#m1A modification

[rnomics]

RMDGCN: Prediction of #RNA methylation and disease associations based on graph convolutional network with attention mechanism

#RNA modification is a post transcriptional modification that occurs in all organisms and plays a crucial role in the stages of #RNA life, closely related to many life processes. As one of the newly discovered modifications, N1-methyladenosine (m1A) plays an important role in gene expression regulation, closely related to the occurrence and development of diseases. However, due to the low abundance of m1A, verifying the associations between m1As and diseases through wet experiments requires a... https://pubmed.ncbi.nlm.nih.gov/38055721/?utm_source=dlvr.it&utm_medium=tumblr&utm_campaign=None&utm_content=1RYYbE7j9SUSBe_aHniaI_J1MQIFIBbfLuFxoWdLNMNDzVVIWF&fc=None&ff=20231216100537&v=2.18.0

[athomewiththecicadas]

Oldies but goodies. Hoo?

My Springfields?

M1A Standard rifle.

I had a new barrel poured in H22 tool steel. Along with a standard bolt. And that’s from all the metalworking when I was younger. No tricks, just tool steel.

As for machining, tool steel doesn’t bend as much, which allows me to loosen the rifling. It’s not that much faster than a standard barrel, which puts it back to standard specs. And the bolt clearances were also loosened as well. But both together the barrel and the bolt we’re talking 1000th’s of an inch. 

If you ever get the opportunity to watch an M1A fire in slow motion, you can see it wobbles. But it was cheap. Maybe a $200-$300 hundred dollars.

Bushnell/Nikon

But I did run across a muzzle break for an M1 Garand and got it to fit an M1A.

Monster Energy Drink break**

Anyway, I bought a Springfield XDM .40 when they first came out, and when you think of buying a nice handgun, I would think Sig Sauer. You know you expect it to hit the target in the indoor range, and then the machining of the handgun is supposed to prop up your firing position. But I bought it blind. Never shot one.

And it took some getting used to because it was sensitive to my index finger. And it took me about 8 magazines to get the hang of it after I went and asked for help, because I started to think I might need to get it set up or adjusted.

Nope, he points out that it’s really that sensitive. So, I bought a gun that embarrassed me instead. And it’s got a plastic frame. Imagine that.

And so, I held onto it for a few years, and cleaning the XDM turns into a religious experience, which for an officer is also odd. And you would think that the brand-new feeling would dull, but here’s where it doesn’t embarrass you:

It never losses it’s value for display. And I have to admit I actually bought it.

But it’s definitely my Type ‘56 SKS. And for 20 years it’s been my Chinese 1956 SKS. But if you were allowed to do anything you wanted to your rifle, I’m still debted for the firing range. And I am an expert marksman.

So, hang on a second. That is a really weird question to ask someone who shoots for competition. There are no modifications to speak of for “martial” competition. But, ah yes, all of my mastery of range rifles for competition.

I’ve got a thumbhole stock. And I actually got it turned in raw black walnut. And if that isn’t enough, I got it turned noting my bone structure. And if you shoot rifles, you would notice after a few magazines that for some reason that you can’t see: “this rifle feels good.”

But it’s not just a raw black walnut thumbhole stock, the grain is “diamond cut,” just like you’re looking at those arrowheads for archery. (on-going)

It’s my thumbhole stock.

And so, when you are thinking about changing calibers from 7.62x39 to 7.62x51, you start thinking about a new caliber making “puncture” wounds. And I even checked all the different varieties of the 7.62x51 and how it effects a bullet proof vest. 

So, know for me that it is the lighter full metal jacket and the idea of actually making a flesh wound.

Springfield wants to get this out of here. Okay, so cool.

And I bought 4 straight from the manufacturer, 

1. The M1A described above, with a standard stock in diamond cut raw black walnut

2. An M1A with no modification, and a standard stock in diamond cut raw Beech

3. A standard M1A

4. A standard M14

And along with my .40 cal XDM, those are my Springfields. And there is no thumbhole stock.

https://www.reuters.com/world/us/us-house-panel-seeks-details-five-gunmakers-after-shootings-2022-05-27/

[epigen-papers]

Reversible RNA Modification N1-methyladenosine (m1A) in mRNA and tRNA.

Pubmed: http://dlvr.it/QXZQTh

[vernicle]

Proven Reliability of the Zongshen Cyclone RX3

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TheCyclone RX3 dispersed by CSC Bikes was launched with an inaugural group experience via Baja Mexico in April 2015. This experience took fifteen journey bikers on a 1,seven-hundred-mile spherical excursion via the Baja peninsula and back to the CSC places of work in Azusa, California. In overall, the fifteen new Cyclone RX3 bikes accrued twenty five,500 miles with only a few slight challenges.

This first advertising experience was adopted by the "Western Journey Tour" in July 2015. Here 10 riders from Columbia, China, and the U.S. accrued fifty,000 miles overall on 10 new bikes. This epic experience encouraged the e-book, 5,000 Miles at eight,000 RPM's by Joe Berk, the spokesman for CSC. Once more, there were being no main mechanical challenges with the new CSC Cyclone RX3 bikes that were being ridden tricky for eighteen days at speeds up to 75 mph, and often about 400 miles per working day. The tour riders (and the devices) encountered freezing temps to about one hundred degrees, and elevations from sea amount to nine,000 feet at the Continental Divide.

These rides proved conclusively the longevity of the RX3 journey bicycle. But the rides also pointed out some advancements that would be demanded on subsequent shipments of the CSC Cyclone RX3 which includes upgrades to the regular 12-volt batteries. The rides even more helped to finalize the routine maintenance agenda suggested for the North American cycle proprietors.

But the RX3 motorbike was confirmed ahead of it was at any time readily available for sale in North The united states. The bicycle has been bought in China for quite a few yrs, and is also bought in a lot of other nations around the world below numerous manufacturer names. For example, the identical simple bicycle is bought in Belarus as the M1nsk TRX300i and in Central The united states as the AKT Moto TT250. In reality, Zongshen's solitary greatest motorbike current market is in Columbia. Motorcycle product sales exceed seven-hundred,000 models per year in Columbia - compared to fewer than 500,000 overall motorbike product sales in the United States.

It is also intriguing that the most preferred bikes in Columbia are only 100cc to 125cc. A 250cc motorbike is viewed as massive! The best marketing device is a 100cc motorbike manufactured in India. The Zongshen/AKT Moto model bought in Columbia is named the TT250 and is viewed as to be a significant-top quality update and exudes status.

What is a M1nsk?

The M1nsk motorbike corporation originated in 1951 when the USSR took control of German DKW Bikes as component of WWII reparations. The corporation relocated to Moscow and crafted bikes below the M1A manufacturer. Finally, the factory moved to Belarus in which they crafted bikes below the Minsk manufacturer. From the early yrs the Minsk manufacturer has been actively included in motorsport, primarily in motocross and circuit racing. Minsk-Pamir (1969) and Brest-Vladivostok (1971) rallies grew to become iconic milestones in the Minsk manufacturer historical past. Dependent on this track record, Minsk bikes grew to become acknowledged for their longevity and were being exported to other European nations around the world.

In 2007, the corporation grew to become personal and commenced using the manufacturer name M1nsk. Today the M1nsk corporation makes snowmobiles, ATV's, scooters, dirt bikes, and retro styled bikes dependent on their aged 1950's model designs.

How does the M1nsk TRX300i in shape in? M1nsk does not manufacture the TRX300i journey motorbike. Alternatively, the TRX300i is a rebranded Chinese motorbike, the Zongshen RX3. Zongshen is a fashionable company, launched in 1992, that builds about 1,000,000 (sure, that is 1 MILLION) engines and bikes per year. Numerous of these styles are now bought around the globe below a variety of brand names by learn distributors.

The U.S. and North American distributor is CSC Bikes, and sells the CSC Cyclone RX3 direct to the community. To join the past with the present, M1nsk entered a few stock TRX300i bikes in the 2013 Brest-Dushanbe rally with overall duration of six,200 miles (10,000 km.) The rally traveled via the rugged terrain of Belarus, Russia, Kazakhstan, Kyrgyzstan and Tajikistan and all a few bikes accomplished the journey. This rally was earlier covered in an short article by ADVPulse in February 2014.

Be aware: this "rally" is not the Baja 1000 or the Dakar Rally. Believe far more along the strains of Ewan and Charley traversing the rough countryside of the identical area in Prolonged Way Close to. A video of the rally (in Russian) can be seen at the Minsk Bikes YouTube channel.

Once more, it was an impressive endurance milestone that the a few stock bikes from Zongshen covered 30,000 km cumulative length with no mechanical challenges.

The CSC Cyclone RX3: Proven Reliability

The M1nsk TRX300i is practically equivalent to the Cyclone RX3 marketed via CSC Bikes. Even so, one more exceptional attribute of Zongshen is that every single national distributor has the ability to make modifications in the ultimate specs of the motorbike to comply with restrictions for their continent and the calls for of their focus on prospective buyers. In his e-book, 5,000 Miles at eight,000 RPM's, Joe Berk outlines how CSC produced modifications to the wheel size, alternator potential, wiring harness, battery and other components ahead of finalizing the model that would become the Cyclone RX3 for sale in North The united states. In addition, CSC Bikes has also assembled a comprehensive catalog of aftermarket areas, add-ons, selections, and exclusive editions to accommodate the specifications of journey riders in the U.S. and Canada.

The internet end result is that the North American Cyclone RX3 is even Greater than the initial Zongshen model. Keep in mind that tens of countless numbers of these initial styles have currently been confirmed in Asia, Central The united states and Europe. (The Initial purchase of the AKT Moto model by the Columbian distributor was six,000 bikes!) Since 1992, Zongshen has manufactured tens of Thousands and thousands of engines in a lot of dimensions. The condition-of-the-artwork manufacturing facility assessments Each and every of these engines ahead of assembly into a comprehensive motorbike, and then Each and every of these comprehensive bikes is dyno tested for top quality control.

Although journey bikes are a developing recreation market right here in North The united states, Zongshen bikes have acquired a strong track record for top quality and longevity in the rest of the planet in which bikes are the most important usually means of transportation and integral to commerce. These motorbike proprietors need economical operation and dependability. In some of these nations around the world, the average annual earnings is fewer than the price tag of a established of the extravagant aluminum panniers discovered on a lot of European "journey bikes"!

On prime of this, CSC Bikes has produced the commitment to constantly refine the base bicycle and the variety of areas and add-ons for sale to North American riders. CSC Bikes is which includes a total routine maintenance guide with every single Cyclone RX3 and is also generating a detailed catalog of tutorials that go over all features of regime routine maintenance. At last, CSC is standing behind the Cyclone RX3 with a guarantee that handles areas and labor for one year and all areas for two yrs with Unrestricted mileage.

Is the Cyclone RX3 the proper journey bicycle for you? That is a very individual selection given that everyone's specifications are unique. Even so, the dependability of the Cyclone RX3 is with no question. Not only has CSC Bikes subjected the motorbike to torture assessments in true existence touring, but tens of countless numbers of journey riders about the planet have also confirmed its reliability. There is a developing and devoted "cult of the Zong" related to this motorbike mainly because it not only works but it outperforms bikes that price tag two to 5 situations as substantially for a comparable fully-equipped touring deal. Even more proof of the dependability of the Cyclone RX3 is that two national motorbike rental and tour corporations now offer you this model for lease and in tour deals.

Now readily available: the CSC RX3-P Law enforcement motorbike - a specifically-equipped variation of the Cyclone RX3 Journey model that has been employed (and abused) by law enforcement in Asia! Stay tuned to the CSC Website for far more info.

References:

ADVPulse, 02112014

CSC Bikes (cscmotorcycles.com)

[ad_2] Supply by Randy Reek

from Viral News Around The World - Feed http://ift.tt/2rPS1Lf via IFTTT

[rnomics]

RMDGCN: Prediction of #RNA methylation and disease associations based on graph convolutional network with attention mechanism

by Lian Liu, Yumeng Zhou, Xiujuan Lei #RNA modification is a post transcriptional modification that occurs in all organisms and plays a crucial role in the stages of #RNA life, closely related to many life processes. As one of the newly discovered modifications, N1-methyladenosine (m1A) plays an important role in gene expression regulation, closely related to the occurrence and development of diseases. However, due to the low abundance of m1A, verifying the associations between m1As and diseases through wet experiments requires a great quantity of manpower and resources. In this study, we proposed a computational method for predicting the associations of #RNA methylation and disease based on graph convolutional network (RMDGCN) with attention mechanism. We build an adjacency matrix through the collected m1As and diseases associations, and use positive-unlabeled learning to increase the number of positive samples. By extracting the features of m1As and diseases, a heterogeneous network is constructed, and a GCN with attention mechanism is adopted to predict the associations between m1As and diseases. The experimental results indicate that under a 5-fold cross validation, RMDGCN is superior to other methods (AUC = 0.9892 and AUPR = 0.8682). In addition, case studies indicate that RMDGCN can predict the relationships between unknown m1As and diseases. In summary, RMDGCN is an effective method for predicting the associations between m1As and diseases. https://journals.plos.org/ploscompbiol/article?id=10.1371%2Fjournal.pcbi.1011677&utm_source=dlvr.it&utm_medium=tumblr

[rnomics]

A novel scoring model based on #RNA modification "writers" can predict the prognosis and guide immunotherapy in gastric #cancer

Although extensive research has been carried out on the epigenetic regulation of single #RNA modifications in gastric #cancer, little is known regarding the crosstalk of four major #RNA adenosine modifications, namely, m6A, m1A, alternative polyadenylation and adenosine-to-inosine #RNA editing. By analyzing 26 #RNA modification "writers" in 1750 gastric #cancer samples, we creatively constructed a scoring model called the "Writers" of the #RNA Modification Score (WRM_Score), which was able to quantify... https://pubmed.ncbi.nlm.nih.gov/37188931/?utm_source=dlvr.it&utm_medium=tumblr&utm_campaign=None&utm_content=1RYYbE7j9SUSBe_aHniaI_J1MQIFIBbfLuFxoWdLNMNDzVVIWF&fc=None&ff=20230518031416&v=2.17.9.post6%2086293ac

[rnomics]

Ontogenic #mRNA expression of #RNA modification writers, erasers, and readers in mouse liver

by Liming Chen, Pei Wang, Raman Bahal, José E. Manautou, Xiao-bo Zhong #RNA modifications are recently emerged epigenetic modifications. These diverse #RNA modifications have been shown to regulate multiple biological processes, including development. #RNA modifications are dynamically controlled by the “writers, erasers, and readers”, where #RNA modifying proteins are able to add, remove, and recognize specific chemical modification groups on #RNAs. However, little is known about the ontogenic expression of these #RNA modifying proteins in various organs, such as liver. In the present study, the hepatic #mRNA expression of selected #RNA modifying proteins involve in m6A, m1A, m5C, hm5C, m7G, and Ψ modifications was analyzed using the #RNA-seq technique. Liver samples were collected from male C57BL/6 mice at several ages from prenatal through neonatal, infant, child to young adult. Results showed that most of the #RNA modifying proteins were highly expressed in prenatal mouse liver with a dramatic drop at birth. After birth, most of the #RNA modifying proteins showed a downregulation trend during liver maturation. Moreover, the #RNA modifying proteins that belong to the same enzyme family were expressed at different abundances at the same ages in mouse liver. In conclusion, this study unveils that the #mRNA expression of #RNA modifying proteins follows specific ontogenic expression patterns in mice liver during maturation. These data indicated that the changes in expression of #RNA modifying proteins might have a potential role to regulate gene expression in liver through alteration of #RNA modification status. http://bit.ly/2ZF24C5