#cdk response

[aealzx]

Hi

not to disturb you

But I would like to ask when the next part of the tmnt 2003 and Rottmnt crossover will come out

I’m not rushing you I just want to know when I should look forward to it

Because it is one of the best fanfiction/crossover story/whatever the hell you want to call it I have ever read

I honestly hadn't realized it'd been this long since I updated CDK 8'D I have the worst sense of time.

I actually wrote the next 2 parts like.....last week sometime, just a couple more sentences or so. I've just been distracted drawing others things and being too scared to try and draw Leatherhead's face. It was on my mental queue to do after I finish this giftart I'm currently doing, and I usually post it as soon as I finish both parts. So...... 2 or 3 days? Depending on how much I struggle with Leatherhead and stuff.

Thank you though X'D I'm really surprised how many people like it so much.

[aitoolswhitehattoolbox]

AWS Security Engineer+python+CDK

: Bengaluru skills: AWS security engineer with Python & CDK Build a career with confidence: Carrier Global Corporation… scripting (e.g., Python) and DevOps practices. He/she will be responsible for ensuring the security of our cloud… Apply Now

[news2024news]

CDK Cyberattack: What Is It, Who Is Responsible and What’s the Fallout? http://dlvr.it/T8hnGX

[flentas]

Streamlining Continuous Integration with AWS DevOps Services and Consulting 

Efficiency and agility are paramount in the world of software development. Companies aim to remain ahead in the competitive market and search for solutions that can improve their development processes. This is where Continuous Integration (CI) comes into play. CI, a cornerstone of modern software development practices, ensures that code changes are integrated into the main codebase frequently and automatically. However, implementing CI effectively requires more than just tools; it demands a comprehensive approach that AWS DevOps services and consulting can significantly facilitate. As technology accelerates, companies need a strategic partner like Flentas, an AWS consulting partner providing AWS DevOps services, to streamline their CI practices and optimize their development pipelines. 

The Evolution of Continuous Integration 

Continuous Integration emerged as a response to the challenges posed by traditional software development approaches. In the past, teams often worked in isolation, leading to the accumulation of code changes that were only integrated periodically. This resulted in integration conflicts, longer debugging cycles, and delayed project deliveries. Continuous Integration addressed these issues by advocating for integrating code changes into the central repository multiple times daily. This approach ensures that integration conflicts are detected and resolved early, leading to smoother development cycles and faster delivery of features. 

The Role of Automation in CI/CD 

Automation is a fundamental CI/CD (Continuous Integration/Continuous Deployment) pipeline principle. It reduces manual intervention and ensures consistency in the deployment process. The AWS DevOps services provide various automation tools that can effortlessly be integrated into the development pipeline. Infrastructure as Code (IaC) tools like AWS CloudFormation and AWS CDK enable teams to define and provision infrastructure using code. This approach enhances reproducibility and reduces the chances of configuration drift between different environments. 

Additionally, AWS CodePipeline and AWS CodeBuild automate the pipeline's build, test, and deployment stages. These services allow developers to define custom workflows that suit their application requirements. By automating these crucial steps, development teams can focus on writing code and designing features instead of manually managing deployment processes. 

Enhanced Testing with AWS DevOps Services 

The testing phase is a crucial part of the software development process. It ensures that the code is of high quality and functions as intended. AWS provides several services that facilitate comprehensive testing strategies. AWS CodeBuild supports various testing frameworks, enabling automated unit, Integration, and performance tests. This Integration ensures that any code changes introduced do not adversely affect the application's existing functionality. 

Moreover, AWS Device Farm enables testing on real devices across different platforms, ensuring the application behaves consistently across various devices and operating systems. By leveraging AWS DevOps services, organizations can create robust testing pipelines that deliver reliable results and reduce the likelihood of bugs reaching production environments. 

Flentas: Your Partner in CI/CD Transformation 

In the dynamic realm of DevOps and CI/CD, partnering with a knowledgeable and experienced service provider is crucial. Flentas, an AWS consulting partner offering AWS DevOps services, brings a wealth of expertise to the table. With a deep understanding of AWS tools and best practices, Flentas assists organizations in tailoring CI/CD pipelines that align with their specific needs. 

Flentas' consultants collaborate closely with development teams, identifying bottlenecks, refining processes, and implementing automation to streamline the CI/CD workflow. This partnership empowers businesses to achieve faster time-to-market, reduced development costs, and enhanced software quality. By leveraging Flentas' expertise, organizations can transition smoothly to modern development practices and fully realize the benefits of CI/CD. 

Conclusion 

Continuous Integration remains a cornerstone of success in the ever-evolving landscape of software development. Organizations prioritizing efficient CI practices position themselves for rapid innovation, reduced time-to-market, and improved software quality. To achieve these goals, it's not enough to simply use tools. A strategic approach is needed that integrates automation, testing, and expertise. 

Flentas, an AWS consulting partner offering AWS DevOps services, plays a pivotal role in this journey. Through collaboration and expertise, Flentas empowers organizations to optimize their CI/CD pipelines, accelerate development cycles, and deliver exceptional software products. In the midst of the challenges of modern software development, teaming up with Flentas can be a strategic advantage for businesses, helping them succeed in the digital age. 

For more details about our services, please visit our website – Flentas Technologies 

[deploypolygoncdkdeploymentstuff]

Empowering Developers: Unleashing the Potential of Polygon CDK Deployment for Blockchain Innovation

Blockchain technology has revolutionized the way we conceptualize and implement decentralized solutions. However, despite its potential, developing blockchain applications has traditionally been complex and challenging, often requiring specialized knowledge and expertise. In response to these challenges, platforms like Polygon have emerged to provide developers with the tools and resources needed to simplify the development process and unleash the full potential of blockchain innovation. One such tool is the Polygon Chain Development Kit (CDK), a comprehensive suite of tools, frameworks, and libraries designed to streamline the development and Polygon CDK deployment of blockchain applications on the Polygon network.

At its core, the Polygon CDK aims to empower developers by abstracting away the technical complexities of blockchain development, allowing them to focus on building innovative solutions without being encumbered by the intricacies of blockchain protocols. By providing a user-friendly interface and intuitive documentation, the Polygon CDK lowers the barrier to entry for developers of all skill levels, enabling them to start building blockchain applications quickly and efficiently.

One of the key features of the Polygon CDK is its support for Ethereum Virtual Machine (EVM) compatibility, which allows developers to leverage their existing knowledge and expertise in Ethereum development. This interoperability enables developers to seamlessly port their Ethereum smart contracts and dApps to the Polygon network, taking advantage of its superior scalability and lower transaction costs. By providing a familiar development environment, the Polygon CDK accelerates the adoption of Polygon as a preferred platform for blockchain development, attracting a broader community of developers to build on its network.

Furthermore, the Polygon CDK offers a rich set of tools and frameworks for building and deploying decentralized applications (dApps) tailored to a wide range of use cases. Whether developers are building decentralized finance (DeFi) protocols, non-fungible token (NFT) marketplaces, gaming platforms, or supply chain solutions, the Polygon CDK provides the necessary tools and resources to bring their ideas to life. From smart contract development frameworks to interoperability solutions and developer-friendly APIs, the Polygon CDK empowers developers to explore new possibilities and push the boundaries of what is possible in blockchain innovation.

Additionally, the deployment of the Polygon CDK enables developers to take advantage of Polygon's Layer 2 scaling solutions, such as zkRollups and Optimistic Rollups. These scaling solutions allow developers to process transactions off-chain before submitting them to the Ethereum mainnet, significantly increasing transaction throughput and reducing gas fees. By integrating these scaling solutions into their dApps, developers can deliver fast and cost-effective experiences to users, making blockchain applications more accessible and user-friendly.

Moreover, the deployment of the Polygon CDK fosters collaboration and innovation within the blockchain community, creating a vibrant ecosystem of developers, projects, and ideas. Through hackathons, workshops, and developer forums, the Polygon community provides a platform for developers to share knowledge, collaborate on projects, and learn from one another. This culture of collaboration and knowledge-sharing accelerates the pace of innovation and drives the evolution of blockchain technology, paving the way for a more decentralized and interconnected future.

In conclusion, the deployment of the Polygon CDK represents a significant milestone in the evolution of blockchain development, empowering developers to unleash the full potential of blockchain innovation. By providing a comprehensive suite of tools, frameworks, and resources, the Polygon CDK simplifies the development process, enhances scalability, and fosters collaboration within the blockchain community. As more developers embrace the Polygon CDK and explore its capabilities, the future of blockchain innovation looks brighter than ever, with a more inclusive, efficient, and decentralized ecosystem that empowers developers to build the next generation of blockchain solutions.

[deploypolygonsdkstuff]

Unleashing the Power: A Comprehensive Guide to Setting Up and Optimizing Your Polygon CDK Node

In the dynamic realm of blockchain development, setting up and optimizing a Polygon CDK (Contract Development Kit) node is a fundamental step for developers and enthusiasts looking to actively participate in the Polygon network. Polygon, with its emphasis on scalability and developer-friendly environment, provides a robust ecosystem for deploying decentralized applications (DApps). This comprehensive guide aims to empower individuals by providing a step-by-step walkthrough on setting up and optimizing a Polygon CDK node, unleashing its power for seamless blockchain development.

Understanding the Role of a Polygon CDK Node

A Polygon CDK node plays a crucial role in the Polygon network. Nodes are responsible for maintaining the decentralized nature of the blockchain, participating in the consensus mechanism, and ensuring the integrity of transactions. By setting up and optimizing a CDK node, individuals contribute to the security, reliability, and efficiency of the Polygon network.

1. Initial Setup: Installing the Necessary Software

The first step in unleashing the power of a Polygon CDK node involves the initial setup. Developers need to install the necessary software on their system. Polygon CDK is designed to be compatible with popular development frameworks such as Truffle and Hardhat. Installing these frameworks ensures a smooth integration with the Polygon network and simplifies the deployment of smart contracts.

2. Configuring the CDK Node

Configuration is a critical aspect of setting up a Polygon CDK node. Developers should pay attention to parameters such as network settings, gas configurations, and security measures. Configuring the CDK node correctly ensures seamless communication with the Polygon network and optimizes the node's performance for the specific requirements of decentralized application development.

3. Establishing Network Connectivity

Connecting the CDK node to the Polygon network is a crucial step in unleashing its power. Developers can choose to connect to the Polygon testnet for initial testing and development or directly to the mainnet for production deployments. Establishing network connectivity ensures that the CDK node actively participates in the Polygon network, contributing to the overall consensus mechanism.

4. Security Best Practices

Security is paramount in the blockchain space, and optimizing a Polygon CDK node involves implementing robust security measures. Developers should follow best practices for securing their nodes, including regular software updates, firewalls, and secure access controls. Ensuring the security of the CDK node not only protects the developer's assets but also contributes to the overall security of the Polygon network.

5. Performance Optimization Techniques

Optimizing the performance of a Polygon CDK node is essential for efficient blockchain development. This includes optimizing resource utilization, implementing caching strategies, and configuring the node for optimal transaction processing. Performance optimization ensures that the CDK node operates seamlessly, contributing to a reliable and responsive blockchain infrastructure.

6. Continuous Monitoring and Maintenance

Once the CDK node is set up and optimized, it requires continuous monitoring and maintenance. Developers should implement monitoring tools to track the node's health, transaction processing speed, and resource utilization. Continuous maintenance involves staying updated with the latest software releases, addressing any performance issues promptly, and ensuring the node's reliability over time.

Conclusion: Empowering Developers in the Polygon Ecosystem

In conclusion, setting up and optimizing a Polygon CDK node is a pivotal step for developers seeking to unleash the full potential of the Polygon network. By following this comprehensive guide, individuals can empower themselves to actively participate in the decentralized future of blockchain development. A well-configured and optimized CDK node not only contributes to the security and reliability of the Polygon network but also provides developers with a powerful tool for deploying and testing their decentralized applications seamlessly. As the blockchain ecosystem continues to evolve, individuals with the knowledge to set up and optimize Polygon CDK nodes are well-positioned to be at the forefront of decentralized application innovation.

[metamoonshots]

Canto, a Cosmos-based layer1 community, plans emigrate to Ethereum (ETH) and turn into a layer-2 community.Following the information, the CANTO token jumped by practically 13% to $0.1285 as of press time, in accordance with CryptoSlate’s knowledge.Canto to construct on Polygon.Canto core builders will construct a zero-knowledge rollup on Ethereum Layer-2 utilizing the Polygon (MATIC) Chain Improvement Package. Polygon Labs says Canto can faucet “the liquidity of a unified Polygon ecosystem with easy accessibility to Ethereum.”“By becoming a member of a rising variety of chains that extends blockspace, the transfer displays a big step towards a “neofinance” future, or the protocolization of current finance,” Polygon added.Polygon co-founder Sandeep Nailwal expressed delight with the transfer, noting that Canto’s migration would “turbocharge the neofinance revolution.” He added:“The main focus of a ZK-migrated Canto L2 is neofinance – bringing off-chain property onto crypto rails. However to do that, the Canto group wants to have the ability to faucet Ethereum’s liquidity in a trustless and safe manner, with out compromise. A Canto L2 will characteristic a trustless ZK bridge to Ethereum, out-of-the-box.On the similar time, Canto retains its L1 sovereignty, even because the group scales its core ethos of liquidity as a public good.”Different networks, together with Astar, Palm, IDEX, and Gnosis Pay, have made comparable strikes to construct ZK rollups on Ethereum’s second layer utilizing Polygon CDK. Nailwal said, “the plan is for all CDK-powered chains to be interconnected and interoperable, with entry to the shared liquidity of all different chains.”Layer2 networks specializationThe migration of a number of layer-1 networks to Ethereum’s second layer is concurrent with the maturation of the layer-2 ecosystem. New specialised networks are rising in response to those developments.Blockchain analytical agency IntoTheBlock reported that main layer-2 networks are experiencing progress in distinct domains.Optimism (OP), for example, is carving a distinct segment for itself as a superchain. Many networks are adopting the OP stack for his or her layer-2 options. In the meantime, Polygon and Arbitrum (ARB) actively help scaling options by their respective applied sciences.In decentralized finance (DeFi), Arbitrum stands out as a dominant drive, boasting the very best liquidity and whole worth locked (TVL) amongst all layer-2 networks. Coinbase Layer-2 Base has additionally demonstrated vital transaction volume and a rising consumer base, positioning it as a pretty possibility for decentralized social applications.

[deploypolygonzkrolupstuff]

Unlocking Layer 2 Benefits: Why DeFi Projects Should Consider Polygon CDK Deployment

Decentralized Finance (DeFi) has revolutionized the traditional financial landscape by offering open and permissionless access to financial services powered by blockchain technology. However, the rapid growth of the DeFi ecosystem has exposed scalability issues and high transaction costs on Ethereum, where many DeFi projects are built. In response to these challenges, Layer 2 scaling solutions like Polygon Contract Development Kit (CDK) have emerged, offering a compelling case for DeFi projects to consider deployment on the Polygon network.

The DeFi Dilemma: Scalability and Cost

DeFi platforms have gained popularity for their ability to provide a wide range of financial services, including lending, borrowing, decentralized exchanges, yield farming, and more, without the need for traditional intermediaries. However, the success of DeFi has also brought to light some significant issues:

Scalability: Ethereum, the primary blockchain for most DeFi projects, faces scalability limitations due to its consensus mechanism, Proof of Work (PoW). This results in slow transaction confirmations and network congestion during periods of high demand.

High Transaction Costs: Ethereum's high gas fees, particularly during times of network congestion, make DeFi transactions expensive for users. These costs can limit the accessibility of DeFi to a broader audience.

User Experience: Slow confirmation times and high fees can lead to a poor user experience, discouraging users from actively participating in DeFi activities.

The Role of Polygon CDK

Polygon, formerly known as Matic Network, is a Layer 2 scaling solution for Ethereum that addresses these challenges. Polygon CDK is an essential component of the Polygon ecosystem, providing tools, libraries, and templates to streamline the development and deployment of smart contracts and decentralized applications (dApps) on the Polygon network.

Here's why DeFi projects should consider Polygon CDK deployment:

1. Enhanced Scalability

Polygon's Layer 2 solutions significantly enhance the scalability of the Ethereum network. With Polygon CDK, DeFi projects can process a higher volume of transactions, leading to faster confirmation times. This scalability ensures that DeFi platforms can accommodate a growing user base without suffering from network congestion.

2. Lower Transaction Costs

One of the most compelling benefits of deploying on Polygon CDK is the substantial reduction in transaction costs. Polygon's Layer 2 solutions enable DeFi users to interact with applications with minimal gas fees, making DeFi activities more cost-effective. This reduction in fees opens up opportunities for microtransactions, yield farming, and other activities that may have been financially impractical on Ethereum's mainnet.

3. Interoperability

DeFi projects on Ethereum can seamlessly migrate to Polygon using the CDK. The interoperability between Ethereum and Polygon ensures a smooth transition without requiring extensive code modifications. This means that existing DeFi protocols can enjoy the benefits of Polygon's Layer 2 solutions without the need for a complete rewrite.

4. Security and Reliability

While optimizing for scalability and cost-effectiveness, Polygon CDK maintains the security and reliability of the Ethereum network. DeFi projects can trust that their smart contracts and user funds remain secure and tamper-proof, aligning with the principles of blockchain technology.

Real-World Use Cases

Several DeFi projects have already leveraged Polygon CDK to enhance their operations:

Aavegotchi: A popular DeFi and NFT project, Aavegotchi migrated to Polygon to offer users a seamless gaming and DeFi experience with fast transactions and low fees.

QuickSwap: A decentralized exchange (DEX) built on Polygon CDK, QuickSwap has gained popularity as a cost-effective alternative to Ethereum-based DEXs.

SushiSwap: SushiSwap, a decentralized exchange and DeFi platform, expanded to Polygon to provide users with lower fees and faster transactions while benefiting from Ethereum's security.

Conclusion

DeFi projects face a pivotal decision regarding their scalability and cost-efficiency challenges. Deploying on Polygon CDK can unlock a world of benefits, enhancing scalability, lowering transaction costs, and improving the overall user experience. With DeFi continuing to disrupt traditional finance, scalability and cost-effectiveness will play an increasingly vital role in the industry's growth.

As DeFi projects look toward the future, the deployment of Polygon CDK presents a compelling opportunity to optimize their operations and contribute to the ongoing transformation of the financial industry. By embracing Layer 2 solutions like Polygon, DeFi projects can provide users with a seamless and cost-effective alternative to traditional financial services, ultimately fostering broader adoption and innovation in the DeFi space.

[deltainfoteklive]

Angular Material Design: Creating Stunning UIs with Ease

Angular Material Design is a powerful and flexible framework that enables developers to build visually appealing and responsive user interfaces effortlessly. By combining the power of Angular and the elegant design principles of Material Design, developers can create web applications that not only look stunning but also offer an excellent user experience. In this article, we will explore the ins and outs of Angular Material Design and how it can revolutionize your UI development process. Let's dive in! What is Angular Material Design? Angular Material Design is an implementation of Google's Material Design guidelines for Angular applications. It provides a collection of UI components, directives, and services that adhere to the Material Design specifications, ensuring consistency and aesthetic appeal across different platforms and devices. With Angular Material, developers can create interactive and visually engaging UIs without the need for extensive CSS and JavaScript coding. The Advantages of Angular Material Design Angular Material Design offers a myriad of benefits for developers and users alike. Some of the key advantages include: - Easy Implementation: Angular Material's pre-built components can be seamlessly integrated into your Angular project, reducing development time and effort significantly. - Responsive Design: The framework automatically handles responsiveness, ensuring that your UI looks stunning on various screen sizes and devices. - Customizability: Despite providing pre-designed components, Angular Material allows customization to match your application's unique branding and style. - Cross-Browser Compatibility: Angular Material is designed to work flawlessly on popular web browsers, ensuring a consistent experience for all users. - Accessibility: The framework prioritizes accessibility, making your application usable by individuals with disabilities. - Rich Set of Components: Angular Material offers an extensive library of components, including buttons, cards, menus, dialog boxes, and more, simplifying the creation of complex UIs. - Animations: The framework comes with built-in animations that add fluidity and interactivity to your application. Getting Started with Angular Material Design To leverage the power of Angular Material Design, you need to follow a few simple steps: Step 1: Install Angular Material To begin, install Angular Material by running the following command in your Angular project: npm install @angular/material @angular/cdk @angular/animations Step 2: Import Angular Material Modules In your app.module.ts file, import the necessary Angular Material modules: import { BrowserAnimationsModule } from '@angular/platform-browser/animations'; import { MatToolbarModule } from '@angular/material/toolbar'; import { MatButtonModule } from '@angular/material/button'; // Add more modules as per your requirements Step 3: Include Material Icons To use the Material icons in your application, add the following link to your index.html file: Step 4: Add Material Components to Your App Now you can use Angular Material components in your application's templates: My Angular Material App Click Me Styling Angular Material Components While Angular Material provides an attractive default style, you can customize the appearance of components to match your application's design language. To apply custom styles, follow these steps: Step 1: Create a Custom Theme Create a new file named theme.scss in your project's src/ directory and define your custom styles: @import '~@angular/material/theming'; @include mat-core(); $primary-color: mat-palette($mat-blue, 700); $accent-color: mat-palette($mat-yellow, 500); $theme: mat-light-theme( $primary-color, $accent-color ); @include angular-material-theme($theme); Step 2: Include the Custom Theme Add the following line to your styles.scss file to include the custom theme: @import 'theme'; Now, your Angular Material components will reflect the custom styles you defined. Creating Stunning UIs with Angular Material Angular Material offers a wide range of components that can be combined to create visually stunning user interfaces. Let's explore some of the key components and how they can be used to enhance your application's UI: Angular Material Buttons Buttons are an essential part of any UI. Angular Material provides a variety of button styles that can be easily integrated into your application: Click Me Cancel Delete Angular Material Cards Cards are a versatile way to display content in a structured manner. With Angular Material, creating cards is a breeze: Card Title Card Subtitle This is the card's content. You can add text, images, and other elements here. Like Share Angular Material Dialogs Dialog boxes are useful for displaying critical information or receiving user input. Angular Material simplifies the creation of dialogs: import { MatDialog } from '@angular/material/dialog'; import { DialogComponent } from './dialog/dialog.component'; @Component({...}) export class MyComponent { constructor(public dialog: MatDialog) {} openDialog(): void { const dialogRef = this.dialog.open(DialogComponent, { width: '250px', data: { name: 'John Doe' } }); dialogRef.afterClosed().subscribe(result => { console.log('The dialog was closed'); }); } } Angular Material Menu Menus provide a clean and organized way to display options to users. Angular Material offers different types of menus: Menu Option 1 Option 2 Option 3 FAQs Q: Is Angular Material Design free to use?A: Yes, Angular Material Design is an open-source project released under the MIT license, making it free for commercial and personal use. Q: Can I use Angular Material with other front-end frameworks?A: Angular Material is designed specifically for Angular applications. While some components may work with other frameworks, the best experience is achieved within Angular projects. Q: Does Angular Material support theming?A: Yes, Angular Material offers theming support, allowing developers to create custom styles and apply them to the components. Q: Is Angular Material compatible with AngularJS?A: No, Angular Material is designed for Angular (2 and above) and is not compatible with AngularJS (Angular 1.x). Q: How can I contribute to the Angular Material project?A: You can contribute to Angular Material by reporting issues, submitting pull requests, or helping with documentation and community support. Q: Are there any performance concerns when using Angular Material?A: Angular Material is well-optimized for performance. However, like any framework, it's essential to use components judiciously to avoid unnecessary overhead. Conclusion Angular Material Design empowers developers to create stunning and responsive user interfaces without sacrificing functionality. By using the pre-built components and theming capabilities, you can craft UIs that align with your application's branding and offer an excellent user experience. So, whether you're a seasoned Angular developer or just getting started, Angular Material is a game-changer for building visually appealing and functional web applications. Read the full article

[leedsomics]

Unbiased phosphoproteomics analysis unveils modulation of insulin signaling by extramitotic CDK1 kinase activity in human myotubes

Sensitivity and plasticity of insulin signaling and glucose uptake in skeletal muscle depends on determinants such as genetic variation and obesity. We collected muscle biopsies and isolated myoblasts from a multi-ethnic cohort of lean South Asians (N=10), lean Chinese (N=10), and obese Chinese (N=10), and analysed the proteome and phosphoproteome dynamics in terminally differentiated myotubes after a low-dose insulin stimulation (10nM at 0, 5, 30 min). The myotubes initially responded with increased abundance and phosphorylation level changes along the PI3K/AKT/mTOR axis, decreased abundance of translation initiation factors, and increased phosphorylation levels on proteins involved in mRNA processing at 5 min. After the acute response, protein abundance returned to baseline at 30 min, while phosphorylation changes persisted in proteins including AKT, RPS6 and AS160 (TBC1D4). A joint kinase-substrate statistical analysis revealed that protein abundance changes of AKT, PAK1 and CDK1 showed concordant phosphorylation changes in their respective substrates upon insulin stimulation. We also observed increased phosphorylation of some substrates uniquely in each group, particularly the substrates of CDKs showing stronger changes in South Asians than in Chinese. Pharmacological inhibition and siRNA knockdown of CDK1, a non-myogenic kinase, in terminally differentiated myotubes reduced glucose uptake and desensitized several phosphorylation-mediated signaling on protein translation initiation factors, IRS1, and AS160. Our data suggest that basal extramitotic activity of CDK1 is required for PI3K/AKT/mTORC1 signaling cascade and glucose uptake in insulin-stimulated myotubes. The data also provide a rich resource for studying the role of other kinases in the mechanism of insulin resistance in human myotubes. http://dlvr.it/SrYMvM

[aealzx]

Hello there, I just wanted to say that I really love your story and Im so hyped iwanted to make some fanart for it. I was wondering if that is ok with you?

ohmgosh you caught me off guard with this

of course you can make fanart! do you need refs? I can scribble up some refs if you need

THANK YOU!

[aitoolswhitehattoolbox]

Java Lead

COUNTRIES OVER 1,400 ACTIVE GLOBAL CLIENTS Position: Java Lead Location: Remote (U.S) Duration: Fulltime… Job Responsibilities Must have : JavaScript, Java/Spring Boot, Chatbot development, Kubernetes, Docker, AWS Lambda, AWS LEX, AWS CDK… Apply Now

[incorrectacmilanquotes]

Pioli: *referring to CDK* Apparently Paolo is getting us another kid.

Mike: Are we stealing him?

Simon: New or used?

Pioli: Wonderful responses, both of you.

[rojinfo]

Attentat terroriste contre le siège du CDK-F à Paris: Appel à une grande mobilisation samedi 24 décembre

Attentat terroriste contre le siège du CDK-F à Paris: Appel à une grande mobilisation samedi 24 décembre

Un attentat terroriste contre le siège du CDK-F à Paris, ce vendredi, en fin de matinée, a fait au moins trois morts, dont la responsable du Mouvement des Femmes kurdes et un chanteur kurde. Le CDK-F appelle à une grande mobilisation samedi 24 décembre, à 12h, sur la Place de la République. L’attentat terroriste perpétré contre le siège du Conseil démocratique kurde en France (CDK-F), dans le…

[science-lover33]

DNA Replication

Consists of creating an exact replicate of itself; each strand serves as a template for synthesizing a complementary strand; this replication process is semi-conservative. The new DNA has formed thanks to enzymes called DNA polymerases; this needs a primer synthesizing DNA from the 5' to 3' end. This process also includes other enzymes such as DNA ligase, DNA primase, DNA helicase, and topoisomerase, "this process takes us from one starting molecule to two "daughter" molecules, with each newly formed double helix containing one new and one old strand" (Clark, Douglas, Choi, 2018).

Let's talk about the differences between DNA replication in eukaryotes and prokaryotes. We could start by saying that prokaryotic organisms have circular chromosomes with a less extensive coiling structure than eukaryotic chromosomes. The replication process begins with a specific sequence of nucleotides called the origin of replication which has approximately 245 base pairs rich in AT sequences; the helicase is responsible for breaking the double helix in two and unwind the hydrogen bonds between the hydrogen base pairs, the ATP hydrolyzing process is necessary because requires energy.

This process requires three essential types of enzymes, DNA polymerase I, which acts later in the process. DNA polymerase II is used to repair, and DNA polymerase III acts by providing new nucleotides one by one doing it in the 3' to 5' direction. "Topoisomerase binds at the region ahead of the replication fork to prevent supercoiling or over-winding" (Clark, Douglas, Choi, 2018).

On the other hand, we have replication in eukaryotes which although it starts in a similar way to prokaryotes, the process in eukaryotes has a pre-replication phase which contains proteins that initiate the process, "the leading strand is continuously synthesized by the enzyme pol δ, the lagging strand is synthesized by pol ε" (Clark, Douglas, Choi, 2018). Here the genome is much larger than in prokaryotes, so the replication process is slower, making a ratio of 50 to 100 nucleotides per second; DNA polymerases have the names pol α, pol β, pol γ, pol δ, and pol ε.

Their DNA is packaged when chromatin fibers begin to condense around proteins called histones, which condense to form linear chromosomes. The end of which are called telomeres which are non-coding DNA sequences for a particular gene and protect the information from being deleted as the cell divides. A highly regulated DNA replication process is found in eukaryotes and almost always involves extracellular signals to coordinate specialized cell divisions in different tissues of multicellular organisms. Cyclins are synthesized during the G1 process of the cell cycle, and external signals trigger their synthesis. To facilitate the synthesis of the S-phase proteins, DNA polymerases, and thymidylate synthesize, cyclins form complexes with cyclin-dependent kinases (CDK). The cells are primed for DNA replication during the S process by these complexes(Tabancay & Forsburg, 2006).

"In the final stage of DNA replication, the enzyme ligase joins the sugar-phosphate backbones at each nick site. After ligase has connected all nicks, the new strand is one long continuous DNA strand, and the daughter DNA molecule is complete" (DNA Replication in Eukaryotes, 2020). The environment in which they live; prokaryotes are not formed by cells, so they do not need complex machinery to function, however eukaryotes that are usually multicellular need a more complicated process because their requirements are higher, this is why more enzymes and fewer nucleotides are involved in eukaryotes and fewer enzymes and more nucleotides per second in each case.

References

Clark, M. A., Douglas, M., & Choi, J. (2018). Biology 2e. OpenStax.

DNA Replication in Eukaryotes. (2020, August 15). Retrieved March 2, 2021, from

https://chem.libretexts.org/@go/page/13293

OpenStax, Concepts of Biology. OpenStax CNX. May 18, 2016

http://cnx.org/contents/s8Hh0oOc@9.10:2ousESf0@5/DNA-Replication

Rye, C., Wise, R., Jurukovski, V., DeSaix, J., Choi, J., &; Av, Y. (2016). Biology. Houston, TX: Openstax.

Tabancay, A. P., & Forsburg, S. L. (2006). Eukaryotic dna replication in a chromatin context. Current Topics

in Developmental Biology, 129-184. doi:10.1016/s0070-2153(06)76005-7