#giemsa

[gonyadaldysgenesis]

sex chromosome mosaicism flag

[pt: sex chromosome mosaicism flag]

chromosomal mosaicism is a phenomenon which describes someone who has more than one makeup in the number or orientation of their chromosomes for any given pair. sex chromosomal mosaicism only affects the 23rd pair in humans. the most common types of sex chromosome mosaicism that we know of are XO/XX, XXY/XY, and XX/XY, but many different combinations are possible.

the flags background was chosen from the intersex flag. the inner circle is made of a "mosaic" of colors from common stains used to create karyotypes, primarily giemsa dye, which is primarily indigo, blue, and purple, with hints of magenta.

[nethhiri]

Marooned: Chapter 23

Kid x FemReader x Killer

Warnings: Murder, violence, torture, gore, sex

Blood & (Rearranging) Guts

There was a giddy atmosphere on the Victoria Punk. The crew was bustling around, pushing canons, loading guns, sharpening blades. Your weapon, of course, was already sharp and loaded, but you wanted something else, which is why you were rummaging around in the kitchen. You hoped Killer wouldn't mind if you borrowed some things. It wouldn't take long to catch up to the Marine ship, especially at the pace you were moving. Kid ordered the Punk to sail at full speed once they were sure they were pursuing the right ship.

Back on deck, Minerva found you and followed you to where Killer and Kid were standing. Whatever their conversation was, it stopped when you walked over.

"Don't stop on my account." You leaned against the railing, looking towards the bow, which would be approaching the other ship any minute. 

"Well, princess, we were just sayin how ya shouldn't expect any of us to watch yer back," Kid started. "Since ya like to remind us that yer not a part of this crew so much."

Your eyes rolled at the pet name. You were the precise opposite of a princess. "Don't expect me to watch yours either, Captain," You mocked. "Try to stay out of my way, would you?" You patted his back condescendingly as you walked past him, headed towards the bow. You wanted to have the first opportunity to board. 

Killer caught your shoulder, leaning down to whisper in your ear. "Those better not be from my kitchen, darlin." His low voice vibrated against your ear.

"Nothing gets by you, huh?" You motioned for him to bend down again so you could whisper back, "I'm sure I can make it up to you somehow." You winked at him and continued to the bow.

You sat on Mini's back, waiting for your chance to board. The ships were close enough to exchange canon fire. It seemed like Eustass was having fun flinging their cannonballs back at them. You made a mental map of the path you needed to take to get to Giemsa the fastest. You figured Kid would be distracted by killing the marine grunts, but you didn't trust him not to kill your prey if he found him first. 

Finally, the marine ship was close enough. Your fingers tightened in Mini's fur as she barreled towards them, using the railing as a kicking off point for an extra boost. To say the men on board were shocked to see an enormous hog land on the ship was an understatement. Using their moment of shock, you tore through them to get to the stairwell. You didn't even have to ask Mini to cover you. That was a given.

Everything else was tuned out the moment you put your hand on the handle of your gunblade. It was slower getting up the stairs, since you had the low ground. Normally, a captain should be fighting with their men, but you knew Giemsa was a coward at heart, only fighting people he could overpower. You were saving your bullets, however, the task of getting up the stairs was slower than you liked, and you were an impatient woman. Luckily, the stairs were narrow, which lended itself to lining the men up nicely for you. You plunged your knife into the belly of the man in front of you until the barrels of the gun portion of your weapon were flush against him. The line of men in front of you fell to their knees as you pulled the trigger. Infusing your weapon with haki let you shoot through the lot of them. Now, it was a semi-clear path, you just had to climb over some bodies or push some over the railing. You heard the wails of them behind you as Mini also stepped over them, a few hundred pounds heavier than yourself. You really didn't care if you killed them or not. They would die eventually and they would die suffering. Maybe they didn't even know about what their captain was up to. That didn't matter to you either. Guilt by association. Your moral compass had broken along with your spirit back then.

The closer you got to Giemsa's office, the more your heart raced. Not with anxiety or fear, but excitement. Just thinking about your plans for him brought a smile to your face. That smile was incredibly unsettling to the marines that encountered you. The ones who fought you were unnerved and some were frightened away altogether by what they saw. You let them go. The Kid Pirates were behind you after all. You wondered which enemy was more terrifying to them. 

Mini had a blast barreling through the throngs of marines that were trying to defend their captain. Her coarse fur and thick skin prevented their blades from doing much harm, even more so because the wielders were pathetically weak. The boar would spear through her adversaries with her tusks and shake her head back and forth to fling them off. And if she didn't kill them like that, she trampled them. Clearly, she had a lot of pent up energy from being stuck on a boat, unable to run around. 

Walking through the now-clear hall, you stood in front of the door you knew led to Giemsa. Instead of kicking it open, you knocked in code. It was something marines did sometimes to identify each other if they didn't know who was on the other side of the door. Thinking you were another marine, you were told to enter. He was facing away from you, hurriedly explaining the situation on a Den Den Mushi. "M-mayday! T-there's Kid Pirates everywhere. They-" He was cut off as you shot the snail from his hand. Good. He thinks it's only Kid Pirates. He did until he turned around. Then, whatever state of despair he was in was multiplied tenfold as his eyes went wide.

"Didn't I tell you I would come back?" You sat on his desk, across from him, gun pointed at his head. "Lose your weapons, pretty please." He did as he was told, pulling his sword from its scabbard and throwing it down. "Good boy, now strip." He gave you a pathetic look. "I don't trust you to actually disarm yourself, so strip." You used your gun to point to where you wanted him to stand. "And bring your chair over here."

He slowly started to disrobe. "Y-you know there's other marines on their way. A-admirals maybe even."

You put a warning shot through the wall next to his head. "Shut up and strip. No one is coming for your bitch ass." Where the fuck are you, Kid? I'm not waiting. You bit your lip. Maybe you could just do a tiny bit of torture and then heal him before Kid comes so he's a fresh canvas. You tied him to the chair with his own clothes, save for the jacket, which you were now cutting your trophy from. As you shoved it in your pocket for later, there was a loud commotion from outside. There he is. Giemsa's eyes shifted between you and Kid as the red-headed captain entered. As much as you didn't want to share this moment, you delighted in seeing Giemsa's hope of surviving this evaporate before your eyes. 

"Took you long enough."

"I was cleaning up yer mess! Ya left a bunch of them alive!" 

"They were gonna die eventually."

"Don't ya like to watch em die though?"

"You guys are psychopaths!" Giemsa couldn't stop himself from blubbering out loud. It wasn't the smartest move, drawing the attention of both of you.

You took a small paring knife out of your belt. Kid laughed behind you. "So THAT's why Killer was in a huff this morning."

"I'm borrowing it." 

Kid's curiosity was thoroughly piqued. Usually he went for the big knives if he wanted to torture someone. "Show me what ya got, Sea Snake."

You lifted Giemsa's chin in your hand. "Since you enjoyed so much what was done to my face, I'll grant you the honor of matching." 

Before you started, you tore a few strips of fabric from his discarded clothes. With some, you gagged him, and with the others, you wrapped them around your left hand. With the small knife, you started to trace a rough outline of your scars, replicated on his face. He whimpered. "Oh hush. I haven't even started. Just wait until I get to your eye." You took the little knife, starting from his head, and went over the small lines you had made, cutting until you could see the silvery sheen of fascia. Gripping the flap of skin with your fabric-wrapped hand, you started puling and cutting at the same time, no easy task when your materials were struggling beneath your hands. The fabric in your hand helped you keep your grip on the otherwise slick with blood flesh.

A hand came from the side of you and tightly gripped Giemsa by the hair so that he couldn't move his head. You gave a questioning look to Kid. "What?! The faster yer done, the faster I get a turn." Kid watched intently as you skinned half the man's head. No question you had practice with it. There was a finesse to your work. Even after blood was dripping down your elbows, you never lost your grip. Kid almost forgot to revel in the man's torment because he was so lost in the way your hands worked, and how good they looked glistening in red-brown hues.

It was satisfying to watch the skin peel away. Yes, you were aware that it was pretty fucked up to be enjoying this. No, you didn't care. He had it coming. Briefly, as you held up the skin you had cut away, you wondered if you could mail it to Marine Headquarters, similar to what the Warlord, Trafalgar Law, had done. You smirked at your handiwork. Just as your face had been marred, so too, was his. Flinging the skin towards where Mini was waiting, you went back to the task at hand.

Your hand squeezed over Kid's, making sure his grasp was firm. "Hold him tight. This is the best part."

Kid felt his skin heat up where you had left a bloody handprint. The way you bit your lip with concentration and how your voice was practically a purr when you gave him instruction made his heart race. 

You grabbed the side of Giemsa's head that was missing skin, causing an intense stinging pain to him, though you barely heard his shrieking through your intense focus. Sliding your thumb up, you dipped it towards his eye. "I really wish you could live and learn what it feels like to have your vision taken from you, but sadly, you do have to die." Pushing your thumb under his eye, it was warm and wet. You maneuvered your hand to loosen the muscles holding the eye in place. During this, Giemsa screamed so hard you thought he passed out for a minute. Finally, his eye popped out, hanging on by the nerve. Wrapping it around your finger, you yanked on it. You held it up in front of his other eye so he could see it, IF he could see it, given that blood and tears were running down his face. "Mini." The boar had been patiently waiting in the background, Killer had been, too, you noticed. She opened her mouth for you to toss your prize into. 

Wiping your hands on your clothes, you let out a sigh. Even though it should have been the opposite, for a normal person, you felt a weight lift from you. This was a catharsis. "Your turn, Captain." You walked past him towards Killer. "Try to impress me." Kid growled at your remark and immediately went to work on Giemsa, choosing blunt force as his tool.

It was hard to understand the flayed man, though you could make out him asking why Kid was now his torturer.

"Cuz ya fucked with me and my crew." Kid had the man encased in various metal pieces, crushing different parts of him as he pleased. "And based on what I've heard of ya, I can only guess what yer plans were with my girls." You could hear bones break from across the room. 

Killer had his arms folded, watching the little show in front of him. "Nice technique." 

You were sharpening the paring knife with a small whet stone, also borrowed from the kitchen. "Thanks," you grinned.

The blonde was definitely displeased with your use of kitchen tools. "You could have asked if we had any knives specifically for this."

"Oh yeah, Killer. Let me just ask for your finest torturing instruments." As if that was the weirdest thing that you could have done at this point. "You barely let me have my own weapon. AND I know the kitchen knives are way sharper."

"Not anymore." His voice was softer.

"Oh my god. Are you pouting?" It was in his tone. Cute.

"Don't push it. I didn't have to let you take them." Killer tilted his head. "I was curious about what you were gonna do."

"I told you I would make it up to you." 

"You will." 

His deep voice sent a shiver up your spine and you weren't mad at his threat. A particularly loud scream drew your attention back to Kid. "Don't kill him! That honor is mine, remember?" You got closer to see what he was doing. You could tell he overdid it. There were shards of metal sticking out of Giemsa everywhere and he was nearly unrecognizable with swelling and bruising. "Come on, Kid!" You pushed him exasperatedly. "He's practically dead!" 

"But he's not!" Kid wiped his brow, getting rid of sweat, yet smearing deep red across it. "I held my end of the deal up."

"Barely." 

"So-rry I don't have fancy knife skills like ya." He feigned an apology and snickered. 

"Okay." You said, nodding irritatedly. "Then you're gonna learn." You handed him the kitchen knife, handle first.

Kid's eyebrows knit together. "Hah??"

You thrust the knife at him again. "Take it." He took it, an unsure look in his eyes. "Okay, now take the other half of his face off. You were watching how I did it." You folded your arms. Kid looked at you again and started to emulate what you had done. Killer had walked up, too, out of curiosity, to see how Kid would fare. 

Kid cursed as the knife created a buttonhole in the skin he had tried to peel off. After a few more times, he threw his hands up. The man had little patience. "Are you happy? I tried." 

"Uh-uh," you tutted. "You didn't want to stop before and you're not stopping now."A wry smile crept onto your face. "If I can't finish torturing him how I want, then you'll do." Before Kid was able to protest, you put your hand over his that was still gripping the knife, comically small in his huge hand. His arm was a lot longer than yours, putting you in a somewhat awkward position, pressed against his side. It was hard to ignore the smell of iron and sweat that radiated from him, not unpleasantly so. You tilted his hand slightly, "Your angle is wrong." The knife glided more smoothly, but it still snagged and punctured through the skin. Your usual admonishing tone was replaced with an encouraging one, "Too much force. Lighten up." You could feel Kid was tense under you. Your hand slid to his wrist and shook it until he relaxed. "That's better." You let his hand go, resting it on his shoulder next to your other hand. Once you saw he had the hang of it, you let him finish the job. Somewhat regretfully, you let go off his shoulder and stood back. 

Kid was annoyed at first, you bossing him around. He only tried it initially to stop you from bitching at him. So what if he almost killed the guy, you wanted him dead anyway, and Kid wasn't used to sharing. When you put your hand over his, though, his thoughts were instantly clouded. Even more so, when he felt the rest of your body pressed up against his. He didn't even hear what you were saying, more focused on how your words tickled his neck than anything else. Kid was only shaken back to reality when you grabbed his wrist to get him to relax. He felt you lean on his shoulder, resting your chin on it while you were watching his progress. It was either the adrenaline or the testosterone pumping through his veins, maybe it was just you, covered in blood, with your sweet lips so close to his ear and your nails digging into his skin with every stroke of the knife, like you were enjoying it; either way he wanted to sling you over his shoulder and throw you on the bed in his cabin. Now, he watched you. After he had finished, it was your turn again, even though the poor excuse of a man was hanging onto life by a thread. Kid's eyes followed your grinning face as you took out your stupid gun/knife out and pressed it to the man's throat. A part of him wished he was the one under your burning gaze, maybe with a knife, maybe sitting on top of him. Slowly, you dragged the blade over Giemsa's throat. Instead of the red spray that was typical, there was a sad, red dripping line. The look you gave to Kid was withering as you stood in front of him, yelling something about how the guy's blood pressure was so low it wasn't even satisfying. His usual instinct was to yell something back at you, yet all he wanted to do was tangle his hand in your hair and devour your blood-stained body. 

"Aw y'all look so precious torturing together," Killer was smirking under his mask, you could tell. 

You didn't have a chance to voice your rebuttal before Kid bounced a bolt off his first mate's mask. "SHUT UP, KILLER."

Killer couldn't resist. It was an easy taunt. And judging from the way both you and Kid went red, well, it was hard to tell since both of your faces were spattered in blood, Killer was sure he would do it again. Kid was so cute when he got flustered. Killer couldn't blame him though; you were something else. Killer tried not to indulge himself in thoughts of you too often. He knew you would be a fleeting thing aboard the Victoria and he preferred to take his time getting to know someone. Pretty much the opposite of Kid, who liked to get to know someone starting at whatever hole he could get to first.

It was dusk when the three of you emerged on the deck. You hadn't realized how long you had been in there for. There wasn't a soul in white remaining on the marine ship. The only living occupants were the Kid Pirates looting it for maps, gold, and food. I'm hungry. 

As if reading your mind, Killer asked his captain, "So are we going to feast tonight or what?" He was watching several crew carry kegs filled with some kind of alcohol, and crates of food fresh from the island you had just left. 

"Fuck yeah," Kid replied enthusiastically. 

As hungry as you were, you were not about to eat without washing the gore off. And you had some things you needed to get out of your mind, especially before drinking, namely Kid's scent and the way you could feel his muscles move under you. Both of those things were easier said than done. The first problem was every other girl had the same thinking as you, so all the showers were full. The second problem was that Kid was very hot and it was hard to banish certain thoughts you had about him, chiefly since you couldn't find a goddamn shower to quietly relieve that tension. 

You paced the hallway, waiting for someone to finish up. "Other people have to shower too, you know!" You banged on the door. 

"I know where there's a shower." You turned to see Kid smugly leaning against his doorframe.

You looked him up and down. "Not a chance." You couldn't take the bait that easily. You would never live it down.

He shrugged. "Suit yerself." Kid vanished into his cabin. He knew you would be back. After all, he had used his powers to turn on the showers and lock the door of the women's bathrooms. They had been long finished by now. 

You paced for another 20 minutes before giving up. Fuck it. Sighing, you knocked on Kid's door. It opened almost immediately, like he'd been expecting you. "Can I use your shower?" You mumbled.

He put his hand to his ear, like he was waiting for you to say something else.

"Can I use your shower please, Captain?"

"Only because I'm so nice," He held the door open for you. "Ya know where it is."

You expected to be jumped, but Kid was busy looking at something on his desk, maybe one of the maps they had stolen. Your brows furrowed. You shrugged your jacket to the floor, sneaking a peek over your shoulder. Nothing. You opened the door to the bathroom but didn't close it, undoing your belt and kicking your pants off, as you leaned over to turn the water on. You could see in the reflection of the metal faucet that Kid still wasn't looking. It was making you a little mad. You didn't necessarily want him to be all over you, though now you were a bit offended that he wasn't. What? I'm not hot anymore? Another thought occurred to you. Did I go too far? He's turned off by what he saw me do? 

Kid knew you were looking at him. He was pretending to look at a map, though he was actually trying to gauge your true interest in him. He was always the one that pursued you and sometimes under very dubious pretenses. But now he was genuinely curious. Did you want him? Of course he enjoyed when you struggled, because he knew you were into it, or you were eventually into it. After everything that transpired, he didn't want to accidentally overstep. It wasn't enjoyable if you weren't also enjoying yourself. 

Now, you were standing in the doorway, stark naked and still nothing. Your eyes narrowed. Okay. I'll get his attention. One well-aimed shot did the trick. 

Kid's head snapped up, finally looking at you. "WOMAN, ARE YA INSANE?" That was so close he could feel the heat from the bullet. 

"You didn't even peek at me!" 

Kid slammed his fist on the desk. "Gods, ya know I wanted to! I thought if I ignored ya, ya might put yer titties in my face or somethin! Not shoot at me!" 

You shrugged. Yeah that's probably what a normal woman would do. "I guess." You walked over and stood in front of his desk, hands clasped behind your back. 

"Don't ya fuckin act shy now! Ya shot at me and tried to seduce me."

A smirk tugged at the corner of your mouth. "I thought maybe..." You bit your lip. "I thought maybe I went a little... overboard earlier... and scared you off." 

Kid threw his head back and laughed. "Are ya fuckin kiddin me? I'm Captain fucking Kid! It was the hottest thing I've ever seen." Kid stood up and started to undo his belts.

"No. Stop." You walked around the side of his desk and gently pushed him to sit back down. "Let me." You slid down between his knees, a little too eager to get past the obstacles between you and his dick. He helped you tug his pants down far enough to let his cock spring free, sucking in a sharp breath when you spit on it and took it in your hand. You knew you would gag if you tried to take the entire thing so you focused on the tip and used your hand for the rest.

Killer chose that moment to pop his head in, since the door was wide open. All he could see was Kid at his desk. "Hey, are you gonna get in?" He said thumb over his shoulder pointing to the shower. "Water's been on for a while." 

Kid made some intelligible grunting noise.

Killer couldn't tell if that was a yes or no. "What?" He looked down at the floor, littered with your clothes, and back to Kid, who had his head tilted back. Killer was fairly familiar with that face. "You're gettin sucked off, aren't you?" He sighed. "Okay, well I'm gonna get in then." He started to close the door, mumbling about wasting hot water.

You heard Killer in the doorway ask Kid something, as you bobbed your head up and down. Remembering your promise to make it up to him for stealing his kitchen knives, you poked your head up. "You wanna join?" 

Killer faltered for a moment before slamming the door, making you snicker. 

Kid pushed your head back down. "Girlie, you're mine fer tonight. I ain't sharin," he growled, gripping your hair and making you go faster.

Your eyes were tearing up as his cock nearly made you choke every time he pushed your head down. Suddenly, he pulled you off him and up, pulling his legs closer together so you could comfortably sit on top of him. Kid was very obviously impatient as he grabbed your hips and pushed you down on his cock, filling you up in one swift motion. You buried your head into his head and moaned. His grip prevented you from grinding on him, which you desperately wanted to do, instead bouncing you up and down on him. With each downward motion, he was hitting your cervix. It was painful, but it was a good pain. You felt his tongue slide up the side of your neck, him moaning as he tasted the remaining blood and  biting at your ear. You yelped but returned the favor, alternating biting at his neck and licking at the blood that was dried on it, from his shoulder to behind his ear. "You're disgusting," you breathily whispered. "I like that about you." Kid laughed between panting and lifted you up, slamming your back against the desk, him hulking over you. As you cried out, he bent down and shoved his tongue in your mouth. Both of you sucking at each other's tongues and biting at each other's lips. Your hands found his hair and tugged him closer to you. He swallowed every moan you gave up for him. The way your thighs gripped him tighter and tighter gave away how close you were. 

"Hate me so much but yer little pussy can't get enough of me can it?" Kid teased. "Say it." Kid pressed his thumb into your clit.

"I h-hate you." You played dumb on purpose, defiantly smirking up at him. 

Kid growled and closed his metal and on your throat. "No, the other thing," he hissed through clenched teeth, clearly trying to hold out. 

"T-this..Nnnlittle," you couldn't think straight between his thumb rolling across your clit and the deep strokes he was pounding into you. "Fuck!" Your back involuntarily arched and heat filled your body, sending waves of shaking pleasure down to your curling toes. 

The rhythmic clenching of your sopping wet pussy drove Kid over the edge. He wanted to stay buried in it, but he pulled out and sent ropes of hot cum to splatter across your body that was so beautifully laid out for him on his desk. The only thing that would be better was if the blood was still wet so he could really make a mess of you and smear it together with his cum. You both stayed like that until you caught your breath. 

It sounded like Killer was done in the shower. He probably showered as fast as possible so he didn't have to hear Kid fucking you. Kid took off the rest of his clothes before slapping your tit and squeezing it. "Get yer ass in the shower. I'm tryna eat that pussy, too." 

He didn't have to tell you twice and soon your back was pressed against the cool side of the shower, both your legs over Kid's shoulders. His tongue and fingers were unrelenting as he ripped another orgasm from you. You doubled over, gripping so tightly at his hair that your fingers were cramping, toes curling so hard that they were cramping, too. "Ah! F-fuck! Fuck!" Partly crying out from pleasure and partly crying out from pain. Kid finally let you down, stabilizing you with his arm when your legs refused to stop shaking.

He taunted you, "Poor brat can't even stand on her own." 

"Shut up," you pushed his arm away. You started washing up, trying to get the dried blood out of your hair. Kid did the same. "Did I miss any spots?" 

Kid looked you over and used his thumb to rub away something from your temple. "What about me?" 

You looked at him with his wet, red hair plastered on his pale forehead. His appearance was so different without his lipstick and goggles. "Just your ugly-ass face." You flicked his crooked nose. 

"Rotten, yer so lucky I'm hungry or I would fuck ya until ya lost yer voice from screamin so loud." Kid squished your cheeks in his hand. "Then ya couldn't run that smart mouth of yers."

You giggled at his threat, turning your head to his thumb and resting your lips on it, playfully taking it in your mouth and coming off it with a 'pop'. "What are you doing after dinner?" Your lust-filled eyes slid up to meet his. 

When you both eventually emerged from below deck, everyone else was already deep into the festivities, and by festivities, you meant booze. Killer sipped through a straw and waved to Kid, leaning back in a chair. Everyone else was horsing around or stuffing their faces. You looked around and saw Heat beckoning you over. Next to him was Wire. 

"I see you're friendly with the captain again." Heat casually observed. He was doing something with a bunch of bottles half-full of liquid. 

You were surprised to see the marine ship still tethered to theirs. "Yeah, well, we were going over a map." You were certainly doing somethingover a map.

"Does that usually involve biting each other's necks?" Heat saw your glare and held his hands up. "Just askin. I don't know. I'm not a captain or anything."

Wire laughed at that.

"Fuck off, Heat." You laughed, touching your hand to your neck. You could heal them, but you really didn't care who saw or what they thought. Though you knew Quincy, Dive, and Emma would definitely give you a hard time. "Why is this thing still here?"

Heat was now stuffing rags into the tops of the bottles. "Every party needs a bonfire, Y/N." He handed you a few and lit the rags for you.

A grin split your face. "I see." You cocked your arm back and threw one bottle after the other towards the other ship. 

Wire and Heat did the same next to you and a few others joined as well. When the other ship was fully engulfed, the ropes tethering it were cut. You leaned against the railing and watched it burn. The heat from the fire rolled over your face. It felt good. One down. Two to go. You sighed. What would you do now? Leave at the next island? Stick around? No one had asked you to leave or stay. You were dragged out of your thoughts, no, literally dragged, you noticed. There was a purple glow and you felt some kind of metal around your center. Kid. He set you on a bench between himself and Killer. 

"Come sit with us, girlie," Kid slung his arm over your shoulders. "How does revenge feel?"

Killer set a mug down in front of you, complete with a straw like his. You took a long drink. "Feels even better with a lovely bonfire afterwards." The other ship was slowly drifting away. You turned back to Killer, who was taking things from his plate, like chicken bones, or other inedible scraps, and putting the under the table. You glanced under the table, already knowing what you would see, and looked back at him with a warmth in your eyes. Mini was happily munching on a pile of scraps that had been dumped at her feet, evidently placed there by not just Killer, but Heat and Wire, too, based on the cleanliness of their plates. 

Killer scratched behind his neck. "Seems better than tossing it in the garbage." Killer hated wasting food. 

You had wondered why Mini hadn't greeted you when you came on deck. Her heart had been swayed by chicken bones and apple cores. After eating your fill, you sat at the table and listened to Wire and Heat brag about who killed more marines. It was the most you heard Wire say so far. Kid joined the conversation, but instead of bragging about himself, he was telling them what you had done. 

"Ya should have seen it! Maybe the next one ya can show em." Kid elbowed you in the side.

Both Killer and you lent a sideways glance to Kid. Next one? 

Kid laughed. "She used Killer's good kitchen knives, too." He grabbed his friend's shoulder. "Probably only let her get away with it cuz she's got good-" It was your turn to elbow him. Hard.

"You talk too much when you're drunk." You got up from the table and went to find the girls. You wanted to hang out with them. If for no other reason than to avoid hear anything embarrassing come out of Kid's mouth. Just as you thought, they were unrelenting in their teasing when they saw the marks on your neck. "Whatever. At least I'm getting laid." You flipped them off. This started bickering between them when Emma was caught in a lie, claiming that she had, in fact, also gotten aid recently. Then Quincy started bitching about how there weren't many good options on board and she couldn't wait until they docked in another few days so they could hit the bar. You were in a fit of laughter as they started physically fighting, yanking each others' hair. You were all too distracted to notice Kid coming up and hoisting you over his shoulder. 

It was the best night of sleep you had had since the first time you had slept in this bed. You were tangled in sheets and covered in red lipstick. Black sheets were a wise choice when you went to bed in red lipstick all the time, you supposed. Kid was in a deep sleep, snoring the house down. How you slept through that, you would never know. You stretched out and finally noticed the reason you had woken up: Killer was staring at you from the foot of the bed. You sat up quickly. "Uh, a 'good morning' would be less creepy." 

He tossed a white piece of clothing at you. "Kitchen. 10 minutes." It was an apron.

You were still a little tired and sore from the night prior. But this intrigued you. You rushed out of bed, quickly washed up, and threw clothes on. Trying not to appear too eager, you went to the galley. "So am I making it up to you in this?"  You gestured to the white apron. 

Killer nodded. He walked up to you and adjusted the apron slightly. "Close your eyes." 

You did as he asked. Killer was a bit of a mystery to you, but you thought he was probably kinky. You felt his hands at your waist, lightly positioning you in front of the counter. He put something in your hand and curled your fingers around it. It wasn't what you thought it would be. You opened your eyes. In front of you was a cutting board and some vegetables. 

Killer was very amused by the confused expression on your face. "Get chopping, darlin." He started prepping some other things.

"What's this?"

"This is how you're making it up to me, line cook." 

You blinked at him. "Are you serious? I thought..." Your face flushed. The mood soured for you as you realized you would not be getting any morning action. 

Killer laughed. "You thought I was gonna fuck you."

"No!" It was hard to be mad at him when his laugh was so disarming. 

"You did." 

You channeled your frustration into chopping. 

Next Chapter

[midseo]

Safranine 0, Safranine Basic Red 2, Manufacturer, Exporter, India

Safranine 0, Safranine Basic Red 2, Safranines, Manufacturers, Supplier, Exporter, Mumbai, India.

Chemical, Chemicals, Electroplating Chemical, Electroplating Chemicals, Lab Chemical, Lab Chemicals, Laboratory Chemical, Laboratory Chemicals, Industrial Chemical, Industrial Chemicals, Safranine, Safranine O, 7 Diamino 2, 8 Dimethyl 5 Phenyl, Phenazinium Chloride, Diethyl Safranine, N Diethyl Phenosafranine, 3 Amino 7 dimethylamino, Basic Violet 5, Basic Violet 8, Thioflavin T, Basic Yellow, Basic Yellow I, O Cresolphthalein, Thymolphthalein, Biological Stain, Biological Stains, PH Indicator, PH Indicators, Lab Reagent, Lab Reagents, Laboratory Reagent, Laboratory Reagents, Dyes & Chemicals, Dye & Chemical, Dyes & Chemicals, Acid Fuchsin, Alizarin Yellow, Alizarin Yellow GG, Alizarin Yellow R, Aurin, Rosolic Acid, p-Rosolic Acid, Azure A, Azure B, Azure 11, Azure 11 Eosinate, Barium Diphenylamine Sulphonate, Basic Fuchsin, Biebrich Scarlet, Brilliant Green, Bromocresol Purple, Bromocresol Purple Sodium Salt, Bromophenol Blue, Bromophenol Blue Sodium Salt, Bromophenol Red, Bromothymol Blue, Bromothymol Blue Sodium Salt, Calcon, Solochrome Dark Blue, Calconcarboxylic Acid, Calmagite, Carbol Fuchsin, Chromotrope 2B, Chromotrope 2R, O Cresolphthalein Complexone, Cresol Red, Cresol Red Sodium Salt, 2:6 Dichlorophenol Indophenol Sodium, 4',5' Dibromofluorescein, Dimethylglyoxime GR, Eosin Blue, Eosin Yellow, Free Acid Eosin Yellow, Water Soluble Eosin Yellow, Eriochrome, Solochrome Black T, Ethyl Orange, Evans Blue, Fluorescein, Fluorescein Complexone, Calcein, Fluorescein Sodium, Giemsa Stain, Hydroxy Naphthol Blue, Indoine Blue, Leishman Stain, May & Grunwald Stain, Methyl Blue, Aniline Blue W/S, Methylene Violet 3Rax, Methyl Orange, Methyl Red, Methylthymol Blue Complexon, Methyl Violet, Naphthol Green B, Naphtholphthalein, 1 Naphtholphthalein, Neutral Red, Orange G, Nitroso R Salt Gr, Indole 3 Acetic Acid, New Fuchsin, asia, asian, india, indian, mumbai, maharashtra, industrial, industries, thane, navi mumbai, pune, nashik, aurangabad, ratnagiri, nagpur, ahmednagar, akola, amravati, chandrapur, dhule, jalgaon, raigad, sangli, satara, belgaum, kolhapur, belgaon.

[literaturereviewhelp]

For a number of possible variations, there exist other measures that enable safeguarding due to the possibility of defects in the equipment used. There are various categories of tissue stain control agents such as reagent controls that are basically antibodies when tested using control material. Staining procedures during the early years involved the use of readily available reagents such as potassium dichromate, alcohol, and mercury or mercuric chlorides for the purpose of hardening tissues. In some cases, the reagents used included: silver nitrates, Giemsa, and hematoxylin among others. While there exists a range of staining methods, some methods have not been used because the chemicals involved are toxic and can cause damage to tissues if control measures are not applied (Baker et al. 127). This literature review shows that there have been improvements in the histopathology of staining and the current methods used are less toxic and enable attainment of the objectives of the staining process as well as an examination of tissues. There also exist positive tissue controls that involve tissues with particular antigens at a known level of stability. The aim of these controls is to provide documentation of correct tissue staining. There also exist internal tissue controls that involve the existence of target antigen in the normal elements of the tissue being investigated. This paper elaborates on the different types of controls involved in tissue staining and how they are applied in diagnostic tasks in the laboratory. Controls contribute considerably towards the interpretation of the stains, and if they are omitted, risks are involved. While it is not practical to develop and run controls for various factors during the analysis of stains, control information in immunohistochemistry and research is important for each valid outcome of the staining process. When controls are used correctly, they enable understanding of the possibility that the stain protocols were run in a correct manner and enable establishing daily and operator variations in staining. Read the full article

[twinkl22004]

“Genomic Coordinates”, Victor McKusick, Mendelian Inheritance in Man, 1966.

Here I present: “Genomic Coordinates”, Victor McKusick, Mendelian Inheritance in Man’, 1966. INTRODUCTION. The stain of DNA with giemsa intercalates between AT producing black color. The giemsa stain doesn’t intercalate between GC   and the white color remains.  Gray color is giemsa stain is neither AT or GC molecular DNA.  The table BELOW and molecular structures of DNA should help you…

[lobachemie1]

The Ultimate Guide to Stains for Microscopy: Techniques and Applications in 2024 

Microscopy is a cornerstone of modern biology and medical science, providing critical insights into the microscopic world. Central to the effectiveness of microscopy are stains, which enhance the contrast of biological specimens and reveal detailed structures that are otherwise invisible. In 2024, the landscape of stains for microscopy continues to evolve, with advancements in techniques and applications. This guide explores the latest developments in microscopy stains, including their techniques and applications. 

Understanding Microscopy Stains 

Stains for microscopy are chemical dyes or substances used to enhance the contrast of microscopic specimens. These stains bind selectively to specific cellular components, making them more visible under the microscope. The primary purpose of staining is to differentiate between different cell structures, tissues, or microorganisms, aiding in the detailed examination of specimens. 

Types of Stains for Microscopy 

Basic Stains: Basic stains are positively charged and bind to negatively charged cell components, such as nucleic acids and certain proteins. Common basic stains include Crystal Violet, Methylene Blue, and Safranin. These stains are often used for general cell morphology and bacterial identification. 

Acidic Stains: Acidic stains are negatively charged and bind to positively charged components, such as cytoplasmic proteins. Eosin and Acid Fuchsin are examples of acidic stains. They are useful for contrasting different types of cytoplasmic elements and tissues. 

Differential Stains: Differential stains are used to differentiate between various types of cells or cellular components based on their staining characteristics. The Gram stain is a classic example, distinguishing between Gram-positive and Gram-negative bacteria based on their cell wall composition. Other examples include the Ziehl-Neelsen stain for acid-fast bacteria and the Giemsa stain for blood smears. 

Fluorescent Stains: Fluorescent stains emit light of a specific wavelength when excited by a light source. These stains are used in fluorescence microscopy to highlight specific structures or molecules within cells. Examples include DAPI for DNA, FITC for proteins, and Phalloidin for actin filaments. 

Histological Stains: Histological stains are used for tissue analysis and are crucial in medical diagnostics. Hematoxylin and Eosin (H&E) staining is the most widely used histological stain, providing a clear distinction between cell nuclei and cytoplasm. 

Techniques for Using Stains in Microscopy 

Simple Staining: This technique involves using a single stain to highlight cell structures. Simple staining is ideal for observing cell morphology and general features. The process is straightforward: a few drops of stain are applied to a prepared slide, and after a short incubation period, the stain is rinsed off and the slide is examined under a microscope. 

Gram Staining: The Gram staining technique involves a series of steps including crystal violet staining, iodine treatment, decolorization with alcohol or acetone, and counterstaining with safranin. This method differentiates bacteria into Gram-positive and Gram-negative categories based on their cell wall properties. 

Fluorescence Microscopy: Fluorescence microscopy uses specific fluorescent stains to visualize cellular components. This technique requires the use of a fluorescence microscope equipped with the appropriate filters. Fluorescent stains bind to specific targets within the cells, and when exposed to light of a certain wavelength, they emit fluorescence, allowing for detailed observation of cellular processes. 

Immunohistochemistry (IHC): IHC is a technique used to detect specific proteins within tissues using antibodies tagged with fluorescent or chromogenic dyes. This method is particularly useful for identifying protein expression and localization in various diseases, including cancer. 

Confocal Microscopy: Confocal microscopy utilizes lasers and spatial pinholes to create high-resolution images of fluorescently stained specimens. This technique allows for optical sectioning of thick specimens and provides detailed 3D images of cellular structures. 

Applications of Stains for Microscopy in 2024 

Medical Diagnostics: In clinical diagnostics, stains for microscopy play a vital role in the identification and classification of pathogens, cancer cells, and other disease markers. Advanced stains and techniques enable more precise and early diagnosis of diseases, improving patient outcomes. 

Cell Biology: Researchers use stains to study cell morphology, organelle distribution, and cellular interactions. Fluorescent stains, in particular, allow for real-time monitoring of cellular processes and dynamics, contributing to a deeper understanding of cell biology. 

Histology and Pathology: Histological stains are crucial for tissue analysis in pathology. Stains such as H&E provide valuable information about tissue structure and abnormalities, aiding in the diagnosis of various conditions, including tumors and inflammatory diseases. 

Microbial Research: Stains for microscopy are essential in microbiology for identifying and classifying microorganisms. Differential stains help distinguish between different bacterial species, while fluorescent stains enable the study of microbial interactions and behavior. 

Pharmaceutical Research: In drug development, stains are used to assess the effects of new compounds on cellular and tissue structures. Techniques such as IHC and fluorescence microscopy help researchers evaluate drug efficacy and safety. 

Future Trends in Stains for Microscopy 

The field of microscopy stains continues to advance with new developments and trends. Some of the notable trends include: 

Development of Novel Stains: Researchers are developing new stains with improved specificity and sensitivity. These novel stains offer enhanced visualization of cellular components and processes, contributing to more accurate and detailed observations. 

Integration with Artificial Intelligence: The integration of AI with microscopy techniques is revolutionizing image analysis and interpretation. AI algorithms can analyze large volumes of stained microscopy images, providing insights that might be missed by manual examination. 

Enhanced Fluorescent Stains: Advances in fluorescent stain technology are leading to brighter and more stable dyes with reduced photobleaching. These improvements enhance the quality of fluorescence microscopy and enable more precise imaging of complex samples. 

Multi-Modal Imaging: Combining different microscopy techniques and stains allows for comprehensive analysis of specimens. Multi-modal imaging approaches, such as combining fluorescence and electron microscopy, provide a more complete view of cellular structures and functions. 

Conclusion 

Stains for microscopy are indispensable tools in the study of biological specimens, providing crucial contrast and detail that enhance our understanding of the microscopic world. As we move through 2024, advancements in staining techniques and applications continue to drive progress in various fields, from medical diagnostics to pharmaceutical research. By staying abreast of the latest developments and employing innovative staining techniques, researchers and clinicians can achieve more accurate and insightful observations, ultimately contributing to advancements in science and medicine. 

Whether you are a researcher, clinician, or student, understanding the latest techniques and applications of stains for microscopy is essential for making the most of this powerful tool. As microscopy continues to evolve, so too will the methods and stains that enhance our ability to explore and comprehend the intricate details of the microscopic world. 

[crimsonpublishersnutrition]

Organic Curcuma Caesia Roxb. Extract Induces p21 Expression and G0/G1 Cell Cycle Arrest in FaDu Oropharyngeal Cancer Cells

The oropharyngeal cancer is the popular medicinal uses of Curcuma caesia Roxb. (C. caesia) include the treatment of cancer. We aimed to evaluate the cytotoxic potential of C. caesia extract in oropharyngeal cancer cells. Total phenolic compounds and secondary metabolites of C. caesia extracts were determined by the Folin-Ciocalteu and UPLC-QToF-MS methods, respectively. Cellular analyses were carried out in an oropharynx cancer cell line (FaDu) and in a control keratinocyte cell line (Hacat). The cytotoxicity assay was carried out by reduction of 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT). Flow cytometry was conducted to evaluate cell cycle progression, and cell morphology was evaluated using Giemsa-May-Grunwald stain. Analysis of the intracellular Reactive Oxygen Species (ROS), Mitochondrial Membrane Potential (MMP), and the expression of p21, Bax and cytochrome c were conducted. The extract demonstrated dose-dependent cytotoxic activity in both cell lines. Increased retention was seen in the early stages of the cell cycle (G0 /G1) in FaDu cells. Moreover, FaDu cells showed strong alterations in their morphology that suggested cellular apoptosis and there was increased expression of p21 in that line. The treatment with C. caesia extract shows no significant differences in ROS, cytochrome c or Bax in both cell lines. C. caesia extract reduces cell viability and induces apoptosis in FaDu cells without deleterious effects in Hacat cells. To our best knowledge, there has been no other study that described such effect in cancer cells due to the treatment of organic C. caesia extract.

Read more about this article: https://crimsonpublishers.com/ntnf/fulltext/NTNF.000645.php

Read more articles: https://crimsonpublishers.com/ntnf/index.php

[moleculardepot]

Giemsa Stain for Cell Culture

Giemsa Stain for Cell Culture Catalog number: B2016012 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 1 g Molecular Weight or Concentration: N/A Supplied as: Powder Applications: a molecular tool for various biochemical applications Storage: RT Keywords: Giemsa Stain for cell culture, Endotoxin Grade: Biotechnology grade. All products are highly pure. All solutions are made…

[schlauemaus]

Babesiose

= Piroplasmose

Ursache

Babesien (Blutparasiten):

Übertragung: Zecke nimmt befallene Erys auf -> Vermehrung im Zeckendarm -> Befall von Ovar, Eiern und Speicheldrüse -> erneute Blutmahlzeit -> Sporozoitenvermehrung in Speicheldrüse -> Übertragung auf neuen Wirt (Hund) nach 12-36 Std

Babesien befallen Erys und zerstören sie direkt -> Freisetzung vasoaktiver Faktoren -> gesteigerte Kapillarpermeabilit��t, Gefäßdilatation, Hämostase und Hypoxie -> DIC mit postthrombotischer Azidose und Gewebenekrosen

große Babesien

B. canis: Überträger Dermacentor reticulatus; klinische Besserung meist innerhalb weniger Tage B. vogeli: Überträger Rhipicephalus sanguineus; Endemiegebiet Südeuropa; weniger virulent, kann für "schwache" Tiere trotzdem tödlich sein

kleine Babesien (selten, vorsichtigere Prognose)

B. gibsoni: bei uns meist Reiseerkrankung B. annae

Symptome

Inkubationszeit 10 Tage- 3 Wochen

(Per)akut:

perakut= Symptomauftritt in 24 Std

Mattigkeit, Inappetenz, Gewichtsverlust, Fieber

Erbrechen

Ikterus

stark vergrößerte Milz

blutiger, dunkler Urin: Hämoglobinurie (wg. massenhaftem Zerfall v. Erys)

Selten: gastrointestinale/ respiratorische Störungen, Ödeme, Aszites, Muskelentzündung, Oberflächenblutungen/ Epistaxis

bei schweren Fällen DIC -> tödlicher Verlauf

Zerebrale Form:

wg. Kapillarokklusionen -> Ischämien

Unkoordiniertheit, Paresen, Muskelzittern

Nystagmus

epileptiforme Anfälle

Bewusstseinsstörungen, Koma

Chronisch:

versch. lange Ruhephasen mit darauffolgenden Krankheitsphasen

rezidivierendes Fieber

Abmagerung, Teilnahmslosigkeit

Leberinsuffizienz, Ikterus

Laboruntersuchung

Blutuntersuchung:

ggr.-mgr. hämolytische regenerative Anämie mit Sphärozytose (wenn perakut noch nicht regenerativ) Thrombozytopenie Leukopenie (3/4tel der Fälle)/ Leukozytose (1/4tel der Fälle) mit Linksverschiebung und Monozytose Hämoglobinämie Hyperbilirubinämie Hypoproteinämie

Harnuntersuchung:

Hämoglobinurie Hyperbilirubinurie evt. Proteinurie mit hylinen Zylindern

Coombs-Test:

kann positiv ausfallen (vorübergehend)

Elektrophorese

wenn chron.: stark erhöhte Gammaglobuline

Diagnose

Anamnese: Auslandsaufenthalte?, Zeckenbefall?

Blutausstrich, Giemsa-Färbung: mikroskopischer Erregernachweis

PCR: am sensitivsten

Serologie: ELISA -> Feststellung von subakuten/ chronischen Fällen

Therapie

Transfusion

bei ausgeprägter Anämie (Hämatokrit <0,20l/l) mit Erythrozytenkonzentrat, Vollblut od. bovinem polymerisiertem Hämoglobin

Infusion

Antibabesien

va. kleine Babesien schwer zu therapieren Imidocarbdipropionat einmalig, wenn notwendig Wiederholung nach 14 Tagen Alternative: Phenamidin einmalig, wenn notwendig Wiederholung nach 48 Std

Prognose

je nach

Babesien-Stamm (B. canis gut, kleine Babesien schlecht)

Schweregrad Verlauf

Impfstatus

ungünstig:

Laktat, Triglyzeride und Phosphat hoch

Thrombozytopenie

mgr.-hgr. Anämie

Leukopenie

niedriges Totalprotein

Prophylaxe

Welpen aus Endemiegebieten: tw. Schutz durch maternale Antikörper

Ausbildung einer stammesspezifischen Immunität nach Krankheit -> hält nur ein Jahr, Hund bleibt Infektionsquelle

Zecken so früh wie möglich entfernen -> Übertragung erst in frühestens 12 Std

Zeckenprophylaxe: Spot-on (Akarizid)

Impfung: zweimal mit Abstand 2-3 Wo., Booster nach 6-12 Mo

Prophylaktische Gabe von Medis: Imidocarbdipropionat wenn zB Reise in Endemiegebiet bevorsteht, Schutz für 4 Wochen

[danref]

[oaresearchpaper]

The eye and its associated structures are uniquely predisposed to infection by the various microorganisms. The detection of infectious agents depends on the knowledge of the site of infection and the severity of the process because a variety of organisms cause infections of the eye. The present study was done to determine the bacterial, fungal and parasitic infections of the eye and also to assess the antibiotic susceptibility pattern of bacterial isolates in a tertiary care hospital over a period of 1 year. A total of 609 samples from clinically suspected ocular infections were collected and processed in the Microbiology department. Implicating pathogens were identified and isolated based on standard laboratory procedures. Antibiotic susceptibility was done for bacterial isolates using kirby bauer disk diffusion method. A total of 609 ocular samples were collected and processed, of which 247(40.5%) were positive either by culture and microscopy or microscopy alone. Bacteria were the commonest implicating pathogen accounting up to 215 (87%), followed by fungal and parasitic pathogens. Bacterial isolates were predominantly isolated from conjunctival swab and corneal scrapings. Maxim

[nethhiri]

Marooned: Chapter 45

Kid x FemReader x Killer

Warnings: mentions of kidnapping, rape, and forced pregnancy, sex workers, references to sex

Whispers

In the hallway of some nearby marine base, a man overheard some curious whispers, whispers about a woman with Y/C/H hair and a patchwork coat of 'justice', whispers about the Sea Snake being alive on some island. The man already knew you were alive, thanks to that sniveling Giemsa. He was a fucking idiot, but at least he had thought to tell the man about your resurgence. After that, there was radio silence, no sign of you anywhere. It made most marine captains nervous when you dropped off the radar in the past. You could pop up anywhere. It made the man, however, impatient, especially present time. He had been aching to get his hands on you. He knew Von Kossa was toying around with you too much. The man would have drowned you in acid if it were up to him. That would have ended you for sure. 

This time, though, he wasn't going to tell Von Kossa about this rumor. He would go check it out for himself. It was easy to see what islands would be your next stop. Maybe he would get some playtime in with you before handing you over to the boss, maybe even save him the trouble of killing you since he couldn't do it right the first time. He could even sell you. How ironic would that be? Or maybe he could keep you. No one had to know. He knew how much you loved torture and would be all too happy to oblige. 

All his partners were too weak to handle his proclivity for violence, but you had always been tough. And you had a pretty face, well, you used to. Even if it was ruined, your body was probably still how he remembered it. Yeah... he could keep you. How great would that be? It would be delightful to break you down. Manipulate you to beg for him, do anything he asked. Abruptly, a thought occurred to him. None of his partners lasted long enough to bear him a child and he remembered you being fairly formidable with haki. They would be perfect genes to mix with his. A slow grin spread over the man's face. Wouldn't that be wonderful? To fully subjugate you and get something out of it. You would be his diminutive, pathetic wife and you would love it. He would make you love it. And if you couldn't meet his needs, well then, he would turn you out to the crew and hand you over to Von Kossa after all. 

The man sat at his desk. He was broad-shouldered but not entirely broad himself, with musculature that stood out from under his clothes. His white-gray hair, half pulled up, and weathered, scarred skin betrayed his age. His chiseled, square jaw clenched. He wanted to chart a course right away, but he had gotten hot under the collar playing out this fantasy in his head. The man called Warthin palmed himself through his uniform pants, thinking of all the ways he was going to break you down. 

______________________________________________________________

Sleep had a hard time finding you. You tossed and turned with this strange anxious feeling. Your heart wouldn't slow down thinking about Kid asking you to stay. Of course you knew the answer, you did want to stay. It was something about the fact that Kid was asking you, though, that wouldn't allow your mind to rest. Maybe it was the fact that your relationship had been contentious for a long period of time. You pressed a hand to Mini's head, running your palm through her bristly fur. Sometimes petting her helped you fall asleep. Tonight was no such night. 

You threw on sweatpants to cover your bottom half, the top half covered by one of Killer's old shirts. Your feet shuffled you to the infirmary where you pulled out some books to flip through. They would either put you to sleep or you would learn something, a win-win. As soon as you sat down, there was a soft chime of metal on metal coming from your tag. Was that from you sitting or was that Kid? You tapped on the tag, spelling something in code: Y-E-S-?. Before transponder snails were popular, some people used this code to communicate. It jingled with more intention this time.

You opened the door to Kid's workshop a crack. At first it didn't seem like he was there, the lights were off. A small desk lamp turned on after a moment. Kid had his head in the crook of his elbow resting on the desk. His metal arm was laid on the table, like he had fallen asleep working on it. His red hair was limp over his eyes without his goggles to hold it up. It was endearing to see him all groggy without any of his normal gruffness about him. 

"Couldn't sleep either?" Your voice was quiet, matching the deadness of the night on the ship.

Kid yawned. "Almost was, but felt ya come next door."

"Sleep then. I'm not going anywhere." 

Kid blew his hair out of his eyes so you could see the yellow light reflected in them. 

You couldn't explain how this intimidating behemoth of a man was giving you puppy-dog eyes, but there was no other way to describe it. Sighing, you walked closer to him and let him pull you into his lap. His good arm curled around you and he rested his head on top of yours. After adjusting yourself to be comfortable, you cautiously reached to put your hand on his bad shoulder, assessing it. 

"If I could bring this back, would you want me to?"

"Nah." Kid's hand played with your hair. "S'part of my story.... and it makes me look badass." 

"I think it would be difficult for you to come off as anything less." You felt him laugh under you with a gentle bounce. Several minutes passed and you thought he fell asleep. 

"Sorry. Fer earlier."

"Yeah you better be. What if I had gotten amnesia?" You slapped his chest.

"S'easy." Kid was getting heavier as he let himself drift to sleep. He was struggling to keep his eyes open. "Would just have tah use m'devilish charm... to ..." There were the beginnings of a rumbling snore deep in his chest.

"Kid?" You tapped him.

"...until ya loved me again...." His eyebrows furrowed in that half-dream state and he yawned. "Liked? Loved?" It was like he was talking to himself, or saying his thoughts out loud. "I dunno.... whatever ya want. M'sleepin." His arm tightened around you. 

You blinked slowly. Is that what he thought? That you...? Do I?  You were in a stunned state. No way. No. Maybe you could go as far as saying you liked him. Fine. You could admit that much. He was fucking hot and amazing in bed, easy to like. But anything more than that? Now your heart was racing faster than ever and you were trapped against Kid until he woke up or moved his arm. 

You felt similarly about Killer, and Killer had the additional features of actually being sweet and thoughtful. He even tried to defend you somewhat, in the beginning, against Kid's impulsive wrath. Theoretically, if you did want to pursue something, which one would you pursue? They were fine with sharing casually. Would that change if it was a less casual arrangement? Really what would change other than the label applied to it? 

At some point in the night, you must have managed to fall asleep, because you woke up on top of Kid, who seemed to have fallen backwards off the bench. His feet were still propped up on it. It was dumbfounding how he could sleep through almost anything. You must have been tired since you technically also slept through it. Your back was definitely going to hurt from the strange sleeping position. 

"G'morning." Kid stretched. He must have woken up before you, shockingly. He pressed his lips to the top of your head.

A kiss? A kiss unrelated to horniness? "Hey."

"Sleep ok?"

"No. It was very uncomfortable in your vice grip."

"If I'm so uncomfortable why are ya still on top of me?"

You pushed yourself to get up and was swiftly pushed back down and trapped again in Kid's arm.

"No, no. M'not ready fer ya to get up." 

You huffed and relented, shifting to attempt getting comfortable.

Kid's arm relaxed around you and his hand traveled up the back of your shirt to rest on the skin. "Will ya stay?" He asked softly, in a tone lacking his usual confidence.

You moved your head to look at his face. "Are we still talking about cuddling?"

Kid scoffed. "This ain't cuddling. We're just hanging out." Still, he squeezed you slightly tighter to himself. You felt him take a deep breath under you. "I'm askin if- Do you think- Would you- Do you want to be a Kid Pirate?" His eyes avoided yours until he got the question out.

"Do I want to be your pirate?"

His face went as red as his hair. "Not my pirate. A Kid Pirate...Unless-"

"Are the Kid Pirates not yours?" You were fucking with him, though you wished you hadn't cut him off so quickly. What was he gonna say?

Kid squeezed the breath out of you. "Yer such a fuckin smart-ass. Always hafta be a rotten brat."

You wheezed, "Yes, but I'm your rotten brat." You realized how that sounded and quickly amended it. "'Your' as in, you know, the collective Kid Pirates... Not just..." You coughed, your voice quiet from lack of air. "You." You tapped Kid's arm and he released his hold so you could get a breath in. You re-inflated your lungs to finish speaking. "As in, yes, I would like to be a Kid Pirate." You pushed yourself up, putting one leg on either side of Kid, sitting on his stomach.

A very genuine, relieved grin appeared on Kid's face. "Welcome officially, Doc Rotten." 

"I am the very loosest definition of doctor."

"Yer tight to me." Kid's grin turned more sinister. 

"Oh come on. Can it wait until after breakfast?" You pouted. "I'm hungry."

Kid pulled your hips closer to his face. "I got breakfast right here." The man couldn't ask you a simple question but he would eat your pussy at the drop of a hat. 

Needless to say, you were both late for breakfast. You didn't even bother to smooth out the sex hair or change into real clothes because you were starving at this point. 

"Are those my pants?" Killer eyed the overly sized gray sweats you had on. "I've been looking for those."

"Ah yeah I borrowed em. I'll give em back." You sat down with food. "They're gonna need to be washed though." 

You could tell Killer was frowning behind his mask. 

Kid slapped him hard on the back, causing Killer to grunt. "Lighten up, Killer. It's not the first time my cum was all over the inside of yer sweats."

You also frowned. The entire table didn't need to know what exactly was going on under your borrowed pants, that were definitely going to be stuck to your leg afterwards. You apologetically smiled at the first mate.

Kid banged his fist on the table. "Forget that. We got ourselves a permanent new addition!" He pointed at you with a crispy piece of bacon.

"So you're one of us officially?" Heat asked.

Killer perked up next to him. "Oh you finally grew a pair and asked?" 

"Shut up, Killer. They're plenty big."

"Probably considerably smaller after this morning," you quipped with a smirk. 

Wire was smirking similarly under his hood. 

Kid announced to the crew that they would be celebrating your official induction tonight. The next island was famous for brothels, good booze, and whatever other trouble you could get into. The crew hustled after breakfast to finish their assigned duties for the day so that they could have time to prepare for tonight or get going as soon as the ship was docked. 

______________________________________________________________

It wasn't usually your vibe to wear sexy or revealing clothing, opting more for practicality than anything else. But tonight was special. You had on full-length, maroon pants, though the pants were crafted such that their entirety looked like if jeans had all their vertical threads removed. Only thousands of thin, cotton fibers going horizontally covered your skin, leaving it exposed between the threads. They came together on the sides into fringe. Essentially, they were pants consisting entire of fringe, with excess lying the sides. The only solid fabric was in a thong shape, to cover your front. The top was a matching maroon harness-style top with straps that accentuated your bust and the shoes matched, too. You completed the monochromatic look with maroon lipstick and smoky eye, rather, Emma and Quincy helped you. 

Mini met you on deck with an enthusiastic nudge of her wet nose, ready to get fed especially good table scraps. You thought Mini was getting the majority from the girls or Killer, but the boar quickly zeroed in on the real weakest link. Wire, it turned out, had an incredibly soft spot for animals. Once, you even saw him feeding the ship mice from his palm. When Mini wasn't hovering around you, she was usually with him. 

"You better get off the ship before Captain sees you in that or you'll never make it off the ship." Heat came up behind you as you stood on deck. 

"Is it too much?"

"Yeah but you look hot so who cares?" Heat grinned. "You look like a Kid Pirate." 

You grinned at him. You did feel good in this outfit, even if it was slightly out of character for you to wear so little. It made you feel powerful in a different way. You knew these pants made your ass look perfectly rounded and showed off the smooth, soft skin of the cheeks. And you did occasionally wear tight shirts, but this top did a lot more for presenting your breasts in the way of cleavage.

"Heat! Ya dog! Ya already brought back a whore?" Kid was behind you both and couldn't see your faces. 

"Actually, I was just leaving," you partially turned so he could hear you before jumping down, Mini hot on your heels. It was hilarious to you that Kid mistook you for a whore. 

"Why was the pig followin her?" Kid came to stand next to Heat.

"Boss, that was Y/N."

It took a second for Kid to put two and two together. "That was Y/N?!" Kid didn't say anything more before going after you. 

You went off on your own, looking for somewhere fun. It was fairly crowded, so you were carried away in a sea of people almost immediately. You ended up checking out a few bars, seeing several of the crew scattered around. You sat with some of the girls for a drink or two at one of the brothels that had a fine selection of men to choose from. None of them were as beefy, crass, or handsome as your particular interests at the moment. Though, that didn't stop you from helping get your friends laid. When you were the last one at the table, you left with Minerva.

There were a few catcalls that faded into the background, not worth your time to respond. Some got ballsy and came directly to you. You shut them down hard and if they didn't take the hint, you beat them down harder. You heard running feet behind you and prepared to throw punches, but stopped as you recognized Killer and Heat. 

"Easy, tiger." Killer put his hand on your head and roughed up your hair. "We gotta do something bout that observation haki of yours."

You huffed. "I can handle myself fine without it."

Heat smoothed your hair back out from behind before walking by your side.

"Thank you, Heat. Such a gentleman." You could see that Killer's mask was ever so slightly tilted in your direction and he was lagging behind you a little. "You might as well walk behind me if you want to look." 

"While I would love to slow down and appreciate the view, Kid heard about an exclusive pirate-only club and wants us to go. Supposed to be the best on the island. Best in the area. The Snake Den."

"Because Snake Hole was too on the nose," you laughed at your own joke.

Once you were in the right part of town, a few more minutes of walking, the place was easy to identify, especially if you knew the name. A giant stone snake head was the entrance. Its mouth was open in a hiss and two large fangs served as pillars on either side of the walkway. A red glow emanated from inside. They were famous for only allowing one crew in at any given time, and the wide variety of kink they would cater to. All who were employed were the best of the best, by skill and looks. 

Wire and Kid were waiting outside, as were a few others, including Pomp, Reck, and Bubblegum, who looked nervous. None of the ladies were with them, probably still with their own rented lovers for the night. Kid looked impatient as always, and annoyed with you, as always. 

"Always fuckin disappearing," He chided you. "Took ya long enough." Kid addressed the entire group. "Been waitin for ages! Let's go!" 

Kid entered and you followed behind him and the other officers. Every brothel they visited, there was a chorus of women excitedly whispering about the Kid Pirates, which evolved into them fawning over the crew, and hanging all over them. It was partially the business, however, you had noticed the crew had a reputation for treating those who worked in brothels very well. The behavior was more the behavior of a fan than someone trying to get money out of a customer. If you listened closely to the whispers, they were all about how well they tipped, how well they were equipped, and how fun they were to be around. This was no different. They had never been here before, though women were addressing them by name and seemed to know a lot about them. Some of the women may have been from other brothels, the others may have just learned through simple gossip. Brothels often had partnerships with each other where women could be swapped, either to keep the selection fresh or to let women be closer to their favorite lovers. This kind of trading was all consensual and usually suggested by the working girls themselves.

Incredibly beautiful women were already hanging off the arms of the officers, with quite a few around Wire. These women had obviously heard rumors about his size. They were standing in a lobby area. The lights were dimmer in the back, where several hallways led. At the front of the lobby, a tall, slender woman ushered everyone into the main dining area, where hot food was already on the table and women were lined up waiting to pour a glass of the guests' beverage of choice. All the working girls were dressed in various silks, many of them were robe-like with promises of something skimpy underneath. The room was cozy and had many comfortable sitting options. You had assumed the woman in the lobby was the madame. A different woman, clearly in charge, entered, personally greeting every pirate by name, asking a few questions, and calling over a girl she thought would suit their needs. This woman was also tall, with a touch more curve, pale with auburn hair that flowed down her back and muted green eyes. She was hiding it well, though she had a slight limp that made her gait unique.

Your eyes lingered on the way her steps were long and exaggerated the way her hips swayed as she came to you. You had seen it before somewhere, though you didn't frequent brothels. A wave of jasmine flooded your nose as she guided you away from the room, towards the hall just outside. Her hands cupped your face, and turned it, studying your features.

"Please forgive me." The sultry look was fading from her face and was replaced with one reflecting deep sorrow. It took you aback. "You look like someone I knew once." A thin smile attempted to cover whatever memory was surfacing for her. "I don't recognize you as a Kid Pirate. Are you new? What sort of things are you interested in? I'm sure I have just the girl." 

You took her shoulder, gently turning her. She stiffened and started to protest when you moved her hair from her back, careful to only peek. There was a brand between her shoulder blades that you knew would be there. "Jade?" You guessed softly. She was named after the color of her eyes. You covered the scarred half of your face with your hand, helping her out.  

"It's Madame Tempest now..." she said quietly. Her eyebrows knit together and you saw her face brighten with recognition. "Y/N?!" She covered her mouth, shrieking it far louder than she meant to and grabbed you for a tight hug.

In the other room, about half of the other women turned with surprise in their faces towards the outburst, excusing themselves for a moment.

Next Chapter

Tag list: @bbnbhm @nocturnalrorobin

[midseo]

Methyl Diethyl Safranine, Methyl Diethyl Safranine Manufacturer, India

Methyl Diethyl Safranine : We are Methyl Diethyl Safranine Manufacturer, Methyl Diethyl Safranine Exporter, Supplier in Mumbai, India. Call Now.

Methyl Diethyl Safranine, Chemical, Chemicals, Electroplating Chemical, Electroplating Chemicals, Lab Chemical, Lab Chemicals, Laboratory Chemical, Laboratory Chemicals, Industrial Chemical, Industrial Chemicals, Safranine, Safranine O, 7 Diamino 2, 8 Dimethyl 5 Phenyl, Phenazinium Chloride, Diethyl Safranine, N Diethyl Phenosafranine, 3 Amino 7 dimethylamino, Basic Violet 5, Basic Violet 8, Thioflavin T, Basic Yellow, Basic Yellow I, O Cresolphthalein, Thymolphthalein, Biological Stain, Biological Stains, PH Indicator, PH Indicators, Lab Reagent, Lab Reagents, Laboratory Reagent, Laboratory Reagents, Dyes & Chemicals, Dye & Chemical, Dyes & Chemicals, Acid Fuchsin, Alizarin Yellow, Alizarin Yellow GG, Alizarin Yellow R, Aurin, Rosolic Acid, p-Rosolic Acid, Azure A, Azure B, Azure 11, Azure 11 Eosinate, Barium Diphenylamine Sulphonate, Basic Fuchsin, Biebrich Scarlet, Brilliant Green, Bromocresol Purple, Bromocresol Purple Sodium Salt, Bromophenol Blue, Bromophenol Blue Sodium Salt, Bromophenol Red, Bromothymol Blue, Bromothymol Blue Sodium Salt, Calcon, Solochrome Dark Blue, Calconcarboxylic Acid, Calmagite, Carbol Fuchsin, Chromotrope 2B, Chromotrope 2R, O Cresolphthalein Complexone, Cresol Red, Cresol Red Sodium Salt, 2:6 Dichlorophenol Indophenol Sodium, 4',5' Dibromofluorescein, Dimethylglyoxime GR, Eosin Blue, Eosin Yellow, Free Acid Eosin Yellow, Water Soluble Eosin Yellow, Eriochrome, Solochrome Black T, Ethyl Orange, Evans Blue, Fluorescein, Fluorescein Complexone, Calcein, Fluorescein Sodium, Giemsa Stain, Hydroxy Naphthol Blue, Indoine Blue, Leishman Stain, May & Grunwald Stain, Methyl Blue, Aniline Blue W/S, Methylene Violet 3Rax, Methyl Orange, Methyl Red, Methylthymol Blue Complexon, Methyl Violet, Naphthol Green B, Naphtholphthalein, 1 Naphtholphthalein, Neutral Red, Orange G, Nitroso R Salt Gr, Indole 3 Acetic Acid, New Fuchsin, asia, asian, india, indian, mumbai, maharashtra, industrial, industries, thane, navi mumbai, pune, nashik, aurangabad, ratnagiri, nagpur, ahmednagar, akola, amravati, chandrapur, dhule, jalgaon, raigad, sangli, satara, belgaum, kolhapur, belgaon.

[twinkl22004]

“Giemsa Stain Chromosomes”, Victor McKusick, Mendelian Inheritance in Man, 1966.

Here I present: “Giemsa Stain Chromosomes”, Victor McKusick, Mendelian Inheritance in Man’, 1966. INTRODUCTION. I am going to explain “Geimsa Stain”. AT/TA shown atop stains BLACK because intercalation.  GC/CG appears WHITE unstained.  TA/GC is partial intercalated GRAY.  Using light and electron microscopy Geimsa stain produces “banding”. Think of it as your left hand. The space between the…

[vehicles-are-vesicles-for-people]

Giemsa G banding experiment