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[newcellfend]

[kasradoc]

‏‎. افزودنی های غذایی : در سال های اخیر، همه مردم در جهان « توسعه یافته » بطور فزاینده ای نسبت به مسائل سلامتی مربوط به مواد غذایی آگاه شده اند. این امر تا حدودی به خاطر دانش افزوده ایجاد شده توسط تحقیقات کلینیکی و تا اندازه ای بخاطر انتشار این اطلاعات و اعمال نفوذ سازماندهی شده توسط گروه های محیطی و سود ویژه می باشد. بعنوان مثال از ایزو در حال حاضر به طور گسترده ای مشاهده شده است که عادات غذایی می تواند نقش مهمی در پیشگیری از امراضی همچون فشار خون بالا، هیپرلپیدی و سرطان روده ایفا کنند. در پاسخ، صنعت مواد غذایی گروهی از مواد غذایی را با عنوان مواد غذایی کاربردی یا nutra ceuticals ارائه داده است. در این طبقه بندی برخی از مواد غذایی فقط با استفاده درست از ترکیبات غذایی همچون کاهش چربی در فرآورده های کم چرب یا افزایش اشباع نشده های متعدد در نظر گرفته می شوند. در حالیکه سایرین ممکن است جهت ایفا نقش مثبت تری مورد ملاحظه قرار گیرند. افزودنی های غذایی بکار رفته در این گروه بعنوان موادی بدون ارزش غذایی تعریف شده اند. اما آنها جهت حفظ سلامتی و یا عمل بعنوان یک عنصر درمانی کاربرد دارند. مواد غذایی کاربردی شرح داده شده فوق با نیازهای غذایی همه مردم در نظر گرفته می شوند. بالعکس، اقلیت هایی که به مواد غذایی ساخته شده خاص برای شرایط غذایی پزشکی ویژه آنها می توانند شناسایی شوند. دانلود نمایید: https://www.kasradoc.com/ #powerpoint #Document #Kasradoc #ppt #پاورپوینت #مقاله #پروژه #کسری_داک #افزودنیهای_غذایی #افزودنیهای_غیر_مجاز_در_مواد_غذایی #افزودنیهای_مجاز_خوراکی #افزودنیهای_مواد_غذایی_مقاله #پلان_کارخانه_مواد_غذایی #دنیای_افزودنیهای_مواد_غذایی #منظور_از_افزودنیهای_غذایی_چیست #کیفیت #رنگ #طعم #مزه #افزایش_ماندگاری_غذا #اسیدی_کننده_ها #تنظیم_کننده_اسیدیته #عوامل_ضد_کلوخه‌_ای #ضد_کف #آنتی‌_اکسیدان‌_ها #عوامل_پرکننده #عوامل_نگهداری_رنگ #امولسیفایرها #طعم_دهنده_ها #نگهدارنده_ها‎‏ (در ‏‎Kasradoc‎‏) https://www.instagram.com/p/CSyYOdZI1qA/?utm_medium=tumblr

[nhene56]

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Biofield Energy Healing Based Vitamin D3: An Improved Overall Bone Health Activity in MG-63 Cell Line

Abstract

The potential of The Trivedi Effect®-Consciousness Energy Healing vitamin D3 and DMEM medium was investigated in human bone osteosarcoma cells (MG-63). The Test Items (TI) i.e. vitamin D3 and DMEM were separated into two parts. Bone health parameters were tested such as Alkaline Phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Karen Byrnes Allen and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the Untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability more than 71% with safe and nontoxic profile among test samples on MG-63 cell line. The level of ALP was significantly increased by116.4% at 50μmL in the UT-DMEM+BT-TI group, while 35.6%, 81.2%, and 47.9% at 10, 50 and 100 μmL, respectively in the BT-DMEM+UT-TI group. In addition, ALP level was increased by 42.3% and 90.6% at 10 and 50 μ mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated group. The level of collagen was significantly increased by 10.5% and 110.1% at 0.1 and 1 μmL, respectively in the UT-DMEM+BT-TI group, while 22.6% and 190.6% at 0.1 and 1μmL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 140.6% and 39.9% at 0.1 and 1 μmL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 128.7% at 50 μmL, in the UT-DMEM+BT-TI group, while 34.1%, 154.7%, and 18.7% at 1, 10, and 50 μmL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 105.7%, 78.1%, and 39.6% at 1, 10, and 50 μmL, respectively as compared with the untreated group. Thus, the study results concluded that The Trivedi Effect® Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone disorders along with wide range of adverse bone health conditions

Keywords:  The Trivedi Effect®; Bone Health; Biofield Healing; Osteosarcoma Cells; Vitamin D; Bone mineralization

Abbreviations:  CAM: Complementary and Alternative Medicine, NCCAM: National Center for Complementary and Alternative Medicine; MG- 63: Human Bone Osteosarcoma Cells, ALP: Alkaline phosphatase, DMEM: Dulbecco's Modified Eagle's Medium; FBS: Fetal bovine serum; EDTA: Ethylene Diamine Tetra Acetic Acid, UT: Untreated, BT: Biofield Energy Treated, TI: Test Item.

    Introduction

Vitamin D has multiple effects which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems. Moreover, it has sig-nificant anti-inflammatory, anti-arthritic, anti-osteoporosis, anti-stress, anti-aging, anti-apoptotic, wound healing, anti-cancer, anti-psychotic, and anti-fibrotic roles. Vitamin D receptors (VDRs) are widely present in most of the body organs like brain, heart, lungs, kidney, liver, pancreas, large and small intestines, muscles, reproductive, nervous system, etc. [1]. VDRs influence cell-to- cell communication, normal cell growth, cell differentiation, cell cycling and proliferation, hormonal balance, neurotransmission, skin health, immune and cardiovascular functions. Bone-related health issues become a major problem among the population from village to the cities. Vitamin D plays a vital role in preserving a healthy mineralized skeleton of most of the vertebrates including humans. Cod liver oil, irradiation of other foods including plants, sunlight, etc. are found to be effective against bone related disorders, which lead to discovering the active principle- vitamin D [1]. The role of vitamin D has been well defined not only for improving the bone mineralization but also with increased bone resorption, aging, inflammation and overall quality of life. Vitamin D3 is synthesized in the skin by sunlight and once formed it sequentially metabolized in the liver and kidney to 1,25-dihydroxyvitamin D (calcitriol, the vitamin D hormone) [2]. Calcitriol play an important role in maintaining the normal level of calcium and phospho-rus, promotes bone mineralization, induce or repress the genes responsible for conserving the mineral homeostasis and skeletal integrity, and inhibit hypertension, kidney damage, cardiovascular and immune disorders (such as Lupus, Addison Disease, Graves' Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Anemia, Sjogren Syndrome, Systemic Lupus Erythemato-sus, Diabetes, Alopecia Areata, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis), and the secondary hyperparathyroidism [3]. Vitamin D insufficiency and deficiency is the major health problem, which causes metabolic bone disease in the young and elderly populations [4]. Fortified foods have a variable amount of vitamin D and most of the foods do not contain vitamin D, which can be fulfilled using some supplements. In order to avoid the bone related disorders such as osteomalacia, exacerbate osteoporosis, hyperparathyroidism, immune disorders, etc. calcium 1000-1500mg/day along with vitamin D supplement around 400 IU/day is very important for maintaining the good bone health [5].

Various in vitro studies have readily established the role of bone health using cell lines and its resorbing effects using three important key biomarkers, such as alkaline phosphatase (ALP), collagen and calcium. MG-63 cell line derived from juxta cortical osteosarcoma, which represents an immature osteoblast phenotype and undergoes temporal development in long term culture. The response of MG-63 cells to 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) administration has been studied to be similar to normal human osteoblast cells [6]. Hence, MG-63 cell line is widely used for studying the potential of any test compounds to improve the bone health [7]. The formation of new bone involves a complex series of events including the proliferation and differentiation of osteoblasts, and eventually the formation of a mineralized extracellular matrix. ALP is a phenotypic marker for the early differentiation and maturation of osteoblasts. ALP increases the local concentration of inorganic phosphate for bone mineralization and hence is an important marker for osteogenic activity [8]. Similarly, active osteoblasts synthesize and extrude collagen, which plays an important role in the formation of bone extracellular matrix by providing strength and flexibility. Collagen fibrils formed an arrays of an organic matrix known as Osteoid [9]. Likewise, calcium phosphate is deposited in the Osteoid and gets mineralized (combination of calcium phosphate and hydroxyapatite) and provides rigidity to the bone [10]. Thus, these parameters are very essential in order to study the bone health in cell lines. Authors evaluated the in vitro effect of the Biofield Energy Treated vitamin D3 as a test item, a Complementary and Alternative Medicine (CAM) on bone health using MG-63 cell line for major biomarkers.

Within the burgeoning ground of CAM therapies, Biofield En-ergy Treatment or energy medicine, is emerging with significant benefits in various scientific fields. The effects of the CAM ther-apies have great potential, which include external qigong, Johrei, Reiki, therapeutic touch, yoga, Qi Gong, polarity therapy, Tai Chi, pranic healing, deep breathing, chiropractic/osteopathic manipu-lation, guided imagery, meditation, massage, homeopathy, hypno-therapy, progressive relaxation, acupressure, acupuncture, special diets, relaxation techniques, Rolfing structural integration, healing touch, movement therapy, pilates, mindfulness, Ayurvedic medi-cine, traditional Chinese herbs and medicines in biological systems both in vitro and in vivo[11]. Biofield Energy Healing Treatment (The Trivedi Effect®) contain a putative bioenergy, which is channeled by a renowned practitioners from a distance. Biofield Energy Healing as a CAM showed a significant results in biological studies [12]. However, the National Center for Complementary and Alternative Medicine (NCCAM), well-defined Biofield therapies in the subcategory of Energy Therapies [13]. The Trivedi Effect®- Consciousness Energy Healing Treatment has been reported with significant revolution in the physicochemical properties of metals, chemicals, ceramics and polymers [14-16], improved agricultural crop yield, productivity, and quality [17,18], transformed antimicrobial characteristics [19,20], bone health [21,22], biotechnology [23], improved bioavailability [24-26], skin health [27,28], nutra- ceuticals [29,30], cancer research [31,32], and human health and wellness.

Based on the significant outcomes of Biofield Energy Treatment and vital role of vitamin D3 on bone health, authors sought to evaluate the impact of the Biofield Energy Treatment (The Trivedi Effect®) on vitamin D3 as test sample for bone health activity with respect to the assessment of different bone health parameters like ALP, collagen content, and bone mineralization using standard in vitro assays in MG-63 cells.

    Material and Methods

Chemicals and reagents

Rutin hydrate was purchased from TCI, Japan, while vitamin D3 (denoted as test item) and L-ascorbic acid were obtained from Sigma-Aldrich, USA. Fetal Bovine Serum (FBS) and Dulbec- co's Modified Eagle's Medium (DMEM) were purchased from Life Technology, USA. Antibiotics solution (penicillin-streptomycin) was procured from HiMedia, India, while 3-(4, 5-diamethyl-2-thi- azolyl)-2, 5-diphenyl-2H-tetrazolium) (MTT), Direct Red 80, and ethylene diamine tetra acetic acid (EDTA) were purchased from Sigma, USA. All the other chemicals used in this experiment were analytical grade procured from India.

Cell culture

Human bone osteosarcoma cell line -MG-63 was used as test system in the present study. The MG-63 cell line was maintained in DMEM growth medium for routine culture supplemented with 10% FBS. Growth conditions were maintained as 37 °C, 5% CO2 and 95% humidity and sub cultured by trypsinisation followed by splitting the cell suspension into fresh flasks and supplementing with fresh cell growth medium. Three days before the start of the experiment, the growth medium of near-confluent cells was replaced with fresh phenol-free DMEM, supplemented with 10% charcoal dextran stripped FBS (CD-FBS) and 1% penicillin-strep-tomycin [33].

Experimental design

The experimental groups consisted of cells in baseline control, vehicle control groups (0.05% DMSO with Biofield Energy Treated and untreated DMEM), positive control group (rutin hydrate) and experimental test groups. The experimental groups included the combination of the Biofield Energy Treated and untreated vitamin D3/DMEM. It consisted of four major treatment groups on specified cells with Untreated-DMEM + Untreated-Test item (UT-TI), UT-DMEM + Biofield Energy Treated test item (BT-TI), BT-DMEM + UT-TI, and BT-DMEM + BT-TI.

Consciousness energy healing treatment strategies

The test item and DMEM were divided into two parts. One part each of the test item and DMEM was treated with the Biofield Energy by a renowned Biofield Energy Healer (also known as The Trivedi Effect®) and coded as the Biofield Energy Treated item, while the second part did not receive any sort of treatment. This Biofield Energy Healing Treatment was provided by Karen Byrnes Allen remotely for ~5 minutes. Biofield Energy Healer was remotely located in the USA, while the test samples were located in the research laboratory of Dabur Research Foundation, New Delhi, India. This Biofield Energy Treatment was administered for 5 minutes through the Healer's unique Energy Transmission process remotely to the test samples under laboratory conditions. Karen Byrnes Allen in this study never visited the laboratory in person, nor had any contact with the test item and medium. Further, the control group was treated with a sham healer for comparative purposes. The sham healer did not have any knowledge about the Biofield Energy Treatment. After that, the Biofield Energy Treated and untreated samples were kept in similar sealed conditions for experimental study.

Determination of non-cytotoxic concentration

The cell viability was performed by MTT assay in human bone osteosarcoma cell line (MG-63). The cells were counted and plated in 96 well plates at the density corresponding to 5 X 103 to 10 X 103 cells/well/180 μL of cell growth medium. The above cells were incubated overnight under growth conditions and allowed the cell recovery and exponential growth, which were subjected to serum stripping or starvation. The cells were treated with the test item, DMEM, and positive control. The untreated cells were served as baseline control. The cells in the above plate(s) were incubated for a time point ranging from 24 to 72 hours in CO2 incubator at 37 °C, 5% CO2, and 95% humidity. Following incubation, the plates were taken out and 20μL of 5mg/mL of MTT solution were added to all the wells followed by additional incubation for 3 hours at 37 °C. The supernatant was aspirated and 150μL of DMSO was added to each well to dissolve formazan crystals. The absorbance of each well was read at 540 nm using Synergy HT micro plate reader, BioTek, USA [34]. The percentage cytotoxicity at each tested concentrations of the test substance were calculated using the

following equation (1):

% Cytotoxicity = (1 - X / R) *100 (1)

Where, X = Absorbance of treated cells; R = Absorbance of untreated cells

The percentage cell viability corresponding to each treatment was obtained using the following equation (2):

% Cell Viability = 100 - % Cytotoxicity (2)

The concentrations exhibiting >70% Cell viability was considered as non-cytotoxic.

Assessment of alkaline phosphatase (ALP) activity

The cells were counted using an hemocytometer and plated in a 24-well plate at the density corresponding 1 x 104 cells/well in phenol free DMEM supplemented with 10% CD-FBS. Following respective treatments, the cells in the above plate were incubated for 48 hours in CO2 incubator at 37 °C, 5% CO2, and 95% humidity. After 48hours of incubation, the plate was taken out and processed for the measurement of ALP enzyme activity. The cells were washed with 1X PBS and lysed by freeze thaw method i.e, incubation at -80 °C for 20 minutes followed by incubation at 37 °C for 10 minutes. To the lysed cells, 50μL of substrate solution i.e, 5mM of p-nitrophenyl phosphate (pNPP) in 1M diethanolamine and 0.24 mM magnesium chloride (MgCl2) solution (pH 10.4) was added to all the wells followed by incubation for 1 hour at 37 °C. The absorbance of the above solution was read at 405 nm using Synergy HT micro plate reader (Biotek, USA). The absorbance val-ues obtained were normalized with substrate blank (pNPP solution alone) absorbance values [33]. The percentage increase in ALP enzyme activity with respect to the untreated cells (baseline group) was calculated using equation (3):

% Increase =[(X -R) /R)]*100 (3)

Where, X = Absorbance of cells corresponding to positive control and test groups

R = Absorbance of cells corresponding to baseline group (untreated cells).

Assessment of collagen synthesis

The MG-63 cells were counted using a hemocytometer and plated in 24-well plate at the density corresponding to 10 x 103 cells/well in phenol free DMEM supplemented with 10% CD-FBS. Following respective treatments, the cells in the above plate were incubated for 48 hours in CO2 incubator at 37°C, 5% CO2, and 95% humidity. After 48 hours of incubation, the plate was taken out and the amount of collagen accumulated in MG-63 cells corresponding to each treatment was measured by Direct Sirius red dye binding assay. In brief, the cell layers were washed with PBS and fixed in Bouin's solution (5% acetic acid, 9% formaldehyde and 0.9% picric acid) for 1 hours at room temperature (RT). After 1 hour of incubation, the above wells were washed with milliQ water and air dried. The cells were then stained with Sirius red dye solution for 1 hour at RT followed by washing in 0.01 N HCl to remove un-bound dye. The collagen dye complex obtained in the above step was dissolved in 0.1 N NaOH and absorbance was read at 540 nm using Biotek Synergy HT micro plate reader. The level of collagen was extrapolated using standard curve obtained from purified Calf Collagen Bornstein and Traub Type I (Sigma Type III) [33]. The percentage increase in collagen level with respect to the untreated cells (baseline group) was calculated using equation (4):

% Increase = [(X - R) / R] *100 (4)

Where, X = Collagen levels in cells corresponding to positive control and test groups

R = Collagen levels in cells corresponding to baseline group (untreated cells).

Assessment of bone mineralization by alizarin red S staining

The MG-63 cells were counted using an hemocytometer and plated in 24-well plate at the density corresponding to 10 x103cells/well in phenol free DMEM supplemented with 10% CD- FBS. Following respective treatments, the cells in the above plate were incubated for 48 hours in CO2 incubator at 37°C, 5% CO2, and 95% humidity to allow cell recovery and exponential growth. Following overnight incubation, the above cells will be subjected to serum stripping for 24 hours. The cells will be then be treated with non-cytotoxic concentrations of the test samples and positive control. After 3-7days of incubation with the test samples and positive control, the plates were taken out cell layers and processed further for staining with Alizarin Red S dye. The cells were fixed in 70% ethanol for 1 hour, after which Alizarin Red solution (40 μm; pH 4.2) was added to the samples for 20 minutes with shaking. The cells were washed with distilled water to remove unbound dye. For quantitative analysis by absorbance evaluation, nodules were solubilized with 10% cetylpyridinium chloride for 15 minutes with shaking. Absorbance was measured at 562 nm using Biotek Synergy HT micro plate reader [33]. The percentage increase in bone mineralization with respect to the untreated cells (baseline group) was calculated using the following equation (5):

% Increase = [(X - R) / R] *100 (5)

Where, X = Absorbance in cells corresponding to positive control or test groups; R = Absorbance in cells corresponding to baseline (untreated) group.

Statistical analysis

All the values were represented as percentage of respective parameters. For multiple group comparison, one-way analysis of variance (ANOVA) was used followed by post-hoc analysis by Dun- netts test. Statistically significant values were set at the level of p≤0.05.

    Results and Discussion

Cell viability using MTT assay

Cell viability data showed that cell viability was significant im-proved percentage among the Biofield Energy Treated vitamin D3 and DMEM in MG-63 cells are shown in Figure 1. The data showed that the test samples found as nontoxic and safe (as evidence of cell viability approximately more than 71%) across all the tested concentrations up to 100μg/mL. Hence, the same concentrations were used for the evaluation of other bone health parameters such as alkaline phosphatase (ALP) activity, collagen synthesis, and bone mineralization in MG-63 cells.

Alkaline phosphatase (ALP) enzyme activity

The Trivedi Effect®-Energy of Consciousness Healing based test items displayed an increased ALP level in various groups (Figure 2) as compared with the untreated group. The percentage change in ALP data at various concentrations in different groups were presented in Figure 2 in terms of percentage values. The positive control, rutin showed a significant increased value by 43.44%, 53.55%, and 83.33% at 0.01, 0.1, and 1 μg/mL, respectively with respect to the untreated cells. The experimental test group's  untreated medium and Biofield Treated Test item (UT-DMEM+BT- TI) showed a significant increased level of ALP by 116.4% and 2.3% at 50 and 100 μg/mL, respectively while Biofield Treated medium and untreated Test item (BT-DMEM+UT-TI) showed a significant increased ALP level by 35.6%, 81.2%, and 47.9% at 10, 50 and 100 μg/mL, respectively as compared with the untreated test item and DMEM group. However, the Biofield Energy Treated medium and Biofield Energy Treated Test item (BT-DMEM+BT-TI) showed a significant increased ALP level by 42.3% and 90.6% at 10 and 50 μg/mL, respectively as compared with the untreated test item and DMEM group. ALP (zinc metalloprotein enzymes) is considered as one of the important bone biomarker proteins for osteoblast differentiation. Reduced level of bone ALP leads to various disorders such as obstructive liver disease, osteoblastic activity, metabolic bone disease, reduced bone growth, acromegaly, osteogenic sarcoma, or bone metastases, healing fracture, myelo-fibrosis, leukemia, and rarely myeloma [35-37]. Thus, the overall data suggested a significant improved ALP level after Biofield Energy Treatment. Biofield Energy Treated test samples could be useful against patients suffering from bone disorders to improve the skeletal structure and overall bone-related disorders.

Estimation of collagen synthesis

The Trivedi Effect®-Biofield Energy Treated test samples showed a significant enhanced level of collagen synthesis as compared with the untreated test samples. All the results are presented in percentage collagen values in Figure 3. The rutin hydrate (positive control group) showed a significant increased value of collagen by 46.59%, 51.97%, and 65.41% at 0.01, 0.1, and 1 μg/ mL, respectively. In addition, the experimental test groups such as UT-DMEM+BT-TI showed a significant increased collagen level by 10.5% and 110.1% at 0.1 and 1 μg/mL, respectively while BT- DMEM+UT-TI group showed a significant increased collagen level by 22.6%, 190.6%, and 1.6% at 0.1, 1, and 10 μg/mL, respectively as compared with the untreated test item and DMEM group. On the other hand, BT-DMEM+BT-TI group showed a significant increased collagen level by 140.6% and 39.9% at 0.1 and 1 μg/mL, respectively as compared with the untreated test item and DMEM group. Collagen synthesis leads to build stronger bone and muscles, which provide strength to skeletal structure. Collagen fibers provide strong mechanical force and strength to life. The most abundant matrix protein, collagen type I play a significant role in overall bone health [38,39]. Overall, the Consciousness Energy treated vitamin D3 had significantly improved the synthesis of collagen fibers in the human osteosarcoma cells with respect to all the treatment groups. Hence, it is assumed that The Trivedi Effect® has the significant potential to improve the bone health in various skeletal disorders against weaken joints, tendons, and ligaments.

Bone mineralization

The effect of Biofield Energy Treatment (Trivedi Effect®-Bio- field Energy Healing) on bone mineralization in MG-63 cells is shown in Figure 4, which showed a significant increased bone mineralization percentage in the test samples. Supplementation with calcium and vitamin D3 increased the chance of degree of bone mineralization. As, bioactive vitamin D or calcitriol is a steroid hormone, which has an important long term role in regulating the body levels of calcium and phosphorus, and in bone mineralization process. However, lack of vitamin D and calcium is directly linked to an inability of any person to fight against infections effectively, depression, muscle weakness, multiple sclerosis, fatigue and the development of diabetes, bone cancers, heart disease, high blood pressure, and stroke [40,41]. The results in term of percentage bone mineralization was presented in Figure 4. The positive control, rutin group showed a significant increased value of bone mineralization by 47.98%, 59.73%, and 139.02% at 5, 10, and 25 μg/mL, respectively. The experimental data among test group's   UT-DMEM+BT-TI showed a significant increased bone mineralization by 128.7% at 50 μg/mL, while BT-DMEM+UT- TI group showed a significantly increased bone mineralization by 34.1%, 154.7%, and 18.7% at 1, 10, and 50 μg/mL, respectively as compared with the untreated test item and DMEM group. However, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 105.7%, 78.1%, and 39.6% at 1, 10, and 50 μg/ mL, respectively as compared with the untreated test item and DMEM group. Thus, The Trivedi Effect®-Biofield Energy Treated vit D3 could be significantly useful to maintain a healthy skeletal structure for the patients suffering from various bone-related disorders.

    Conclusion

The experimental results of cell viability data using MTT assay showed more than 71% cells were viable and Consciousness Energy Healing based vitamin D3 further improved the cell viability. Thus, MTT data indicated that the test samples were safe and nontoxic in all the tested concentrations. However, various bone health parameters were significantly improved such as ALP was increased by 116.4% at 50 μg/mL in the UT-DMEM+BT-TI, while 35.6%, 81.2%, and 47.9% at 10, 50 and 100 μg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. In addition, BT-DMEM+BT-TI group showed an increased ALP level by 42.3% and 90.6% at 10 and 50 μg/mL, respectively The level of collagen was significantly increased by 10.5% and 110.1% at 0.1 and 1 μg/mL, respectively in the UT-DMEM+BT-TI, while 22.6%, 190.6%, and 1.6% at 0.1, 1, and 10 μg/mL, respectively in the BT-DMEM+UT-TI group. The level of collagen was increased by 140.6% and 39.9% at 0.1 and 1μg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone mineralization percent was significantly increased by 128.7% at 550 μg/mL in the UT-DMEM+BT-TI group, while 34.1%, 154.7%, and 18.7% at 1, 10, and 50 μg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT- TI group showed a significant increased bone mineralization by 105.7%, 78.1%, and 39.6% at 1, 10, and 50 μg/mL, respectively as compared with the untreated group. The Bone health parameters were significantly improved among the Biofield Energy Treated vitamin D3 test samples in MG-63 cells. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters  collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders osteoporosis, Paget's disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3can be useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in hormonal imbalance, aging, and various immune related disease conditions such as Multiple Sclerosis, Alzheimer's Disease, Asthma, Atherosclerosis, Pernicious Anemia, Aplastic Anemia, Diverticulitis, Graves' Disease, Dermatomyositis, Dermatitis, stress, Irritable Bowel Syndrome, Systemic Lupus Ery-thematosus, Hepatitis, Hashimoto Thyroiditis,Diabetes, Myasthe-nia Gravis, Ulcerative Colitis, Sjogren Syndrome, Parkinson's Dis-ease, etc. with a safe therapeutic index to improve overall health, and quality of life.

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Few Things About Pharmaceutical Granulation

Pharmaceutical granulation is a crucial process in the production of pharmaceuticals, where small particles are aggregated into larger granules to improve flowability, compressibility, and dosage uniformity. Here are a few key points about pharmaceutical granulation:

Purpose: Granulation helps in the formulation of solid dosage forms such as tablets and capsules. It enhances the flow properties of the powder mixture, reduces dustiness, improves compaction properties, and ensures uniform distribution of active pharmaceutical ingredients (APIs) and excipients.

Types of Granulation:

Wet Granulation: Involves the addition of a liquid binder to the powder mixture, followed by mixing, wet massing, drying, and sizing.

Dry Granulation: Also known as roller compaction, this method involves compacting the powder mixture into flakes or ribbons, followed by milling or screening to obtain granules.

Direct Compression: Involves directly compressing the blend of APIs and excipients without prior granulation.

Equipment:

For wet granulation: High shear mixers, fluid bed dryers, and granulators.

For dry granulation: Roller compactors and milling or screening equipment.

For direct compression: Blenders and tablet presses.

Granulating Agents:

Binders: Substances that promote adhesion between particles, such as water, alcohol, or solutions of polymers like hydroxypropyl cellulose (HPC) or polyvinyl pyrrolidone (PVP).

Disintegrants: Aid in the breakup of tablets or capsules in the gastrointestinal tract, ensuring drug release. Examples include croscarmellose sodium and crospovidone.

Process Control:

Granulation process parameters such as mixing time, granulating liquid addition rate, and drying conditions need to be carefully controlled to ensure consistent granule properties.

Monitoring granule size distribution, moisture content, and flow properties is essential for quality control.

Advantages:

Improves flow properties, reducing the likelihood of segregation.

Enhances compressibility, leading to more robust tablets with uniform drug content.

Facilitates controlled release formulations.

Reduces dust generation, improving operator safety and environmental conditions.

Challenges:

Granulation process can be time-consuming and resource-intensive, especially wet granulation.

Risk of over-granulation or under-granulation, leading to issues such as poor flowability or content uniformity.

Some APIs may be sensitive to moisture or heat, requiring careful selection of granulation methods and excipients.

Overall, pharmaceutical granulation is a critical step in the manufacturing of solid dosage forms, ensuring product quality, efficacy, and patient safety.

Sahasrayogam is GMP Certified Granules manufacturer and suppliers in Hyderabad, manufactures 300+ products such as: Softgel, Branded Tablets, Capsules, Syrups, Oils, Powders, Granules and Cosmetics etc.., under Ayurveda, Food & Nutra Ceutics.

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Health supplements

 Our capsules are made of glutinous rice starch, which is gluten free. The name “glutinous” is misleading, as it’s actually gluten free.The name simply comes from the consistency of the rice variety when cooked.

Our capsules are also vegan (many non-vegan capsules contain gelatin, which is an animal byproduct).

We don't use any fillers, as we want to keep our product as natural and pure as possible.

Our product is 100% natural.

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Cellfend is interested in anti-aging and always resending scientific innovations. It learned about autophagy (cellular recycling) as well as figured out how to induce it beyond the advantages of prolonged fasting organic compounds known as polyamines (spermidine is a principal polyamine) are main autophagy induces. So it looked into food supplements that contain polyamines and found that wheat germ is a very high source. Further research showed that with the help of right manufacturing facilities it could create a potent extract of wheat germ for increasing its beneficial aspects and by fermented it then additional compounds are created (known as benzoquinones) that help in improving our health.

 This company is proud to create a high quality product (99%+ purity) in GMP and ISO-9001 certified facilities, that undergoes rigorous third party lab testing.

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A Note About Our Capsules- Our capsules are made of glutinous rice starch, which is gluten free. The name “glutinous” is misleading, as it’s actually gluten free.The name simply comes from the consistency of the rice variety when cooked.

Our capsules are also vegan (many non-vegan capsules contain gelatin, which is an animal byproduct).

We don't use any fillers, as we want to keep our product as natural and pure as possible.

Our product is 100% natural.

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Best Ayurvedic Products Online in India

Since more than 5000 years, Ayurveda has been practiced in India. It is the historical clinical technology. The phrase Ayurveda is a combination of Ayur this means that life and Veda approach medical information. It is rightly known as Science of Life. According to Ayurveda, Health and wellness relies on at the delicate stability among mind, body, and soul. The primary motto of Ayurvedic medication is to promote best health.

SahasraYogam offers best Ayurvedic products. They are herbal and really effective. As the sector is popping closer to Ayurveda, the corporation is likewise attempting and improving the products to make the top ayurvedic products in India.

All Ayurvedic Medicine:

India is the inspiration of Ayurveda. It is the world's oldest clinical system today. The best manner to attain appropriate health is while your body, mind, and soul are in ideal harmony. Each individual is a mixture of five primary factors of space, air, fire, water, and earth as consistent with Ayurveda. The primary basis on which the age-vintage practice of Ayurveda is primarily based totally is genuine in any respect times. Everything changes with time. But the practice of Ayurveda and its concepts have remained steady all through its history. It is the best clinical technology that is steeped in history.

Like the whole thing else changes with time, Ayurveda has advanced over the years. Despite its ancient history, it has miraculously controlled to stay relevant, handy and lower priced to many. Ayurveda is India's number one clinical system. It could pleasantly marvel you to recognize that half of the world's population relies on Ayurvedic remedy and should purchase ayurvedic medicine in online. At a few level for your life, even you will have used grandmothers tried and examined homemade natural medicines. Nowadays you could are seeking clinical recommendation from licensed Ayurvedic practitioners.

Recent History:

There changed into a time in history while Ayurveda nearly have become obsolete. During this period, current western medicinal changed into hastily gaining prominence. The practice of Ayurveda changed into restrained to a small variety of conventional Ayurveda practitioners and the poor. After independence, Ayurveda made a sturdy comeback in India. Today, India is domestic to many famous Ayurvedic schools. Soon the world started out the use of Ayurveda as a clinical technology that heals mind, body, and soul. Many certified Indian Ayurvedic practitioners went to America and Europe to unfold the information of Ayurveda. From then onwards, Ayurveda have become one of the maximum sought-after medicine professions around the world.

New Age of Ayurveda:

In a day and age while the internet is remodelling the manner we speak and live, Ayurveda has penetrated into the remotest corner of the world. Nowadays it's less complicated to shop for ayurvedic medicine online way to the proliferation of internet. It is now feasible to vicinity an order for Ayurvedic medicine online from the consolation of your home, place of business or at the same time as at the go. There are chains of in a competent Ayurvedic shops and clinics that have bloomed during the last few years in India. It has made it less complicated for a character from the remotest corner of India to buy ayurvedic products in online India with none hassle.

Key Benefits of Ayurveda:

Reduces stress and anxiety

Lowers cholesterol level and blood pressure

Facilitates faster healing from accidents and illnesses

Promotes vitamins wealthy and antioxidant wealthy diet

Helps in lowering weight

Reduces inflammation

Helps in preserving hormonal stability

Sahasrayogam is GMP Certified Tablet manufacturer in FSSAI India, manufactures 300+ products such as: Softgel, Branded Tablets, Capsules, Syrups, Oils, Powders, Granules and Cosmetics etc.., under Ayurveda, Food & Nutra Ceutics.

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Ayurvedic Medicine is a Natural Method to Healing

Ayurvedic medicine is a traditional system of medicine that has its roots in ancient India. It is one of the oldest holistic healing systems that focuses on achieving a balance between the body, mind, and spirit to promote overall well-being. Ayurveda, which means "knowledge of life" in Sanskrit, is based on the belief that the body's constitution, or dosha (Vata, Pitta, and Kapha), plays a crucial role in maintaining health and preventing illness.

Key principles of Ayurvedic medicine include:

Doshas: Ayurveda identifies three doshas, which are energies believed to circulate in the body and govern physiological and psychological processes. These doshas—Vata, Pitta, and Kapha—correspond to various functions and elements in the body. Ayurvedic practitioners aim to balance these doshas to maintain health.

Diet and Lifestyle: Ayurveda emphasizes the importance of a balanced diet and lifestyle to maintain harmony within the body. Dietary recommendations are often personalized based on an individual's dosha constitution. In addition to diet, lifestyle practices such as yoga, meditation, and specific daily routines (dinacharya) are considered essential for overall well-being.

Herbal Remedies: Ayurvedic medicine extensively uses plant-based remedies, including herbs and spices, to address various health issues. These remedies are believed to work in harmony with the body and are often prescribed based on an individual's dosha and specific health concerns.

Detoxification (Panchakarma): Ayurveda includes detoxification therapies known as Panchakarma. These treatments aim to eliminate toxins from the body through methods like massage, herbal steam, and purification procedures.

Mind-Body Connection: Ayurveda recognizes the interconnectedness of the mind and body. Emotional well-being is considered crucial for maintaining overall health, and practices like meditation and mindfulness are often recommended to achieve mental balance.

While Ayurvedic medicine has gained popularity for its holistic approach, it's essential to note that its effectiveness may vary, and scientific validation for some of its practices is limited. As with any medical system, it's advisable to consult with qualified practitioners and healthcare professionals, especially if you have existing health conditions.

Before incorporating Ayurvedic treatments, it's crucial to ensure that they align with your overall healthcare plan and receive guidance from a qualified Ayurvedic practitioner or a healthcare professional. Integrating Ayurveda with conventional medicine in a complementary manner, under proper supervision, can provide a holistic approach to health and well-being.

Sahasrayogam is GMP Certified Nutraceutical Liquid Syrup manufacturer in Hyderabad, manufactures 300+ products such as: Softgel, Branded Tablets, Capsules, Syrups, Oils, Powders, Granules and Cosmetics etc.., under Ayurveda, Food & Nutra Ceutics.

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What are The Benefits and Basics of Softgel Capsules

Softgel capsules, also known as soft gelatin capsules, are a popular dosage form used in pharmaceuticals, nutraceuticals, and dietary supplements. They consist of a gelatin-based outer shell that contains a liquid or semi-solid fill. Softgel capsules offer several benefits and are widely used for various reasons. Here are some of the benefits and basics of softgel capsules:

Enhanced bioavailability: Softgel capsules can enhance the bioavailability of certain drugs or nutrients. The liquid or semi-solid fill inside the capsule is readily absorbed by the body, allowing for faster and more efficient absorption compared to other dosage forms.

Easy to swallow: Softgel capsules have a smooth, gelatin-based outer shell that makes them easy to swallow, particularly for individuals who have difficulty swallowing tablets or other solid dosage forms.

Protection and stability: Softgel capsules provide excellent protection and stability for the encapsulated ingredients. The gelatin shell helps to shield the fill from oxidation, moisture, and other environmental factors that may affect its stability and efficacy.

Versatile formulation options: Softgel capsules offer flexibility in formulation design. They can accommodate a wide range of fill materials, including liquids, suspensions, pastes, and even combinations of incompatible substances. This versatility allows for the creation of custom formulations based on specific product requirements.

Improved taste and odor masking: The gelatin shell of softgel capsules helps mask unpleasant tastes and odors of certain drugs or ingredients, making them more palatable for patients or consumers.

Accurate dosing: Softgel capsules can be manufactured with precise dosages, ensuring consistent and accurate delivery of the active ingredients. This is particularly important for medications that require strict dosing regimens.

Branding and differentiation: Softgel capsules can be customized with various colors, shapes, and printing options, allowing companies to differentiate their products and enhance brand recognition.

Extended shelf life: Softgel capsules provide an effective barrier against moisture, oxygen, and light, which helps extend the shelf life of the encapsulated ingredients, ensuring their potency and quality over an extended period.

It's important to note that softgel capsules may not be suitable for all types of drugs or ingredients. Factors such as chemical compatibility, solubility, and stability need to be considered during the formulation process. Additionally, individuals with specific dietary restrictions, such as vegetarians or vegans, may prefer alternative dosage forms, as gelatin is typically derived from animal sources.

Sahasra yogam is GMP Certified company in Hyderabad, manufactures 300+ products such as: Softgel Capsules manufacturers, Branded Tablets, Capsules, Syrups, Oils, Powders, Granules and Cosmetics etc.., under Ayurveda, Food & Nutra Ceutics.

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Why Too Many People are Selecting Ayurvedic Medicines

Too many chronic ailments are on the upward push and there is no health care to counter the spread of some of these illnesses. The end result is that every individual is plagued with a plethora of issues and of their haste to preserve up with everyday life; they come to be popping too many tablets. The health system that currently exists has a totally mechanical technique towards patients and their troubles, for them suppressing a problem is better than absolutely removing the root cause of it.

Though there is a system of medicinal technological know-how that has been in existence for hundreds of years and takes a completely holistic approach in the direction of managing any fitness issue. It takes measures to prevent any diseases along with curing the prevailing ones. Though of course there are a few diseases that do require allopathic medicines so one can be cured but that doesn't reduce the significance of Ayurveda.

This ancient machine of treating sicknesses with herbs and herbal products originated in Hyderabad/India and is surprisingly practiced and recommended. Many countries in the west after the advent of Yoga and different eastern esoteric methods, are taking over the ayurvedic direction to a good health. Ayurveda is a incredibly spiritual and traditional manner of curing a ailment and there are a variety of motives why you must pick to use Ayurvedic medicines:

Holistic approach: Ayurveda is a traditional Indian system of medicine that takes a holistic approach to health and wellness. It focuses on balancing the mind, body, and spirit to promote overall well-being. Ayurvedic medicines are often believed to address the root cause of an ailment rather than just alleviating symptoms.

Natural ingredients: Ayurvedic medicines are typically made from natural ingredients such as herbs, plants, minerals, and oils. Many people prefer these natural remedies as they perceive them to be gentler on the body with fewer side effects compared to synthetic pharmaceutical drugs.

Personalized treatment: Ayurveda emphasizes personalized treatment based on an individual's unique constitution or dosha. This individualized approach aims to restore balance and harmony to the body, considering factors such as one's physical and mental characteristics, lifestyle, and environment.

Long-standing tradition: Ayurveda has a rich history spanning thousands of years and has been used to promote health and treat various ailments. Its longevity and cultural significance have contributed to its popularity and widespread use, particularly among those who have faith in traditional healing systems.

Increasing interest in alternative medicine: In recent years, there has been a growing interest in alternative medicine and natural remedies. People are exploring options beyond conventional pharmaceuticals, seeking more natural and holistic approaches to health. Ayurveda, with its ancient roots and holistic principles, fits into this trend.

Global availability: Ayurvedic medicines have gained international recognition and are increasingly available in various parts of the world. This accessibility has made it easier for people to explore and try Ayurvedic remedies.

It's important to note that while Ayurvedic medicines have their benefits, it's crucial to consult qualified practitioners and ensure the quality and safety of the products used. Additionally, Ayurvedic remedies may not be suitable for all conditions, and it's essential to combine them with appropriate medical care when necessary.

Sahasrayogam is GMP Certified Softgel Capsules manufacturer in Hyderabad, manufactures 300+ products such as: Softgel, Branded Tablets, Capsules, Syrups, Oils, Powders, Granules and Cosmetics etc.., under Ayurveda, Food & Nutra Ceutics.

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The Benefits of Ayurvedic Medicine in Today's World

Ayurveda, the ancient Indian system of medicine, has been around for thousands of years. In recent years, it has gained popularity in the Western world as people seek natural remedies and holistic approaches to health. Ayurveda is based on the idea that each person has a unique constitution, or dosha, and that health is achieved through balancing the doshas.

At our Ayurvedic manufacturing company, we believe in the power of Ayurvedic medicine to promote wellness and prevent disease. Here are some of the benefits of Ayurvedic medicine in today's world:

Personalized approach: Ayurveda recognizes that each person is unique and that there is no "one-size-fits-all" approach to health. By understanding your dosha, you can tailor your lifestyle, diet, and herbal remedies to your specific needs.

Natural remedies: Ayurvedic medicine uses natural remedies such as herbs, spices, and oils to promote health and wellness. These remedies are gentle on the body and have few side effects compared to pharmaceuticals.

Holistic approach: Ayurveda considers the whole person - body, mind, and spirit - in promoting health. By addressing all aspects of a person's health, Ayurveda can help prevent disease and promote overall well-being.

Time-tested tradition: Ayurveda has been around for thousands of years and has a long history of successful treatment of various health conditions. This time-tested tradition provides a level of trust and confidence in the effectiveness of Ayurvedic medicine.

At our Ayurvedic manufacturing company, we are committed to producing high-quality Ayurvedic products that are safe, effective, and affordable. We believe that Ayurveda has much to offer in today's world, and we are proud to be a part of this ancient tradition.

Whether you are looking for natural remedies to support your overall health or seeking treatment for a specific health condition, Ayurvedic medicine can provide a personalized and holistic approach to wellness. Contact us to learn more about our Ayurvedic products and how they can support your health and well-being.

Sahasrayogam is GMP Certified Ayurvedic Granules Manufacturer in Hyderabad, manufactures 300+ products such as: Softgel, Branded Tablets, Capsules, Syrups, Oils, Powders, Granules and Cosmetics etc.., under Ayurveda, Food & Nutra Ceutics.