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blogpublication · 8 months ago

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High Molecular Weight DNA Isolation: Why MagBio's Magnetic Beads are the Ultimate Ampure XP Alternative

High molecular weight (HMW) DNA isolation is a critical process in modern genomics research, especially for applications like next-generation sequencing (NGS), long-read sequencing, and genome assembly. Scientists rely on high-quality, intact DNA to ensure accurate results and reproducibility. In the quest for precision, researchers often turn to magnetic bead-based reagents for PCR clean-up and size selection to achieve superior results.

While the Ampure XP system has long been considered the gold standard for DNA purification and clean-up, MagBio’s HighPrep PCR magnetic beads have emerged as a powerful and efficient Ampure XP alternative. Designed specifically for PCR clean-up and size selection, HighPrep PCR offers significant advantages, especially for High Molecular Weight DNA Isolation, making it the go-to solution for many researchers looking to optimize their workflows.

High Molecular Weight DNA Matters

Before diving into the benefits of MagBio’s HighPrep PCR, it's important to understand why high molecular weight DNA isolation is so essential. HMW DNA refers to DNA fragments that are longer and more intact than standard DNA fragments. These long fragments are especially important in applications such as long-read sequencing, structural variant analysis, and genome assembly, where large DNA molecules provide a clearer picture of genetic architecture.

Accurate High Molecular Weight DNA Isolation ensures that DNA is not fragmented or degraded during extraction and purification. Longer DNA fragments lead to better sequencing results, more comprehensive genome coverage, and a deeper understanding of genetic complexity. To achieve these results, researchers require reliable purification systems that preserve DNA integrity and enable efficient downstream analysis.

Drawbacks of Ampure XP

While Ampure XP has served as the gold standard for PCR clean-up and DNA purification, it is not without its limitations. One of the major drawbacks is that the Ampure XP system can sometimes lead to loss of HMW DNA fragments during size selection, especially for applications where precise selection of long DNA fragments is necessary. Additionally, Ampure XP requires precise buffer-to-sample ratios to achieve optimal performance, which can complicate workflows and introduce variability.

In an era where speed and efficiency are crucial, many labs are also looking for more automation-friendly solutions that integrate smoothly with liquid handling systems. Ampure XP’s protocol can be cumbersome for high-throughput applications, making it less ideal for labs that require quick turnaround times and scalable workflows.

HighPrep PCR: The Superior Ampure XP Alternative

MagBio’s HighPrep PCR offers a powerful solution to the limitations of Ampure XP, providing superior PCR clean-up and size selection for both standard and high molecular weight DNA. This paramagnetic bead-based reagent is specifically designed for efficient DNA purification, ensuring high recovery rates and consistent fragment size selection, making it ideal for applications like NGS, cloning, and PCR clean-up.

High Recovery of Amplicons >100 bp

One of the key benefits of HighPrep PCR is its ability to achieve high recovery rates of DNA fragments, especially those greater than 100 base pairs. This feature makes it particularly useful for isolating longer DNA fragments, which is essential for applications such as long-read sequencing and HMW DNA isolation. Unlike other reagents, which may lose significant amounts of long DNA fragments, HighPrep PCR ensures maximum recovery, preserving the integrity of your DNA samples.

Efficient Size Selection for High Molecular Weight DNA

Size selection is a critical step in DNA library preparation for sequencing. HighPrep PCR’s magnetic bead-based system allows for precise and uniform size selection, ensuring that only the desired DNA fragments are retained for downstream applications. This is especially important for High Molecular Weight DNA Isolation, where preserving longer DNA fragments is key to successful sequencing.

HighPrep PCR selectively binds to DNA fragments based on the concentration of beads used in the reaction. This flexibility allows researchers to fine-tune their size selection to meet specific experimental needs, whether they are working with fragmented DNA, amplicons, or genomic DNA.

No Centrifugation Required

Traditional DNA purification methods often require time-consuming centrifugation steps, which can be prone to error and introduce variability into workflows. HighPrep PCR eliminates the need for centrifugation by using magnetic beads to bind and isolate DNA fragments. This not only streamlines the process but also reduces the risk of sample loss or contamination. For labs looking to increase throughput and efficiency, this is a major advantage.

Automation-Friendly Design

As more labs move toward high-throughput automation, having a PCR clean-up reagent that integrates seamlessly with liquid handling systems is essential. HighPrep PCR is designed with this in mind, offering compatibility with most liquid handling stations. This makes it an excellent choice for labs that need to scale up their operations without sacrificing precision or reproducibility.

Automation-friendly reagents like HighPrep PCR help reduce human error, save time, and allow for more consistent results. In contrast, Ampure XP may not always offer the same level of integration with liquid handling systems, making it less suited for high-throughput applications.

Consistent Fragment Size Distribution

Achieving uniform and consistent DNA fragment sizes is essential for reliable results, especially in applications like NGS. HighPrep PCR ensures that DNA fragments are consistently sized, leading to better library quality and more accurate sequencing data. This is particularly beneficial for researchers working with complex or degraded samples, where maintaining consistency across replicates is crucial.

Flexible DNA Input Range

HighPrep PCR is highly versatile and can accommodate a wide range of DNA input concentrations, from 0.5 ng/µL to 150 ng/µL. This flexibility makes it suitable for various sample types, including HMW DNA, fragmented DNA, PCR amplicons, and genomic DNA. Whether you’re working with low-concentration samples or need to scale up for large projects, HighPrep PCR offers the adaptability needed to ensure successful DNA purification and size selection.

Rapid and Reliable Results

In a fast-paced research environment, speed is critical. HighPrep PCR offers a rapid protocol that can be completed in under 65 minutes, allowing researchers to move quickly from PCR clean-up to downstream applications. This saves valuable time without compromising on the quality or reliability of the results.

Applications of HighPrep PCR

MagBio’s HighPrep PCR is designed to meet the needs of researchers across a wide range of applications, including:

NGS Library Preparation: HighPrep PCR ensures that DNA libraries are free from contaminants and have consistent fragment sizes, leading to higher-quality sequencing data.

PCR Clean-Up: Remove unwanted primers, dNTPs, and salts after PCR to obtain pure DNA for downstream analysis.

DNA Size Selection: Achieve precise and uniform size selection for long-read sequencing or other applications requiring HMW DNA.

Cloning: Ensure that only the desired DNA fragments are retained for cloning applications.

Microarrays and Restriction Enzyme Digestions: Purify and size-select DNA for use in microarray and restriction digestion workflows.

When it comes to PCR clean-up and size selection for High Molecular Weight DNA Isolation, MagBio’s HighPrep PCR stands out as the ultimate Ampure XP alternative. Its ability to recover long DNA fragments, perform efficient size selection, and integrate with high-throughput automation makes it the preferred choice for labs looking to optimize their DNA purification workflows. By switching to HighPrep PCR, researchers can achieve better results, faster processing times, and more consistent data—all while preserving the integrity of their valuable DNA samples. If you are looking for Magnetic beads for PCR Clean Up and Size Selection, High Molecular Weight DNA Isolation, or Ampure XP alternative, check out MagBio Genomics, Inc..

#healthcare #DNA/RNA extraction kit #PCR Clean up #DNA Normalization kit

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foodandbeverages · 10 months ago

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Food Authenticity Testing Services Market Earnings Margins, Value Of Production & Consumption Demand Figures 2023 to 2033

In 2023, the US$ 5893 million for the food authenticity testing service market (Markt für Dienstleistungen zur Prüfung der Lebensmittelauthentizität) is estimated to reach a revised size of US$ 10112.8 million by 2033. This market is expected to expand at a CAGR of 6% during the analysis period.

Consumers have become increasingly skeptical about the authenticity of food products designed to meet specific needs, such as vegan, free-form, and organic. This is due to intentional ingredient substitution, misleading consumers with false claims, and disruptive labeling.

To differentiate their products from conventional offerings, food manufacturers have adopted food authentication techniques to capture significant market share. Consumers are increasingly concerned about food safety and clean labels, so adoption may be on the rise.

The increasing incidence of fraud, false labeling, certification, and adulteration in food products is one factor driving the market growth. Additionally, consumers are becoming more knowledgeable about food quality, increasing market growth.

Detailed Market Study: https://www.futuremarketinsights.com/reports/food-authenticity-market

“The epidemic of economically motivated infestation (EMA) to overcome competition has forced governments to impose strict regulations on food inspection and authenticity regulations, pushing markets toward a multi-billion-dollar valuation.” – says a lead analyst at Future Market Insights.

Key Takeaways from Market Study

The food authenticity testing service market is expected to grow at a CAGR of 6% over the forecast period.

It is estimated that the food authenticity testing service market in North America will remain strong during the forecast period.

Due to its large population, Asia Pacific is expected to be the fastest-growing region for food authenticity testing services

Testing meat speciation authenticity is bolstered by a high incidence of meat consumption

The food authenticity testing services market in China is expected to witness significant growth at a CAGR of around 4.4%.

Competitive Landscape:

The global food authenticity testing service market is dominated by a small number of players aiming to concentrate their presence in emerging markets. They launch products, introduce innovations, acquire strategic companies, and establish laboratories and research facilities in areas that are not developed. By offering innovative products, market players can maintain supply and demand, thus assisting the growth of the overall market.

Several prominent companies dominate this market, including ALS Ltd, EMSL Analytical, Inc., Genetic ID NA Inc., Eurofins Scientific SE, Merieux NutriSciences Corporation, Intertek Group PLC, Microbac Laboratories Inc., SGS SA, Romer Labs, others.

Recent Developments:

In collaboration with Eurofins GeneScan Technologies GmbH in October 2019, UgenTec’s real-time PCR analysis software platform, FastFinder, may be used to develop assay plugins for Eurofins GeneScan Technologies’ portfolio of molecular biology kits. Furthermore, the partnership can help Eurofins scale up its assays with automated results reports and fast sample-to-result times for food, feed, and seed testing.

With the acquisition of Vanguard Sciences Inc. in January 2018, SGS expanded its global network of agriculture and food laboratories by integrating the company’s two food laboratories. Agricultural and food testing is provided by two laboratories, including product testing and research (including testing of processes and products).

SGS announces the opening of its new food testing laboratory in Papua New Guinea. In addition to food safety, quality, and sustainability, SGS offers a comprehensive range of services.

Food Authenticity Testing Services Market Segmentation by Category

By Food Tested:

Meat & Meat Products

Dairy & Dairy Products

Processed Foods

Other Food Tested

By Target Testing:

Meat Speciation

Country of Origin & Ageing

Adulteration

False Labelling

By Technology:

PCR-Based

Liquid Chromatography-Mass Spectrometry (LC-MS)

Isotope

Immunoassay Based/ELISA

Other Technologies

By Region:

North America

Latin America

Europe

The Middle East and Africa

East Asia

#Food Authenticity Testing Services

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thomasjanson · 4 years ago

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Compared to PCR fragments cloning, direct sequencing of PCR products is highly convenient when it comes to sequence analysis. During the preparation of direct sequencing, it is essential to verify the desired product and confirm the lack of primer dimers. Continue Reading>>

#PCR Clean-Up Kit

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liu-lang · 3 years ago

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i finished my 10 day quarantine on tuesday, dec 28.

on wednesday, dec 29, i tried to join a virtual waitlist for testing but by the time i texted at 08h on the dot, it said it was at capacity. i rang the urgent care the day before and they told me that there was a 15 min window 08h to 08h15 where people could join the 80 person capacity waitlist via text. no idea what happened when i tried to join. i was bummed out.

luckily though i ordered a labcorp on demand at home test kit. if you go on their website now, they have a little notice saying that they're not accepting any new orders from from dec 29 to jan 2. so i feel really lucky that i had the foresight to order one right when i tested positive. i took my sample and scheduled a fedex pick up. the delivery person who fetched it from the lobby held it as far away from him as possible even though he didn't have anything to worry about since it's sealed in a biohazard bag.

then i went outside for the first time in 10 days - everything felt the same as the last time i had been outside - cold, wet and rainy with long queues of people everywhere. i wanted to find a rapid antigen and PCR test. first i went to the pharmacy that gave me the false positive the first time - i figured i would just try there in case i was unable to queue anywhere else. this must be a secret testing site bc there were only 2 ppl ahead of me. i got my first negative from a rapid antigen test there.

then i went to find a mobile test van. while i was signing up, i saw one of the people i queued with 10 days ago at urgent care - she wore the same yellow coat - we recognised each other. she turned out to be negative last time and i shared how i was positive and my experience getting better. it was nice to see a familiar face.

unfortunately the mobile test van got hit by a car. it was jarring but they continued testing. i got my second negative rapid antigen test then. at this point, i emailed my manager at my 2nd federal work study job my results and she said i could come back to work on monday, jan 3 since they closed the office for cleaning bc more of my colleagues have covid.

i'm really grateful that i got better. it was interesting that even though i was boosted, i had way worse symptoms than my roommate who didn't have her booster. i was most concerned in beginning and then in the middle when i started to have worse chest pain and was coughing up blood. my roommate's symptoms improved everyday while mine got worse and then thankfully plateaued.

right now i have a lingering cough and a lot of phlegm so i have an appt with my PCP next week to check my lungs.

#personal #nyc move #covid 19

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cedar-glade · 5 years ago

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Extended cell access and targetting summerized in light reading detail, and current issues with fast test methods(Expansive reasons for false negatives/positives aside from asymptomatic exposures) This does not include a summery on Spike light and heavy chain mutation and RNA replication transposon insertion mutation. This also does not include the current treatment protocal and the current concepts inline with UMAP analysis and Proteomic prompts.

Covid- 19 review and Associated Fast Treatment Methods Review

John Potter/ 05/ 07/ 2020

COVID-19(SARS COV 2), a member of the corona virus family, Coronaviridae, and a linked relative to the original SARS- COV, is an airborne pathenogenic retro virus; “Coronaviruses (CoVs), enveloped positive-sense RNA viruses, are characterized by club-like spikes that project from their surface, an unusually large RNA genome” (Fehr, Perlman et. al. 2015)(WHO, 2020). While airborne transmission is the open cause and primary cause noted it may not explain the mechanism in full; “Human-to-human transmission is primarily achieved through close contact of respiratory droplets, direct contact with the infected individuals, or by contact with contaminated objects and surfaces”(Malik Ya, 2020). COVID-19 is named this due to it’s Chinese origin and the year it was isolated but it’s legitimized name in virology is SARS COV 2(WHO, 2020). It functions as a primary pathogenetic virus that effects immune response and becomes more damaging in the presence of a secondary pathogen (such as bacteria that cause pneumonia), or in immunocompromised subjects (Zheng et. al. 2020) (Leng et. al. 2020). It functions by causing irritation, over production of mucus, and inflammation in direct correlation to infection of specific cell tissues in the upper lower respiratory tract, “mainly invades alveolar epithelial cells” (Zheng et. al. 2020). The mode of infection mechanisms works by targeting specific cells in the respiratory tract that are ACE2 heavy, progenitor cells that may be peripheral primordia or end up elongating into cilia cells that clean debris from lungs(WHO, 2020)(Zheng et. al. 2020). Other specified areas of respiratory periphery cells responsible to upkeep that can also have serious detrimental effects after infection are found in the bronchii; “bronchial transient secretory cells” (Lukassen et. al. 2020). “ACE2 is the main host cell receptor of 2019 CoV and plays a crucial role in the entry of virus into the cell to cause the final infection” ( Xu. et. al. 2020). “Angiotensin-converting enzyme 2 (ACE2) is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems” (Zheng et. al. 2020). COVID-19 opperates specifically systematically binding a part of the primary antigen spike protein of the virus to ACE2 and entering the tissues present, which are mainly in the lungs and cardiovascular system (Zheng et. al. 2020).

ACE 2 mechanisms function as receiving the spike protein of Sars Cov 2, SARS-S, and then binding, a second signaling event and conformational change event for cell access of the virus occurs from protein cleavage/priming of a protease(Hirano and Mirikami 2020). Transmembrane acceptance protein protease or specifically, “Type II transmembrane serine proteases TMPRSS2 and HAT can cleave and activate the spike protein (S) of the severe acute respiratory syndrome coronavirus (SARS-CoV) for membrane fusion” (Huerich et. al. 2013). This is done by opening up the antigen embedded in the viral lipid membrane and by opening up ACE2, angiotensin-converting enzyme 2, in similar fashion of the host cell membrane (Huerich et. al. 2013)( Hirano and Mirikami 2020). Because of this feature, it is also potentially of interest to study specific protease inhibitors to manage treatment, “A TMPRSS2 inhibitor approved for clinical use blocked entry and might constitute a treatment option” ( Hirano and Mirikami). Once inside, a COV follows a set of criteria for self-preservation: Conserve genomic organization “with a large replicase gene preceding structural and accessory genes … expression of many non-structural genes by ribosomal frameshifting;… utilize several unique or unusual enzymatic activities encoded within the large replicase–transcriptase polyprotein; and utilize expression of downstream genes by synthesis of 3′ nested sub-genomic mRNAs”(Fehr and Perlman et. al. 2015).

-H-COV integration, and with this specific case in mind, is derived from the RNA in the capsule; “within the helically symmetrical nucleocapsid is the large positive sense, single stranded RNA” where RNA is ejected via motor protein into the cellular matrix of cytoplasm( Malik Ya, 2020). After insertion RNA replication and translation needs to occur on a vast scale before lysing and eruption into mucus can occur properly (Malik Ya, 2020).

“Translation of the replicase gene from the virion genomic RNA and then translation and assembly of the viral replicase complexes is done first; following, replication and subgenomic RNA synthesis, encapsidation occurs resulting in the formation of the mature virus”(Malik Ya, 2020). The “nested” three prime end of the viral RNA contains critical coding information for this all to proceed (Malik Ya, 2020)(Fehr and Perlman et. al. 2015). The major structural proteins are noted as such: “the spike (S), membrane (M), envelope (E) and the nucleocapsid (N) protein” (Malik Ya, 2020). Clarity on the other structural proteins mentioned besides the S protein mentioned above can also be found in Dr. Malik Ya’s review:

“The M protein is the most abundant protein and defines the shape of the viral envelope. The E protein is the smallest of the major structural proteins and participates in viral assembly and budding. The N protein is the only one that binds to the RNA genome and is also involved in viral assembly and budding…”

Once this virus takes out these cells, other infections make use of the issues caused by a lack of lung up keep and excess mucus: Pneumonia is a secondary infection that seems to be one of the more detrimental for example (Lukassen et. al. 2020) (WHO, 2020) (Zheng et. al. 2020). This seems to be why the virus is so deadly, easily expressed, and transported from human-human with such vigor. This is also why a stay at home order, cleanliness, and social distancing is very critical(WHO, 2020).

COVID-19 can be testable in several ways, for rapid test examples, kits for ELISA, and other fast antigen tests are used frequently. Although ELISA tests can show the presence of COV-19 if a patient is in symptomatic stages as a confirmation, the issue lies in the fact that a patient may not contain anti-bodies in their serum for up to ~2 weeks or a week and a half, that are antigen specific (BIORAD, 2020). This usually is not a problem since symptoms arise within the first two weeks; but, in early testing scenarios this is problematic as there might be potential for a false negative. ELISA’s are incredibly fast though and extremely simple to train for and preform for newer medical professionals or volunteers. ELISA well sterility and cleaning procedures is also very critical to mitigate the likely hood of a false positive color change from previous contamination (BIORAD, 2020). Another rapid test is COVID-19 IgG/IgM Rapid Test; “ is a solid phase immunochromatographic assay used in the rapid, qualitative and differential detection of IgG and IgM antibodies to the 2019 novel coronavirus in human whole blood, serum or plasma” (AYTU BIOSCI, 2020). This test is another 2-10 minute rapid test; but is considerably more complex and also requires antigen to be present in serum(AYTU BIOSCI, 2020).

With RT-PCR(Reverse Transcriptase, Polymerase Chain Reaction), it is much safer to fully commit on analysis if swabs are done properly. RT-PCR does not use antigen recognition instead, “RNA molecules are first converted into complementary DNA (cDNA) molecules that can then be amplified by PCR” and conserved targeting sequences(J.L. Jones, 2015). Viruses present in mucus swabs are critical in providing a positive or negative result, swabs from elsewhere with no respiratory mucus present may cause false negatives. These tests can detect influenza viral RNA or nucleic acids in respiratory specimens with high sensitivity and high specificity(conserved gene targets) in 20-30 minutes (CDC, 2019). Following directions for transport of specimen, swab type, and other procedure is critical for real results( CDC,2019). If viruses aren’t being shed, sometimes not enough may be present before symptoms, this test may also create false negatives (CDC, 2019). Another clear fall through with RT- PCR is availability of devices and kits capable of RT-PCR and expenses when compared to the accessibility of other assay(CDC, 2019). In this way, they each server their purpose as being fast, and accessible or being more accurate and still relatively quick. I say relatively because older RT-PCR systems can take multiple days to finish( J. L. Jones, 2015).

Work Cited:

Journal Articles, R.A., and Online Journal Articles:

Adeline, H. et. al. “TMPRSS2 and ADAM17 Cleave ACE2 Differentially and Only Proteolysis by TMPRSS2 Augments Entry Driven by the Severe Acute Respiratory Syndrome Coronavirus Spike Protein.” Dec. 2013. Journal of Virology ,88 (2) 1293-1307. DOI: 10.1128/JVI.02202-13.

Fehr, A. and Perlman, S. et. al. “Coronaviruses: An Overview of Their Replication and Pathogenesis.” 12, February. 2015. Coronaviruses; 1282: 1–23. doi: 10.1007/978-1-4939-2438-7_1

Hoffman, M. et. al. “SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor.”16, April. 2020. CELL. Volume 181, Issue 2, Pages 271-280.e8 https://doi.org/10.1016/j.cell.2020.02.052

Hoffman, M. et. al. “The novel coronavirus 2019 (2019-nCoV) uses the SARS-coronavirus receptor ACE2 and the cellular protease TMPRSS2 for entry into target cells.” 2020. BIORXIV. https://doi.org/10.1101/2020.01.31.929042

Huang, S. et. al. “SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.” 23, April. 2020. Nat Med 26, 681–687. https://doi.org/10.1038/s41591-020-0868-6

J.L. Jones, “On Reverse Transcription PCR”. High Throughput Screening for Food Safety Assessment, 2015. https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/reverse-transcription-polymerase-chain-reaction

Leng, Z. et. al. “Transplantation of ACE2- Mesenchymal Stem Cells Improves the Outcome of Patients with COVID-19 Pneumonia.”2020. Aging and Disease , Vol. 11 Issue (2): 216-228. DOI:10.14336/AD.2020.0228

Leung et. al. “ACE-2 Expression in the Small Airway Epithelia of Smokers and COPD Patients: Implications for COVID-19.” 2020. European Respiratory Journal; DOI: 10.1183/13993003.00688-2020

Lukassen, S. “SARS‐CoV‐2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.” 14, April. 2020. EMBO J e105114. https://doi.org/10.15252/embj.20105114

Prompetchara, E. Ketloy, C. and Palaga, T. “Immune responses in COVID-19 and potential vaccines: Lessons learned from SARS and MERS epidemic.” 2020. Asian Pacific Journal of Allergy and Immunology. R.A. ;38:1-9 DOI 10.12932/AP-200220-0772

Ya, Malik. “Properties of Coronavirus and SARS-CoV-2.” April. 2020. Malays J Pathol ;42(1):3-11. https://www.ncbi.nlm.nih.gov/pubmed/32342926

Xu, H., Zhong, L., Deng, J. et al. “High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa.” 2020. Int J Oral Sci 12, 8 https://doi.org/10.1038/s41368-020-0074-x

Zheng, Y. et. al. “COVID-19 and the cardiovascular system.” 05, March. 2020. Nat Rev Cardiol 17, 259–260. https://doi.org/10.1038/s41569-020-0360-5

Government PDF Sources:

“Coronavirus disease 2019 (COVID-19) Situation Report – 67.” 27, March. 2020. World Health Organization(WHO).

“Modes of transmission of virus causing COVID-19: implications for IPC precaution recommendations.” 27, March. 2020. World Health Organization(WHO).

Website sources:

“Coronavirus Test Update: COVID-19 IgG/IgM Rapid Test.” AYTU BIOSCIENCE. 2020. https://aytubio.com/covid-19/

“Information on Rapid Molecular Assays, RT-PCR, and other Molecular Assays for Diagnosis of Influenza Virus Infection.” CDC. October, 21, 2019. https://www.cdc.gov/flu/professionals/diagnosis/molecular-assays.htm

“What is ELISA?: An Introduction to ELISA.” BIORAD. 2020. https://www.bio-rad-antibodies.com/an-introduction-to-elisa.html

#covid-19 #corona virus

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drlaurynlax · 6 years ago

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Important: How to Find Mold in Your Home or Environment

The tell-tell validation for mold exposure is actually finding remnants in your home, workplace or other environments you frequent. Note: It’s important to realize that many molds are not always seen with the naked eye, nor do they have to infest an entire wall, shower caulking or window sill to be hazardous.

Use “ERMI” Testing

To date, the best initial screening for mold is “surface” or “air PCR” (polymerase chain reaction) testing—also referred to as “ERMI testing.”

The ERMI test was developed by the EPA as a means of determining the relative “moldiness” of a home compared to a group of reference homes that do not have mold. This type of testing involves collecting possible mold samples with a dust cloth kit on surfaces where you suspect mold may be present. The cloth is able to pick up mold DNA on the surfaces of walls, furniture and places it may be, as well as the air. After collection, you or the mold inspector send it back to the lab for testing and mold identification under a microscope.

Some people claim that mold plate testing can work too, but keep in mind, it is not very sensitive. Mold spores differ in weight, density, and air flow characteristics, so some types are more likely to settle on the plates than others. Sticky molds that often appear near water damage, like black mold, may end up evading the plates completely.

Note:

ERMI results will not tell you every single species, crevice or corner of your house that has mold. Results are still limited since they only evaluate the areas where you take a sample. Testing is best to give you an idea of patterns and what types of mold may be present.

There are two primary methods for using ERMI testing:

Hire (the right) professional

DIY

Method 1: Hire the Right Professional

Not all mold inspectors are created equal. Many mold inspectors do not use in-depth, accurate testing methods. If you call up a local mold inspector and ask him to come assess your property, he will probably do a visual inspection and take some air samples, but on their own, they are not enough. Air sampling does not allow identification of particular species.

To accurately assess your exposure, the best practice is to hire a mold inspector who is familiar with proper testing methods (ERMI with EPA validated methods). Do your research. Visit ACAC.org (ACAC is American Council for Accredited Certification) and look for “Certificants” in your area with one or more of the following certifications:

CCIEC, council-certified indoor environmental consultant;

CMI, council-certified microbial inspector;

CMC, consultant

A good mold inspector will walk your property and be aware that the inside and outside can be equally affected; they may ask you questions about your health or timeline of mold exposure; they will know what ERMI testing is; and they may have even heard about Unfortunately, many mold specialists claim they test mold, but do not utilize this in depth type of testing—often missing the lingering root causes of toxic exposure.

Mold Testing Problems

Warning 1: NOT All Mold is Visible

Mold is sneaky and not always seen with the naked eye. Many average mold inspectors, unaware of the nuances of testing, will come to your house, look, and if they don’t see anything visually black on the wall, growing, they may feel you, “You don’t have a mold problem.” Buzz! Mold can still be present. Testing for spore counts can help you see beyond black splotches and green fuzz.

Mold Spore Count Test Guide

0-50 spores – These are only trace levels and are not an issue. Even Stachybotrys is not considered an issue at these levels if the sample does not also contain water markers like Chaetomium and Fusarium or high levels of Penicillium/Aspergillus.

50-200 spores – These are still very low levels. The toxic mold species Stachybotrys and Memnoniella are just about the only species that are considered an issue at this level.

200-500 spores – Up to this point, the most common species (Penicillium/Aspergillus, Cladosporium and Curvularia) are still not an issue and are in the normal range.

500-1500 spores – Sometimes the Penicillium/Aspergillus & Cladosporium levels are in this range and there is not an issue that needs to be remediated. If no water intrusion or mold issue is found during the inspection, these levels can be caused by normal life in an enclosed environment.

1500-3000 spores – This is where the grey area begins. When levels reach this point there may be an issue that needs to be addressed unless there is a corresponding number in the outdoor sample. If no water intrusion or mold issue is found during the inspection these levels can be achieved by a dusty home or A/C system.

3000-10,000 spores – Unless there is a corresponding number in the outdoor sample, this is the point were some remediation may be necessary. If a mold spore source has been identified, then clean up of that area is needed. If there was no water intrusion or mold issue found, the home may need to be cleaned and the duct system should be evaluated.

10,000-25,000 spores – Unless there is a corresponding number in the outdoor sample, a mold spore source has usually been identified and remediation of the area is needed. If there was no water intrusion or mold issue found, the duct system may need to be cleaned and/or a general “Spring Cleaning” of the home.

25,000-75,000+ spores – When spore levels are at this point, a mold issue will be easy to identify. Clean up will be required and should be performed by a Professional Mold Remediator.

75,000-1,000,000+ spores – When spore levels are at this point a mold issue will be evident. Remediation will be required and needs to be performed by a Professional Mold Remediator.

Warning 2: Mold Overgrowth Can Happen in New Buildings

New construction is rarely suspected as a hotbed for mold, but don’t be fooled. Moldy drywall after new construction is particularly common. If you are a mold sensitized individual, consider purchasing an older home with plaster and lath walls instead of wallboard. If that’s not an option, consider mold resistant construction materials. They’re a little more expensive, but worth the price in prevention. Also, whether or not you’re a mold sensitized individual, remember mold can’t grow without water. Therefore, get any problems with leaks, bursts or floods clean, dried and sanitized fast to prevent the problem from happening.

Warning 3: NOT All Mold Testing is Created Equal

The majority of companies and “mold professionals” use air sampling alone; however, air sampling is limited. Not all molds are the same—not all of them float in the air as much. Some are heavier and don’t linger in the air, others are lighter. Some settle out quicker, others slower. Some are dry, wet or sticky. So when an inspector collects just an air sample and results are generated immediately (not the 8 hour+ process), these tests only collect about 5 minutes worth of air, and many people are told “there is no problem”—yet still suffer from symptoms.

Warning 4: NOT All ERMI Testing is Created Equal (Go with a Reputable Company)

The quality of the lab that you use matters. The ERMI test alone is not the end all be all. Some labs try to cut costs by using lower quality primers and probes, leading to missed positives and lower counts of mold than what’s actually present.

Method 2: DIY Mold Test

If a professional is hard to come by, check out the company Mycometrics (Link: https://www.mycometrics.com) for the tools and information for a do-it-yourself mold test at home

The post Important: How to Find Mold in Your Home or Environment appeared first on Meet Dr. Lauryn.

Source/Repost=> https://drlauryn.com/uncategorized/find-mold-home-environment/ ** Dr. Lauryn Lax __Nutrition. Therapy. Functional Medicine ** https://drlauryn.com/

#Holistic #Nutrition #Therapy #Recovery #Functional #Medicine #Dr. Lauryn Lax #Dr.Lauryn

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articlepublication · 2 months ago

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SPRI Bead-Based Purification: Optimizing PCR Cleanup for Superior NGS Results

In molecular biology, precision and purity are everything. Whether you’re prepping for next-generation sequencing (NGS), cloning, or qPCR, the quality of your DNA directly influences your results. PCR cleanup is one of those essential steps that can make or break your downstream applications. And when it comes to PCR cleanup, Solid Phase Reversible Immobilization (SPRI) bead-based purification is the method of choice for researchers worldwide.

SPRI bead-based methods combine efficiency, scalability, and versatility in one simple workflow. They’ve become indispensable for high-throughput labs, small academic teams, and commercial genomic facilities alike. In this article, we’ll explore how SPRI beads work, best practices for optimizing PCR cleanup, and how cost-effective solutions like MagBio Genomics’ HighPrep™ PCR Clean-Up Kit are transforming the purification landscape.

What is SPRI Technology?

SPRI stands for Solid Phase Reversible Immobilization. This method uses paramagnetic beads that bind nucleic acids in the presence of polyethylene glycol (PEG) and salt. The DNA binds to the beads, while unwanted components like salts, primers, enzymes, and nucleotides remain in the solution.

Key benefits include:

Size-selective binding

High recovery rates for DNA fragments ranging from 100 bp to >10 kb

Simple workflows that replace spin-column protocols

Automation-friendly—ideal for 96- and 384-well plates

The Role of PCR Cleanup in NGS Workflows

NGS library preparation involves several steps that require precise DNA handling. After amplification, it’s critical to remove unwanted materials such as leftover primers, free nucleotides, and enzymes. These contaminants can:

Inhibit adapter ligation

Interfere with quantification and normalization

Reduce overall sequencing quality

SPRI beads simplify this process. Add the beads, bind the DNA, wash away the contaminants, and elute clean DNA that’s ready for the next step.

Key Advantages of SPRI-Based PCR Cleanup

1. Efficiency

SPRI workflows reduce the number of steps and eliminate centrifugation. This makes them ideal for high-throughput pipelines and minimizes user error.

2. Scalability

From a handful of samples to thousands, SPRI bead protocols are easily scaled and automation-compatible.

3. Customizability

You can fine-tune your purification with bead-to-sample ratios:

1.8x for total DNA recovery

0.6x to exclude smaller fragments (<300 bp)

0.8x to retain larger DNA for long-read workflows

How to Optimize SPRI Bead-Based PCR Cleanup

Choose the Right Bead-to-Sample Ratio

This is perhaps the most important variable. Use:

1.8x for complete fragment retention

0.8x to remove primer dimers and small artifacts

Avoid Overdrying the Beads

After ethanol washing, beads should be air-dried until no visible liquid remains. Overdrying can make it difficult to elute DNA and decrease recovery.

Use Fresh Ethanol

Old or cold ethanol can decrease the wash efficiency and lead to poor DNA purity.

Elute in the Right Volume

Using 20–50 μL of elution buffer ensures efficient recovery while keeping the DNA concentration high enough for downstream use.

Common Applications in Molecular Biology

SPRI beads aren’t just for NGS. Their versatility spans across:

PCR cleanup: Removing enzymes, primers, dNTPs

Cloning: Preparing inserts for ligation

Sanger sequencing: Enhancing read clarity by removing noise

qPCR: Ensuring template purity for quantification

cDNA synthesis and RNA-seq: Purifying double-stranded cDNA

HighPrep™ PCR Clean-Up Kit: A Reliable SPRI-Based Solution

MagBio Genomics’ HighPrep™ PCR Clean-Up Kit is designed to deliver all the benefits of SPRI bead-based purification at a more accessible cost.

Key Features:

Compatible with all major NGS platforms

Comparable or better recovery than AMPure XP

Validated for manual and automated protocols

Stable shelf life and consistent batch quality

HighPrep™ beads use the same SPRI chemistry as traditional solutions but come at a fraction of the cost, making them perfect for cost-sensitive labs without sacrificing performance.

Automation and High-Throughput Compatibility

As research scales up, automation becomes essential. HighPrep™ PCR Clean-Up is compatible with liquid-handling platforms like:

Thermo Fisher KingFisher Flex

Hamilton Microlab STAR

Tecan Fluent and Evo

These integrations allow genomic core facilities to streamline operations and reduce sample-to-sample variation.

Testimonials and Case Studies

“Switching to HighPrep™ not only cut our cleanup costs in half, but also gave us flexibility with automation. We saw no drop in yield or data quality.” — Senior Scientist, Biotech Startup

“We routinely process 10,000 samples a month, and HighPrep™ has been instrumental in keeping our workflow smooth and cost-effective.” — Genomics Core Facility, UK

Sustainability and Supply Chain Reliability

Unlike spin columns, SPRI beads reduce plastic usage. HighPrep™ PCR Clean-Up is also offered in bulk formats to minimize packaging waste. And unlike some competitors, MagBio ensures consistent availability with a stable supply chain.

Cost Comparison

When to Use SPRI-Based Purification

SPRI bead cleanup is ideal when you need:

Fast and reproducible DNA purification

High throughput compatibility

Flexible size selection for fragment trimming or retention

Reliable performance for sensitive applications like NGS

Final Thoughts

SPRI bead-based purification has changed the game for PCR cleanup and beyond. The simplicity, accuracy, and scalability of this approach make it indispensable in modern molecular biology. With cost-effective and high-performing kits like HighPrep™ PCR Clean-Up from MagBio Genomics, it’s easier than ever to streamline your workflow, reduce costs, and get consistent, high-quality results.

Upgrade Your PCR Cleanup Workflow

Explore the power of SPRI-based purification with the HighPrep™ PCR Clean-Up Kit. It’s the perfect solution for efficient, cost-effective DNA purification in any lab setting.

#health #rna #rna extraction #rna extraction kit manufacturers in usa

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foodandbeverages · 2 years ago

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Food Authenticity Testing Services Market 2023 | Latest Trends, Demand, Growth, Opportunities & Outlook Till 2033

In 2023, the US$ 5893 million for the food authenticity testing service market is estimated to reach a revised size of US$ 10112.8 million by 2033. This market is expected to expand at a CAGR of 6% during the analysis period.

Check the sample report available in PDF format@ https://www.futuremarketinsights.com/reports/sample/rep-gb-12630

Key Takeaways from Market Study

The food authenticity testing service market is expected to grow at a CAGR of 6% over the forecast period.

It is estimated that the food authenticity testing service market in North America will remain strong during the forecast period.

Due to its large population, Asia Pacific is expected to be the fastest-growing region for food authenticity testing services

Testing meat speciation authenticity is bolstered by a high incidence of meat consumption

The food authenticity testing services market in China is expected to witness significant growth at a CAGR of around 4.4%.

Competitive Landscape:

Recent Developments:

SGS announces the opening of its new food testing laboratory in Papua New Guinea. In addition to food safety, quality, and sustainability, SGS offers a comprehensive range of services.

Food Authenticity Testing Services Market Segmentation by Category

By Food Tested:

Meat & Meat Products

Dairy & Dairy Products

Processed Foods

Other Food Tested

By Target Testing:

Meat Speciation

Country of Origin & Ageing

Adulteration

False Labelling

By Technology:

PCR-Based

Liquid Chromatography-Mass Spectrometry (LC-MS)

Isotope

Immunoassay Based/ELISA

Other Technologies

By Region:

North America

Latin America

Europe

The Middle East and Africa

East Asia

Information Source: https://www.futuremarketinsights.com/reports/food-authenticity-market

#Food Authenticity Testing Services Market

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thomasjanson · 4 years ago

Link

AMD Biotech Inc, is a manufacturer of high quality purification products Tucker, GA. The information contained in this site is strictly for the purposes of providing information only. The information presented does not constitute an offer to sell, or the solicitation of any offer to purchase, any securities mentioned herein.

#high quality purification products #purification products

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cqblog319 · 4 years ago

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Manual Viking Madison Model

Viking Manuals Online

Manual Viking Madison Model Mayhem

Manual Viking Madison Model Railroad

Capacitors, Resistors and Schematics

Vintage HAM Radio / CB Radio Schematics & Service Manuals

Download 86 Husqvarna Viking Sewing Machine PDF manuals. User manuals, Husqvarna Viking Sewing Machine Operating guides and Service manuals. . Use this sewing machine only for its intended use as described in this manual. Use only attachments recommended by the manufacturer as contained in this manual. Never operate this sewing machine if it has a damaged cord or plug, if it is not working properly. The legendary Viking range is available in gas and electric models, as well as an array of sizes and finishes to fit any kitchen. And for those who want professional performance without the professional look, there is the Designer Series. Clean lines and contours meld gracefully into cabinets with style, offering a striking new look for Viking.

CB Radio Schematics / Service Manuals

Do you need a HAM / CB / Scanner-Monitor radio schematics / service information to repair, modify, test or install you vintage CB radio / citizens band transceiver? We carry a good selection of schematics / service manuals for vintage CB radios. In most cases the service info consists of: easy-to-read schematic diagrams, receiver alignment instructions, transmitter adjustment & alignment data, troubleshooting tips, repair procedures, 'how it works' explainations, synthesizer frequency tables and parts lists. Please see below for a complete list of the CB (Citizens Band Transceivers) brands we carry info for.

How to Order HAM / CB Service Manuals

Easy…just see if the make and model number of your HAM / Scanner-Momitor/ CB radio is listed below. If it is, we have the CB service information you need. Schematics and Service Information usually runs 12 to 70 or more pages per CB model. Cost of $19 US per CB and includes 1st class postage to anywhere in USA or Canada. Other counties add $4.00 US. Checks, MO/PO, PayPal and 2CheckOut can be sent to:

Dave & Babylyn Cantelon 6 Ferncrest Gate, Scarborough, Ontario, Canada, M1W 1C2.

email: [email protected]

Other JustRadios Services

RESISTORS for Tube Radios Resistor $ Price List Resistor KITS

Favorite Radio Links

Ham, Scanner-Monitor and CB Radio Schematics / Service Manuals is available for these Makes:

... starting with: ABCDEFGHIJKLMNOPQRSTUVWXYZ

MAKE and Model

AIMOR CB-7000 AIRCASTLE JE321 23-02 AIRLINE (See WARDS) ALARON B-1025 B-1050 B-1100 B-1150 (HA-23C) B-4075 B-4900 B-5050A B-5200 Alinco most owners manuals and many service manuals Alliance ALLIED A-2507 (17B6790X) A-2530 (1786825X) A-2533 (17B6440X) A-2543 (17-9801S) A-2544 (17R98025) A-2545 (17B98035) A-2559 (21B5559U) A-2561 (21B5561) A-2564 (17A6966U) A-1567 (17R6435X) A-2568 (17A696SU) A-2569 (17Cb469U) KN2567 ALLSTATE(See SEARS-SILVERTONE) Alpha Ameco AMERICAN ELECTRONICS 76-501 (Spirit) 76-551 (Buccaneer) 76-601 (Freedom) AMERICAN MOTORS 3231850 Ameritron AMPHENOL C-75 80 600, 625 650 675 725 750 777 Amp Supply Antenna Mart APELCO AR-9 AR-10 APOLLO AP704 Midland 78-976 (#175, #180, #259) ARKAY SQ-9/-9Y1 ARVIN 20Y33-19 (1.45501) 20Y55-19 (1.45601) Astartic Astron AUDIOVOX MCB-500 MCB-750 MCB-1000 MCB-2000 MCB-3000 MCB-5000 MDU-6000 Autek AUTOMATIC CBF-2179 CBH-2265 CBR-2175 MCE-6510 MCR-6450 PCR-6452 RED-3335 TRC-6448 TRE-6500 TRl1-6454/A AWA/THORN 1503 Midland 78-976 (#175, #180, #259) B&K PF-1 (488-089-9-001A) B&W Babcock BELTEK Enduro 5 (W5396) Enduro 23 (W5398) BENDIX CB-6/-12 BOMAN CB-515 CB-555 CB-720B CB-725 CB-750 CB-755 CR-770 CD-910 CR-920 CR-930 CB-950 CBH-990 CBM-6100 CBR-9600 CBR-9900 CBR-9940 CBR-9950 Breting BROWNING Baron Brownie Eaglette Eaglette 2 Golden Eagle Mark II (69R, 69T) Golden Eagle Mark III LTD Mark II Series B, SSB-115 R-27, S-23 Sabre SST SST (LP) (Ser. No. T-50000 & up) SST-2 XM-888 CADRE C-75 500/C 510 510A, 515 525 (500-1, 520) CALTRON CB-7500 CAPITOL ND-309 CARDON Iroquois 40 Midland 78-976 (#175, #180, #259) CDE Central Electronics CHANNEL MASTER CB6830 CB6832 CB6834 CB6835 6258 6423 6553 6554 CHRYSLER 4048076, 4048077 4091173 4094176 4094177 4094178 4094179 CITI-FONE C0-5/6/-5/12 CD-5A/6. CD-5A/12 CD6/6, CD6/12 CUII/6, CUII/12 II (1103-01) SS 19 99/6, 99/12 CITIZEN M5 MPL-5 SSB-M6 CLARICON 16-523 15-200 15-410 15-500 15-600 30200 (Intruder) 30400 (Pirate) 30500 (Raider) 30600 (Privateer) 30800 (Activator) CLARION DMA-066 (JC-202E, RCJ-003) JC-201E (PE-620E) JC-201E (RE-366E) JC-201E (RCJ-001) JC-202E (DMA-006, RCJ-003) JC-202E (PE-621E) JC-202E (PE-672E) JC-202E (RE-367E) JC-203E PE-620E PE-621E PE-672E RCJ-001 RCJ-003 (DMA-006, JC-202E) RE-366E RE-367E Sky-Scanner 37500 Clarostat COBRA CAM-88 CAM-89 V 6 19 19GTL 19M 20 21 21GTL, 21LTD 21X 21XLR 23, PAC-V 24 25 25GTL, 25LTD, 25LTD Classic 27 28, PAC-24 28A 29 29GTL, 29LTD, 29LTD Classic 29XLR 32XLR 45XLR 46XLR 47XLR 50XLR 55XLR 63GTL 66GTL 77X 78X (PCB CB-262) 85 86XLR 87GTL 89GTL 89XLR 130 131 132 (EP & LP) 132A 132XLR 134 135 (EP & LP) 135B 135XLR 138/A 138XLR 139 139XLR 140GTL/142GTL 148GTL 148F-GTL: same as #249 without FC 880 1000GTL 2000GTL 2010GTL-WX Collins COLT Excalibur SX33 210 222 290 350 (M-374) 390 480 485 800 1000 1200 COMMANDER 80 (Amphenol 80) 750 (Amphenol 750) 777 (Amphenol 777 COMMANDO 2310 2320 2325 2340 Conar CONCORD TG-132B CONVOY CON-400 CON-430 CON-450 COURIER (E.C.I.) Blazer 40D Cadet 23 Caravelle Caravelle II Caravelle 40D CCT-2 W/CHB-3 CCT-3 CCT-4 Centurion Centurion PLL40 Centurion Plus Centurion 40D Chief 23 Citation Classic Classic II Classic III Classic PLL40 Comet 23 Conqueror Conqueror II Conqueror 40D Cop-Scan Cop Scan VHFHL Courier Clipper 23 Courier Royale Courier TR-5 Courier TR-23S Courier 1M, 23 Courier 23 Plus Cruiser CWT-30 CWT-40 CWT-50 Fleet Courier, 30-B Galaxy Gladiator Gladiator Plus Nightrider 40DR Ranger 23 Rangler 40D Rebel FLL Rebel 23+ Rebel 40A Rebel 234 Redball Renegade 40 Rogue 40 Spartan PLL 40 Spartan Plus Spartan SSB Traveller (ML-100) Traveller II CPI CP400 CRAIG L101 L102 L103 L104 L131 L132 L150 L231 L132 L600 4101 4102 4103 4104 4201 DAK Mark IX Mark V Mark X Cushcraft Antenna manuals DELCO (See GM) DEMCO CH-300 Chalet, DMT-110 Ravelle Ravelle 23 Satellite CB-1A Satellite Deluxe Satellite M-10A, R-101A, T-110A, VW-12-120 Traveller (Series B) DEWALD TR-6/-12/910 Dentron Dowkey Dragon Drake manuals and schematics DUO COM 100 DYNASCAN(See COBRA) ECHO 49’er 99’er EICO 712 (Sentinel 12) 740 760/61W/62/62W 770/W 771W, 772W 777 779 (Sentinel 23) 779A (Sentinel Pro) 7723 7923 (Nova 23) Eddystone Eico Eimac Eldico ELECTRA Bearcat III (various scanner monitors) ELECTRONIC 2000 Contact CB-23CH ELECTRA Jolly Roger ELECTROPHONIC (See ROSS/ELECTROPHONIC) Elmac Electo Voice EVERSONIC HA23C FANON CHB-3 CHB-4 Fanfare 100 Fanfare 100FI Fanfare 120 Fanfare 125F Fanfare 182F Fanfare 184DF Fanfare 185DF Fanfare 185PLL Fanfare 190DF Fanfare 350F Fanfare 700 Fanfare 880 Fanfare 880DF FCB-9 IC-3000 TC-5000 ID-40 L-1000 L-2000 Scanfare Scanfare VHFHL SFT-400 SFT-500 SFT-600 SPT-800A SFT-900 (Guardsman) T-600 T-606 T-700 T-707 T-800 T-808 T-909 T-1000C FIELDMASTER MF-1001 Micro Mini 3 Micro Mini 6 Micro Mini 23 TR-18M FULCOMM 2301/2302 2303 GEM MARINE GB-11935 GR-11930 GEMTRONICS GT-44 GT-55 GT-230 GTX-23 GTX-36 GTX-44 GTX-66 GTX-77 GTX-2300 GTX-2325 GTX-3000 GTX-3313 GTX-4040 GTX-5000 GENERAL ELECTRIC Y7050A 3-5800A 3-5801A 3-5804A 3-5804B 3-5804D 3-5804F 3-5804G 3-5810A 3-5810B 3-5811B 3-5812A 5-5813A 3-5813B 3-5814A 3-5814B 3-5815A 3-5817A 3-5818A 3-5819A 3-5819B 3-5811A 3-5821B 3-5825A 3-5825B 3-5869A 3-5871A 3-5871B 3-5875A 3-5900A 3-5975A GENERAL RADIO and TELEPHONEthe below CB's and more MC-6 Super MC-8 Super MC-9 Super MC-11/A VS-6, VS-7 GLOBE CB-100 CB-100A CB-200 Globe Master 65-210 X-90 (Pocketphone) 65-12B (President VIII) 9000 (18-9000) 9001 (18-9001) Geloso GENERAL MOTORS (GM) CBD-10 CBD-12 CBD-20 CBD-20A CBD-20B CBD-20U GM13B GM23C GM123A GM130 GM130A GM132 70BCB1 70BCB1 (16000570) 70BFMC1/2 70BFMC3 71YFMC1 73AFMC1/2 73AFMC3 76CFMC1/2 76CFMC3 76KFMC1/2 76KFMC3 80AFMCI 80BCB1 (16000570) 80BCB2 80BFPC1 80BFTC1/2 81YFMC1 86CFTC1/2 86WTC1/2 90BCBI 90BCB2 908ECB1 90BETCI 90BFMC1 90BFPC1 90BFTC1, 90BCB1/90ECB1 91YFMC1 96CECB1 96CETCI 96EECB1 96EETC1 96KECB1 96KETC1 4120 4145 4175 4230 994856 995107 995432 7898220 7898340 7898426 78Y8436 7898470 7898530 789B540 7898570 16000440 16000443 16000456 16000466 16000570 (70BCB2) 16000570 (80BCB1) 16000810 16000866 16000876 16001111 16001691 16003560 16006170/180/81 GONSETthe below CB's and more G-11-3303/04/-05, G-12 (3316, 3329) G-14 (3430, 3433) G-15 (3428, 3429) GRAND PRIX D-1325RF 1125 GRANADA CB-6 CB-7 FCB-27 Gross HALLICRAFTERS (the below CB's plus lots of vintage Hallicrafters Ham Gear manuals and schematics BC-8 CB-1 CB-3 CB-3A CB-4 (Littlefone) CB-5A CB-5 Mark II, HA-II, P-5-120 CB-7 CB-8 CB-9 CB-10 CB-12 CB-14/-17 CB-19 CB-20 CB-21 CB-14 PS-20 HALLMARK 511 1250B HAMMARLUNDthe below CB's and more CB-SIX (CB-6) HANDIC 21 32 43C 65C 199 230 235 240 305 605 605DL 2305 3605 Harvey Wells HEATHKIT (HEATH)the below CB's and other Heathkit manuals available for Heathkit HAM gear and Heathkit Test Equipment CB-1 GRS-65A GW-10A/D, GWW-10A/D GW-11A/-11D, GWW-11A/11D GW-12A/-12D, GWW-12A/-12D GW-14, GWA-14-1, GWW-14 GW-14A, GWW-14A/-14A5 GW-21, GWW-21 GW-22A/-22D, GWW-22A/-22D GW-30 GW-31, GWW-31 GW-32A, GWW-32A/D, GW-42. GWW-42 MW-34, MWW-34 Henry Hewlett Packard Hickok HITACHI CM-2315H CM-2380C LM-2400C/H CM-2425H CM-4800C/H HY-GAIN / HIGAINthe below CB's and other VIII (3078) 623, 623A 670, 670A (HyRange I) 670B (HyRange I) 671, 671A (HyRange II) 671B (HyRange II) 672, 672A (HyRange III) 672B (HyRange III) 673, 673A (HyRange IV) 674, 674A (HyRange V) 674B (HyRange V) 675, 675A (HyRange VI) 681 (HyGain I, Ia) 682 (HyRange IIa) 2679 (HyGain 9) 2679A 2680/81 2682 2683 (III) 2701 (I) 2702 (II) 2703 (III) 2705 (HyGain V) 3077 (VII) ITT CB4000M 320 4400M Icom James JANA CB92 KJCB-32MKVI KJCB-91 KJCB-420 KJCB-440 J.C. PENNEY 681-6241 (981-8360) 981-6051 (Golden Pinto) 981-6065 981-6066 981-6067 981-6075 (Pinto SSB) 981-6200 981-6204 (981-7461) 981-6213 (981-0649) 981-6216 (981-3130) 981-6218 (981-8394) 981-6221 (981-8345) 981-6225 (981-8352) 981-6235 (981-0607) 981-6236 981-6237 (981-8352) 981-6240 (981-3445) 981-6147 (981-3080) 981-6255 (981-7701) 985-6050 (Pinto 23) 985-6055 (Pinto Jr.) 985-6060 (Pinto-23B) Pinto (981-6080/81/82/83/84/85) JET SOUNDS CB-7000 J.I.L. 606CB 615CB 851CB 860CB JOHNSONthese CB's and other Johnson manuals Duo-Scan Low Range, High Range 241-0340-001, 241-0340-002 Hi/Lo Duo-Scan, UHF/VHF Duo Scan Messenger II Messenger III Messenger III (241-143) Messenger 40 Messenger 50 Messenger 80 Messenger 92/40 Messenger 100 Messenger 102 Messenger 108 Messenger 109 Messenger 110 Messenger 120 Messenger 120A Messenger 121 Messenger 121A Messenger 122 Messenger 123 (242-0123A and B) Messenger 123 (242-123 Rev. C, D, E, F, G Messenger 123A (242-0123) Messenger 123B Messenger 123SJ Messenger 124 Messenger 124M Messenger 125 Messenger 130 Messenger 130A Messenger 131 Messenger 191 Messenger 223 Messenger 250 Messenger 300, 323 Messenger 320 Messenger 323A Messenger 323M Messenger 350 Messenger 351 Messenger 4120 Messenger 4135 Messenger 4110 Messenger 4145 Messenger 4190 Messenger 4175 Messenger 4230 Messenger 4250 Messenger 4730 Personal Messenger UHF Mono-Scan, VHF Mono-Scan Viking 230 Viking 260, 270 Viking 352 Viking 430 Viking 4330/360 Viking 4740 Viking 242-126, -127, -128, -129 239-0125 (Power Supply) 239-0125 (Rev.) KAAR TR327, 327A, 327B TR336 (Skylark I) TR337 (Skyhawk II) 6-117TR333B, 12-117TR333B, 32/TR333B 12TR335 (Skyhawk) Kenwood Owners Manuals KMART D40 KNIGHT KN-2500 KN-2520, KN-2522 KN-2526 KN-2550, KN-2560 KN-2565 KN-2567 KN-2580 KN-2585 KN-2590 24SC180 KRACO KB-2355 KB-4045 KCB-1300 KCB-1307 KCB-1401 KCB-2310 KCB-2310A KCB-2320A KCB-2320B KCB-2330B KCB-2340 KCB-2370 KCB-2390 KCB-4000 KCB-4001 KCB-4003 KCB-4004 KCB-4005 KCB-4010 KCB-4020 KCB-4030 KCB-4060 KCB-4070 KCB-4088 KCB-4090 KCB-4095 KRIS T-23 (516-123) Valiant Vega (516-139) Ventura Victor (416-123) Victor II (416-124) XL-23 XL-23A XL-25 XL-45 XL-50 XL-70 23+ (516-023) 99’er Knight Kit LAFAYETTE these CB's and more Com-Phone Mark II Com-Phone 23 Com-Phone 23A Comstat 19 Comstat 23 Comstat 23 Mark V Comstat 23 Mark VI Comstat 25A Comstat 25B Comstat 35 Comstat 525 Dyna-Com 2 Dyna-Com 3 Dyna-Com 3A Dyna-Com 3B Dyna-Com 3C Dyna-Com 5 Dyna-Com 5A Dyna-Com 6 Dyna-Com 12 Dyna-Com 12A Dyna-Com 23 Dyna-Com 40 HA-100, HB-111 HA-300B HA-303 HA-310 HA-310A HA-420 HA-450 HB-23 HB-23A HB-115A HB-200 HB-222 HB-333 Series A HB-400 HB-444 HB-444/25A HB-500 HB-501 HB-502A HB-525A, 525B HB-525C HB-525D HB-525E HB-525F HB-550 HB-555 HB-555 (Rev.) HB-600 HB-615 HB-625A HB-640 HB-650 HB-700 HB-740 HB-750 HB-940 HB-950 HB-4000 HE-15 HE-I5B HE-16, HE-18 HE-20AWX HE-20B HE-20C HE-20T HE-20TA HE-20WX/-29 HE-29A HE-90 LM100 LM200 LM300 LM400 Micro-12 Micro-23 Micro-66 Micro 223A Micro-723 Micro-923 Monitorscan-8 (99-26288W, 99-26296W) Publicom 1 Telsat SSB-25 Telsat SSB-25A Telsat SSB-50 Telsat SSB-50A Telsat SSB-75 Telsat SSB-100 Telsat SSB-140 Telsat SSB-80, SSB-120 Telsat 23 Telsat 50 Telsat 150 Telsat 924 Telsat 925 Telsat 1000 Telsat 1023 Telsat 1050 Telsat 1240 LAKE 410 450 600 650 750 (NDI77-591) 5000 (SN-16) 5100 (SN-14) LA SALLE LA-101/101A LA-101-AN LINCOLN L-2542 (17A0766S) LLOYD'S WT-715, 5A26 Lysco MAJOR M360, M588 like #175, #180, #259, #291, etc. MAGNAVOX WT101 MARK PRODUCTS Invader 23 Lancer 23 MASCO MCB-9 Maxon MEDALLION 63-030 63-200 63-240 63-540 METROTEK Bronco (CB-280) Charger (CB-281) Colt 23 Mustang, Pacer Pacer II MESSENGER 202120 121 122 123A 130 250 MIDLAND these CB's, Scanner-Monotors and more 13-130 13-132 13-133B 13-133C 13-133E 13-150 (EP) 13-160 13-700 13-700 (Rev.) 13-700B 13-701 13-701B 13-701B (Rev.) 13-720 13-721 13-722 13-723, 723B 13-724 13-724B 13-725 13-725B 13-727B 13-729 13-762 13-763 13-770 (6 Ch.) 13-770B 13-772 (12 Ch.) 13-775B 13-777 13-777B 13-778 13-779 13-785 13-790 13-795 13-796 13-800 13-801 13-830 13-845 13-852 13-853 13-854 13-855 13-856 13-857 13-857B 13-858 13-861 13-862 13-862B 13-863 13-863B 13-864 13-866 13-867 13-868 13-869 13-870C 13-870D 13-871 13-872 13-873 13-874 13-877 13-878 13-879 13-879B 13-880B 13-881 13-881B 13-882 13-882B 13-882C 13-883 13-883B 13-884 13-885 13-886 13-887 13-888B 13-892 13-893 13-894 13-895 13-896 13-898 13-898B 13-912 Scanner-Monitor 13-914 Scanner-Monitor 13-915 Scanner-Monitor 13-918 Scanner-Monitor 13-922 Scanner-Monitor 13-925H, 13-925L, 13-925M 13-927 Scanner Monitor 13-930 Scanner Monitor 13-950 Scanner-Monitor 13-955 13-976 18-143C 63-445 76-858 76-860 76-863 76-886 77-002 77-003 77-004 77-005 77-101B 77-101C 77-150 77-200 77-821 77-824B 77-824C 77-825 77-830 77-838 77-849 77-856 77-857 77-859 77-861 77-861B 77-882 77-883 77-888 77-889 77-899 77-955 77-963 78-574 78-976 78-999 79-006 79-007 79-891 79-892 79-893 79-900 100M 150M 200M 2001 3001 4001 5001 6001 7001 MJF owners manuals MONITORADIO TG-1 MOPAR (See CHRYSLER) MORROW CB-1/-2/-3 VP-100-4A 5W1-6, -12, -117, 5W-6, -12, -117 MORSE/ELECTROPHONIC CB-700 CB-800 CB-2000 2001 3005 MOTOROLACB's and more CF925AX CM540 CM550 CM555 CR520/521 CT950AX T4000A T4005A T4009A (CB-1135) T4010A T4020A T4022A T4025A (CB-1136) NDI PC-101 PC-102 PC-200 PC-201 NUVOX CB-7000 TC-5020 OLSON CB-88 CB-409 RA-530, RA-690 RA-590 (Sidebander II-Rev.) RA-717 OSBORNE 300 PACE 10-2 Scan 108H, 108L, 108U, 208, 302 Auto-Mate C123A CB76 CB110 CB113 CB115 CB125 CB143 CB144 CB145 CB150 CB155 CB161 CB162 CB166 (23 Ch.) CB166 (40 Ch.) CB166C (40 Ch.) CB185 CB223 CBST-23 (Sidetalk 23) DX2300B P2300 Pace-Mate 2 W/5812 Plus 23 Sidetalk CB-1023 Sidetalk CB-1023B Sidetalk 1000B Sidetalk 1000M (LP) TA2300/B I, II, II-S 100 100ASA 100-5 123, 123A (EP) 123A (LP) 150 133 200 223 1000B 1000M 1000MC 2300 2300 (LP) 2300C 2376 2576A 2376B 5000 8003 8008 8010A 8014 8615A 8016 8041 8046 8047 8092 8093 8113 8117 8155 8193 8340 PAL Coyote 23 Roadrunner 23 PALOMAR Digicom 100 Skipper 71B Skipper 73 SSB-500 (LP) SSB-500 (EP) 21 49 4100 PANASONIC CQ-B4989EU CQ-B5919EU CR-B1717EU CR-B4700EU CR-B47J7EU CR-B4747EU RJ-22 RJ-3050 RJ-3100 RJ-3150 RJ-3200 RJ-3250 RJ-3450 RJ-3600 RJ-3660 RJ-3700 T-1 PEARCE-SIMPSON AC Power Supply Alley Cat 23 Bearcat 23, 23B Bearcat 23C Bengal SSB Bobcat 23 Bobcat 23B Bobcat 23C Bobcat 23D Bobcat 23E Cherokee 8+8 Cheetah SSB Cheyenne 8 (PR-78) Comanche 16 (PR-160) Companion Companion II Companion III Companion IV Cougar 23 Cougar 23 (LP) Cougar 23B Director Director (Rev.) Escort Gladding Hi-Skan Guardian 23 Jaguar 40B Leopard B Lion 40 Lynx 23 Panther Panther SSB Power Match Puma 23 Puma 23B Puma 23C Pussycat 23 Sentry Simba SSB (LP) Super Lynx Super Panther Mk I, Super Bengal Mk I: Super Bengal Mk II Super Tiger 40A Tiger Mark-2 Tiger 23 Tiger 23B Tiger 23C Tiger 40 Tiger 40A Tomcat 23 Tomcat 23B Wildcat Wildcat II PENNCREST / PENNEYS (See J.C. PENNEY) PHILMORE CC-1, -1W, CT-1, -1W, TC-11, -11W, TC-612, -612W PIONEER GT-1100G GT-6600 POLY-COMM PC-2-6/-12 PC-N-6/-12 (4 Ch.) Pro Senior 23 PRESIDENT (same as UNIDEN) Adams Andrew J AR-7 AR-14 AX-14 AR-44 Dwight D (early) Dwight D (1003002) Grant (early) Grant (1005002) Honest Abe James K (1015001) John Q Madison (early) Madison (1010002) McKinley Old Hickory P400 PC-14 Teddy R Thomas J Veep Washington (early) Washington (1001002) Zachary T (early) Zachary T (1002002) RADIO SHACK(See REALISTIC) RADIOCOM 27C-2A/-2B/-2C RADSON RP-115/-612, RT-70A/-75A Racal RAY JEFFERSON CB-505 C8-701 CB-705 CB-707 CR-711 (Saturn) CB-740 CB-845 CB-905 RAYTHEON Raycom Raycom II Raycom III Raycom IV Ray-Tel TWR-1 Ray-Tel TWR-2 Ray-Tel TWR-3 Ray-Tel TWR-4 Ray-Tel TWR-5 TWR-6 TWR-7 TWR-8 TWR-9 TWR-11 RCA CRM-P2A-5 CRM-P2B-5, CRM-P3A-5 Mark VIII (17704) Mark 9 Mark 10 (CB-2601A) 14T100/200 14T260 14T270 14T275 14T300 14T301 14T302 14T303 14T305 14T400 14T405 14T410 REALISTIC Americana 23-Plus (21-023) Patrolman Pro-7 (20-5001) Patrolman Pro-8 (20-162) Patrolman Pro-9 (20-164) Pro-3A (20-452) Pro-7B (20-173) Pro-77B (20-172) TRC-X23A (21-1136) TRC-5 TRC-8D TRC-9 (21-139) TRC-9A (21-139) TRC-l0A (21-126) TRC-11 (21-141) TRC-12 (21-909) TRC-14 (21-032) TRC-15 (21-003) TRC-18 (21-120) TRC-23B (21-128) TRC-23C TRC-24 (21-124) TRC-24A TRC-24B/-24C TRC-27 (94L595) TRC-27A TRC-28 (21-116) TRC-29 (21-135) TRC-30A (21-143 Navaho) TRC-35C (21-117, Rover 1500) TRC-35C (21-117 Rover 1500, Ser. #17380001 and Up, Code 5A5 and Up TRC-40 (21-140) TRC-44 (21-1137) TRC-44B (21-1061 TRC-46 (11-146) TRC-47 (21-147) TRC-48 (21-150) TRC-49 (21-143) (Navaho Pro Niner) TRC-50 (21-136) TRC-50A TRC-50B (21-138) TRC-52 (21-142) TRC-55 (21-151) TRC-56 (21-153) TRC-57 (21-157) TRC-61 (21-161) TRC-66 (21-105) TRC-67 TRC-68 (21-168) TRC-88 (21-188) TRC-99 (21-107) TRC-99C (21-133) TRC-100B (21-134) TRC-101 (21-137) TRC-101B (21-129) TRC-152 (23-Ch. ver. of TRC452) TRC-180 (21-183) TRC-190 (21-185) TRC-201 (21-1501) TRC-200 (21-184) TRC-204 (21-1633) TRC-205 (21-1634) TRC-209 (21-1660) TRC-420/TRC-420-18 TRC-420A (21-1501) TRC-421 (21-1536) TRC-422 (21-1531) TRC-424 (21-1522) TRC-425 TRC-426 (21-1533) TRC-431 (21-1544) TRC-440 (21-1540) TRC-441-18 (21-9440) TRC-448 (21-1561) TRC-449 (21-1562) TRC-450: same as Cobra #219 TRC-452 (21-1521) TRC-454 TRC-455 (21-1542) TRC-456 (21-1523) TRC-457 (21-1580) TRC-458 (21-1581) TRC-459: base ver. of TRC480, #278 TRC-461 (21-1525) TRC-462 (21-1528) TRC-466 (21-1526) TRC-467 (21-1524) TRC-468 (21-1520) TRC-469 (21-1527) TRC-470 (21-1591) TRC-480 (21-1563) TRC-490 (21-1583) REALTONE TR-6134 TR-6436 REGENCY- Lots of CB's and Scanner-Monitors CB-27 CB-501 CB-701 CBM27-6/-12 CR-123 CR-123B CR-142 CR-185 CR-186 CR-202 CR-230 CR-240 Formula/23 (CB-292) GT-523 (CB-283) Imperial (CB-253) Imperial II (CB-254) MT-15S Range Gain Range Gain II Ranger CB270 R1HT1-1, R1LT1-1, R1UT1-1 R2HT1-1, R2LT1-1, R2UT1-1 TME-8H, TME-8H/LH, TME-8H/LL, TME-8H/LM TME-8H/LH/U, TME-8H/LL/U, TME-8H/LM/U, TME-8H/U, TME-8LHM TME-16U (scanner monitors) TME-16H/L, TME-16H/LH/U, TME-16H/LL/U, TME-16H/LM/U TMR-1H, TMR-1L, TMR-4H, TMR-4L, TMR-8H, TMR-8L TMR-1H, TMR-ILH, TMR-1LL, TMR-1LM, TMR-4H, TMR-4LH, TMR-4LL, TMR-4LM, TMR-8H, TMR-8LH, TMR-8LL, TMR-8LM, TMR-12H, TMR-12LH, TMR-12LL, TMR-12LM TMR-8H/LH, TMR-8H/LL, TMR-8H/LM Romper (A-3) Sprint/23 (CB-291) Sprint/23 II (CB-293) 500 (CB-282) ROBYN AM-500D BB-123 DG-30 DG-130D GT-VIIB GT-410D J-123 K-123 LB-23 LB-23A LB-120 R-15 R-25 SB-505D (EP) SB505D (LP) SB-510D SB-520D SB-540D SS-747B SX-101, SX-102 SX-401 SX-402D T-123 T-240D TR-123B TR-123C WV-23 WV-23A WV-110 XL-One XL-Two 007-140 440 605 ROMAR Act-1914 (Code 15206) CB-7000 (Code 15210) ROSS RE-025, RE-050 ROSS/ELECTROPHONIC CB-1000 ROYCE 1-400 1-402 1-406 1-408 1-581 1-590A 1-600A, 1-600B 1-601 (PLL Version) 1-602A 1-603 1-604, 1-604A 1-605A 1-606 1-608 1-612 1-617 1-620 1-621 1-624 1-625 1-630 1-631 1-632 1-635 1-640 1-641 1-648 1-650 1-653B 1-655 1-658 1-660 1-662 1-673 1-675 1-678 1-680 1-682 582 604 607 608 609 611 613 619 651 RYSTL CB-523 CBR-1700 CBR-1800 SAMPSON M10, R101A/102, T110A, VM12-120 SANKYO SCS-555 SANYO TA-2000 TA-4000 TA-6000 SBE Land Command LCB8 Land Command LCM5 Land Command LCM8 Land Command LCM8P SBE-1CB (Coronado) SBE-1SM, SBE-2SM, SBE-3SM, SBE-7SM (Sentinel I, II, III, VII) SBE-2CB (Capri) SBE-3CB (Cascade) SBE-5CB (Cascade II) SBE-6CB SBE-7CB (Sierra) SBE-8CB (Console) SBE-9CB (Catalina) SBE-10CB (Coronado II) SBE-11CB (Trinidad) SBE-12CB (Sidebander II) SBE-14CB (Super Console) SBE-15CB (Cascade III) SBE-16CB (Console II) SBE-18CB (Sidebander III) SBE-21CB (Cortez) SBE-22CB (Catalina II) SBE-23CB (Capri II) SBE-24CB (Shasta III) SBE-25CB (Shasta II) SBE-26CB (Formula D-23 Ch.) SBE-26CB/A (Formula D-40 Ch.) SBE-29CB (Catalina III, Malibu) SBE-30CB (Trinidad II) SBE-31CB (Shasta I) SBE-32CB (Formula D Touch/Com) SBE-34CB (Brute) SBE-39CB (Sidebander V) SBE-41CB (Aspen) SBE-42CB (Cortez 40) SBE-43CB (Touch/Com 40) SBE-44CB (Malibu 40) SBE-45CB (Trinidad III) SBE-47CB (Stowaway) SBE-49CB (Tahoe 40) SBE-54CB (Key/Com 1000) SBE Console VI SEARS, SEARS Silvertone 60006 Midland 78-976 (#175, #180, #259) 6469 (Ch.787.10070) 6471 (Ch.787.10080) 6550 (Ch.549.30500) 6552 (Ch.549.65520), 6553 6554 (Ch.549.30001) 6556 (Ch.789.10010) 6558 (Ch.789.10010) 6562 (Ch. 789.10040/41), 6563 (Ch.789.10050) 7203 (Ch.562.30010) 7204 (Ch.562.30020) 7531 (Ch.789.10080) 7535 (Ch.789.10090) 242.381607 280.6267 370.38050700 (Roadtalker) 562.382007 562.38220700 663.380009 663.380208 663.380707 934.367105 934.367405 934.367705 934.367715 934.367726 934.380607 934.380627 934.380807 934.380817 934.381107 934.381207 934.382607 934.382707 934.383107 SHAKESPEARE GBS-240 GBS1500 GBS2000 (MCB-22) GBS2500 SHARP CB-500U, 500UB CB-700 CB-750A, CB-800A CB-800 CB-800A CB-2170 CB-2260 CB-2460 CB-4370 CB-4470 CB-4670 CB-5470 CBT-58 SILTRONIXCB's and more Apache (AM-1) Cherokee Mohawk (AM-2) SSB-23 SSB-23A (Albatross) SONAR E E (Rev.) FR-2512, FR-2513 FS-23 FS-3023 G H J-23 RF-104, RT-105 RF-2516, RF-2517 RF-2525, RF-2526, RF-2528 T-2 T-6 SONICO CT193 SONY CB-901 SPOKESMAN 700 SQUIRES-SANDERS Admiral Skipper S5S 23’er SSBCO(See MARK PRODUCTS) STAG 357 Standard STANDARD COMMUNICATIONS Horizon 29 (23-Ch.PLL) Horizon 29A (40-Ch. PLL) STEREOSONIC 2300 2355 2360 SUPERSCOPE Aircommand-CB140 Aircommand-CB340 Aircommand-CB640 Aircommand-CBB1040 Aircommand-CBR40 SURVEYOR 1000 1250 1500 2100 2300 2400 2600 2610 2620 2630 Swan vintage radio manuals TEABERRY Big T Five by Five Mighty T Mini T Mini T II Racer T (23 Ch.) Racer T (40 Ch.) Ranger T #219. Scan T Stalker I Stalker II Stalker III Stalker IV Stalker V Stalker VIII Stalker IX Stalker XII Stalker XV Stalker XX Stalker 101, 102, 202 T (23 Ch.) T (4011/40 Ch.) T Bear (4004) T Charlie (4010) T Charlie One T Command (4007) T Control T Dispatch T Scan T Scout Tele T Titan T (4005) Twin T Telcraft TELECON TMC-206 TELEDEX TE6000 Midland 78-976 (#175, #180, #259) TENNA A0902 10901 10902 11302 TENNELEC Tennelec I/II/III Tmc TRAM / DIAMOND Corsair 464 D12 D42 D64 D80, D300 D201, D201A Diamond 40 Diamond 60 Titan Titan II (5320) Titan IIA Titan III Titan IV TR-27D, TR-27E Tram IV XL XL-5 XL-100 Trio Triplet TRIUMPH TC-900A TRS CHALLENGER 460 600 730 850 1200 1400 TRUETONE CYJ4732A-77 CYJ4832A-87 CYJ4834A-87 CYJ4837A-87 CYJ4862A-87 DC4530 (LP) DC4672 DX4370 MCC4370A-57 MCC4434A-57 MCC4434B-67 MCC4532A-47 MCC4531A-57 MCC4620A-67 MCC4630A-67 MCC4635A-67 MCC4720A-77 MCC4724A-77 MCC4760A-67 MCC4770 (DC4710) MCC4774 (DC4774) MIC4322A-37 MIC4350A-37 MIC4434A-67 MIC4512A-47 MCC4622A-67 MIC4726A-67 MIC4731A-67 MIC4733A-67 MIC4737A-67 MIC4739A-67 MIC4812B-17 MIC4820A-86 MIC4821A-86 MIC4920A-96 MIC4920B-37 MID4820A-86 S12 Series B (DX4101) 1250A 1250B UNIDEN (same as late PRESIDENT) Grant, Grant XL Madison PC76XL, PC76XLW Uniden Trucker PTC104 Washington UNIMETRICS Dolphone Digi Scan 4+4 Scanner Monotor) Digi Scan-8 Scanner-Monitor) Dura Scan-4 Dura Scan-8 Mako-1 Marlin-1 Porpoise-1 Sea Horse-1 Stingray-II UNIVERSE 5600 Midland 78-976 (#175, #180, #259) U.S.L. TR-800 UTAC Micro Mini 23 Studio-4000 Super Tiny 23 TR-18M TRX-30 TRX-400 TRX-500 TRX-2000 UTICA MC-27 T and C II T and C III Veb VECTOR IV IX VI X 770 790 VOCALINE ED-27-6, -12, -27M-6, -12 ED-276/-278 JRC-400/-425 PT-27 WARDS GAS587 GEN-680A GEN-696A GEN-702A GEN-716A GEN-719A GEN-730A GEN-774A GEN-774A (Ser. #06801 and higher) GEN-775A GEN-818A Waters WEBCOR ET-350 Weston World Radio Labs WWII/Army/Navy/Airforce Surplus Receiver / Transmitter Schematics We have lots at $5 each. Most models start with ARC, BC or SCR WEBSTER Bandspanner 412 Four-Eleven WT-2 440 550 Yaesu XTAL XCB-4 XCB-5 XCB-6 XCB-7 XCB-11 XCB-12 XCB-23A XCB-28 XCB-88 XSSB-10 XS5B-10 ZODIAC M-5023 M-5026

Viking Manuals Online

Manual Viking Madison Model Mayhem

See Brands and Model/Chassis numbers .

About JustRadios:We are David and Babylyn and we enjoy collecting and restoring vintage radios. We recommend anyone interested in old receivers join a local vintage radio club as well as a national club such as the Antique Wireless Association. We are members of the AWA and several local clubs, namely: Michigan, London, Ottawa and Toronto. You will meet a lot of very knowledgeable collectors and quite often will find that HAM or CB radio / citizens band transceivers part that you thought you would never find. To help out fellow collectors with their repairs we offer a schematics and circuit diagram service for old tube and early transistor radios. Disclaimer: We are not affiliated with Sams Technical Publishing. Trademarks of Sams Technical Publishing include: Sams, the Sams Logo, Photofact, Howard W Sams, Circuit Trace, Grid Trace, Computerfacts, VCRfacts, and Sams Technical Publishing. We are in no way related to, or affiliated with Sams.

Manual Viking Madison Model Railroad

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priyacmi · 5 years ago

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DNA and RNA Sample Preparation Market – Insights

DNA and RNA sample preparation provides accessibility to nucleic acids in a natural form and removes unwanted contaminants. DNA and RNA sample preparation process has become highly refined and multi-national companies are focusing on faster and accurate products. Market players are focused on launching automated workstations to enable faster, refined, and consistent DNA and RNA extraction. These workstations are compatible with various kits, reagents and lab ware.

The global DNA and RNA sample preparation market is estimated to account for US$ 1,541.3 Mn in terms of value in 2018 and is expected to reach US$ 2,327.7 Mn by the end of 2027.

Global DNA and RNA Sample Preparation Market: Drivers

Increasing funding in life science research is expected to boost growth of the global DNA and RNA sample preparation market over the forecast period. For instance, in February 2020, Purdue-affiliated Amplified Sciences LLC and Brightlamp Inc. received approvals for up to US$ 250,000 each from the Purdue Foundry Investment Fund for R&D in life science technologies.

Moreover, collaborative efforts and initiatives to identify therapies for difficult-to-treat illnesses is also expected to aid in growth of the market. For instance, in February 2020, Cellectricon (Molndal), a Sweden–based pharmaceutical company collaborated with StressMarq Biosciences, a Canada-based life sciences reagents supplier, for R&D in neurodegenerative diseases.

North America region held dominant position in the global DNA and RNA sample preparation market in 2018, accounting for 40.6% share in terms of value, followed by Europe.

Global DNA and RNA Sample Preparation Market: Restraints

High cost of products and low investments are expected to hinder growth of the global DNA and RNA sample preparation market. Researches in the life science sector are complex and have uncertainties in terms results. This in turn increases R&D and product cost and deters users from investing in such expensive products.

Moreover, challenges in handling RNA are also expected to limit growth of the market. The presence of RNases is a major challenge in isolating, manipulating, or analyzing RNA. RNases are extremely stable and very active nucleases capable of degrading the RNA.

Global DNA and RNA Sample Preparation Market: Opportunities

Using quality control tools to obtain information on nucleic acid quantification, integrity, and sizing of fragments in next-generation sequencing is expected to offer lucrative growth opportunities for players in the market. Quality control tools can aid in maintaining clean samples that are free from contaminants.

Moreover, market players can also focus on digitalization in the life science sector through adoption of technologies such as wearable devices and artificial intelligence. Digital technology can be used for routine tasks such regulatory filings.

DNA sample preparation segment in the global DNA and RNA sample preparation market was valued at US$ 570.9 Mn in 2018 and is expected to reach US$ 932.0 Mn by 2027 at a CAGR of 5.5% during the forecast period.

Market Trends/Key Takeaways

The market is witnessing emergence of new field of scientific investigation in the form of RNA interference (RNAi). It is a biological process targeted mRNA molecule are neutralized to inhibit gene expression or translation in RNA molecules.

Major companies are focused on R&D in COVID-19 virus. For instance, in February 2020, Covaris, Inc. launched two new kits for viral RNA extraction from nasal or throat swab sample collection devices that include truXTRAC Viral RNA Extraction kit using Puritan Swabs and truXTRAC-PCR Direct Viral RNA Extraction kit.

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Global DNA and RNA Sample Preparation Market: Competitive Landscape

Major players operating in the global DNA and RNA sample preparation market include, Agilent Technologies, Inc., Becton, Dickinson and Company, Bio-Rad Laboratories Inc., DiaSorin S.p.A, F. Hoffmann-La Roche, Miroculus, Inc., Illumina, Inc., PerkinElmer, Inc., QIAGEN, Sigma Aldrich Corp., Tecan Group AG, and Thermo Fisher Scientific, Inc.

Global DNA and RNA Sample Preparation Market: Key Developments

Major players in the market are focused on launching new products to expand their product portfolio. For instance, in February 2020, Miroculus, Inc. launched Miro Canvas, a system that can simplify, automate, and miniaturize complex protocols such as next generation sequencing library preparation, at the Advances in Genome Biology and Technology conference held in the U.S.

Major players in the market are also focused on adopting collaboration strategies to expand their product portfolio. For instance, in March 2020, Fluidigm Corporation, a provider of microfluidics technology, partnered with Next Gen Diagnostics, a provider of automation in pathogen bioinformatics, under which the later will use the Fluidigm Juno system for pathogen whole genome sequencing sample preparation in exchange of milestone payments starting in 2020 and additional revenue to Fluidigm Corp.

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Source https://www.coherentmarketinsights.com/market-insight/dna-and-rna-sample-preparation-market-3620

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nickyhants · 6 years ago

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gateruner · 8 years ago

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So the first two weeks are almost over at my new job - and the verdict is....

I like it! I mean, overall I do. There are some issues, but no workplace is ever perfect and if you go into believing that then it’s just a set up for failure.

This hospital has had some issues though, and it’s hard to know exactly what’s gone down. I have heard various stories ranging from “they wouldn’t give us raises so a lot of people quit” to a surprise visit from The Joint Commission (TJC) that didn’t end very well and several people “retired” or “moved on” to other jobs.

I tend to believe the later is more accurate. Simply because the majority of the full-time staff that are there have been there less than a year. Even the lab manager has only been there 9 months. A few “old timers” with 20 years or so and then the rest of the staff under a year.

That is a strange thing, I must admit. But whatever.....

So here’s my pros and cons list:

Pros -

I get to work in microbiology again! Oh how I missed you! (not reading plates but setups, kit tests, PCR, gram stains, blood cultures, etc! I love my micro!)

I wanted Blood Bank experience, I got it! In spades! That is one busy BB! I have done more crossmatches, DAT’s, cord blood verifications, FMH screens/ issuing Rhogam, etc in the past two weeks than well ever! And it’s all tube testing baby! No gel cards! Woot!

I get more hematology experience! The hospital has an onsite dialysis center as well as a cancer center. That means more “abnormal” differentials than I’ve gotten to see since school.

As part of the hematology and a bit of micro, I get to work with body fluids and cell counts. Again, haven’t gotten the chance since school and clinicals so yay! (first day in micro I saw a joint fluid aspiration that was slam full of gout and pseudo gout crystals! So very cool. So very painful for the patient. Ouch!)

Cons -

No Chemistry. I don’t know if this is a con so much as just the way it is. I’m not really all that upset about it as they don’t do much in the way of special chemistry anyway. And it’s more about knowing how to operate the analyzer as it is anything else. So, eh. Not a big con though.

Some staff are very negative towards the travel techs. That’s not all that unusual but it’s frustrating. No one has been outright ugly to me about it but you hear things. Whispers and mumbled breaths about how the hospital needs to take care of their own first, etc. I did have one girl outright ask what I made. I asked what she made and she walked away. And I was speaking with another tech who had been working a lot of overttime and I said I bet her check would be nice and she said, “yea but not as good as yours though.” Just.... whatever. I have to let it go. Sour grapes? I don’t know. I can sympathize to an extent. It is upsetting when you are a fulltime employee and a part timer comes in making more. I get that. But being petty doesn’t help anyone.

The daytime hematology tech. Ugh. Just ugh. All I can say is thank god I don’t have to work with him anymore. He “trained” me (I use that term loosely, as he didn’t do much training). He was the most negative person as well. And then he tried to say I turned out a manual differential wrong and “punished” me by making me go read out 10 or more diffs and report them back to him. After I found a cell that was almost identical to what I reported on the earlier slide I showed it to him and said it’s what I saw and asked if that was a myelocyte. He said yes and I said again it’s what I saw. Then he said, “oh well maybe you did. I just didn’t see one on my slide.” Are you freaking kidding me?! Ugh. Asshole. But again.... no more workie with him.

Being tossed into the fire. Tonight I was left alone for almost 2 hours with only another travel tech that came on board same time I did. Not cool. And the shift supervisor left an hour and half early. So this may be a sign of things to come. But I can only do what I can do.

There are some bad work ethics in that place. One of them is that an hour before your shift ends, you stop processing any specimens unless it’s stat. I’ve had several people tell me this and it bothers me. Maybe that’s just my own personal work ethic here but I don’t like that. I am not leaving a bunch of work for someone else to come in and clean up. Now tonight I had to. But it was because I was extremely busy. I had two blood cultures go positive (which are extremely Stat and have to be reported out to the doctor within 30 minutes) and lots of specimens from the ER coming in. So several cultures didn’t get set up on plates. It was a matter of priority and I never had the time. But if I had, I certainly would have done it. (Another note is that I had to leave. Literally. I got the impression that if I hadn’t said my shift was over they would have let me keep working and not cared)

But again, overall I love it! I am tired after every shift but I am happy (save for the two days with that one guy).

So YAY for the travel job! First week I got almost 50 hours though! So my first paycheck was today and it was SWEET! Will have about 45 hours this week.

And another random note: My schedule is not what I first thought it would be. I’m 2nd shift (3:00 PM to 11:30 PM M-F, working every 3rd weekend) Blah. So I will have to watch my H50 and other shows after they air. Oh well, no commercials!

#life of a mlt #travel tech #travel jobs #mlt jobs #lab tech

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