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DNA Replication Enzymes (In Prokaryotes)

DNA Replication Enzymes (In Prokaryotes)

Replication in E. coli requires not just a single DNA polymerase but 20 or more different enzymes and proteins, each performing a specific task. The entire complex has been termed the DNA replicate system or Replisome. Core Proteins at the Replication Fork. 1. Helicases – DnaB Open the double-stranded DNA with consuming ATP. Hexamer (6 subunits present) Opening process with the assistance of…

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Biomedical Nanotechnology

Nanoparticles are any material having at least one of its dimensions in the range of 1-100nm. Nano – derived from a Greek word Nanos meaning DWARF or small. Norio Taniguchi 1974 — coined the term nanotechnology. A nanometre is one-billionth of a meter. A simple exampleThe population of India is one billion or 100 crores. Each Indian you or I is nano in comparison with the total population of…

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Pedigree Analysis

Pedigree Analysis A family tree in which we observe the passing of a particular trait from one generation to another. Autosomal dominant inheritance• An affected person usually has at least one affected parent (usually).• It affects either sex.• It is transmitted by either sex.• A child with one affected and one unaffected parent has a 50% chance of being affected. Autosomal recessive…

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[v-vibesblog]

Basic Concept of CRISPR-CAS9

Basic Concept of CRISPR-CAS9

This is a post originally published as part of The Jackson Laboratory. Enroll in this program and get a certificate, and start your carrier. CRISPR/Cas9, or simply “CRISPR”, is one of the most important developments in genome engineering in recent years. It permits highly efficient and precise genome-editing using reagents that are both simple to design and inexpensive to generate. Basic…