dearmeleddy

[dearmeleddy]

Im a cool & independent & successfully eat alone at cafe hosp alone. Fine and no one to interrupt. Wanna cry so much, but no time. There's no need to cry. Im a big girl. Head held high. Pretty cute, lovely girl. Yess that's you be proud of yourself. Love you.

Xoxo me

[dearmeleddy]

Dear me,

A journey in life is an unkown mystery that makes you wonder in your life.

Enjoy and go through with smile. Life is so much more better with that.

Be gentle to yourself.

Sincerely, myself love

[dearmeleddy]

Keep doing what you're doing, get together, and focus on your journey. You may be taking a different path, but trust yourself

[dearmeleddy]

Ugh, i felt so disgusted with myself. I had a crush on someone h******. Now, i don't like that person anymore and turn off. It's time to move on. Ewww, never in my life, i ever felt like this. I never thought that person was m** 🤢 i just can't believe myself.

[dearmeleddy]

I love my salad so much 😋 so easy to make, suitable for student that's want to eat healthy and quick to serve.

Only need:

1. greek yoghurt

2. Apple,cucumber, green. (amount based on how much you want)

[dearmeleddy]

Learn and try to love harder for what i am doing right now. But I was born into wanting to be a great artist. Living aesthetically doing what i love, such as drawing. Maybe if i focus on practising on drawing, maybe i become more good in what i am today.

[dearmeleddy]

I want to cry. Life is so much harder than it seems to be. It's a bittersweet journey that i need to go through. I need to keep my faith stronger and not be scared.

[dearmeleddy]

I realized that I didn't feel that sad knowing I've treated very badly by people around me. I've come to understand that not everyone is happy and fond with me. However, I am willing to tolerate it cause I've been used to it. It is still unpleasant to me to experience the treatment. It did hurt my feelings, but it's a very hard and cruel world we live in today. I just need to get through it. I'm glad i didn't cry. Well, it's gonna bottle up again, and when the times come, it will burst out, and im gonna let it all out and cry again like I'm always did.

I have many things I want to do, but i don't want to be rude. Sometimes, i don't realise that my actions came across as rude to other people, which resulted in me being a bit timid afterwards. I hate that feeling. Life is so hard sometimes. I want to be confident so much. I will, and I am. In the next 5 years, I will be someone who is totally different from what I am today.

I will become someone like an excellent speaker and have fantastic social and lab skills as well as very beautiful and confident, that i am no more of the person who is less skilled in handling laboratory equipment and instrument. I work with an extraordinary team and company that is well known in the research world.

I promise that i won't dissapoining myself and my family. I want to prove that i can be the person that I dream to be.

[dearmeleddy]

Quick update:-

Tuesday (10/9/2024) - preparation of calibration curve & qc & reconstitute sample till transfer into vial & injection inside the uplc system vitamin d.

Wednesday (11/9/2024) - understanding, reading & analysing the peak, data in Excel with formulated table provided (retention time, area, height, graph) result from the injection of qc, cc, samples. Visited the mycero lab at hukm, level 12, for uhplc for vitamin A & E.

Thursday - should be on method validation of uplc (not yet known)

[dearmeleddy]

9/9/24 _ monday

Today, i am able to do a sample step in preparing the sample from the serum transfer to the drying step of hexane extract of vitamin D.

1. retrieved sample (600 ul) from biobank.

2. transfer serum (500 ul) into a borosilicate tube. *Prewet tip with serum before transfer*

3. add Internal Standard (15 ml) *prewet tip with IS before take volume, tip at wall of tube*

Vortex

4. Add methanol for deproteinazation (500 ul)non-critical

5. Add n-hexane (1000 ul) for extracting the metabolite in the serum.

Vortex (30 sec), centrifuge (10 mins), transfer hexane mixs -> new tube

6. Transfer upper layer (1 ml long blue tips)

7. Add hexane (1000 ul)

Vortex (30 sec) (centrifuge 10 mins)

8. Transfer for 2nd time to same tube. (Long blue pipette tip + yellow small pipette tip)

9. Drying in labconco centrivap concentrator (20 mins)

* Should the total volume in the tube (2 ml) (hexane mixs contain vitamin D)

* If the mixture is yellow, possible of carotenoids inside, not only vitamin D.

[dearmeleddy]

Outside my apartment #dark #cyberpunk #cinematography #camera

[dearmeleddy]

Drawing while listening to stephanie soo video is a pleasingly aesthetic and soothing.

[dearmeleddy]

Missing my cats so much. Can't wait to see them next week. My cats are my happy pills. Urggh.... need to recharge my energy.

[dearmeleddy]

What a small world we live in. I just came across a professor from a medicine's faculty at this one hospital that i also went to. She was a participant in the qpcr workshop that i attended.

I never thought that i actually met her outside from the workshop. But, well, life is so much more mystery. I came across her on my way to get back to my institute. Fyi, my institute building is across from her institute building. Basically, I need to go through her building before i can reach my building.

The story starts when I attend an event at the auditorium of the hospital, where there are a variety of stalls that sell different types of food. So, I bought some cuisine, then walked through the pedestrian crossing. While i was walking, i noticed that there was a middle-aged woman walking in front of me with bags of food. I realized that she must have gone to buy food at the event, too. Nothing crossed my mind that the person walking in front is the same professor who attended the qpcr workshop.

She was trying to get to level 15 while i was trying to get to level 1, which is the main lobby. But, i mistakenly entered the lift that was going up. The time when i realized my mistake, it had been late cause the lift had already closed and went up.

Finally, i looked at the woman and noticed that she was the professor! She also noticed, and we laughed, because we never thought that we would meet each other in these circumstances.

That's all that happened today. I am just eager to share my story. Nothing special, but it is kind of humorous.

[dearmeleddy]

I realized today that everyone has they own problem that they need to face and solve.

When they share their problem, i realize that i should be more grateful for everything that happen in my life.

Im thankful because there would always be someone who came to my life to teach me and help me on my way to my journey.

Be grateful always. It doesn't matter if it is bad or good. Life is full of roller coster.

There's a phrase that says, "There's would be a rainbow 🌈 after the rain."

So, everything that happened is a lesson for you to keep on polishing yourself to become a better version of you.

[dearmeleddy]

Yesterday, i made the best decision on just doing it.

Well, my sister asked me if i wanted to drive the car, then, instead of overthinking and fearing if bad things were gonna happen, i decided to just drive the car. And it's turned out to be better than what i expected.

We managed to arrive home safely. I successfully drove and parked the car.

This made me realize that overthinking kept me from achieving or getting my goal done.

If you want to do something, just do it. It didn't hurt you. On the contrary, you had the opportunity to experience and complete your dream/goal.

Therefore, moral of the story, JUST DO IT!

Life is much more exciting when you just go for it.

[dearmeleddy]

Hiii, this is my first time in tumblr. Just want to randomly post anything i like. I wanna make it like my small blog/diary.