#alexander echeverri

[commedessgarcons]

Normani for Who What Wear’s cover story, Ph. by Ahmad Barber + Donte Maurice

Styling by Lauren Eggertsen

Hair by Ashanti Lation

Makeup by Alexander Echeverri

Art direction by Amy Armani

Designer:Ally Quirk

[thejaguartour]

🖤 Grammy week with Victoria Monét 🤎

👗: Laquan Smith

📸: Dalvin Adams

💄: Alexander Echeverri

[cozyaliensuperstar7]

Queens 👑👑👑👑👑

#Repost @victoriamonet

——

Cover girl for @clashmagazine issue 125 🐆🤎✨

Photographer: Jamie Bruce - @byjamiebruce

Styling: Timothy Luke Garcia - @timothyluke_

Hair: Davontae Washington - @iamdavontae

Braids: Bryson Karter - @brysonkarter

MU: Alexander Echeverri - 

@mua.alexander

Words: Shahzaib Hussain - @shazzasherazi

Cover Creative Direction: Rob Meyers @rbpmstudio

#Repost @chloebailey

——

bad ting 💋

#Repost @keke

——

My boo @victoriamonet told me to throw on some brown and meet her in the studio. I said… I’m on my way 😌😆

[lenzwizard]

Older Man from Alexander Films LLC on Vimeo.

Full Sail University project for month 5, Experimental Filmmaking. For the assignment, I chose an exercise in dialogue -- rhythm and flow of speech and conversation -- tackling inter-gender, inter-age, and inter-cultural differences. LANGUAGE WARNING!

Cast: Lexie Smith – ABBY Hayley Haas – BECKY Aaron Sherry – WILLIAM

Crew: Marc A Hutchins – writer, director, producer, editor, colorist, title, credits, music supervisor, casting director Rhashad Griffin – producer, asst. casting director Brent Wilkie – location sound mixer, boom op, key grip JP Eason – gaffer Miiki Shay – script supervisor Javier Rojas Lavalle – AC, grip Jose Echeverry – 2AC, grip

Music courtesy of Amy May Music LLC

Filmed on location in the Full Sail MFA soundstage

Camera and lens: RED Scarlet-X with Canon EF mount and Rokinon primes

Audio: Roland R-44 (compact 4-channel mixer/recorder) with an Octava MK-012-01 condenser mic on a K-Tek boom

Edited on: Adobe Premiere Pro CC 2017

Colored with: Magic Bullet Looks

[dimensiontotal]

𝐋𝐮𝐧𝐞𝐭𝐚 𝟓𝟎 𝐩𝐫𝐞𝐬𝐞𝐧𝐭𝐚: “𝐂𝐮𝐞𝐧𝐭𝐨 𝐋𝐢𝐛𝐫𝐞”

Después de la pandemia del covid19 que obligó a la humanidad a confinarse en sus hogares, la sala Luneta 50 ubicada en la Carrera 63 Número 58 – 44, le sigue apostándole a la con su programación especial dirigida a público infantil, y este sábado 23 de octubre del 2021 a partir de las 4:00 de la tarde se presentará al grupo de teatro Camach con el espectáculo “Cuento Libre”, una divertida pieza teatral en la que se mezclan distintas expresiones de las artes escénicas.

La obra es la ganadora de la convocatoria de Estímulos 2017 de la Secretaría Distrital de Cultura y Turismo de Barranquilla, presenta la adaptación de tres historias infantiles escritas por María Walsh, poetisa y cantautora argentina, considerada como un prócer cultural. Su obra revolucionó la manera en que se entendía la relación entre poesía e infancia; y muchos críticos han señalado que lo escrito por María Elena Walsh es comparable a Alicia en el país de las maravillas, de Lewis Carrol, o Pinocho, de Carlo Collodi.

El grupo Camach, colectivo de teatro de Barranquilla, fundado en 2014 y dirigido por el maestro en Arte Dramático Wilfer Donado e integrado por los actores Patricia Gaviria y Libardo Echeverry, y los músicos Félix Viáfara y Mingo Sánchez.

El propósito de estos jóvenes artistas es indagar, reflexionar y crear espectáculos que muestran las múltiples formas expresivas del teatro tales como los títeres, la narración oral y la danza.

Por: Alexander Lewis Delgado.

Foto: @luneta50

[unoxunoagencia]

“COMO FLOR EN LA MALEZA”

STILLS

Un Film de Mccalle @mccallefilm

Canción por The Virginia Valley @thevirginiavalley

Casting Tanner Resse @tannerareese  ·  Tom Santiago Zapata @santogozj  ·  Jack Vladímir Bernal @vladimirbernalactor  ·  Max/ Papá Mónica Echeverri @monicaecheverrie  ·  Dianne Taylor/ Mamá

Producción  ·  Unoxuno @unoxuno Producción Ejecutiva  ·  Americanino @americanino & Unoxuno @unoxuno Producción Ejecutiva  ·  Alejandra Laverde @alejamanga Productor en línea  ·  Santiago Arbelaez @sao_2111 Productor de campo  ·  Alejandro Ríos @alejomellorios Asist. Produccion  ·  Juan Camilo Álvarez  @juragui 1er Asist. Vestuario  ·  Jonathan Correa @jonathancvargas 2do Asist. Vestuario  ·  Benyair Fernández @benji_xq28

Director de fotografía  ·  Luciana Riso @lucianariso 1er AC  ·  Felipe Sanz @focosanz 2do AC  ·  Zanvo @zanvo Steadycam  ·  Daniel Santoyo @danielsantoyo.d Asist.Steadycam  ·  Sebastian Ramón @sebastian_ramon Gaffer  ·  Henry Vidal @dalvvvvvv Luminotécnico  ·  Samir Meluk @meluksmh Eléctrico  ·  Julian Andrés Echavarría @negroblancoso Asist. Luces  ·  Nilson Andrés Flores @nilsonfloo Asist. Luces  ·  Daniel Rodriguez @crewdniel10 Asist. Luces  ·  Maycol Vélez @velezmaycol Asist. Luces  ·  Alexander “Buggy”@buggymorales Asist. Luces  ·  Marcelo Oquendo @marcelo_oquendo

Asist. Dirección  ·  Nataly Valdivieso @natyvaldi0101 Dirección de Arte  ·  Artx Escenografía @artx_scenography 1er Director de Arte  ·  Felipe Vásquez @felipevasq 2do Director de Arte  ·  Simón Cataño @simoncastanog 1er Asist. Arte  ·  Sebastián Ferraro @sferraro7 2do Asist. Art  ·  Luz Celeste Lopera @luzcelesti 3er Asist. Art  ·  Juan Camilo Fernández @juan_23_fernandez Coreógrafo  ·  Felipe Arias (Fear) @fear.chr

M&H  ·  Veronica Simson @dvsimson M&H  ·  Catalina Uribe @catalinapromua M&H  ·  María Inés Aguirre @itsmiamakeup

Edición  ·  Esteban Aldana @estebanaldana_s Color  ·  Freddy Mejía @freddymejia24 VFX  ·  Office creativa @officecreativa Gráfico  ·  Fel Gonzales @lefel__ @lifeblood.est.696

Foto Fija/ Detrás de Cámara  ·  Sebastián Mesa @s__mesa Fotógrafo  ·  Cristian Rubbot @ruboott

Mix  ·  Pablo Melov @pamelov Mastering  ·  Sebastián Lopera @sebastianmastering Composición  ·  Tattiana Echeverri@letatt /Pablo Melov Diseño Sonoro  ·  Checho Estrada @chechoestradad Diseño Sonoro  ·  Esteban Aldana @estebanaldana_s

Catering  ·  A la mesa @catering_alamesa

Revelado y Escaneado  ·  Laboratorio Fílmico LABO @labooficial

Agradecimientos especiales a LOMA Film Rental @lomafilmrental

VV

[solatgif]

TGIF: ROUNDUP FOR JANUARY 22, 2021

SOLA Network Editor Hannah Chao and I got together to talk about The Best Articles from SOLA Network 2020. Watch on YouTube. If you have an article or link to share, reach me on Twitter or Instagram.

Should all churches be multiethnic? Is there still a place for ethnic-specific churches? Moses Y. Lee spoke with Thabiti Anyabwile and Dr. Alexander Jun, and we will share their conversation on Monday. Watch a preview on YouTube.

Our first SOLA Network Magazine features our best articles from 2020. Read it online as a flipbook or as a PDF download for free. Read and subscribe to our monthly newsletter and see our most popular articles. For more of our faves, check out SOLA’s Hidden Gems of 2020.

ARTICLES FROM AROUND THE WEB

1. Jay Y. Kim: Inaugurations and Image Bearing

“While many Christians might bear the name of Christ, it isn’t clear that they bear his image as their truest source of identity and allegiance.”

2. Vaneetha Rendall Risner: Has God Withheld Good from Me?

“Christians should never mistake fame, money, and health for the best things of God.”

3. Kevin DeYoung: We Must Find a Better Way to Talk About Race

“The simple, honest truth is that Bible-believing orthodox Christians are not setting a Spirit-infused example in how to talk about racial matters.”

BOOKS, PODCASTS, MUSIC, AND MORE

1. Brett McCracken: Three Habits Making Us Sick

Three particular dynamics of the information age that are making us foolish and sick: Too much, Too fast, Too focused on me.

2. TGC Q&A: How Do the Arts Help Us Learn and Grasp Scripture?

Josh Byers and Quina Aragon on the arts as a means of communication, Imago Dei, and more.

3. Aaron Lee: Related Works

Book Reviews: The God Contest by Carl Laferton and Catalina Echeverri; Evil and Creation by David Luy, Matthew Levering, and George Kalantzis. Commentary Review: Hebrews by Thomas R. Schreiner. Our TGIF playlist is available on Spotify. Join my Asian American Worship Leaders Facebook group.

FEATURED THIS WEEK ON SOLA NETWORK

1. Thabiti Anyabwile, Alexander Jun, and Moses Y. Lee: SOLA Preview: Why Ethnic-Specific Churches are Still Important

“Being in ethnic-specific context is not only permissible, it's actually quite necessary for a lot of Christians to receive the comfort and discipleship that that's needed.”

2. Hannah Chao and Aaron Lee: The Best Articles from SOLA Network 2020

“I wanted to show that SOLA is here to equip Christians of all age ranges and on so many different topics.”

3. Daniel K. Eng: Tips for Reading and Teaching the Book of Ruth

“It is because of the lovingkindness of Christ that we who follow him can have a future.”

4. Hannah Chao: Remembering Dr. King's Words from a Birmingham Jail

“We pray that his words will continue to challenge us as we fight for justice for and through the Gospel.”

5. TGIF: Roundup for January 15, 2021

10 Commitments for 2021 / Let’s Be Different in 2021. (But Not Like That.) / The Day We Saw the Capitol Fall

General disclaimer: Our link roundups are not endorsements of the positions or lives of the authors.

[naivelocus]

Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated

Highlights

•

Cryo-EM shows human cytoplasmic dynein-1 in its auto-inhibited, phi-particle form

•

Phi-particle disruption in vitro and in cells reveals its role in dynein regulation

•

There is a transition from phi-particle to open-dynein: both forms are inhibited

•

Dynactin binds open-dynein and aligns its motors to activate processive movement

Summary

Cytoplasmic dynein-1 binds dynactin and cargo adaptor proteins to form a transport machine capable of long-distance processive movement along microtubules. However, it is unclear why dynein-1 moves poorly on its own or how it is activated by dynactin. Here, we present a cryoelectron microscopy structure of the complete 1.4-megadalton human dynein-1 complex in an inhibited state known as the phi-particle. We reveal the 3D structure of the cargo binding dynein tail and show how self-dimerization of the motor domains locks them in a conformation with low microtubule affinity. Disrupting motor dimerization with structure-based mutagenesis drives dynein-1 into an open form with higher affinity for both microtubules and dynactin. We find the open form is also inhibited for movement and that dynactin relieves this by reorienting the motor domains to interact correctly with microtubules. Our model explains how dynactin binding to the dynein-1 tail directly stimulates its motor activity.

Keywords

motor;

dynein;

dynactin;

cryo-EM;

microtubule;

phi-particle;

auto-inhibition;

activation

Introduction

Cytoplasmic dynein-1 (dynein) associates with dynactin to form an efficient microtubule motor that transports cargo to the minus end of microtubules and organizes the internal components of eukaryotic cells. Disruption of this 2.4-megadalton machine disperses the Golgi network (Burkhardt et al., 1997), blocks transport between organelles (Presley et al., 1997), and leaves viruses stuck at the cell periphery (Döhner et al., 2002). In addition, dynein and dynactin are required during cell division for spindle formation and correct chromosome alignment (Echeverri et al., 1996). Dynein must therefore be carefully regulated to ensure the correct timing and location of motor activation.

In cells, most dynein is diffuse in the cytoplasm, with only a small fraction on microtubules (Splinter et al., 2012). This prevents dynein from inappropriately saturating microtubules or traveling unnecessarily and ensures there is a pool of dynein ready to transport cargos when required. The switch of dynein and dynactin from diffuse to actively transporting cargo is controlled at many levels. It can be driven both in vitro (Mallik et al., 2005) and in vivo (Rai et al., 2013) by clustering motors and influenced by targeting dynein/dynactin to specific post-translationally modified microtubules (McKenney et al., 2016 ;  Nirschl et al., 2016) or the microtubule plus ends (Duellberg et al., 2014 ;  Moughamian et al., 2013). The switch is also controlled at the level of the dynein/dynactin machinery itself. Whereas isolated human dynein is weakly processive in vitro (Trokter et al., 2012), it can be activated to move over long distances (>500 nm) by binding to dynactin and a cargo-specific adaptor protein such as BICD2 (McKenney et al., 2014 ;  Schlager et al., 2014) or Hook3 (McKenney et al., 2014; Olenick et al., 2016 ;  Schroeder and Vale, 2016). This binding stimulates processive movement by increasing the run length, velocity (McKenney et al., 2014 ;  Schlager et al., 2014), and force output (Belyy et al., 2016) of individual motors.

Dynein consists of two motor domains that are responsible for ATP hydrolysis and force production and a tail region that holds them together. It is unclear why dynein is only weakly processive on its own and how it is activated by dynactin and cargo adaptors. There is some evidence that dynein processivity is directly inhibited by the C-terminal ∼300 amino acids of the motor domain (Nicholas et al., 2015). Another theory is that inhibition is due to the tail region folding back to contact the motor domains until cargo binds (Belyy et al., 2016 ;  Markus et al., 2009). A similar inhibition mechanism is used by cytoskeletal motors in the kinesin (Kaan et al., 2011) and myosin families (Hammer and Sellers, 2011). Alternatively, it has been proposed that dynein is auto-inhibited by self-dimerization of its motor domains (Torisawa et al., 2014). This form of dynein is referred to as the phi-particle because of its resemblance to the Greek letter phi (φ) (Amos, 1989). Activation was suggested to result from a shift in the equilibrium of dynein conformations toward an open form in which the motor domains are separated. In support of this, forced separation of isolated dynein motor domains can increase motor activity (Torisawa et al., 2014). However, these studies were performed on artificially dimerized dynein motors lacking the tail region. It is therefore not clear whether the tail contributes to inhibition or what role the phi-particle plays in the context of the whole dynein complex.

In this study, we set out to determine whether the phi-particle contributes to dynein auto-inhibition and how dynein is activated by dynactin. We use cryoelectron microscopy (cryo-EM) to determine the structure of the phi-particle. We show how the motor domains self-dimerize and are locked in a conformation with weak affinity for microtubules. Disrupting the motor dimer by structure-based mutagenesis drives dynein into an open form with increased affinity for microtubules and dynactin. Surprisingly, we discover that the open form of dynein is also inhibited. We use a combination of 2D analysis of EM images and a 3D cryo-EM structure of the whole dynein/dynactin machinery to explain how dynactin overcomes this inhibition and directly reorients the motor domains to make dynein processive. Finally, we show that disrupting the phi-particle in cells causes mis-localization and mitotic defects, supporting a physiological role for the phi-particle in dynein regulation.

Author : Kai Zhang, Helen E. Foster, Arnaud Rondelet, Samuel E. Lacey, Nadia Bahi-Buisson, Alexander W. Bird, Andrew P. Carter

— Cell

[mererandomness]

[thejaguartour]

Victoria Monét & Hazel Monét with that Grammy-winning Glow ✨🌟✨

📸: Dalvin Adams

👗: Versace

💎: Bulgari

💄: Alexander Echeverri

💇🏾‍♀️: Davontaé Washington

[thejaguartour]

Victoria Monét celebrating Grammy Week 🤎

👗: Laquan Smith

📸: Dalvin Adams

💄: Alexander Echeverri

[thejaguartour]

Victoria Monét’s look for Grammy Nominee Conversations at the Grammy Museum (ft. Mommy Monét) 🤎🤍🐾

📸: D. Adams

💄: Alexander Echeverri

💇🏾‍♀️: Ke

🧥: Kollin Carter — Area, Jimmy Choo, and Alexis Bittar Jewelry

[thejaguartour]

Victoria Monét, John Gaines, Mama Monét & her step-father celebrating Grammy Week 🤎

📸: Dalvin Adams

👗: Laquan Smith

💄: Alexander Echeverri

[thejaguartour]

Victoria Monét as a beach baddie in Brazil 🧡

📸: Tyler Fuhrmeister

💄: Alexander Echeverri

💇🏾‍♀️: Fellipe Parks

👙: Kollin Carters

[thejaguartour]

Victoria Monét • Clash Magazine • Issue 125

Credit to Jamie Bruce, Timothy Luke Garcia, Davonte Washington, Alexander Echeverri, Shahzaib Husain, and Rob Meyers

[unoxunoagencia]

“COMO FLOR EN LA MALEZA”

Un Film de Mccalle @mccallefilm

Canción por The Virginia Valley @thevirginiavalley

Casting Tanner Resse @tannerareese  ·  Tom Santiago Zapata @santogozj  ·  Jack Vladímir Bernal @vladimirbernalactor  ·  Max/ Papá Mónica Echeverri @monicaecheverrie  ·  Dianne Taylor/ Mamá

Producción  ·  Unoxuno @unoxuno Producción Ejecutiva  ·  Americanino @americanino & Unoxuno @unoxuno Producción Ejecutiva  ·  Alejandra Laverde @alejamanga Productor en línea  ·  Santiago Arbelaez @sao_2111 Productor de campo  ·  Alejandro Ríos @alejomellorios Asist. Produccion  ·  Juan Camilo Álvarez  @juragui 1er Asist. Vestuario  ·  Jonathan Correa @jonathancvargas 2do Asist. Vestuario  ·  Benyair Fernández @benji_xq28

Director de fotografía  ·  Luciana Riso @lucianariso 1er AC  ·  Felipe Sanz @focosanz 2do AC  ·  Zanvo @zanvo Steadycam  ·  Daniel Santoyo @danielsantoyo.d Asist.Steadycam  ·  Sebastian Ramón @sebastian_ramon Gaffer  ·  Henry Vidal @dalvvvvvv Luminotécnico  ·  Samir Meluk @meluksmh Eléctrico  ·  Julian Andrés Echavarría @negroblancoso Asist. Luces  ·  Nilson Andrés Flores @nilsonfloo Asist. Luces  ·  Daniel Rodriguez @crewdniel10 Asist. Luces  ·  Maycol Vélez @velezmaycol Asist. Luces  ·  Alexander “Buggy”@buggymorales Asist. Luces  ·  Marcelo Oquendo @marcelo_oquendo

Asist. Dirección  ·  Nataly Valdivieso @natyvaldi0101 Dirección de Arte  ·  Artx Escenografía @artx_scenography 1er Director de Arte  ·  Felipe Vásquez @felipevasq 2do Director de Arte  ·  Simón Cataño @simoncastanog 1er Asist. Arte  ·  Sebastián Ferraro @sferraro7 2do Asist. Art  ·  Luz Celeste Lopera @luzcelesti 3er Asist. Art  ·  Juan Camilo Fernández @juan_23_fernandez Coreógrafo  ·  Felipe Arias (Fear) @fear.chr

M&H  ·  Veronica Simson @dvsimson M&H  ·  Catalina Uribe @catalinapromua M&H  ·  María Inés Aguirre @itsmiamakeup

Edición  ·  Esteban Aldana @estebanaldana_s Color  ·  Freddy Mejía @freddymejia24 VFX  ·  Office creativa @officecreativa Gráfico  ·  Fel Gonzales @lefel__ @lifeblood.est.696

Foto Fija/ Detrás de Cámara  ·  Sebastián Mesa @s__mesa Fotógrafo  ·  Cristian Rubbot @ruboott

Mix  ·  Pablo Melov @pamelov Mastering  ·  Sebastián Lopera @sebastianmastering Composición  ·  Tattiana Echeverri@letatt /Pablo Melov Diseño Sonoro  ·  Checho Estrada @chechoestradad Diseño Sonoro  ·  Esteban Aldana @estebanaldana_s

Catering  ·  A la mesa @catering_alamesa

Revelado y Escaneado  ·  Laboratorio Fílmico LABO @labooficial

Agradecimientos especiales a LOMA Film Rental @lomafilmrental

VV