#DNA methylation

[sproutystudies]

DNA Methylation

Hello! This week's educational post is on DNA methylation. Note that DNA methylation functions a bit differently in plants vs animals. This post will be more plant-centric since that is what I study.

DNA methylation refers to the addition of a methyl group (one carbon molecule) to the 5' position of a cytosine base in the DNA. This is a type of epigenetic mark, or a heritable genetic modification without a change in DNA sequence (Takuno & Gaut, 2012). It alters the expression of genes through transcriptional silencing, usually by preventing the binding of polymerase. However, the exact ways methylation regulates and is regulated can differ depending on context (Neiderhuth & Schmitz, 2016). There are three contexts in which DNA methylation can occur: CG, CHG, and CHH, where H represents A, T, or C (Neiderhuth & Schmitz, 2016).

Methylation in the CG context, known as CpG methylation, is the most stable of the three contexts. After DNA replication occurs, VIM1-5 recognizes hemi-methylated CG sites and recruits the methyltransferase MET1 to methylate the unmethylated strand (Neiderhuth & Schmitz, 2016). CpG methylation around the transcriptional start site causes transcriptional silencing, while CpG methylation in the gene body is associated with active, and even increased, transcription (Neiderhuth & Schmitz, 2016).

Methylation in the CHG context is the second most stable. It is maintained by the methyltransferase CMT3, which depends on the H3K9me2 histone modification (I'll go over histone modifications for my next educational post) in a positive feedback loop that maintains the locations of both these epigenetic marks (Neiderhuth & Schmitz, 2016). This feedback loop can be broken by the histone demethylase IBM1, causing transcription to become reactivated (Neiderhuth & Schmitz, 2016).

CHH methylation cannot be maintained after DNA replication due to its asymmetry: lack of a guanine on the methylated strand means there is no cytosine to methylate on the opposite strand (Neiderhuth & Schmitz, 2016). CHH methylation is always established de novo by the methyltransferase CMT2 or through the RNA-directed DNA methylation (RdDM) pathway (Neiderhuth & Schmitz, 2016).

De novo DNA methylation for all three contexts can be mediated by the RdDM pathway (Zhang et al, 2018). In this process, short-interfering RNAs (siRNAs) work together with scaffold RNAs and a variety of proteins to guide the methyltransferase DRM2 to target regions for methylation (Zhang et al, 2018).

Transcriptional regulation through DNA methylation is important for a variety of processes related to plant development and genome stability. Specifically, it has been shown to be involved in transposon silencing (Slotkin & Martienssen, 2007), fruit ripening (Xiao et al, 2020), genetic imprinting (Rodrigues et al, 2013), and aging (D’Amico-Willman et al, 2024).

(Neiderhuth & Schmitz, 2016)

Important Terms: DNA methylation, epigenetics, cytosine, transcription, methyltransferase

[turbulencegains]

Folic Acid and Cancer Risk: A Comprehensive Vegetarian Review of Current Evidence

“Whether you’re bench-pressing a barbell or briefing the A320, your folate game is mission-critical—get it right, and you soar; get it wrong, and you stall.” I’m Jay: vegetarian powerlifter by sunrise, cadet pilot by daylight. My diet—rajma-chawal, palak-paneer, moong-dal khichdi—fuels deadlifts and flight sims alike. But when it comes to folic acid and cancer, balancing natural folate from…

[biology-geology-beaches-india]

TSRNOSS, p 634.

[unopenablebox]

it's literally such a red flag if someone's into primatology and epigenetics

[xcodelifedna]

Unlocking Your Genetic Blueprint for Heart Health: What Your DNA Reveals

Explore how Gene Health DNA reports can identify your genetic predisposition to heart disease, stroke, and cholesterol issues. Discuss key genes like APOA2, PPARD, and HFE influencing heart health. Highlight how personalized nutrition and fitness plans (Gene Nutrition and Fitness DNA reports) can mitigate risks. Mention MTHFR and methylation’s role in cardiovascular health.

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GI MAP Test Topeka KS: Unlocking Gut Health with Epic Integrative Wellness

Understanding the GI MAP Test

In the ever-evolving world of functional medicine, gut health has emerged as a cornerstone of overall wellness. The GI MAP Test Topeka KS (Gastrointestinal Microbial Assay Plus) is a cutting-edge diagnostic tool that provides invaluable insights into the health of your digestive system. If you're in Topeka, KS, and seeking answers to persistent digestive issues, Epic Integrative Wellness offers comprehensive GI MAP testing to help uncover the root causes of gut imbalances and guide personalized treatment plans.

What is the GI MAP Test?

The GI MAP Test is a comprehensive stool analysis designed to evaluate the microbiome, detect pathogens, assess digestive function, and identify inflammation markers. This advanced test uses quantitative PCR (polymerase chain reaction) technology to detect microbial DNA, providing an accurate and detailed assessment of your gut health.

Unlike conventional stool tests, which often rely on culturing techniques, the GI MAP Test delivers more precise and comprehensive data, making it an invaluable tool for those struggling with chronic digestive issues, autoimmune conditions, and systemic inflammation.

Why is Gut Health So Important?

The digestive system plays a crucial role in maintaining overall health. A well-balanced gut microbiome supports immune function, aids in nutrient absorption, and contributes to mental well-being. However, factors such as poor diet, chronic stress, antibiotic overuse, and environmental toxins can disrupt gut balance, leading to a range of health issues, including:

Chronic bloating and gas

Diarrhea or constipation

Food intolerances

Acid reflux or GERD

Leaky gut syndrome

Autoimmune disorders

Skin conditions like eczema or acne

Mood disorders such as anxiety and depression

If you're experiencing any of these symptoms, the GI MAP Test at Epic Integrative Wellness in Topeka, KS, can help identify underlying issues and pave the way for a targeted treatment approach.

What Does the GI MAP Test Measure?

The GI MAP Test provides a comprehensive analysis of various gut health markers, including:

1. Pathogenic Bacteria, Parasites, and Viruses

Identifies harmful bacteria such as Clostridium difficile, Salmonella, and E. coli.

Detects parasites like Giardia and Cryptosporidium.

Screens for viral pathogens like Norovirus and Rotavirus.

2. Beneficial and Opportunistic Bacteria

Assesses levels of good bacteria (Lactobacillus, Bifidobacterium, Akkermansia muciniphila).

Identifies overgrowth of opportunistic bacteria that may disrupt gut balance.

3. Fungal and Yeast Overgrowth

Detects yeast and fungal infections such as Candida albicans.

Helps determine if an overgrowth is contributing to gut dysbiosis.

4. Inflammation and Immune Markers

Measures calprotectin, a marker of intestinal inflammation.

Assesses secretory IgA, which indicates immune function in the gut lining.

5. Digestive Function and Enzyme Levels

Evaluates pancreatic elastase, an enzyme essential for proper digestion.

Measures steatocrit to detect fat malabsorption issues.

6. Leaky Gut Markers

Assesses the presence of Zonulin, a protein that regulates gut permeability.

Helps determine if intestinal permeability is contributing to systemic inflammation.

Who Should Get a GI MAP Test?

The GI MAP Test is beneficial for individuals experiencing:

Chronic gastrointestinal symptoms (bloating, diarrhea, constipation, gas)

Suspected food intolerances or sensitivities

Autoimmune disorders such as Hashimoto’s thyroiditis or rheumatoid arthritis

Persistent skin issues like acne, psoriasis, or eczema

Mood disorders, including anxiety and depression

Chronic fatigue or fibromyalgia

Unexplained weight gain or difficulty losing weight

At Epic Integrative Wellness in Topeka, KS, we use the GI MAP Test to create tailored treatment plans that restore gut health and optimize overall well-being.

The Testing Process: What to Expect

Consultation – At Epic Integrative Wellness, we begin with an in-depth consultation to assess your symptoms, medical history, and lifestyle factors.

Stool Sample Collection – You’ll receive an at-home test kit with clear instructions on how to collect a stool sample.

Lab Analysis – Your sample is sent to a specialized lab for comprehensive analysis.

Results & Interpretation – Our team will review your results and provide a detailed breakdown of findings.

Personalized Treatment Plan – Based on your results, we’ll design a customized protocol that may include dietary changes, probiotics, supplements, and lifestyle modifications.

Benefits of GI MAP Testing at Epic Integrative Wellness

Precision & Accuracy – Unlike traditional stool tests, the GI MAP Test uses advanced DNA technology for highly accurate results.

Early Detection – Identifies gut imbalances before they lead to chronic health conditions.

Personalized Approach – Customized treatment plans based on individual gut profiles.

Comprehensive Care – Integrative strategies that go beyond symptom management to address root causes.

Expert Guidance – Our experienced practitioners provide ongoing support to help you achieve lasting gut health improvements.

Take Charge of Your Gut Health Today!

If you’re struggling with digestive issues, unexplained symptoms, or chronic health concerns, the GI MAP Test at Epic Integrative Wellness in Topeka, KS, can provide the answers you need. Understanding your gut microbiome is the first step toward reclaiming your health and vitality.

Contact Epic Integrative Wellness today to schedule your GI MAP Test and take the first step toward a healthier, happier you!

[thelovebudllc]

Research explores DNA methylation clock accuracy across tissues

A new research paper was published in Aging (Aging-US) on January 3, 2025, in Volume 17, Issue 1, titled “Characterization of DNA methylation clock algorithms applied to diverse tissue types.” Researchers Mark Richardson, Courtney Brandt, Niyati Jain, James L. Li, Kathryn Demanelis, Farzana Jasmine, Muhammad G. Kibriya, Lin Tong, and Brandon L. Pierce from the University of Chicago and University…

[blackbiobiotech]

Reliable MGMT Methylation and HPV PCR Kits for Oncology and Women’s Health

Molecular diagnostics play a crucial role in cancer research and infectious disease detection. MGMT promoter methylation testing helps determine treatment response in cancer patients, while HPV DNA testing is essential for identifying high-risk human papillomavirus infections. 3B BlackBio Biotech provides cutting-edge diagnostic kits, including the MGMT Methylation Detection Kit and HPV PCR Kit, designed for accurate and efficient genetic analysis.

MGMT Promoter Methylation and Its Role in Cancer

The MGMT (O6-methylguanine-DNA methyltransferase) gene is responsible for repairing DNA damage caused by alkylating agents. When the MGMT promoter is methylated, the gene becomes inactive, preventing DNA repair and making cells more sensitive to chemotherapy. This alteration is commonly seen in glioblastoma and other cancers, influencing treatment strategies.

Testing for MGMT methylation is critical in determining whether a patient will respond to alkylating chemotherapy drugs like temozolomide. Patients with methylated MGMT promoters typically show better treatment outcomes, making this biomarker valuable for personalized medicine. By identifying MGMT methylation status, oncologists can make informed decisions about the best therapeutic approach.

MGMT Methylation Detection Kit by 3B BlackBio Biotech

The MGMT Methylation Detection Kit is a highly precise tool designed to analyze MGMT promoter methylation using real-time PCR technology. This method ensures accurate and reliable results, helping researchers and clinicians determine methylation levels efficiently. The kit is optimized for high sensitivity, detecting even low levels of methylation, making it a trusted choice in oncology research and diagnostics.

With the increasing need for precision medicine, MGMT methylation PCR testing plays an essential role in guiding treatment decisions. The ability to accurately assess this biomarker helps in predicting patient response and improving overall survival rates in glioblastoma and other cancers.

The Importance of HPV DNA Testing in Women's Health

Human Papillomavirus (HPV) is the leading cause of cervical cancer, with HPV-16 and HPV-18 being the most high-risk strains. Early detection of these strains is crucial in preventing the progression of HPV infections into cancerous conditions. Traditional Pap smears are useful but may not always detect HPV infections at an early stage, making molecular testing a more effective approach.

HPV PCR testing provides highly sensitive detection of viral DNA, allowing for early diagnosis and better management of infections. Regular screening helps in identifying high-risk HPV cases, enabling timely intervention and reducing cervical cancer risks. As more healthcare providers adopt PCR-based HPV testing, the ability to diagnose infections with high accuracy is improving significantly.

HPV DNA Test Kit by 3B BlackBio Biotech

The TRUPCR® HPV 16/18 Genotype Kit is designed for the specific detection of high-risk HPV strains using real-time PCR. This advanced kit ensures rapid and accurate identification of HPV-16 and HPV-18, aiding in early diagnosis and effective patient management.

By providing a highly specific and sensitive method for HPV detection, this kit supports both clinical and research applications in women’s health. The real-time PCR approach allows for the precise quantification of viral load, offering valuable insights for risk assessment and treatment planning.

Conclusion

MGMT promoter methylation testing is a critical component in cancer diagnostics, helping predict chemotherapy response and guide treatment decisions. HPV DNA test kit plays an equally important role in women's health, detecting high-risk strains that can lead to cervical cancer. 3B BlackBio Biotech provides advanced molecular diagnostic solutions, ensuring accurate and reliable testing with their MGMT Methylation Detection Kit and HPV PCR Kit.

With the growing demand for precision diagnostics, these PCR-based kits serve as essential tools in research and clinical applications. For those looking for reliable genetic testing solutions, 3B BlackBio Biotech stands as a leader in molecular diagnostics.

[medicomunicare]

FOXes R 2 clever to go undetected: but when it comes to children, "targeted" shots are needed

Pediatric tumors are a field of oncology that has always had special attention that needs no explanation. Tumors such as neuroblastoma and medulloblastoma are among the most frequent tumors of the nervous system in children and basic research is making great strides in understanding the molecular defects affecting these types of cancer. For example, it has been understood that among the thousands…

[biology-geology-beaches-india]

The Research Manuscripts. Page 150.

[johniac]

SciTech Chronicles. . . . . . . . .Feb 13th, 2025

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