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HighPrep PCR Beads vs. AMPure XP: 3 Reasons to Make the Switch

When it comes to PCR cleanup in next-generation sequencing (NGS) workflows, SPRI bead-based purification remains the gold standard. For years, AMPure XP has dominated the market with its reliable but expensive solution. However, labs are now increasingly turning to a smarter alternative—MagBio Genomics' HighPrep PCR beads.

Why are more researchers switching from AMPure XP to HighPrep PCR? Because they want better value, equal (or better) performance, and true workflow flexibility. In this article, we dive into the top three reasons why HighPrep PCR has become the preferred choice for DNA purification—and why it might be time for your lab to make the switch.

Reason 1: Cost-Efficiency Without Performance Compromise

One of the biggest drivers for labs to reconsider their reagent suppliers is cost. AMPure XP is known for high recovery rates and reproducibility, but its cost per reaction adds up fast—especially for high-throughput facilities. HighPrep PCR offers a cost savings of up to 40% per sample, without compromising yield or quality.

Side-by-side tests show:

DNA recovery from 100 bp to 2 kb is nearly identical for both products.

HighPrep PCR performs equally well in removing primers, dimers, and enzymes.

Labs switching to HighPrep PCR report equivalent sequencing coverage and fidelity.

Over the course of a year, switching to HighPrep PCR can lead to tens of thousands of dollars in savings for mid-to-large-scale labs. For academic labs, that translates into more samples processed, more grant deliverables achieved, and more experiments completed on time.

By reducing per-sample purification costs, labs using HighPrep PCR can reallocate budget toward additional experiments, new equipment, or expanded sample sizes.

Reason 2: Reliable, Consistent Performance Across Applications

Whether you're doing amplicon sequencing, library preparation, or qPCR cleanup, consistency matters. HighPrep PCR beads are manufactured under strict quality controls to ensure batch-to-batch reproducibility. Unlike some generic alternatives, HighPrep PCR has gone through rigorous testing to meet the needs of sensitive molecular biology workflows.

HighPrep PCR is validated for:

Illumina and Ion Torrent library prep workflows

Post-PCR cleanup for qPCR and Sanger sequencing

Adapter dimer cleanup after ligation

Double-sided size selection protocols for fragment tuning

Automation with KingFisher, Tecan, and Hamilton platforms

In fact, one high-throughput genomics core saw a 10% improvement in recovery reproducibility after switching from AMPure XP to HighPrep PCR. The magnetic bead suspension in HighPrep remains uniform and pipetting-friendly, reducing technical variability between users and across runs.

For long-term experiments that demand reproducible input DNA, this level of consistency translates directly into higher confidence in downstream data.

Reason 3: Flexible Workflows and Automation Compatibility

HighPrep PCR is designed with user flexibility in mind. From low-input DNA to automation integration, it supports a range of protocols without sacrificing ease of use. It’s fully compatible with manual and automated workflows, making it suitable for both bench-top researchers and large core labs.

Key advantages include:

Stable bead suspension compatible with multichannel pipettes and robotic arms

Broad input range from 5 ng to several μg of DNA

Works in 96- and 384-well formats with minimal protocol adjustments

Validated for double-SPRI workflows with tunable size exclusion parameters

Automation labs save not only on cost, but also reduce hands-on time and error rates, thanks to HighPrep's compatibility with widely used platforms like KingFisher Flex and Tecan Fluent.

How HighPrep PCR Matches Up in Common Use Cases

Let’s explore how HighPrep PCR performs in real lab scenarios:

Use Case: NGS Library Prep for Illumina

Objective: Cleanup post-PCR and remove dimers before sequencing.

Outcome: HighPrep PCR shows identical Bioanalyzer traces compared to AMPure XP, with better yield retention in low-input samples.

Use Case: Size Selection for Targeted Panels

Objective: Enrich for 350–500 bp fragments.

Outcome: Dual-SPRI using 0.6x/0.8x ratio yields high purity with narrow distribution, matching performance of AMPure XP.

Use Case: High-Throughput PCR Cleanup in 384-Well Plates

Objective: Maximize speed and recovery.

Outcome: HighPrep PCR allows faster separation and easier elution, increasing daily throughput by 20%.

Performance Comparison: HighPrep PCR vs AMPure XP

Why AMPure XP Users Are Making the Switch

While AMPure XP has long set the standard, users report growing frustration with:

Rising reagent costs

Supply chain delays and backorders

Limited flexibility in size selection

Reduced support for protocol customization

MagBio Genomics recognized these pain points and engineered HighPrep PCR to offer a more responsive, transparent, and scalable alternative. Labs making the switch often report that they "should have done it sooner."

Technical Tips for Optimizing HighPrep PCR Use

Use fresh ethanol for wash steps to minimize salt retention.

Avoid overdrying the beads, which can reduce elution efficiency.

Use magnetic stands with tight separation windows for fast and clean separation.

Pre-wet pipette tips to reduce bead carryover during transfers.

Elute in a pre-warmed buffer (37°C) for maximum recovery from HMW DNA.

MagBio also provides detailed protocol templates for Illumina workflows, long-read prep, and automated systems, ensuring a smooth transition from AMPure XP.

Transition Made Easy: From AMPure XP to HighPrep PCR

MagBio offers personalized onboarding and protocol migration support. For labs making the switch, MagBio provides:

Side-by-side comparison guides

Bead ratio conversion charts

Automation scripts for major platforms

Free sample kits for initial validation

This ensures a seamless transition with minimal disruption and quick results.

Frequently Asked Questions

Q: Will I need to change my current protocol?  A: In most cases, no. HighPrep PCR can be used with the same volumes and timings as AMPure XP, or optimized for specific workflows.

Q: Can I still do double-sided size selection?  A: Yes, HighPrep PCR is fully compatible with SPRI-based dual-cut protocols.

Q: Is there a minimum or maximum DNA input range?  A: HighPrep PCR works with inputs from 5 ng to over 5 μg of DNA.

Q: What about RNA applications?  A: While this kit is designed for DNA, MagBio offers HighPrep RNA kits specifically for RNA cleanup.

Final Thoughts

Switching to HighPrep PCR from AMPure XP isn't just about saving money—it's about empowering your lab to do more, faster, and with greater flexibility. With equivalent performance, lower cost, and validated automation support, HighPrep PCR delivers everything modern genomics labs need.

Labs around the world are making the switch. From academic research centers to clinical sequencing facilities, HighPrep PCR is transforming how DNA cleanup is done—one sample at a time.

Make the switch today and join hundreds of labs improving their workflows with HighPrep PCR beads. Explore the HighPrep PCR Clean-Up Kit at MagBio Genomics Inc.

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3 Reasons to Switch from AMPure XP to HighPrep PCR Beads Today

If you’re working in genomics, you’ve almost certainly used AMPure XP beads. They’ve become a staple in next-generation sequencing (NGS) library prep and PCR cleanup. But what if there were a more affordable alternative that performs just as well — or better?

MagBio Genomics’ HighPrep™ PCR Clean-Up Kit.

As labs face rising costs, supply chain issues, and increasing demand for automation-compatible solutions, more researchers are making the switch. In this article, we break down the top three reasons why switching from AMPure XP to HighPrep PCR beads could be the smartest move your lab makes this year.

1. Significantly Lower Cost Without Sacrificing Quality

Let’s face it: AMPure XP beads are pricey. While they offer high-quality results, many labs — especially high-throughput and grant-funded ones — struggle to justify the cost.

HighPrep PCR beads provide:

Up to 40% savings per reaction

Equivalent or better DNA recovery (90%+ for 100 bp – 10 kb)

Full compatibility with AMPure XP protocols

You get AMPure XP performance at a fraction of the price.

Real-World Example:

One genomics core lab reported that switching to HighPrep PCR saved over $4,000 per month, allowing them to reinvest in sequencing reagents.

2. Drop-In Replacement with Seamless Protocol Compatibility

Switching purification kits can feel risky. What if the new product doesn’t work with your established workflow?

Not with HighPrep PCR.

Uses the same SPRI-based magnetic bead chemistry as AMPure XP

Compatible with standard bead-to-sample ratios (0.6x, 1.0x, 1.8x)

Supports applications from PCR cleanup to size selection

Works with both manual and automated systems (KingFisher, Hamilton, Tecan)

Whether you're cleaning up amplicons or prepping an NGS library, HighPrep PCR fits right in.

Bonus: No training curve for your staff.

If your team knows AMPure XP, they already know how to use HighPrep PCR.

3. Proven Performance for PCR Cleanup and NGS Library Prep

HighPrep PCR beads aren’t just a budget option — they’re a high-performance tool used in top labs worldwide.

Performance Highlights:

Consistent DNA recovery rates across multiple sample types

Effective removal of primers, nucleotides, enzymes, and buffers

Precise size selection for fragments from 100 bp to 1 kb+

Excellent results in qPCR, Sanger sequencing, and NGS workflows

What Researchers Are Saying:

"We validated HighPrep PCR beads side-by-side with AMPure XP and saw no difference in yield or sequencing quality. But we cut our costs nearly in half." — Director, Clinical Genomics Lab

"The automation compatibility and lot-to-lot consistency sealed the deal for us. HighPrep PCR is now our default bead cleanup kit." — Research Scientist, Cancer Center

Bonus: Reliable Availability and Sustainable Packaging

Unlike some suppliers, MagBio Genomics has ensured robust supply chains, with flexible packaging options and bulk volumes.

Reduce plastic waste with column-free, SPRI bead format

Get shipments fast and consistently

Choose from 5 mL to bulk liter sizes

With HighPrep PCR, you won't miss a project deadline due to backorders or delays.

Conclusion

AMPure XP has long been the gold standard in DNA purification. But in today's research environment, efficiency, cost control, and flexibility are just as important as performance.

MagBio Genomics' HighPrep PCR Clean-Up Kit delivers:

Trusted SPRI bead chemistry

Significant cost savings

Seamless compatibility with existing workflows

Proven results in high-throughput and clinical labs

Whether you're managing a university core facility or scaling a biotech startup, the time to make the switch is now.

Ready to make a smarter switch?

Shop the HighPrep™ PCR Clean-Up Kit and experience why labs around the world are choosing it over AMPure XP.

[articlepublication]

SPRI Bead-Based Purification: Optimizing PCR Cleanup for Superior NGS Results

In molecular biology, precision and purity are everything. Whether you’re prepping for next-generation sequencing (NGS), cloning, or qPCR, the quality of your DNA directly influences your results. PCR cleanup is one of those essential steps that can make or break your downstream applications. And when it comes to PCR cleanup, Solid Phase Reversible Immobilization (SPRI) bead-based purification is the method of choice for researchers worldwide.

SPRI bead-based methods combine efficiency, scalability, and versatility in one simple workflow. They’ve become indispensable for high-throughput labs, small academic teams, and commercial genomic facilities alike. In this article, we’ll explore how SPRI beads work, best practices for optimizing PCR cleanup, and how cost-effective solutions like MagBio Genomics’ HighPrep™ PCR Clean-Up Kit are transforming the purification landscape.

What is SPRI Technology?

SPRI stands for Solid Phase Reversible Immobilization. This method uses paramagnetic beads that bind nucleic acids in the presence of polyethylene glycol (PEG) and salt. The DNA binds to the beads, while unwanted components like salts, primers, enzymes, and nucleotides remain in the solution.

Key benefits include:

Size-selective binding

High recovery rates for DNA fragments ranging from 100 bp to >10 kb

Simple workflows that replace spin-column protocols

Automation-friendly—ideal for 96- and 384-well plates

The Role of PCR Cleanup in NGS Workflows

NGS library preparation involves several steps that require precise DNA handling. After amplification, it’s critical to remove unwanted materials such as leftover primers, free nucleotides, and enzymes. These contaminants can:

Inhibit adapter ligation

Interfere with quantification and normalization

Reduce overall sequencing quality

SPRI beads simplify this process. Add the beads, bind the DNA, wash away the contaminants, and elute clean DNA that’s ready for the next step.

Key Advantages of SPRI-Based PCR Cleanup

1. Efficiency

SPRI workflows reduce the number of steps and eliminate centrifugation. This makes them ideal for high-throughput pipelines and minimizes user error.

2. Scalability

From a handful of samples to thousands, SPRI bead protocols are easily scaled and automation-compatible.

3. Customizability

You can fine-tune your purification with bead-to-sample ratios:

1.8x for total DNA recovery

0.6x to exclude smaller fragments (<300 bp)

0.8x to retain larger DNA for long-read workflows

How to Optimize SPRI Bead-Based PCR Cleanup

Choose the Right Bead-to-Sample Ratio

This is perhaps the most important variable. Use:

1.8x for complete fragment retention

0.8x to remove primer dimers and small artifacts

Avoid Overdrying the Beads

After ethanol washing, beads should be air-dried until no visible liquid remains. Overdrying can make it difficult to elute DNA and decrease recovery.

Use Fresh Ethanol

Old or cold ethanol can decrease the wash efficiency and lead to poor DNA purity.

Elute in the Right Volume

Using 20–50 μL of elution buffer ensures efficient recovery while keeping the DNA concentration high enough for downstream use.

Common Applications in Molecular Biology

SPRI beads aren’t just for NGS. Their versatility spans across:

PCR cleanup: Removing enzymes, primers, dNTPs

Cloning: Preparing inserts for ligation

Sanger sequencing: Enhancing read clarity by removing noise

qPCR: Ensuring template purity for quantification

cDNA synthesis and RNA-seq: Purifying double-stranded cDNA

HighPrep™ PCR Clean-Up Kit: A Reliable SPRI-Based Solution

MagBio Genomics’ HighPrep™ PCR Clean-Up Kit is designed to deliver all the benefits of SPRI bead-based purification at a more accessible cost.

Key Features:

Compatible with all major NGS platforms

Comparable or better recovery than AMPure XP

Validated for manual and automated protocols

Stable shelf life and consistent batch quality

HighPrep™ beads use the same SPRI chemistry as traditional solutions but come at a fraction of the cost, making them perfect for cost-sensitive labs without sacrificing performance.

Automation and High-Throughput Compatibility

As research scales up, automation becomes essential. HighPrep™ PCR Clean-Up is compatible with liquid-handling platforms like:

Thermo Fisher KingFisher Flex

Hamilton Microlab STAR

Tecan Fluent and Evo

These integrations allow genomic core facilities to streamline operations and reduce sample-to-sample variation.

Testimonials and Case Studies

“Switching to HighPrep™ not only cut our cleanup costs in half, but also gave us flexibility with automation. We saw no drop in yield or data quality.” — Senior Scientist, Biotech Startup

“We routinely process 10,000 samples a month, and HighPrep™ has been instrumental in keeping our workflow smooth and cost-effective.” — Genomics Core Facility, UK

Sustainability and Supply Chain Reliability

Unlike spin columns, SPRI beads reduce plastic usage. HighPrep™ PCR Clean-Up is also offered in bulk formats to minimize packaging waste. And unlike some competitors, MagBio ensures consistent availability with a stable supply chain.

Cost Comparison

When to Use SPRI-Based Purification

SPRI bead cleanup is ideal when you need:

Fast and reproducible DNA purification

High throughput compatibility

Flexible size selection for fragment trimming or retention

Reliable performance for sensitive applications like NGS

Final Thoughts

SPRI bead-based purification has changed the game for PCR cleanup and beyond. The simplicity, accuracy, and scalability of this approach make it indispensable in modern molecular biology. With cost-effective and high-performing kits like HighPrep™ PCR Clean-Up from MagBio Genomics, it’s easier than ever to streamline your workflow, reduce costs, and get consistent, high-quality results.

Upgrade Your PCR Cleanup Workflow

Explore the power of SPRI-based purification with the HighPrep™ PCR Clean-Up Kit. It’s the perfect solution for efficient, cost-effective DNA purification in any lab setting.

[articlepublication]

Best Custom Snap-On Veneers in the USA: How to Get a Perfect Smile at Home

A beautiful smile can do wonders for your confidence. However, not everyone can afford expensive dental procedures like veneers or implants. That’s where custom snap-on veneers come in. These affordable, non-invasive dental covers provide an instant transformation, helping you achieve a flawless smile from the comfort of your home.

In this guide, we’ll explore the best custom snap-on veneers available in the USA, their benefits, and how you can get the perfect fit for your teeth without stepping into a dental office.

What Are Custom Snap-On Veneers?

Custom snap-on veneers are removable dental appliances that fit over your natural teeth to enhance their appearance. Unlike traditional veneers, which require permanent bonding, snap-on veneers provide a temporary and affordable solution to cover gaps, stains, chips, or missing teeth.

Benefits of Custom Snap-On Veneers

Affordable Alternative to Traditional Veneers – Snap-on veneers cost significantly less than permanent dental veneers.

Non-Invasive and Painless – No drilling, injections, or enamel removal required.

Instant Smile Makeover – Get a perfect smile in days instead of waiting weeks for traditional veneers.

Removable and Convenient – Wear them when needed and remove them easily for cleaning or sleeping.

Custom Fit for Comfort – Designed using dental impressions to ensure a natural and snug fit.

How to Choose the Best Custom Snap-On Veneers in the USA

To get the best results, it’s essential to choose high-quality snap-on veneers that offer durability and a natural appearance. Here are key factors to consider:

1. Customization and Fit

Look for brands that provide a dental impression kit to create custom-fit veneers tailored to your teeth.

2. Material Quality

Choose veneers made from high-quality dental-grade resin to ensure durability and a natural look.

3. Realistic Appearance

Opt for veneers available in multiple shades to match your natural teeth color.

4. Usability While Eating

Some custom veneers allow you to eat and drink comfortably, while others are meant for cosmetic use only. Select one based on your needs.

5. Customer Reviews and Testimonials

Read reviews and testimonials to understand other users’ experiences and ensure product reliability.

Top Brands for Custom Snap-On Veneers in the USA

1. Removable Veneers USA

A trusted name in the industry, Removable Veneers USA offers high-quality, custom-made snap-on veneers that provide a natural look and comfortable fit.

2. Instasmile

Known for affordability and quick processing, Instasmile offers various veneer options suitable for different needs.

3. TruSmile Veneers

TruSmile provides durable, stain-resistant veneers customized using dental impressions for a seamless fit.

How to Order Custom Snap-On Veneers Online

Choose a Reputable Brand – Research and select a trusted provider.

Order an Impression Kit – Most companies send a kit for taking an accurate mold of your teeth.

Send Back Your Impression – Follow the provided instructions to create and return your mold.

Wait for Custom Production – Your veneers will be crafted based on your unique dental structure.

Enjoy Your New Smile – Once received, simply snap them on and enjoy your transformed smile!

Caring for Your Custom Snap-On Veneers

Clean Daily – Use a soft brush and gentle cleaner to remove debris and stains.

Avoid Hard Foods – If your veneers are not designed for eating, avoid chewing hard foods.

Store Safely – Keep them in a protective case when not in use.

Follow Manufacturer Guidelines – Each brand may have specific care instructions to extend their lifespan.

Final Thoughts

Custom snap-on veneers provide an easy and affordable way to achieve a stunning smile without the commitment or cost of permanent dental work. Whether you’re looking for a temporary cosmetic fix or a long-term solution, high-quality veneers can help you regain confidence in your smile.

If you’re ready to transform your teeth, explore your options and invest in the best custom snap-on veneers today! Visit Removable Veneers USA to get started on your journey to a flawless smile.

[articlepublication]

MagBio Genomics’ Kits Simplify DNA Normalization in Next-Generation Sequencing

In modern genomics research, ensuring accurate and consistent DNA input is crucial for obtaining high-quality sequencing data. One of the most important steps in achieving this is DNA normalization, a process that ensures uniform DNA concentration across samples. With the increasing demand for precision in Next-Generation Sequencing (NGS), having a reliable method for DNA normalization is essential.

What is DNA Normalization and Why is it Important?

DNA normalization is the process of adjusting the concentration of DNA samples to a uniform level before sequencing or downstream applications. Unequal DNA input can lead to inconsistent sequencing coverage, impacting data quality and increasing variability in results. Effective normalization helps to:

Improve sequencing accuracy by maintaining uniform representation of all samples.

Reduce sample-to-sample variability, leading to more reliable results.

Optimize reagent usage, preventing waste and lowering costs.

Streamline workflows, making high-throughput sequencing more efficient.

Challenges in DNA Normalization

Traditional DNA normalization methods include spectrophotometry (NanoDrop), fluorometry (Qubit), and automated liquid handling. However, these approaches come with challenges:

Time-consuming and labor-intensive: Manual normalization can be tedious and error-prone.

Inconsistent measurements: Variability in DNA quantification methods can lead to inaccurate normalization.

High reagent costs: Some normalization methods require expensive reagents and equipment.

Given these challenges, researchers need an efficient, reproducible, and cost-effective solution.

MagBio’s DNA Normalization Kits: A Game-Changer for Genomic Research

MagBio Genomics has developed innovative DNA normalization kits that address the limitations of traditional methods. These kits utilize magnetic bead-based technology, ensuring accurate, scalable, and high-throughput DNA normalization for NGS and other applications.

Key Benefits of MagBio’s DNA Normalization Kits

Automated and Scalable: Ideal for high-throughput laboratories, reducing manual errors and hands-on time.

Highly Reproducible: Consistently delivers accurate DNA concentrations for improved sequencing performance.

Cost-Effective: Reduces reagent waste and the need for additional quantification steps.

Easy Integration: Compatible with liquid handling systems and standard laboratory workflows.

Non-Damaging to DNA: Ensures high-quality DNA with no degradation, making it suitable for sensitive applications like long-read sequencing.

How MagBio’s DNA Normalization Kits Work

MagBio’s kits utilize paramagnetic beads that selectively bind DNA molecules, allowing for precise concentration adjustments. The process involves:

Binding DNA to magnetic beads in a controlled manner.

Washing Steps to remove excess DNA and contaminants.

Elution in a defined volume, yielding normalized DNA ready for sequencing.

This simple, robust method eliminates the need for manual quantification, making it an efficient alternative to conventional normalization approaches.

Applications in Next-Generation Sequencing (NGS)

NGS platforms require uniform DNA input for optimal sequencing results. MagBio’s DNA normalization kits are designed to meet the needs of various sequencing applications, including:

Whole Genome Sequencing (WGS)

RNA-Seq and Transcriptomics

Targeted Sequencing Panels

Metagenomics and Microbiome Research

Amplicon-Based Sequencing

By ensuring consistent DNA input, researchers can achieve more accurate sequencing coverage, reducing variability and improving downstream data analysis.

Why Choose MagBio for DNA Normalization?

MagBio Genomics has a strong reputation for delivering high-quality magnetic bead-based solutions for nucleic acid purification and sequencing. Their DNA normalization kits are trusted by genomics researchers worldwide for their ease of use, efficiency, and reliability.

Whether you are running a small-scale study or handling high-throughput sequencing projects, MagBio’s DNA normalization kits provide the consistency and accuracy needed to optimize your workflows.

Conclusion

DNA normalization is a crucial step in NGS workflows, ensuring reproducibility and accuracy in sequencing results. MagBio’s DNA normalization kits offer a cutting-edge solution that simplifies the process, improves efficiency, and enhances data quality. By integrating these kits into your lab, you can streamline sequencing workflows while maintaining high standards of performance.

[articlepublication]

Looking to improve NGS Sample Prep?

NGS (Next-Generation Sequencing) has aided rapid strides in genomics data generation but getting to the results may prove labor and time-consuming. Sample prep can be complicated if samples are damaged or degraded. However, experts recommend the following tips to accelerate and simplify the sample prep.

Accept that you will hit bottlenecks

A sequence may have a natural variation or the variation may result from the NGS workflow. There are different steps in sample prep and any of them could be responsible for variation, for example, inaccurate pipetting can impact data. After a long NGS process, if you find that you have bad data, you would have to start again. This means more sample requirements as well as added reagent and labor costs. This is why NGS sample prep needs to be consistent and repeatable.

Look for NGS Sample Prep Tools

Find the right NGS sample prep products for your research so that you can eliminate the external sources of variability and inconsistency in your workflow, and can be more assured of true results.

Generally, the biggest challenges in NGS sample prep include:

throughput

many sample-prep steps

hands-on time

human error

cost per sample

cooling of samples and reagents

finding the right kit for a specific application

Specific challenges for sample prep upstream of long-read sequencing include extracting high molecular weight DNA quickly and easily. The most widely used Phenol extraction is inconvenient and labor-intensive.

Handling high molecular weight DNA is complex. It does not mix homogeneously into the solution easily and is likely to yield inaccurate quantitation and purity assessment. But some applications need longer DNA fragments. Hence, it is important to use high-quality input material, as well as an extraction workflow that keeps nuclease activity and DNA shearing to a minimum.

How to fix it?

Automation is essential for standardizing complex NGS sample-preparation workflows. Compared to manual pipetting, liquid-handling workstations provide more reproducibility and accuracy. Many automated workstations support multiple library protocols for added flexibility.

Automation can be added in various ways –

semi-automation in nucleic-acid extraction, clean-up steps, size selection, adapter ligation, enrichment with an automated liquid-handling solution

full automation for the execution of the whole library prep workflow

If you are looking for high-quality magnetic bead-based kits for nucleic acid isolation and sample collection and stabilization tubes, call one of the most trusted RNA extraction kit manufacturers in the USA, MagBio Genomics at (301) 302-0144.

[articlepublication]

The Making of a Successful Fishing Trip

Fishing is more than just a sport. For many anglers, it is an escape from the stresses of daily life. However, planning and making arrangements for one such trip can itself be stressful, ruining the experience. Dupe A Fish can help you plan a great fishing trip for you and your friends, matching your needs. Here are some more tips to help you plan and enjoy a successful, fun fishing trip minus the stress.

Learn about the location

Having a fair idea about your location can help you have a better time. For instance, if you are going on fishing boat trips in California, consider the following factors about your destination:

What are the likely weather conditions at the time of your trip? Is it going to be cold? Rainy? Warm? Windy? Answers to these questions can help you prepare for the trip.

Which fish species are you targeting? Which species are available? What are the best fishing spots? Finding the answer to such questions can help you get the right equipment, fish baits, and more.

Look for fishing guides in the area.

Look for budget-friendly accommodation.

What is the best time to go fishing throughout the day? Where are the best spots for catching the species of your choice?

Check the water levels and temperature before you go.

Hiring a fishing guide

You can enhance your fishing experience with guided fishing trips on the Green River in Utah. Hiring a guide can enhance your trip as these people usually have years of experience and knowledge. They can take you to the best fishing spots and teach you new fishing tricks. Not just that, you also get to learn and gain more fishing knowledge.

Carry the right gear

Bringing the right fishing gear with you is essential. Learning about the weather conditions and other factors in advance helps. If the climate is going to be warm or sunny, make sure to carry sunscreen and sunglasses. For cold and rainy weather, pack your warm clothes and a raincoat. In addition to that, bring an extra rod with you for backup. Make sure to bring suitable fish baits for different species. Anglers often cut their fingers or hands while working with the hooks. Don’t forget to carry a first aid kit for emergencies. If you are going to be out long, make sure to stay hydrated. A mini water cooler can keep your drinks cool, especially on a hot day.

Know the rules and regulations

As a responsible angler, it is your duty to get a valid fishing license for the location and follow the local rules and regulations. Many areas have catch limits, catch and release laws, size limits, or other rules. Make sure you are aware of them before heading to the waters for fishing.

Dupe A Fish can help arrange guided fishing trips on the green river, California, Alaska, and many more destinations.

Author: Christian Bacasa

Dupe a Fish, LLC.

Find a trip that is perfect for you or your group. We are working to have the widest variety of guides and services available on the web and specialize in offering trips that are perfect for you, your group, and your budget. Start defining your perfect trip now. We make it easy!

Connect with us on Social Media

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Want to learn more about fly fishing? Subscribe to the Fly Fishing Insider Podcast

[articlepublication]

Places in the US You Must Visit at Least Once For Fishing

America offers a great many places for fishing. Over the years, the fishing enthusiast community has increased tremendously. Whether you are an avid sportsman or someone looking to spend some quality time with the family, the US waters have a lot to offer. You can enhance your experience by visiting some of the top locations. Professional services like Dupe A Fish can help you in planning a great trip at affordable prices. Keep in mind the following places for a fun and memorable fishing trip in the USA.

Florida

Florida is known as the "Fishing Capital of the World" due to its great commercial fisheries, subtropical climate, scenic locations, infrastructure, and management. Being a peninsula, Florida is surrounded by water on both sides, offering several spots for fishing. You can find an abundance of tarpon, sailfish, and cobia, among the other fish. You can explore the fishing spots better by hiring one of the best charter boats for fishing in Florida. Another reason to go fishing here is the exceptional surroundings. The scenic locations of the Floridian lakes and rivers add more life and vibrance to the fishing experience.

Alaska

Alaska is another popular destination in the US to go fishing. It is a large state with fresh waters and exquisite natural wonders. Not only does this place offer anglers a tournament-grade experience, but a wholesome trip if you are planning to bring your friends and family along. Anglers prefer to visit Alaska for many reasons. You can find a diverse variety of fish in the Alaskan waters, such as blackfish, hagfish, halibut, lingcod, mackerel, salmon, trout, and more. At every turn, you will come across pristine waters and a scenic view to take your trip to the next level. To make the experience better, people often hire fishing trip charters in Alaska.

California

With its diverse and quality fishing spots, California offers anglers an exceptional fishing experience. A variety of fish species, like a pacific lamprey, white sturgeon, chinook or king salmon, rainbow trout, pink salmon, bass, striped bass, and halibut, are present in the Californian waters. There are many spots to go for fly fishing trips in California with friends, family, or solo.

Are you considering going on a fishing trip? Dupe A Fish offers its remarkable assistance in helping you plan the best trip as per your needs.

Author: Christian Bacasa

Dupe a Fish, LLC.

Find a trip that is perfect for you or your group. We are working to have the widest variety of guides and services available on the web and specialize in offering trips that are perfect for you, your group, and your budget. Start defining your perfect trip now. We make it easy!

Connect with us on Social Media

Instagram Facebook Twitter YouTube LinkedIn Pinterest

Want to learn more about fly fishing? Subscribe to the Fly Fishing Insider Podcast

[articlepublication]

Choosing the Right Company for Video Production

Video marketing offers high value to many businesses today. Whether you want to convey your life story, explain concepts, or market a product or service, filming a video for such a purpose often allows you to successfully reach out to and engage with your target audience. Having an experienced video production company to help you with your marketing strategy is a great idea. Truerity Digital is well-known for its video production services in Asheville, NC. Finding and choosing the right company may consume time and effort, but it is always worth the wait. To make the job simpler, consider the following tips for informed decision-making.

Check the portfolio

One of the most important things to consider is the company's portfolio, which showcases its abilities and videography style. Going through their portfolio gives you a better insight into making a decision based on their work quality and whether they can meet your requirements. Before choosing a company, make sure to ask for their portfolio for reference.

Experience matters

You must have noticed that whether it is a company or an organization, people prefer hiring people with experience over those with none. Similarly, when you are on the lookout for a video production company, it is better to choose one with relevant experience. You may want to give a novice an opportunity, but if you are not willing to experiment, it is best to go for experienced professionals. Truerity Digital is a well-known business with over 30 years of experience in digital conversions in Asheville, NC.

Quality of work

The aim of availing such services is to produce a high-quality video. And one can achieve that with the help of reliable experts who offer excellent solutions. It is important to look for a production company that can provide you with a certain production value standard.

Budget-friendly

Another thing to keep in mind is the amount that you are willing to spend on these services. Make sure to create an estimated budget in advance to help filter and find suitable companies easily.

Timeframe

Time efficiency is another crucial factor to consider. Specify your deadline to the professionals so that they can manage the video production on time. Also, check if the company has a good history of meeting deadlines.

Truerity Digital is a well-known company with an experience of more than 30 years in a variety of exceptional services such as video production and digital conversions in Asheville, NC. They use the latest technology to provide high-quality solutions to match their clients' vision at affordable prices. For more details, call (704)641-7867.

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The Researcher’s Guide to Magnetic Beads

Magnetic beads are widely used for a number of applications in molecular biology, such as NGS(next-generation sequencing), PCR (polymerase chain reaction), qPCR (quantitative PCR), other genotyping applications, and protein purification. This guide will help you ensure accurate, reproducible resultswhile working with magnetic beads.

Magnetic Beads

Magnetic beads aretiny particles (20 to 30 nm) of iron oxides, which give them superparamagnetic properties. These magnetic beads are unique because they exhibit magnetic behavior only in the presence of a magnetic field.

Thebeads’ paramagnetic propertyallows them to be separated in suspension, along with anything they are bound to. Since they don’t attract each other without an external magnetic field, they can be safely used without any unwanted clumping.

This makes magnetic beads, or mag beads, easy to use,automation friendly and ideal for a wide range of applications.

Magnetic Separation

Magnetic beads are used in molecular biology applications as a reliable method of purifying various types of biomolecules.

With an appropriate surface coating and under optimized conditions, the target molecule can selectively bind to the beads, leaving any impurities in the solution. The purified target moleculecan then be used directly in molecular biology applications.

One of the most important advantagesof using magnetic beads is they allow different types of biomolecules to be isolated directly from a variety of different crude sample types with minimal processing. Such versatility and simplicity make magnetic beads superior to any other methods of nucleic acids’ and other biomolecules’ isolation.

Magnetic bead DNA extraction

First, the DNA is allowed to bind to the magnetic beads. Then, an external magnetic field is applied to attractthe beads to the outer edge of the containing tube, and immobilize them. During washing and contaminant removal, the beads and the bound DNA remain immobilized. Elution buffer is added to separate the DNA from the immobilized beads.The purified sample is ready for quantitation and analysis. Lastly, the magnetic field can be removed to release the beads for re-use. No centrifugation or vacuum is needed.

Magnetic bead DNA extraction:

requires less steps and reagents compared to other DNA extraction methods

minimizes stress or shearing forces on the target biomolecules

can be automated in 24, 96, and 384-well plates

Applications of magnetic beads

Different surface coatings allow each type of bead different binding properties. As a result, magnetic beads can be used in the following applications:

Nucleic acid – DNA, RNaA, plasmids and mitochondrial DNA – isolation and purification

Protein isolation and purification

Size selection

Separation of specific cell, organelle or target types

Magnetic activated cell sorting (MACS)

If you are looking for high-quality magnetic bead-based kits for nucleic acid isolation and sample collection and stabilization tubes, call one of the most trusted RNA extraction kit manufacturers in USA, MagBio Genomics at (301) 302-0144.

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How does a video production company help you?

One of the most creative and dynamic media is video. Whatever the purpose, you can create a video for it. But in the professional world, it has a totally different meaning. It is much more complex than making a random video using your phone's camera. It involves a lot of work, specialized equipment, and time. Did you know that video production plays a significant role in the marketing industry? After all, a perfectly shot video is engaging and helps rope in potential audiences towards businesses. But if you are new to all this, you might wonder, what does a video production company do? We have covered all that you need to know in this article.

Typically, there are three principal things that video production companies do: pre-production, production, and post-production.

Meeting clients

The first step of a pre-production stage is to meet up with potential clients. In this meeting, the client and service providers discuss the crucial details, such as the idea, budget, deadline, content matter, target audience, and the objective of the video. A detailed meeting helps both parties to be on the same page.

Research and analysis

After understanding the client's requirements, the professionals thoroughly research them. Proper analysis helps understand the work of clients and their audiences. Additionally, it also helps the experts in gathering great ideas to drive the growth of the client's business and make them stand out from the competitors.

Storyboarding

The next step after researching and setting the goal is storyboarding. It involves planning the video shots through graphic representations. Although the result can be different from the storyboard, this step is necessary to keep the filming stage on track.

Making scripts

Although storyboarding offers a graphical representation of the shots, you also need some dialogues and voiceovers in the video. Writing scripts helps plan out when and what is to be said and the delivery tone to meet the client's goal.

Talent search and props

The video needs a face for it. It cannot be just any face, but a person who delivers the content and/or acts. An actor with skills is needed. And in addition to that, video production also requires props to use in the video.

Filming location

It is the last step of the pre-production. Most often, videos are shot in the studio itself. Also, as per the client's request, experts may look for some other locations. But it is important to consider the following factors:

● The travel distance

● Any possible sources that may create background noise

● Permit for shooting video

● Insurance coverage necessities

Production

Shooting a video may take a single day or a few days, depending on the client's need. To film a video, you require various tools and equipment. Most companies have high-resolution cameras, lighting, audio equipment, etc. Moreover, it can take some extra time due to reshoots.

Editing

The pre-production phase involves doing the final touch-up to the filmed shots. Video production and editing mean trimming and combining different footage. Editors also add special effects like animations, subtitles, sound effects, and other visuals.

Sound Mixing

The final step for producing a professional video is to add sound to the film. The recorded audio is edited and added accordingly. And after finishing the video, the production house provides the final result to the client.

Are you looking for video production and editing services in Asheville, NC? TruErityDigitalhas experience for over 30 years in providing quality and trusted services for video production, digital conversions, and more in Asheville, NC. To learn more, call at (704)641-7867

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The Simple Guide to Fishing in California

From an angler's viewpoint, California is no less than a paradise. California is the third-largest state in the US, with beautiful coastlines running from north to south, offering vast possibilities for fishing. Each year, a large number of fishing enthusiasts visit with families and friends, and even solo fishing boat trips in California.

California fishing license and permit

The public pier is a free spot to fish, and anglers do not require any license. However, you can have a better experience and find a diverse range of fish in the Californian rivers and lakes. The California Department of Fish and Wildlife (CDFW) provides the sport fishing license. You can apply for one online, and they will mail all your documents within 15 days. There are a number of fishing licenses available. These licenses are valid for a year, one, two, or ten days, as per your requirements. You might notice a difference in the prices by your state of residence. If you are lucky, you may also get to go fishing in California for free as the CDFW hosts two annual cost-free fishing days.

The best fishing spots in California

After getting a license, you are all set to go fishing in California. There are numerous locations to suit your fishing needs, from lake fishing to deep-sea fishing. But if it is your first time there, it is best to hire professional fishing guides in California.

Big Bear Lake

Fishing is a popular summertime activity in Big Bear Lake. Here, anglers can find different kinds of fish, such as rainbow trout, largemouth bass, channel catfish, and panfish. You can find the best spots along North Shore Drive between Windy Point and the Bear Valley Dam.

Lake Isabella

To catch trout, bass, bluegill, crappie, and catfish, head to Lake Isabella. You can find it in the southern part of the Sequoia National Forest. If you plan on fishing from a boat, a permit is mandatory. Not only is this place great for fishing, but you also get to enjoy a scenic view while you are at it.

San Diego

San Diego is a perfect choice for anglers who prefer deep fishing. If you are here in the winter seasons, you get to catch rockfish, lingcod, sculpin, and sheepshead whereas, during the summer months, you will find yellowtail, barracuda, and bass.

San Joaquin Delta

San Joaquin Delta is a fishing spot that you may find on almost every angler's bucket list. All year long, you can fish bluegill, sturgeon, striped bass, catfish, salmon, and American shad in these waters.

Dupe A Fish can help you book your fishing boat trips in California. For more details, visit their official website here.

Author: Christian Bacasa

Dupe a Fish, LLC.

Find a trip that is perfect for you or your group. We are working to have the widest variety of guides and services available on the web and specialize in offering trips that are perfect for you, your group, and your budget. Start defining your perfect trip now. We make it easy!

Connect with us on Social Media

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[articlepublication]

Effectiveness of Natural Supplements for Tourette Syndrome

Many children between the ages of 2 and 15 years experience a condition of the nervous system, Tourette syndrome. It causes the sufferer to experience tics, which are repetitive involuntary movements, twitches, and sounds. So far, TS is incurable, but there are a few ways to lessen the impact of this disease.

Complications

Over the years, tics can become less daunting. With age, the patients learn to deal with it and control its impact. However, in the early years, these can cause a lot of difficulties. TS can take a toll on the sufferer's behavior and lower their self-esteem. Some of the common complications that people face are:

● Sleep disorders

● Conflicts and anger management issues

● Depression

● Anxiety issues

● Attention-deficit/hyperactivity disorder (ADHD)

● Learning disabilities

● Pain due to tics

● Autism

● Obsessive-compulsive disorder (OCD)

Another beneficial way to cope with TS is natural supplements for Tourette Syndrome. High-quality natural supplements contain herbal ingredients that have been used for a long time by ancient medicine practitioners to successfully help with various health conditions. For example, the 5-Ling formula of the TS Support supplement by Naturekue is a proprietary polyherbal formulation of Paeoniae Radix Alba (Baishao), Gastrodiae Rhizoma (Tianma), Ganoderma (Lingzhi), Scutellariae (Huangqin), Tribuli Fructus (Jili), Uncariae Ramulus Cum Uncis (Gouteng), Polygoni Multiflori Caulis (Shouwuteng), Ziziphi Spinosae Semen (Suanzaoren), Schisandrae Chinensis Fructus (Vinegar Steamed, Wuweizi), Arisaema Cum Bile (Dannanxing), Gardeniae Fructus (Zhizi). The TS Support 5-Ling formula is clinically proven and supported by data to help children suffering from Tourette syndrome in a number of ways. It improves brain function, focus, and concentration as well as helps maintain normal function of the body for daily activity. In addition, TS Support relieves stress, frustration, and fatigue.

Enhanced concentration levels

A person suffering from TS does not have any direct control over the tics. These can happen to them at any time and any place, which can cause the person to lose their focus. The natural Tourette Syndrome supplements are blended with ancient herbs that help in keeping the mind alert and improve focus. This enables the child to cope with life at school too.

Stress management

Whether the tics involve body twitches or sounds, they can take a massive toll on the person’s quality of life. Naturekue's TS Support is a clinically proven supplement that contains natural ingredients, such as Polygoni Multiflori Caulis, which has an exceptional calming effect on the nerves and eases the mind.

Since natural herbal supplements contain natural, herbal ingredients with rich healing and therapeutic qualities, they offer effective relief and boost the functioning of the brain.

Naturekue offers TS Support - the proven Tourette Syndrome supplement in the USA. These herbs help kids get back to their regular lives by controlling unwanted outbursts, fighting verbal tics, and managing facial spasms.

Disclaimer: This article is not meant to treat, diagnose, or prevent any disease; it is for informational purposes only, so make sure to consult with your physician and pharmacist before beginning with any new meds, nutritional diet, or supplements.

[articlepublication]

Significance of DNA Library Normalization for NGS and Options Available

Normalization in NGS or Next-Generation Sequencing refers to equalizing the concentration of DNA libraries for multiplexing.

Multiplexing enables you to run multiple libraries on a single flow cell and reduces costs by maximizing the use of the ever-increasing capacity of NGS.

The lower DNA sequencing costs have made it possible to use in a wide range of molecular diagnostics applications, including reproductive health and oncology, as well as clinical research.

Uneven library concentrations from different qualities and quantities of samples can lead to inconsistent data. Libraries with a high concentration may be overrepresented on the flow cell and vice-versa. Hence, normalization is essential to ensure every library is equally represented and sequenced to sufficient depth.

Without normalization, you may have to spend additional time re-preparing libraries, if the sample is available. You would rather use this time on downstream analysis or preparing the next batch of libraries.

In addition, if your analysis is based on incomplete or inaccurate data, it will lead to missing out on key information, unreliable results or you would have to repeat experiments.

Comparing DNA quantitation and normalization methods

There are various options for quantitating library preps that vary inaccuracy.

a. Spectrophotometry - detects the absorption of UV light by molecules in the sample.

Advantages: Quick, low cost

Disadvantages: Inaccurate, measuring all nucleic acids, not just adaptor-ligated molecules; not very sensitive; affected by contaminating RNA and proteins

b. Electrophoresis - estimates fragment sizes through capillary electrophoresis and concentration through intercalating dyes.

Advantages: Accuracy in fragment size and distribution estimation

Disadvantages: Quantitation cannot differentiate between adaptor-ligated and other molecules; expensive equipment requirements for a single purpose

c. Fluorometry - uses fluorescent dyes to assess the concentration of nucleic acids against a standard curve.

Advantages: Sensitive and accurate estimation of dsDNA concentration; can be used to specifically quantitate single-stranded DNA, RNA, and protein; fast; low cost

Disadvantages: Cannot differentiate between adaptor-ligated and other molecules; not able to estimate fragment size

d. Quantitative PCR (qPCR) - adaptor-specific primers are used with fluorescent dyes and quenchers to quantitate library preps against standard curves.

Advantages: Accurate quantitation of adaptor-ligated molecules (viable sequencing templates); High sensitivity (suitable for quantitation of dilute libraries); Amenable to automation

Disadvantages: High cost; requires more hands-on time

While no single method provides all the data and accuracy you need for normalization, qPCR seems to offer the most accuracy in quantitation.

Magnetic beads-based normalization

Imagine that you didn’t need to go through quantitating your libraries at all!

Magnetic beads are already being used as a reliable and established way to safely handle nucleic acids.

Magnetic bead-based normalization is based on the principle that a given volume of beads can bind a consistent quantity of nucleic acid molecules. All unbound molecules are then washed away and each library is represented by just the bead-bound molecules.

Different coating options are available to suit various applications. For example,

a. carboxyl- and silica-coated magnetic beads can be used for generic, non-specific binding based on buffer conditions

b. oligo(dT)-coated beads for binding mRNA

c. streptavidin-coated beads for binding biotinylated samples

Studies indicate that bead-based normalization allows more consistent read depth than other quantitation-based methods.

Are you looking for effective and reliable magnetic bead-based kits for the normalization of DNA concentration? Call MagBio Genomics at (301) 302-0144.

[articlepublication]

All You Need To Know About 8mm Movie Conversion

There was a time when VHS and 8mm films were the technological breakthrough. Back then, most people used them to record important life events. However, they are now obsolete. Nevertheless, you may have many precious memories on those tapes. Those recordings may be the only connection you have left with the good old days and long gone precious moments. Fortunately, you now have digital conversion technology available to convert these tapes and films into modern formats and save them for a long time.

How do you benefit from 8mm movie conversion?

Prevent the loss of data

8mm films were all the rage several decades ago. These films are fragile and vulnerable to damage easily. Once damaged, you won’t be able to retrieve any data from them. The good news is you can preserve and safe-keep the data with 8mm movie conversion. Modern formats allow you to store your data safely for a long time to come.

Preserve memories

8mm movie was used by many people in the past for recording videos and creating memories. As the technology evolved, they went out of the market, and now these are very rare to find. In addition to that, these can deteriorate easily with time. Converting these films into a digital format through digital conversions helps you preserve these memories for future generations.

Share easily

Going through these old memories and photos can make anyone nostalgic. You may want to share them with your loved ones. After digital conversion, videos can be easily shared with anyone, without any difficulty. You can also have copies made, which means everyone in your family can relive and enjoy those moments whenever they want to.

Convenience

Even if your old 8mm film is intact and in pristine condition, you may not have the equipment to watch them. Having those films and not being able to watch them is worse than not having them. By digitally converting those films, you can get more convenient access to the old films.

Clears space

The 8mm films and tapes can take up space at home. Converting these movies into digital format can help clear that space. You can store these digital conversions on your phone, PC, laptops, external hard drives, even the cloud. In addition to clearing considerable physical space, digital formats also protect the videos and memories from getting lost or damaged physically.

TruErity Digital is a privately-owned business that specializes in the digital conversion and preservation of treasured memories from your life’s journey with over 30 years of experience. With the help of advanced technical tools, they offer reliable services for reel-to-reel movie conversion, 8mm movie conversion, and digital conversions in Asheville, NC. If you are looking for digital conversion services, call the professionals at (704)641-7867

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6 Must-Visit Fishing Boat Trip Locations in California

California is popular among anglers who take fishing boat trips! Fishing is a unique sport that you can enjoy solo or in a group. California has multiple locations for fishing boat trips. All you have to do is know the basic rules and regulations of fishing as stated by the California Department of Fish and Wildlife beforehand, pack your gear, hire a charter for fishing in California and enjoy!

The San Joaquin Delta

If you are an avid angler, you either have the San Joaquin Delta on your bucket list or, you have already been there on your California fishing boat trip. With water running for hundreds of miles, you can have a great time here. You are most likely to catch catfish, bass, and sturgeons here.

Clear Lake

In Northern California, Clear Lake is a famous spot to go fishing and catch largemouth bass, bluegill, catfish, and more. There is a hiking trail nearby, meaning you can enjoy hiking too!

Lake Cuyamaca

You can visit Lake Cuyamaca at any time during the year and will come across plenty of rainbow trout and bass fish. Every year, anglers from all around come to this spot to spend their day fishing.

Lake Shasta

If you are looking for a spot in California to catch different kinds of fish, there is no place better than Lake Shasta. You get to enjoy fishing all year round with the lake stocked with a variety of fish. However, before you set sail in the waters, make sure to get a license. Then all you need is your fishing gear, a charter, and fishing guides for the best experience in California!

Santa Monica Pier

In the Southern California region, you will come across a famous tourist spot, which also happens to be an incredible place for fishing! The Santa Monica pier attracts many anglers to catch and fish calico bass, rainbow perch, black cod, sargo, mackerel, and a lot more!

Coronado Ferry Landing Pier

Although this fishing spot is not known to many, if you are in California and want to catch some saltwater fish, the Coronado Ferry Landing Pier is the place to go. Bay bass, Corvina, mackerel, halibut, and bonito are found in abundance here.

Fishing boat trips in California are always fun. If you are planning on going on one, you can find the best help at Dupe A Fish. They can help meet all your requirements and ensure that you have a great trip ahead!

Author: Christian Bacasa

Dupe a Fish, LLC.

Find a trip that is perfect for you or your group. We are working to have the widest variety of guides and services available on the web and specialize in offering trips that are perfect for you, your group, and your budget. Start defining your perfect trip now. We make it easy!

Connect with us on Social Media

Instagram Facebook Twitter YouTube LinkedIn Pinterest

Want to learn more about fly fishing? Subscribe to the Fly Fishing Insider Podcast

[articlepublication]

5 Effective tips for PCR primer design

Primers are at the core of the PCR (polymerase chain reaction). Understanding the implications of primer design will help you plan successful experiments. If you are interested in PCR optimization and validation, here are some tips that will help you avoid common PCR pitfalls.

PCR primers and assay performance

Theoretically, efficient PCR should double the target amplicon at each cycle. But if you have been doing this for some time, you know there are several challenges – low yield, unexpected PCR artifactsor the wrong amplicon.

PCR primers are interdependent with the type of the sample, reagents, targetand thermodynamic conditions. Having a clearly defined PCR objective can help you make the right primer design and enzyme selections.

Primer melting point

The melting temps (Tms) for primer pairs should bewithin 5°C of each otheras that affects the annealing temperature of the reaction. The melting temperatures should range from 50-72°C to avoid weak thermodynamics because that can cause formation of primer dimers due to inefficient binding. You can increase specificity with Touchdown PCR.

Primer concentration and quality

Start with the recommended final concentration for the specific polymerase PCR protocol, and optimize from there, if needed. Measure the concentration with a spectrophotometer. Higher primer concentrations lead to an increased risk of secondary priming and false amplification products. On the other hand, low concentration will impact assay linearity visible with qPCR. Desalt or HPLC purify the primer formulations to avoid oligo manufacturing by-products that reduce PCR efficiency.

Primer length

Primers should be 20-30 nucleotides long. The specificity can be refined with primer length. Heterogeneous sample types need relatively long primers for higher primer specificity. The primer should not be able to recognize multiple binding sites in a genome, else they’ll producemany off-target products. Artifactual recombinant PCR products may be formed if partially extended primers anneal to partially homologous target sequences.

Primer structure

Complementarity may allow two primers to hybridize at their 3´ ends. This may result in a polymerase extending to form dimerized PCR products. If your reaction primers get tangled with hairpin loop secondary structures within individual primers, they can slow down DNA polymerases.

Primer storage

Primer degradationcould be the culprit if your PCR assay stopped working. You should avoidprimer degradation that is caused due to multiple freeze-thaw cycles during storage.

If you are looking for viral DNA/RNA extraction kit and RNA extraction kit manufacturers in USA, call MAGBIO Genomics at 301-302-0144. MAGBIO Genomics is the industry leader in magnetic bead-based products for nucleic acid isolation and DNA, cfDNA, and RNA extraction.