Tumgik
#anti-kda
raveneira · 10 months
Text
'Ada deserves better than Kawaki'
The worst thing Kawaki has done to Ada
Tumblr media Tumblr media
The worst things Ada has done to Kawaki
Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media Tumblr media
The worst Kawaki ever did to Ada was grab her collar to force her to lie which she could've disobeyed, thats it.
The worst things Ada did to Kawaki is
Trade everybody important to Kawaki in exchange for Code to spare him so she can pursue him, essentially human trafficking according to Bug, which is a horrible experience Kawaki's already gone through
Asking Code to spare Naruto after seeing how much he meant to Kawaki but when he refuses she says fine and to just not let Kawaki know shes involved because she dont want him to hate her, but could care less about the pain losing Naruto would have on him
She could've sent Daemon to intervene when Momo was brutally beating Kawaki and then about to kill Naruto right infront of him yet she sat there watching the whole thing and never tried to help despate literally watching in real time the despair Kawaki was in watching Naruto about to die.
She says Codes goals have nothing to do with her and that shes only helping him in exchange for Kawaki, yet when Shikamaru offers to make that happen without Code she still chooses to leave with Code and help him get his limiters off knowing how difficult that'd make things for everyone he cares about because she knows in the end they'd have to rely on her and Daemon for protection which'll give her access to Kawaki in exchange for that protection
Forced him on glorified house arrest
She holds the entire village hostage by the fact that they need Daemons strength and Ada's sight to deal with Code in exchange for getting to live with Kawaki against his will, Kawaki is not asked for his consent about ANY of this and it was all a deal that Amado and Shikamaru made with her on his behalf and she did not care as long as she's benefiting from Kawaki's trafficking
She allows her brother to beat Kawaki unconscious UNINTERUPPTED when they first meet and does not apologize to him for it
When Kawaki confronts her about her partnership with Code and correctly deduces that she sold Boruto out in exchange for him, Ada doesnt feel any remorse for it nor does she apologize for her selfishness, instead she changes the subject altogether and doesnt even address it
Even though it was unintentional she made omnipotence happen and completely miscontrued what Kawaki wanted by making him take Boruto's place entirely when all he actually wanted, and clearly stated, was for Boruto to be seen as an outsider so that nobody would interfere or feel bad when he kills him
Because of her now Kawaki is being forced to live a miserable life with everyone treating him like Boruto when he doesnt want that and also listening to ppl talk about how much they actually hate him and want him dead everytime they talk about Boruto who they really mean Kawaki when they say those cruel things. Meanwhile Ada is at home chillin without a care in the world about how her mess has affected him, infact she just suggests giving up on trying to undo it despite supposedly feeling bad for making Kawaki seem like a coward.
STFU
Ada does NOT deserve better, KAWAKI does, from the moment she's been introduced, literally the MOMENT she was introduced she didnt give a danm about Kawaki's feelings or consent AT ALL and made multiple deals disregarding the ppl he cares about with 3 different ppl in exchange for him like he wasnt even a human being but a piece of property to be bought and sold.
She felt zero remorse, I repeat, she felt ZERO remorse about working with Code who nearly killed Naruto right infront of him, and sold his brother out in exchange for him, when confronted about it by Kawaki himself and she has the chance to apologize she DOESNT do it, not just there, but she NEVER owns up to the fked up things she did nor apologizes to him for it, yet she apologizes to Boruto multiple times despite Kawaki being the one she supposedly loves.
How anybody, and I mean ANYBODY thinks Ada's the victim here is beyond me, even more so how anyone thinks Kawaki would ever even CONSIDER being together with her after all this when he himself has said she's scum and that he would've rathered taken his chances with Code than even meet her, 3 years have passed and Ada is still sitting home alone while Kawaki completely ignores her existence, how any of you think this ship has ANY possibility of happening is laughable, its even worse than KawaSumi's chances and theirs are low as hell too, but even that has more of a chance than Ada ever will.
So like I said, stfu, Ada doesnt deserve better, Kawaki does, if anything Ada deserves WORSE because she has gotten off scott free with no repercussions for way too long.
30 notes · View notes
babs-babbles · 1 year
Note
omg spiderverse kda yes pleaseeeeeeee
Okok so I got it!! Kda spiderverse au where Akali is this universes spiderwoman
Akali: Ninja Spider, her dimension's one and only spider person. When not suited and saving lives she's DJ's underground clubs. One night while packing away her supplies after a gig she got bit by a radioactive spider, and since then has been using her powers to help the people of Runterra City
Kai'sa: human bonded to a symbiote and this dimension's resident venom. Misunderstood anti hero who's trying her best. By day she's disguised as a local dance instructor, by night she hunts down bad guys that slip through the cracks. Pairs up with Akali on rare occasions for the really hard to get villains
Ahri: radio show host and "reporter". This dimension's "MJ"(she lives) and "Ned". She helps Akali by tracking police radios and keeping investigators off her tail. Often tells Akali that thanks to her natural charms no one suspects a thing. But don't let the sweet talk fool you, she might be hiding something
Evelynn: Definitely the dimension's Kingpin. Or at least...the women behind him. Eve runs most of the crime and such going on in Runterra City, from smuggling goods, organized gangs, the occasional missing persons. All her. Under the disguise of the original Kingpin, who she took out to take control over everything
I only know the basics and such about the marvel universe(s). But I've always liked Spiderman and the movies were definitely my favorite growing up. Combine that with my love for kda and we get cool shiz like this. Might draw up some spiderverse kda soon
33 notes · View notes
waddinghamhannah · 1 year
Text
i was tagged by @casismybestfriend and several other people honestly. Thanks! 💜
rules: spell your url with song titles and then tag as many people as there are letters.
W: Where Do We Go - Lindsey Stirling
A: A Little Messed Up - june
D: DogBird - Madds Buckley
D: Dial Drunk - Noah Kahan
I: If It Ain't Love - Jason Derulo
N: No Way Out - Divide Music
G: Good For You - Dear Evan Hansen
H: High - Sivik
A: Applause - Lady Gaga
M: MORE - KDA
H: HAPPY - NF
A: Already Over - Sabrina Carpenter
N: Nothing Ever Changes - Nico Collins
N: NO - Megan Trainor
A: Anti-Hero - Taylor Swift
H: Hurricane - Panic! at the Disco
(I had some help so if you see this thank you! 💜)
Tagging: @benoitblanc , @5chatzi , @modern-day-classic , @movrings , @twoheartsoneclara , @nuttmeg13 , @eohwyyn , @first-kanaphan , @jcusack @binickmiller , @wednesday-adams , @gilliandersons, @doctorbeverlycrusher, @queerhydrangea , @revolutionsoftheheart , & whoever wants to do this!
11 notes · View notes
moleculardepot · 23 days
Text
Mouse Anti Human 515 kDa LRP Biotin Labeled
Mouse Anti Human 515 kDa LRP Biotin Labeled Catalog number: B2017872 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 μg Molecular Weight or Concentration: N/A Supplied as: Solution Applications: a molecular tool for various biochemical applications Storage: -80°C Keywords: Biotin labeled Anti Human 515 kDa LRP Grade: Biotechnology grade. All products are highly pure. All…
0 notes
militaryleak · 4 months
Text
KONGSBERG Diehl and MBDA to Develop Super Sonic Strike Missile (3SM) Tyrfing
Kongsberg Defence & Aerospace (“KONGSBERG”), along with its German partners Diehl Defence and MBDA Deutschland, have teamed to ensure the successful development of the future Norwegian and German super-sonic strike missile known as 3SM Tyrfing. The missile is expected to be delivered from 2035 and is planned to be used on Norwegian and German naval vessels. The missile will complement the Naval Strike Missile. The Naval Strike Missile (NSM) is an anti-ship and land-attack missile developed by the Norwegian company Kongsberg Defence & Aerospace (KDA). The name Tyrfing originates from Norse mythology, where it was a sword that always hit what it was swung at, could never rust and could cut through stone and iron as easily as through clothing.
Kongsberg Defence & Aerospace (“KONGSBERG”), along with its German partners Diehl Defence and MBDA Deutschland, have teamed to ensure the successful development of the future Norwegian and German super-sonic strike missile known as 3SM Tyrfing. The missile is expected to be delivered from 2035 and is planned to be used on Norwegian and German naval vessels. The missile will complement the Naval…
Tumblr media
View On WordPress
0 notes
tiquila-louise-moist · 6 months
Text
How Gepard System Terminates Kamikaze Drones in Ukraine?
Girl Gang #lollypop500joules #millionaire #billionaire #trillionaire #septillionaire
0 notes
hypeptide · 9 months
Text
What are the advantages of peptide drugs and what are the current types of peptide drugs?
Peptides hold a unique position in the field of drug development, and since the emergence of therapeutic insulin in 1922, peptides have played an important role in medical practice. So far, more than one hundred polypeptide drugs have been approved for marketing in the world, and are widely used in the treatment of diabetes, tumor, chronic pain, multiple sclerosis and other diseases. Previously, we have introduced the origin and development of peptides and peptide drugs, and explained the classification and screening of peptide drugs (see previous tweets: Peptides – Unique Drugs).
Today, let’s have a hardcore science popularization on peptide drugs!
1.Peptides, what are their advantages?
Peptides are molecules composed of amino acids as the basic unit, with a molecular weight generally below 10 KDa, between small chemical molecules and biological products. Their main characteristics are high selectivity and low concentration of action. Classic therapeutic peptides such as hormones, growth factors, ion channel ligands, etc. trigger intracellular effects by binding to receptors.
Compared with biological agents such as antibodies and therapeutic proteins, peptides have a similar mode of action, but their immunogenicity is lower. Additionally, due to their ability to be chemically synthesized, their production cost is also lower.
Tumblr media
2.What are peptide drugs available?
2.1 Hormonal peptides and their derivative drugs
Generally speaking, there are four main medicinal peptides in clinical practice, one of which is hormone peptides and their derivatives. Due to the short half-life and high synthesis cost of peptides, early peptide drug development mainly focused on the field of low concentration human hormone peptides. The research on peptide drugs began with insulin, followed by the emergence of short peptide drugs such as oxytocin, antidiuretic hormone, somatostatin, and gonadotropin-releasing hormone, which opened up and enriched this field (Table 1). Many hormone drugs are still in use today.
With continuous scientific research, people have improved the characteristics of peptide hormones through chemical modifications such as C-terminal amidation, D-type amino acids, cyclization, and conjugation of long-chain fatty hydrocarbons.
For example, Octreotide and Pasireotide, which are based on somatostatin β Transforming the pharmacophore for modification successfully extended its half-life. In addition, drug development can also be based on the development of agonists or antagonists related to peptide hormones, such as Goserelin and Cetrorelix [3].
Tumblr media
2.2 Natural Peptide Products
Tumblr media
2.3 Peptide vaccines
Of course, there is also a peptide vaccine that must be mentioned. It is a subunit vaccine made from peptides, which can act as an immunogen to stimulate the body to produce an immune response by simulating the epitope sequence of antigens. Multimeric001, a representative type of anti infective peptide vaccine, contains epitopes of influenza virus B, T helper cells, and cytotoxic T cells, which can prevent various types of A and B influenza viruses. It has entered clinical stage III [5].
Tumblr media
The antigenic peptide IMDQVPFSV (Disaotide) that binds to the natural epitope ITDQVPFSV after point mutation exhibits stronger immunogenicity.
2.4 PDC peptide drugs
Tumblr media
3.Summary
This issue introduces the characteristics of peptide drugs and four main types of medicinal peptides, and combines specific cases to further deepen our understanding of peptide drugs. At present, peptide drugs have been in clinical practice for a century, and classic hormone drugs still dominate the main market. The two major bottlenecks of inconvenient delivery methods and frequent delivery cycles still need to be solved urgently. The constantly emerging new technologies such as PDC and multifunctional peptides also need to be explored and enriched by friends~
The advantage of the KS-V Peptide integration service platform is to launch a catalog of peptide products with scientific research value. Each peptide product is purified by HPLC, resulting in more stable quality and timely delivery. The application scenarios cover new hotspots and valuable research fields, such as protein purification and detection, disease-related research, immunology and biochemistry research, scientific research peptides, medicinal peptides, etc., to meet the needs of researchers at different stages. We have a complete customer service system and technical team, enjoying a one-on-one specialized service experience, and providing you with professional services in a timely manner.
0 notes
kaira-moon · 1 year
Text
Most Effective Essential Oil for Nail Fungus: Tea Tree Oil and Metanail Complex
Tumblr media
Nail fungus is a common and often persistent issue that affects a significant portion of the population. If you're dealing with this problem, you're not alone. In this article, we explore two potent solutions for nail fungus - Tea Tree Oil and Metanail Complex, focusing on the most effective essential oil for nail fungus.
Tea Tree Oil: Nature's Fungus Fighter [ Most Effective Essential Oil for Nail Fungus? ]
Tumblr media
Tea Tree Oil, derived from the leaves of the Australian tea tree (Melaleuca alternifolia), is celebrated as one of the most effective essential oils for nail fungus. This natural remedy has gained recognition for its antifungal and antibacterial properties. It contains compounds like terpinen-4-ol that are known to combat fungal infections effectively.
Why Tea Tree Oil Is Effective Against Nail Fungus
Antifungal Properties: Tea Tree Oil is renowned for its potent antifungal activity, making it an ideal choice for tackling nail fungus. It can penetrate the nail bed to target the fungus directly.
Antiseptic Action: In addition to fighting the fungus, Tea Tree Oil helps prevent secondary infections and promotes overall nail health.
Anti-Inflammatory: It reduces inflammation and soothes the affected area, providing relief from discomfort.
Natural and Safe: Tea Tree Oil is a natural remedy without the potential side effects associated with some medications.
How to Use Tea Tree Oil for Nail Fungus
To harness the benefits of Tea Tree Oil:
Mix a few drops of Tea Tree Oil with a carrier oil like jojoba or coconut oil.
Apply the mixture to the affected nails and surrounding skin.
Allow it to soak in for about 10-15 minutes.
Gently scrub the nails with a soft toothbrush.
Repeat this process daily until the fungus clears up.
Metanail Complex: A Faster Comprehensive Solution [ Most Effective Essential Oil for Nail Fungus? ]
Tumblr media
While Tea Tree Oil is a powerful natural remedy for nail fungus, some individuals prefer a comprehensive solution. This is where Metanail Complex, the most effective essential oil for nail fungus, comes into play.
Metanail Complex incorporates Tea Tree Oil along with a range of other natural ingredients, offering a synergistic approach to fighting nail fungus. Its unique formulation ensures that you're not just treating the symptoms but also addressing the root cause of the issue.
Click here to learn more about Metanail Complex.
Metanail Complex Ingredients:
Tumblr media
Witch Hazel, Scots Pine, and Horsetail Extract: A powerhouse trio compound that works wonders for your nails and skin.
Gotu Kola: Supports the skin's natural protective function.
Rosemary & Pelargonium Graveolens: To gently nourish the skin & moisturize nail cuticles.
Glycerin: To hydrate the outer layer of the skin and soften the skin.
Lemon Peel Extract & Aloe Vera: Organic Green Tea & Hops: Loaded with the active protein known as 14 kDa, which harbors powerful nail rejuvenating properties.
Vitamin C and Vitamin E: Essential for the production of collagen, the building block of fingernails, which gives them shape, strength, and integrity.
Hyaluronic Acid: Has fantastic properties when it comes to naturally reinforcing nail integrity.
Jojoba Seed Oil and Sage Leaf Extract: Uniquely effective when it comes to long-term nail and foot comfort.
MSM: Highly praised by modern science for its strengthening properties.
The Metanail Advantage: Faster Results
One distinct advantage of Metanail Complex is its ability to deliver faster results. Users have reported remarkable improvements in record time. The carefully selected ingredients work in perfect harmony to accelerate the healing process, allowing you to regain healthier and more beautiful nails sooner.
Click here to learn more about Metanail Complex.
Conclusion: Your Solution for Nail Fungus
According to the latest research, in the United States alone More than half of all Americans are symptomatic of highly contagious, unsightly, and often difficult-to-cure nail fungus and infections, and may potentially pass them along to others by sharing nail tools, according to a new survey on nail hygiene habits. That means over 100 million American adults are in need of an anti-fungal solution.
In a country where nail fungus affects millions, you have choices. Tea Tree Oil stands as a proven, natural remedy, while Metanail Complex offers a holistic approach with a blend of essential oils and natural ingredients, including a speed advantage that makes it the most effective essential oil for nail fungus. Whether you opt for the power of Tea Tree Oil or the convenience of Metanail Complex, you're taking a significant step toward healthier and more beautiful nails.
Say goodbye to nail fungus and hello to confidence and comfort with your chosen solution. It's time to regain control over your nail health.
Click here to learn more about Metanail Complex.
0 notes
usafphantom2 · 2 years
Text
Tumblr media
USAF confirms acquisition of JSM cruise missiles for its F-35
Fernando Valduga By Fernando Valduga 23/03/2023 - 18:00 in Armaments, Military
The U.S. Air Force plans to buy 48 JSM (Joint Strike Missile) cruise missiles for its F-35 Lightning II fighters, according to a budget request for fiscal year 2024.
The cruise missile is developed by Kongsberg Defense & Aerospace (KDA) of Norway and Raytheon.
The JSM was designed to target high-value targets on land and at sea, such as ships, radar sites, command centers and air defenses. It has a range of more than 300 km (186 miles) and can fly at low altitudes to avoid detection by enemy radars. It also has a high degree of maneuverability and can change course during flight to avoid enemy countermeasures.
Tumblr media
The JSM during integration tests with the F-35's internal weapons compartment.
The JSM is based on the NSM (Naval Strike Missile), which has been widely adopted by several navies around the world as an anti-ship missile. The JSM is modified to fit inside the F-35's internal weapon compartments, reducing its radar signature and increasing its stealth capabilities. The JSM can also be transported externally by other aircraft, such as F-16 and F/A-18.
The U.S. Air Force is expected to pay about $3.35 million per missile, based on an estimate derived from previous contracts signed by Norway and Japan, which are also JSM customers. Norway is the leading nation in the development of the JSM and has ordered 140 missiles for its own F-35. Japan also ordered 37 missiles for its F-35.
Tumblr media
JSM tests with F-35A fighter.
The U.S. Air Force has been testing the JSM since 2018, when it conducted the first release test with an F-35A at Edwards Air Base, California. In February 2021, he conducted another release test with an F-35A in the Utah Test and Training Range. The tests showed that the missile can be safely launched from the aircraft and function as expected.
The U.S. Air Force plans to declare initial operational capability (IOC) for the JSM in 2025, which means it will have enough missiles and trained personnel to carry out missions with them. The IOC will coincide with the decision to produce a full rate for the missile, which will allow larger orders in subsequent years.
Tumblr media
The JSM will provide a significant boost to the U.S. Air Force's attack capabilities, especially in contested environments where access can be denied by sophisticated enemy defenses. The JSM will complement other cruise missiles launched from the air in service or development by the U.S. military, such as the AGM-158 JASSM (Joint Air-to-Surface Standoff Missile) and the AGM-179 JSOW-C1 (Joint Standoff Weapon).
The acquisition of JSM cruise missiles by the U.S. Air Force reflects its commitment to modernize its weapons portfolio and maintain its advantage over potential opponents. JSM will also increase interoperability with allies who share common platforms and threats.
Tags: weaponsMilitary AviationF-35 Lightning IIJSM - Joint Strike MissileUSAF - United States Air Force / U.S. Air Force
Fernando Valduga
Fernando Valduga
Aviation photographer and pilot since 1992, he has participated in several events and air operations, such as Cruzex, AirVenture, Dayton Airshow and FIDAE. He has works published in specialized aviation magazines in Brazil and abroad. Uses Canon equipment during his photographic work throughout the world of aviation.
Related news
HELICOPTERS
Paramount will produce in the United Arab Emirates the rotor blades of Mi-type helicopters
23/03/2023 - 14:00
MILITARY
IMAGES: Polish, Dutch and French fighters train on the Eastern European flank
23/03/2023 - 12:00
MILITARY
Russian forces use DJI drones made in China to acquire targets
23/03/2023 - 09:00
MILITARY
Lockheed Martin invests in Regent Craft's Seagliders for military uses
23/03/2023 - 08:24
MILITARY
First flight of the B-21 is “a few months” delayed
22/03/2023 - 22:57
HELICOPTERS
U.S. offers AH-1Z Vipers and Hellfire missiles to Slovakia, compensating MiG-29s donated to Ukraine
22/03/2023 - 19:12
Cavok Twitter
homeMain PageEditorialsINFORMATIONeventsCooperateSpecialitiesadvertiseabout
Cavok Brazil - Web Creation Tchê Digital
Commercial
Executive
Helicopters
HISTORY
Military
Brazilian Air Force
Space
Specialities
Cavok Brazil - Web Creation Tchê Digital
0 notes
urduhindipoetry · 2 years
Text
CHARCO - Skin To Internal Health's Hyaluronic Acid Powder, Cosmetic Grade "Molecular W. : 800 kda" ( Quantity :10 Gm) For Serum Making, Anti Ageing Beauty Formulations, Moisturizer & Lotion Making, & DIY Personal Care For Face, Hair, Skin & Body
CHARCO – Skin To Internal Health’s Hyaluronic Acid Powder, Cosmetic Grade “Molecular W. : 800 kda” ( Quantity :10 Gm) For Serum Making, Anti Ageing Beauty Formulations, Moisturizer & Lotion Making, & DIY Personal Care For Face, Hair, Skin & Body
Price: (as of – Details) Product Description Intense hydration Hyaluronic acid is well known for its outstanding moisture retaining ability and viscoelastic properties that can help keep your skin hydrated at all times. Effective ingredient Hyaluronic acid is effective by locking in hydration on the layer of the skin, therefore improving the skin texture and appearance. Skincare ingredient A…
Tumblr media
View On WordPress
0 notes
moleculardepot · 26 days
Text
Mouse Anti Human 515 kDa LRP
Mouse Anti Human 515 kDa LRP Catalog number: B2017806 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 1 mg Molecular Weight or Concentration: N/A Supplied as: Solution Applications: a molecular tool for various biochemical applications Storage: -80°C Keywords: Anti Human 515 kDa LRP Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I…
0 notes
reporterpiner · 2 years
Text
Lzip pgc1a
Tumblr media
Membrane Blocking and Antibody Incubations Electrotransfer to nitrocellulose membrane ( #12369).Ĭ.NOTE: Loading of prestained molecular weight markers ( #59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder ( #7727, 10 µl/lane) to determine molecular weights are recommended. Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm). Heat a 20 µl sample to 95–100☌ for 5 min cool on ice.Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate).Aspirate media from cultures wash cells with 1X PBS aspirate.Treat cells by adding fresh media containing regulator for desired time.Protein Blotting A general protocol for sample preparation. Detection Reagent: SignalFire™ ECL Reagent ( #6883).ī.Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody ( #7074).Pore size 0.2 µm is generally recommended. Blotting Membrane and Paper: ( #12369) This protocol has been optimized for nitrocellulose membranes.Blue Prestained Protein Marker, Broad Range (11-250 kDa): ( #59329).Biotinylated Protein Ladder Detection Pack: ( #7727).Primary Antibody Dilution Buffer: 1X TBST with 5% BSA for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.10X Tris Buffered Saline with Tween ® 20 (TBST): ( #9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH 2O, mix.10X Tris-Glycine Transfer Buffer: ( #12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH 2O, mix.10X Tris-Glycine SDS Running Buffer: ( #4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH 2O, mix.1X SDS Sample Buffer: Blue Loading Pack ( #7722) or Red Loading Pack ( #7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer.10X Tris Buffered Saline (TBS): ( #12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH 2O, mix.20X Phosphate Buffered Saline (PBS): ( #9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH 2O, mix.NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water. Solutions and Reagentsįrom sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit NOTE: Please refer to primary antibody product webpage for recommended antibody dilution. For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4☌ with gentle shaking, overnight.
Tumblr media
0 notes
Text
Tumblr media
2 notes · View notes
o-antigravity-o · 6 years
Text
Tumblr media
Neon K/DA Akali wip
Oof =~=(?)
4 notes · View notes
wyrmguardsecrets · 3 years
Note
How many of you yelling and screeching at people to quit wow are getting DA4? How many of you play KDA songs on repeat? How many sub to final fantasy? Congratulations, you are supporting companies that exploit and harass women. A systemic issue isn't going to change across a whole industry because you unsub. Read a book and find actual organizations to support. GWU and your local anti discrimination chapters are a good start.
4 notes · View notes
violetcaterpillar · 3 years
Note
Anty tol alw2t betndby kda lih? Wy tol alw2t 3ysha dor d7ya lih?
والله حضرتك ده البلوج بتاعي بكتب فيه اي حاجة عجباني او حسيتها، مش شرط كل حاجة بكتبها اكون قاصدة بيها حد او انا بمر بيها شخصيا، وانا اصلا معرفش ناس كتير هنا فمعتقدش اللي يعرفني كويس حيقول كده و مش مستنية قبول او رفض من حد فلو حضرتك مضايق او مضايقة ممكن يمنتهي السهولة تعملي unfollow و حكون ممنونة جدا :)
3 notes · View notes