#CellBasedAssays

[market-insider]

Exploring the 3D Cell Culture Market: From Research to Real-World Impact

The global 3D cell culture market size is anticipated to reach USD 3.21 billion by 2030 and is anticipated to expand at a CAGR of 11.22% during 2024 to 2030, according to a new report by Grand View Research, Inc. The market is driven by technological advancements in in-vitro testing models, a rising focus on personalized medicine, and supportive government legislation for R&D. Moreover, the increasing prevalence of chronic disorders, and the growing significance of cell therapies in their treatment have created momentum for industry expansion.

Tissue engineering has made significant developments in creating 3D culture models that mimic the in-vivo culture media more precisely than the conventional 2D cell cultures. This resulted in increased utilization of 3D cell culture systems for toxicity testing, drug discovery, and regenerative medicine development. Also, recent product launches from industry players have supported market growth to a significant extent. For instance, in June 2023, Pixelgen Technologies launched its first molecular pixelation kit for 3D spatial study of proteins present on cell surface.

In addition, the development of advanced technologies like microfluidics, bioprinting, and high-content screening systems, has leveraged the capabilities of these models. These technologies allow excellent control over culture conditions, cell organization, and the capability to perform high-efficiency screening, thereby fueling the utilization of 3D culture systems. Moreover, increased collaboration between market players to utilize bioprinting and microfluidics techniques in developing culture models has propelled market growth. For instance, in June 2023, AIM Biotech and MatTek partnered together to offer innovative idenTX and organiX microfluidic 3D tissue culture platforms along with complete drug discovery research services in specific areas of neurobiology, immune-oncology, and vascular biology.

3D cultures can closely replicate the typical microarchitecture and morphology of organs and hence are continuously developed for studies that require in vivo models to analyze the effect of a drug over body tissues and organs. This factor, coupled with the availability of several choices in terms of the material and structure of the scaffold for a variety of in-vitro applications, is anticipated to boost revenue generation for scaffolds. Recent research has explored the use of a broad range of scaffolds, such as graphene scaffolds, nanofibers, natural marine collagen, freeze-casting, and others. In addition, emerging applications of techniques such as lab-on-a-chip in several assay types, including proliferation, stimulation, viability, transport, high content screening, patch clamping, and metabolic activity are anticipated to lead to an increase in demand for advanced and efficient solutions.

However, the lack of consistency in 3D cell model products is one of the major drawbacks that is expected to hinder the growth of the market. Moreover, various factors such as variability in cell culture, standardized challenges, scale & manufacturing issues, and quality control issues might hamper the market growth.

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3D Cell Culture Market Report Highlights

The scaffold-based technology segment dominated the market in 2023 with a revenue share of 48.94% and is attributed to the increasing application of scaffold-based cultures in tissue engineering and regenerative medicine applications

Stem cell research & tissue engineering held the largest share in 2023, whereas the cancer institute segment is expected to witness the fastest growth owing to the rising prevalence of cancer, and the benefits offered by 3D cell cultures in cancer research

In the end-use segment, biotechnology and pharmaceutical companies dominated the market with a revenue share in 2023. The higher revenue growth is attributed to the continuous growth and commercial success of biopharmaceuticals coupled with the expanding portfolio of the major pharmaceutical companies

North America region dominated the global market in 2023 with a revenue share of 38.97%, owing to the presence of advanced healthcare infrastructure, developed economies, the presence of key players, and various strategic initiatives undertaken by them

Gain deeper insights on the market and receive your free copy with TOC now @: 3D Cell Culture Market Report

We have segmented the global 3D cell culture market based on technology, application, end-use, and region.

[elainaledoyen]

Marin Biologic Laboratories, a leading contract research organization in the field of biological and biopharmaceutical development, proudly announces the successful completion of a U.S. Food and Drug Administration (FDA) inspection for commercial drugs at its state-of-the-art facility in Novato, California. The rigorous inspection confirms Marin Biologic Laboratories’ compliance with Current Good Manufacturing Practices (cGMP). The rigor of cGMP for commercial drugs is significantly greater than that for early clinical phase pharmaceuticals.

[lovelypol]

"Unlocking Insights: Exploring the Power of Cell-based Assays 🧫"

Cell-based assays are invaluable tools in biomedical research, providing researchers with a window into cellular function and behavior.

These assays utilize living cells to study various biological processes, including cell viability, proliferation, and response to stimuli or treatments. From drug discovery to disease modeling and beyond, cell-based assays offer a versatile platform for investigating complex biological systems. With advancements in imaging technology, automation, and high-throughput screening, researchers can conduct large-scale experiments efficiently, accelerating the pace of discovery. Let's harness the power of cell-based assays to unlock new insights into health and disease! #CellBasedAssays #BiomedicalResearch #CellularFunction #DrugDiscovery #DiseaseModeling #HighThroughputScreening #Biotechnology #CellViability #ResearchTechniques #Innovation #BiologicalSystems #HealthScience #LaboratoryMethods #ScientificDiscovery #CellularResponses

[nickyhants]

Have you seen our new product, the LAG-3/MHCII Blockade Bioassay? Measure potency and stability of biologics designed to block the LAG-3 interaction with MHCII. • Prequalified according to ICH guidelines • Predictive of true biology • Amenable to high-throughput formats More on our website (link in bio) under products and reporter bioassays (product codes JA1111 and JA1115). . . . #LabChat #PhDChat #bioassay #PromegaUK #biologics #LabWork #biology #science #biologists #scientists #bioassays #hightthroughput #MHCII #LaboratoryWork #blockadebioassay #bioluminescent #cellbasedassay #assays #LAG3 #histocompatibility #TCRsignaling #TCells #cancerresearch #oncologyresearch #assay #ligand #immunecheckpoint #cellhealth #reagents #cellcount https://ift.tt/2ZA1UKl

[companiesandmarkets]

The quest for increased biological relevance has propelled different technologies. Cell-based assays are becoming more important as companies realize their usage.

[elainaledoyen]

While cell-based assays are considered a different section of life sciences, they have found application and optimum use in many disciplines. Explore more about cell-based assays, their relevance, and their benefits.

[elainaledoyen]

Cell-based assays assume a crucial role in a string of research across biomanufacturing and drug discovery. As the pharmaceutical industry continues to tackle the challenge of a high attrition rate in drug development (due to toxicity), the role of a cell-based assay can’t be stressed enough. A typical cell-based assay takes into consideration live cells, in vitro to be used as a model system for the assessment of physiology and biochemistry of healthy as well as diseased cells. In essence, they offer a quantitative analysis regarding the presence and functioning of a specific cell tissue that is of interest. Cell-based assays have gained widespread adaptability as a reproducible and on-budget tool to aid multiple medical studies. They are widely used in the shape of standardized as well as custom-made solutions for multiple applications, like 3D cell culture, pre-clinical trials, pluripotent stem cell study, promoting compound efficiency, and toxicity screening. This post explores more on cell-based assays, the commonly used types, and their ever-increasing significance in today’s medical world.

[elainaledoyen]

If you’re venturing into the world of life science research, drug discovery, and other related fields, you will come across the concept of cell-based assay. For the uninitiated, these are sophisticated techniques used to study biological processes. Researchers also rely on cell-based assays to study disease mechanisms and drug effects on living cells. While it is not new, its widespread usage in recent times has made it popular for the right reasons. It has entirely revolutionized the way professionals approach diagnostic testing, biomedical research, drug development, etc. More pharmaceutical companies and researchers are turning to cell-based assays to accelerate their work due to their efficiency. If you’re interested to learn more about cell-based assays, especially what they’re used for, this article is for you. If you’re ready to explore the numerous applications of cell-based or cellular assays, read along.

[elainaledoyen]

Drug development would be impossible without assays. But, what are assays exactly? Assays, in short, are tests. Bioassays are the specific kinds of assays that are required in drug development. So, what kind of tests are bioassays? In short, these are tests that are used to determine the potency and presence of any particular active ingredient in a drug. Are you wondering about the core principle behind these bioassays? Bioassays work because they recognize and measure the effect that an active ingredient has on the tissues or cells that it acts on.

[elainaledoyen]

Drugmakers must always make sure that the drugs that they are pushing out into the market are not toxic. This is why cell-based assays, especially the ones meant to measure cytotoxicity, are so important for drug manufacturers. Moreover, now there is a method to the process of discovering which active ingredients yield favorable results. Scientists don’t have to stumble across these accidentally anymore. Previously the process of isolating this active ingredient was extremely difficult. And then hundreds of tests had to be performed on live subjects to accidentally stumble across the optimal proportion in which the active ingredient was needed in the drug. Now that optimal proportion can easily be measured through cell-based assays.

[elainaledoyen]

Recombinant monoclonal antibodies are one of the most promising drugs (immunotherapy) for human diseases which include infectious diseases, cancer, and various other diseases. Mammalian cells (CHO, HEK293, PER.6) are primarily being used to produce therapeutic antibodies, except single chain antibodies without PTMs (glycosylation) which are expressed in non-mammalian cells or cell-free expression systems.  Generation of antibody producing stable cell lines is the single most factor for large-scale manufacturing of therapeutic antibodies. There are several critical factors that are associated with generating stable cell lines for expressing therapeutically active recombinant antibodies. These factors include engineering of antibodies, vectors, and mammalian cells.

[elainaledoyen]

Assays are essentially tests. Bioassays are tests that are used to measure the potency of an active ingredient in the drug by measuring its effects on cells, tissues, or living animals. Cell-Based Assays, as the name suggests, are tests that are performed within a cell. Cell-Based Assays are conducted for several reasons. Sometimes the cytotoxicity of a substance (that is, whether the substance is toxic to the cells) is measured. Other times, as mentioned before, they are used to test at what concentration a substance produces the optimal effects in the test subject. A variety of parameters can be measured through a cell-based assay: radioactivity, luminescence, absorbance, fluorescence, etc. The key feature of a cell-based assay is that it must produce a detectable signal so that the parameter being measured can be determined. Cell-based assays employ a series of reagents for this purpose. What matters the most is the robustness of the signal. It is also important that the signal does not go off with the control experiment when the standard procedure is being tested. However, the same detectable signal must be produced when the same concentration of the substance is tested again. What this means is that the reproducibility of the assays is a necessary factor.

[elainaledoyen]

Detection of mutations arising in the genome of the SARS-CoV-2 virus responsible for COVID-19 have led to concerns that new forms of the virus might enable it to evade the protection conferred by recently approved mRNA vaccines. Two strains in particular, B.1.1.7 from England and B.1.351 from South Africa, are driving the unease. B.1.1.7, first reported in September 2020, has been shown to be more transmissible than the previous strain and is spreading globally. The South African mutant was reported in mid-December 2020 by health authorities there as becoming the chief variant, an indication of heightened transmissibility. This strain garnered additional attention because the portion of Novavax’s Phase 3 vaccine trial conducted in South Africa showed noticeably lower efficacy there than in the UK portion.

[elainaledoyen]

Several cell-based assays are now being used to determine the correlates of protection in groups and individuals with symptomatic COVID-19 immunity and vaccine reactions. The cell-based assays have demonstrated high levels of concordance with living as well as pseudotyped virions of the novel coronavirus. For a return to normalcy and the resumption of economic activity across the globe, national borders will need to be reopened and businesses will have to start functioning as before. However, for this to happen, safe and effective vaccines, as well as other treatment modalities, will have to be developed and implemented as soon as possible. Hence, scientists need to be able to appraise the factors that determine protective immunity against the SARS-CoV-2 virus, also known as the severe acute respiratory syndrome 2. This is because the SARS-CoV-2 is known to be a major causative agent of COVID-19.

[elainaledoyen]

The successful development and use of cell-based assays can have a huge impact on the cost-effectiveness of pharmaceutical research. Evaluating the biological effects of chemical compounds requires multiple different types of approaches, including molecular, cellular, and biochemical. The first step towards such evaluation includes the successful execution of cell-based and in vitro assays. This article talks about the importance of high-quality cell-based assay development while highlighting six ways to make the process less time-consuming and more cost-effective.

[elainaledoyen]

Some kits are suitable, but will they fit your purpose? Should you use a kit for an ELISA, cell based assay, and enzyme assay or even a PCR project? Some kits are appropriate for certain applications. The advantage of a kit is that it typically costs less, since development is already completed by the vendor. However, some kits run over $1000/kit, and could be manufactured by MarinBio much more economically. Consideration as to which vendor manufactures the kit is important, as availability and reproducibility is essential and not all vendors offer high quality products with expected reproducibility from lot to lot. Since it is critical to have reproducible data for lot release assays and sample stability projects over years, vendor and kit selection as well as availability of the kit is extremely important. MarinBio has experience with many off-the-shelf kits and can determine whether they are suitable or whether an adaption or a better method should be developed.