#FlowCytometry

[keepthatdogalive]

Witte bloedbeeld - uiterlijk van witte bloedcellen

Op granula

Basofiele granulocyten -> paarse granula

Eosinofiele granulocyten -> oranje granula - in de vorm van de granula zit wel diersoortvariatie: hond bolletjes, kat staafjes, en paard druiventrosjes

Neutrofiele granulocyten -> paarse en oranje granula, maar lastig te zien

Monocyt / macrofaag -> geen granula, maar wel vacuolen

Lymfocyt -> geen granula

Op kernvorm

Staafkernige granulocyt -> staafkernig - een onvolwassen granulocyt uit de opslag en rijpingspool die in de bloedbaan voorkomt (linksverschuiving = de verhoogde afgifte uit de opslag/rijpingspool)

Basofiele granulocyten -> segmentkernig

Eosinofiele granulocyten -> segmentkernig

Neutrofiele granulocyt -> segmentkernig

Polymorfkernige neutrofiele granulocyt -> hypersegmenteerde kern

Monocyt / macrofaag -> ronde kern - technisch gezien een ronde kern, maar in veel gevallen is dit niet een mooi rondje, wel duidelijk één blob, tegenover de duidelijker gesegmenteerde kern van de granulocyten

Lymfocyt -> erg grote ronde kern

Extra's

Normaal in de bloedbaan - staafkernige neutrofiele granulocyten - segmentkernige neutrofiele granulocyten - basofiele granulocyten - eosinofiele granulocyten - lymfocyten - monocyten (macrofagen zijn waar monocyten in veranderen als ze de bloedbaan verlaten, maar monocyten zelf hebben ook al een fagocyterende functie in de bloedbaan)

Monocytose Verhoogd aantal monocyten Bij landbouwhuisdieren: chronisch proces, want monocyten zijn acuut minder relevant bij deze dieren Bij gezelschapsdieren: acuut proces

Onderscheid maken tussen de leukocyten Flowcytometry/laser cell counter vs. microcapillair methode vs. coulter counter methode

Microcapillair methode kan geen onderscheid maken tussen de verschillende rondkernige en de verschillende granulocyten. Coulter counter methode kan geen onderscheid maken tussen de verschillende granulocyten. Flowcytometry kan geen onderscheid maken tussen staafkernige en segmentkernige neutrofiele granulocyten. Bij handmatige tellen kan er wel onderscheid gemaakt worden tussen staafkernige en segmentkernige neutrofiele granulocyten.

Stamboom (dit moet ooit verplaats worden denk ik)

Rode bloedbeeld -> klik op de link

[basavatarakam-cancer-hospital]

We are pleased to announce the success of the IAPM Mid-Term CME on Basic Concepts in Flow Cytometry with an Approach to Leukemia and Lymphoma (Blood Cancers) Diagnosis, held at Basavatarakam Indo-American Cancer Hospital & Research Institute, Hyderabad, on February 3, 2024.

Diverse Participation: Over 270 delegates attended from Telangana and Andhra Pradesh.

Tailored Scientific Agenda: Designed to meet the needs of postgraduate students and practicing pathologists, ensuring a comprehensive learning experience.

Engaging Discussions: The program was warmly received, fostering lively discussions and active participation from faculty and delegates.

National Insight: Renowned faculty from across the country presented rare and challenging cases, addressing the complexities of uncommon disease diagnosis.

Cutting-Edge Technology: Our institute proudly houses a 10-color flow cytometer, conducting over 700 case analyses annually. This includes essential work on leukemia/lymphoma diagnosis and measurable residual leukemia monitoring in acute leukemia cases.

Gratitude: A heartfelt thank you to all participants, faculty, and contributors for making this CME a resounding success.

Stay tuned for more updates on our continuous commitment to advancing medical education and expertise!

[phenomenoresearch]

🔬 Unlocking the Power of Tumor Treating Fields (TTFields) in Lung SCC 🧬

This study reveals how TTFields (100–200 kHz) impact lung squamous cell carcinoma in vitro. 🧪 Using Western Blot, Flow Cytometry, and Transwell assays, 150 kHz emerged as the most effective frequency—significantly reducing α-tubulin expression, inducing apoptosis, and inhibiting invasion. 📉🔋 These findings highlight the promising therapeutic potential of TTFields, offering a non-invasive, frequency-specific strategy for targeted lung cancer treatment. 🚀🫁 Ideal for researchers & biomedical technicians exploring advanced cancer therapies. 📊👨‍🔬👩‍🔬

#tumores #lungcancer #squamouscellcarcinoma #cancertherapy #biomedical #apoptosis #western #flowcytometry #transformation #targetedtherapy #oncology #invitro #cancerawareness #medicalresearch

International Phenomenological Research Awards

Website Link: https://phenomenologicalresearch.com/

Nomination Link: https://phenomenologicalresearch.com/award-nomination/?ecategory=Awards&rcategory=Awardee

Contact Us For Enquiry: contact@phenomenologicalresearch.com

#phenomenology #researchawards #internationalawards #academicrecognition #qualitativeresearch #phenomenologicalstudies #scholarlyachievement #researchexcellence #humanscienceresearch #professor #academic #sciencefather #voicetherapy #mentaleffort #patientperception #vocalrehabilitation #speechtherapy #cognitiveload #patientexperience #therapeuticoutcomes #voicehealth #healthcarepsychology

Youtube:https://www.youtube.com/channel/UCga5JVq6VElDLksyLzNqybg

Twitter: https://x.com/compose/post

Instagram: https://www.instagram.com/phenomenoresearch/?hl=en

Facebook: https://m.facebook.com/phenomeno002/

Pinterest: https://in.pinterest.com/phenomenoresearch/

Blogger: https://phenomenological21.blogspot.com/

Whatsapp Channel: https://whatsapp.com/channel/0029Vazl6GD7z4kkTlmIbc29

[alishaaishu2000]

Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or electromagnetic radiation, typically visible light or UV. This phenomenon occurs when electrons in a molecule absorb energy, become excited to a higher energy state, and then release energy as they return to the ground state. Fluorescence is widely applied in scientific research, imaging, and diagnostics, including fluorescence microscopy, flow cytometry, and fluorescent labeling in biomolecular studies.

International Chemistry Scientist Awards

Website: chemistryscientists.org

Contact us: contact@chemistryscientist.org

Nominate  now: https://chemistryscientists.org/award-nomination/?ecategory=Awards&rcategory=Awardee

 #sciencefather#researchawards#Professor,#Lecturer,#Scientist,#Scholar,#Researcher #Fluorescence #PhotonEmission #MolecularImaging #FluorescentLabeling #UVLight #Spectroscopy #ScientificResearch #BiomolecularAnalysis #FluorescenceMicroscopy #FlowCytometry #LightScience #QuantumMechanics #Bioimaging #PhysicsInBiology

👉 Don’t forget to like, share, and subscribe for more exciting content!

Get Connected Here: =============

Blogger : https://www.blogger.com/blog/post/edit/6961521080043227535/467226973388921229

Twitter : https://x.com/chemistryS79687

Pinterest : https://in.pinterest.com/chemistryaward/

Instagram: https://www.instagram.com/alishaaishu01/

Youtube : https://www.youtube.com/channel/UCAD_pDvz3ZHqv_3hf-N0taQ

[jobrxiv]

Research logistician/Engineer position (with PhD) in flow cytometry UNamur (Université de Namur) #JOB #ALERT: #UNamur seeks to hire an #engineer for its #flowcytometry facility! If you have a #PhD are expert in #FACS, this job is for you :-) See the full job description on jobRxiv: https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logistician-engineer-position-with-phd-in-flow-cytometry/?feed_id=73174 #cell_biology #crispr #facs #flow_cytometry #gene_editing #ScienceJobs #hiring #research

[globalinsightblog]

🔬💫 Flow Cytometry: Illuminating the Path to Precision Medicine! 💫🔬

Step into the future of medical diagnostics with flow cytometry, where cutting-edge technology meets personalized healthcare to revolutionize how we understand and treat diseases! 🌟💉

🔍 Unlocking the Mysteries of the Cell

In the intricate world of biology, every cell has a story to tell. Flow cytometry allows us to listen, decode, and analyze these stories with unparalleled precision. From detecting cancer markers to monitoring immune responses, its applications are as vast as they are transformative. 💡🧬

🚀 The Dawn of Personalized Medicine

Gone are the days of one-size-fits-all treatments. With flow cytometry, healthcare providers can tailor therapies to individual patients, optimizing outcomes and minimizing side effects. It's a paradigm shift that's putting the power of precision medicine into the hands of doctors and patients alike. 💊🎯

🌈 Shaping the Future of Healthcare

As technology continues to evolve, so too does the potential of flow cytometry. From advancements in multi-parametric analysis to the integration of artificial intelligence, the possibilities are limitless. Together, we're paving the way towards a healthier, more informed tomorrow. 🌐💪

💬 Join the Conversation

How do you envision the future of flow cytometry shaping healthcare? Share your thoughts, experiences, and dreams for a world where every patient receives the care they deserve. Let's spark a dialogue and ignite change together! 💬🌟

#FlowCytometry #PrecisionMedicine #HealthTech #Innovation #FutureOfHealthcare 🚀🔬

[elainaledoyen]

Cytokines​ are a broad and loose category of small ​proteins​ ​(5-20 kDa molecular weight) ​important in ​cell signaling​, particularly in the regulation of immune system responses to infection and inflammation. Cytokines​ are ​peptides​ and cannot cross the ​lipid bilayer​ of cells to enter the ​cytoplasm​. They communicate by signaling through cell surface receptors. Cytokines have been shown to be involved in autocrine, paracrine, and endocrine signaling pathways. ​Although secreted, cytokines are not considered to be hormones. ​There are several families of cytokines expressed in humans and other vertebrates and can act powerfully as individual molecular reagents although most often exert their influence combinatorially in multiple interactions c depending on the context in which they are expressed. ​The different types of cytokines, e.g., interferons, interleukins, lymphokines, chemokines, and tumor necrosis factors can act alone, work together, or work antagonistically in modulating a combinatorial response. Cytokines exert their influence on cells via cognate cell-surface receptors in either a paracrine fashion, i.e., by binding to receptors on other nearby cells, or in an autocrine mode, i.e., binding to receptors on the cell that secreted them.

[leucococitas]

Expresión de CD45 en Sangre Periférica ~#haematology #flowcytometry https://www.instagram.com/p/CmNh-EjNerk/?igshid=NGJjMDIxMWI=

[bkoutlooks]

#california #fremont #jobs #directhire #engineeringjobs #engineer #reliabilityengineer #flowcytometry Fremont, CA Reliability Engineer, Flow Cytometry Bachelor’s degree, engineering + 10yrs experience in reliability engineering in the medical instrument and/or medical device industry Direct Hire Negotiable Salary Job Description: ✨ - Build and lead the Reliability Engineering program to ensure products meet customer requirements. - Develop product reliability requirements based on industry standard and customer requirements - Develop reliability requirements from systems level all the way down to component level - Develop product reliability test methodologies - Lead and supervise reliability test team including engineers and technicians - Compile, analyze and interpret reliability test data - Write and review reliability reports Job Qualifications: ✨ - 10 plus years’ experience in reliability engineering in the medical instrument and/or medical device industry - Deep understanding of reliability theory and general practice - Hands on experience in developing reliability models - Hands on experience in planning and conducting reliability tests - Management experience is a plus Submit resume via: www.bkoutlooks.com (at Fremont, California) https://www.instagram.com/p/CdOtKjoujkZ/?igshid=NGJjMDIxMWI=

[doctorfoxtor]

mrrrrrp. prrrrrr. ...mrraouw?

100 days of productivity

Day 3

Close contacts: people a patient spends >8 hrs/day with

HFrEF: 1st line: ACE/ARB/beta-blockers; 2nd line: spirono/eplerenone; 3rd line: sacubitril-valsartan/ivabradine

Haemosiderin in urine + anaemia + thrombocytopaenia = chronic intravascular haemolysis; consider flowcytometry (?PNH; virtually confirmed if underlying prothrombotic state)

History strongly suggestive of MI but ST-elevations are not sufficient in amplitude = administer aspirin, heparin and pain control and check ECG again in 15 min

Vertebral metastasis w/o cord compression = chemotherapy; w/ cord compression = radiotherapy

Lamotrigine can worsen myoclonus

Fabry disease FABRYCaL: Foam cells/Fever, Angiokeratomas/Anhidrosis/Alpha-galactosidase A deficiency, Burning neuropathy, Renal disease with maltese cross lipid sediment, Conduction defects/Corneal clouding/Ceramide trihexoside accumulation, Lymphoedema

Inferior optic chiasm compression = superior visual field impairment → macroadenoma; superior optic chiasm compression = inferior visual field impairment → think craniopharyngioma

Codeine to morphine conversion → divide by 10

[jobrxiv]

Research logistician/Engineer position (with PhD) in flow cytometry UNamur (Université de Namur) #JOB #ALERT: #UNamur seeks to hire an #engineer for its #flowcytometry facility! If you have a #PhD are expert in #FACS, this job is for you :-) See the full job description on jobRxiv: https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logistician-engineer-position-with-phd-in-flow-cytometry/?feed_id=72537 #cell_biology #crispr #facs #flow_cytometry #gene_editing #ScienceJobs #hiring #research

[viralphd]

My beautiful cell pellets🔬🤓 . . . . . . . . #intracellularcytokinestaining #ics #immunologylab #spinbabyspin #centrifuge #medicalresearch #hepatitiscvaccine #hepc #immuneresponse #cd4cd8 #tcells #flowcytometry #molecularbiology #immunology #cellpellets #96wellplate #science #laboratory #phdscientist #science #antibodies #fluorophore #experiment #molecularbiology #immunology #scientist #phd #phdstudent #lablife #phdlife #viralphd

[elainaledoyen]

Innate immunity is the initial immune response to infectious agents, carried out mainly by​ granulocytes and macrophages. These include a variety of responses that are genetically hard-wired to mount a quick response to families of molecules common to classes of pathogens. Adaptive (or acquired) immunity creates immunological memor​y after an initial response to a specific pathogen, leading to an enhanced response to subsequent encounters with that same pathogen. This process of acquired immunity is the basis of vaccinatio​n.​

[leucococitas]

Más detalles en nuestro blog https://leucococitas.blogspot.com/ #CD4 (martes de cluster of differentiation) #hematologia #flowcytometry #CD4 (en Chile) https://www.instagram.com/p/Cf5SBKauSwj/?igshid=NGJjMDIxMWI=

[rnomics]

IJMS, Vol. 23, Pages 14277: Tumor Cell Capture Using Platelet-Based and Platelet-Mimicking Modified Human Serum Albumin Submicron Particles

The co-localization of platelets and tumor cells in hematogenous metastases has long been recognized. Interactions between platelets and circulating tumor cells (CTCs) contribute to tumor cell survival and migration via the vasculature into other tissues. Taking advantage of the interactions between platelets and tumor cells, two schemes, direct and indirect, were proposed to target the modified human serum albumin submicron particles (HSA-MPs) towards tumor cells. HSA-MPs were constructed by the Co-precipitation–Crosslinking–Dissolution (CCD) method. The anti-CD41 antibody or CD62P protein was linked to the HSA-MPs separately via 1-ethyl-3-(-3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) EDC/NHS chemistry. The size of modified HSA-MPs was measured at approximately 1 µm, and the zeta potential was around −24 mV. Anti-CD41-HSA-MPs adhered to platelets as shown by flowcytometry and confocal laser scanning microscopy. In vitro, we confirmed the adhesion of platelets to tumor lung carcinoma cells A549 under shearing conditions. Higher cellular uptake of anti-CD41-HSA-MPs in A549 cells was found in the presence of activated platelets, suggesting that activated platelets can mediate the uptake of these particles. #RNA-seq data in the #cancer Cell Lineage Encyclopedia (CCLE) and The #cancer Genome Atlas (TCGA) database showed the expression of CD62P ligands in different types of #cancers. Compared to the non-targeted system, CD62P-HSA-MPs were found to have higher cellular uptake in A549 cells. Our results suggest that the platelet-based and platelet-mimicking modified HSA-MPs could be promising options for tracking metastatic #cancer. https://www.mdpi.com/1422-0067/23/22/14277?utm_source=dlvr.it&utm_medium=tumblr

[bkoutlooks]

#tennessee #brentwood #brentwoodTN #medicaltechnologist #medtech #flowcytometry #jobs #directhire Brentwood, TN Validation Specialist II /Medical Technologist III Direct Hire Negotiable salary [Bachelors in Life Science or related; 2-3yrs flow cytometry or related laboratory experience; flow cytometry analysis experience a plus - WinList/FlowJo/FACSDiva, etc.] https://www.instagram.com/p/B_LDsrXHlE8/?igshid=sb7q8d4wg9ft