#Laboratory Sample Collection

[clinfinitesolution]

Clinical Research Institute for Advancing Healthcare

Introduction

Clinical research institutes play a vital role in advancing medical knowledge, developing new treatments, and improving patient care worldwide. These institutions conduct scientific studies to evaluate the safety and effectiveness of medical treatments, shaping the future of healthcare.

What is a Clinical Research Institute?

A clinical research institute is a specialized organization dedicated to conducting medical studies that evaluate new drugs, therapies, and interventions. These institutes collaborate with pharmaceutical companies, government agencies, and healthcare providers to bring innovative treatments from the laboratory to real-world applications.

The Importance of Clinical Research

Clinical research is the backbone of modern medicine, ensuring that new treatments are safe, effective, and beneficial for patients. It provides scientific evidence that helps healthcare professionals make informed decisions about patient care, leading to better health outcomes and the development of breakthrough medical solutions.

Key Areas of Focus in Clinical Research

Drug Development and Trials

One of the primary functions of clinical research institutes is to oversee the development and testing of new medications. These trials help determine the safety, dosage, and efficacy of new drugs before they reach the public.

Disease Prevention and Public Health Studies

Research institutes also focus on understanding disease patterns and developing strategies to prevent illnesses. Public health studies analyze factors that contribute to disease outbreaks and help create effective prevention programs.

Medical Device Innovation

Beyond pharmaceuticals, clinical research extends to evaluating medical devices and their impact on patient care. From surgical instruments to wearable health technology, research ensures these innovations are safe and beneficial.

The Phases of Clinical Trials

Clinical trials follow a structured process, moving through different phases to ensure treatment safety and effectiveness.

Phase I: Safety and Dosage Testing

The first phase of clinical trials focuses on determining the appropriate dosage and potential side effects of a new treatment. A small group of healthy volunteers is usually involved in this stage.

Phase II: Evaluating Effectiveness

Once safety is established, Phase II trials assess how well the treatment works for a specific condition. This phase involves a larger group of patients to measure its effectiveness and identify any side effects.

Phase III: Large-Scale Testing

At this stage, trials expand to larger populations to confirm effectiveness and monitor side effects. If successful, the treatment moves forward for regulatory approval.

Phase IV: Post-Market Surveillance

Even after approval, ongoing research ensures long-term safety and effectiveness in real-world use. This phase helps detect any rare or long-term side effects that may not have appeared in earlier trials.

The Role of Regulatory Bodies in Clinical Research

Government agencies such as the FDA (U.S. Food and Drug Administration) and EMA (European Medicines Agency) establish strict guidelines to protect patients and uphold ethical research practices. These organizations review trial results, approve new treatments, and monitor post-market safety to ensure compliance with global health standards.

Challenges in Clinical Research

Ethical Considerations

Ensuring patient safety, informed consent, and ethical research practices remain top priorities. Researchers must adhere to strict ethical guidelines to protect participants and maintain trust in the scientific community.

Recruitment and Retention of Participants

Finding and keeping participants engaged in long-term studies can be challenging. Researchers must create awareness, offer incentives, and ensure trial protocols are patient-friendly to maintain participation.

Funding and Resource Allocation

Clinical research requires significant investment, and securing funding is often a major hurdle. Institutes rely on government grants, private investments, and industry partnerships to sustain research efforts.

The Future of Clinical Research Institutes

With advancements in artificial intelligence, precision medicine, and global collaboration, the future of clinical research looks promising. AI-driven data analysis is streamlining research processes, while personalized medicine is leading to targeted treatments based on individual genetics. International partnerships are also accelerating the development of new therapies by sharing resources and expertise.

Conclusion

Clinical research institutes continue to shape the future of medicine, driving innovation and improving lives through groundbreaking discoveries. Their work not only enhances our understanding of diseases but also paves the way for more effective and safer treatments, ultimately transforming healthcare worldwide.

[conferkare]

Bhubaneswar laboratory pathology services- ConferKare

Experience the convenience of fast and accurate online pathology testing with our services available in Bhubaneswar, Hyderabad, and Cuttack. 

Skip the hassle of traditional lab visits and get your pathology tests done from the comfort of your home. Our state-of-the-art laboratories ensure precise and reliable results for all your health needs, from routine blood tests to comprehensive full body check-ups. 

With easy online booking and home sample collection, managing your health has never been easier. Trust us for timely reports and expert care. Book your online pathology test today and take a proactive step towards better health

Book Your Online Pathology Test Now in your area. For More call Us Today!! 9776414444 / 91 637 1966 229

[ccurehealth]

Know more about reproductive diagnostics tests and why it is important at CcureHealth.

[fading-event-608]

Recently the syndicate of chemists in Lebanon has issued a statement warning people to not go near the blast sites due to alleged use of depleted uranium by Israel. (link - you need to scroll till the statement in Arabic). The screenshot of their statement on twitter was shared here on Tumblr and I’ve seen multiple people expressing scepticism regarding the source. Some people linked an article (link) from anti-Hezbollah 'democratic' newspaper 'L’Orient Today' to ‘fact-check’ - because of course they can’t read Arabic and are discontent with a twitter link.

This is my short summary of the article: they confirm that Israel has used Depleted Uranium (DU) weapons, not only in Lebanon but also in Gaza in June of this year and between October and December of last year. They establish a history of the use of Depleted Uranium, and include examples of its use in Iran in 2003. Israel doesn't directly talk about their use of DU, but neither are they hiding it - because there is no law that forbids the use of these bombs by Israel, there is no treaty regulating the use of DU weapons. There were several resolutions calling for a moratorium on the use of DU weapons in the UN and EU Parliament, the latest of which was in 2022, but these have failed to stop their use (those who have used them also includes both Russia and Ukraine). The article ends with an ominous addition that the Israeli army has been found guilty multiple times of using white phosphorus, which IS prohibited against civilians or civilian property under international law. (You probably can already tell that their defense is that they do not use it against civilians)

There is another article that was published in early September this year - LINK - I highly encourage you to read this one yourself, as it is quite short, especially when considering the amount of information it contains. As this one is more easily accessible, I won’t summarize it - please take it in yourself. I will say, however, that this article’s author, one Dr. Busby, worked with colleagues to conduct several investigations into the use of uranium-based weapons in both Lebanon and Gaza. In 2006, Dr. Busby asked his colleague to collect multiple samples from a crater left by what was suspected to be Depleted Uranium weapons. Samples from an ambulance air filter were also taken. Dr. Busby and company found not only the  presence of depleted uranium but also of Enriched Uranium. Here’s the paper: link.

Enriched Uranium. In 2006.

By 2024, all of the laboratories that Dr. Busby had used to Conduct the investigation have closed their doors either to him or in general. Busby’s letters to the UN, as well as papers detailing evidence of the use of enriched and depleted uranium are either dismissed or ignored, rendering it unlikely that there will ever be the “official” source for these claims that certain people now see fit to demand. And even if the UN did accept those letters and did push for ban of those weapons - would Israel comply? Genocide is ‘illegal’ under international law, and Israel still faces the case in ICJ, but what will that ICJ do if they rule that Israel is guilty? What would UN do if they accept evidence of Israel using uranium-based weapons? Scold them and write a fine?

The aspect of the deployment of nuclear weapons considered the most horrific is - and has always been - the fallout. The idea that all nuclear weapons would leave evidence - again, fallout - behind was born into the cultural consciousness through various cold war era PSAs, as well as other media inspired by these horrors, potential and otherwise. The weapons Israel is using here do not create fallout, however. But do not mistake them as harmless - they are still highly carcinogenic. They cause birth defects, as well as various other illnesses - mysterious illnesses, or at least mysterious until doctors attempting to treat them register that their patients have been exposed to enriched uranium, after which point the mystery goes away. 

In a sense, the horrors advertised by cold war PSAs and films like Doctor Strangelove, the promise of some explosive end brought about by some fool in the US pushing the wrong button - these serve to draw a veil over the continued use of nuclear weapons that have been ongoing since this technology was first harnessed for violence. This is a severe danger to the people of Gaza, and we can’t ignore it simply because we have developed in our minds too much faith in the loosest understandings of nuclear warfare.

I think many of you are familiar with a boiling frog story. The story goes that if you put a frog in a pot of boiling water, it will try to climb out. But if you put it in warm water and very slowly heat it, it will be so accustomed to the temperature it will eventually be boiled alive. It’s not very authentic, of course - in reality the frog will try to jump out as soon as it deems the water temperature uncomfortable. Just like you would try to get out of the bathtub as soon as it gets too hot for you or try to warm yourself up when you spend too much time outside in winter. 

But some of it still rings true. At what point will the UN, or ICJ, or some other white savior wannabe decide that Israel has done too much? What is that ‘too-much’ point that makes them try to protest, and what would that protest be?

As in case with Tumblr, it seems that the boiling point, in fact, has already passed and people grew accustomed to deaths of Palestinians. There are thousands of posts about the situation in Gaza, and the whole Palestine, Lebanon, Yemen, Syria… They get a lot of attention by both zionists and Palestine supporters. There are also hundreds of Gazans that came to Tumblr in hopes to escape the genocide by asking people to cover evacuation and survival costs. Do they get the same attention? Barely. Arguably zionists are more invested in interacting with those posts - they mass report them and harass Palestinians. And even if the fundraiser post gets a lot of attention, it does not necessarily translate into a lot of donations - people just assume that someone else will donate instead of them.

You can’t stop Israel all by yourself. You can’t convince the UN or try to progress the ICJ case by yourself. You can, however, do small acts that will contribute to Palestinian resistance. Go protest, go boycott, and please, please, please, go donate to Palestinian fundraisers.

Falastin’s family are under constant threat in Gaza. She’s been fundraising to save them since late June, and yet they’ve only recently gotten to just over 5% of their total goal - a little short of $10,000 USD. They’re still in Gaza, and still in need of funds for survival. The longer they are trapped there, the more they need - not just for food and water, but also for medicine, shelter, and clothes. Each time they’re displaced, due to inadequate time to pack, they lose more supplies, and their needs increase. Give what you can so that they can survive this, and please share their fundraiser as much as you’re able regardless of whether you can donate, just in case someone you know might be able to help. Not just here on Tumblr, on other social media, talk to your friends, coworkers, family, in group chats and in discord servers.

Please keep in mind conversion rates before donating:

10$ = 103 SEK

25$ = 260 SEK

50$ = 519 SEK

100$ = 1,038 SEK

[market-insider]

Specimen Containers Market Emerging Trends: A Comprehensive Study

The global specimen containers market size is anticipated to reach USD 3.05 billion by 2030. The increasing prevalence of chronic disorders, technological advancements, and the rising geriatric population drive the market. 

Gain deeper insights on the market and receive your free copy with TOC now @: Specimen Containers Market Report

The COVID-19 pandemic in 2020 has positively affected market growth. The rise in swab specimen collection by laboratories has resulted in a significant increase in the demand for patient at-home sample collection services. In addition, collecting samples such as urine, sputum, and blood from patients is a common way to look for and diagnose illnesses such as coronavirus in the body. Due to this tendency, the market for specimen containers is growing in terms of revenue and sales volume. However, restrictions on environmental matters relating to the materials used and issues with paper and plastics in landfills are preventing industrial growth. 

The players focus on adopting various growth strategies, such as partnerships, mergers and acquisitions, and expansion of product portfolio. For instance, in December 2021, PPD, Inc., a U.S.-based global provider of clinical research services to the biotech and pharma industry, was bought by Thermo Fisher Scientific Inc. as part of the biopharma services and laboratory products segment for USD 15.99 billion in net cash consideration and USD 43 million in exchanged equity awards. The inclusion of PPD's clinical research services improves the company’s ability to serve biotech and pharmaceutical clients by allowing them to quicken innovation and boost output during the drug development process. PPD generated 4.68 billion USD in revenue in 2020, which is expected to increase the growth of the market. 

[reasonsforhope]

"A team of researchers at Washington University in St. Louis has developed a real-time air monitor that can detect any of the SARS-CoV-2 virus variants that are present in a room in about 5 minutes.

The proof-of-concept device was created by researchers from the McKelvey School of Engineering and the School of Medicine at Washington University...

The results are contained in a July 10 publication in Nature Communications that provides details about how the technology works.

The device holds promise as a breakthrough that - when commercially available - could be used in hospitals and health care facilities, schools, congregate living quarters, and other public places to help detect not only the SARS-CoV-2 virus, but other respiratory virus aerosol such as influenza and respiratory syncytial virus (RSV) as well.

“There is nothing at the moment that tells us how safe a room is,” Cirrito said, in the university’s news release. “If you are in a room with 100 people, you don’t want to find out five days later whether you could be sick or not. The idea with this device is that you can know essentially in real time, or every 5 minutes, if there is a live virus in the air.”

How It Works

The team combined expertise in biosensing with knowhow in designing instruments that measure the toxicity of air. The resulting device is an air sampler that operates based on what’s called “wet cyclone technology.” Air is sucked into the sampler at very high speeds and is then mixed centrifugally with a fluid containing a nanobody that recognizes the spike protein from the SARS-CoV-2 virus. That fluid, which lines the walls of the sampler, creates a surface vortex that traps the virus aerosols. The wet cyclone sampler has a pump that collects the fluid and sends it to the biosensor for detection of the virus using electrochemistry.

The success of the instrument is linked to the extremely high velocity it generates - the monitor has a flow rate of about 1,000 liters per minute - allowing it to sample a much larger volume of air over a 5-minute collection period than what is possible with currently available commercial samplers. It’s also compact - about one foot wide and 10 inches tall - and lights up when a virus is detected, alerting users to increase airflow or circulation in the room.

Testing the Monitor

To test the monitor, the team placed it in the apartments of two Covid-positive patients. The real-time air samples from the bedrooms were then compared with air samples collected from a virus-free control room. The device detected the RNA of the virus in the air samples from the bedrooms but did not detect any in the control air samples.

In laboratory experiments that aerosolized SARS-CoV-2 into a room-sized chamber, the wet cyclone and biosensor were able to detect varying levels of airborne virus concentrations after only a few minutes of sampling, according to the study.

“We are starting with SARS-CoV-2, but there are plans to also measure influenza, RSV, rhinovirus and other top pathogens that routinely infect people,” Cirrito said. “In a hospital setting, the monitor could be used to measure for staph or strep, which cause all kinds of complications for patients. This could really have a major impact on people’s health.”

The Washington University team is now working to commercialize the air quality monitor."

-via Forbes, July 11, 2023

-

Holy shit. I know it's still early in the technology and more testing will inevitably be needed but holy shit.

Literally, if it bears out, this could revolutionize medicine. And maybe let immunocompromised people fucking go places again

Also, for those who don't know, Nature Communications is a very prestigious scientific journal that focuses on Pretty Big Deal research. Their review process is incredibly rigorous. This is an absolutely HUGE credibility boost to this research and prototype

[rabotimagines]

Request for G1 Perceptor smut for @legendarycherryblossomlove

"Interest" GN BOT Reader x Perceptor

Summary: You tell your conjunx to keep info dumping while you keep your servos busy.

Genre/Theme: Smut scenario 🔞

Warnings: Semi public sex, Handjobs, MDNI

Pronouns: You, Your, Yours

Notes: Reader harasses their conjunx (Perceptor) just a bit

"Ah- it's good to see you this time of day." Perceptor smiled when you made your way into the group laboratory.

You come over to where he was working and leaned over his pauldron to stare at his petri dishes. Your frame gently pressing against his back, and your arms wrapping around him. Perceptor lets himself lean back against you, very much enjoying the soft touch of your affections. "I'm seeing how these samples react to our different solvents. So we can see what our approach towards cleaning different obstructions should be inclined towards."

Your servos had begun to idly trace the dips of his armor.

Your digits left soft warmth as they trailed along the lines that made up his boxy frame. "Of course, we can naturally use the information in case someone gets a bit of some natural material stuck in their struts. Or any of the other small areas that may be difficult to brush out the natural way. Like between the plating seams of your armor."

Your servos found his waist and moved to trace shapes on Perceptor's hips. "So if we simply figure out what solvents work the best, I'd like to craft a sort of spray nozzle that can cycle through the different solvents. For whenever one of us mechs gets unlucky and will need to be thoroughly clean- mph!" Perceptor abruptly jolts at the quick zap of light arousal, nearly biting down on his own glossia.

Perceptor hadn't realized your servos had trailed towards his modesty panel till you'd rubbed firmly against it. "Oh- What-?" You just tell Perceptor to keep talking. Your servos are now dragging along the dips and curves of his modesty panel.

"Oh... um, well yes-" Perceptor resets his vocalizor, his attention now drawn to how your servos and digits would trace the make up of his covered array. "So- to um, make a proper prototype, I need to test all the samples against all the solvents. And... as I stated, see which ones work the best against the other-" Perceptor's voice clips when you rub firmly against the flat of his modesty panel. Heat already circling boldly in his own array under the rather teasing act.

You were in the collective laboratory! Primus-! Anyone could rightfully just walk in on you two! Wheeljack could walk in behind the both of you, and you'd have no warning for it either!

Your frame presses flat against his back, and Perceptor's posture straightens out even more than it had been. Perceptor wordlessly watches your digits hook against the flat of his Modesty panel- heat swimming and pooling against his pelvis the longer you continued to tantalizingly grope at his covered array.

You ask him to open up, and Perceptor has to swallow the extra oral lubricant accumulating in his mouth.

.... Wheeljack wouldn't be too mad if he happened to stumble upon you two.

(At least Peceptor hoped he wouldn't-)

Perceptor's modesty panel pulls back, and his spike slowly pressurizes while you rub circles around his hips. You tell him to keep talking again when you cup his spike. Perceptor gasps and rushes to find his thoughts once more. "And it's quite fascinating that most the solvents don't have Ha-!" Your servo languidly begun stroking his spike. Perceptor's frame touches the front of the table edge, and he moves to grip it for stability.

"Most- most the solvents don't have a visible- a valid visible affect on the samples- ah, but when they sit soaked in them... they usually all seem to practically fall off the sample armor-" You grind your still closed modesty panel against Perceptor's aft, and he gasps.

Then you stopped moving when Perceptor had taken a klick to focus on venting.

Keep talking, you tell him once more.

Perceptor huffs before taking a moment to find his thoughts. His glossia sits heavy in his mouth. The last point he'd made of the experiment was much further from his processor than a few nano-klicks of silence.

But he did eventually find it again. "And- only one of the solvents-" Your servo speeds up, causing his pre lubricant to slick the inside your servo and begins to make an audible sound every time you'd pump your fist. "Hmph! Only one- one of the solvents has actually shown to- oh dear-!" Perceptors thighs pitch inward, and he leans against the table further.

You begin to slow when Perceptor stops and Perceptor rapidly moves his mouth to rectify that "One- One of the solvents has actually shown to break down the-" Perceptor grunts and moans when your other servo grabs onto the indent of his front chassis glass. And you physically used it as a hold to pull him back against your own frame. While you rocked your hips forward again against him.

"Break- break down all of the samples I have available on- oh Primus! On the sample armor!" You hum casually against him as if you were actually finding his findings intriguing and not- Perceptor pants and sighs leaning back against your also heated frame. You just rock against him harsh enough that he's practically forced to buck into your servo. Your servo dragging his own spike oh so hotly- Perceptor's own pleasure begins to bump up a level higher every time you pump his spike.

And you ask him how he's going to make the spray nozzle.

Perceptor can barely recall his plans for the spray nozzle, but he attempts to start explaining regardless, "I'm going to- Gha!" Your servo picks up, and any coherent thought relating to his findings gets pushed right out of Perceptor's processor. "I'm going to-!"  His hips buck against your servo, making more of his pre lubricant coat your servo. Your servo hooked on his chassis glass squeezes tighter and you use your hold to firmly tip his frame back against your own even further. You hold him against you when Perceptor started overloading onto the table.

Your designation ends up tumbling out of his mouth with a gasp. His overload hits him, and he's moaning against you and gasping for vents while you just continue to jerk his spike through his own high. "Oh- dear- oh Primus!" Perceptor mutters and arches against your frame and hold. His pedes twist against their placement on the ground, and his digit pads rank over the tables edge. His overload coursed through his frame with a pressing need, and Perceptor was unable to deny it the heady satisfaction.

Perceptor relaxes when his spike stops throbbing and charges stop shooting through his plating. "Primus." Perceptor pants and readjusts his grip on the tables edge to stand correctly once more. You hum and thank him for keeping up with your interest.

Perceptor can't help chuckling  "Yes well- now the price of your interest is you're going to have to get me a new sample of beach sand." Perceptor states before picking up the petri dish with the now ruined sample of sand to show you.

Ruined by his transfluid.

You sigh at the sight and say you'll go. And untangle yourself from Perceptor very obviously not excited by the task.

Perceptor supposed he could indulge his perverted conjunx just a touch more...

"I'll reward you when you get back if you do it quick enough." You perk up at Perceptor's words and make a dash for the door, saying you'll be back before he knows it.

Perceptor smiles, watching you rush for the door- only to jump when you barrel directly into Wheeljack, who was about to enter. Promptly sending the two of you tumbling down in the hallway. The laboratory door automatically shuts behind you both.

Oh dear- Perceptor moved to go help only to remember what you'd just done. His optics brighten, and instead, Perceptor subspaces his ruined sample and grabs a lab cloth to wipe the mess left on the table. He then makes sure he's modest before he goes to help you off of Wheeljack.

What was he going to do with you?

[clinfinitesolution]

Laboratory Sample Collection: A Comprehensive Guide

Introduction

Laboratory sample collection is a critical step in the diagnostic process, ensuring accurate and reliable results for patient care and research. Proper collection methods can significantly impact the quality of outcomes, making it essential to understand the nuances of this procedure. This guide delves into the key aspects of laboratory sample collection, covering types of samples, processes, challenges, and the role of technology.

Types of Laboratory Samples

Different types of samples are collected depending on the diagnostic needs, each requiring unique handling and techniques:

Blood samples: These include venous, arterial, and capillary blood, commonly used for biochemical, hematological, and serological testing.

Urine and stool samples: Non-invasive and essential for diagnosing infections, metabolic disorders, and gastrointestinal conditions.

Tissue biopsies: Collected for histopathological examination to detect abnormalities like cancer.

Swabs: Nasal, throat, or wound swabs are critical for detecting infections and pathogens.

Specialized samples: Cerebrospinal fluid, sputum, or synovial fluid may be collected for specific tests requiring advanced handling.

Key Steps in the Sample Collection Process

Proper sample collection involves a series of standardized steps to ensure quality and accuracy:

Preparing the patient and obtaining consent: Explain the procedure, address patient concerns, and obtain informed consent.

Selecting appropriate tools and containers: Use sterile and appropriate collection materials tailored to the sample type.

Following hygiene and safety protocols: Maintain aseptic techniques to prevent contamination and ensure the collector’s safety.

Labeling and documentation: Accurately label samples with patient information and record relevant details for traceability.

Best Practices for Sample Handling and Transportation

Proper handling and transportation of samples are vital to preserve their integrity and prevent contamination:

Use sterile containers to minimize contamination risks.

Maintain appropriate temperature controls, such as refrigeration or freezing, based on sample requirements.

Transport samples promptly to avoid degradation.

Ensure chain of custody documentation to maintain sample integrity and accountability.

Common Challenges in Laboratory Sample Collection

Despite advancements in techniques, challenges in sample collection can impact the quality of results:

Insufficient sample volume: Collecting inadequate amounts may require repeat procedures, causing patient discomfort.

Improper labeling or misidentification: Errors in labeling can lead to diagnostic inaccuracies.

Contamination risks: Poor aseptic techniques can compromise sample quality.

Handling errors during transport: Improper conditions may degrade the sample or make it unusable.

The Role of Technology in Sample Collection

Technology plays an increasingly important role in improving the efficiency and accuracy of laboratory sample collection:

Automated collection systems: Streamline processes and reduce human error.

Digital labeling and barcoding: Enhance traceability and minimize misidentification.

Integration of electronic health records (EHRs): Centralize patient data for seamless management.

Remote sample collection innovations: Enable home-based testing through advanced kits and devices.

Ensuring Patient Comfort and Safety During Collection

Prioritizing patient comfort and safety is essential for successful and stress-free sample collection:

Explaining procedures to patients: Helps alleviate anxiety and builds trust.

Minimizing discomfort during invasive collections: Use skilled techniques and appropriate tools to reduce pain.

Adhering to infection control practices: Protect both patients and healthcare workers from potential risks.

Regulations and Ethical Considerations in Sample Collection

Adhering to legal and ethical standards is crucial in laboratory sample collection to protect patient rights and data integrity:

Informed consent and confidentiality: Ensure patients understand and agree to the collection process.

Compliance with guidelines: Follow local and international standards for sample collection and handling.

Disposal of biological waste: Safely dispose of materials to prevent environmental and health hazards.

Conclusion 

Laboratory sample collection continues to evolve, with new technologies and techniques paving the way for enhanced diagnostics. Continuous training for healthcare professionals remains vital to uphold best practices and patient safety. Emerging trends like point-of-care testing and AI-powered diagnostics promise to revolutionize the field, making sample collection more efficient and accessible. By maintaining high standards and embracing innovation, the future of laboratory diagnostics looks promising.

Get more information here .....

https://clinfinite.com/LaboratorySampleCollection55.php

[todropscience]

MICROPLASTICS FOUND IN THE EXHALED BREATH OF DOLPHINS

Microplastic is a ubiquitous environmental contaminant, widespread across terrestrial and marine environments. In the environment, microparticles have been documented in a variety of fauna. Human epidemiological studies have found relationships between inhaled microplastics and oxidative stress, as well as inflammation.

Recent research has uncovered something quite striking: bottlenose dolphins in Florida and Louisiana are exhaling microplastic fibers. While plastic pollution has been well-documented in marine environments, this new study adds inhalation to the growing list of exposure routes for these animals. Bottlenose dolphins were found to be exhaling microplastics similar in chemical composition to those found in human lungs. Their exposure to microplastics might reveal broader environmental risks, especially for cetaceans health.

Breath samples were collected from wild bottlenose dolphins during health assessments conducted by scientists in collaboration with several organizations, including the Brookfield Zoo in Chicago and the Oceanogràfic Foundation. The researchers used a Petri dish or a custom spirometer to capture the dolphins' exhaled breath and, in the laboratory, analyzed the samples under a microscope for small plastic particles characterized by their smooth surface, bright colors, or fibrous shapes.

- A microplastic from a bottlenose dolphin's breath, nearly 14 times smaller than a human hair. Photo by Dziobak/College of Charleston.

The study found microplastics in dolphins from both urban and rural estuaries, demonstrating that even less-populated areas are not immune to plastic contamination. The key takeaway is that plastic pollution is a widespread issue, with effects that may be more far-reaching than initially thought. While the impacts on dolphins' lung health remain unknown, this research underscores the need to investigate how microplastic inhalation could affect marine life and humans alike. Reducing reliance on plastic is a crucial step toward addressing this growing problem.

Main photograph: Todd Speakman/National Marine Mammal Foundation.

Reference (Open access):  Dziobak et al. 2024. First evidence of microplastic inhalation among free-ranging small cetaceans. PLoS ONE

[conferkare]

Pathology Tests Online & Home Blood Collection- conferkare - India

Experience the convenience of fast and accurate online pathology testing with our services available in Bhubaneswar, Hyderabad, and Cuttack. 

Skip the hassle of traditional lab visits and get your pathology tests done from the comfort of your home. Our state-of-the-art laboratories ensure precise and reliable results for all your health needs, from routine blood tests to comprehensive full body check-ups. 

With easy online booking and home sample collection, managing your health has never been easier. Trust us for timely reports and expert care. Book your online pathology test today and take a proactive step towards better health Book Your Online Pathology Test Now in your area. For More call Us Today!! 9776414444 / 91 637 1966 229

[obsessivevoidkitten]

Love Stings

Yandere Male Aqrabuamelu (scorpion man) x Chubby Female Reader (CW: Violent Noncon, oviposition, paralytic venom, reader gets stung, stalking, stalking, scratching, biting, claiming bites, crying, mild scent kink, blood, mild blood kink, Stockholm syndrome, kidnapping, escape attempt, general yandere behavior) Word Count: 4.7k (This was commissioned at the end of September. It is hard for me to write afab reader. Don't worry though, I do not accept payment until I am done or almost done. The customer wished to remain anon. I hope everyone likes it.)

To most people, the wastelands of Treregar were just that, wastelands. Harsh sunlight, miles of seemingly endless sand, with water sources few and far between. Nothing but the hardiest flora and fauna could survive there for any significant length of time. Let alone thrive indefinitely.

But you were not most people. You were a scientist. And to scientists, the wastes were a gold mine of research potential. Some of the strangest creatures and plants called this place home. And with the country now open to outsiders, researchers wanted to make a breakthrough there. You were in search of biological samples that could have medicinal properties.

But the wilder parts of this country were very dangerous. Especially the desert wastes. There were even reports of explorers and scientists going missing and of large chitinous beasts prowling the sands. But you were not going to be deterred by a few tall tales. The others had probably fallen prey to the harsh environment if anything.

Still... it didn't hurt to have a couple bodyguards. You had arranged an escort of a couple fairly priced and reliable mercenaries, two large men by the names of Mr. Whitley and Mr. Matthews, to accompany you while you procured samples for study.

You also came with fully stocked packs and kept to a region near a small river. You didn't believe in letting stories run your life, but there was no need to take unnecessary risks either.

The first thing you did on your arrival, after setting up a base camp with your escorts, was starting your task by taking samples of the river water and the muck at the bottom. There was a good possibility of finding new microbes that could be of interest.

The water was crystal clear, perhaps the purest you had ever seen, and there were no signs of anything dangerous. The biggest danger was the heat. Despite the water submerging your legs and wetting your arms whenever you collected a sample the heat was still stifling.

But you had known what you were signing up for when you started all this and it would only get harder as you started collecting samples outside of the water.

When you collected plant and insect specimens from the fertile banks of the river you had to keep wiping the sweat from your brow. You kept the samples preserved in a specially cooled container that would allow you to study them at length later when you were back home.

Once you had all that you had gathered that day packed away, you ate alone in your tent, Mr. Whitley and Mr. Matthews were not exactly the best company, but that was okay. It wasn’t their companionship that they were being paid for.

When you had finished your meal, if you could even call one unit of mystery field rations a meal, you decided that it would be a good idea to go to bed early. Tomorrow’s adventure would be deeper into the wastes and you would need all the rest you could manage. It was going to be a bit rough on you. You were used to working in laboratory conditions not out in the field. But you knew what you were getting yourself into.

Whitley and Matthews guarded the camp in shifts, but even under their protection you couldn’t shake the feeling like you were being watched as you settled into an uneasy sleep.

On day two of the expedition, after eating another bland ration for breakfast and refilling your water supplies, you found something before you even set out. Odd tracks outside your camp, a good bit away but they were still close enough to creep you out a bit. Whatever had made them was not small.

But you had protection from any threats and you were handy with the knife you kept in your belt at all times. And whatever had made the tracks was gone now. You put it out of your mind and pushed onward.

As the cooler morning hours passed, you were really suffering as you had to start your trek into the desert proper. The organisms suited to such a brutal environment were what you were really interested in. Far more so than what lived in the relatively cool waters.

The sun beat down upon you with all the heat it could manage but still you trudged on. Miles and miles of sand. Stopping only to diligently take new samples and record field notes any time you encountered something new and promising.

You and your guards were overheated, exhausted, and aching. But it would surely be worth it for the scientific knowledge gained by your collective suffering. You all had finally started the long march back to camp when you could have sworn you felt like you were being watched. Whitley must have noticed something off as well as he stopped your trek home to look around. But the worry was discarded when in the distance a giant wall of sand materialized as if from nowhere, It rushed towards you like a great red tidal wave.

A sudden sandstorm.

It was upon you and your escorts in a thrice, you could see nothing at all in front of you as looking up for even a moment would invite the angry sands to scratch at your delicate eyes. From all your studies on the region in your preparation for this trip you had found sandstorms to be very rare this time of year. Your luck was astoundingly terrible.

All you could do in this situation was stay low to the ground, wait it out, and hope that you and your body guards didn’t get blown away. You also prayed that they both had the good sense to not try to walk in this mess. That would only welcome disaster. Trying to stumble about in this weather was a guaranteed method to find yourself lost and stranded in the desert.

On and on the storm raged, sand scraping and biting into any exposed flesh, until finally the whirling winds gradually declined before subsiding altogether. Leaving no trace of what had just transpired except scratches on your arms and legs and the ebbing adrenaline leaving your system rendering you shaky on your feet.

You were sore and stiff from laying in the same position so long. You took a good look around you only to discover that disaster had indeed been welcomed. Neither Whitley or Matthews were anywhere in your sight. Idiots. They were large men, not easily slipped up by the storm and rolled down a hill as you were. The weather couldn’t have pushed them very far. They clearly decided to keep trying to travel despite the obvious foolishness.

Well… they were not exactly getting paid for their brain power. But they were paid to keep you safe. To guard you. And they couldn’t exactly do that if they were determined to be idiots who wandered away from you!

When you made your report they were certainly getting docked for this.

Assuming that you all met back up. You probably would. Eventually. If they lacked a compass like you did then they could just head east until they hit the river and then travel up until they hit the camp. If they didn’t have one they would have to wait a little while and see what direction the sun headed in.

The direction the sun sets actually changes a bit throughout the year, but the autumn equinox had only just transpired so it would certainly set at the most west that it was possible for it to set.

You hoped those two knew that. Surely they did.

Right then you had to focus on yourself though. Without those two you were far more vulnerable. You were not really worried about the wildlife or anything such as that, but if you became injured or something all alone then you would be screwed. Something as simple as a sprained ankle could mean your death out in this blistering environment if you were left exposed to the elements long enough.

You shouldn’t have ventured so far from camp. Your body was aching with exhaustion. You were a bit on the chubby side and your body was not used to being pushed so hard. You perked up when you saw a large figure of a man standing in the distance walking in the same general direction as you were.

“WHITLEY! HEY, OVER HERE!!”

He evidently heard you as he turned around to face your direction and started walking towards you. As you got closer you could tell that other than a few abrasions from the storm he did not seem to be in bad shape.

“I am glad I found you”

You wanted to tell him he would not have had to worry about finding one another if he had just stayed put but you thought better of it and held your tongue.

“I think something may be watching us, I don’t know. I just have an uneasiness and feel like the air has shifted with a barely noticeable scent. I lost my sword in the storm.”

“Then… we better hurry back to camp… Maybe Matthews will be there…”

“Right.”

You noticed he had undone his dagger from his belt and was holding it close, ready to lash out if something attacked the two of you. He may not have had his longer blade but you knew he was still deadly with that one. He would surely protect yo-

The sand in front of you shifted, and from it sprung a beast of colossal size. Hard black chitinous plating gleamed in the sunlight, covering a monstrous form from top to bottom. It was a hulking beast with a human shaped torso on top of the body of a massive scorpion.

Whitley, to his credit, didn’t hesitate. He dodged the tail that struck at him with terrifying speed. He jumped up high and slammed his combat knife into the chitin covered chest of the beast. His blade was deflected as if by plate armor. The tail struck again at an angle Whitley couldn’t dodge in time and his arm was impaled by a wickedly sharp stinger. He twitched in pain for a moment then went limp before being pushed aside by the clawed arm of the scorpion half of the great beast before you.

Then the thing turned its gaze to you. Unblinking, glossy, entirely black eyes stared you down. If eyes were the windows into one’s soul then those eyes let you look into the soul of a demon.

Hopeless though it may be with the speed of the monstrosity you did the only thing your fear stricken brain could think of. You ran.

You ran and you didn’t look back. Cruel though it may have been, you found yourself thinking that maybe it would let you go since it had already taken down Mr. Whitley. Many predatory beasts did not take down more than what they needed.

But the scorpion hybrid wasn’t after food. Humans were not in his diet. No, he was after something much better than a meal. He was after a mate. A nice wet cunt to stuff full of his eggs.

When Kurnugi first caught your scent in the wind a couple days ago he was instantly intrigued, he had never smelled something quite so enticing before. He had to investigate. Though a few miles away he sniffed out your camp and watched for a while.

He didn’t really like the thought of humans in his territory but he stopped himself from attacking and scaring you all away because of you. Your smell was nice, sure, but now that he had seen you he knew he had to know more. You looked so delightfully fragile and soft. He wanted to watch you a bit longer before deciding what to do with you all.

Kurnugi observed you as you went about your tasks at the river. Delicate hands carefully tending to samples. He did not understand why you were doing it, but he liked the thought of those careful little human hands tending to his dick.

At first the scorpion man thought that perhaps the two large males with you were mates of yours but concluded that wasn’t the case as they never shared your nest, never physically touched you, and hardly verbally communicated with you. They seemed to just guard you and keep a lookout.

But they had been unable to spot Kurnugi given how well he stuck to the shadows, used sand to submerge himself partially, and carefully remained downwind of his quarry.

The longer he watched the more he realized how good a mate you would make. Too weak to ever oppose or escape him and he could tell by the way you collected and analyzed your little samples that you must be very intelligent. And you were just so much softer than anything he had ever encountered in this rugged environment. Surely if you were in a place as brutal as this then you’d need much better and dedicated protection than the ones you seemed to call Whitley and Matthews.

Kurnugi was much larger and stronger than any two humans, of course, no matter how well built they were. But they had long metal blades that may be able to get between his natural armor plates. Better to stalk your group for a while and wait for a moment of weakness.

He discreetly followed your party as you traveled away from your base camp and readied himself to attack when he noticed how tired all of you looked. Then the sandstorm struck.

Even a desert evolved being such as a mighty aqrabuamelu would not travel during a sandstorm.

When it passed he could tell by the scents he detected that your group was split up. This was even better. First he went after Matthews, then incapacitated Whitley before your very eyes. He had made sure not to kill them. No, they would surely be taken and bred by other desert creatures. There were many that loved humans.

And then there was you. It was cute how you thought you could escape from him so easily. He allowed you to try, watching in amusement as your tired chubby legs did their best to carry you away from him.

And then he rushed towards you, he closed the distance in surprisingly few seconds and stabbed your soft rear with the tip of his stinger. He was careful not to stab too deeply, but it was still pretty painful. You thrashed for a few seconds before crumpling to the sand.

The venom had paralyzed you, no matter how hard you tried you couldn’t get your limbs to cooperate at all. You could still feel everything though. The sand on your face, the tears about to burst from your eyes. You were certain death was what awaited you.

Instead the scorpion simply bent over to gently scoop you up in his humanoid arms. He held you close and buried his nose in your neck to revel in the intoxicating smell of his new mate, now finally in his possession.

The amorous aqrabuamelu rushed off to his den, holding you tightly to his hard body as he did so. He was excited to get you acclimated to your new home, and to his cock. He was sure after his displays of strength that he would have you moaning for his eggs in no time. Luckily his home wasn’t too far.

The entire trip he happily talked in a chittery voice to you, though you had absolutely no idea what he was saying. He sounded extremely excited though and rubbed your back soothingly as he spoke.

When he came to the entrance to his abode and scuttled into the cave in such a hurry that he almost tripped over himself. He placed you, all nice and paralyzed for him, on his raised pile of bedding and made short work of your clothing. Every inch of skin from your soft human breasts to your inviting pussy were laid bare under his hungry gaze.

He pulled you to the edge of his nest over to where he was standing. He gripped your thighs with his large hands and slowly spread your legs, as if unwrapping a precious gift. Savoring the moment before it was entirely open.

The venom was still coursing effectively through your veins, offering you no recourse. You could only stare into the terrifying red eyes of this abomination as he looked at you and drooled. His intent with you becoming all the more evident as his engorged cock protruded fully from the genital slit at the meeting of his scorpion and humanoid body segments.

There was no way something like that could enter a human without some degree of pain.

Seeing it you wanted to scream. To kick and fight. But you were a prisoner in your own body. All the struggle you could force out of your body were choked cries and grunts of fear.

The scorpion caressed your sides in a gesture meant to be comforting to his scared little mate, but any touch from him only brought about more terror.

He crouched down at the edge of the bed so that his head was level with your crotch. He inhaled the scent deeply before licking the outer folds of your sex.

"Mmmm~"

You could feel it in your entire body as he hummed loudly in contentment. Your flavor was better than he could have imagined.

Your cunt was wetter than it had ever been before. A fact that made you all the more frustrated and angry at yourself. It was bad enough you couldn't get your body to move.

Kurnugi was making out with your entrance, gripping your hips painfully and drawing blood as he lost himself in thoughts about you carrying his spawn.

He enjoyed every heavenly drop of your juices, mixing in a heavy amount of his saliva into you so that you were as lubricated as possible.

He decided you were as ready as you could be and rose up to press his cock against you.

Kurnugi rubbed the tip of his strange inhuman prick against your pussy, grinding against it. He let out a low moan as he plunged into you all at once, unable to hold himself back from doing so any longer. The paralytic you had been drugged with must have started to wear off by that point because as he shoved himself into you you were finally able to scream a bit and grip the bedding beneath you.

The length was insane but the worst part was the thickness. It was like it was trying to rip you open. He gave you time to adjust before he began his thrusting, but there was just no getting adjusted to that type of size in such a short amount of time without a lot more preparation.

You flinched and gasped in pain as he began slowly moving in and out of you.

The scent and sight of the bit of your blood that mixed with your fluids and smeared on his cock only seemed to excite him and he increased the pace, the outline of his cock plainly visible under the skin of your belly. He used one of his hands to rub at the outline as it moved back and forth inside of you, effectively giving himself a handjob while he bred you.

He lowered his head to your chest and ferally bit and licked at your breasts. Instinct demanding he marked and claimed what was, by all laws of the desert, his property.

After what seemed like an eternity you finally had acclimated to the size of the man taking you and began feeling less pain with flashes of pleasure. Chasing those brief flashes, you weakly grinded into each thrust from the scorpion.

Kurnigi’s roaming mouth had found your neck and was sucking and nipping at your soft skin there as if his life depended on it.

The stimulation was only feeling better as the two of you continued, your cries of fear and pain having long since been replaced by weak lusty moans and shivers of delight. You came a couple times and by the third you were completely fucked out. You had never orgasmed so hard in your life and were barely aware of anything other than that nice feeling between your shaky legs.

Kurnugi finally hit his climax as well and bit your neck hard as he did so, injecting something into your neck to leave a permanent claiming mark on you. You flinched in pain but it receded quickly, you were too out of it to really care what he did to you at that point.

Until you felt him cum into you. Your eyes went wide at the sensation as you felt relatively large round masses being pumped deeply into you.

Eggs.

You were shocked and disgusted, but there was nothing to be done about it then. You let the aqrabuamelu lick your various scratches and bite wounds clean before tucking you in with blankets so you could sleep while he made a meal.

He was so jubilant that his pregnant little mate was cozy in his nest while he took care of you. He had a nice home, a large territory, and now a soft mate to dote on and stuff full of his big prick whenever he needed to. Life was perfect.

THREE MONTHS LATER:

It had been around three months since you had been abducted. And it was hours since you had run away from the monstrosity that had kidnapped you. His name was Kurnugi, you had learned, managing very limited communication with the brute. He had finally let his guard down, finally trusted you since you had never been anything but compliant since you had been with him, and he had finally left you alone to go hunt for a longer period of time.

You did not waste the opportunity, it was clear that your bodyguards had not managed to leave the desert, probably succumbing to exposure. Either that or no one had cared to send a search party to look for you thinking you were a lost cause. So it was up to you to rescue yourself.

Once more your legs burned with a familiar exhaustion. Kurnugi was a skilled hunter that moved fast. Once he returned to his den he would begin tracking you immediately. If you were going to succeed then you had to get as much distance between yourself and that cave as you could possibly manage. It didn’t help that you were pregnant with his many eggs. It surely made travel more difficult.

As you made your way up the river you considered walking in it to hide your scent, but you decided against it. The wind was blowing your scent in the direction that you were fleeing from so it wouldn’t help you at all. The slog through the water would only slow you down. And that was the last thing that you needed right then.

The last thing other than suddenly finding yourself laying with your back against the hot sand and looking up with an absolutely outraged Kurnugi looking down at you with a malicious grin on his face.

How had he snuck up on you so silently?

You had expected to be immediately paralyzed by one of his stings, but the scorpion had other things in mind. He wasn’t just angry. He was hurt. You had tried to leave him the first opportunity you had. Had he not proven himself against lesser males to be a strong mate? Had he not provided food and water and shared his home with you? Had he not given you a nice belly full of eggs as any mate would desire?

The aqrabuamelu clearly had to show you your place. He had been too gentle with you. Not done enough to prove that you were, beyond any whisper of a doubt, his property.

And in that vein of thinking he did not bother with his venom, no, he wanted you to have the full ability of movement and still be just as utterly powerless to do anything to avoid him using you as he saw fit.

He pushed you over onto the ground and flipped you so that you were facing the sand with your ass up. Then, right in the middle of the desert, he ripped off the pants he had made for you out of leather and plant fibers.

Kurnugi crouched so that his cock was level with your cunt, his strong hands gripping your hips so hard that they would undoubtedly be bruised. You cried out and struggled just as pathetically as he had predicted that you would.

“Please! Please NO!!! I’ll behave! I-I’ll never leave again. KURNUGI PLEA-”

Your words became a pained shriek as he interrupted them by ramming his entire dick straight into you. No prep at all. He understood your cries for mercy and carried on anyway.

Each thrust into you was slow, powerful, and deliberate, driving your face into the sand as his nails bit into your flesh.

You were right about one thing. You’d never try to leave him again after this. He was going to fuck it into your brain so you’d have no chance of forgetting.

Tears dripped down your face, wicked away by the thirsty air and ground. Your legs shook and you would have collapsed were it not for the strong hands propping you up. Your abused cunt felt like it was being torn apart.

Kurnugi was once more excited by the scent of your blood and began pulling you back and forth over his prick like you were just a cock sleeve built for his pleasure. All you could do was sob and take it and try not to puke.

With a final slam he came in you hard. No eggs this time, since you were already full of them, just fluid that thankfully soothed your insides, if only a bit.

The anger of the aqrabuamelu was finally quelled and he panted with his throbbing cock still twitching and pumping cum into you. He picked you up and renewed the bite mark he had left on you. He did not have to since it was permanent, but he wanted to so you’d remember you were branded property.

Luckily the pain from the bite had been swallowed up by the disorientation and pain from the rape.

A small comfort.

Kurnugi picked you up effortlessly and cradled you close to him.

You couldn’t quite tell what he had said, but you knew it was meant to be soothing. He rubbed your back and chittered sweetly in that foreign language of his. Evidently happy to have that nasty business out of the way and content in the knowledge that you wouldn’t be trying that again.

7 MONTHS LATER:

You couldn’t wait for the eggs inside you to hatch. Kurnugi had assured you that you would be a great parent and since the eggs absorb the DNA of the parent they are deposited in he was confident that they would be the cutest babies in the entire world.

He was so wonderful to you. He doted on you and helped take you for walks when you were restless. He caught or foraged and then prepared all of your meals. He cuddled you close and kissed your swollen belly and made every effort to cheer you up when you were feeling down.

And you were sweet to him too, you learned how to craft things from the leather and fibers he made so you made him little trinkets that he either wore or decorated the den with. And you always greeted him by kissing him softly whenever he came home.

Things were a lot easier now that you could communicate.

And now that you understood that you could never go anywhere without him and to try to do so would be very hurtful for the both of you.

But why would you ever want to do something like that?

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[brunchable]

LAZARUS SERUM || Steve Rogers x Enhanced!FReader

Part III

Part One | Part Two | Final Words: 11.1K Themes: Angst, Drama, Violence, Action (Fighting Scenes: Steve vs Bucky), Hatred, Lovers to Enemies, Enemies to Lovers. Warning: Emotionally heavy? Death. Sneak Peak: Bucky’s eyes flicked to yours, and for a moment, he saw the vulnerability that you so rarely showed. “Stark’s a paranoid bastard, but he’s not wrong about everything,” he replied. “HYDRA always had a backup plan.”

You nodded, biting your lip as you turned back to the window. “I’ve got people waiting for us at my father’s old place. People who want to rebel. But if Stark’s right… if there’s something inside me that they can still control…”

A/N: This was difficult to write man, I feel like i keep repeating myself. Anyways this short story is almost done, I'm thinking to make one for bucky in this same timeline, but i dunno.

Tags: @realifelamb @needsleep3000 @vicmc624 @i-can-do-this-all-dayy @mrs-jjmaybank @strepsils123 @nesnejwritings @haruvalentine4321 @feelinthefic @niffala @fantasyfootballchampion @thefandomplace

SHIELD agents moved about the Helicarrier, carrying out their usual duties. The tension from your recent capture still lingered in the air, but for the moment, everything seemed normal.

Inside one of the briefing rooms, the Avengers gathered around a large, circular table. The holographic display in the center flickered to life. Steve stood at the far end, his expression remaining grim. He had been silent since his conversation with you, apart from when he told Fury about the new information about you.

Nick Fury stood at the head of the table, his one good eye sweeping over the assembled team. He cleared his throat, drawing everyone’s attention as the screen behind him shifted to show a detailed molecular structure—one that looked alien yet familiar.

“This,” Fury began, pointing to the hologram, “is the Lazarus Serum. It was an experimental project conducted by a rogue HYDRA scientist during World War II. What makes this serum different from the Super Soldier Serum is that it’s derived from an unknown foreign DNA, not of earth.”

The room fell into a stunned silence. Tony’s brow furrowed in thought, while Natasha leaned forward, her eyes narrowing at the image on the screen.

“So an Alien DNA?” Natasha echoed, disbelief evident in her voice. “How did HYDRA even get their hands on something like that?”

Fury’s expression darkened. “During the war, HYDRA had their hands in more than just earthly affairs. There were rumors that they made contact with otherworldly entities, collecting samples and conducting experiments. Most of these were failures or too dangerous to continue, but the Lazarus Serum was different. It worked, but with a catch. It enhanced the subject’s strength, speed, and durability—just like the Super Soldier Serum—but the side effects were severe: mental instability, increased aggression. . .making them unhinged.”

“And Y/N?” Steve’s voice was low, but it carried a weight that made everyone turn toward him.

“She’s the only known survivor of the Lazarus project,” Fury confirmed. “And because the serum is derived from Alien DNA, its effects are permanent. Y/N is more than just enhanced—she’s on a whole other level. The serum doesn’t just slow aging; it effectively makes her immune to most forms of injury. We’ve seen that she can take bullets without flinching, and that’s just the tip of the iceberg.”

Fury opened up a highly confidential video file named, ‘Lazarus Serum’ and instantly piqued the team's curiosity.

As the holographic footage played, the room fell into a tense silence. The screen displayed a black-and-white, grainy video of a sterile HYDRA laboratory, dimly lit and cold, with the camera focusing on you, strapped to a metal table. The tension among the Avengers was palpable as they watched, the atmosphere heavy with the gravity of what they were about to witness.

You lay on the table, your body weak and trembling. The serum coursing through your veins was taking its final toll, pushing you to the brink of death. Your skin was pale, your breaths shallow, and your heartbeat was erratic, the monitors showing your vitals dipping dangerously low.

Fury’s voice cut through the footage, steady but grim. “This is what our talented hackers are able to salvage, the final recorded moment of the Lazarus Serum experiment. Day 100.”

On the screen, the lead scientist stood over you, his expression hard as he observed the monitors. Despite the clear signs that you were dying, he was refusing to back down. The technician beside him looked increasingly anxious, glancing between the monitors and your convulsing body.

"Her vitals are deteriorating rapidly," the technician warned, his voice shaking. "She's not stabilizing. We should stop."

The scientist’s jaw tightened, his eyes narrowing with determination. "We’re too close. Increase the dosage."

"But sir, she won't survive—"

"Do it!" he barked, silencing any further protest.

The technician hesitated but ultimately complied, injecting you with another dose of the glowing serum. The screen showed your body convulsing violently on the table, the restraints biting into your skin as your body tried to fight the unbearable pain. The lines on the monitors went haywire, your heart rate spiking uncontrollably before it suddenly flatlined.

Steve couldn’t bear to watch any longer. The sight of you, broken and tortured. His heart clenched with a pain so intense it might as well be a heart attack. It was clear that every second of this footage was torture for him, he tried to keep watching but turned his head away, unable to watch life being drained from you. His fists clenched at his sides, his knuckles white as he fought back the tears threatening to spill over. He couldn’t bring himself to see the moment you died, the moment HYDRA took everything from you.

Fury paused the footage briefly, his gaze sweeping over the team. “At this point, HYDRA pronounced her dead. The lead scientist, as you can hear, said to ‘Dispose of the body.’ They believed the experiment was a failure, and that Y/N had died.”

The footage resumed, showing the scientist’s callous order to dispose of your body. The camera caught the technician’s expression—a mix of shock and fear—as he moved to unstrap your lifeless form from the table. The filming stops and the screens go black.

“We can only guess what happened next,” Fury’s voice echoed through the room. “This is why it’s called the Lazarus Serum. Like the Lazarus of myth, it brought her back to life. The woman you knew, Steve, died on that table. What came back was something else.”

“Excuse me.” Steve quietly excused himself from the room, his thoughts too heavy to focus on a strategy. He needed a moment alone, to process what he had just seen, and to prepare himself for the fight of his life.

Tony, who had been deep in thought, glanced up just in time to see Steve slipping out of the door. Something about the way Steve moved—so rigid, so controlled—set off alarm bells in Tony’s mind.

“Hey, Cap—” Tony called after him, but Steve didn’t stop. He didn’t even turn his head. He just kept walking, his footsteps echoing down the corridor.

Tony’s eyes narrowed as he watched Steve go. He knew that look, the one where Steve was bottling everything up inside, pretending he was fine when he was anything but.

× × × × 

Steve barely made it to his quarters before the dam broke. The door slid shut behind him with a soft hiss, sealing him in the solitude he so desperately needed. For a moment, he stood there, his back against the door, his breath coming in short, sharp gasps as he fought to maintain control.

But the weight of everything he had just seen and heard—the memories, the footage of your death, the knowledge that you had suffered because of him—crushed down on him with a force that made his knees buckle. He stumbled forward, his vision blurring with unshed tears.

With a choked sob, Steve lashed out, his fist slamming into the nearest object—his desk. The wood splintered under the force of the blow, but the pain in his knuckles barely registered. He hit it again, and again, each impact sending shockwaves up his arm, but it wasn’t enough. It would never be enough.

He threw a chair, shattered a picture frame, and ripped the curtains from the window, the fabric tearing with a violent snap. His breath came in ragged gasps, his chest heaving as he struggled to breathe through the crushing weight of his emotions.

The door to his room suddenly opened, and Tony Stark stepped inside, his usual confident demeanor faltering as he took in the sight of Steve in the midst of his rampage. Steve didn’t notice him at first, too lost in his own agony to register anything beyond the need to destroy, to somehow externalize the pain that was tearing him apart from the inside.

“Steve!” Tony’s voice was sharp, cutting through the chaos. But Steve didn’t stop. He grabbed the closest thing within reach—a lamp—and hurled it across the room, the glass shattering as it struck the wall.

“Steve!” Tony shouted again, moving quickly to grab Steve’s arm as he reached for another object. Steve yanked his arm away, his eyes wild and unfocused, his chest heaving as he glared at Tony.

“Get out!” Steve’s voice was raw, torn from the depths of his soul. But Tony didn’t back down. Instead, he reached out again, this time catching Steve’s wrist in a firm grip.

“Steve, stop!” Tony’s voice was filled with a mixture of urgency and compassion as he tightened his hold. “You’re going to hurt yourself!”

Steve struggled but held back in Tony’s grip, his knuckles already bleeding from the impacts against the wooden furniture. But Tony didn’t let go. He could see the pain in Steve’s eyes, the anguish that was tearing him apart, and he knew he couldn’t just leave him like this.

“Damn it, Rogers, look at me!” Tony’s voice cracked with emotion as he forced Steve to meet his gaze. “You can’t keep doing this to yourself!”

For a moment, Steve’s rage faltered, his eyes locking with Tony’s. The room fell into silence, the only sound the ragged breathing of the two men standing in the midst of the wreckage. And then, as if the fight had been drained out of him, Steve collapsed to his knees, his body trembling with the force of his sobs.

“I did this,” Steve gasped, his voice choking with emotion. “All of this.”

Tony knelt beside him, his heart aching at the sight of his friend so completely broken. He pulled Steve into a tight embrace, holding him as Steve’s walls crumbled completely.

Steve fought against it at first, his body stiffening as if trying to hold onto the last shreds of his composure, but eventually, he couldn’t resist. He rested his forehead on Tony’s shoulder, his sobs coming harder and faster, his breath hitching in his throat.

“You can’t blame yourself for everything,” Tony whispered, his own voice thick with emotion as he acted as Steve’s rock. 

“I failed,” Steve cried, his voice muffled against Tony’s shoulder. “I left her when she needed me the most. How can I ever make that right?”

“Damn it,” Tony mumbled, tightened his grip, his own throat constricting as he tried to hold back his own tears, “HYDRA did this, not you. There is always a way.”

× × × × 

Tony entered the cell, his expression devoid of the usual levity. He took a moment to observe you, noticing the way your eyes followed his every move, calculating, waiting.

"Back so soon, Stark?" You broke the silence, your voice a smooth mixture of mockery and disinterest. "Did you miss me?"

Tony didn't rise to the bait. Instead, he pulled out the chair across from you and sat down, folding his hands in front of him. He stared at you for a long moment, his gaze unflinching.

"I think you and I both know there's more to this Lazarus Serum than you're letting on," Tony began, his tone conversational but with an edge of steel beneath it. 

“I see Rogers didn't waste any time, after knowing about my serum,” you chuckled and raised an eyebrow, a hint of amusement flickering in your eyes. "Is this the part where you try to psychoanalyze me? Try to figure out what makes me tick?"

"Oh, I don't need to figure out what makes you tick. I already know. You’re angry, bitter, and probably more than a little scared. Not that you’d ever admit it." Tony leaned back in his chair, crossing his arms casually.

Your eyes narrowed slightly, the first crack in your carefully maintained facade. "You don’t know anything about me."

"Don’t I?" Tony replied smoothly, his voice taking on a tone of calculated sympathy. "You were Steve’s girl, right? The one who got left behind, forgotten. You were just a kid when HYDRA took you, and they twisted you into something… else. You’re not angry because of what they did to you. You’re angry because the people who were supposed to care about you—people like Steve—didn’t save you."

Your hands clenched into fists at Tony’s words, the knuckles turning white. "You think you’re clever, now huh? You think you can come in here and play mind games with me?"

Tony leaned forward, his eyes locking onto yours with an intensity that was hard to ignore. "I’m not playing games. I just want to understand what’s going on here. Because if there’s one thing I’ve learned, it’s that nothing is ever as simple as it seems. You hate Steve, sure. You hate me, too, probably. But I’m betting there’s a part of you that hates HYDRA even more. So why don’t you tell me what they really did to you? What’s the catch with this Lazarus Serum? What are they not telling you?”

You held Tony's gaze, the flicker of something darker passing through your eyes. But you kept your expression as neutral as you could. "You think I’m just going to spill my secrets to you? You’re more naive than I thought."

Tony smirked, leaning back again, feigning a relaxed demeanor. "Oh, I don't expect you to spill everything. Not right away. But I’m pretty good at reading between the lines. And right now, you’re giving off a lot of signals, sweetheart."

The nickname was meant to annoy you, but you didn’t react, keeping your face impassive. Instead, you decided to toy with him a bit. "You really think you’re different from the rest of them, don’t you? Just because you’re a genius in a tin suit? HYDRA, SHIELD, you—they’re all the same. You all think you’re the smartest one in the room. But you’re all playing the same game. You’re all just cogs in the same machine."

Tony’s eyes narrowed slightly, but he kept his cool. "That’s a nice speech. But I’ve been in your shoes, remember? Taken apart, piece by piece, rebuilt by people who didn’t give a damn about what they were doing to me. So why don’t we cut the crap? What did HYDRA really do to you?"

You leaned forward, matching his intensity. "They made me better. Stronger. Faster. Everything your buddy Rogers has, and then some. But you already know that. What you really want to know is how to stop it."

Tony tilted his head, a small smile playing on his lips. "So it’s true then? There’s no off-switch? No failsafe? You’re just a ticking time bomb, aren’t you?"

Your eyes flashed with anger, and for a moment, you almost let it slip. Almost. But you caught yourself, settling back in your chair with a cold smile. "Nice try. But you’re not as clever as you think you are."

Tony’s smile didn’t falter, but his eyes hardened. "You’re right. I’m not trying to be clever. I’m trying to help you. You think you’re untouchable now, but the truth is, HYDRA doesn’t make anything without a leash. You’re telling me there’s no failsafe? No way to control you if you step out of line?"

You didn’t respond immediately, and that was all Tony needed. "See? That, right there. You hesitated. There’s something you’re not telling me. Something even you don’t know."

You clenched your jaw, the silence between you thickening. Tony was getting under your skin, and he knew it. He leaned in closer, his voice dropping to a whisper. "You think you’re free? You’re just as much a prisoner as you were when they first strapped you to that table. And deep down, you know it."

"You think you can manipulate me with your cheap psychology tricks? I’ve had experts try to break me, Stark. You’re not even in the same league."

Tony’s expression remained unfazed. "I don’t need to break you. I just need to understand. Because if you think for one second that I’m going to let HYDRA have their way with you—or with anyone else—you’re sorely mistaken."

You stared at him, your mind racing. Tony Stark was a lot of things, but he wasn’t stupid. And he was right about one thing: HYDRA didn’t make anything without a failsafe. But what did that mean for you? Did you even want to know?

Tony watched the conflict play out on your face, and he decided to push just a little bit harder. "You can keep pretending that you’re in control, that you’re the one calling the shots. But at the end of the day, you’re just another one of HYDRA’s experiments. And when they’re done with you, they’ll toss you aside like garbage."

Your hands trembled slightly, the anger boiling just beneath the surface. "Shut up, Stark."

He leaned back, satisfied that he had struck a nerve. "We’ll talk again when you’re ready to stop lying to yourself. And trust me, you will."

As Tony stood up to leave, you couldn’t help but wonder if he was right. The idea that HYDRA could still have control over you, that there was something they hadn’t told you—it gnawed at you. But you couldn’t let him see that. You couldn’t let him win.

Just before Tony reached the door, you spoke again, your voice cold and distant. "You think you know everything now. But it's only the tip of a needle."

Tony paused, turning back to you with a raised eyebrow. "Then enlighten me."

But you didn’t say another word. You simply stared at him, your eyes burning with a mixture of anger and fear. Tony shook his head slightly, a hint of disappointment in his expression before walking out and leaving you alone with your thoughts.

And for the first time in a long time, those thoughts weren’t comforting.

× × × × 

Your eyes snapped open. The blaring sound reverberated through the metal structure of the Helicarrier, signaling chaos above. A slow, cold smile spread across your lips, a glimmer of dark satisfaction in your eyes.

Leaning back against the wall, you tilted your head slightly, listening to the escalating panic beyond the cell door. The symphony of chaos was almost... delightful.

“Finally,” you whispered to yourself, your voice barely audible over the din.

The lights flickered, the warning sirens growing louder and more insistent as SHIELD agents scrambled to their stations. The Avengers, who had been spread out across the ship, immediately converged in the command center, each of them on high alert.

"What now?" Tony muttered, his face twisting into a frown as he glanced at the flashing red lights. 

Fury's voice boomed over the intercom. "We've got hostiles onboard. This isn't a drill—The Winter Soldier and a squad of HYDRA operatives just breached the perimeter." 

Steve's heart plummeted. He had just started to gather himself after the emotional breakdown of learning about your past and his rage-fueled outburst. Now, hearing about Bucky’s presence, he felt a fresh surge of desperation. He had to stop him from reaching you.

As they ran through the Helicarrier's corridors, the sounds of battle grew louder. Gunfire echoed off the metal walls, and the smell of smoke and burning electronics filled the air.

The Helicarrier shook as explosions ripped through the structure, sending sparks and chunks of metal cascading through the corridors. 

Alarms blared, drowning out the shouts of SHIELD agents and the staccato of gunfire. The team raced through the chaos, dodging falling debris and returning fire at HYDRA agents who revealed themselves.

Natasha sprinted ahead, her movements fluid as she flipped over a barrage of bullets, landing gracefully before taking out two HYDRA agents with precise shots to their heads. She quickly ducked behind cover, reloading her pistols, eyes scanning for the next threat. Clint, perched in a higher vantage point, loosed arrows with deadly accuracy, each one finding its mark in the chest or throat of a HYDRA agent. 

“We’ve got to cut them off before they reach Y/N!” Steve shouted through the comms, his voice barely audible over the chaos.

Tony took to the air, his repulsors humming as he blasted through a wall to create a shortcut. 

“On it!” he called out, targeting a group of HYDRA agents laying down suppressive fire from a balcony. His repulsors lit up the darkened corridor as he unleashed a barrage of energy beams, reducing the balcony—and the agents on it—to rubble.

Meanwhile, Steve charged forward, his shield a blur of motion as he deflected bullets and took down any HYDRA operatives in his path. He was relentless, driven by the singular focus of reaching you before it was too late. His heart pounded in his chest, not just from the exertion of battle, but from the gnawing fear of what would happen if Bucky succeeded.

Ahead of him, the Winter Soldier, was a force of nature. He moved with deadly precision, his metal arm crushing through walls and doors as if they were paper. With every obstacle he encountered, he either barreled through or dismantled it.

A squad of SHIELD agents attempted to block his path, but they were no match for his ruthlessness. Bucky dispatched them brutally, his expression unchanging as he advanced toward the detention level.

Steve caught sight of Bucky just as he took out the last of the SHIELD agents guarding the entrance to your cell. 

“Bucky, stop!” Steve shouted, his voice carrying through the smoke-filled corridor.

Bucky turned slowly, his eyes narrowing as he locked onto Steve. There was a flicker of recognition, but it was quickly buried beneath the cold, mechanical focus of the Winter Soldier. Without a word, Bucky charged at Steve, their collision echoing through the Helicarrier like a thunderclap. The force of their impact sent shockwaves through the surrounding walls, cracking the metal and sending a rain of sparks from the overhead lights.

The fight was immediate and brutal, each man fighting with a desperate intensity that went beyond the mission. Steve knew he had to stop Bucky, not just to protect you, but to save the man who had once been his best friend. But Bucky was relentless, his movements precise and deadly, every strike aimed to incapacitate or kill.

Steve blocked a powerful punch from Bucky’s metal arm with his shield, the impact reverberating up his arm and nearly knocking him off balance. He retaliated with a swift kick to Bucky’s midsection, but Bucky barely flinched, grabbing Steve by the arm and slamming him against the wall with enough force to dent the metal.

“You don’t have to do this!” Steve shouted, ducking under a wild swing from Bucky’s left fist. “You’re James Buchanan Barnes! You were born in Brooklyn, 1917! We grew up together—best friends!”

“Shut up!” Bucky growled, his voice low and dangerous as he swung his metal arm at Steve with murderous intent. “I don’t want to hear it!”

But Steve wouldn’t stop. He couldn’t. “You’re Bucky! You joined the 107th Infantry, you fought in the war, you saved my life more times than I can count!”

“Shut up!” Bucky roared, slamming Steve into the wall again, the force of the impact cracking the metal. “Shut up!”

But Steve pressed on, his voice cracking with desperation. “You’re a brother to me, Bucky! We went through hell together! You’re not this—this weapon HYDRA made you into! You’re James Buchanan Barnes!”

Bucky’s eyes flashed with something dark and twisted as he Bucky’s grip tightened around Steve’s throat, the rage in his eyes burning brighter with each word Steve spoke. “I told you to shut up!”

But Steve, despite the choking pressure on his windpipe, forced the words out. “You’re... Bucky... you’re... my brother...”

With a snarl, Bucky shoved Steve back, releasing his grip only to drive a brutal punch into Steve’s midsection. Steve doubled over, gasping for air, but even as he staggered, he refused to give up.

“Remember... who you are, who I am,” Steve rasped, clutching at his ribs as he straightened up. 

"I don't care to remember who you are but I do remember this. . .”

Steve’s heart surged with a flicker of hope, but it was quickly dashed as Bucky continued, his tone dripping with malice. “I remember the way she used to scream for me, the way she begged because someone couldn't satisfy her.”

Steve saw red.

Steve launched himself at Bucky, his fists flying with a ferocity he hadn’t unleashed in years. All the restraint, all the control he usually maintained, evaporated in the face of Bucky’s vile taunts. He swung his shield with all vicious force, slamming it into Bucky’s side and following up with a brutal uppercut that sent Bucky staggering back.

But Bucky recovered quickly, his expression cold and unyielding as he blocked Steve’s next punch with his metal arm. The force of their strikes echoed through the corridor, each blow landing with the weight of their shared history and the emotional wounds that had been ripped open.

“You don’t know what you’re talking about!” Steve shouted, his voice hoarse with rage as he slammed his shield into Bucky’s midsection again. “This isn’t you! HYDRA did this to you! You’re not their puppet!”

But Bucky just sneered, his eyes filled with contempt as he caught Steve’s next punch and twisted his arm painfully. “She never begged you for more, did she? No matter how much you tried, she always needed me to finish what you couldn’t start.”

The taunt was the final straw. Steve’s vision blurred with a red haze as he let out a guttural scream, wrenching his arm free and slamming his shield into Bucky’s chest with enough force to crack the metal floor beneath them. He didn’t care about strategy or technique anymore—he just wanted to hurt Bucky, to make him pay for every word, every twisted memory HYDRA had implanted in his mind.

Steve pressed his advantage, driving Bucky back with a flurry of powerful strikes. He landed a solid punch to Bucky’s jaw, then followed it up with a brutal shield bash that sent Bucky staggering. For a moment, it seemed as though Steve might actually overpower him. Bucky’s defenses faltered, and Steve took full advantage, slamming him into the wall with a force that made the entire corridor shudder.

“You don’t get to talk about her!” Steve roared, his shield coming down like a hammer, striking Bucky again and again. The fury in his eyes was all-consuming, his movements fueled by a deep, protective rage.

Bucky grunted in pain, the relentless assault forcing him onto the defensive. He blocked a few of Steve’s strikes, but the sheer force behind each blow drove him back, his metal arm straining under the impact.

“You’re not taking her!” Steve shouted, driving his knee into Bucky’s midsection and following it with a devastating uppercut that sent Bucky crashing to the ground.

For a moment, Steve loomed over Bucky, panting heavily, his eyes blazing with anger. He had the upper hand, and Bucky looked up at him with something that might have been fear or perhaps something darker.

But Bucky wasn’t finished. As Steve moved in for the final blow, Bucky’s eyes narrowed, and with a sudden, vicious twist, he swept Steve’s legs out from under him. Steve hit the ground hard, his shield clattering to the side. Before he could recover, Bucky was on him, his metal arm clamping around Steve’s throat, squeezing with unyielding force.

Steve’s vision darkened as he struggled against Bucky’s grip, the initial surge of rage drained him, leaving him vulnerable. Bucky leaned in close, his expression cold and unforgiving, his grip tightening as if to finish him off.

But then, something shifted in Bucky’s eyes. His grip faltered, the anger in his expression wavering. He stared down at Steve, breathing heavily, and for a moment, he looked... lost. The hostility that had driven him was still there, but it was mingled with familiarity.

Steve, gasping for air, could see the hesitation in Bucky’s eyes. “You...will put her in danger if you take her,” he managed to choke out, his voice strained but firm. “You’re more than what they made you.”

Bucky’s hand trembled, the pressure on Steve’s throat easing slightly. He didn’t know why, but the idea of finishing Steve off suddenly felt wrong. There was a nagging feeling deep inside him, something that pushed against the cold, mechanical orders HYDRA had drilled into him.

He released his grip on Steve’s throat entirely, stepping back as if unsure of what he had been about to do. He looked down at Steve, who lay on the ground, coughing and trying to catch his breath, and for the first time in a long time, Bucky felt a pang of guilt.

“I... don’t care who you are,” Bucky muttered, his voice cracking with an emotion he couldn’t place. “But stay out of my way.”

Instead of delivering the final blow, Bucky turned and walked away, leaving Steve lying on the cold metal floor, bruised and battered but still conscious.

Bucky reached the detention level, his pace quickening as he neared the cell where you were held. The door loomed ahead and he entered the pin that he was given by one of the agents.

He stepped into the cell, his expression softening as he saw you. “Let's go,” he said, his voice filled with a protective tone that left no room for doubt. It didn’t matter who stood in his way—he would protect you at any cost, even if it meant defying the part of him that still hesitated, still questioned, still remembered.

× × × × 

In the hangar bay, Tony arrived just in time to see the transport vanish into the distance. He cursed under his breath, frustration and anger bubbling up inside him as he activated his comms.

“Fury, we’ve lost them. They’re gone,” Tony said, his voice laced with bitterness.

There was a brief pause on the other end before Fury responded. “Get Rogers and regroup. We’ll figure out our next move, but this isn’t over. Not by a long shot.”

Tony glanced back at the corridor where Steve was trying to get up with a grimace.

He made his way back to Steve, kneeling beside his fallen teammate. “Come on, Cap,” Tony muttered, “We’ve got work to do.”

Steve groaned as got up to his feet, head pounding from the beating he’d taken. The first thing he saw when he looked up was Tony’s face, his expression grim but not without a hint of concern.

“Y/N…?” Steve murmured, his voice weak as he tried to push himself up.

“They got her,” Tony replied, his tone flat. “Bucky took her, and they’re long gone by now.”

Steve’s heart sank, the weight of his failure crashing down on him like a ton of bricks adding to the pile. But Tony wasn’t about to let him wallow in self-pity.

“Save it for later, Cap,” Tony said, helping him to his feet. “Right now, we need to get back to the others. Fury’s not going to be happy about this, but we need to regroup and figure out our next move.”

Steve nodded numbly, still trying to process everything that had happened. The pain in his ribs and the bruises covering his body were nothing compared to the agony of knowing that he had failed again.

× × × ×

The dust began to settle, and the team regrouped, battered but unbowed. The realization of what had just happened—of how deeply HYDRA had infiltrated SHIELD—hung over them like a dark cloud. The Helicarrier was in disarray, corridors filled with debris, smoke still curling from shattered panels, and the distant sound of alarms echoing through the vast structure. SHIELD agents, those who remained loyal, were helping the wounded and trying to restore some semblance of order. 

Steve Rogers leaned heavily against a wall, his injuries and the emotional toll pressing down on him like never before. 

Tony Stark, always one to keep moving even when his mind was in chaos, was pacing back and forth, his suit clanking with every step. He had been replaying the events of the past few hours in his mind, analyzing every detail, every word, every move, searching for something they had missed. As his thoughts churned, a memory suddenly clicked into place, causing him to stop dead in his tracks, his eyes widening.

“Dammit,” Tony muttered under his breath, his voice tinged with frustration and a hint of disbelief. The suddenness of his outburst caused the others to turn toward him, their expressions questioning.

“What is it?” Natasha asked, her tone sharp as she watched Tony with narrowed eyes. She was on edge, the adrenaline of the fight still pumping through her veins, and the last thing she needed was another surprise.

Tony turned to face the group, frustration evident as he pieced it together out loud. “Y/N knew. That cryptic crap she said during the interrogation—it wasn’t just her messing with us. She was trying to tell us something.”

Steve straightened at the mention of your name, his heart tightening as he remembered the cold, distant look in your eyes during the interrogation. 

“What are you talking about, Tony?” he asked, his voice rough with exhaustion.

“She said something about how ‘keep your friends close. . . But your enemies closer,’ and that ‘you’d be shocked at how deep the rot goes.’ At the time, it sounded like she was just spewing nonsense to get under our skin. But now…it makes sense.”

“She was warning us,” Tony continued, his tone filled with a mix of frustration and reluctant admiration. “She was trying to tell us that HYDRA had already infiltrated SHIELD. She practically spelled it out for us, but we were too blind to see it. We were so focused on her as the enemy that we didn’t stop to think about what she was really saying.”

Natasha frowned, her mind racing as she recalled the conversation. “Why didn’t she just come out and say it directly?” she questioned, but even as the words left her mouth, she knew the answer. 

“Because she couldn’t,” Clint interjected, “If she’d said it outright, they’d know. HYDRA would have seen it, and they’d have to shut her down before she could give us anything.”

Steve, who had been standing off to the side, consumed with thoughts of you, felt a fresh wave of guilt wash over him. He had been so focused on what you had become—so fixated on the idea of saving you—that he hadn’t seen what you were trying to tell them. You had been trying to help them, in your own way, and he had missed it.

“Y/N was trying to help us,” Steve murmured, his voice filled with a mix of realization and sorrow. “But we didn’t listen.”

Tony’s expression softened slightly, though his usual bravado was still present. “She was still playing her own game, Cap. Don’t romanticize it too much. But yeah, she threw us a bone. And we missed it.”

Natasha stepped closer, her gaze firm and unyielding. “So what do we do now? We can’t trust anyone. HYDRA could be anywhere—everywhere. We need a plan.”

Steve’s mind was spinning, this was the last thing they needed. The fight with Bucky, the realization that you had tried to warn them, the knowledge that HYDRA had already sunk its claws deep into SHIELD—it was hard to believe, but that was now the reality.

But he couldn’t afford to falter. Not now. Not when so much was at stake.

“We need to figure out who we can trust, and take the fight to HYDRA. We’ll find Y/N and Bucky, and we’ll put an end to this.” Steve said firmly.

The others nodded. 

They had been blindsided, caught off guard by the depth of HYDRA’s infiltration. But they were the Avengers—they had faced impossible odds before, and they would do it again.

As the team moved to regroup and plan their next steps, Tony lingered for a moment, his gaze fixed on Steve. “Cap,” he said quietly, drawing Steve’s attention. “We’ll get them back. Both of them.”

× × × ×

The stolen Quinjet’s engines was the only sound between you and Bucky as the landscape blurred beneath. Outside, the world rushed by in a silent blur of greens and browns, but inside, the atmosphere was thick with uncertainty.

You sat in the copilot’s seat, staring out of the window, mind a whirlwind of thoughts. Tony’s words echoed in your head, a gnawing doubt that you couldn’t shake. You’re just a ticking time bomb, aren’t you? You remembered him saying, his voice laced with the same arrogance that always grated on your nerves. But this time, beneath the bravado, there was something else—truth.

Was there something HYDRA hadn’t told you? Some failsafe, hidden deep within you? The thought made you uneasy in a way you hadn’t felt in a long time. You clenched your fists, your knuckles turning white as you fought to keep your composure. You had always prided yourself on being in control, on being the one to pull the strings. But now… now you weren't so sure.

Beside you, Bucky was equally lost in thought, his eyes fixed on the controls, though his mind was elsewhere. Steve’s words from their brutal fight were like daggers in his mind, each one striking a different nerve. He had tried to shut them out, to focus on the mission, but they kept coming back, louder and louder. “You’re James Buchanan Barnes! You were born in Brooklyn, 1917! We grew up together—best friends!”

The memories were there, just out of reach, like a puzzle with pieces that didn’t quite fit. Bucky’s grip on the controls tightened as he fought the urge to scream, to give in to the frustration that was building inside him. But he couldn’t.

“We’re still quite far,” You said quietly, breaking the silence. Your voice was steady, but there was a tension beneath it that Bucky didn’t miss.

He nodded, glancing over at you. “You sure about this?” he asked, his voice gruff.

You didn’t answer right away, your gaze still fixed on the horizon. When you finally spoke, your voice was low, almost as if you were speaking to yourself. “I don’t know anymore. About anything.”

Bucky didn’t press you. He understood that feeling all too well. For the longest time, his life had been nothing but a series of orders, a mission without end. Now, here he was, flying towards a past that he barely remembered, with a woman who was just as lost as he was.

“Stark is right, you know,” you said suddenly, turning to face him. “About HYDRA. About the control they still have over me. Stark… Stark said something that’s been eating away at me. He thinks there’s a failsafe. Something I don’t know about.”

Bucky’s eyes flicked to yours, and for a moment, he saw the vulnerability that you so rarely showed. “Stark’s a paranoid bastard, but he’s not wrong about everything,” he replied. “HYDRA always had a backup plan.”

You nodded, biting your lip as you turned back to the window. “I’ve got people waiting for us at my father’s old place. People who want to rebel. But if Stark’s right… if there’s something inside me that they can still control…”

“Then we deal with it,” Bucky said firmly, his voice cutting through your doubt. “We’ll find out what HYDRA did, and we’ll tear it out by the roots.”

You glanced over at Bucky, a serious expression settling over your face. “Now that my plan to sabotage Pierce succeeded, he has new enemies,” you said, your voice steady and sure. “What’s left of SHIELD… and us. I just know he's scrambling to get the upper hand.”

Bucky paused, taking in your words before he asked the question that had been nagging at him. “Why did you help them? The Avengers.”

Your gaze shifted back to the window, your expression hardening slightly. “I didn’t.”

Bucky’s brow furrowed, confused. “What would you call it then?”

There was a moment of silence, heavy with the weight of what lay unspoken between you. Finally, you turned to face him, your eyes sharp and unyielding. “Taking down Pierce. That was my only goal. The Avengers were just… a means to an end.”

Bucky studied you, searching for any hint of hesitation or doubt. “So you used them?”

“Use them, manipulate them, call it what you want,” you replied, your tone firm but devoid of the venom that usually laced your words. “I needed them to believe they had the upper hand. It was the only way, very hypocritical. I know.”

Bucky nodded slowly, processing your words. He could see the logic in it, but there was something in your tone, in the way your eyes darkened when you spoke, that told him there was more to it than you were letting on.

“Do you regret it?” he asked quietly.

You didn’t answer immediately. Your gaze drifted back to the horizon, your expression unreadable. 

“Regret is a luxury I can’t afford, Bucky,” you said finally. “Pierce needs to fall, and I did what I had to do to make that happen, for your freedom.”

“And yours. . .?” he asked, his voice quiet but insistent. 

You stayed silent, your expression unchanged, but the slight tightening of your grip on the armrest didn’t escape Bucky’s notice. The question hung in the air, unanswered, and he realized that perhaps even you didn’t know the answer.

× × × × 

The next day.

The Quinjet landed softly in the clearing, its engines whirring down to a low hum as the dust settled around it. You stepped out first, scanning the familiar surroundings of your father’s old home. It was a place of memories, both comforting and haunting. The farmhouse had weathered time well, standing as a symbol of resilience, much like those who now sought refuge within its walls.

Bucky followed close behind, his eyes sweeping over the perimeter, ever vigilant. As they approached the entrance, one of your  trusted agents—Agent Lawson—emerged from the shadows, his face grim.

“Welcome back, ma’am,” Lawson said, his tone respectful but tinged with urgency. “We’ve got intel. You need to see this.”

Your exchanged a brief glance with Bucky before nodding to Lawson. “Inside,” you said, your voice steady despite the unease growing within you.

You entered the farmhouse, the interior dimly lit but secure. The agent led them to a small room that had been converted into a makeshift command center. Maps, monitors, and files were spread across a large table, each detailing various aspects of their ongoing struggle against HYDRA.

Lawson handed you a tablet, his expression serious. “Ma’am, the Avengers went public with the exposure of HYDRA. It’s all over the news now. HYDRA’s operations within SHIELD are out in the open, and they’re scrambling.”

Your eyes narrowed as you took the tablet, the screen lighting up with a news feed. On it, you could see footage of Captain America, Steve Rogers, speaking directly to the camera in a broadcast that had gone out worldwide. But as you listened to his words, you quickly realized there was something that felt intimately directed at you.

“If you launch those helicarriers today, HYDRA will be able to kill anyone that stands in their way, unless we stop them,” Steve’s voice echoed through the room, his tone calm but behind it, something deeper—something only you would recognize. “I know I’m asking a lot, but the price of freedom is high; it always has been.”

Steve’s voice softened just slightly, a subtle change that might go unnoticed by most, but not by you. “And it’s a price I’m willing to pay,” he continued, his words lingering, as if speaking directly to you, “I’m not afraid to fight for what matters… or who matters.”

Your heart clenched at the unmistakable message, meant for you and you alone. “If I’m the only one, so be it. But I’m willing to bet I’m not.”

The screen froze on Steve’s face, his expression resolute, but his eyes—those eyes that you knew so well— carried a message meant just for you.

“They’ve dealt HYDRA a major blow, but Pierce is still out there,” Lawson continued, oblivious to the personal significance of Steve’s words. “He’s regrouping, and he’s made it clear that he’s coming after you, ma’am.”

You stared at the frozen image of Steve for a moment longer, your emotions swirling, threatening to break through the carefully constructed walls you had built around yourself. But then, with a sharp inhale, you forced those emotions down, locking them away where they couldn’t distract you.

Your face hardened as you shoved the tablet back into Lawson’s hands, your voice clipped and cold. “What’s Pierce planning?”

Lawson, slightly taken aback by your sudden change in demeanor, quickly handed you a folder. “We intercepted some communications from what’s left of HYDRA’s network. Pierce is regrouping, but he’s been severely compromised.”

You opened the folder, your eyes quickly scanning the contents. As you read, your expression darkened further. “What’s his next move?” you asked, your tone flat, all traces of emotion wiped clean.

Lawson hesitated, his eyes flickering to Bucky before he spoke. “He’s going after you, ma’am. Pierce knows he’s lost control of HYDRA’s influence within SHIELD, but he’s not done yet. He’s activated something—something called Project Lazarus.”

“That bastard Stark was right,” You laughed but your grip on the folder tightened.

“He’s desperate,” you said quietly, your eyes narrowing as you pieced it together. “If he can’t control me, he’ll try to eliminate me. He’s betting everything on this failsafe.”

Lawson nodded. “We believe he’s mobilizing the remnants of HYDRA to locate and retrieve whatever information or technology is needed to activate it. If he gets his hands on it, you’ll be his primary target.”

“We need to find out exactly what and where this failsafe is and how to disable it.” you closed the folder with a snap, your expression cold and resolute. “Lawson, I want every available resource focused on this. Find out everything you can about Project Lazarus—what it is, where it’s stored, how it works. We don’t have much time.”

“Yes, ma’am,” Lawson said, his voice firm as he prepared to carry out your orders.

Bucky stepped closer, the bad feeling bothering his mind increasing intensity. “Y/N, this is a trap. Racing Pierce for this failsafe… it’s exactly what he wants. He’s counting on you going after it, to put yourself in danger.”

“I don’t have a choice, Bucky. If he gets to it first, it’s over. We need to find out exactly what this failsafe is and how to disable it.”

Bucky’s hand twitched, his instincts screaming at him that something was terribly wrong. 

“I don’t like this,” he admitted, his voice low and urgent. “There’s too much we don’t know. Pierce is desperate, and that makes him dangerous. If you go after this, you’re walking right into his hands.”

“Not if we get to it first.” You lifted your hand and placed it over his cheek, “Trust me.”

Not even ten minutes passed by, Lawson returned, his expression urgent. “Ma’am, we’ve got the preliminary intel on Project Lazarus. It’s worse than we thought.”

Your eyes snapped to Lawson, all traces of emotion vanishing as you switched back into mission mode. “What did you find?”

Lawson handed you a small, encrypted drive. “It’s a self-destruct mechanism, embedded within the serum dna. If activated, it will kill the host instantly. Pierce’s plan is to get to the activation device before we can, and if he does...”

Y/N’s grip on the drive tightened, the weight of what Lawson was saying settling over her. “Then it’s game over,” you finished, your voice cold.

Bucky felt his chest tighten as he listened. “Y/N, this is exactly what I was talking about. You’re the one he’s targeting. He knows you’ll go after it, and he’s setting the trap.”

You looked down at the drive in your hand, the realization of what you were up against hitting you fully. But there was no hesitation in your voice when you spoke. “This is why we make sure he doesn’t get there first. We move fast, and we don’t give him the chance to spring his trap.”

“Lawson, get the team ready, we’re moving out in twenty.” You look back at Bucky, giving him a small reassuring smile before you get yourself prepared.

× × × ×

The war room at the Avengers Compound was buzzing with activity. Multiple holographic displays floated in midair, showing maps, satellite images, and streams of data that scrolled rapidly across the screens. The Avengers were gathered around the central table, their expressions tense and focused. Time was running out, and they all knew it.

Steve Rogers stood at the head of the table, his arms crossed as he surveyed the data being fed in by JARVIS. His eyes were sharp, determined, but there was an undercurrent of urgency in his posture. They had to find Pierce before it was too late.

“JARVIS, expand the search radius to include the most recent HYDRA activity,” Tony Stark ordered, his fingers flying over the controls on the table. A new map appeared, showing several locations highlighted in red.

“I have already cross-referenced known HYDRA safehouses and recent communications intercepts, sir,” JARVIS responded, his voice calm and efficient. “However, Mr. Pierce has been taking measures to cover his tracks. He is not using any of the usual channels.”

“We’re missing something,” Natasha Romanoff said, her voice thoughtful as she studied the data in front of her. “He wouldn’t just go dark. He’s got to be coordinating something big. There has to be a pattern.”

Bruce Banner leaned forward, adjusting his glasses as he examined a cluster of highlighted areas on the map. “What about his known associates? Anyone still in contact with him?”

“Most of them are either in custody or underground,” Natasha replied, shaking her head. “But there are a few we haven’t been able to track down. They could be his lifeline.”

Steve looked over at Sam Wilson, who was monitoring a live feed of communications traffic. “Sam, anything on your end?”

“Not much,” Sam said, his brow furrowed in concentration. “But I did pick up a few encrypted messages that could be HYDRA-related. The problem is, they’re using a code we haven’t cracked yet.”

Tony’s fingers paused over the controls, and he turned to look at the team. “We don’t have time to play it safe. We need to flush him out. We hit those locations simultaneously and force him to react.”

“Agreed,” Steve said, nodding. “But we need to be smart about it. Pierce is desperate, and that makes him dangerous. We can’t afford any mistakes.”

“Steve’s right,” Natasha added. “We go in, but we need to be prepared for anything.”

Just then, JARVIS’s voice cut through the tension. “I may have something, sir. I’ve detected a series of anomalous energy readings in an isolated area approximately fifty miles outside the city. The readings are consistent with the signature of a stealth HYDRA base.”

A holographic image zoomed in on the location, showing a heavily forested area with minimal infrastructure. The perfect place for someone like Pierce to lay low.

“That’s got to be him,” Clint Barton said, his eyes narrowing as he studied the terrain. “It’s secluded, hard to access, and easy to defend.”

Steve nodded, his jaw tightening. “Then that’s where we’re going.”

Tony was already moving toward his suit, the familiar whirring of machinery filling the room as the armor began assembling around him. “JARVIS, prepare the Quinjet. We’re wheels up in five.”

The rest of the team sprang into action, suiting up and gathering their gear. The sense of urgency in the room was palpable—this was it. The moment they had been waiting for.

As they headed toward the hangar, Steve couldn’t shake the nagging feeling that something was off. They had the location, they had the team, but there was still an unknown element at play—something that Pierce was counting on. They had to be ready for whatever he had planned.

In the Quinjet, the team settled into their positions, the hum of the engines vibrating through the floor as they prepared for takeoff. Steve stood at the front, his hands gripping the back of the pilot’s seat as he stared out at the darkening sky.

“We go in fast and hard,” he said, addressing the team. “We neutralize any resistance and secure Pierce. But keep your eyes open—this could be a trap.”

“Isn’t it always?” Natasha remarked, a hint of wry humor in her voice as she checked her weapons.

Tony turned to face the team, his visor down, but his voice clear through the comms. “Remember, Pierce knows how we operate. We need to stay one step ahead.”

The Quinjet lifted off smoothly, banking toward the coordinates JARVIS had provided. As they flew toward what they hoped would be the final confrontation with Pierce, the tension in the air was almost suffocating. Each of them was lost in their own thoughts, preparing mentally for what was to come.

Steve’s mind, however, drifted to Y/N and Bucky. He knew they were out there too, likely chasing the same target. His grip tightened on the seat. He hoped they’d stay out of each other’s way, but deep down, he knew that wasn’t likely.

As the Quinjet sped through the night, Steve glanced around at his team, each one of them ready to face whatever lay ahead. This was the moment they’d been preparing for. There was no turning back now.

“Approaching the target location,” Sam announced from the cockpit, bringing Steve’s focus back to the mission. “We’re going in.”

Steve took a deep breath, steeling himself for the battle to come. “Let’s finish this.”

The Quinjet descended toward the forest below, and the Avengers braced themselves for the confrontation that would determine the fate of their mission—and possibly much more.

The air inside the abandoned HYDRA facility was thick with tension. Dust and debris lined the cold metal floors, remnants of a once formidable operation. Now, it was eerily silent, save for the faint hum of electricity still pulsing through the aging wires. The Avengers moved cautiously, their steps echoing through the empty corridors.

Steve Rogers led the team, his shield at the ready, as they approached the central chamber. His instincts told him they were close—too close for comfort. The door to the chamber slid open with a hiss, revealing a large, dimly lit room. And there, at the center, stood Bucky and you.

The Avengers froze, surprise flickering across their faces as they took in the sight of the two figures. Bucky’s stance was tense, defensive, while you stood beside him, your gaze throwing daggers.

“Well, well, well,” Tony Stark’s voice cut through the silence, dripping with sarcasm. “Look who decided to crash the party. You two got lost on your way to a HYDRA reunion?”

You smirked, crossing your arms as you eyed Tony. “Funny, Stark. I was going to say the same thing about you. Shouldn’t you be off with your lab toys or something? Or is this just another Tuesday for you?”

Tony raised an eyebrow, his suit whirring softly as he adjusted his stance. “Oh, you know me, always multitasking. But I gotta admit, seeing you two here is a bit of a plot twist. Care to explain what’s going on, or are we doing this the hard way?”

Bucky’s eyes darted between you and the Avengers, his muscles coiled like a spring ready to snap. He didn’t like how outnumbered you were, and he especially didn’t like the idea of a fight breaking out with Steve standing just a few feet away.

You kept your gaze locked on Tony, a hint of a smirk still playing on your lips. “Trust me, Stark, you don’t want to do this the hard way. We’re not here for you. But we’re also not here for a social call.”

Steve, sensing the rising tension, stepped forward, his shield raised slightly. “Y/N, Bucky, We’re here to take down Pierce. You’re either with us or against us.”

Your eyes flicked to Steve, your smirk fading as your expression hardened. “We’re here for the same reason, Rogers. But our priority isn’t taking down Pierce. It’s beating him first.”

Tony tilted his head, his eyes narrowing behind his mask. “And what, pray tell, what could be worse than letting that slimeball run free? Because from where I’m standing, it looks like you’re cozying up to the wrong side.”

Before you could respond, a slow clap echoed through the chamber, drawing everyone’s attention to the shadows. From the darkness, Pierce emerged, a smirk plastered on his face as he surveyed the scene.

“Well, isn’t this touching?” Pierce drawled, his voice dripping with condescension. “The Avengers, all united in their righteousness, and yet… here you are, just as powerless as ever.”

The Avengers immediately shifted their focus to Pierce, weapons ready, but the tension in the air remained palpable. Your body tensed, your eyes narrowing as you locked onto Pierce.

Steve took a step forward, his voice stern. “Pierce, it’s over. Surrender now, and maybe we can work something out.”

Pierce laughed, a cold, humorless sound that echoed through the chamber. “Oh, Steve. Always the optimist. But you’re wrong. It’s not over. In fact, it’s just beginning.”

He raised a small device in his hand, his thumb hovering over a button. “You all really thought you could stop me? After everything HYDRA has built? The world may see me as a traitor now, but I’m not the one who’s lost here. You are.” 

Pierce’s eyes shifted to you, his expression darkening with malicious glee. “And you, Y/N… the precious little weapon we created. Did you really think you could escape us? That you could fight back and win? You’re a tool, nothing more. And like any tool, when you’ve outlived your usefulness, you get discarded.”

Your jaw clenched, but you remained silent, your mind racing. You could see the cold resolve in Pierce’s eyes, the unmistakable sign that he was ready to press the button at any moment.

Pierce continued, enjoying every moment of his twisted power. “This—this is my failsafe. Our failsafe. HYDRA always plans for every eventuality, and you, Y/N, were no exception. One press of this button, and everything you are, everything we made you, ends.”

Steve’s heart sank as Pierce’s words sunk in. The realization hit him hard—Pierce wasn’t just threatening everyone, he was threatening your life. Panic flashed across Steve’s face, and he took another step forward, his voice urgent and desperate. “Pierce, listen to me. You don’t have to do this. Whatever HYDRA promised you, it’s not worth it. Just… put the remote down, and we can figure this out.”

Pierce’s smirk widened, relishing the fear he saw in Steve’s eyes. “Oh, Captain. You think you can negotiate with me? That’s adorable. But you see, I’ve already won. You’re too late to stop me.”

Steve shook his head, his voice breaking as he pleaded, “You don’t have to end her like this. If it’s me you want, take me. Just don’t hurt her. I’ll do whatever you want—just put the remote down.”

For a brief moment, Pierce seemed to consider Steve’s words, tilting his head as if weighing his options. Then, a cold, cruel smile twisted his lips. “You think I’d trade her life for yours? How noble of you, Captain. But no, this isn’t about you. This is about making sure she never defies us again. It’s about sending a message.”

Steve’s desperation turned to anger as he tried one last time. “Pierce, if you do this, there’s no coming back. You’ll lose everything. We’ll make sure of it. Just… don’t press that button. We can find another way—”

But Pierce’s thumb was already pressing down. 

To Steve, it felt like the world slowed to a crawl. The small, mechanical click of the button echoed in his ears like a gunshot, louder than anything he’d ever heard. His heart pounded, time moving painfully slow as the full reality of what was happening crushed down on him.

“No!” Steve’s voice ripped through the chamber, filled with anguish and fear. He lunged forward, but it was too late. The failsafe activated, and your body reacted instantly.

Your body jerked violently as if a surge of electricity had just shot through your veins. A choked gasp escaped your lips, your eyes widening in shock and pain. You tried to reach out, to grab onto something, anything, to steady yourself but your strength drained from you like water through a sieve. You staggered, your legs buckling beneath you as you collapsed to the ground. Your vision blurred, but you fought for as long as you could, looking at Bucky who assisted you to collapse gently on the floor.

“No!” Steve’s voice was frantic, the raw pain in it reverberating through the room. He dropped to his knees beside you, cradling your convulsing form in his arms as if by holding you close, he could somehow stop the inevitable. “No, no, no, please, Y/N! Stay with me, stay with me!”

But you, through the haze of pain and the encroaching darkness, knew what you had to do. You turned your head slightly, focusing on Bucky. Your voice was weak, but the urgency was unmistakable. “Bucky… run. You have to… run. Don’t… look back.”

Bucky’s eyes widened in confusion, refusal written all over his face. “What? No, I’m not leaving you—”

“Go!” Your voice cracked with desperation as you struggled to maintain consciousness. “Go, Bucky! Now!”

Bucky hesitated for a fraction of a second, torn between his instinct to fight and his need to protect you. But then Pierce’s smug smile caught his eye, and something inside him snapped. With a roar of pure rage, Bucky’s metal arm slammed into Pierce, sending him crashing to the ground.

The fight was brutal, savage. Bucky’s fury was unleashed in every punch, every strike. Pierce barely had time to react before Bucky was on him, his fists a blur of metal and flesh, pummeling the life out of him.

Pierce’s screams echoed through the chamber, but Bucky didn’t stop. He didn’t stop until Pierce was nothing but a lifeless, broken mess on the floor. Panting, his vision clouded with rage and grief, Bucky finally looked up, his eyes locking onto your still form, cradled in Steve’s arms. The sight tore at his soul, but your final command echoed in his mind.

Run.

Without another word, Bucky turned and ran, disappearing into the shadows before the Avengers could even think to stop him.

Steve didn’t notice Bucky’s departure. His entire world had narrowed to you—your lifeless body, the cold stillness of your form in his arms. He rocked back and forth slightly, his grip tightening as if he could hold onto your life, refusing to let it slip away. His chest heaved with ragged breaths, his mind reeling with disbelief and sorrow.

The other Avengers stood around him, silent and stunned, the gravity of what had just happened sinking in. They had all faced countless dangers before, but this felt different. 

This felt final.

Steve’s voice broke the silence, a guttural cry of anguish that echoed through the chamber, filled with a depth of sorrow that none of them had ever heard from him before. 

“Y/N!” he cried out, his voice trembling, pleading with a world that seemed to have gone cold and unfeeling. “Please, don’t leave me. You can’t… no…no…please.”

He cradled you closer, his tears falling unchecked, soaking into your hair. Every fiber of his being screamed against what was happening. This wasn’t supposed to be how it ended. Not for her, not like this. The injustice of it tore at him, threatening to pull him apart.

But you didn’t respond. Your body remained still, your face peaceful, as if you had simply gone to sleep. The life that had burned so brightly within you was gone, extinguished by the cruel machinations of those who had sought to control you. He couldn’t stop the sob that tore from his throat, his anguish laid bare for all to see. 

“I’m sorry… I’m so sorry…” he whispered, his voice breaking with every word. 

The chamber seemed to grow colder, the weight of the moment pressing down on everyone. Time seemed to stand still, the world outside forgotten, as they all stood in the aftermath of a battle that had been lost in the most devastating way possible.

After what felt like an eternity, Tony finally stepped forward, his voice quiet, almost reverent. “Steve…” he began, but the words caught in his throat. What could he possibly say? There was no comfort, no way to undo what had been done.

Steve didn’t respond. His focus was entirely on you, his hand gently brushing a strand of hair away from your face. He couldn’t comprehend a world without you in it. He didn’t want to.

Finally, after a long, painful silence, Natasha spoke, her voice soft but firm. “We need to get her out of here, Steve.”

Steve nodded numbly, but he didn’t move. It took all of his strength to gather himself enough to even consider letting you go. He didn’t want to let you go. The idea of it was unbearable. But he knew Natasha was right. They couldn’t leave you here, not in this place, not like this.

With a deep, shuddering breath, Steve slowly stood, cradling you in his arms as if you were the most precious thing in the world—because to him, you were. He turned toward the exit, his steps heavy, each one feeling like it might be his last.

Steve couldn’t help but glance back one last time, as if hoping to see some sign that this wasn’t real—that you would wake up, that this nightmare would end. But there was nothing. Just the emptiness of a place that had taken so much from them.

They stepped out into the daylight, the sun glaringly bright against the backdrop of their sorrow. Steve’s grip on you tightened, and he pressed a soft kiss to your forehead, his heart breaking all over again. 

“I love you,” he whispered, the words catching in his throat. “I always will.”

But there was no answer, no sign of life. You were gone, and the world felt infinitely colder because of it.

So they left, carrying with him a loss that would never fully heal, a wound that would forever mark him. Steve walked with his head bowed, his shoulders slumped, the light in his eyes dimmed by the grief that had taken hold of him. 

[tomorrowusa]

The MAGA-DOGE régime decided that the middle of hepatitis outbreak was a great time to close the CDC's Division of Viral Hepatitis.

After people started testing positive for hepatitis C in a coastal Florida town in December, state officials collected blood from patients, wrapped their specimens in dry ice and mailed them straight to the Centers for Disease Control in Atlanta, Ga. The hepatitis C virus, which is spread through contact with infected blood and can lead to deadly liver cancer, is notoriously hard to identify. But if anyone could understand what was happening in Florida, it would be the Division of Viral Hepatitis in the CDC's headquarters. Using samples from the laboratory's collection of nearly 1 million frozen specimens, scientists helped make the initial discovery of the hepatitis C virus in the 1980s. In 2020, that research was awarded the Nobel Prize in Medicine.

Unlike Trump, the scientists at the lab knew what they were doing.

The scientists at the lab knew what they were doing. Quickly, they analyzed the blood from Florida using their custom software and found that nine cases were genetically linked to the same pain clinic, where it was later discovered that a doctor was improperly reusing injection vials. By March, officials in Florida had restricted the doctor's medical license to limit the spread of the virus and packaged new patient samples to send to the CDC for testing, CDC employees told NPR. But on April 1, the outbreak investigation was brought to a halt. All 27 of the lab's scientists received an email from the U.S. Department of Health and Human Services informing them that they were losing their jobs. Like thousands of other employees who received similar emails that day, the scientists were told they would be placed on administrative leave until June 2, after which they would no longer work for the CDC. The email said their duties were "identified as either unnecessary or virtually identical to duties being performed elsewhere in the agency." But the kind of genetic tracing that the CDC's lab performs is not conducted by any other lab in the United States or the world, experts interviewed by NPR said. While the lab remains shuttered, ongoing investigations of current hepatitis outbreaks have been stalled, not just in Florida, but also in Oregon, Pennsylvania, Massachusetts, New Mexico, Wisconsin, West Virginia and Georgia, according to CDC employees who work closely with the Division of Viral Hepatitis. The five CDC employees NPR spoke with requested that their names not be shared for fear of retaliation.

For all anybody knows, the scientists may have been fired by Apartheid Elon's teen buddy Big Balls.

The Trump administration is making people sick and keeping them sick. And this is just the start.

[reasonsforhope]

"When bloodstream infections set in, fast treatment is crucial — but it can take several days to identify the bacteria responsible. A new, rapid-diagnosis sepsis test could cut down on the wait, reducing testing time from as much as a few days to about 13 hours by cutting out a lengthy blood culturing step, researchers report July 24 [2024] in Nature.

“They are pushing the limits of rapid diagnostics for bloodstream infections,” says Pak Kin Wong, a biomedical engineer at Penn State who was not involved in the research. “They are driving toward a direction that will dramatically improve the clinical management of bloodstream infections and sepsis.”

Sepsis — an immune system overreaction to an infection — is a life-threatening condition that strikes nearly 2 million people per year in the United States, killing more than 250,000 (SN: 5/18/08). The condition can also progress to septic shock, a steep drop in blood pressure that damages the kidneys, lungs, liver and other organs. It can be caused by a broad range of different bacteria, making species identification key for personalized treatment of each patient.

In conventional sepsis testing, the blood collected from the patient must first go through a daylong blood culturing step to grow more bacteria for detection. The sample then goes through a second culture for purification before undergoing testing to find the best treatment. During the two to three days required for testing, patients are placed on broad-spectrum antibiotics — a blunt tool designed to stave off a mystery infection that’s better treated by targeted antibiotics after figuring out the specific bacteria causing the infection.

Nanoengineer Tae Hyun Kim and colleagues found a way around the initial 24-hour blood culture.

The workaround starts by injecting a blood sample with nanoparticles decorated with a peptide designed to bind to a wide range of blood-borne pathogens. Magnets then pull out the nanoparticles, and the bound pathogens come with them. Those bacteria are sent directly to the pure culture. Thanks to this binding and sorting process, the bacteria can grow faster without extraneous components in the sample, like blood cells and the previously given broad-spectrum antibiotics, says Kim, of Seoul National University in South Korea.

Cutting out the initial blood culturing step also relies on a new imaging algorithm, Kim says. To test bacteria’s susceptibility to antibiotics, both are placed in the same environment, and scientists observe if and how the antibiotics stunt the bacteria’s growth or kill them. The team’s image detection algorithm can detect subtler changes than the human eye can. So it can identify the species and antibiotic susceptibility with far fewer bacteria cells than the conventional method, thereby reducing the need for long culture times to produce larger colonies.

Though the new method shows promise, Wong says, any new test carries a risk of false negatives, missing bacteria that are actually present in the bloodstream. That in turn can lead to not treating an active infection, and “undertreatment of bloodstream infection can be fatal,” he says. “While the classical blood culture technique is extremely slow, it is very effective in avoiding false negatives.”

Following their laboratory-based experiments, Kim and colleagues tested their new method clinically, running it in parallel with conventional sepsis testing on 190 hospital patients with suspected infections. The testing obtained a 100 percent match on correct bacterial species identification, the team reports. Though more clinical tests are needed, these accuracy results are encouraging so far, Kim says.

The team is continuing to refine their design in hopes of developing a fully automated sepsis blood test that can quickly produce results, even when hospital laboratories are closed overnight. “We really wanted to commercialize this and really make it happen so that we could make impacts to the patients,” Kim says."

-via Science News, July 24, 2024