#Bioorthogonal

[yashmedica]

Unnatural Amino Acids: Beyond the Basics - Unlocking New Research Frontiers

Unnatural amino acids are revolutionizing research and bioengineering. Explore their applications in protein engineering, biomaterial design, and drug discovery. Discover the top players in the market and the exciting future of unnatural amino acids

The Unnatural Amino Acids Market: Pushing Boundaries in Bioengineering The unnatural amino acids market is a rapidly evolving field with vast potential in various scientific and technological applications. Unnatural amino acids are molecules structurally similar to the 20 naturally occurring amino acids that form the building blocks of proteins. However, unnatural amino acids possess unique…

[s-lorelei]

Things women created and discovered!

All men

Francium (elemental)

Torpedoe radio guidance/navigation systems

Dishwasher

GPS

Wi-fi

Structure of the Milky Way

Kevlar

The Earth's inner core

Aciclovir - an antiviral drug used for the treatment of herpes simplex virus infections, chickenpox, and shingles

Azathioprine - an Immunosuppressive drug used in rheumatoid arthritis, Crohn's disease, ulcerative colitis, and in kidney transplants to prevent rejection

Flossie Wong-Staal was the first scientist to clone HIV and map its genes.

Pyrimethamine was initially developed by Nobel Prize winning scientist Gertrude Elion as a treatment for malaria.

Disposable diapers

Child carriers

Vaccine for whooping cough

The galaxy rotation problem - important to the discovery of dark matter

Radio astronomy - Type I and Type III solar radio bursts

That stars are primarily composed of hydrogen and helium

The new outer arm of the Milky Way - In 2004, astrophysicist and radio astronomer Naomi McClure-Griffiths identified a new spiral arm of the Milky Way galaxy

Radiation

Radon (elemental)

Kinetic energy

Heavy elements in cosmic radiation

Beta particles are electrons

Nuclear shell

Astatine (elemental)

Nuclear fission - helped in the creation of nuclear weapons

Rhenium (elemental)

Seaborgium (elemental)

Polonium and radium (elemental)

Scotchgard

Structure of vitamin B12

Carbon Dioxide

Bioorthogonal chemistry - the concept of the bioorthogonal reaction has enabled the study of biomolecules such as glycans, proteins, and lipids.

Central heating

Square-bottomed paper bag

Correction fluid (white-out)

House solar heating

Wrinkle-free fiber

Windshield wipers

Car heater

Airplane mufflers

Underwater telescopes for warships

Written computer program

Written (programming) language

Chocolate chip cookies

Pizza saver

Mint chocolate chip ice cream

DNA structure

Sex chromosomes

Lactic acid cycle

Transporsable elements

Gap genes

Myers - Briggs Type Indicator

[did-you-fall-for-a-shooting-star]

Ya know, I generally despise biochem (biology and I are not friends). HOWEVER, one of my biggest role models in STEM is not just a biochemist, but the inventor of “bioorthogonal chemistry”.

Carolyn Bertozzi is SUCH a cool woman, she did her undergrad in chemistry at Harvard where she was ALSO in a band called Bored of Education with Tom Morello (the guitarist for Rage Against the Machine). She did her PhD at Berkeley working on synthesizing sugar polymer things (oligosaccharides). Did her post doc at the University of California where she did more indepth work with the sugar things. And she was the director of the Molecular Foundry at Berkeley for a few years!!

She currently does work with the sugary things and how they are related to things like cancer, infectious diseases, and arthritis.

There is SO much more she has done (like win a Nobel prize) and I highly recommend everyone take the time to read about her life and discoveries. She is amazing!

I am honored that my friends and I got the chance to meet her at the 2023 ACS conference in Indianapolis, and very much hope that I have more chances to hear her talk again in the future!

[dino-nugget7]

Stop Scrolling! I need people to know about someone!

Dr. Carolyn Bertozzi. Better. Fucking. Be. In. The. Science. History Books. She better be talked about in Biochem circles like Einstein is talked about among physicists.

Okay, so I've been out of the Chemistry loop for a hot minute, but the 2022 Chemistry Noble Prize winner Dr. Bertozzi, won for literally founding a brand new branch of Chemistry?!?!

It's called Bioorthogonal Chemistry, and it "refers to any chemical reaction that can occur inside of living systems without interfering with native biochemical processes." (Wikipedia page on the topic) This new field and technique allows researchers to chemically modify molecules in living organisms and not interrupt the processes of the cell.

But also, she's done a shit ton of major work on cancer and viral research, and founded multiple startups for things such as (noncomprehensive list): development of at home diagnostic tests for things like diabetes and HIV, and enhancing diagnostic tools for ovarian cancer.

She has over 600 publications and 54 awards and honors listed on her Wikpedia page. (I counted)

Oh, and she's also an incredibly talented musician who was offered multiple music scholarships in high school, was in a band with Tom Morello from Rage Against The Machine in undergrad, and has been out as a lesbian since the late 80s.

What a fucking woman. *swoons*

[cdmoservices]

[nordleuchten]

Carolyn R. Bertozzi, Morten Meldal and K. Barry Sharpless have just been awarded the 2022 Nobel Prize in Chemistry for theory works in click chemistry and biorthogonal chemistry … how cool is that!?

[exitwound]

bioorthogonal information storage in l-DNA with a high fidelity mirror-image Pfu DNA polymerase

[la-cosmonauta-extraterrestre]

Happy Women in STEM day (11 February)

Women of colour in STEM matter

Non-Western women in STEM matter

Immigrant women in STEM matter

Jewish women in STEM matter

Disabled and chronically ill women in STEM matter

Lesbian and bisexual women in STEM matter

Trans women in STEM matter

Women inventors matter

Women in STEM matter

(yes, many of these women fit into multiple categories)

Women pictured; I encourage people to learn more about them:

Katherine Johnson: mathematician and human calculator who worked for NASA

Mae Jemison: doctor, engineer and first black woman astronaut

Rebecca Cole: doctor and social reformer

Jane Cooke Wright: cancer researcher

Mary Golda Ross: First native American woman engineer; worked for Lockheed skunk works and in rocketry

Nergis Mavalvala: Astrophysicist who participated in first gravitational wave detection

Ginger Kerrick: NASA physicist and flight director

Monserrate Román: NASA microbiologist who contributed to developing the ISS

Mary Jackson: NASA computer and engineer

Tu Youyou: medical researcher who discovered some malaria treatments

Indira Nath: immunologist; leprosy researcher

Alexandra Elbakyan: programmer who created Sci-hub

Sarah Al Amiri: computer scientist, former environmental minister and current head of UAE space program

Sameera Moussa: nuclear physicist; studied applications in medicine and energy

Wang Zhenyi: astronomer, mathematician, poet, polymath

Olga Tsuberbiller: mathematician; wrote standardized geometry textbook for USSR high schools

P. K. Thressia: India’s first woman chief engineer

Noriko Arai: roboticist

Cecilia Payne: astronomer; wrote important thesis about stellar atmospheres

Chien-Shiung Wu: nuclear physicist

Irma Goldberg: chemist

Flossie Wong-Staal: virologist who studied HIV

Alexandra Olaya-Castro: astrophysicist and quantum biophysicist

Marie Skłodowska Curie: discovered radioactivity

Maria Goeppert Mayer: theoretical nuclear physicist

Sossina Haile: chemist, invented solid acid fuel cells

Sofia Kovalevskaya: mathematician and writer

Vera Rubin: astronomer who discovered dark matter

Emmy Noether: mathematician who contributed to theory of relativity

Marietta Blau: physicist; studied cosmic rays

Rosalind Franklin: chemist; charcoal specialist; radiographer who discovered structure of DNA as well as several viruses

Rosalyn Yalow: medical physicist

Esther Lederberg: microbiologist; bacterial geneticist

Mayana Zatz: geneticist and genetic counselor

Stephanie Horovitz: chemist; proved that isotopes exist

Joan Feynman: space weather researcher

Annie Jump Cannon: astronomer; invented classification system for stars

Henrietta Swan Leavitt: astronomer; discovered Cepheid variable stars

Chieko Asakawa: computer scientist; develops accessibility technology

Farida Bedwei: software engineer

Helen Taussig: pediatric cardiology pioneer

Susan La Flesche: doctor, public health advocate, native American advocate

Ada King, countess of Lovelace: mathematician, writer; wrote first computer program

Joan Ball: invented first computer dating service

Caroline Hershel: astronomer; discovered several comets

Sophia Jex-Blake: doctor; fought for women in medicine

Marina Logares Jiménez: mathematician and LGBTQ+ activist

Katie Mack: astrophysicist and cosmologist

Sally Ride: astrophysicist and first American woman in space

Carolyn Bertozzi: chemist; bioorthogonal chemistry pioneer

Renée Hložek: cosmologist

Josephine Baker: doctor; radical public health advocate

Margaret Todd: doctor; coined the term isotope

Nina Vedeneyeva: physicist

Lynn Conway: computer scientist, inventor, engineer and transgender activist

Audrey Tang: software developer and technological minister

Joan Roughgarden: ecologist, evolutionary biologist, wrote about LGBTQ+ in nature

Rebecca Oppenheimer: astronomer

Martine Rothblatt: engineer, attorney, entrepreneur, biotechnologist, inventor

Robyn McCutcheon: astronomer, historian, engineer, foreign service worker (first to transition while abroad in foreign service)

Carys Massarella: doctor and transgender advocate

Autumn Kent: mathematician

Megan Povey: food physicist

Grace Hopper: computer scientist who invented COBOL, one of the first programming languages

Mary Sherman Morgan: rocket scientist; invented hydyne (type of rocket fuel)

Radia Perlman: computer programmer and network engineer who developed some internet protocols

Yvonne Brill: rocket scientist; invented hydrazine resistojet propulsion system

Stephanie Kwolek: chemist who invented Kevlar

Gertrude Elion: invented several medicines

Peggy Whitson: biochemist and astronaut

Cynthia Breazeal: roboticist and human-robot interaction researcher

Patricia Bath: ophthalmologist

Hypatia: astronomer, mathematician, philosopher and teacher at the Library of Alexandria

Margaret Hamilton: computer scientist; wrote program for Saturn V

Emma Haruka Iwao: computer scientist; holds record for calculating digits of pi

Lisa Randall: theoretical physicist

Nancy Roman: astronomer; NASA’s first chief of astronomy

Claudie André-Deshays Haigneré: doctor, politician and first French woman in space

Eunice Newton Foote: biologist, inventor, feminist; first person to realize that climate change is happening

Frances Kelsey: pharmacologist; prevented FDA from approving thalidomide

Sophie Germain: mathematician

[jhavelikes]

Stimuli-responsive biomaterials show great promise for modeling disease dynamics ex vivo with spatiotemporal control over the cellular microenvironment. However, harvesting cells from such materials for downstream analysis without perturbing their state remains an outstanding challenge in 3/4-dimensional (3D/4D) culture and tissue engineering. In this manuscript, a fully enzymatic strategy for hydrogel degradation that affords spatiotemporal control over cell release while maintaining cytocompatibility is introduced. Exploiting engineered variants of the sortase transpeptidase evolved to recognize and selectively cleave distinct peptide sequences largely absent from the mammalian proteome, many limitations implicit to state-of-the-art methods to liberate cells from gels are sidestepped. It is demonstrated that evolved sortase exposure has minimal impact on the global transcriptome of primary mammalian cells and that proteolytic cleavage proceeds with high specificity; incorporation of substrate sequences within hydrogel crosslinkers permits rapid and selective cell recovery with high viability. In composite multimaterial hydrogels, it is shown that sequential degradation of hydrogel layers enables highly specific retrieval of single-cell suspensions for phenotypic analysis. It is expected that the high bioorthogonality and substrate selectivity of the evolved sortases will lead to their broad adoption as an enzymatic material dissociation cue and that their multiplexed use will enable newfound studies in 4D cell culture.

User‐Controlled 4D Biomaterial Degradation with Substrate‐Selective Sortase Transpeptidases for Single‐Cell Biology - Bretherton - 2023 - Advanced Materials - Wiley Online Library

[tumimmtxpapers]

Bioorthogonal-Activated In Situ Vaccine Mediated by a COF-Based Catalytic Platform for Potent Cancer Immunotherapy

Personalized tumor vaccines have become a promising modality for cancer immunotherapy. However, in situ personalized tumor vaccines generated from immunogenic cancer cell death (ICD) and adjuvants are mired by toxic side effects and unsatisfactory efficiency. Herein, by functionalizing the reticular structure to optimize the catalytic activity of the materials, a series of biocompatible covalent organic framework (COF)-based catalysts have been designed and screened for establishing a... http://dlvr.it/SjvVXc

[mellowwastelandstranger]

DBCO Click Chemistry

DBCO click chemistry is a process of applying surface functionalization to a compound in order to change its structure and properties. This process involves a variety of methods, including PEI-DBCO and Azide-alkyne click chemistry. DBCO is a highly effective chemical agent that can be used to form solids, liquids, and polymers.

PEI-DBCO

DBCO (dibenzocyclooctyne) PEG acid is a heterobifunctional reactive PEG derivative. Its fast kinetics make it suitable for Click Chemistry reactions without metal catalysts. The heterobifunctionality of DBCO PEG derivatives enables them to be used for spontaneous biomolecule labeling. They are also stable in aqueous buffers. Here, we report the use of this compound to label aCD44 antibodies on mesoporous silica nanoparticles (MSNPs).

For the labeling of aCD44, aCD44 peptide was conjugated onto the aCD44 antibody. This peptide was attached onto a PEI nanoparticle through copper-free click chemistry. In this process, the azide moiety is covalently linked to the PEI-MSNPs via NHS ester linker. The azide moiety gives the peptide a positive charge. The PEI nanoparticles were then characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM) and zeta potential measurements.

The aCD44-PEI-MSNPs displayed a high internalization rate, which may account for the increased cytotoxicity. ACD44-PEI-MSNPs were more cytotoxic than PEI (25 kDa) and PEI-SS-CLs. The aCD44-PEI-MSNPs also showed a higher efficiency in gene transfection, while the PEI-SS-CLs were less cytotoxic.

Azide-alkyne click chemistry

During the past decade, copper catalyzed azide-alkyne click chemistry has become an increasingly popular reaction in the science community. This reaction provides a method for linking different types of peptides, antibodies, and drugs. This method can also be used to develop biomolecules that can be targeted in a complex chemical environment.

Click chemistry has gained popularity because it is a versatile and modular reaction, which is useful for a variety of applications. Click reactions are also quick, reliable, and selective. They can be used to target selected cellular organelles, such as mitochondria. They are also an effective tool for studying the biological effects of Pt-based anticancer drugs.

Click chemistry is also used for drug discovery and development. ABP Biosciences has developed fluorescent dyes for use in click chemistry. They also have developed a biotin that is CLICKable.

Azide-alkyne click chemistry is classified into two main categories, the Cu(I)-catalyzed reaction and the strain-promoted azide-alkyne click (SPAAC) reaction. In SPAAC, the cycloalkyne precursor is oxidized by a reagent such as a gem-fluorine to release its ring strain, thereby increasing the reaction driving force. This enables the high fidelity ligation of alkynes with azides in a variety of chemical environments. The SPAAC reaction also allows for high efficiency ligation without the use of a catalyst.

Surface functionalization

DBCO click chemistry is a novel bio-conjugation strategy. This strategy provides a simple way to conjugate molecules such as enzymes and antibodies to surfaces of nanoparticles. These molecules can be used as signal probes for imaging and biosensing applications. Click chemistry also provides a flexible framework for controlling the conjugation process. It can also improve the analytical performance of nanosensors used in bio-analysis.

DBCO compounds react with azide functionalized compounds without the need of a Cu(I) catalyst. The resulting triazole linkage provides a stable connection between azide groups and azide ligands. This reaction was first identified in 1953 by Blomquist and Liu. Later, Wittig and Krebs confirmed the reaction in 1961.

Bioorthogonal click chemistry has been used to conjugate recognition elements to SMNPs. Using this strategy, researchers have developed QDs as multiple signal probes. These QDs are able to detect individual virus particles. They can also be used to detect circulating tumor cells.

Bioorthogonal click chemistries have made it possible to detect multiple biomarkers in a single sample. In addition, their combination with lateral flow devices can reduce the cost of nanosensors and shorten the analysis time.

oYo-Link DBCO

oYo-Link is a site-specific protein labeling solution that is activated by non-damaging black light. This product is designed to eliminate the shortcomings of conventional protein labeling techniques. Instead of requiring sample concentration and centrifugation, oYo-Link enables you to label just a single ug of antibody at a time, resulting in a high affinity and 100% sample recovery.

oYo-Link can be used in buffers, and is compatible with Tris, BSA, and BSA-Tris buffers. When illuminated by the non-damaging black light, the oYo-Link reagent forms a covalent bond with the antibody. This bond forms in just two hours, and is ready for storage. Once the reaction is complete, the antibody will be labeled with the desired label. It is also a copper-free click chemistry solution. It can be used with azide-tagged molecules, and it can be run in aqueous and organic solvents.

AlphaThera's oYo-Link Conjugation Technology can be used to label the Fc region of an antibody using click chemistry or enzymes. It can also be used to label an antibody with oligonucleotides or biotin.

[sounmashnews]

[ad_1] Sign up for CNN’s Wonder Theory science publication. Explore the universe with news on fascinating discoveries, scientific advancements and more. CNN  —  The Nobel Prize in chemistry was awarded Wednesday for work that led to “an ingenious tool for building molecules.” Carolyn R. Bertozzi, Morten Meldal and Ok. Barry Sharpless grew to become Nobel laureates for founding and advancing the fields of click on chemistry and bioorthogonal chemistry, which has “led to a revolution in how chemists think about linking molecules together,” the Royal Swedish Academy of Sciences mentioned. Click chemistry permits quick and simple reactions, the place “molecular building blocks snap together quickly,” the committee mentioned. The precept can convey real-world advantages within the growth of prescription drugs and drugs, together with extra focused most cancers therapies. Sharpless and Meldal pioneered the idea earlier than Bertozzi “took click chemistry to a new level,” the organizers mentioned, by growing click on reactions that work inside dwelling organisms (or bioorthogonal reactions). Click chemistry brings two molecules collectively “almost like a couple of pieces of Lego,” American Chemical Society President Angela Wilson instructed CNN on Wednesday. “Bertozzi was responsible for doing click chemistry within the human body, which is absolutely remarkable,” Wilson mentioned. “The three together have opened up new doors for us in what we can do, from pharmaceutical chemistry to materials chemistry,” and their work is already being utilized in manufacturing, she added. Bertozzi instructed reporters on the winners’ press convention over the cellphone that her developments are getting used “to discover new kinds of molecules we didn’t know existed,” and means scientists are “doing chemistry inside human patients to make the drugs go to the right place.” The Stanford University professor mentioned she was instructed of her win in the course of the evening on the American west coast. “I can hardly breathe,” she mentioned of her response. “I’m still not entirely positive it’s real.” Sharpless, a professor of chemistry at Scripps Research in La Jolla, California, in the meantime, grew to become the fifth particular person ever to win two Nobels, becoming a member of a brief record that features pioneering chemists Marie Curie and Frederick Sanger. Sharpless got here up with the idea for click on chemistry in 2001, the identical 12 months he received his first Nobel prize for chemistry. Working independently, Sharpless and Meldal, who is predicated on the University of Copenhagen in Denmark, got here up with what the Nobel committee described because the “brilliant reaction” synonymous with click on chemistry, which entails a catalyzed response between azides and alkynes. “If chemists want to link two different molecules they can now, relatively easily, introduce an azide in one molecule and an alkyne in the other. They then snap the molecules together with the help of some copper ions,” the Nobel committee mentioned in a doc explaining why that they had awarded the prize. “It’s used to modify materials, for instance if you want them to conduct electricity or collect light or modify surfaces to become antibacterial,” defined Johan Elf, a member of The Royal Swedish Academy of Sciences and a part of the committee that determined the respect. Bertozzi developed click on reactions that can be utilized inside dwelling organisms – as a result of they don’t contain copper ions that are poisonous. She centered on glycans – advanced carbohydrates which can be constructed from varied forms of sugar and infrequently sit on the floor of proteins and cells. They play an essential function

in lots of organic processes, reminiscent of when viruses infect cells or when the immune system is activated, the Nobel committee defined. Just in! First selfie from our 2022 #NobelPrize laureate in chemistry @CarolynBertozzi. Congratulations! pic.twitter.com/kuremcRIaN— The Nobel Prize (@NobelPrize) October 5, 2022 Her bioorthogonal reactions – which happen with out disturbing the traditional chemistry of the cell – are used globally to map how cells operate. Some researchers at the moment are investigating how these reactions can be utilized to diagnose and deal with most cancers. “Potentially, you can direct a radioactive marker to cancer cells and you can potentially deliver a toxic or radioactive compound to kill the cancer cell. It’s also used to direct enzymes to cancer cells that remove the carbohydrate layer so the cancer cell can be seen by the immune system,” Elf instructed CNN. The Nobels are being awarded all through the week; the prizes for drugs and physics had been introduced on Monday and Tuesday. Bertozzi is the one feminine scientist to have been awarded a science Nobel prize this 12 months, after an all-male line-up in 2021. The three winners will share prize cash value $915,000 (10 million Swedish krona). [ad_2] Source link

[emoxnews]

Three Scientists Win Nobel Prize in Chemistry for Putting Molecules Together to Design Drugs | world news

Three Scientists Win Nobel Prize in Chemistry for Putting Molecules Together to Design Drugs | world news

The Nobel Prize in Chemistry was awarded to three scientists who developed a method of “sticking molecules together” to create new drugs. “Click chemistry” or bioorthogonal reactions can be used to create new anticancer drugs and map DNA. It is the work of US scientists Carolyn R. Bertozzi and K. Barry Sharpless and Morten Meldal of Denmark. Mr. Sharpless of Scripps Research in California won the…

[cdmoservices]