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#NGS library prep
tekmaticinc · 1 year
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Linear Electric Positioners: Empowering Precision Motion Control for High-Tech Industries
Linear Electric Positioners (LEPs) are the solution to achieving the highest level of precision in motion control. At TekMatic, we have been providing high-tech engineered solutions for over 30 years, and our LEPs are among the most advanced and reliable on the market.
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litttlestars · 4 months
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hi!! this is a bit late but for the book asks, could i ask: 6 - was there anything you meant to read, but never got to?, 14- what books do you want to finish before the year is over? and 19 - did you use your library? :)!!
6. oh my gosh SOOOO many books. literally so so many but life and mental health has a funny way of getting in the way of passions and hobbies :') some notable ones are:
the house on the cerulean sea
crying in hmart by michelle zauner
the atlas six by olivie blake
tom lake by ann patchett
14. unfortunately im answering this WAYY too late and the year is already over :(( BUT BUT BUT here are some books i really really want to read this year
finish a little life
finish the invisible life of addie larue
a good girl's guide to murder
happy place by emily henry
little fires everywhere by celeste ng
19. i did notttt in terms of book borrowing :( but i did go to my local library a bunch of different times to study!!! yeah ermmm prepping for med school has been super rough
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delvenservices · 2 months
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Next Generation Sequencing (NGS) Market Outlook, Trends, Size, and Share Analysis
Next Generation Sequencing (NGS) Market analysis report figures out market landscape, brand awareness, latest trends, possible future issues, industry trends and customer behaviour so that the business can stand high in the crowd. It includes extensive research on the current conditions of the industry, potential of the market in the present and the future prospects from various angles. This market report comprises of data that can be pretty essential when it comes to dominating the market or making a mark in the Healthcare industry as a new emergent. To bestow clients with the best results, Next Generation Sequencing (NGS) Market research report is produced by using integrated approaches and latest technology.
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Next Generation Sequencing (NGS) Market Competitive Landscape:
10X Genomics
Agilent Technologies
BGI Group
Bio-Rad Laboratories Inc.
Brooks Automation Inc.
CD Genomics
DNASTAR Inc.
Eurofins Scientific
F. Hoffmann-La Roche Ltd.
Illumina Inc.
Macrogen Inc.
New England Biolabs
Novogene Corporation Inc.
Oxford Nanopore Technologies
Pacific Biosciences of California Inc.
PerkinElmer Inc.
Precigen Inc., (Intrexon Bioinformatics Germany GmbH)
Promega Corporation
Psomagen
Qiagen
Takara Bio
Tecan Trading Group
Thermo Fisher Scientific Inc.
Zymo Research
Next Generation Sequencing (NGS) Market Recent Developments:
In January, 2023, QIAGEN announced a strategic partnership with California-based population genomics leader Helix to advance next-generation sequencing companion diagnostics in hereditary diseases.
In March, 2023, The company SOPHiA GENETICS announced a new partnership with Qiagen that will pair QIAseq reagent technology with the DDM platform to enhance tumor analysis through next-generation sequencing (NGS).
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The global Next Generation Sequencing (NGS) market size was estimated at USD 11.59 billion in 2023 and is projected to reach USD 29.84 billion in 2030 at a CAGR of 14.47% during the forecast period 2023-2030.
Next-generation sequencing (NGS) is a massively parallel sequencing method that offers scalability, high throughput, and high speed for genome-wide sequencing of nucleotides. Because it involves the preparation of material for the subsequent sequencing reaction, DNA presequencing is one of the most important processes in a common sequencing protocol. Globally, NGS is increasingly integrated into clinical laboratory analysis, testing and disease diagnosis in the healthcare industry. In pharmacogenomics, next-generation sequencing (NGS) is often used to accelerate the drug discovery process.
The growth of the global next-generation sequencing market is primarily driven by the growth of genome mapping programs; proliferation of next-generation sequencing applications; rising health care costs; and technological advances in sequencing platforms. For example, in 2019, Thermo Fisher Scientific, a world leader in scientific services, launched a new generation platform, the Ion Torrent Genexus System, which provides sample for one-day reporting. Additionally, Agilent Technologies, Inc., an American analytical development and manufacturing company, brought in 2019, we launched the Magnis NGS Prep System, a next-generation sequencing library preparation system designed to perform complex sequencing analyzes at the push of a button. Fetal genetic makeup and configuration can be detected with NGS-oriented tests such as non-invasive prenatal testing (NIPT) and pre-implantation genetic testing (PGT). R&D-related NGS has risen to new heights thanks to investments in NGS technologies worldwide. Growing demand for genome mapping software and increasing use of NGS is fueling market growth.
Scope of the Next Generation Sequencing (NGS) Market Report:
The Next Generation Sequencing (NGS) Market is segmented into various segments such as product, application, technology, end-user and region:
Based on Product
Consumables  
Sample Preparation Consumables  
DNA Fragmentation, End Repair, A-Tailing and Size Selection
Library Preparation & Target Enrichment
Quality Control
Other Consumables  
Platforms
HiSeq
MiSeq
Ion Torrent
Solid
Pacbio Rs II and Sequel System
Other Sequencing Platforms
Services  
Sequencing Services  
RNA Sequencing
Whole Exome Sequencing
Whole Genome Sequencing
Targeted Sequencing
Chip Sequencing
De Novo Sequencing
Methyl Sequencing
Data Management Services  
NGS Data Analysis Services
NGS Data Analysis Software & Workbenches
NGS Storage, Management and Cloud Computing Solutions
Based on Application
Diagnostics
Biomarkers and Cancer
Reproductive Health
Personalized Medicine
Agriculture and Animal Research
Other Applications
Based on the Technology
Sequencing by Synthesis
Ion Semiconductor Sequencing
Sequencing by Ligation
Pyrosequencing
Single Molecule Real Time Sequencing
Other Technologies
Based on End User
Academic and Research Centers
Pharmaceutical and Biotechnology Companies
Hospitals and Clinics
Other End Users
Based on Region
Asia Pacific  
North America  
Europe
South America  
Middle East & Africa  
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• Which global Next Generation Sequencing (NGS) Market trends, challenges, and important competitions of the economy have faced?
• Which are the major final effect and outcomes of the analysis of the advantages of the industry?
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Point of Care Testing Market
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Vascular Imaging Market
Magnetic Resonance Imaging (MRI) Coils Market
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repromed15062023 · 1 year
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Dna Fragment And Library Prep Equipment, Sparq Vwr
However, as with most fertility issues, Jessica urges couples not to panic should you get a foul outcome. "Experts are pointing to the actual fact now that the sperm analysis is unreliable and pretty useless. And but when a pair goes for their preliminary round of exams the woman is told to strive clomid to come out a couple of extra eggs" she continues. At present, 32,000 women are handled for infertility in the UK per year, whereas there is an 8-9pc improve every year throughout Europe in the variety of couples presenting to clinics.
Nevertheless, in humans, a fraction of spermatozoa has a relative excessive diploma of DNA fragmentation. This injury can derive from aberrant chromatin packaging during spermatogenesis, defective apoptosis, or excessive manufacturing of reactive oxygen species within semen analysis the ejaculate. Furthermore, DNA harm can be induced by environmental exposure to genotoxic brokers whose effectivity relies upon also on the accessibility of DNA (Bennetts & Aitken 2005, Chohan et al. 2006, Perry 2008, Sakkas & Alvarez 2010).
This may cause hormonal imbalances in men leading to lowered sperm amount and likewise affecting high quality and DNA fragmentation. Aim to reduce your plastic exposure, avoid handling until receipts, use extra pure private hygiene merchandise and try to eat as natural produce the place sperm count test you can. We know that conception charges improve through the summer time which may show a job for vitamin D in fertility and sperm have receptors for vitamin D. Be cautious when supplementing with vitamin D; it is essential to tailor your complement ranges to meet your wants.
As shown by the histograms, in one other way from DNAse I treatment, the quantity of DNA lesions induced by H2O2 was related within the completely different species. The alkaline comet assay gave a dose-dependent enhance in the imply values of fraction tail DNA, whereas this parameter was not enhanced utilizing the neutral protocol, reinforcing the notion that H2O2 prevalently induces DNA SSBs. The TUNEL assay (Terminal Transferase dUTP Nick End Labelling), the sperm chromatin construction assay (SCSA) and the COMET assay (the single-cell gel electrophoresis assay). Each test measures completely different elements of sperm chromatin damage and consequently have variable ranges of diagnostic and prognostic energy. A semen analysis is amongst the most necessary exams within the evaluation of Male fertility.
Now, she has six staff and all the testing on the samples to-date is done in their workplaces and labs on the Royal Victoria Hospital web site. The health of a man’s sperm not solely impacts his fertility but it can have an result on the well being of his child, too. Diseases similar to epilepsy, autism and cancer have all been linked to sperm from older men the place the chance of sperm DNA harm is far larger. One in six couples will fail to conceive and, in 40pc of instances, the primary drawback is on the man’s aspect.
Furthermore, the Trk signaling cascade have to be activated for this response to occur. Because the expression of those receptors diminishes in adulthood, neurotrophin utility may dna fragmentation test be most appropriate within the pediatric inhabitants. Sequencing information from DNA fragment libraries generated from 50 pg to a hundred ng of sheared E.
Fertility issues can cause huge stress to individuals, who are normally 30-somethings at the top of their careers and maybe haven’t faced a unfavorable life occasion but. The strain on them is so great, it can male fertility test ireland even lead to relationships breaking down. Examen analysis reveals that sperm from men whose partners have a history of miscarriage have higher levels of sperm DNA fragmentation than sperm from these whose companions ship wholesome infants (Figures 7 and 8).
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singaporenewstribe · 1 year
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Telesis Bio Announces First Commercial Shipment of BioXp® NGS Library Prep Kit
http://dlvr.it/SpFGwH
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evoldir · 1 year
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Fwd: Job: ClemsonU.LabTech.GenomicsBioinformatics
Begin forwarded message: > From: [email protected] > Subject: Job: ClemsonU.LabTech.GenomicsBioinformatics > Date: 13 January 2023 at 07:00:40 GMT > To: [email protected] > > > We are looking to hire a Lab Research Specialist with a focus in > molecular biology and lab management at the Clemson University Genomics > and Bioinformatics facility. > > For more information: [email protected] > > https://bit.ly/3GJaHmm > > > JOB SUMMARY: > Laboratory Research Specialist will assist in day-to-day lab management > duties, supervise and train student and postdoctoral researchers, conduct > experiments, perform basic bioinformatic analyses, conduct other genomic > related research activities and perform other duties as assigned. > > JOB DUTIES: > 55% - Essential - Research Activities: Performs technical molecular > biology experiments, including but not limited to: DNA/RNA isolation, > cDNA prep, RNA/DNA library preparation, Illumina short-read NGS, qPCR, > PCR, etc. Performs basic bioinformatics tasks such as de-multiplexing, > trimming NGS reads, etc. Oversees and runs the Agilent Bioanylzer, > the Illumina NGS sequencing platforms and other equipment. > > 30% - Essential - Lab Management:  Manages lab ordering, invoicing, > budgeting, record keeping and generation of quotes. Assists > in maintaining regulatory compliance. Manages lab inventory and > records. Maintains/Updates social media/website and helps organize > seminars/workshops. Oversees day-to-day maintenance of lab space, > facilities, and equipment. > > 15% - Essential - Personnel Oversight:  Assists in training personnel, > students, staff, and faculty levels.  Assists in providing oversight > for student researchers. > > MINIMUM REQUIREMENTS: > Education -  Bachelor's Degree - Biology or related field > Work Experience 2+ years > > > > > > Christopher L. Parkinson > Department of Biological Sciences, and > Department of Forestry and Environmental Conservation > Director, Clemson University Genomics and Bioinformatics Facility > 190 Collings St., Life Sciences Facility, Clemson University, Clemson, > SC 29634 > > Christopher L Parkinson
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isucheta · 2 years
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DNA Digital Data Storage Market Overview 2020 to 2028, Future Trends and Forecast
Twist Bioscience and Microsoft Corporation - Notable Market Players in DNA Digital Data Storage Market
The DNA digital data storage is the method of encoding and decoding binary data to and from synthesized strands of DNA. The DNA has garnered interest as a medium for digital information storage due to its high storage density and durability that is superior to those of existing silicon-based storage media. Moreover, DNA’s four-letter nucleotide code provides a suitable coding environment that can be used in the same way that computers and other electrical devices employ binary digital code to represent any letter, digit, or other character.
The DNA digital data storage market is majorly comprised of top players involving, Illumina, Twist Bioscience, Agilent Technologies, Inc., Molecular Assemblies, Quantum Corporation, CATALOG, Microsoft Corporation, Iridia, Inc., F. HOFFMANN-LA ROCHE LTD., DNA Script and Evonetix among others.
 The companies listed above are implementing various strategies such as product launches, acquisitions, mergers, and partnerships, which have resulted in the company's growth and, in turn, have brought about various changes in the worldwide market. Additionally, the companies have adopted several inorganic and organic strategies for accelerating their growth and improving their market position.
Below is the list of the growth strategies done by the players operating in the DNA digital data storage market:
Year
News
Dec-2021
Twist Bioscience Corporation launched the Twist 96-Plex Library Prep Kit, a high-performance, cost-effective preparation kit for next-generation sequencing (NGS). Recently, new methods in genotyping by sequencing (GBS) have unlocked the potential for NGS to improve data quality and quantity, deliver significant improvements in lab workflows and reduce sample prep costs.
Feb-20222
Twist Bioscience Corporation and Abcam announced a licensing agreement under which Abcam will use a proprietary Twist VHH phage library for antibody discovery, development and commercialization for research and diagnostic applications.
Jan-2022
Twist Bioscience Corporation and Artisan Development Labs Inc. announced a collaboration  to discover novel antibodies against five undisclosed targets, with an option to expand with additional targets.
Dec-2021
CVS Health and Microsoft Corp announced a strategic alliance to develop innovative solutions to help consumers improve their health.
Nov-2021
Kyndryl and Microsoft Corp announced a partnership that will combine their market-leading capabilities in service of enterprise customers.
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vijayanger12 · 2 years
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The NGS sample preparation market is expected to cross the revenue of US$ 4.1 Bn by 2030, as automation is gauging strong business grounds in reagent handling, right from isolation to library prep.
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tekmaticinc · 1 year
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What is NGS Library Preparation? How Does it Work?
The resulting libraries can be used for various applications, including genome sequencing, gene expression analysis, and DNA methylation analysis. This blog will discuss the multiple steps involved in NGS library prep and the importance of this process in modern genomics research.
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sunb0rn · 2 years
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bagong fave sa lab
part ng library prep for DNA sequencing
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gascon-en-exil · 3 years
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Three Houses 100% Completion: Refining Those Spreadsheets
To my utter surprise, @symsykins has expressed interest in trying out my long and convoluted idea for a 100% NG+ run of FE16. Far be it from me to stifle the ambitions of a fellow completionist, but as I've made a fair number of updates to the methodology of that post in the months since I wrote it I would be remiss if I didn't publish those changes for the benefit of him or anyone else who wants to try their hand at this absurdity. Sometimes playtesting this stuff can be more entertaining than actually carrying it out....
Additional Parameters
It occurred to me that for "true" 100% completion you also need to complete those things tied to your Switch account rather than individual files. This means filling out the support log, event gallery (Goddess Tower events), and music library. Fun fact: there are exactly 29 Goddess Tower events if the same-sex ones are counted twice as the game does, so provided you plan them out ahead of time you shouldn't have to save scum to get them all.
As mentioned in the earlier post most of this is trivial to complete. The easiest way to raise support points between an active unit and a classroom unit is to have the latter serve as an adjunct during battle. They don't need to have an equipped weapon or be in a class other than noble/commoner, but just having them there will rapidly build supports. Still, it's best to plan out and stagger the number of supports your units take on each run so they're not overloaded. Cross-house supports are one of only two reasons to ever do cross-house recruiting, and they should also be staggered accordingly.
There's also the matter of building up your battalion guild. There are 127 unique battalions to obtain, discounting the Essar Research Group which requires the game's online features and a Switch Online subscription to access. Your guild can fit 200 total battalions in it, so there's ample room to acquire and max out one of each battalion as well as duplicates for the better ones. This is also the only other reason to recruit across houses: to get the three Part 2 paralogues that require it (Ferdinand/Lysithea, Linhardt/Leonie, and Caspar/Mercedes) and so unlock their associated battalions. As with recruiting for cross-house supports, you should only bother doing these once.
One-Time Renown Purchases
You can and should buy all the Renown purchases in Abyss and most of the saint statue bonuses during your first run, using the 10000 Renown bonus from CS and all incidental Renown gained during the run to do so as you can't spend it on anything else. Note that the last bonus for each statue, a boost to units' max stats, is functionally useless for the purposes of this project and so can be left for the second run - a full 10000 Renown you won't have to worry about spending right away. At any rate, however, you'll have amassed enough Renown to complete the saint statues during your second run.
On Monastery Activities
Previously I'd discounted the greenhouse as an unnecessary time sink like fishing and tea time, only to be completed when a quest is involved. However, I find that in non-CF runs you'll quickly start running out of dining hall resources in Part 2 without planting crops, which forces you into the less efficient alternative of running around and throwing gifts at your active units to max out their motivation each week. Therefore I recommend planting vegetables and fruit in the greenhouse throughout Part 1, at which point you can stop because by then you should have accumulated enough resources to cover the rest of the game. Additionally, there are certain glowing blue spots along a typical exploration run route that are guaranteed to drop fish or edible crops - at the stall in front of the fishing pond and on the stalls in the corner of the marketplace where the special merchants gather, namely - which you should check out each month.
Byleth's Level
When Byleth is not an active unit don't bother raising their level higher than 15. This is good enough to recruit all the level-dependent characters (most of the church units), and the only meaningful benefit to going up to 20 is to attempt the proper conduct tournament. However, early on Byleth is unlikely to be able to win the tournament even at 20 without an appropriate class or abilities, and the Renown and brave weapon frankly are not worth the waste of time and space in having them be an adjunct for those five extra levels. Save the tournament for runs where you're actually using Byleth.
Classroom Unit Ranks
A quick correction here. I previously said that Authority should be every character's first priority as a classroom unit, but I no longer believe this is true. With standardized class sets (see further down) it's more important that characters look ahead to the runs they'll be doing in the near future and work on skills relevant to those runs. This especially holds true for banes; a classroom unit can easily take a bane skill as high as B or even A from base over the course of a run, which will help significantly with future certification requirements. Classroom units can work on Authority, but I'd save that for those that have free availability in most or all runs like Anna and the Wolves. The regular students and to a lesser extent non-student characters that join late will want to prioritize their problem skills and build Authority mostly during combat in their active runs.
Route Distribution
I had previously suggested that CF should have the most runs to compensate for Jeritza being awful...but screw that. My current 29 run setup:
6 runs of SS
10 runs of AM
9 runs of VW
4 runs of CF
Notably, this works out to 9 or 10 runs per house which is overall more significant given the advantages in-house units enjoy. The choice to give AM an extra run over VW came down to AM having more exclusive units; VW's one extra week isn't that much of an advantage, not when the Deer lack a Part 2 exclusive and also include Lysithea. But, you ask, can you get away with running CF that little?
Playtesting suggests yes, provided those runs follow some strict parameters: five units only, with three of them being the CF exclusives and the remaining two slots covered by a rotation of the other Eagles. SS runs should precede CF runs whenever possible, to give Edelgard, Hubert, and the rest of their house more classroom time to make up for having to share runs of the shortest route in the game with Jeritza. Jeritza should always hold one of the Knowledge Gems during Part 2, with Edelgard and Hubert sharing the other. The other Eagles can lean on their active use in SS runs to make up for the deficit, and even Edelgard and Hubert can prep for their active runs or finish up skill ranks in SS. Jeritza though....
Number-Crunching Jeritza
To illustrate why I complain about Jeritza as much as I do (from a unit perspective, that is - it's been well-established why I hate him as a character), let's take a particular trouble spot of his as an example. Jeritza has an Authority bane, which means that even with Mastermind and a Knowledge Gem equipped he'll gain no more than 3 points of it during each combat encounter, or 6 if he uses a gambit (not to be relied on given how limited in use per map gambits are). He doesn't have access to any classes that boost Authority, so this is the best it gets. Starting at B Authority means it'll take him 2920 total points to reach S+. Assuming no classroom instruction (because he's got ten other skills to work on, you know) and no gambit use, that works out to him needing about 974 total combat encounters or an average of 244 encounters in each of his four runs.
Is that feasible? If you follow my method of exploring on weekends before instruction weeks and battling on other weekends (and assuming your first run is not CF which...no) there are (3 battles per weekend x 5 calendar months) + 6 chapter maps = 21 battles that Jeritza will fight. Obviously those battles vary in length, but that comes to an average of 12 combat encounters per battle. That's admittedly a bit steep, especially earlier on when few of your other units will have maxed Authority and can be used as adjuncts, but remember that gambits count as two combat encounters and that, more significantly, the other units will eventually max out Authority and allow you to field only three frontline units at a time. It does help that both Edelgard and Hubert have Authority boons, so they should be backline-ready no later than their second active run.
Unfortunately there is one little hiccup to this. CF's first calendar month consists of just one free weekend and the chapter map. As annoying as it is the journal can only be accessed while exploring, which means that on each CF run after the first you'll have to choose between skipping three battles that month or updating Jeritza's skill ranks. You should always choose to battle, because it will help out your other units (who already end up screwed out of a few battles thanks to what choosing CF does to the end of Part 1) and also allow Jeritza to start working on ranks he hadn't touched in earlier runs. The wasted Authority growth is a shame, but at least Jeritza has the base ranks to certify for any beginner class right out the gate so he can pick up a sword/lance/axe/gauntlet/Thunder and still make use of the training time. CF Chapter 13 is also unusually short and will be finished in only a few turns, so it's not a great loss either.
And all that was just for one rank! Hopefully that proves my point about Jeritza sucking, even if mercifully his class masteries and other skill ranks are less of a pain. Even faith can be worked around with bishop and holy knight offering 6 points per combat encounter, although he'll probably want to save his monk run until he can be an adjunct so he's less hobbled by his tiny spell lists.
Standardized Class Sets
Probably the part of this update I'm most proud of, as I put a lot of thought into simplifying one of the more needlessly complex elements of my original setup. Crafting individualized class sets for each character may sound fun on paper, but it's a hassle to keep track of when actually playing the game and doesn't have any substantial benefit. Characters can patch up their banes through classroom training, and furthermore class certification requires nothing higher than A which is a major reason that mastering classes is more of a rote structural element of individual runs than something that requires intricate plotting.
As such, have some standardized sets. These come in male and female versions to account for genderlock and similar considerations, and they apply to all characters except Byleth and the house leaders who have to be handled a bit differently. Noble and commoner are not listed as they're trivial to master; just make sure that non-student characters go back and do so at some point, like when they're being used as adjuncts for supports.
Below each set is a list of the skills needed to guarantee certification in all of the set's classes. This was a useful metric for determining which classes went best where.
Myrmidon
Male
Mercenary
Thief
Dancer (if available)
Hero
Swordmaster
Assassin
Trickster
Death Knight (Jeritza only)
Mortal Savant
A Swords C Axes C Bows B+ Reason B Faith
Female
Mercenary
Thief
Dancer (if available)
Swordmaster
Trickster
War Cleric
Mortal Savant
Gremory
A Swords B+ Brawling A Reason A Faith
There are a lot of sword-based classes, more so than any other weapon or magic type, and that combined with the rarity of sword banes - only Bernadetta and Mercedes - means that often even characters neutral in the skill will max out swords during the course of their myrmidon run.
This run has the distinction of being the only one to not require any of the movement type ranks, which is one reason why gremory is here. The other is that female characters have eight regular master classes as opposed to males' seven, so while when divided across four runs males get a free space here (which Jeritza takes advantage of), females do not. Unfortunately mortal savant is the only master class that proceeds naturally from all the earlier sword classes, but if you'll notice from the male set's requirements there are fairly high reason and faith requirements here even without gremory.
The other oddity is war cleric, which takes the place of assassin (see below) but stands awkwardly as female characters' only serious brawling class. The secondary faith requirement slots easily into a run that also includes trickster, but as I explained in the first post brawling is going to be a problem skill for female characters no matter what you do.
One more note: it's important to consider certain things when deciding which active units will go in which run. Myrmidon runs require the most scrutiny as this is where characters will be dancers, and of course you can only have one of those per run. These runs also require the limited availability Abyssian Exam Passes, one for males and two for females, so keep that in mind as well.
Soldier
Male
Armored Knight
Cavalier
Archer
Fortress Knight
Paladin
Sniper
Great Knight
Bow Knight
A Lances B+ Axes A Bows A Heavy Armor A Riding
Female
Cavalier
Archer
Pegasus Knight
Assassin
Paladin
Sniper
Falcon Knight
Bow Knight
B Swords A Lances A Bows A Riding B+ Flying
Here we see the major influence of genderlocked classes. Female characters have more obvious lance options thanks to the pegasus classes, whereas males have to take the heavy armor classes instead. This balances out in the fighter runs, but for now it's worth pointing out that characters will only be using lances and bows in these runs. Swords (females) or axes (males) will be trained entirely in the classroom, with females running assassin as a bow class and males running the heavy armor classes with lances. Hey, great knight does have Lancefaire, and more importantly all the classes mentioned also boost those weapon types.
Fighter
Male
Brigand
Brawler
Wyvern Rider
Warrior
Grappler
War Monk
Wyvern Lord
War Master
C Lances A Axes A Brawling C+ Faith A Flying
Female
Armored Knight
Brigand
Fortress Knight
Wyvern Rider
Warrior
Wyvern Lord
Great Knight
C Lances A Axes A Heavy Armor B+ Riding A Flying
This is the inverse of soldier, with male characters taking a quartet of brawling classes while females go mono-axes with the heavy armor classes and others. The female set also has one fewer class than all the others here, a consequence of there being one fewer female-exclusive class than male. As with the master class free space in male myrmidon, keep that in mind for later. The singular axe focus and smaller number of classes compensate for female fighters needing to train all three movement types here, which is annoying but necessary. I tried numerous combinations of classes in the soldier and fighter runs, but incredibly this is the one that's least demanding on overall ranks. What can you do?
Male fighters require an Abyssian Exam Pass - just pointing that out.
Monk
Male
Mage
Dark Mage
Priest
Warlock
Dark Bishop
Bishop
Dark Knight
Holy Knight
C Lances A Reason A Faith A Riding
Female
Mage
Priest
Warlock
Bishop
Dark Flier
Valkyrie
Dark Knight
Holy Knight
C Lances A Reason A Faith A Riding C Flying
With the understanding that gremory is covered in the myrmidon run, there's little to mention here. Rank requirements are almost identical with females only needing to work on flying a bit. These runs are the most demanding on limited seals though, with males needing two Dark Seals and females two Abyssian Exam Passes. Make sure you plan accordingly.
Also, these runs make it clear that if you're going to equip these characters with a weapon for when they run out of spells (as might well happen during earlygame), make it a lance.
Edelgard
From the standard sets:
Add lord to myrmidon
Add armored lord and emperor to fighter
Because female fighters are already one class short Edelgard can add two classes there and still come out as even as possible. She's the only character other than Byleth with more than eight master classes, meaning she'll have to do three of them in one run, but with her it's no trouble aside from emperor having the worst availability of any class in the game that is. Her unique classes slot well into fighter as it is.
Dimitri and Claude
From the standard sets:
Add lord to soldier
Move hero from myrmidon to fighter
Add high lord and great lord/wyvern master and barbarossa to myrmidon
The guys, meanwhile, don't fare as well. They take advantage of the same free space for a master class that Jeritza does, but to make the rest of this happen things have to get a bit messy. They'll have to have a certain sword rank for their soldier and fighter runs now, but as they both have sword boons that's not too tall of an order. They can also run hero as an axe class and lord as a lance class to match those runs' themes, which also helps each of them a bit in a different way - Dimitri with his axe bane, Claude with his lance bane. Dimitri's unique classes fit well with myrmidon as they also boost swords, but the best you could say about Claude's unique classes in myrmidon is that he won't have to train up bows for assassin when he can just switch to using them in wyvern master.
Byleth
The Avatar’s special gender-variable self needs class sets built from the ground up to accommodate their ability to access all of the genderlocked classes. As follows:
Myrmidon (male)
Mercenary
Thief
Dark Mage
Hero
Swordmaster
Assassin
Dark Bishop
Trickster
Enlightened One
Mortal Savant
Soldier (female)
Cavalier
Archer
Pegasus Knight
Paladin
Wyvern Rider
Sniper
Falcon Knight
Wyvern Lord
Bow Knight
Fighter (male)
Armored Knight
Brigand
Brawler
Fortress Knight
Warrior
Grappler
War Monk
Great Knight
War Master
Monk (female)
Mage
Priest
Warlock
Bishop
Dark Flier
Valkyrie
Dark Knight
Holy Knight
Gremory
Apart from the two female runs having three master classes apiece - unfortunate but necessary, as with Edelgard - the biggest surprise here should be dark mage/bishop in myrmidon. They need to be in a male run though, and there are more magical female-exclusive classes so that's not going to be monk. Alongside the standard mortal savant it's not much of a stretch in terms of skills (something Byleth never struggles with anyway because faculty training is a thing), but the required two Dark Seals should be considered.
Conclusion
I think that's just about everything for now? I could be really over the top and map out exact routes for exploration, efficient paralogue choices, and more nitpicky stuff, but even I've got my limit. Part of the fun of 100% is being able to adapt your strategies anyway, so I'm not sure that being that precise would be the best way to approach hypothetically helping someone else do something like this.
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medispec · 3 years
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Automated Liquid Handling
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Medispec (I) LTD
A Wing / 601, Lotus Corporate Park, Jay Coach, Off Western Express Highway, Goregaon (East), Mumbai - 400 063, India. +91-7718810036 / 7718810037 +91-7718810038
www.medispec.in [email protected]
Agilent's space-saving and flexible automated liquid handling solutions enable simplified sample preparation with consistent high accuracy. Automation allows labs to free up manual labor and run more samples while maintaining reproducibility. Application-specific lab automation liquid handling solutions address sample preparation needs for proteins, metabolites, as well as genomics and NGS applications, reducing set up times and protocol development. Our powerful open liquid handling platform can be tailored to serve a broad range of application needs, with more than 60 deck accessories spanning from magnetic bead separation and vacuum filtration to heating/cooling and shaking. Products for plate sealing, barcode labeling, and centrifugation bring additional capabilities to your lab. Intuitive instrument control software allows easy startup and requires little training effort, for further increased lab productivity. For Research Use Only. Not for use in diagnostic procedures.
Turnkey automated sample preparation solutions simplify your routine protocols
Manual sample preparation is an overwhelming and time-consuming task that creates bottle necks in your routine lab. To free up time and achieve consistent upstream sample prep, Agilent has developed dedicated automated liquid handling applications. The advanced cartridge technology in the AssayMAP Bravo Protein Sample Prep Platform enhances consistency and versatility of your protein assays, while the Bravo Metabolomics Sample Prep Platform is equipped with on-deck accessories and software specifically designed to automate metabolomics sample preparation procedures. Routine genomics applications and next-generation sequencing (NGS) workflows can be automated without impacting data quality using pre-developed methods for the most common library prep kits on the Bravo NGS. Throughput can be dramatically increased with the NGS Workstation. For Research Use Only. Not for use in diagnostic procedures.
AssayMAP Bravo Protein Sample Prep Platform
The AssayMAP Bravo platform automates protein sample preparation upfront of LC/MS analysis aimed at protein characterization and quantitation. Ideal for routine biopharma and proteomics labs, automated immunoaffinity capture, digestion and desalting steps, the system can run 8 to 96 samples in parallel for each sample prep application. With the AssayMAP Bravo you achieve better reproducibility and higher recovery rates of your protein sample, with our micro-chromatography column tips providing state-of-the-art binding consistency. Less hands-on time using verified protocols at significantly lower consumable cost will increase your lab’s productivity instantly. AssayMAP Bravo helps to extract the most data points possible from your precious samples, achieving the highest protein and peptide identification matches.
Add automation to your lab for consistent and accurate sample preparation
Agilent’s automated liquid handling platforms are flexible and compact automation solutions that streamline your routine laboratory microplate workflows. The BenchCel WorkStation simplifies plate sealing and labeling, while the Bravo BenchCel Workstation provides plate stamping and serial dilutions. The Bravo Automated Liquid Handling Platform has been designed to support a smooth transition from manual to automated sample preparation. This automated liquid handling robot offers various deck accessories to accommodate quick automation of your application protocols. VWorks software adds to the seamless transition with its easy-to-use interface. For Research Use Only. Not for use in diagnostic procedures. Bravo Automated Liquid Handling Platform The Agilent Bravo is a flexible liquid handling platform that automates your sample preparation for screening applications such as compound management, cell-based assays, and biochemical assays. With this robotic liquid handling system, time is freed up and consistent data are achieved across samples and users. The compact liquid handler can be easily placed on a small bench and fits in a flow hood. With more than 60 accessories ranging from heater/cooler to shaker, the system can be tailored to automate most sample preparation protocols. Automating your sample prep protocol has never been easier with the included VWorks control software that allows users to create and run flexible liquid handling protocols quickly.
Streamline your common microplate workflows
Agilent automated microplate management instruments and robotics systems revolve around common microplate applications at high throughput. Plate sealing, barcode labeling, microplate stacking, and plate centrifugation along with more advanced liquid handling applications like serial dilutions or plate replication are easily accomplished. While all the different devices can be configured together to automate your specific microplate processing workflow, the PlateLoc Thermal Microplate Sealer can also operate as a standalone fully automated plate sealer. PlateLoc Thermal Microplate Sealer The Agilent PlateLoc Thermal Microplate Sealer is the premier fully automatic thermal heat sealer for the lab. Featuring an intuitive and easy-to-use interface, it accommodates deep-well, assay, PCR, and compound storage microplates with a variety of seals for different applications. This fully automated lab plate sealer is built on a well-established platform with a robust and compact design that is distinguished by a long and proven reputation. The PlateLoc automated microplate sealer is also ideal for robotic integration, featuring an extended-travel plate stage, RS-232 serial port, and ActiveX control.
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delvenservices · 9 months
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Next Generation Sequencing (NGS) Market Growth Opportunities & Key Players
Next Generation Sequencing (NGS) Market, by Product (Consumables, Platforms, Services and Nerve Blockers), Application (Diagnostics, Biomarkers and Cancer, Reproductive Health, Personalized Medicine, Agriculture and Animal Research, and Other Applications), Technology (Sequencing by Synthesis, Ion Semiconductor Sequencing, Sequencing by Ligation, Pyrosequencing, Single Molecule Real Time Sequencing, and Other Technologies), and End User (Academic and Research Centers, Pharmaceutical and Biotechnology Companies, Hospitals and Clinics, and Other End Users) and region (North America, Europe, Asia-Pacific, Middle East and Africa and South America).
Market Overview
The global Next Generation Sequencing (NGS) market size was estimated at USD 11.59 billion in 2023 and is projected to reach USD 29.84 billion in 2030 at a CAGR of 14.47% during the forecast period 2023-2030.
Next-generation sequencing (NGS) is a massively parallel sequencing method that offers scalability, high throughput, and high speed for genome-wide sequencing of nucleotides. Because it involves the preparation of material for the subsequent sequencing reaction, DNA presequencing is one of the most important processes in a common sequencing protocol. Globally, NGS is increasingly integrated into clinical laboratory analysis, testing and disease diagnosis in the healthcare industry. In pharmacogenomics, next-generation sequencing (NGS) is often used to accelerate the drug discovery process.
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The growth of the global next-generation sequencing market is primarily driven by the growth of genome mapping programs; proliferation of next-generation sequencing applications; rising health care costs; and technological advances in sequencing platforms. For example, in 2019, Thermo Fisher Scientific, a world leader in scientific services, launched a new generation platform, the Ion Torrent Genexus System, which provides sample for one-day reporting. Additionally, Agilent Technologies, Inc., an American analytical development and manufacturing company, brought in 2019, we launched the Magnis NGS Prep System, a next-generation sequencing library preparation system designed to perform complex sequencing analyzes at the push of a button. Fetal genetic makeup and configuration can be detected with NGS-oriented tests such as non-invasive prenatal testing (NIPT) and pre-implantation genetic testing (PGT). R&D-related NGS has risen to new heights thanks to investments in NGS technologies worldwide. Growing demand for genome mapping software and increasing use of NGS is fueling market growth
During the COVID-19 pandemic, governments around the world partnered with the private sector to bring NGS technology to market as a potential diagnostic tool. The NGS diagnostic technique was able to determine the genetic sequence of the virus and helped researchers figure out how mutations occur. Next-generation sequencing (NGS) has been used to study COVID-19, making it much easier to trace the origin of SARS-CoV-2.
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Delvens Industry Expert's Standpoint
The pandemic had a considerable impact on businesses globally, with nations like India and China bearing the brunt of the virus's outbreak Declining sequencing costs have increased the use of NGS in disease diagnosis. Various testing kits and testing services are emerging for the non-invasive prenatal testing, cancer diagnosis, genetic testing and more, leading the global market. In addition, active support from the government through product approval and improvement of reimbursements for such diagnostic tests is expected to provide new opportunities for market growth. Additionally, increasing prevalence of chronic diseases, increase in research and initiation of high-budget genomics projects are expected to boost market growth during the forecast period. These factors have led to the expansion of the market and increased demand for Next Generation Sequencing (NGS) solutions.
Key Findings
The application segment is further fragmented into diagnostics, biomarkers & cancer, reproductive health, personalized medicine, agriculture & animal research, and other applications. The biomarker and cancer segment is expected to account for a larger market size during the forecast period due to increase in prevalence of cancer and advancement in technology in next generation sequencing. However, the personalized medicine segment is expected to witness considerable next generation sequencing market growth during the forecast period, due to surge in demand for drug treatment, advancement in R&D in healthcare sector, and rise in awareness for NGS testing in pharmaceutical and biotechnological industry.
The product type segment is further bifurcated into Consumables, Platforms, Services and Nerve Blockers. The consumables segment is the largest market during the forecast period owing to development of technology in molecular genetics and surge in demand for novel pre-sequencing products and kits that execute DNA fragmentation, target enrichment, size selection, and others. However, the services segment is expected to witness considerable next generation sequencing market growth during the forecast period, due to advancement in technology based on SMRT sequencing, and increase in application of cloud computing for NGS data storage.  
The end user segment is further bifurcated into Academic and Research Centers, Pharmaceutical and Biotechnology Companies, Hospitals and Clinics, and Other End Users. Academic & clinical research to account for a larger market size during the forecast period due to increase in application-based usage in these institutes and rise in oncology research. However, the pharmaceutical & biotechnology companies’ segment is expected to witness considerable market growth during the forecast period, due to increase in prevalence of chronic disease, and advancement in R&D in the next generation sequencing market for therapeutics, oncology, and genomics research.  
The market is also divided into various regions such as North America, Europe, Asia-Pacific, South America, and Middle East and Africa. North America is estimated to account for the largest market share during the forecast period. In North America, increasing number of genomics research projects, rising demand for new NGS platforms and growing use of NGS consumables are various factors projected to drive the growth.
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Regional Analysis
North America to Dominate the Market
North America is estimated to account for the largest market share during the forecast period. In North America, increasing number of genomics research projects, rising demand for new NGS platforms and growing use of NGS consumables are various factors projected to drive the growth.
Competitive Landscape
10X Genomics
Agilent Technologies
BGI Group
Bio-Rad Laboratories Inc.
Brooks Automation Inc.
CD Genomics
DNASTAR Inc.
Eurofins Scientific
F. Hoffmann-La Roche Ltd.
Illumina Inc.
Macrogen Inc.
New England Biolabs
Novogene Corporation Inc.
Oxford Nanopore Technologies
Pacific Biosciences of California Inc.
PerkinElmer Inc.
Precigen Inc., (Intrexon Bioinformatics Germany GmbH)
Promega Corporation
Psomagen
Qiagen
Takara Bio
Tecan Trading Group
Thermo Fisher Scientific Inc.
Zymo Research
Recent Developments
In January, 2023, QIAGEN announced a strategic partnership with California-based population genomics leader Helix to advance next-generation sequencing companion diagnostics in hereditary diseases.  
In March, 2023, The company SOPHiA GENETICS announced a new partnership with Qiagen that will pair QIAseq reagent technology with the DDM platform to enhance tumor analysis through next-generation sequencing (NGS).
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Reasons to Acquire
Increase your understanding of the market for identifying the most suitable strategies and decisions based on sales or revenue fluctuations in terms of volume and value, distribution chain analysis, market trends, and factors.  
Gain authentic and granular data access for the Next Generation Sequencing (NGS) Market to understand the trends and the factors involved in changing market situations.  
Qualitative and quantitative data utilization to discover arrays of future growth from the market trends of leaders to market visionaries and then recognize the significant areas to compete in the future.  
In-depth analysis of the changing trends of the market by visualizing the historic and forecast year growth patterns.
Report Scope
The Next Generation Sequencing (NGS) Market is segmented into various segments such as product, application, technology, end-user and region:
Based on Product
Consumables  
Sample Preparation Consumables  
DNA Fragmentation, End Repair, A-Tailing and Size Selection
Library Preparation & Target Enrichment
Quality Control
Other Consumables  
Platforms
HiSeq
MiSeq
Ion Torrent
Solid
Pacbio Rs II and Sequel System
Other Sequencing Platforms
Services  
Sequencing Services  
RNA Sequencing
Whole Exome Sequencing
Whole Genome Sequencing
Targeted Sequencing
Chip Sequencing
De Novo Sequencing
Methyl Sequencing
Data Management Services  
NGS Data Analysis Services
NGS Data Analysis Software & Workbenches
NGS Storage, Management and Cloud Computing Solutions
Based on Application
Diagnostics
Biomarkers and Cancer
Reproductive Health
Personalized Medicine
Agriculture and Animal Research
Other Applications
Based on the Technology
Sequencing by Synthesis
Ion Semiconductor Sequencing
Sequencing by Ligation
Pyrosequencing
Single Molecule Real Time Sequencing
Other Technologies
Based on End User
Academic and Research Centers
Pharmaceutical and Biotechnology Companies
Hospitals and Clinics
Other End Users
About Us:
Delvens is a strategic advisory and consulting company headquartered in New Delhi, India. The company holds expertise in providing syndicated research reports, customized research reports and consulting services. Delvens qualitative and quantitative data is highly utilized by each level from niche to major markets, serving more than 1K prominent companies by assuring to provide the information on country, regional and global business environment. We have a database for more than 45 industries in more than 115+ major countries globally.
Delvens database assists the clients by providing in-depth information in crucial business decisions. Delvens offers significant facts and figures across various industries namely Healthcare, IT & Telecom, Chemicals & Materials, Semiconductor & Electronics, Energy, Pharmaceutical, Consumer Goods & Services, Food & Beverages. Our company provides an exhaustive and comprehensive understanding of the business environment.
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thedevilworkshard · 5 years
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Collection of accumulated college advice
I’m not a college student yet but I am an incoming freshman at Ateneo de Manila University here in the Philippines (however, UP hasn’t released their admission results yet so you never know if I’ll be an Isko ng Bayan). I got into BS ITE (Information Technology Entrepreneurship) which is basically a collab of computer science and business. It’s really heavy on entrepreneurship and business management. The goal of the course is to have a startup by the time you graduate so it’s a quota program and overall a solid course.
Enough talking/reading. Here’s my compilation of college tips that I’ve compiled from Youtube binging, Tumblr surfing, and Pinterest browsing.
Basic Advice
Enroll in a Coding elective/class or get a tutor who can teach you basic coding/programming. It doesn’t even have to be coding, but honestly the skill will probably help in the long run.
Invest in make-up wipes. Keep a stock by your bedside table at all times.
Invest in a 500mL to 1L jug that you can carry at all times but also retains temperature well. Don’t get a hydroflask (it’s not worth it). 
Invest in earphones, or just a good headset (perhaps Airpods if you have the money).
Start waking up early 1 month before classes start. That way, you can easily transition into going to class early and you can claim your seat at each class. 
Invest in a good alarm clock or at least a fair waking up system.
Make a website and create a LinkedIn account. This will help with internships.
Start making categoric folders for your email (Specific orgs, Professor/To-professor, Temporary, Keep, etc.)
Get the syllabus and write down all the due dates. Make copies of the syllabus (one for hard copy and one for soft copy).
Budget your allowance. Reserve an hour or two a day for self-care like exercise, napping, Netflix, etc.
Write textbook notes on paper that is the same size if not smaller than your notebooks so you can paper clip it on or binder clip them all.
Take note of all extra-curricular projects, and even big academic ones. Write them down, especially the dates of when they started and when they ended. This is for the resume.
Email professors or ask them in person if the subject textbook will deem useful in discussion/tests.
Join Aegis (if you go to Ateneo). They handle yearbook photos.
Supplies Advice
Rubber shoes (invest in ones that are super durable)
1 x 1 ID pics (stockpile dude)
3 x 5 index cards (apparently, Ateneans use these a lot)
USB/Hard drive
a jAcKeT (weather in a classroom often varies from outside the classroom)
Earphones (as said previously)(I swear just get a good pair/set)
Ultimate Notebook Hack
This is my plan for my note-taking + study system so you may adopt it if you think it will suit your learning techniques.
Assignment notebook
Characteristics:  thick, spiral bound, dividers for classes
Purpose:  assignments, exercises, practice, problems, essays, etc.
Brand: Typo spiral notebook
Contemporary notebook
Characteristics: durable, aesthetically pleasing 
Purpose: in-class notes, can be messy, prone to erasures, bring everywhere
Brand: Fully booked spiral notebook
Master notebook
Characteristics: binder, detachable pages
Purpose: clean + understandable notes, final notes on lessons with ALL information, for exam prep (finals)
Brand: MUJI binder notebook
Where to Study
I don’t have only one study technique. I like to change it up every once and a while. Like today study group or study partner, but tomorrow study at home. Maybe next week, study in a classroom but the next day, study in a cafe. Maybe I like rewriting notes or I like to read from the book. It really depends on my mood. I don’t know a lot of people who are like this but I made a list of places that I can easily study at. Productivity is really hard when you don’t have the right sources of motivation so hang in there and experiment with different places/techniques.
For study groups
When you feel like someone’s productivity will infect you
When you want to teach someone (to master the lesson more)
Cafes or empty classrooms
You must be open to noise and discussion
NEVER more than 2
Always with people that you trust to be quiet
Long table (you don’t want to feel cramped)
For studying alone (with motivation)
quiet, well-lit corner in the library
at home
cafes
bring snacks if you’re not home
if you like music, listen to classical compositions or opera
For studying alone (without motivation)
coworking spaces (Cospace along Katipunan)
empty classrooms (that are allowed to be stayed in after school hours)
For studying in a place that doesn’t have a studious atmosphere
trust me, you’ll end up stuck in a place that isn’t meant for studying like the car, a family dinner, in the mall shopping with whomever. you have an exam or an assignment due the next day.
if you’re permitted, record your professor’s lectures and listen to it using earphones
download quizlet on your phone and find flashcards regarding your lesson
write on a receipt everything you know about the topic. when you get home to your notes, check if you’re right. try to rewrite it with the correct info
if you’re in the car, sleep
watch videos online explaining a topic (whether you get it or not)
crashcourse is a godsend. thank you hank green
This is pretty much a master post? I got inspo from a lot of Youtubers and studyblrs and Pinterest pins, but most of these are from my last year of senior high school (which in the Philippines, is basically a preview of the first semester of freshman year in college).
If anyone is in the same boat (BS ITE, incoming freshman, excited about college) or not even (incoming senior, taking admission exams), feel free to message me. I’m open to trying to figure out all that s***.
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evoldir · 1 year
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Fwd: Job: ClemsonU.LabTech.GenomicsBioinformatics
Begin forwarded message: > From: [email protected] > Subject: Job: ClemsonU.LabTech.GenomicsBioinformatics > Date: 13 January 2023 at 07:00:39 GMT > To: [email protected] > > > We are looking to hire a Lab Research Specialist with a focus in > molecular biology and lab management at the Clemson University Genomics > and Bioinformatics facility. > > For more information: [email protected] > > https://bit.ly/3GJaHmm > > > JOB SUMMARY: > Laboratory Research Specialist will assist in day-to-day lab management > duties, supervise and train student and postdoctoral researchers, conduct > experiments, perform basic bioinformatic analyses, conduct other genomic > related research activities and perform other duties as assigned. > > JOB DUTIES: > 55% - Essential - Research Activities: Performs technical molecular > biology experiments, including but not limited to: DNA/RNA isolation, > cDNA prep, RNA/DNA library preparation, Illumina short-read NGS, qPCR, > PCR, etc. Performs basic bioinformatics tasks such as de-multiplexing, > trimming NGS reads, etc. Oversees and runs the Agilent Bioanylzer, > the Illumina NGS sequencing platforms and other equipment. > > 30% - Essential - Lab Management:  Manages lab ordering, invoicing, > budgeting, record keeping and generation of quotes. Assists > in maintaining regulatory compliance. Manages lab inventory and > records. Maintains/Updates social media/website and helps organize > seminars/workshops. Oversees day-to-day maintenance of lab space, > facilities, and equipment. > > 15% - Essential - Personnel Oversight:  Assists in training personnel, > students, staff, and faculty levels.  Assists in providing oversight > for student researchers. > > MINIMUM REQUIREMENTS: > Education -  Bachelor's Degree - Biology or related field > Work Experience 2+ years > > > > > > Christopher L. Parkinson > Department of Biological Sciences, and > Department of Forestry and Environmental Conservation > Director, Clemson University Genomics and Bioinformatics Facility > 190 Collings St., Life Sciences Facility, Clemson University, Clemson, > SC 29634 > > Christopher L Parkinson
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articlepublication · 2 years
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Looking to improve NGS Sample Prep?
NGS (Next-Generation Sequencing) has aided rapid strides in genomics data generation but getting to the results may prove labor and time-consuming. Sample prep can be complicated if samples are damaged or degraded. However, experts recommend the following tips to accelerate and simplify the sample prep.
Accept that you will hit bottlenecks
A sequence may have a natural variation or the variation may result from the NGS workflow. There are different steps in sample prep and any of them could be responsible for variation, for example, inaccurate pipetting can impact data. After a long NGS process, if you find that you have bad data, you would have to start again. This means more sample requirements as well as added reagent and labor costs. This is why NGS sample prep needs to be consistent and repeatable.
Look for NGS Sample Prep Tools
Find the right NGS sample prep products for your research so that you can eliminate the external sources of variability and inconsistency in your workflow, and can be more assured of true results.
Generally, the biggest challenges in NGS sample prep include:
throughput
many sample-prep steps
hands-on time
human error
cost per sample
cooling of samples and reagents
finding the right kit for a specific application
Specific challenges for sample prep upstream of long-read sequencing include extracting high molecular weight DNA quickly and easily. The most widely used Phenol extraction is inconvenient and labor-intensive.
Handling high molecular weight DNA is complex. It does not mix homogeneously into the solution easily and is likely to yield inaccurate quantitation and purity assessment. But some applications need longer DNA fragments. Hence, it is important to use high-quality input material, as well as an extraction workflow that keeps nuclease activity and DNA shearing to a minimum.
How to fix it?
Automation is essential for standardizing complex NGS sample-preparation workflows. Compared to manual pipetting, liquid-handling workstations provide more reproducibility and accuracy. Many automated workstations support multiple library protocols for added flexibility.
Automation can be added in various ways –
semi-automation in nucleic-acid extraction, clean-up steps, size selection, adapter ligation, enrichment with an automated liquid-handling solution
full automation for the execution of the whole library prep workflow
If you are looking for high-quality magnetic bead-based kits for nucleic acid isolation and sample collection and stabilization tubes, call one of the most trusted RNA extraction kit manufacturers in the USA, MagBio Genomics at (301) 302-0144.
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