#Global C. diff. Events

[cdifffoundation]

A Global Stroll In the Park To Raise C. diff. Awareness On September 25th

A Global Stroll In the Park To Raise C. diff. Awareness On September 25th

Join Us For a Stroll In the Park on September 25th In-Person, and On-Line To Raise C. diff. Awareness Around the Globe!         September 25th is right around the corner and we are so excited to have this opportunity to walk together either in-person at the following park locations: Sims Park, New Port Richey, FL Milton A. Votee Park, Teaneck, NJ  Brown Street Park Complex, Spring City,…

[siva3155]

300+ TOP SPLUNK Interview Questions and Answers

SPLUNK Interview Questions for freshers and experienced :-

1. What Is Splunk? Splunk is Google for your machine data.It’s a software/Engine which can be used for searching, visualizing, Monitoring, reporting etc of your enterprise data. Splunk takes valuable machine data and turns it into powerful operational intelligence by providing real time insight to your data through charts,alerts,reports etc. 2. What would you use to edit contents of the file in Linux? Describe some of the important commands mode in vi editor? Various editors in Linux file system- vi,jedit, ex line editor or nedit Two important modes are as below – We can press ‘Esc’ to switch from one mode to another. However, we can press ‘i’ to enter insert mode- Command mode Insert mode 3. How do you log in to a remote Unix box using ssh? ssh your_username@host_ip_address 4. How you will uncompressed the file? How to install Splunk/app using the Splunk Enterprise .tgz file tar -zxvf file_name.tar.gz tar xvzf splunk_package_name.tgz -C /opt default directory /opt/splunk 5. what does grep() stand for? how to find difference in two configuration files? General Regular Expression Parser. egrep -w ‘word1|word2’ /path/to/file diff -u File_name1.conf File_name2.conf 6. Talk about Splunk architecture and various stages Data Input Stage: Data Storage Stage: Data Searching Stage: Universal forward > Heavy Forward (Optional) > Indexers > Search head Deployment Server- License master- 7. Types Of Splunk Forwarder? Universal forwarder(UF) -Light weight Splunk instance- can’t parse or index data Heavy forwarder(HF) – full instance of Splunk with advance functionality of parsing & indexing 8. Precedence in Splunk and discuss some of the important conf files When 2 or more stanzas specify a behaviour that effects same item, then precedence is calculated based on stanza ASCI We can use priority key to specify highest/lowest priority etc Important conf files props.conf indexes.conf inputs.conf transforms.conf server.conf 9. What is summary index in Splunk? The Summary index is default summary which is used to store data as a result of scheduled searches over period of time. It helps to efficiently process large volume of data. 10. What are types of field extraction. How to mask a data in either of case Search time field extraction Index time field extraction

SPLUNK Interview Questions 11. What do you mean by roles based access control? It is very crucial to provide only appropriate roles to appropriate team. This will prevent unauthorized access to any app or data for that matter. It is very important that we provide access very meticulously and limit their search capability by providing access to only those indexes which needs to be. 12. What is null queue Null queue is an approach to trim out all the unwanted data. 13. Trouble shooting Splunk errors in splunk See if the process is running – ./splunk status IF running go and check log for any latest errors using below command- tail 20 $SPLUNK_HOME/var/log/log/splunk/splunkd.log Splunk crash also happens because of low disk memory- sheck if tere is any crash*log files Check log,splunkd.log,metrics.log or web*log In order to check any conf file related concerns use btool – ./splunk btool props list –debug >/tmp/props.conf Search for errors and warning by typing- Index=_internal | log_level=error OR log_level=warn* Check for the search directory for recent search at – $SPLUNK_HOME/var/ran/splunk/dispatch Enable debug mode.Splunk software has a debug parameter (–debug) that can be used when starting splunk Check for log file OR use below search query – index=_introspection 14. What are the types of search modes supported in splunk? Fast mode Verbose mode Smart mode 15. What is difference between source & source type Source – Identifies as source of data Source type- in general it refers to data structure of events or format of data Different sources may have same source type Command to restart splunk web server /opt/splunk/bin/splunk start splunkweb 16. How to use btool for splunk conf file approach /opt/splunk/bin/splunk cmd btool input list 17. Create new app from templet /opt/splunk/bin/splunk create app New_App -templet sample_App 18. Rollback your aplunk web configuration bundle to previous version /opt/splunk/bin/splunk rollback cluster-bundle 19. To specify minimum disk usage in splunk ./splunk set minfreemb = 20000 ./splunk restart 20. Command to change splunkweb port to 9000 via CLI ./splunk set web-port 9000 21. How to turn down a peer without affecting any other peer of cluster? ./splunk offline 22. How to show which deployment server in configured to pull data from? ./splunk show deploy-poll 23. CLI to validate bundles ./splunk validate cluster-bundle 24. How to see all the license pool active in our Splunk environment? ./splunk list license 25. Which command is used to the “filtering results” category- explain? “search”, “where”. “Sort” and “rex” 26. What is join command and what are various flavours of join command. Join command is used to combine result of a subsearch with result of a search- One or more fields must be common to each results set Inner join- result of inner joint do not include event with NO MATCH Left/Outer join- It include events in the main search and matching having correct field values ..|join type=inner P_id {} 27. Tell me the syntax of Case command It’s a comparison & conditional function Case (X,”Y”,…) X- Boolean expression that are evaluated from first to last. The function defaults to NULL if non is true ..| eval description=case(statsu==20,”OK”,status==404,”NOT FOUND” 28. Which role can create data model Admin & power user 29. Splunk latest version Welcome to Splunk Enterprise 7.2 – Splunk Documentation 30. Which app ships with splunk enterprise Search & reporting Home App 31. How do we convert unix time into string and string back to unix time format strftime(X,Y) : Unix to string as per format strptime(X,Y) : String to UNIX 32. How do we find total number of host or source type reporting splunk instance. Report should consider host across the cluster |metadata type=hosts index=* | convert ctime(firstTime) | convert ctime(lastTime) |convert ctime(recentTime) 33. What is Splunk? Why Splunk is used for analysing machine data? Splunk is a platform for analysing machine data generated from various data sources such as network, server, IOT and so on. Splunk is used for analysing machine data for following reasons Business Intelligence Operational visibility Proactive monitoring Search and Investigation 34. Who are the competitors of Splunk in the market? Why is Splunk efficient? Biggest competitors of Splunk are as follows Sumo logic ELK Loglogic Splunk is efficient as it comes with many inbuilt features like visualization, analysis, apps, Splunk can also be deployed in cloud through Splunk cloud version. Other platforms requires plug in to get additional features. 35. What are the benefits of getting data using forwarders? Data is load balanced by default Bandwidth throttling Encrypted SSL connection TCP connection 36. What happens if License master is unreachable? License Slave sets 72 hour timer and try to reach License Master, after which search is blocked in specific license slave until Master is reachable. 37. What is the command to get list of configuration files in Splunk? Splunk cmd btool inputs list –debug 38. What is the command to stop and start Splunk service? ./splunk stop ./splunk start 39. What is index bucket? What are all stages of buckets? Indexed data in Splunk is stored in directory called bucket. Each bucket has certain retention period after which data is rolled to next bucket. Various stages of buckets are Hot Warm Cold Frozen Thawed 40. What are important configuration files in Splunk? Props.conf inputs.conf outputs.conf transforms.conf indexes.conf deploymentclient.conf serverclass.conf 41. What is global file precedence in Splunk? System local directory – highest priority App local directory App default directory System default directory – lowest priority 42. What is difference between stats and timechart command? Stats                                                                                                                      Timechart Used to represent statistics data in tabular format                  Used to represent search result in graph Can use multiple fields                                                                   Uses _time as default field in x-axis 43. What is lookup command? Lookup command is used to reference fields from an external csv file that matches fields in your event data. 44. What is the role of Deployment server? Deployment server is a Splunk instance to deploy configuration to other Splunk instances from centralized location. 45. What are the default fields in Splunk? Host Source Sourcetype _time _raw 46. What is Search Factor (SF) and Replication Factor (RF) in Splunk? Search factor determines number of searchable copies of data maintained by index cluster. Dafult search factor is 2.Replication factor is the number of copies of data cluster maintains. Search factor should be always less than or equal to Replication factor. 47. What is the difference between Splunk apps and add-ons? Splunk apps contains built-in configurations, reports and dashboards, Splunk add-ons contains only built-in configurations and not visualization (reports or dashboards) 48. How can you exclude some events from being indexed in Splunk? This can be done by using nullQueue in transforms.conf file. For Example: transforms.conf REGEX = DEST_KEY = queue FOMAT = nullqueue 49. Where does Splunk default configuration file located? It is located under $Splunkhome/etc/system/default 50. Discuss about the sequence in which splunk upgrade can be done in a clustered environment? Upgrade Cluster Master Upgrade Search Head Cluster Upgrade Indexer Cluster Upgrade Standalone Indexers Upgrade Deployment Server 51. How do we sync and deploy configurational files and updates across multiple deployment servers in a large multi layered clustered? On one of the deployment server, use below commands- $cd ~ $./DS_sync.sh $/opt/splunk/bin/splunk reload deploy-server -class ServerClassName 52. What would you use to view contents of a large file? How to copy/remove file? How to look for help on a Linux? tail -10 File1 it would show last 10 rows copy file- cp file_name . Remove file command- rm -rf directory_name Manual/help command – man command_name SPLUNK Questions and Answers Pdf Download Read the full article

[sundrenched-smilez]

All the autumnal asks

lantern - how did you meet your best friend? What were your first impressions of each other?

@wwaywward-vvagabond i think we met bc i didnt know what a gif/pic/video of an anime was + u msged me to tell me!! tbh @ first i was rly intimidated by u bc i thought u were a guy until i like took a better look @ ur blog + then we started talkin and just Gosh ur perfect??? and i love u so much, ur the best and deserve the best, and im glad ur in my life <3 <3 <3 

frost - if you could give some advice to your younger self, what would you say?

brush ur teeth !!!!!!!!1 its important,,, 

cider - a food that you disliked as a child but now enjoy?

cant think of any!! i didnt have much of a childhood

amber - share an unpopular opinion that you may have.

im not too huge on coffee, it makes me anxious bc caffeine 

jack-o-lantern - if you could look like any celebrity, who would you choose?

rebecca sugar mb, shes cute, so is kristen stewart, but i wouldnt wanna look like them, that’d b weird 2 me 

orchard - share one thing that you’d like to happen this autumn.

id love a gf or a date mb !! or just to dance w a girl/nb + kiss someone a bunch c: 

crow - which school subject do you wish you had an aptitude for?

history mb? or science

bonfire - describe your dream house.

lots of cats + my wife and spouse and i share a big bed 2gether + make breakfast and we have a great big garden

cinnamon - if you had to live in a time period different than the present, which would you choose and where?

the time period where obama ran a 3rd term + we have a good president, also one where global warming isn’t happening and were chill and use green energy that’s availalble, also, golfing doesnt exist

cobweb - (if you’ve graduated) do you miss high school?

fuck no

cranberry - what’s one physical feature that you get complimented on?

iiiit’s mostly just my clothes tbh. one time a girl told me my face is pretty tho!!! they were rly cute + like yelled it @ me and it warmed my heart

maize - share the weirdest encounter you’ve had with a stranger on the street.

one bought me ice cream after i walked them to the ice cream shop + back to their hotel, and she told me that just from the few minutes she spent w me that i have a good heart + that im a good peron and will meet lots of good friends, and that made me rly happy

quilt - how do you take your tea (or coffee)?

tea, i take hot w milk and sometimes creamer!! coffee is black or w milk/creamer in the rare event that ill drink it 

my fav tea is thai!!

pumpkin - do you think that humans are inherently good or bad?

i think theyre inherently good + im genuinely disturbed and baffled by ppl who intentionally harm others. like they defs exist, and that doesn’t surprise me, but it’s just unconscionable 2 me + i could never do it 

moonlit - are you a neat or messy person? Is your room / house orderly?

i’m pretty neat!! clutter isn’t good + i like to keep a clean home. that bein said, it can tend to get a lil messy bc ive been a wreck lately but im tryin 2 get better abt that

flannel - have you ever gone on a bad date?

only one!! it was rly awkward + every conversation topic i tried was like one-shot answered and unfun

cocoa - if you could have any type of hair, what colour and cut would you have?

probs like the ocean, lots of blues, greens and a touch of purple, vry vibrant, and i would want it soft and thick!! for cut, defs an undercut, w some bangs or enough to like mess w and have diff styles depending on the day

ghost - is there someone that you miss having in your life?

yeah, theres one person i miss talkin to but can’t seem to ever reach, and that’s upsetting to me, bc i felt like we would’ve been good friends, but i cant hold my hand out forever so i try not 2 think abt it a lot. at the time, i was rly needy and just getting out of abuse, so a disaster, + i can’t even say i blame them for leavin honestly

ty for askin !!!

[cdifffoundation]

The 4th Annual Global C. diff. Awareness 2K Walks Go Virtual on September 11 and 12

The 4th Annual Global C. diff. Awareness 2K Walks Go Virtual on September 11 and 12

The 4th Annual Global C.diff. Awareness 2K Walks Will Now Be VIRTUAL!                    Join Us On………………. Friday, September 11th – UK Dr. Clokie, UK Walk Event Coordinator, will be hosting the VIRTUAL Walk in Leicester on September 11th as the UK is also under strict guidelines to slow the spread of the COVID-19 virus. 

The Leicester VIRTUAL Walk Will Begin at 10:00 a.m. – 11:00 a.m. – UK

VI…

[yes-dal456]

Would You Drink A Probiotic With The Gut Bacteria Of Elite Athletes?

Can sneaker endorsements, cereals, protein powders or electrolyte cocktails get any of us closer to the peak level performance of our favorite athletes? Despite billions in sales, the answer is probably no. But how about an elite athlete’s biology?

With 100 trillion cells in the human body, bacteria outnumber our own human cells 2 to 1, and bacteria in our gut affect all our key organ functions. The microbiome plays a role in our health, development and wellness, including endurance, recovery and mental aptitude.

What if we could tap the gut bacteria of elite athletes to produce customized probiotics ― and what if those probiotics could give recipients access to some of the biological advantages that make those athletes elite?

This is a question George Church, professor of genetics at Harvard Medical School, professor of health sciences and technology at Harvard & MIT, and founding core member of the Wyss Institute for Biologically Inspired Engineering, would like to answer.

Jonathan Scheiman confesses that being a research fellow in Church’s lab was not his dream job. “I wanted to play in the NBA. I played basketball at St. John’s in New York. We won a Big East championship my freshman year, an NIT championship my senior year, but I didn’t make the NBA. My backup plan was a Ph.D. in molecular biology.”

One day he mentioned to his mentor that he’d like to sequence LeBron James’s genome, to which Church responded, “Sounds like a great idea, but why not focus on multiple athletes? Why just one?”

At the Wyss, where translation from lab to real world impact is a core principle, the next step was to brainstorm a feasible product. They soon focused on the gut microbiome. Not only can it modulate fitness by affecting energy-metabolism, neurology, resistance to infection, and cellular inflammation, but it can alter physiological states when transferred from one subject to another.

C. difficile affects around 500,000 people a year, usually when antibiotics they take for surgery wipe out their gut microbiome. Then this opportunistic pathogen colonizes the gut and causes all sorts of inflammatory aspects and ulcerative colitis. We currently can cure C. diff infection with 95 percent efficacy through fecal transplants, transferring a healthy microbiome community of organisms into someone that’s infected with this pathogen.

The Wyss team have identified unique differences in the microbiome between elite athletes and non-athletes, as well as bacteria that change before, during, and after athletic events. Preliminary research conducted on Boston Marathon runners and Olympic Trial rowers ― sequencing their microbiome before, leading up to, and after competition ― has revealed bacteria (bugs) that potentially increase energy metabolism and promote recovery.

Jonathan Scheiman lays out the process from start to finish: “Recruit elite athletes, sequence athlete microbiome, identify candidate probiotics, isolate novel beneficial strains and add to food.”

They are looking to sign up 100 elite athletes across the globe to develop an exclusive bank of bacterial samples and related data. They Wyss team will leverage their expertise in genome sequencing technology and microbiology to continue to identify and isolate promising bacterial species. Ultimately they will purify these novel probiotics and commercialize them as ingredients in ingestable products.

Market context: In 2015, the probiotic market was $58B while sports-based nutraceuticals ― with sales of $115B ― made up the largest share of the global nutraceutical market. Yet probiotic-focused sports products made up less than 1 percent of sports-based nutraceuticals.

For Scheiman, this work involves a shift of perspective. “I come from a molecular oncology background. If you look at biomedicine right now … a lot of times we’re looking for deficiencies and then finding ways to correct those to promote health.”

He acknowledges that such work is absolutely essential, but still asks, “What if we look at extremely beneficial phenotypes or extremely healthy people and fit physiology? I think professional and elite athletes are certainly one form of elite phenotypes. They can run faster. They’re stronger. They can jump higher. They’re mentally tough.”

“We’re trying to understand what makes these elite athletes unique from a molecular standpoint, then extract that information and provide it to others to benefit and promote general health and well-being, not only in athletes but maybe one day in the general population.”

When I ask George Church if there is any research that indicates that probiotics, ― including these extracted bacteria from elite athletes ― enhance performance in non-elite athletes, he says, “No, this is brand new. That, I can fairly competently say. It’s not in the literature and peer reviewed journals. I think it will be soon.“

“It’s inevitable that the metabolism of a microorganism can influence the host,” Church asserts. “If you’re willing to make unnatural micro-organisms, you can do almost anything you can do with any other drug delivery method...

“Whether that could be done with naturally occurring organisms taken from elite athletes ― which would be particularly compelling and easy to get buy-in from the community, scientific and public ― that’s I think, a little more speculative. It’s likely enough that we’re pursuing it quite intensely.”

Listen and subscribe on Soundcloud, iTunes, and Google Play.

iTunes: http://ift.tt/2opNPgB

Soundcloud: http://ift.tt/2opMTbT

Wyss release: http://ift.tt/2o2UY9M

-- This feed and its contents are the property of The Huffington Post, and use is subject to our terms. It may be used for personal consumption, but may not be distributed on a website.

from http://ift.tt/2nx7xWF from Blogger http://ift.tt/2oRDpq9

[imreviewblog]

Would You Drink A Probiotic With The Gut Bacteria Of Elite Athletes?

Can sneaker endorsements, cereals, protein powders or electrolyte cocktails get any of us closer to the peak level performance of our favorite athletes? Despite billions in sales, the answer is probably no. But how about an elite athlete’s biology?

With 100 trillion cells in the human body, bacteria outnumber our own human cells 2 to 1, and bacteria in our gut affect all our key organ functions. The microbiome plays a role in our health, development and wellness, including endurance, recovery and mental aptitude.

What if we could tap the gut bacteria of elite athletes to produce customized probiotics ― and what if those probiotics could give recipients access to some of the biological advantages that make those athletes elite?

This is a question George Church, professor of genetics at Harvard Medical School, professor of health sciences and technology at Harvard & MIT, and founding core member of the Wyss Institute for Biologically Inspired Engineering, would like to answer.

Jonathan Scheiman confesses that being a research fellow in Church’s lab was not his dream job. “I wanted to play in the NBA. I played basketball at St. John’s in New York. We won a Big East championship my freshman year, an NIT championship my senior year, but I didn’t make the NBA. My backup plan was a Ph.D. in molecular biology.”

One day he mentioned to his mentor that he’d like to sequence LeBron James’s genome, to which Church responded, “Sounds like a great idea, but why not focus on multiple athletes? Why just one?”

At the Wyss, where translation from lab to real world impact is a core principle, the next step was to brainstorm a feasible product. They soon focused on the gut microbiome. Not only can it modulate fitness by affecting energy-metabolism, neurology, resistance to infection, and cellular inflammation, but it can alter physiological states when transferred from one subject to another.

C. difficile affects around 500,000 people a year, usually when antibiotics they take for surgery wipe out their gut microbiome. Then this opportunistic pathogen colonizes the gut and causes all sorts of inflammatory aspects and ulcerative colitis. We currently can cure C. diff infection with 95 percent efficacy through fecal transplants, transferring a healthy microbiome community of organisms into someone that’s infected with this pathogen.

The Wyss team have identified unique differences in the microbiome between elite athletes and non-athletes, as well as bacteria that change before, during, and after athletic events. Preliminary research conducted on Boston Marathon runners and Olympic Trial rowers ― sequencing their microbiome before, leading up to, and after competition ― has revealed bacteria (bugs) that potentially increase energy metabolism and promote recovery.

Jonathan Scheiman lays out the process from start to finish: “Recruit elite athletes, sequence athlete microbiome, identify candidate probiotics, isolate novel beneficial strains and add to food.”

They are looking to sign up 100 elite athletes across the globe to develop an exclusive bank of bacterial samples and related data. They Wyss team will leverage their expertise in genome sequencing technology and microbiology to continue to identify and isolate promising bacterial species. Ultimately they will purify these novel probiotics and commercialize them as ingredients in ingestable products.

Market context: In 2015, the probiotic market was $58B while sports-based nutraceuticals ― with sales of $115B ― made up the largest share of the global nutraceutical market. Yet probiotic-focused sports products made up less than 1 percent of sports-based nutraceuticals.

For Scheiman, this work involves a shift of perspective. “I come from a molecular oncology background. If you look at biomedicine right now … a lot of times we’re looking for deficiencies and then finding ways to correct those to promote health.”

He acknowledges that such work is absolutely essential, but still asks, “What if we look at extremely beneficial phenotypes or extremely healthy people and fit physiology? I think professional and elite athletes are certainly one form of elite phenotypes. They can run faster. They’re stronger. They can jump higher. They’re mentally tough.”

“We’re trying to understand what makes these elite athletes unique from a molecular standpoint, then extract that information and provide it to others to benefit and promote general health and well-being, not only in athletes but maybe one day in the general population.”

When I ask George Church if there is any research that indicates that probiotics, ― including these extracted bacteria from elite athletes ― enhance performance in non-elite athletes, he says, “No, this is brand new. That, I can fairly competently say. It’s not in the literature and peer reviewed journals. I think it will be soon.“

“It’s inevitable that the metabolism of a microorganism can influence the host,” Church asserts. “If you’re willing to make unnatural micro-organisms, you can do almost anything you can do with any other drug delivery method...

“Whether that could be done with naturally occurring organisms taken from elite athletes ― which would be particularly compelling and easy to get buy-in from the community, scientific and public ― that’s I think, a little more speculative. It’s likely enough that we’re pursuing it quite intensely.”

Listen and subscribe on Soundcloud, iTunes, and Google Play.

iTunes: http://apple.co/2nTHWuG

Soundcloud: http://bit.ly/2nwRhFn

Wyss release: http://bit.ly/2nTKpFl

-- This feed and its contents are the property of The Huffington Post, and use is subject to our terms. It may be used for personal consumption, but may not be distributed on a website.

from Healthy Living - The Huffington Post http://huff.to/2nwUJ2L