#Data Extractor from H&M

[3idatascraping]

How to Extract Products Data from H&M with Google Chrome

Data You Can Scrape From H&M

Product’s Name

Pricing

Total Reviews

Product’s Description

Product’s Details

The screenshot provided below indicates various data fields, which we scrape at 3i Data Scraping:

Requests

Google’s Chrome Browser: You would require to download the Chrome browser and the extension requires the Chrome 49+ version.

Web Scraping for Chrome Extension: Web Scraper extension could be downloaded from Chrome’s Web Store. Once downloaded the extension, you would get a spider icon included in the browser’s toolbar.

Finding The URLs

H&M helps you to search products that you could screen depending on the parameters including product types, sizes, colors, etc. The web scraper assists you to scrape data from H&M as per the requirements. You could choose the filters for data you require and copy corresponding URLs. In Web Scraper toolbars, click on the Sitemap option, choose the option named “Edit metadata’ to paste the new URLs (as per the filter) as Start URL.

For comprehensive steps about how to extract H&M data, you may watch the video given here or continue to read:

Importing H&M Scraper

After you install the extension, you can right-click anyplace on the page and go to the ‘Inspect’ option as well as Developer Tool console would pop up. Just click on the ‘Web Scraper’ tab and go to the ‘Create new sitemap’ option as well as click on the ‘Import sitemap’ button. Now paste JSON underneath into Sitemap’s JSON box.

Running The Scraper

To start extracting, just go to the Sitemap option and click the ‘Scrape’ alternative from the drop-down menu. Another window of Chrome will come, permitting the extension for scrolling and collecting data. Whenever the extraction is completed, the browser will be closed automatically and sends the notice.

Downloading The Data

For downloading the extracted data in a CSV file format, which you could open with Google Sheets or MS Excel, go to the Sitemap’s drop-down menu > Export as CSV > Download Now.

Disclaimer: All the codes given in our tutorials are for illustration as well as learning objectives only. So, we are not liable for how they are used as well as assume no liabilities for any harmful usage of source codes. The presence of these codes on our website does not indicate that we inspire scraping or scraping the websites given in the codes as well as supplement the tutorial.

[ballpiner]

Knowledge graph builder

Miller, A., Fisch, A., Dodge, J., Karimi, A.H., Bordes, A., Weston, J.: Key-value memory networks for directly reading documents. In: Proceedings of the 7th International Conference on Semantic Systems, pp. Mendes, P.N., Jakob, M., García-Silva, A., Bizer, C.: Dbpedia spotlight: shedding light on the web of documents. Ling, X., Singh, S., Weld, D.S.: Design challenges for entity linking. Li, J., Sun, A., Han, J., Li, C.: A survey on deep learning for named entity recognition. In: Proceedings of the 14th ACM International Conference on Web Search and Data Mining, pp. He, G., Lan, Y., Jiang, J., Zhao, W.X., Wen, J.R.: Improving multi-hop knowledge base question answering by learning intermediate supervision signals. Haveliwala, T.H.: Topic-sensitive pagerank. arXiv preprint arXiv:2007.13069 (2020)įu, K., et al.: Ts-extractor: large graph exploration via subgraph extraction based on topological and semantic information. 65(5), 891–904 (2014)įu, B., Qiu, Y., Tang, C., Li, Y., Yu, H., Sun, J.: A survey on complex question answering over knowledge base: recent advances and challenges. arXiv preprint arXiv:2108.08597 (2021)įensel, A., Toma, I., García, J.M., Stavrakantonakis, I., Fensel, D.: Enabling customers engagement and collaboration for small and medium-sized enterprises in ubiquitous multi-channel ecosystems. 1247–1250 (2008)Ĭhristmann, P., Roy, R.S., Weikum, G.: Beyond ned: fast and effective search space reduction for complex question answering over knowledge bases. In: Proceedings of the 2008 ACM SIGMOD International Conference on Management of Data, pp. 1533–1544 (2013)īollacker, K., Evans, C., Paritosh, P., Sturge, T., Taylor, J.: Freebase: a collaboratively created graph database for structuring human knowledge. In: Proceedings of the 2013 Conference on Empirical Methods in Natural Language Processing, pp. 1431–1440 (2015)īerant, J., Chou, A., Frostig, R., Liang, P.: Semantic parsing on freebase from question-answer pairs. In: Proceedings of the 24th ACM International on Conference on Information and Knowledge Management, pp. 967–976 (2014)īast, H., Haussmann, E.: More accurate question answering on freebase. In: Proceedings of the 52nd Annual Meeting of the Association for Computational Linguistics (Volume 1: Long Papers), pp. 146, 113205 (2020)īao, J., Duan, N., Zhou, M., Zhao, T.: Knowledge-based question answering as machine translation. 4(1), 35–64 (2007)īakhshi, M., Nematbakhsh, M., Mohsenzadeh, M., Rahmani, A.M.: Data-driven construction of sparql queries by approximate question graph alignment in question answering over knowledge graphs. IEEE Access 8, 149220–149230 (2020)Īndersen, R., Chung, F., Lang, K.: Using pagerank to locally partition a graph. 1191–1200 (2017)Īit-Mlouk, A., Jiang, L.: Kbot: a knowledge graph based chatbot for natural language understanding over linked data. In: Proceedings of the 26th International Conference on World Wide Web, pp. Abujabal, A., Yahya, M., Riedewald, M., Weikum, G.: Automated template generation for question answering over knowledge graphs.

[sourcingblog567]

Winchester Model 74 Automatic Manual

Winchester Model 74 For Sale

Winchester Model 74 Automatic Manual For Sale

Winchester Model 74 Assembly Instructions

Winchester Model 74 Owners Manual

Winchester 74 Schematic

Winchester Model 74 Automatic Manual Automatic

Winchester Model 74 Schematics

Hoosier GunWorksOnline Catalog( Catalog Index )Gun Parts WInchester ( M-12| M-37 | M-52 |M-63|M-94 | M-97 | M-1890| M-1400 | M-1200 | M-70 | M-52 | M-67/68| )

Winchester Closed the US plant on March 31, 2006 No more US made Winchesters. Parts will be scarce for most models.

Update:2010 Winchester Model 70's are now being made in the US No other Winchester are being made currently made in the US.

To place an order. SeeOrdering link at bottom of page. Sorry no On Lineordering

Values for.Winchester Model 74.22 Caliber Rifle.22 cal. 23' barrel S/N 107783. 1940 year of production. Condition: Very good. To appraise similar items instantly without sending photos or descriptions. Dates of Production by Model and Serial Number for Model 74.

Winchester Model 74 For Sale

Model -12DescriptionConditionQuantity Available Price M-12 TRIGGER GUARD STRIPPED 12 GA VG2 25.00M-12 TRIGGER GUARD COMPLETE12 GA VG0 50.00M-12 RH EXTRACTOR 12 GA 110.00M-12 BOLT STRIPPED 12 GA ImageVG 125.00M-12 BOLT STRIPPED 12 GA (JEWELED)VG 125.00M-12 BOLT COMPLETE 16 GA EXC 1 60.00 M-12 ACTION SLIDE LOCK 12 GA EXC 020.00M-12 CARRIER 12 GA EXC 230.00M-12 MAG LOCKING PINNEW0 10.00M-12 MAG BAND SCREWNEW0 5.00M-12 MAG BAND 12 GAVG 0 10.00M-12 MAG BAND 16 GAVG 1 10.00M-12 MAG BAND BUSHINGVG0 5.00M-12 FIRING PIN 20 GA 012.00M-12 FIRING PIN 20 GA 0 12.00 M-12 SAFETY 12 GA 110.00M-12 CARTRIDGE CUT OFF 015.00M-12 EJECTOR 16 GA 010.00M-12 EJECTOR 12 GA 010.00M-12 MAGAZINE PLUG EARY DOMED ImageVG 1 15.00M-12 MAGAZINE BAND SCREWEXC 0 2.00M-12 ACTION SLIDE 20 / 16 GA VG 0 35.00M-12 ACTION SLIDE 12GA VG 2 35.00 M-12 TAKE DOWN EXTENTIONON PARTIAL BARREL EXC 0 45.00 M-12 TRIGGER GUARD 20 / 16 GA COMPLETE ImageVG 0 40.00M-12 BOLT 16 GA COMPLETEVG 0 55.00M-12 CARRIER 20 / 16 GANEW 0 35.00Model -37DescriptionConditionQuantity AvailablePriceWIN M-37 HAMMER NEWER STYLE 015.00WIN M-37 TRIGGER NEW STYLE 010.00WIN M-37 FIRING PIN 12 GA 015.00WIN M-37 EXTRACTOR 12 GA 010.00WIN M-37 SEAR BLOCK 010.00WIN M-37 TOP LEVER NEW TYPE 010.00WIN M-37 FOREARM SHOE STUD 08.00WIN M-37 FOREARM SHOE RETAINER 010.00 WIN M-37 / 37A FOREARM SPACER, PLASTICNEW 0 10.00 WIN M-37A FOREARM SPACER,STEELNEW 0 15.00 WIN M-37A FOREARM SPACER,STEELEXC 0 10.00 WIN M-37A HAMMER 1 15.00 WIN M-37A TRIGGER 1 10.00 WIN M-37A FIRING PIN 2 10.00 WIN M-37A FIRING PIN SCREW 1 2.00 WIN M-37A TOP LEVER 1 12.00 WIN M-37A TRIGGER GUARD VG 1 12.00 WIN M-37A LOCKING BOLT 1 8.00 Model -52DescriptionConditionQuantity AvailablePriceTRIGGERGUARD M-52CEXC 0 20.00MAGAZINE HOLDER ASSEMBLY M-52CEXC 0 25.00Model 63 DescriptionConditionQuantity AvailablePriceTANG WITH INNER MAGAZINE TUBEEXC 0 75.00FORE TIPEXC 0 10.00Model -1890DescriptionConditionQuantity AvailablePriceM-90 HAMMERVG0 25.00M-90 HAMMERGD0 15.00M-90 HAMMER MAINSPRINGVD0 10.00M-90 FINGER LEVER 120.00M-90 TAKEDOWN SCREW 08.00M-90 TAKEDOWN SCREW BUSHING 05.00M-90 FIRING PINEXC0 15.00M-90 SLIDEVG 115.00M-90 CARRIER, 22 SHORTVG 035.00M-90 MAGAZINE RING (FRONT/REAR) SET OF 2GD 0 10.00M-90 BOLT (STRIPPED) EARLYVERSION VG 2 40.00 Model -94DescriptionConditionQuantity AvailablePriceWIN M-94 STEEL CRESENT PLATEVG0 25.00WIN M-94 MAG TUBE ASSEMBLY 30-30 post 64EXC 1 45.00WIN M-94 MAGCAP 11394 NEW1 10.00WIN M-94 MAGAZINEFOLLOWER 5794 VG 3 10.00 WIN-94 FRONT BARRELBAND 189694 NEW 1 15.00 WIN-94 REAR BARRELBAND 1519 NEW 0 15.00 WIN-94 CARRIERSPRING 1494 NEW 1 WIN-94 CARRIER SPRINGCOVER (LOADING GATE) 6994 NEW 1 WIN-94 CARTRIDE GUIDELEFT 1794 NEW 1 WIN-94 CARTRIDE GUIDERIGHT 1694NEW 1 WIN-94 CARTRIDGE GUIDESCREW 1894 NEW 1 WIN- 94 FINGER LEVER LINKSCREW 3493 WIN M-94 AE 356 BOLT ASSEMBLYVG 1 50.00WIN M-94 AE 356 CARRIERVG 0 20.00WIN M-94 AE FINGER LEVERGD 0 35..00WIN M-94 AE HAMMERVG 0 25.00 WIN M-1873 MAG PLUGVG 1 25.00 M-94 -22 FINGER LEVEREXC 0 25.00M-94-22 STOCK BOLTEXC 0 5.00M-94 -22 BOLTEXC 0 10.00M-94 -22 FIRING PINVG 0 15.00M-94 -22 TRIGGEREXC 0 8.00M-94-22 HAMMEREXC 0 22.00 M-94-22 EXTRACTORLEFT 2149 NEW 5 M-94-22CARRIER 1049 NEW 1 M-94-22 CARRIERSPRING 1649 NEW 1 M-94-22 CARRIERPAWL 1149 NEW 1 M-94-22 CARTRIDGE CUTOFF 1749 NEW 1 M-1892 CARRIER CALIBER? ImageEXC 1 45.00 M-1892 HAMMER SLIGHTPITTING ImageVG 1 40.00 Model -97 If marked NEWit is Old in New condtion Image DescriptionConditionQuantity AvailablePrice ea IF MARKED NEW IT IS OLD STOCK, FROM WAY BACK WHEN M-97 FORE ARM SCREWS, FOR 3 SCREW TYPE OLD STYLE NEW0 4.00M-97 ACTION SLIDE, 3 SCREW, 16 GA ImageGD 1 35.00M-97 ACTION SLIDE FIXED TUBE MODEL MARKED 1897 THREE LINES. ImageVG 0 35.00M-97 ACTION SLIDE SLEEVE SCREW CAPNEW1 12.00M-97 ADJUSTING SLEEVE LOCK ImageNEW5 8.00M-97 ACTION SLIDE LOCK ImageNEW5 20.00 M-97 ACTION SLIDE LOCK RELEASE SPRING ImageNEW 3 5.00M-97 ACTION SLIDE HOOKVG2 10.00M-97 ACTION SLIDE RELEASE PLUNGER ImageNEW4 10.00M-97 ACTION STOPSCREW ImageNEW 4 5.00 M-97 ACTION SLIDEESCUTHCHEON VG 3 5.00 M-97 MAG TUBE FIXED TYPEVG0 40.00M-97 MAG CAP FIXED, NON TAKEDOWN ImageNEW0 25.00M-97 MAG CAP TD OLD STYLE VG 0 15.00 M-97 MAG CAP TDNEW 0 15.00M-97 MAG CAP SCREWNEW0 3.00M-97 MAG CAP BUSHING ImageNEW0 5.00M-97 MAGAZINE PLUG STOP NEW 2 6.00 M-97 BARREL CHAMBER RING ImageNEW2 10.00M-97 MAGAZINE BAND ImageVG2 20.00M-97 MAGAZINE BANDBUSHING ImageNEW 2 6.00 M-97 MAGAZINE BAND BUSHING SCREW ImageNEW 1 4.00 M-97 CARRIER PIN ImageNEW 6 5.00 M-97 BOLT STRIPPED ImageVG 4 25.00 M-97 BOLT STRIPPEDEXC 0 35.00M-97 BOLT STRIPPED ImageNEW0 45.00M-97 LH EXTRACTOR ImageNEW0 24.00M-97 RH EXTRACTORVG1 15.00M-97 RH EXTRACTORSPRING ImageNEW 4 4.00 M-97 CARRIER STRIPPED, GOOD CONDITION NOT GREAT ImageVG0 25.00 M-97 MAG LOCK W/ SPRINGNEW0 13.00M-97 MAG LOCK Image NEW0 10.00M-97 MAG LOCK SPRING ImageNEW2 4.00M-97 TRIGGERGUARD W/ SCREW ImageVG 4 20.00M-97 TRIGGER STOP SCREW Image NEW4 4.00M-97 TRIGGERVG0 10.00M-97 TRIGGER PINNEW3 3.00M-97 SEAR VG 0 10.00 M-97 SEAR SPRING ImageNEW5 8.00M-97 HAMMER PIN NEW0 5.00 M-97 HAMMERGD 0 15.00M-97 MAINSPRING ImageNEW 1 10.00 M-97 MAINSPRING SCREW NEW 2 3.00 M-97 EJECTOR SPRING ImageNEW3 5.00M-97 EJECTOR SCREW ImageNEW 4 3.00 M-97 RH CARTRIDGE STOP (PUSH BUTTON) ImageNEW 0 12.00M-97 LH CARTRIDGE STOP (PUSH BUTTON) NEW 0 12.00M-97 RH CARTRIDGE STOP VG 3 10.00 M-97 LH CARTRIDGE STOP Image VG0 10.00 M-97 MAGAZINE END CAPVG 2 10.00 M-97 MAGAZINE PLUG STOP VG 2 8.00 M-97 MAGAZINE FOLLOWER VG 1 7.00 M-97 MAGAZINEFOLLOWER SOLID FRAME ImageNEW 1 10.00 M-97 FIRING PIN Image VG6 12.00 M-97 FIRING PIN OLDER FLAT SHAFT (93/97) ImageVG 2 12.00 M-97 FIRING PIN LOCK Image NEW4 8.00 M-97 MISC PARTS ImageImageImage M-1300/ 1200 / 120 12 GA DescriptionConditionQuantity AvailablePriceM-1200 BREECH BOLT COMPLETE EXC 0 45.00 M-1200 BREECH BOLT STRIPPED VG 025.00M-1200 SLIDE BRIDGE ARM EXC 0 20.00 M-1200 SLIDE ARM EXTENSION 4 3/4' EXC 0 25.00 M-1200 EJECTOR 12 GA EXC1 15.00M-1200 TRIGGERGUARD COMPLETE WITH LIFTER EXC 1 65.00 M-1200 TRIGGER GUARD STRIPPED ALLOY VG 025.00M-1200 TRIGGERGUARD PIN EXC 2 4.00 M-1200 FIRING PIN 12 GANEW 010.00M-1200 ACTION EXTENSION SHORT EXC 0 35.00 M-1200 MAGAZINE CAP EXC 1 15.00 M-1500/ 1400 / 140 DescriptionConditionQuantity AvailablePriceM-1400 TRIGGER PLATE ASSEMBLYCOMPETE VG 1 6.00 M-1400 CARRIER ASSEMBLY VG 1 25.00 M-1400 EJECTOR 12 GA EXC 1 15.00 M-1400 DISCONECTOR SPRING NEW 1 5.00 M-1400 BREECH BOLT STRIPPEDVG 1 25.00 M-1400 COCKING HANDLE BRIDGE EXC 0 25.00 M-1400 FEED THROAT UPPER 20 GA NEW 0 10.00 M-1400 FEED THROAT LOWER 20 GANEW 0 10.00 M-320DescriptionConditionQuantity AvailablePriceWIN M-320 TRIGGER GUARD VG2 18.00WIN M-320 TRIGGERGUARD RUSTY SPOTS GD 4 12.00 Model -70 / 54 DescriptionDescriptionQuantity AvailablePriceWIN 70 TRIGGER ASSEMBLY EXC 0 25.00 WIN 70 TRIGGER BOWEXC 120.00 WIN 70 TRIGGER BOW PRE-64 EXC 0 35.00 WIN 70 FLOORPLATE PRE-64 MAGNUM VG 0 40.00 WIN 70 FLOORPLATE PRE-64 VG 0 35.00 WIN 70 BOLT BODY PUSH FEED VG 0 75.00 FN TST WSM TRIGGERGUARD / WI NM-70 WSM 1PIECE EXC 1 75.00 WINCHESTER M-54 SAFTEY Image VG1 45.00 M-190DescriptionDescriptionQuantity AvailablePriceFRONT SIGHT HOUSING METAL NEW 1 25.00 Model -67 / 68DescriptionConditionQuantity AvailablePriceBOLT 22 LRVG 0 100.00 TRIGGERVG 0 7.00 TRIGGERGUARDVG 0 12.00 SEAR BAR VG 0 10.00 MISCWIN M-59 12 GA FIRING PIN ImageEXC 1 15.00 SEE BARRELSSEE Stocks

( Home | Catalog| About Us | News/Specials| Links | ContactUs )Images and photos©Copyright 1999-2020 Hoosier Gun Works || Site created by HGW

Whenever we can find an interesting original firearm manual, we’ll scan it and post it here. If you have a manual you think ought to be included here, please contact us!

(1943) Oerlikon 20mm AA gun US Ordnance Pamphlet (English)

(1918) Vickers Mk VI 8″ Howitzer manual (English)

(1942) Boys AT Rifle Small Arms Training Manual (English)

(1942) Bren Small Arms Training (South Africa) (English)

Bren Light Machine Gun – Description Use and Mechanism (English)

(1908) Sjogren system article, Engineering magazine (English)

Hanyang report on the General Liu Rifle (Chinese)

Winchester-Lee straight pull rifle handbook (commercial) (English)

(1896) M1895 Navy Lee manual (English)

(1907) Ross MkI & MkII manual (English)

(1950) Provisional Notes for users of Rifle, Automatic, .280-in, E.M.1 (English)

Winchester Model 74 Automatic Manual For Sale

FN Model 49 Operator’s Manual (English)

(1950) Prototype FN FAL manual (English)

FN-FAL Operator’s Manual (English)

FAMAS F1 manual (English)

FAMAS F1 manual (French)

(1942) M1A1 Carbine Base Shop Data (English)

(1958) Winchester 224 E2 rifle manual (English)

(1965) H&R T223 rifle manual (English)

Stoner 63 System sales brochure (English)

Stoner 63A rifle manual (English)

Stoner 63A LMG manual (English)

Stoner 63 MMG manual (English)

Annex to Service and Troop Tests, Stoner 63 System (English)

Cadillac-Gage Engineering Change Report for Stoner 63A (English)

(1984) Ultimax 100 MkIII manual (English)

Winchester Model 74 Assembly Instructions

Pedersen Handbook,Vickers-Armstrong Company (English)

(1902) Mars Pistol manual (English)

Winchester Model 74 Owners Manual

(1937) Webley revolver training manual (English)

(1914) Colt 1911 Manual (English)

(1960) Tiro Para Pistola (Shooting the Pistol) (Spanish)

(1927) Bang Model B1 Rifle (English)

(1930) Czech ZH29 Manual (English)

(1943) G41(M) manual (German)

KE7 Factory overview (Spanish)

KE7 Factory overview (French)

KE7 Manual (German)

ZB 26 manual (English)

(1940) MG26(t) parts list (German)

Factory ZB30 Manual (English)

ZB30 Fusil Ametrallador (Spanish)

(1916) Villar Perosa M1915 manual (Italian)

(1918) Provisional Instruction on the Automatic Rifle, Model 1915 (Chauchat) (English)

(1878) Gattling Gun, Caliber 45 Manual (English)

(1917) Colt 1895 manual (English)

(1918) Notes on Mechanism of the Chauchat Machine Rifle, Model 1915 (English)

(1918) Manual of the Automatic Rifle (Chauchat), Drill – Combat – Mechanism (English)

Model 1918 Lewis gun manual (English)

(1917) US Model 1898/1899 Krag Manual (English)

(1943) Browning 1917 shop manual (English)

(1943) M1918A2 BAR Base Shop Data (English)

(1940) Handbook of the Johnson Automatic Rifle (English)

US .30-06 Type 99 Arisaka conversion manual (English)

(1965) Instrukcja 7,62 mm KARABINEK wz. 1944. wz. 1938 (Polish)

(1954) Instruckja PISTOLET MASZYNOWY wz. 1941, wz. 1943 i wz. 1943/52 (Polish)

(1961) Instruckja PJSTOLET wz 1933 (Polish)

(1928) Fedorov Avtomat manual (Russian)

(1941) Russian MG34 manual (Russian)

(1944) Russian MG42 manual (Russian)

Winchester 74 Schematic

(1972) Russian SKS manual (Russian)

(1956) PPSh-41/PPS-43 Armorer’s manual (Russian)

(1947) 1910 Maxim manual (Russian)

(unk) RPD manual (Russian)

Winchester Model 74 Automatic Manual Automatic

(1954) RPD Armorer’s manual (Russian)

(1949) RP-46 manual (Russian)

(1962) DTM manual (Russian)

DsHK 38 Manual (Russian)

NSV 12.7mm Technical Manual (Russian)

(1975) MSP Groza silent pistol manual (Russian)

(1972) RPG-7 Manual (Russian)

Madsen Saetter GPMG manual (English)

Madsen M/50 SMG Handbook (English)

Carl-Gustaf Submachine Gun manual (English)

Winchester Model 74 Schematics

Madsen Machine Rifle Main Characteristics & Tactical Use (English)

Madsen Nomenclature (multiple languages)

Madsen 1904/1912 comparison (English)

Description of the Madsen Model 1940 (English)

(1938) Madsen Manual, mostly 20mm (German)

Madsen Argentine Model 26 Nomenclature (Spanish)

Ameli LMG Descriptive Handbook (English)

Vickers Class F Commercial Manual (English)

(1902) Mannlicher m/1901 Selbstlade-Karabiner (German)

[adalidda]

Illustration Photo: Cassava Mosaic Disease and Cassava Brown Streak Disease (credits: International Institute of Tropical Agriculture / Flickr Creative Commons Attribution-NonCommercial-ShareAlike 2.0 Generic (CC BY-NC-SA 2.0))

Tree Lab: Portable Genomics for Early Detection of Plant Viruses and Pests in Sub-Saharan Africa

Authors: Laura M. Boykin,Peter Sseruwagi, Titus Alicai, Elijah Ateka, Ibrahim Umar Mohammed, Jo-Ann L. Stanton, Charles Kayuki, Deogratius Mark, Tarcisius Fute, Joel Erasto, Hilda Bachwenkizi, Brenda Muga, Naomi Mumo, Jenniffer Mwangi, Phillip Abidrabo, Geoffrey Okao-Okuja, Geresemu Omuut,Jacinta Akol, Hellen B. Apio, Francis Osingada, Monica A. Kehoe, David Eccles, Anders Savill, Stephen Lamb, Tonny Kinene, Christopher B. Rawle, Abishek Muralidhar, Kirsty Mayall, Fred Tairo and Joseph Ndunguru

Journal: Genes 2019, 10(9), 632;

Publisher: MDPI

In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with the MinION and MinIT mobile sequencing devices developed by Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The PDQeX extracts DNA using a cocktail of thermophilic proteinases and cell wall-degrading enzymes, thermo-responsive extractor cartridges and a temperature control unit. This closed system delivers purified DNA with no cross-contamination. The MinIT is a newly released data processing unit that converts MinION raw signal output into nucleotide base called data locally in real-time, removing the need for high-specification computers and large file transfers from the field. All three devices are battery powered with an exceptionally small footprint that facilitates transport and setup. To evaluate and validate capability of the system for unbiased pathogen identification by real-time sequencing in a farmer’s field setting, we analysed samples collected from cassava plants grown by subsistence farmers in three sub-Sahara African countries (Tanzania, Uganda and Kenya). A range of viral pathogens, all with similar symptoms, greatly reduce yield or destroy cassava crops. Eight hundred (800) million people worldwide depend on cassava for food and yearly income, and viral diseases are a significant constraint to its production. Early pathogen detection at a molecular level has great potential to rescue crops within a single growing season by providing results that inform decisions on disease management, use of appropriate virus-resistant or replacement planting. This case study presented conditions of working in-field with limited or no access to mains power, laboratory infrastructure, Internet connectivity and highly variable ambient temperature. An additional challenge is that, generally, plant material contains inhibitors of downstream molecular processes making effective DNA purification critical. We successfully undertook real-time on-farm genome sequencing of samples collected from cassava plants on three farms, one in each country. Cassava mosaic begomoviruses were detected by sequencing leaf, stem, tuber and insect samples. The entire process, from arrival on farm to diagnosis, including sample collection, processing and provisional sequencing results was complete in under 3 h. The need for accurate, rapid and on-site diagnosis grows as globalized human activity accelerates. This technical breakthrough has applications that are relevant to human and animal health, environmental management and conservation.

Check more https://adalidda.com/posts/S69CRvjFnF5iaNHts/tree-lab-portable-genomics-for-early-detection-of-plant