Journal of World’s Poultry Science (JWPS) is an international peer-reviewed open-access journal that publishes original research reports, case reports, and reviews on poultry diets and nutrition. The JWPS is currently dedicated to bimonthly publishing articles in the subject areas of the poultry industry, husbandry and management, environment and welfare, exotic and wild birds, biochemistry and cellular biology, layer and quail management, nutrition and feeding, physiology, genetics, reproduction and hatching, technology, processing and food safety, behavior, environment and welfare, pharmacology, hematology, oncology, molecular biology, zoonoses diseases, diet formula, performance, carcass traits, survival, and probiotics. There is no Article Processing Charge (APC) in this journal.
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Carcass yield and meat cuts are traits of meat quality that determine consumers’ preferences. The present study investigated the effects of feeding ash-treated African locust bean hull meal partially replacing palm kernel meal on broiler chicken carcass and gut organs.
Materials and methods: One hundred and twenty Arbor Acre broiler chickens were randomly distributed on an equal weight basis into three groups of 40 broilers each, and each group was replicated four times with 10 chickens per replicate in a completely randomized design. Each group was assigned to three experimental diets in which Ash-treated African locust bean pulp replaced palm kernel cake at 0 % (T1, Control), 25% (T2), and 50% (T3). At the end of the feeding trial, which lasted for 42 days, a chicken each was randomly selected per replicate per treatment group (twelve in total), and final live weight, final feed intake, and feed conversion ratio were measured before slaughtering.
Available at :https://jwps.rovedar.com/index.php/JWPS/article/view/64
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Pitalah ducks are one of Indonesia's local genetic resources that can be utilized to develop quality livestock products. The present study aimed to identify the diversity of the Myostatin gene (MSTN|HaeIII) exon-3 in Pitalah ducks, a local duck breed from West Sumatra, Indonesia.
Materials and methods: The present study utilized 40 samples of DNA from the blood of Pitalah ducks (11 males and 29 females, with an average body weight of 1,450 g and an average age of 12 months) that were intensively reared at the Andalas University animal husbandry unit in Indonesia. The DNA from blood samples was isolated using the genomic DNA Extraction Kit. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used as the method. The isolated DNA was then amplified using a primer that produced a 438 bp fragment of the MSTN gene. The amplification product was restricted using the HaeIII enzyme, which recognizes the cutting site at GG↓CC.
Results: Based on the findings of 40 samples, 65% of homozygous truncated genotypes (+/+), 35% heterozygous genotypes (+/-), and no homozygous untruncated genotypes (-/-) were found. Based on the current results, two genotypes were identified. The current findings indicated genetic diversity, as the frequency of each allele exceeds 1%. The allele frequency (+) was 0.825, the allele (-) was 0.175, and the observed heterozygosity was greater than the expected heterozygosity.
Conclusion: The allele frequency of the MSTN|HaeIII exon-3 gene in Pitalah ducks is polymorphic (diverse) within the Pitalah duck population. Genotype frequencies of the MSTN|HaeIII exon-3 gene are in Hardy-Weinberg equilibrium. https://www.instagram.com/p/DJybX3xIvnb/?igsh=bXl0cDI2cmlqdnJn

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Marek’s disease (MD) is a major threat to the poultry industry worldwide and significantly affects production and economic stability. In this context, an outbreak in a commercial poultry farm in Naththandiya, Sri Lanka, served as a crucial alarm to avian health management. The clinical signs found during this outbreak not only highlight the virulence of MD virus but also raise questions regarding the effectiveness of the current control measures.
Case report: The outbreak was found in a commercial poultry farm with 4500 Brown Leghorn female chickens including 2500 of 14 weeks old chickens, and 2000 of 6 weeks old chickens in Naththandiya, which was characterized by marked mortalities of 15-20 chickens/day in elder chickens and 35-40 chickens/day in 6 weeks old chickens. The older chickens have been vaccinated with the oral Infectious Bursal Disease (IBD) vaccine at 3 weeks and 11 weeks of age and also with Fowl Pox vaccine at 8 weeks of age. The younger chickens have been given only IBD vaccine at 3 weeks of age. The outbreak showed a wide range of clinical signs, including swollen and closed eyes, nasal discharge, respiratory distress, and anorexia. The post-mortem examination revealed pathognomonic lesions such as grey-white foci of neoplastic tissue in the liver, lungs, and kidneys, enlargement of the sciatic nerve with loss of cross striations, visceral organ enlargement such as liver, kidney, and spleen which indicates the presence of MD virus along with secondary infections caused by Escherichia coli (E. coli). Histopathological examination and bacterial culture confirmed the MD and E. coli infections respectively.
Conclusion: This outbreak highlights the importance of vigilant surveillance, proactive management, and continuous review of vaccination protocols to control the transmission of Marek’s disease effectively. https://www.instagram.com/p/DFkt4zgo10x/?igsh=MWpmejYzdHN5MGlhMQ==

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Marek’s disease (MD) is a major threat to the poultry industry worldwide and significantly affects production and economic stability. In this context, an outbreak in a commercial poultry farm in Naththandiya, Sri Lanka, served as a crucial alarm to avian health management. The clinical signs found during this outbreak not only highlight the virulence of MD virus but also raise questions regarding the effectiveness of the current control measures.
Case report: The outbreak was found in a commercial poultry farm with 4500 Brown Leghorn female chickens including 2500 of 14 weeks old chickens, and 2000 of 6 weeks old chickens in Naththandiya, which was characterized by marked mortalities of 15-20 chickens/day in elder chickens and 35-40 chickens/day in 6 weeks old chickens. The older chickens have been vaccinated with the oral Infectious Bursal Disease (IBD) vaccine at 3 weeks and 11 weeks of age and also with Fowl Pox vaccine at 8 weeks of age. The younger chickens have been given only IBD vaccine at 3 weeks of age. The outbreak showed a wide range of clinical signs, including swollen and closed eyes, nasal discharge, respiratory distress, and anorexia. The post-mortem examination revealed pathognomonic lesions such as grey-white foci of neoplastic tissue in the liver, lungs, and kidneys, enlargement of the sciatic nerve with loss of cross striations, visceral organ enlargement such as liver, kidney, and spleen which indicates the presence of MD virus along with secondary infections caused by Escherichia coli (E. coli). Histopathological examination and bacterial culture confirmed the MD and E. coli infections respectively.
Conclusion: This outbreak highlights the importance of vigilant surveillance, proactive management, and continuous review of vaccination protocols to control the transmission of Marek’s disease effectively.
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The use of antibiotics in poultry feed is becoming more prohibited globally, it is important to consider the potential of natural feed additives as antibiotic substitutes to support the development and operation of chickens' immune systems. The study aimed to assess the effect of probiotic and synbiotic supplementation in broiler feed on the post-hatch development of broiler spleen.
Available at:https://jwps.rovedar.com/index.php/JWPS/article/view/42
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One hundred twenty White Leghorn layers at 32 weeks of age were randomly allocated to four treatments each replicated three times with ten layers and one cock per replication and managed on a deep litter system for 70 days. The treatments were control group without any addition of feed additives (T1), basal diet supplemented with 4 milliliters of onion extract in water (T2), basal diet supplemented with 4 milliliters of rosemary extract in water (T3), and basal diet supplemented with 4 milliliters of onion and rosemary extract mixture in water (T4). The data on performance parameters such as feed intake, body weight change body weight gain, feed conversion ratio, egg production, egg mass, and serum biochemistry and hematology were evaluated.
Results: The daily feed intake of chickens in T2 was significantly higher than the feed intake of chickens in T1, T3, and T4. There was no significant difference in packed cell volume percentage but there was a significant difference in total serum cholesterol in T1 compared to T4. Layers supplemented with onion extract had lower blood total cholesterol levels compared to other groups. Results revealed that the use of onion extract (T2) in drinking water recorded significantly higher daily feed intake, body weight change, and gain compared to other groups. Layers fed on onion blended with rosemary extract in drinking water recorded the highest feed conversion compared to T1, T2, and T3 treatments.
Conclusion: The use of rosemary, onion extracts, and their mixtures in drinking water significantly influenced most of the studied hematological and serum biochemical parameters of White Leghorn layers except packed cell volume and serum albumin.
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The reduced susceptibility of microorganisms to synthetic preservatives and consumer's demand for high-quality, and minimally processed green-label foods urged researchers to focus more on natural preservatives. This study aimed to evaluate the in vitro and in sacco antifungal activities of Ageratum conyzoides leaves essential oil (EO) against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Penicillium spp. in poultry feed.
Materials and methods: The poultry feed was stored for 30 days. The dilution plate method was then used to isolate the fungi present in the feed. The leaves of Ageratum conyzoides were collected and their EO was extracted using Steam distillations. The in vitro antifungal activity of EO (2.3; 3.4; 4.0; 4.5; 5.1 and 5.7; [µl/cm2]) was tested using the modified method, against the above fungi species during a 4-week of research. In sacco antifungal Screening of EO (5.7; 5.1; 4.5 and 4.0μl/Kg of feed) was done on poultry feed and kept for 10, 20, and 30 days.
Results: The most frequently isolated fungi in poultry feed was Penicillium spp. (84.07%). The smallest in vitro colony diameters were recorded by Aspergillus niger with 4µl of EO. The highest level of fungi growth reductions was recorded at 2.3µl of EO. Following 20 days of storage, 4.0µl of EO/kg of feed was shown to completely (100%) inhibit Aspergillus niger and Aspergillus terreus in the Sacco research. At 30 days of storage, there was no contamination in poultry feed especially in aspects of Aspergillus flavus, Aspergillus niger, and Aspergillus terreus. Penicillium sp. contamination in poultry feed decreased as the EO concentration increased.
Conclusion: The results of the current study indicated that Ageratum conyzoides leaves essential oil at the level of 4.0µl of EO/kg of feed had the highest prevention effects on fungus growth.
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Poultry feed management requires novel dietary additives to enhance chicken performance and manage feed availability. The present study investigated the effects of dietary supplementation of Carica papaya leaf meal (CPL) and fermented fish waste (FFW) in water on different growth indicators, such as body weight, carcass yield, feed intake, and conversion rate of Bovans brown male broiler chickens.
Materials and methods: A randomized complete block design with a 2x4 factorial arrangement was used to study the effect of the combination supplementation of the 10% CPL with FW at 0%, 5%, 10%, and 15%. Both additives combination treatment was replicated four times with five broiler chicks per replication, a total of 160-day-old male Bovans brown broilers with 39-40g body weight were used in the current study. The duration of the study was 20 days (from the 16th to 35th days of age).
Results: Studies have revealed that CPL negatively impacted the final body weight, whereas FFW positively influenced feed intake, final weight, and weight gain. Moreover, 15% of FFW supplementation resulted in noteworthy increases in drumstick and thigh weights compared to the control group, with significant interactions identified between CPL and FFW for breast, wing, and back weights. However, neither CPL nor FFW had an impact on the weights of edible visceral organs. The palatability of meat quality assessment showed no significant difference among the treatments. Adding CPL and FFW increased weights and profits, with the best rate of intake observed in broilers given the standard diet plus 15% of FFW supplementation.
Conclusion: These results indicated that using CPL and FFW at 10% and 15%, respectively was a financially feasible approach to improve broiler development, carcass quality, and profitability while capitalizing on nutritional advantages and reducing environmental waste.
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Soursop leaves are rich in various molecules, including total phenols, terpenes, and steroids, which possess a range of pharmacological properties that can be utilized in animal production to enhance both growth and reproduction of animals. The present study aimed to evaluate the effect of incorporating soursop (Annona muricata; A. muricata) leaves powder into feed on the reproductive performance of Japanese quail.
Available at:https://jwps.rovedar.com/index.php/JWPS/article/view/39
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Indexing and Archiving
JWPS is indexed in WorldCat (OCLC)
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Indexing and Archiving
JWPS is indexed in OpenAIRE
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The ban on the use of antibiotics as feed additives in livestock farming has led to the development of alternative products. This study aimed to evaluate the effects of combining phytobiotic (Dichrostachys glomerata) with a probiotic (Thepax®) on the growth performance of Japanese quails.
Results: The findings indicated that quails fed on 0.6 ml probiotic / L of drinking water had the highest weight gain and the lowest feed conversion ratio. Combining phytobiotic with probiotics at 0.4 ml and 0.5 ml improved the apparent digestibility of crude protein and organic matter. Although the treatment did not significantly affect the carcass yields, the relative weight of the liver and pancreas were lowest with 0.4 ml probiotic. Haemato-biochemical parameters increased with higher levels of probiotics combined with 4 g of phytobiotics in the feed, leading to an increase in serum content in ALT and creatinine. Combining 4 g of the phytobiotic with 0.6 ml of probiotic resulted in a decrease in pathogens (Salmonella and E. coli) count in the gastrointestinal tract of quails.
Conclusion: The combination of Dichrostachys glomerata (4g /kg feed) to a probiotic (0.6ml /L drinking water) improved feed conversion ratio and live weight gain while reducing the pathogenic microorganisms in the gastrointestinal tract of Japanese quails.
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Phytate poses a significant challenge in poultry nutrition due to its anti-nutritional properties. Phytate is one of the antinutritional factors that is considered harmful to broilers. This study aimed to evaluate the impact of incorporating phytase enzymes on the health of chickens and their ability to digest nutrients from the baobab seed meal.
Available at:https://jwps.rovedar.com/index.php/JWPS/article/view/29
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JWPS is indexed in AGRIS .
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Inefficient poultry production has been a serious challenge due to poor performance and scarcity of feed resources, which necessitate the exploration of alternatives. This study was conducted to assess the effect of Saccharomyces cerevisiae (SC) as a supplemental protein and growth promoter in different cereal-based diets on growth performance, serum biochemistry, characteristics of digesta, and nutrient digestibility of broiler chickens.
Materials and methods: A total of 324 day-old broiler chickens, comprising of mix sexes with an average weight of 45.36 ± 0.73, were randomly assigned to nine treatments, each consisting of three replicates with 12 birds per replicate. Three diets were formulated, each incorporating maize, sorghum, and wheat. The diets varied in the SC inclusion at levels of 0%, 5%, and 0.2% oxytetracycline (used as a growth promoter). The inclusion of oxytetracycline was to simulate the practice of in-feed antibiotics growth promoter, whereas yeast served as supplemental protein and growth promoter. Titanium dioxide was included in the feed at 0.2% on day 21 of the experiment to help estimate nutrient digestibility.
Results: Weight gain and feed conversion ratio were significantly lower in the groups fed sorghum with and without yeast. The groups fed maize with SC and oxytetracycline had the lowest blood glucose. Maize and sorghum without SC had the highest pH values. Protein digestibility was the lowest in the group fed sorghum with SC and wheat without SC.
Conclusion: Individual cereal diets performed competitively, supplementation of SC in different cereal-based diets did not influence their contribution and performance of the chickens. The inclusion of 5% SC reduced protein digestibility. Oxytetracycline yeast as a supplemental protein and growth promoter did not improve the performance of the chicks.
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The global ban on antibiotics as a feed additive, prompted by concerns over microbial resistance and the accumulation of antibiotic residues in animal products, has urged researchers to explore natural alternatives. These alternatives modulate the gut microbiota and enhance animal production performance. This study aimed to assess the impact of Allium sativum on the production performance of broiler chickens.
Materials and methods: A total of 280 day-old broiler chicks were examined for 42 days. After being sexed, they were randomly allocated into five groups, each consisting of 14 chicks, with four replications. The control group was fed on a ration without additives (R0-). The treatment groups consisted of a control diet supplemented with 1 g of antibiotic Doxycycline® per kg of basal diet and per liter of drinking water (R0+) and 5 g garlic per kg of basal feed and also per liter of drinking water (RAs), respectively. To facilitate the oral administration, the solution was absorbed by charcoal from the fruit stones of Canarium schweinfurthii at a rate of 100 g and 100 ml per kg of diet and per liter of drinking water, respectively. The investigated parameters included growth performance, microbial flora, markers of kidney, and liver function, and feed digestibility.
Results: The results revealed Allium sativum significantly decreased feed intake, feed conversion ratio, serum aspartate aminotransferase, alanine aminotransferase, and urea levels regardless of the administration mode in broiler chickens, compared to the negative control. When administered through both feed and drinking water, garlic significantly increased live weight, weight gain, and lactic acid bacteria count, compared to the negative control. Feeding garlic to broilers had insignificantly affected the digestibility of feed components irrespective of the administration mode.
Available at:https://jwps.rovedar.com/index.php/JWPS/article/view/25
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The major histocompatibility complex (MHC) of chicken is highly polymorphic, and it is linked to several disease resistance or susceptibility traits. Therefore, the current study aimed to analyze the genetic diversity in the MHC region of Nigerien local chicken (Dourgou, Goggori, Kolonto, Tchagara, Gouzou-gouzou, and Popular) using a high polymorphic microsatellite marker named LEI0258 to determine the diversity of chickens kept at the four agroecological zones in Niger.
Materials and methods: A total of 601 chickens from six local Nigerien chickens were sampled. By capillary electrophoresis using LEI0258 marker, 403 samples with different fragment sizes were randomly chosen and sequenced.
Results: The findings indicated 80 different alleles ranging in size from 181 to 474 bp. A total of 22 new alleles and 39 private alleles (that existed in only one breed) were detected. The alleles 309, 295, and 193 were the most predominant in the Nigerien local chicken population. Nine polymorphisms were observed along the LEI0258 sequence, including three in the upstream (one indel and two Single Nucleotide Polymorphism [SNP]), one in the repeat region at the last R12 (SNP), and five in the downstream (two indels and three SNPs).
Conclusion: The chickens are not clustering according to their agroecological zone of origin. They are randomly distributed across the four investigated agroecological zones. The information found in this study is invaluable in breeding and conservation programs associated with several disease resistance or susceptibility traits.
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