#DNAExtraction

[mpbiomedicals]

Unlock Genetic Secrets with MP Bio's DNA Extraction Kit

Explore the essence of life! Elevate your research with MP Bio's Genomic DNA Extraction Kit. Unleash genetic potential effortlessly. Order now!

[usandtheocean]

How I feel about the fact that I can finally start working and volunteering in the lab since my Bridging Visa A has come into effect. Yipee, let me get my hands on some coral. I’m very lucky for my potential PhD supervisors to have put me in touch with a PhD student working on coral microbiology. I’m super keen to get stuck in and help out with DNA extractions, PCR, coral husbandry and whatever other tasks are required.

[coderegenesisbiosciences-blog]

Extremely Precise DNA Extraction for Healthy, Beautiful Smiles

[nrigroupbpl]

NRI Group of Institutions - One Day Workshop on DNA EXTRACTION

NVISMT is organizing a one day workshop on DNA EXTRACTION on the topic "DNA Extraction from different biological materials is using phenol chloroform method" on January 4, 2020. Academicians, research scholars and students of all colleges are invited to register themselves for this upcoming seminar. Registration is available on first come first serve basis.

[themakerstudio]

View this post on Instagram

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DNA extraction in action! #joinallofus #dnaofinstagram #dnaextraction

[katechol]

Day 6/100: Accidentally discarding the elution tube! This is a nightmare of mine that luckily hasn't happened yet. What HAS happened? My first day in the lab, I forgot to add ethanol to the wash buffer and eluted my sample during washing. So I guess it has happened. :S #100daychallenge #100daysofdrawing #achievementunlocked #illustrator #sciencefail #sciencewins #lablife #sciencelife #dnaextraction #slowmofall #wilhelmscream

[viralphd]

Am I the only one who's afraid of unbalanced centrifuges? I'm always afraid if this thing isn't properly balanced it will jump across the room and take half the building with it! 😳😫 . . . . . . . . . . #fearofcentrifuges #scientislife dnaprep #dna #gigaprep #bacteria #laboratory #qiagen #luriabroth #lbmedia #flask #ecoli #dh5alpha #centrifuge #scientist #sciencing #virologist #vaccine #science #bateriology #hepatitiscresearch #medicalresearch #dnaisolation #dnaextraction #molecularbiology #genetics #phd #phdstudentlife #balance #viralphd

[edun]

@emilydunlay - @23andme update! Unfortunately my first sample I sent in was unable to be processed but @23andme is sending me a new replacement kit & the process will start again! :) stay tuned for updates! #adopted #23andme #pathtoselfdiscovery #dnaextraction #dna #ancestry #history

[evoldir]

Fwd: Postdoc: AMNH_BrighamYoungU.2.InsectPhylogeny

Begin forwarded message: > From: evoldir@web.rhpcs.mcmaster.ca > Subject: Postdoc: AMNH_BrighamYoungU.2.InsectPhylogeny > Date: 4 September 2021 at 06:10:17 BST > To: rdmpage@gmail.com > Reply-To: > > > > > We are hiring two postdocs, one at the AMNH and one at BYU as part > of the collaborative GEODE grant (https://ift.tt/3lLsoGa)! > The postdocs would work together with PIs from these institutions > as well as Naturalis, U Alabama and U Florida. Do you like > dragonflies? genomics? morphology? geographical distributions? We > have a lot of exciting work ahead, please join us! See job > ads below, and follow the links to apply:AMNH #postdocjobs: > https://ift.tt/39peS51 BYU #postdocjobs: > > Questions? Email seth.bybee@gmail.com, > jware@amnh.org,paul_frandsen@byu.edu > > Position Title > > Postdoctoral Fellow > > Department > > Invertebrate Zoology - 011 > > Position Summary > > Jessica Ware and team are seeking a Postdoctoral Fellow for anNSFfunded > project focused on a global phylogeny of dragonflies and damselflies > (Odonata). This project is funded across multiple lab groups and > institutions (Ware Lab American Museum of Natural History, Bybee > and Frandsen LabsBYU, Abbott Lab University of Alabama, Guralnick Lab > University of Florida and Kalkman Lab Naturalis), with the goal of using > anchored hybrid enrichment (AHE) to sequence ~70% of all species and > provide a rich set of ecological data layers that can then be explored > in a phylogenetic context. We seek a highly motivated individual with a > bioinformatic skill set to help reconstruct the phylogeny of dragonflies > (Anisoptera) and the broader Odonata fromAHEdata. > > The Postdoctoral Fellow will assist in reconstructing the phylogeny of > dragonflies (Anisoptera) and the broader Odonata using generatedAHEdata, > morphological and other data.  This work is part of a collaborative team > effort to reconstruct the evolutionary history of taxa across Odonata, > and the Postdoctoral Fellow will focus on Anisoptera taxon sampling. > > The postdoc would have the opportunity to mentor undergraduate students > through theAMNHREUprogram, as well as graduate students working directly > on theGEODEproject. There is also the opportunity for the postdoc to > develop their own research. > > Job responsibilities include, but are not limited to: > > DNAextraction, preparation of plates of extractions for shipment > to external sequencing company, post sequencing data processing, > generation of datasets fromAHEpipeline, phylogenetic reconstruction, other > analyses.Hosting workshops to train others in basic evolutionary analyses, > coordinating workshops on morphological data capture, co-mentoring > graduate and undergraduate students.Writing of scientific manuscripts, > presenting data at conferences, outreach activities. > > Required Qualifications > > A PhD (ABDcandidates are welcome).Experience in phylogenetic > analysis, preferably using high throughput sequencing data.A > knowledge and experience of working in a high performance computing > environment.Scripting experience in a coding language like Python or > R.An ability or willingness to work well with others and mentor both > graduate and undergraduate students.Ability to coordinate with other > institutions in data sharing and analyses. > > Preferred Qualifications > > A knowledge of Odonata and/or insects in general.Previous laboratory > and fieldwork skills. > > Physical Demands > > Category > > Full-Time/Term > > Total Number of Scheduled Hours Per Pay Period > > 70 > > Union Status > > Non-Union > > FLSA > > Exempt > > EEO Statement > > The American Museum of Natural History is an Equal Opportunity/Affirmative > Action Employer. The Museum does not discriminate with respect to > employment, or admission or access to Museum facilities, programs or > activities on the basis of race, creed, color, religion, age, disability, > marital status, partnership status, gender, sex, sexual orientation, > gender identity, gender expression, genetic information, pregnancy, > alienage or citizenship status, current or former participation in the > uniformed services, status as a veteran, or national or ethnic origin, > or on account of any other basis prohibited by applicable City, State, > or Federal law. Additional protections are afforded in employment based > on arrest or conviction record, status as a victim of domestic violence, > stalking and sex offenses, unemployment status, and credit history, > in each case to the extent provided by law. If special accommodations > are needed in applying for a position, please call the Office of Human > Resources. > > Quick Link > > https://ift.tt/39peS51 > > Job Title: Post-doc specializing in global phylogeny of dragonflies > and damselflies > > Job Classification: Post-Doc Researcher, non-CFS position > > Posting close date:10/15/2021 > > Start date of this position: > > 01/02/2022; may result in earlier start date > > Required Degree: > > PhD;Required degree must be completed by the start date.ABD candidates > will be considered if their degree will be completedby the start date. > > Experience: > > -Experience in phylogenetic analysis, preferably using high throughput > sequencing data > > -A knowledge and experience of working in a high performance computing > environment > > -Scripting experience in a coding language like Python or R > > -An ability or willingness to work well with others and mentor both > graduate and undergraduate students. > > -Coordinate with other institutions in data sharing and analyses. > > Also desirable, but not essential: > > -a knowledge of Odonata and/or insects in general > > -most publications from graduate work near submission > > -well organized > > -laboratory skills > > -fieldwork skills > > -bowstaff skills > > Duties/Expectations: > > We seek a highly motivated individual with a bioinformatic skill set > to help reconstruct the phylogeny of damselflies (Zygoptera) and the > broader Odonata from AHE data. > > Position is for 1 year with possible renewal for a second year through > the life of the grant. > > Information required at the time of application –Please list the > individual contact information for each of your three recommenders on > the faculty application. At some point during the selection process they > may be contacted to submit their letters of reference electronically > > Document(s) required at the time of application -Please attach your > updated Curriculum Vitae and cover letter to the faculty application. > > Equal Opportunity Employer: m/f/Vets/Disability > > Brigham Young University is an equal opportunity employer. All faculty > are required to abide by the university’s Honor Code and Dress &Grooming > Standards. > > Jessica Ware > via IFTTT

[nickyhants]

Watch the short video on our website (link in bio and then scroll down to "read about what's new at Promega UK" where you will see the video Benchtop Automated Nucleic Acid Purification at The Francis Crick Institute) to see Dr Jayamalini Holding-Pillai and Dr Yan Tsou from @thefranciscrickinstitute describe their work and how the Maxwell RSC instrument is used for automating nucleic acid purification when authenticating cell lines. . . . . #labchat #labwork #scientists #nucleicacid #labwares #lablife #FrancisCrick #TheCrick #TheFrancisCrickInstitute #PhDChat #benchtopautomation #dnaextraction #dna #biologists #biology #science #PhDLife #laboratorywork https://ift.tt/304wdLb

[kemvid]

Alaska city moose count relies on tips, DNA-extracting darts - http://bit.ly/2tmIAFT - #Alaska, #City, #Count, #Darts, #DNAextracting, #Moose, #News, #Relies, #Technology, #Tips

[rotatingbody]

DNA extraction involves Centrifugation

GENES-DNA-RNA-GENETICS-SEQUENCING-RECOMBINANT… sounds familiar? Maybe you already heard some of these terms in one of your science subjects. But have you ever tried extracting DNA from yourself or from any other biological matter? Yes, it is possible! One can isolate DNA from any living matter by just following some steps. But first, let me give you a brief description about DNA extraction and its importance in research then the procedure will follow.

What is DNA extraction?

DNA Extraction is a procedure of removing deoxyribonucleic acid (DNA) from the nucleus of the cells or viruses.

Importance of DNA extraction:

DNA extraction is done usually for diagnostic purposes such as in research and forensics. It is used for the detection of bacteria and viruses. Furthermore, it is even used for identification, characterization and phylogenetic analysis of plants, insects, animals and many other biological matter. In medical field, it plays an important role in diagnosing diseases and other genetic disorders.

Procedure:

1. Cell lysis of sample to release the DNA

Cell lysis means breaking of cells to open so that all components will be released on the solution. This is usually done by grinding or vortexing.

2. Separating DNA from proteins and other cellular debris

Upon cell lysis, all other components will be floating on the solution; hence, DNA which is the product of interest, must be separated from other components. This can be done by various methods. Sample can be filtered to remove cellular debris. Protease, a protein enzyme, can be added on the solution to degrade proteins and other cellular proteins present on it.

3. Precipitating the DNA with an alcohol, then centrifugation

Once the DNA is separated from other components, precipitating it is the next step. This is done by the addition of either ethanol or isopropanol on the solution. DNA is insoluble in the presence of salt and alcohol, so with gentle mixing of the solution, a precipitate will be visible on the solution. DNA can be pelleted-out through centrifugation.

4. Purify the DNA

After pelleting out the DNA, washing and cleaning of pellet is necessary. This is done in order to further remove any remaining contaminants or residues of the pelleted DNA. Purification of DNA is done by resuspending it on a slightly alkaline buffer.

 5. Confirmation of the presence and quality of the DNA

The quality and concentration of DNA can be determined using gel electrophoresis wherein one can observe the presence and quality of DNA

References:

http://www.biotecharticles.com/DNA-Article/DNA-Extraction-Procedure-and-Importance-in-Forensics-389.html

http://serc.carleton.edu/microbelife/research_methods/genomics/dnaext.html

[viralphd]

Bacteria cell lysis . In this video I resuspended the bacteria pellet and added lysis buffer which turns blue as the cells are lysed. Then added precipitation buffer and this made the blue colour disappear and the solution become cloudy as proteins precipitate. Toward the end of the video you can see the cell debris moving to the top of the supernatant which has the plasmid DNA. . Next step after this is to remove the proteins and transfer the supernatant into columns for binding of the plasmid DNA . . . . . #dnaprep #dna #gigaprep #bacteria #qiagen #luriabroth #ecoli #dh5alpha #scientist #virologist #vaccine #science #bateriology #hepatitiscresearch #dnaisolation #dnaextraction #molecularbiology #genetics #phd #phdstudentlife #viralphd

[nickyhants]

Benefit from a hands-on experience with the Maxwell RSC instrument for automated DNA and RNA extraction using your own research samples if you are at Newcastle University on Wednesday 25th September 2019. Whether your group extracts from 10 or 10,000 samples, from blood, tissue, cells, FFPE, plasma etc. we would like to see you on the day. Simply message us and we will send you a link to a poll, which you will need to complete about the sample you wish to extract from (the poll takes just a couple of minutes to complete), so we know which chemistry to run (we plan to extract from 2 sample types on the day, so it is important to let us know your preference). Plus, for every sample extracted £10 will be donated to a charity of the IGM's choice. We will contact you to confirm if you would like to participate and give you a time slot to bring your samples nearer the date. If you are not at Newcastle University but would like a demonstration of the Maxwell please contact us and we will be in touch to arrange an opportunity for you to see the Maxwell in action too. . . . #LabChat #LabLife #Science #Scientists #Biology #Biologists #researchers #researchlife #LabWares #Promega #PromegaUK #MaxwellRSC #NewcastleUniversity #NewcastleUni #IGM #genomics #tissueresearch #bloodresearch #FFPE #plasmaresearch #DNA #RNA #nucleicacid #cellresearch #ilovescience #instascience #scienceisawesome #dnaextraction #rnaextraction https://ift.tt/2ZQjfTx