal investigations. Material scientists also leverage spectrophotometry to study the optical properties of materials, contributing to advancements in nanotechnology and the development of novel materials. research and industrial applications, driving innovation and ensuring quality across various fields.

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[firstsourcels-blog]

The use of Cuvettes in the field of Science

Cuvette is a container-based device resembling a tube which is small having sections in the shape of a square and the sides are straight having open at one end. It is made up of plastic or glass or quartz which makes the device to look transparent. The device is used in the laboratory for measurements and investigations of spectra produced due to electro magnetic radiation. Cuvette is used to measure transmittance, absorbance and fluorescence by passing ray of light through the sample with in the cuvettes. Cuvettes help in holding the liquid in the spectrophotometer. It helps in measuring the light absorbed in the sample. The material available in the form of a layer inside the walls of each Cuvettes will prevent the light passing through side walls which may cause errors in the measurement.

Description: The apparatus includes set of cuvettes which in turn holds number of cuvettes. The main use of the device is the measurements in a vertical way and used in photometer. Optical reading in the cuvettes will be done by bottom window provided in the cuvette. The apparatus is designed in such as way that some cuvettes set are permanently attached and some have the advantage to be detached when required. The set of cuvettes in the apparatus are framed in a way which contains a protective material as a layer which helps in avoiding hindrances during measurement.

Types: Cuvettes are classified into different types which include plastic, quartz and glass. For the purpose of ultra violet experiments quartz Cuvettes which are reusable are used as glass and plastic absorbs ultraviolet light creating errors. Plastic Cuvettes are less inexpensive and are easy to handle due to light weight.

Benefits: The advantages of the hellma cuvettes include preventing of ray of light entering the side walls of the other cuvettes in order to prevent disturbance during measurement. As optical window is the only material serves as the layer between the cuvettes adjacent to each other. The raw materials used in the Cuvette set is free to measure except in the case of optical window which is attached to the adjacent beams may select the raw materials in half or full. The optical wall in the Cuvettes set is designed in such a way that the layer facing inside may have same or different raw materials which acts as binding reagents.

The mode of analysis conducted each time made it reliable and sensible due to same kind of chemical and raw material used inside the cuvette set. The physical ability to bind reagents and the ability contained by each Cuvette have been increased to larger extent. In case a different raw material is used for optical window in the apparatus then third material will be used as coating to bond the reagents either in the earlier stage or later. The coating is done inside for each set of cuvette.

[lab-instruments-blog]

FirstSource Laboratory provides Hellma Cuvettes for spectroscopic analysis. If you want to know how to use of Cuvettes in laboratories please go through this article.

[talesfrothewateringhole]

Tales from the watering hole

Hello and greetings to my fellow water quality citizen science community and research orientated aficionados! My name is Cathal Flood. I am a post graduate reserarcher in Maynooth University. I am also a member of a local citizen science project in Emyvale, Co. Monaghan in Ireland led by Enda Fields of DKIT and Leo McMahon, a local angler whose father was bailiff of emy lough for many years. Local knowledge I have found so informative and inspiring as I did my thesis. What do we do?? Well we take part in monthly testing of various water quality parameters in the Emyvale catchment and also compare this testing to some taking place in Maynooth University that started there with the DCU water blitz with fourth year biology students in the college and Dr. Gail Maher of the department of Biology last September. Obviously given the strange times we are in the midst of the coronavirus outbreak, it is unfeasable for us to continue our regular testing which had only just expanded into nutrient testing for Nitrates thanks to the help and support of DCUs water Institute with Dr.Fiona Regan in particular...but for now we cannot go rouge with our pipettes and cuvettes to break the law in the name of water quality :D  Stay home and stay safe I encourage you all of course. 

However as a water quality enthusiast it is hard for my mind to switch off water and one day on the farm (I am also a part time farmer) which I still have to attend to as an essential service, an epiphany came to me. What if I test here? I always have a great belief  that farmers are part of the solution of our environmental conflicts today and not the problem as some would point to them as. I decided given by luck I still had access to the citizen science basic water monitoring equipment via my nutrient test kits (still had some left) and phosphate testing equipment from Enda (Hanna low range checker) that I could perform some nutrient tests actually on my own farm looking at the effects of land use activities such as slurry and fertilizer application with best practice on the local river and streams which flow through the small 30 acre farm in Ardagh, Co. Longford.

 There are four stream access points for cattle drinking acess or "watering holes" if you will on this farm. I have them restricted as my father did for minimal access but access nonetheless to local water bodies for obvious animal consumption and welfare reasons. My core thought was if I monitored before, during and after grazing of livestock what would it show? What would I see (testing when I am back on the farm every couple of days) . I think the mini study will be interesing first to me to see effects my farm management is having on my local water body. Secondly important to assess nutrient management planning on the farm and compare to other nutrient management systems in general. And finally to show how farmers can learn about their local catchment issues, become advocates of best practice on farm dealing with their local water source for their livestock and become not only custodians of the fruitful land but also of the fresh water! My first I guess experiment was simple. A before and after nutrient test for Nitrates and phosphates during fertilizer/FYM application (April 17th and 23rd). My second nutrient test or experiment is on recently moved livestock from housing to grazing (May 1st) on a 1.5 acre field with one single limited stream access for water consumption as seen in the picture above to assess physical changes in the water body and nutrient changes of course while they are grazing near there. They will be there with maybe some livestock changes (but same quantity of cattle and their poos) til 15th May. I will test again on Friday 8th May.  You are all welcome to provide thoughts or feedback. I am learning too in this process of how to be a more environmentally aware farmer, citizen scientist as well as researcher. Please follow my tales from the watering hole!

Best regards, Cathal. 

[blog-markjohnson07]

Laboratory Equipment and Disposables Market Trends, Opportunities & Revenue Share Analyzed during 2018-2025

The laboratory equipment and disposables market accounted for $24,286 million in 2017, and is expected to reach $37,872 million by 2025, registering a CAGR of 5.7% from 2018 to 2025.

Laboratory is a setting that is equipped to perform scientific experiments, carry out research, and execute analyses of different scientific materials. To perform these activities, the laboratories use equipment and disposables such as incubators, laminar flow hood, micro manipulation systems, centrifuges, lab air filtration system, scopes, sonicators & homogenizers, autoclaves & sterilizers, spectrophotometers & microarray equipment, and other equipment. The disposables required during clinical testing include pipettes, tips, tubes, cuvettes, dishes, gloves, masks, cell imaging consumables, and cell culture consumables. These are used to perform different tests such as analysis of urine, blood, body tissues, and other body fluids. These are also employed in microbiological and pathological testing.

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The major factors such as technological advancements in the field of laboratory equipment, increase in number of clinical diagnostic procedures, and rise in private & public healthcare investments globally drive the market growth. In addition, favorable insurance policies related to laboratory equipment further supplement the market growth. Furthermore, increase in research related to life science and biotechnology boosts the growth of the laboratory equipment and disposables market. However, high cost of technologically advanced laboratory equipment and dearth of clinical laboratories in some nations hamper the market growth. Moreover, growth in adoption of clinical diagnostic testing and high market potential in emerging economies provide lucrative opportunities for the market growth.

This report segments the laboratory equipment and disposables market on the basis of type and region to provide a detailed assessment of the market. Based on type, the market is divided into equipment and disposables. Equipment is further divided into incubators, laminar flow hood, micro manipulation systems, centrifuges, lab air filtration system, scopes, sonicators & homogenizers, autoclaves & sterilizers, spectrophotometers & microarray equipment, and other equipment. Similarly, disposables segment is further classified into pipettes, tips, tubes, cuvettes, dishes, gloves, masks, cell imaging consumables, and cell culture consumables.

Based on region, the market is analyzed across North America (U.S., Canada, and Mexico), Europe (Germany, France, the UK, Italy, Spain, and rest of Europe), Asia-Pacific (China, Japan, Australia, India, Taiwan, and rest of Asia-Pacific), and LAMEA (Brazil, Turkey, South Africa, Saudi Arabia, and rest of LAMEA).

KEY BENEFITS FOR STAKEHOLDERS

The study provides an in-depth analysis of the market along with the current trends and future estimations to elucidate the imminent investment pockets. • It offers a quantitative analysis from 2017 to 2025, which is expected to enable the stakeholders to capitalize on the prevailing market opportunities. • A comprehensive analysis of all the geographical regions is provided to determine the existing opportunities. • The profiles and growth strategies of the key players are thoroughly analyzed to understand the competitive outlook of the global market.

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KEY MARKET SEGMENTS

By Product • Equipment o Incubators o Laminar Flow Hood o Micro Manipulation Systems o Centrifuges o Lab Air Filtration System o Scopes o Sonicators and Homogenizers o Autoclaves and Sterilizers o Spectrophotometers & Microarray Equipment o Others • Disposables o Pipettes o Tips o Tubes o Cuvettes o Dishes o Gloves o Masks o Cell Imaging Consumables o Cell Culture Consumables

By Region • North America o U.S. o Canada o Mexico • Europe o Germany o France o UK o Italy o Spain o Rest of Europe • Asia-Pacific o India o China o Japan o Australia o South Korea o Taiwan o Rest of Asia-Pacific • LAMEA o Brazil o South Africa o Saudi Arabia o Turkey o Rest of LAMEA

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LIST OF KEY PLAYERS PROFILED IN THE REPORT

Agilent Technologies, Inc. • Bio-Rad Laboratories • Bruker Corp • Danaher Corp • Fujifilm Irvine Scientific • PerkinElmer Inc. • Sartorius AG • Shimadzu Corporation • Thermofisher Scientific Inc. • Waters Corp

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Turning light energy into heat to fight disease

https://ift.tt/zeynTX emerging technology involving particles that absorb light and turn it into localized heat sources shows great promise in several fields, including medicine. This heating must be carefully controlled however, and the ability to monitor temperature increases is crucial. Scientists report a method to measure these temperatures using terahertz radiation. The study involved gold nanorods in water in cuvettes, which were illuminated by a laser focused on a spot within the cuvette. from Latest Science News -- ScienceDaily https://ift.tt/2PXGFQ5 December 17, 2019 at 12:42PM An emerging technology involving particles that absorb light and turn it into localized heat sources shows great promise in several fields, including medicine. This heating must be carefully controlled however, and the ability to monitor temperature increases is crucial. Scientists report a method to measure these temperatures using terahertz radiation. The study involved gold nanorods in water in cuvettes, which were illuminated by a laser focused on a spot within the cuvette. from Blogger https://ift.tt/2PBFfvA

[ruggerorespigo]

Turning light energy into heat to fight disease

An emerging technology involving particles that absorb light and turn it into localized heat sources shows great promise in several fields, including medicine. This heating must be carefully controlled however, and the ability to monitor temperature increases is crucial. Scientists report a method to measure these temperatures using terahertz radiation. The study involved gold nanorods in water in cuvettes, which were illuminated by a laser focused on a spot within the cuvette. Latest Science News -- ScienceDaily https://www.sciencedaily.com/releases/2019/12/191217114234.htm

[gxpadvisor]

New Post has been published on LabAutomations

New Post has been published on https://labautomations.com/lego-lab-automation-kit-wtf/

LEGO Lab Automation Kit! WTF

Elementary and secondary school students who later want to become scientists and engineers often get hands-on inspiration by using off-the-shelf kits to build and program robots. But so far it’s been difficult to create robotic projects to foster interest in the “wet” sciences – biology, chemistry and medicine – so called because experiments in these field often involve fluids.

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Now, Stanford bioengineers have shown how an off-the shelf kit can be modified to create robotic systems capable of transferring precise amounts of fluids between flasks, test tubes and experimental dishes.

By combining the Lego Mindstorms robotics kit with a cheap and easy-to-find plastic syringe, the researchers created a set of liquid-handling robots that approach the performance of the far more costly automation systems found at universities and biotech labs.

“We really want kids to learn by doing,” said Ingmar Riedel-Kruse, assistant professor of bioengineering, who led the team that reports its work in the journal PLoS Biology.

“We show that with a few relatively inexpensive parts, a little training and some imagination, students can create their own liquid-handling robots and then run experiments on it – so they learn about engineering, coding and the wet sciences at the same time,” said Riedel-Kruse, who is also a member of Stanford Bio-X.

Robots meet biology

These robots are designed to pipette fluids from and into cuvettes and multiple-well plates –types of plastic containers commonly used in laboratories. Depending on the specific design, the robot can handle liquid volumes far smaller than one microliter, a droplet about the size of a single coarse grain of salt. Riedel-Kruse believes that these Lego designs might even be useful for specific professional or academic liquid-handling tasks where related robots cost many thousands of dollars.

His overarching idea is to enable students to learn the basics of robotics and the wet sciences in an integrated way. Students learn STEM skills like mechanical engineering, computer programming and collaboration while gaining a deeper appreciation of the value of robots in life sciences experiments.

Riedel-Kruse said he drew inspiration from the so-called constructionist learning theories, which advocate project-based discovery learning where students make tangible objects, connect different ideas and areas of knowledge and thereby construct mental models to understand the world around them. One of the leading theorists in the field was Seymour Papert, whose seminal 1980 book Mindstorms was the inspiration for the Lego Mindstorms sets.

“I saw how students and teachers were already using Lego robotics in and outside school, usually to build and program moving car-type robots, and I was excited by that – and the kids obviously as well,” he says. “But I saw a vacuum for bioengineers like me. I wanted to bring this kind of constructionist, hands-on learning with robots to the life sciences.”

Do it yourself

In their PLoS Biology paper, the team offers step-by-step building plans and several fundamental experiments targeted to elementary, middle and high school students. They also offer experiments that students can conduct using common household consumables like food coloring, yeast or sugar. In one experiment, colored liquids with distinct salt concentrations are layered atop one another to teach about liquid density. Other tests measure whether liquids are acids like vinegar or bases like baking soda, or which sugar concentration is best for yeast. Yet another experiment uses color-sensing light meters to align color-coded cuvettes.

The coding aspect of the robot is elementary, Riedel-Kruse said. A simple programming language allows students to place symbols telling the robot what to do: Start. Turn motor on. Do a loop. And so forth. The robots can be programmed and operated in different ways. In some experiments, students push buttons to actuate individual motors. In other experiments, students preprogram all motor actions to watch their experiments executed automatically.

“It’s kind of easy. Just define a few parameters and the system works,” he said, adding, “These robots can support a range of educational experiments and they provide a bridge between mechanical engineering, programming, life sciences and chemistry. They would be great as part of in-school and afterschool STEM programs.”

STEM-ready

Riedel-Kruse said these activities meet several important goals for promoting multidisciplinary STEM learning as outlined by the Next Generation Science Standards (NGSS) and other national initiatives. He stressed the cross-disciplinary instruction value that integrates robotics, biology, chemistry, programming and hands-on learning in a single project.

The team has co-developed these activities with summer high school students and a science teacher, and then tested them with elementary and middle school students over the course of several weeks of instruction. These robots are now ready for wider dissemination to an open-access community that can expand upon the plans, capabilities and experiments for this new breed of fluid-handling robots, and they might even be suitable to support certain research applications.

“We would love it if more students, do-it-yourself learners, STEM teachers and researchers would embrace this type of work, get excited and then develop additional open-source instructions and lesson plans for others to use,” Riedel-Kruse says.

This article has been republished from materials provided by Stanford University. Note: material may have been edited for length and content. For further information, please contact the cited source.

Credit: TechnologyNetworks