#Cell Line Development

[catboybiologist]

oh dear. it seems that i am a -quickly looks at bio- teeny tiny protozoan unknown to science i hope a beautiful woman studies me and learns my teeny tiny protozoan secrets.

Damn I hope there's a gorgeous protozoa researcher out there to fulfill those wishes bc every model organism I've worked with has been in animalia

[sylviaplathenthusiast]

my brain only uses its cognitive capacities for evil

[meditechinsight01]

Global Cell Line Development Market: 8-10% CAGR Growth and the Future of Biopharmaceuticals by 2029

The cell line development market is growing at a CAGR of approximately 8-10% due to the increasing adoption of cell-based therapies. Key drivers include the rising demand for monoclonal antibodies, advances in bioprocessing technologies, growing focus on personalized medicine, and increased R&D investments in biopharmaceuticals. However, challenges such as high costs, lengthy development timelines, and regulatory complexities restrain market growth.

Cell line development is the process of generating, selecting, and optimizing a population of cells capable of consistent growth and production for various biological and pharmaceutical applications. These cell lines are "factories" producing biologics such as monoclonal antibodies, vaccines, enzymes, and other therapeutic proteins. Key steps in cell line development include:

Cell Isolation: Selecting a specific cell type from a primary source (e.g., human or animal tissue)

Genetic Modification: Introducing desired genes into the cells to produce the required proteins or enhance their performance

Screening and Selection: Identifying and isolating cells with high productivity, stability, and desired characteristics

Optimization: Adapting cell growth conditions, such as media composition and culture techniques, to maximize yield and quality

Validation: Ensuring the cell line meets regulatory and production standards, including consistency and scalability

Cell line development is essential in the biotechnology and pharmaceutical industries, where robust, stable, and high-yielding cell lines are critical for the efficient manufacturing of biological products.

Discover the more details-Download the PDF brochure :

The Role of Biologics in Driving the Demand for Cell Line Development

The surging demand for biologics, including monoclonal antibodies, vaccines, and cell-based therapies, is a significant force driving the market. Biologics have transformed the treatment landscape for chronic diseases, including cancer, autoimmune disorders, and genetic conditions, due to their targeted and highly effective action. This demand has necessitated robust cell line development platforms capable of producing high-yield, stable, and compliant cell lines to meet large-scale production needs. Additionally, the rise in biosimilars and the expansion of biologics in emerging markets further amplify the need for efficient cell line development solutions. Continuous advancements in upstream bioprocessing technologies complement this demand by enhancing yield, stability, and scalability, ensuring biologics manufacturing remains sustainable and profitable.

Integrating Synthetic Biology and AI for Next-Generation Cell Line Development

Synthetic biology advancements have revolutionized the engineering of cell lines, enabling precise genetic modifications to optimize productivity and functionality. Technologies such as CRISPR-Cas9 and TALEN allow scientists to introduce targeted changes, enhancing the metabolic and growth profiles of cell lines. This has led to the generation of cell lines with improved yields, reduced batch variability, and faster development timelines. Furthermore, integrating automation and AI into cell line development has streamlined the selection process, increasing efficiency and reliability. These advances not only reduce costs but also ensure compliance with stringent regulatory requirements, paving the way for more accessible and scalable biopharmaceutical production. The incorporation of synthetic biology tools continues to unlock new potential in the market, meeting the growing demand for innovative therapeutic solutions.

Competitive Landscape Analysis

The global cell line development market features both established and emerging players, including Thermo Fisher Scientific Inc.; Danaher Corporation; Sartorius AG; Merck KGaA; Lonza; Creative BioLabs; WuXi PharmaTech; Advanced Instruments; and Bruker Cellular Analysis (Berkeley Lights) among others. Some of the key strategies adopted by market players include product innovation and development, strategic partnerships and collaborations, and geographic expansion.

Unlock key findings! Fill out a quick inquiry to access a sample report

Global Cell Line Development Market Segmentation

This report by Medi-Tech Insights provides size of the cell line development market at the regional- and country-level from 2022 to 2029. The report further segments the market on the basis of product & service, source, cell line, and application.

Market Size & Forecast (2022-2029), By Product & Service, USD Million

Reagents and Media

Equipment

Automated Systems

Centrifuges

Bioreactors

Storage Equipment

Others

Accessories and Consumables

Services

Market Size & Forecast (2022-2029), By Source, USD Million

Mammalian Cell Line

Non-Mammalian Cell Line

Insects

Amphibians

Market Size & Forecast (2022-2029), By Cell Line, USD Million

Recombinant Cell Lines

Hybridomas

Continuous Cell Lines

Primary Cell Lines

Market Size & Forecast (2022-2029), By Application, USD Million

Bioproduction

Drug Discovery

Toxicity Testing

Tissue Engineering

Others

Market Size & Forecast (2022-2029), By Region, USD Million

North America

US

Canada

Europe

UK

Germany

France

Italy

Spain

Rest of Europe

Asia Pacific

China

India

Japan

Rest of Asia Pacific

Latin America

Middle East & Africa

About Medi-Tech Insights

Medi-Tech Insights is a healthcare-focused business research & insights firm. Our clients include Fortune 500 companies, blue-chip investors & hyper-growth start-ups. We have completed 100+ projects in Digital Health, Healthcare IT, Medical Technology, Medical Devices & Pharma Services in the areas of market assessments, due diligence, competitive intelligence, market sizing and forecasting, pricing analysis & go-to-market strategy. Our methodology includes rigorous secondary research combined with deep-dive interviews with industry-leading CXO, VPs, and key demand/supply side decision-makers.

Contact:

Ruta Halde  Associate, Medi-Tech Insights  +32 498 86 80 79  info@meditechinsights.com 

[mitalipingale]

The Stable Cell Line Development Market in 2023 is US$ 5.29 billion, and is expected to reach US$ 11.36 billion by 2031 at a CAGR of 10%.

[rahulp3]

Scope of the Cell Line Development Market Report By 2033

With a 7.7% compound annual growth rate (CAGR) from 2023 to 2033, the global cell line development industry is projected to reach US$ 5.06 billion in 2023 and reach US$ 10.6 billion by 2033. The market was estimated to be worth US$ 4.7 billion in 2022.

The substantial growth of the cell line development market is predominantly attributed to the increasing adoption of bio-therapeutics for treating chronic diseases like arthritis, diabetes, and cancer. This surge in demand is driving the need for automated cell line development and cell line development services.

Get a Sample Copy of the Report:https://www.futuremarketinsights.com/reports/sample/rep-gb-7566

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[highdramas]

ik you said you were looking for ideas: what if the f!attending!reader got too drunk at a group outing and he took her home?

party for you | dr. jack abbot

pairing: jack abbot x f!resident!reader warnings: language, age gap (unspecified, but reader is late 20s/early 30s and jack is mid/late 40s), descriptions of throwing up, references to sex and gets steamy but nothing explicit, sweet sweet fluff <3 word count: 2.6k summary: you party a little too hard and jack takes care of you in his perfect way. notes: if you are under 18 do not interact with any of my work or this fic. thank you for this lovely request!!!! idk if i like how this one turned out but i hope that you do! oh to be taken care of by jack when i'm too drunk! this is a part of the ring of fire interconnected series, but it’s not necessary to read the prior parts to understand this fic. if you would like to, though, you can find the masterlist here <3 not proofread so apologies for any errors!

“hey, brother.”

“hey man.” jack keeps one steady hand on the wheel, the other holds the phone to his ear. when he felt the vibration from his pocket, there was that sudden, jolting feeling. was it you? were you okay? did you just want to say hi? did you miss him, the way he missed you? but then robby flashes across his screen, adorned with a stupid picture of him from a thanksgiving potluck a few years back.

( when he thinks about that thanksgiving potluck now, in retrospect, the detail that stands out to him is that he actually sat next to you. when dana, half drunk and wild with her wine glass in the air, asked everyone to share what they were grateful for, jack had nudged you and muttered, “just say your family. it makes her weepy, every time.”

you had covered your mouth to stifle your laughter. he felt entirely satisfied with himself. one little moment out of several across the years where the lines were clearly drawn: attending, resident. teacher, student. off limits. )

jack makes a shoddy attempt at shaking the thought of you away to focus on whatever it is robby needs to share– unlikely to keep him as rapt, but worthy of his time and attention, presumably.

“you getting close to town?”

“uh– ‘bout five minutes out. what’s up?” the rumble of the party is heard, but robby doesn’t say anything. jack asks robby if you’re alright. more quiet.

when you asked jack last weekend if he was planning on going to princess’s halloween party, with that goddamn glint of hope in your eye, it had taken all of the self control in the world to say, “i volunteer with the VA for a camping trip every halloween weekend. i won’t be back until late the night of the party.”

you had deflated slightly, but mustered up enough mischief to say, “what, you don’t want to go as jim and pam from the office, in true 'we just started dating' fashion? rude.”

he had hung his head, put his hands on your thighs and looked up at you with what he could only assume was a pathetic, pitiful expression. to be fair, you made him reasonably pathetic, and certainly pitiful. the fact that every man wasn’t unreasonably infatuated with you was a wonder to him.

“i’m sure i can get someone to cover for me–”

“no,” you had said with a shake of your head. your hands went to cradle his face, your thumb brushing the place where a dimple develops when you really get him smiling, or laughing. “don’t do that. they’re planning on having you. i shouldn’t be selfish.”

but what if he wanted to be selfish? it’s already been a significant struggle to not want to follow you everywhere. he would go with you into his worst nightmare if you asked. he’d do another tour if it meant that you were smiling at him, just like you were in that moment. the hold that you have on him is not lost on him in the slightest, and he wouldn't change it, even if he wanted to. devotion feels good. it feels right.

but, with coaxing from you, he had gone on the trip, and you still went to princess’s party. when he got cell service back during the four hour drive, he immediately called you, if only to hear your voice. you sounded excited, but he could hear the dip in it when you said wish you were here. then you sent a picture of that goddamn tomb raider costume…

which was why, despite his exhaustion, despite the fact that he probably stinks like a campfire, despite the way that his leg burns… he has princess’s address in his gps.

“is she alright?” jack repeats himself.

“she’s fine. she’s fine. she’s just had a lot to drink, is all. dana’s in the bathroom with her now, but i think she’s about ready to, uh, go. i was just wanting to see if you were close, or if we should get mateo to–”

“don’t put her in a goddamn car with mateo, or anyone else. i’m down the fucking road. are you keeping her hydrated?”

“yes, and–”

“what about some carbs? get her a piece of toast. she’ll ask for it with honey, but best to keep it plain. and maybe some ice on her wrists to cool her body temperature. she’s prone to overheating.”

“jack,” robby cuts through. “we know. we got her. i’ll see you soon, brother.”

–

the thing that people don’t tell you about alcohol? it’s fun when it’s fun. it’s horrible when it’s horrible. well, they do tell you that. you've seen enough alcohol poisoning cases come through the emergency department to know just how easy it is to topple over the edge without even realizing it. but, treating it is one thing. experiencing it is another.

and there might not be anything more hellish than getting nearly blacked out, surrounded by health care professionals.

everything had been great. you were drinking a little more than normal, but, hey– it was halloween! and you had two days off in a row! that felt like something to be celebrated. and, yeah, maybe the fact that you drank two of those nearly neon blue buzzballs was a bad idea. maybe that last shot with princess was also a bad idea. but you had been feeling good. better than good.

you had never been much of a drinker, or a partier, but not because you didn’t want to be. sometimes, you just didn’t know how to be. at a certain point, you had accepted that you were a little bit of a recluse in college… and med school… and, now, through your residency, too. you liked working and researching and if you weren’t doing either of those things, you preferred to smoke some weed to relax and watch below deck, or a real housewives franchise.

the only time that you didn’t feel like a loser was when you were in jack’s living room watching something inane, like one of those live police shows, or ancient aliens, because if you get jack high enough, he’ll want to watch it for hours. in those moments, the only label that felt sufficient was his.

you are typically wise enough to know what your limits are around drinking– you are a doctor, after all. but you were feeling a little moody since jack wasn’t there, and the alcohol wasn’t doing anything to help that, and then, you made the uniquely horrible decision to hit the joint that was being passed around the backyard. you told yourself it would calm your nerves. the only thing it did was kick off the world spinning for the better part of an hour.

which is how you’re now, here. back against the wall of princess’s bathroom, your head between your knees, with dana and samira crouched beside you, each trying to heal you– as is their nature.

“baby, you need to look up, and just stare at that shampoo bottle. keep staring at it and don’t stop. that’s my trick for the spins.” dana’s voice sounds warbled, far away. you force your head up and stare at the shampoo bottle in question. “good, good. keep doing that.”

robby comes to the doorway with his phone in his hand, and a fresh glass of water in the other. you don’t quite pick up everything that he says, but you hear jack and soon and it’s enough for your incoherent brain to string together the rest. “jack is here?” you ask, and damn that hope in your voice, damn it to hell. you’ve been publicly dating for less than six months and you’re already being embarrassingly, publicly in love.

“yeah, he’s almost here.”

you smile. laugh a little to yourself. dana and samira share a look and they can’t help the laughter, too. you’re about to say something else when robby is maneuvered to the side, and jack stands in the doorframe. everything is still spinning, but you try dana’s trick, making jack into your shampoo bottle, using the image of him to ground yourself. “hey,” you say, head falling back and thunking against the wall. he’s at your side immediately, using a hand to cradle the back of your head, push strands away from your eyes. “i think i partied a little too hard.”

“yeah, i think so too, kid.” he gives robby an intense look and a nod of his head and everyone clears out from the bathroom, shutting the door behind them. he finds your eyes, so bleary and tired and unfocused. you give him a half hearted pout that turns into a smile. “want me to get you home?”

“yes, please,” you let him hold your head up, hand sliding from the back of it to your cheek. you turn your mouth to press a kiss into his palm, open-mouthed, wanting, despite everything. “i’m so embarrassed.”

“what?” jack scrunches his face up. “you have nothing to be embarrassed over.”

“i do,” you say through a hiccup, your hands reaching to grab at jack’s forearms– you love his arms, love how strong they look, love how they hold you. “i started blabbing about how much i love you to robby. and i’m not embarrassed that i love you– i’m embarrassed because i’m supposed to be a professional, and you’re my attending, and–” you cut yourself off because he’s smiling at you, wide. “why are you looking at me like that?”

“because you’re one of a kind, kid.” he presses a kiss into your forehead. “c’mon, let’s–”

the sentence dies because you dive forward, scrambling to get the toilet seat up before you empty the contents of your stomach into the toilet. “shit.” you hear him grunt, but it’s faint over the sound of you coughing, resting your forehead against the edge. you take in a shaky breath as you feel jack’s hands pulls the strands of your hair that have escaped the lara croft-esque braid you put your hair into. “let it out. you’re good, i’ve got you. you’ll feel better.”

you throw up another two times before your stomach finally relents and gives up the good fight. your limbs are shaky, and jack’s hand rubs a gentle pattern into your spine. you faintly, far away, hear the door open just a crack. jack murmurs something to who you can only presume is robby, because he returns with a fresh glass of water, a sleeve of saltine crackers, and a stick of gum. you're just glad they're not taking you to the emergency room for a bag of fluids. you wouldn't put it past them.

jack settles behind you with a groan, knees creaking. you look at him from over your shoulder and ask, despite everything, “how was the camping trip?”

he smirks, the amusement at your question evident on his face. “good. really good.” he adjusts the tank top that you wear, where it’s ridden up, smoothing it against your waist. “how are you feeling?”

“much better,” you say with a nod of your head as a punctuation. “it was the joint i smoked that really did me dirty. and the fact that i ate, like, ten hush puppies.”

“i believe it.” he looks lost in thought for a moment, before he adds, “i’m sorry i wasn’t here.”

“it’s not your job to babysit me,” you muse, shaky hand taking the water from him. he shakes his head and puts it to your lips, helps tip your head back, takes proper care of you. “and you deserve to do things on your own. we both have to do that if we want this to work.” the alcohol still has you loose, because you continue, “i like being my own person. doing my own things. but…” you shrug a shoulder. “i really like being me with you. i did wish you were here tonight. i missed you. i kept looking over my shoulder like you'd just... be there.”

“i wished i was too. i missed you,” jack sets the glass down and hands you a saltine cracker instead. his eyes trail you, up and down. “i didn’t get a chance to say it yet, but you look sexy.”

“i just threw up enough alcohol to get an entire sorority drunk.”

“still sexy.” his hand lands on your thigh and squeezes one time before he lets go. “you wanna get out of here? or want to hang for a little?”

“i’m ready to go.” jack helps you to your feet and rubs at your shoulders while you take handfuls of water from the sink, swishing it about in your mouth. jack unwraps the stick of gum and turns you, tapping it against your chin once. you open your mouth for him and he places it on your tongue with a heady gaze. you blow a bubble at him, listen to the pop fill the air around you.

when his eyes flick down to your mouth, you put a hand on his chest. “you can’t wait to kiss me until i’ve brushed my teeth?”

“i don’t care.” his hands are getting greedy, going to your waist, squeezing like he just needs to feel you, needs to feel that you’re real and right in front of him. “please.”

before, you never would’ve pinned jack abbot as a clingy man. now, it makes perfect sense to you. you only need to be apart for a few hours before he’s looking at the little picture of you that he has in his wallet. you could only imagine him these last three days: huddled around a campfire, mind drifting to you. the thought makes you feel a satisfaction you've never known before.

you don’t know if you’ve ever been loved as completely as jack loves you. it’s not just one way that he’s good: it’s all of the ways. he’s not perfect, and you don’t expect him to be– sometimes, he can close himself off. he can snap at you when he’s irritable. but he’s good. a good man.

how can you deny jack what he’s asking for? especially when he asks so nicely. you give a slight nod of your head and that’s enough for him. he pulls you in close, by the back of your neck, his favorite point of your body: and he presses his lips against yours and kisses you so thoroughly that you feel breathless.

you feel a little bit like a college kid, making out in a bathroom while loud music plays behind you. but you wouldn’t change a thing: you love sex with jack, you love that he makes it good, tender, hot, passionate. but you love this, too: the neediness that can exist without ever taking an article of clothing off, arching your back so that your breasts press against his chest, hearing the catch of his breath when you do. you love to rake your fingers through his hair because you know that it drives him crazy, and you like seeing the goosebumps that you get in return.

jack nips your lower lip with his teeth and it makes you gasp into his mouth, and his hand squeezes against the back of your neck. he chuckles into you. “i love when you do that,” he breathes. “when you make that sound.”

it’s your turn to cling to the back of jack’s neck and press your fingers into it. “are you going to take me home or not?”

jack wears his smirk like a badge of honor. and then, so seamlessly, he blows a bubble with the gum that at some point went from your mouth to his.

“that is disgusting,” you say, but your face deceives you: your slack-jawed, twinkly eyes expression says everything that words can’t. “foul.”

“sorry,” jack says and comes in for one more hot kiss, using his tongue to press the gum back into your mouth. “didn’t mean to take that.”

[kekewrites]

How to make a baby 101

Tw. dubcon, dark content, virginity loss, breeding kink, creampie, size difference, a lil age gap (2-4 years), sex education gone wrong, cunnilingus, coercion, nicknames, creampie, overstimulation, corruption, reader is ignorant and innocent (sheltered, kinda went a bit mute at the snusnu part), nerdy to cocky (character)

***

Step 1: Ask your childhood friend to help you study for your exam.

"Hmm? How to make a baby?" His eyes widen a little, a small flicker of something in his eyes as you ask him about reproduction system or along that line.

"Well," he said slowly, his voice still composed but with a hint of surprise, "that's not quite how it works." He paused, choosing his next words carefully. "Making a baby is a bit more...complicated than that."

Sitting cross-legged on the bed, you listened attentively as he explained the mechanics of reproduction, his deep voice a low rumble. The bedroom lamp cast a warm glow over the scattered textbooks and printouts, illuminating your curious expression.

"Okay, so the male produces sperm, which are essentially cells with a tail..." He paused, realizing how bizarre that sounded. He pressed on. "Right. And the female ovulates an egg once a month. When the egg is fertilized by the sperm, it starts dividing and..." He flipped to a diagram showing the developing fetus week by week.

Your brow furrowed, your finger tracing the arc of a tiny spine. "But... how does the sperm get inside the egg?" you asked, genuinely puzzled.

He cleared his throat, feeling a bead of sweat trickle down his neck despite the coolness of the room. He'd been dreading this part. "Uh... well, that happens through... sexual intercourse."

You blinked at him, "What's that mean?"

Fuck. Think, choose your words carefully.

"It means... when a man and a woman... um... physically join... their genitals..." His face felt like it was on fire.

You tilted your head, studying him with frank curiosity. "You mean like... when you put your penis in a girl's vagina?"

Direct, to the point, no nonsense. Just like her.

He blinked. Twice. Thrice. "Y-yes. Exactly like that," he managed to croak out.

"And the penis is like a big, FAT sperm," you said, matter-of-factly. "So the sperm comes out of there and swims up to the egg when the girl ovulates, and then they meet and the egg gets fertilized."

He stared at you, momentarily lost for words. "Er... yes. More or less," he agreed weakly.

Jesus fucking christ.

"But... why do you think the penis has to go inside the vagina to do that? Why can't the sperm just swim through the girl's belly button or something?" You asked, genuinely puzzled.

Because it fucking feels incredible, that's why. Among other reasons.

"W-well, because..." He took a deep breath. "The male... releases the sperm directly into the female's reproductive tract... to increase the chances of fertilizing the egg," he said, trying to keep his voice level.

God, could he sound more like a robot?

You nodded slowly, considering this. "Oh. Okay. So... the penis goes inside the vagina, and then the sperm comes out and swims up to the egg. That's how a baby gets made."

You're oversimplifying it, but... yes. Basically.

"That's right," He confirmed, feeling like a fraud. He'd failed to mention the vast majority of the process – the hormones, the emotions, the raw, animalistic need that drove humans to couple.

At least until she's old enough to understand... and maybe hate me for it.

Looking down at the diagrams strewn across the bedspread, frowning slightly. "I still don't really get why the penis has to be inside the vagina though..." she mused. "Is that like... really important?"

Fuck me, it's not just important, it's essential. Indispensable. Irresistible.

And I really need to stop thinking about this before I embarrass myself.

He swallowed hard, trying to keep his voice even. "It's... yes. It's very important. For biological reasons," he said shortly.

Like the fact that a man loses his goddamn mind with lust when he's buried inside a woman's tight, wet heat. Fuck.

"Oh. Okay..." You said slowly. "I guess that makes sense."

Thank fuck for that.

Tapping your chin thoughtfully. "So... the penis gets hard, and then it goes inside the vagina, and then the man ejaculates the sperm, and that's how a baby happens..."

Too fucking right it is. Among other things.

"...and then the egg gets fertilized, and the baby starts growing in the womb..."

He nodded jerkily. "Yes, that's... that's pretty much it," he agreed, feeling like he was standing in the middle of a firing range with a live grenade in his hand.

And I'm the fucking grenade.

"And then the baby comes out and..."

And a man comes so fucking hard he sees stars, buried balls-deep in a woman's clenching, spasming cunt...

You were still talking, but your voice faded into static as a dizzying rush of images flooded his brain. The slick glide of a woman's hot, velvety walls gripping his aching cock like a fist, the filthy slap of skin on skin, the debauched sounds of pleasure spilling from kiss-swollen lips...

Fuck. Fuck. FUCK.

Step 2: Preparing to make a baby.

"So first you need to get comfortable, lie back on the bed." He instructs calmly, his deep voice low and clear. He watches as you reluctantly complies, easing herself onto the edge of the bed.

"Good girl," he praises softly, careful not to let his growing desire bleed into his tone. "Now spread your legs for me, nice and wide. I need to inspect you closely first."

You hesitate a moment before slowly parting your thighs, revealing your most intimate area to his hungry gaze. He feels his cock twitch in anticipation but forces himself to focus.

"Beautiful..." he murmurs, more to himself than to you. He kneels down between your spread legs, bringing his face level with your core. Inhaling deeply, he catches the scent - musky and heady, already tinged with arousal.

"The first step is to get you nice and excited," he explains, his voice still calm despite the building heat between them. "I'm going to start by stimulating your clit. Can you tell me where that is?"

When you glance down uncertainly, "Shh, it's okay. I'll guide you."

He parts the lower lips with his thumbs, exposing the delicate flesh of your inner walls. Your clit peeks out from beneath its hood, already glistening slightly.

"There it is," he murmurs, tracing the swollen nub with the pad of his thumb. "It's this sensitive little button here. When I touch it, you'll feel sparks of pleasure. Don't fight it."

True to his word, he begins to stroke your clit with a feather-light touch, circling and flicking the sensitive bundle of nerves. Almost immediately, you gasp and writhes beneath his ministrations.

"That's it sweetheart," he encourages, his own breathing growing a bit ragged. "Let yourself feel good. Get nice and wet for me..."

His fingers delve deeper, parting your slick folds and seeking the entrance to her channel. "You're already so wet," he groans softly, feeling her silky walls clench around his probing touch. "That's perfect..."

He works his fingers inside, curling them to brush against that spongey spot deep within, as his thumb continues to circle your clit. The dual stimulation has you arching off the bed, breath coming in short, sharp gasps.

"Good girl, just like that," he praises huskily, pumping his fingers steadily in and out of her tight heat. "You're doing so well, sweetheart. Getting so nice and ready for me..."

He leans in closer, his warm breath ghosting over your drenched folds. The scent of your desire is intoxicating, making his head swim. Unable to resist, he dips his head and runs his tongue along your slit.

"Mmm, you taste divine," he rumbles, his voice vibrating, "I could eat this pretty pussy for hours..."

He seals his lips around your clit and suckles gently. At the same time, he increases the speed and pressure of his fingers pumping into, curling them to ruthlessly stimulate that special spot inside.

You cry out sharply, hips bucking up against his mouth as your pleasure spirals rapidly. He just grips your thighs tighter, holding in place as he continues his relentless assault. Feeling your walls starting to quiver and clench erratically around his plunging fingers.

"That's it, baby," he urges between licks and suckles, his words slightly muffled. "Come for me. I want to feel you come all over my tongue..."

He redoubles his efforts, determined to bring you to the peak of ecstasy. His cock throbs almost painfully in his pants, leaking pre-cum at the thought of burying himself inside.

Crying out, back arching sharply as orgasm crashes over you. Inner muscles clench and spasm around his invading fingers, gushing fluid that he eagerly laps up.

As your spasms slowly subside, his tongue now lapping softly at your sensitive flesh, soothing through the aftershocks. He releases your clit from his lips and places tender kisses along the inner thighs as he slowly withdraws his fingers.

When he finally lifts his head, face is glistening with juices, a look of deep satisfaction on his handsome features. He crawls up your body to capture your lips in a deep, passionate kiss - letting you taste yourself on his tongue.

"Mmm, you're exquisite," he murmurs against her mouth when he finally comes up for air. "So responsive and sweet..."

Step 3: This is how a baby is made.

Taking a deep breath, his heart pounding in his chest as he gazes down at you, naked, flushed form. He can still taste you on his tongue.

"Now, sweetheart, the next step is for me to enter you," he explains, his voice low and rough with barely restrained desire. "I'll need to guide myself inside your tight little cunt. It might feel a bit intense at first."

His hands skim down your inner thighs, parting them further as he settles himself between them. With one hand, he frees his aching cock from the confines of his pants. It springs forth, thick and hard, the bulbous head already glistening with pre-cum.

Wrapping his fingers around the base, giving himself a few slow pumps as he lines himself up with your entrance. He can feel the slick folds fluttering against the tip of his member as he teases the opening. Biting his lip, he fights the urge to simply slam forward and bury himself to the hilt. He needs to go slow, to let her adjust to his size.

Slowly, he pushes forward, feeling tight walls parting for his girth. He has to grit his teeth at the exquisite sensation. You let out a shaky moan, fingers digging into the sheets below.

"That's it, baby," He grits out. "Take me inside you, feels so fucking good." He bottoms out with a low groan, heavy balls nestling against your ass. He stays still for a moment, letting you get used to the feeling of being so utterly stuffed full.

Leaning down, he capture your mouth in a searing kiss, swallowing whimpers and moans as he begins to move. He starts with shallow thrusts, withdrawing until just the tip remains inside, before plunging back in to the hilt. Gradually, he increases his pace, his hips rolling in a steady rhythm as he claims her body as his. He savors the taste of your mouth.

He breaks the kiss to trail his lips down the column of your throat, pausing to nip and suck at the racing pulse point. "You feel incredible," he murmurs, his voice rough with desire. "So tight and perfect, like you were made for me..."

His hand drifts down to your breast, cupping the soft mound possessively. He kneads it with gentle pressure, thumb brushing over the stiff peak of her nipple. Feeling it pebble further beneath his touch, he dips his head to take the hardened nub into his mouth.

You gasp sharply, arching up into him as he suckles the nipple, tongue flicking over the sensitive flesh.

"Gonna...fuck...cum inside you," he grunts, feeling his release fast approaching. "Gonna pump you full...make you...mine..."

His strokes become erratic, each driving thrust pushing him closer to the edge. He can feel his cock pulsing and jerking inside her slick sheath, his heavy balls drawing up in anticipation.

"Remember sweetheart," he pants harshly, eyes burning into you. "For me to fill you with my seed...you need to be ready to receive it. Open for me, baby...let me fill you up..."

He reaches down to rub tight circles over throbbing clit, wanting to feel you spasm around him as he finds his release deep inside. The lesson is simple - to make a baby, they both need to let go. Body tenses, muscles coiled taut as a bowstring as he teeters on the brink of ecstasy. With a hoarse shout of your name, he hilts inside one final time and erupts. His cock jerks and pulses, painting the insides white with his hot, thick seed.

"Fuuuuck, yes! Take it baby, take my cum!" eyes squeezing shut as wave after wave of intense pleasure crashes over him. Holding you in place as he empties himself deep inside.

Head thrown back and eyes rolled up as your own climax slams in your core. Milking him for every last drop of his potent release.

They remain locked together, chests heaving and sweat-slicked skin pressed close as they bask in the afterglow of their lovemaking.

"Mmm, you did so well, sweetheart," he murmurs after a long moment, brushing damp strands of hair from your face. "Took every drop like you were made for it."

He leans in to capture your lips in a slow, tender kiss.

"That's how you make a baby," he whispers.

[tipresearch123]

Impact of Single-Use Technologies on Cell Line Development

Single-use technologies have revolutionized the field of cell line development market, offering numerous benefits and impacting the entire process. These technologies, also known as disposable or flexible technologies, involve the use of single-use components and systems that eliminate the need for traditional stainless-steel equipment and cleaning validation procedures.

One of the major impacts of single-use technologies on cell line development is enhanced flexibility and speed. Traditional stainless-steel bioreactors require time-consuming cleaning and sterilization processes between batches, which can significantly slow down the development timeline. In contrast, single-use bioreactors can be quickly set up, used for a single production run, and then disposed of, allowing for faster turnaround times and increased productivity.

Moreover, single-use technologies offer improved contamination control. With traditional systems, the risk of cross-contamination between different production runs or cell lines is a concern. Single-use systems eliminate this risk, as each batch is produced in a completely new and sterile container. This ensures product integrity and reduces the need for extensive cleaning and validation procedures.

Another significant impact of single-use technologies is cost-effectiveness. Traditional stainless-steel equipment requires substantial upfront investment and ongoing maintenance costs. Single-use systems eliminate the need for capital-intensive infrastructure, equipment cleaning, and maintenance, resulting in reduced operational expenses. Additionally, single-use technologies minimize the risk of product loss due to equipment failures or contamination, further enhancing cost savings.

Furthermore, single-use technologies promote sustainability and environmental friendliness. Traditional stainless-steel equipment consumes significant amounts of water and energy during cleaning and sterilization processes. By eliminating these processes, single-use technologies reduce water usage, energy consumption, and overall carbon footprint, making them a more sustainable choice for cell line development.

Despite these numerous benefits, it is important to consider certain limitations of single-use technologies. For instance, scalability might be a concern when working with larger production volumes. Additionally, compatibility with certain cell lines or specific process requirements may require careful evaluation and customization of single-use systems.

Overall, the impact of single-use technologies on cell line development has been transformative. These technologies offer increased flexibility, improved contamination control, cost-effectiveness, sustainability, and accelerated timelines. As the field continues to advance, it is expected that single-use technologies will play an even more significant role in shaping the future of cell line development.

[marketreports-blog]

The global cell line development market size was exhibited at USD 6.51 billion in 2022 and is projected to hit around USD 17.52 billion by 2032, growing at a CAGR of 10.4% during the forecast period 2023 to 2032.

[mitalipingale]

https://tannda.net/read-blog/78747_stable-cell-line-development-market-share-overview-competitive-analysis-and-fore.html

The Stable Cell Line Development Market in 2023 is US$ 5.29 billion, and is expected to reach US$ 11.36 billion by 2031 at a CAGR of 10%.

[rahulp3]

Cell Line Development Market Outlook from 2023 to 2033

The Global Cell Line Development Industry is projected to experience consistent growth over the next decade, according to a recent industry analysis. Valued at US$3.01 billion in 2022, the market is expected to climb steadily at a CAGR of 4.1%, reaching an impressive US$4.67 billion by 2033.

The substantial growth of the Global Cell Line Development Industry is predominantly attributed to the increasing adoption of bio-therapeutics for treating chronic diseases like arthritis, diabetes, and cancer. This surge in demand is driving the need for automated cell line development and cell line development services.

Request a Sample of this Report Now!https://www.futuremarketinsights.com/reports/sample/rep-gb-7566

[chithereader]

l-o-v-e / aaron hotchner

part 2 to jealousy, jealousy!!!! word count: 2.1k pairing: aaron hotchner x f!bau!reader, shy!reader genre & cw: hotch being so in love!! jealousy plot, made-up case, and different use of cm character a/n: i got so much love for jealousy, jealousy it has been so surprising to me how much u guys loved it!! i really hope you enjoy this part 2 as we finally get some clarity to their feelings for each other!!

With your jaw slightly dropped, you manage to get out an “Uh..” Then you clear your throat as if that will make actual words come out then— “Uhh..” 

Now you didn’t know how long you were staring at Hotch. Yet somehow you were aware that the silence— your silence was stretching out for too long. Like a fish out of water, you continue to move your mouth soundlessly. 

And if you were actually underwater, you knew a series of air bubbles would come out in a line from your lips. 

Deep inside, Hotch was getting a little worried. That maybe he came on a little too strong. 

Was that too bold? Was that out of the blue? What if you think he’s joking? Or worse, what if you think he’s not taking you seriously? 

On the outside, Hotch is trying very hard to maintain his calm and collected composure, not letting too much emotion seep through his expression. Making sure he doesn’t look worried or too proud, too scared or too smug. 

The small smile on his face is one that he hopes to convey what he means: that he seriously likes you and that he doesn’t mean to embarrass you. But it is a smile that slowly fades the more he sees the panic growing in your eyes. 

Loud clapping shakes you both out of your individual worries as Derek teasingly cheers for the development in your romance— hooting and whooping about how the boss man has finally made his move. 

As if suddenly remembering that there are other people in the room, you both look around to check the other team members’ faces. 

To your surprise, Reid is blushing even more than you and Hotch are, and to absolutely no one’s surprise, Rossi is looking straight at Hotch with a smug grin. Raising his hands theatrically slow to clap painfully slow for his best friend. 

“My man.” Derek proudly says still clapping, and if he was brave enough to risk losing a hand tonight, he would have stood up to pat his boss on the back. 

Hotch shakes his head bashfully, cheeks turning increasingly red.  He looks down at his shoes to hide his face a bit, mumbling a low, “Shut up.” 

But Rossi being Rossi, was not gonna let the moment go, “I gotta say, Hotch, I didn’t think you’d ever do it…or anything actually.” 

Looking at Hotch, you start to giggle. He’s got his head facing the ceiling, acting playfully exasperated at his team’s antics. No doubt already regretting his public expression a little bit. 

But the laughter dies down into soft giggles, and he straightens a little to look at you. Catching your eye, you smile back at him softly, also hoping that he’d understand what you’re saying with that little smile. I like you too. Don’t worry, you didn’t embarrass me. 

Hotch’s worries are instantly quieted by your smile. Like dust settling on the ocean floor, he feels at peace. 

Your little staring moment though, is suddenly interrupted by his cell ringing. And the room’s mood sombers knowing that there can only be one reason someone will call one of your cells late at night— a new body was just found. 

—

It’s 7:00am and the sun has risen brightly. Reid and Rossi went to the ME with the body to further examine what’s been done. Meanwhile you, Morgan, Seaver and Hotch stay behind at the crime scene, knowing a fresh scene can tell you the most right now.

You’ve been staying close to Morgan as he theorizes the unsub’s movements. Following and coming up with theories of your own in terms of the order of the unsub’s entry and exit. 

But as much as you are focused on the case, you look at Hotch and Seaver every now and then, who are interviewing witnesses and authorities on the side. 

Hotch catches you looking at him and Seaver, just as Seaver holds on to his arm to fix the strap of her heel. You may have looked extra irritated but you’d blame it on the sun being on your face. 

Looking back at Derek who had gotten quiet, you find him smiling at you teasingly. Already aware of what you were just looking at— more like who. You roll your eyes at him, “Shut up, chocolate.”

Derek shakes his head as he laughs, taking his sunglasses from where it hung his shirt and wears it on you. 

“Calm down, Cyclops. You might just kill the two of them if you’re not careful.” 

You gasp at his audacity, watching his back as he walks away, not even giving you the chance to respond to his teasing. 

Not wanting to stare at his back any longer, you turn around to pick up right where you left off. Only to have the fright of your life seeing Hotch right in front of you. 

With a hand on your chest you catch your breath, “Oh my god! How even—“ One second he’s more than 6 feet away from you, the next second he’s not even 4 inches from you. 

Your heart beats even faster as Hotch’s hands reach up to your face to remove Derek’s sunglasses. “Morgan!”, he shouts and tosses Derek his glasses. 

Derek catches it instinctively and looks to the both of you in confusion, but Hotch looks back at you and takes his own sunglasses off his face to wear it on you. 

Seaver watches all of this unfold from behind Hotch, and you could see it annoyed her. But she puffs her chest and turns to the people she and Hotch were talking to, continuing the interviews they were conducting. 

— 

Now during the case, obviously there wasn’t really any time for you and Hotch to discuss the romance brewing between the two of you. Absolutely no time to indulge in personal matters at a time where other people’s lives depended on you. 

But that’s not to say that Hotch has not followed up his advances with more actions. Not at all— the complete opposite actually. 

He has only become increasingly affectionate and bolder with his actions. He seems to have given up on holding himself back around you. He’s constantly sitting beside you, placing his hand on your lower back as you walk, then he stands behind you constantly towering over you whenever, wherever. 

He’s even given you his handkerchief multiple times so you could wipe your sweat, and when you guys ordered takeout for the night, he made sure to unpack yours for you and hand you your utensils, even standing to get you water from the pantry before he even touches his food. 

He’s been crazy sweet and even more protective than usual, you almost didn’t need words to confirm how he feels about you… if it weren’t for Seaver who has also gotten bolder with her advances towards Hotch. Then I mean, maybe a little reassurance would be nice. 

It seems as if the recent development in yours and Hotch’s romance was something Seaver saw as a challenge- a hurdle she has to get over to win Hotch. 

Annoying you even more, when she arrived at the precinct the next day wearing a revealing top and tight pencil skirt. She looked good, you had to admit. 

Looking down at your own attire, with jeans, boots, and a plain shirt. You felt a little defeated. Obviously you weren’t going to attract Hotch being this plain. 

But you also wanted to be ready. The team was closing in on the unsub who has become more and more erratic, you could almost predict a chase and maybe even a tussle. 

You were standing beside Reid, looking at the board trying to uncover a pattern in the unsub’s dump sites when you heard an agitating little voice say, “Hotch, I think my top unbuttoned at the back. Could you get it for me?”

Tension instantly brews. The team, who has caught on to Seaver’s ploy early on, awaits your reaction. You could feel their gazes on your back, even from Reid who you could feel checking on you from the corner of his eye from where he stands to your right.

But you refuse to give in. You continue- more like pretend to- analyze the map on the board. Even tilting your head a little to sell that you’re really not paying attention to the two. However in all honesty, all your other senses are very much attuned to whatever’s happening behind you. 

Rossi cleared his throat, making you check the room’s reflection on the window on your left through the corner of your eye. And you watch as Seaver turns in her seat away from Hotch, anticipating him leaning close and putting his hands on her. 

Now you thought that since Hotch had an idea about how Seaver makes you feel, that he’d keep his distance. You know, set those boundaries to appease you. But to your surprise, Hotch leans over the distance between his chair and hers, and reaches over to button her top. 

You could feel your face heating up. You don’t know if he simply didn’t care, if he was oblivious, or if he did it on purpose. But now was not the time to act up and make a big deal out of something so trivial. You were all so close to catching the unsub, you poured your focus on the case instead. 

But you need a moment to yourself, maybe a little fresh air or even a pep talk in the bathroom mirror will do. Just as you were about to excuse yourself stepping back from the board, you hear Hotch close the file he was reading- before he was interrupted- loudly. 

His stern voice soon follows, “Just a little advice, Agent Seaver: if you’re assigned to the field, dress like it. Then you wouldn’t have to worry about buttons popping or heels snapping while you’re chasing an unsub or racing to save someone’s life.” 

You couldn’t stop yourself even if you wanted to. Their reflection on the window was blurry enough that you couldn’t make out their facial expressions, so when you hear Hotch’s stern voice your head snaps to look at him in surprise, not expecting him to be annoyed at Seaver given that he’d just helped her. 

You almost feel bad for Seaver who’s turning red in embarrassment. She’d obviously put together an outfit for Hotch. You all knew she was an outstanding agent, so to jeopardise her performance for a man’s attention seemed weird even for her. 

–

To your surprise, her advances towards Hotch did not stop even after his dig at her unprofessionalism. 

As you were all boarding a jet well into the night, exhausted from the long case, you all noticed Seaver subtly rushing to sit first. Unsurprisingly, she chose the seat beside Hotch’s usual seat. Acting normally, she pulled out a blanket settling in her seat. 

But Hotch, who has been behind you the whole time, was just shadowing your movements. The most exhausted out of all of you, he wasn’t even thinking about where he’ll sit. He was blindly following you like a puppy, with a hand on your waist as if to not get lost. 

He was actually just waiting for you to sit somewhere, then he’d sit beside you. So you chose a couple-seat on the far end of the jet, away from Seaver. Neither of you have the energy to deal with her antics. 

In a last attempt to get Hotch to her, Seaver calls out “Hotch, I saved you your seat!”, even opening up her arms that are covered by the blanket as if to invite him to her warmth. 

But Hotch only looks at her silently, blinking. Then in less than 10 seconds, Hotch takes your hand, intertwines it with his, kisses yours softly, and crosses his arms as he closes his eyes to sleep- leaving your hand somewhat trapped to his body. 

You’re surprised at the bluntness of his affection, considering most of your team members were looking at you after Seaver called him out. 

Stealing a glance at Seaver, you catch her shoulders drop before she settles back into her seat, while Morgan mouths to you “Told you,” from across her. 

Turning your head to look at Hotch, you can tell he isn’t asleep yet though his eyes are closed. You squeeze the hand intertwined with yours, trying to get it out of his grip and crossed arms– he opens his eyes to look at you and softly whines, “Stoopp.”

“Hey!” you whisper. 

He breathes out a grumpy, “What?” to which you smile softly and say, “Fine. I guess I’m your girl.” 

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