#protein purification

[inscinstech]

Protein Purification | Inscinstech.com.cn

Unlock the full potential of your proteins with Inscinstech.com.cn advanced protein purification technology. Experience unparalleled results today!

protein purification

[captainsbestgal]

This is gonna be a niche ass post, but I wanna brag about my protein purification. Look at that pure ass protein.

[seoexpertwahid1]

The powering performance unleashing the potential of protein supplements

In the world of fitness and sports, athletes and enthusiasts are constantly searching for ways to optimize their performance and achieve their goals. One popular tool that has gained significant attention is protein supplements. These products have become synonymous with muscle growth and recovery, but their benefits extend far beyond the realm of bodybuilding. In this blog post, we will delve into the potential of protein supplements and explore how they can enhance performance across various domains.

Understanding Protein and Its Role in the Body: To grasp the significance of protein supplements, it's essential to understand the role of protein in the body. Proteins are the building blocks of tissues and play a vital role in muscle repair, growth, and overall cellular function. They are composed of amino acids, which are essential for numerous physiological processes. By consuming protein supplements, individuals can ensure an adequate supply of these essential nutrients.

Muscle Growth and Recovery: One of the primary benefits associated with protein supplements is their ability to support muscle growth and expedite recovery. During intense exercise, muscle fibers undergo microtears, and consuming protein post-workout helps repair and rebuild these fibers. Protein supplements, such as whey protein or casein protein, provide a convenient and easily digestible source of amino acids, promoting efficient muscle recovery and hypertrophy.

Enhancing Athletic Performance: Protein supplements are not limited to bodybuilders alone; athletes from various disciplines can also benefit from their consumption. Proteins are involved in energy production, endurance, and overall athletic performance. By incorporating protein supplements into their regimen, athletes can enhance muscle strength, optimize energy utilization, and support faster recovery between training sessions, ultimately leading to improved performance on the field or in the gym.

Weight Management and Body Composition: Protein supplements can also be a valuable tool for individuals aiming to manage their weight or improve body composition. High-protein diets have been shown to increase satiety, reduce cravings, and promote the preservation of lean muscle mass during calorie restriction. Protein supplements can serve as a convenient option for those seeking to increase their protein intake without consuming excessive calories or resorting to unhealthy snacks.

Beyond Muscles: Additional Health Benefits: While protein supplements are often associated with muscle-related benefits, their advantages extend beyond physical performance. Adequate protein intake is crucial for optimal immune function, hormone regulation, and the maintenance of healthy skin, hair, and nails. By incorporating protein supplements into their diet, individuals can support overall health and well-being.

Conclusion: Protein supplements have emerged as powerful allies in the pursuit of enhanced performance, be it in the realm of athletics, fitness, or general well-being. Their ability to support muscle growth, aid in recovery, enhance athletic performance, and contribute to weight management makes them a valuable addition to any health-conscious individual's regimen. However, it's important to remember that supplements should complement a well-rounded diet and training program. Consulting with a healthcare professional or registered dietitian can help determine the appropriate type and dosage of protein supplements for individual needs. Unlock the potential of protein supplements and take your performance to new heights!

Buy now: https://energysupplements.co.uk/product/sci-mx-ultra-whey-protein-strawberry-450g If you need more related products then Visit here: https://energysupplements.co.uk/product-category/bcaa-and-intra-workout/   

[randomstudyblr]

(context: I should be getting kinda similar peaks in the blue, red and pink lines. They do not match At All. Ever.)

[mattvaughsworld]

An Overview of Protein Expression and Purification

Proteins are the biological macromolecules maintaining the structural and functional integrity of the body. With many diseases being associated with protein malfunction, there has been a rise in the study of biological function of protein leading to the need of recombinant protein production and purification. Protein Expression and Purification as easy it seems but challenging when it comes to make it perfect for studying the same. Protein purification strategies are existing with an optimal approach toward research and development.

This article would provide a general overview of Protein Expression and Purification:

1. Protein Expression

The best protein purification protocol depends on other factors such as the type of cell used for the expression, the host, and more. Escherichia coli remains the primary choice for most scientists and researchers for producing recombinant human proteins. As they are easier to use, promote rapid cell growth, and are inexpensive. But many of the proteins are tough to be expressed in E.Coli or do not behave the same if produced in E.Coli. Post Translational modifications required for such proteins are different in E.Coli as that of mammalian. Hence many of the proteins which have PTM’s attached to its characteristics are produced in mammalian cell lines or cell lines which support its functionality. One more such system in CHO platform for production of fully functional protein. Many therapeutic proteins are produced in CHO cell line because of glycosylation and other PTM’s.

2. Methods of Protein Purification

The methods available for protein purification are varied starting from the simpler precipitation method to the complex chromatographic arrangements and affinity procedures that are constantly improving with new technological developments. The proteins are purified with existing alternative techniques, yet are primarily characterized by surface features, size, shape, and physical and chemical properties.

3. Protein Isolation

Protein isolation and protein purification is a task that is time-consuming and tedious, thus it has to be efficient and deliver accurate results driving along a suitable technique of choice. There are several kinds of proteins when grouped and numerous when in a cell, making it equally difficult to study every one of them in detail.

Human beings, other living organisms, and higher eukaryotes are complex creatures and with them having systems so vivid, proteins are the vital ones. Protein constitutes the majority of the working system and isolation them is to gain higher insights on their working mechanism and put them in diagnostics and therapeutic usages.

4. Protein Extraction

Protein isolation is to separate protein of interest from the bulk of unwanted substances and other proteins produced by the host. Before we get into extraction, the proteins are both extracellularly and intracellularly produced. The extraction and purification can be designed accordingly  for gaining a successful yield and quality.

For Extracellular Proteins

For extracellular proteins, there is no cell disintegration. The cell culturesupernatant consists of the extracellular proteins which is processed by filtering and downstream processing. The best way to get the protein in its native state is to get it secreted extracellularly, this way there is less of contaminant and hence less challenges in downstream processing. Moreover there is no use of detergent or lysis buffer. For a protein to be produced extracellularly, we need to do protein engineering through molecular biology approach.

For Intracellular Proteins

For intracellular protein isolation and extraction, there is a need for cell disruption, and a suitable lysis buffer is used for the process. The disruption technique must be done considering the protein's physical and chemical properties as they are very sensitive in nature. Sometime detergent is also required which makes the protein denatured. Downstream is more challenging when we use such approach as we need to renature the protein to its original state which is a tedious process.

5. Protein Purification Monitoring

Protein Purification is carried out after every process, and the sample protein is estimated to its level of purity. Through the process, we can know the amount and purity of protein that is obtained.

This process proves efficient while also helps compare different techniques efficiently for protein purification and extraction. There are several procedures for detection of purity levels and monitoring them with effective estimation.

Several protein purification methods are existing in the industry and some of them have been delivering optimal results to serve the whale research and development field with enough resources. GeNext Genomics, a leading biologics such as recombinant human protein supplying company has been in the field of research and development with their expert scientists and professionals.

Conclusion

With taking into consideration the industry concerns, GeNext Genomics develops high-quality biologics such as mAbs, TNF alpha neutralizing antibody, and more. With their protein purification methods have served the research and development industry with accurate results in experiments with respect to recombinant human proteins, and other biologics.

[nenko-dreams]

You consider yourself a protein but you are only a tag.

Great, tag might be useful to purify the protein but it's not necessary for protein-protein interaction.

Or sometimes it can disrupt or prevent the desirable interactions if it is inserted in the wrong place.

But usually it's just there if you couldn't be bothered to remove it or it is not worth wasting your protease.

[hemantallied]

Protein purification is the process of isolating proteins from the complex mixtures of cells, tissues, or organisms based on differences in their physical properties. The protein purification process aims to isolate the huge amount of functional protein with least contaminants.

[inscinstech]

Protein Purification | Inscinstech.com.cn

Unlock the full potential of your proteins with Inscinstech.com.cn advanced protein purification technology. Experience unparalleled results today!

protein purification

[millymiao]

Advantages of protein purification methods

Advantages of protein purification methods

Whether it is protein research or application, it is usually necessary to express the protein in different biological systems (such as E. coli, yeast, insect baculovirus, mammalian cell protein expression system, etc.), and then separate and purify it. Research laboratories usually need to purify micrograms or milligrams of protein, while industry needs to purify thousands of grams or even tons…

[abbkine]

BioAdvisers said on Biotech Advisers

Essential guidance 2 | Fishing with Purekine "fishing gear", Protein Association "wishers hooked"

    In the last issue, the editor brought you “Dry Goods | Using IP “Hook” Fishing, Protein Club “Wishers Take the Bite””, and the response was very enthusiastic. In the voice of everyone, the editor is rushing through the previous experimental notes, bringing you the second bullet of protein interaction knowledge-Pull-Down. If the protein interaction is compared to an interesting “fishing” activity, then the Purekine filler we use is a tough and slender “fishing rod” and “fishing line”. The known GST-labeled recombinant protein is sharp The strong “fish hook” and “fish bait” are the perfect combination, and our target protein is the “fish” that moves freely in the water.

The concept of Pull-Down is similar to Co-IP, and its purpose is to study proteins or ligands that bind to known bait proteins. Pull-Down aims to prove the interaction between two proteins or explore unknown proteins or molecules that can bind to the target protein. Pull-Down is different from IP or Co-IP in that it is not based on antibody-antigen interaction, not an immune response. The bait protein (or ligand) is immobilized on a solid support through a non-antibody affinity system. This immobilization can be covalently coupled to the activated microbeads, or through the receptor molecule on the support The bound affinity tag binds and thus immobilizes. For example, immobilized metal chelating affinity chromatography (IMAC) resin can be used to pull-down histidine tag bait protein. The optimization of Pull-Down needs to consider the special nature of the affinity system used. Principle of Pull-Down According to the following diagram, you can understand the principle of Pull-Down:

Comparison of Pull-Down and protein purification experiments:

Use recombinant gene expression protein, and then perform downstream protein extraction and purification experiments；

Analysis of the similarities and differences between the two experimental procedures；

Experiment type Pull-Down protein purification Solid carrier Affinity chromatography resin Affinity chromatography resin filler Carrier form In bulk Column Incubation method Shaker incubation Incubate in the purification column Type of buffer Binding Buffer

Wash Buffer

Elution Buffer

Binding Buffer

Wash Buffer

Elution Buffer

Target protein form Prey protein Recombinant protein

Types of products to be tested:

Product Category Item No. His tag protein purification tool BMR20000 GST tag protein purification tool BMR20100 MBP tag protein purification tool BMR20206 Flag tag protein purification tool BMR21504 Biotin tag protein purification tool BMR20306 Protein A Antibody purification tools BMR21604 Protein GAntibody purification tools BMR20600 Protein A/G Antibody purification tools BMR20704 Protein L Antibody purification tools BMR20800

Conclusion

The Purkine series of protein purification tools provided by Abbkine are multifunctional and suitable for a variety of protein experiments. When your target protein is a recombinant protein, the purification medium can be used for the extraction of recombinant protein, which is a protein purification experiment; when your target protein is a prey protein that interacts with the recombinant protein, this series of products can be used for prey Extraction and purification.

This issue mainly talks about the knowledge of Pull-Down, time is limited, I have to say goodbye to everyone. At the same time in the next issue, we will have exciting content to meet you all. Stay tuned~~~~~

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the later period. Please continue to pay attention to Abbkine for readers who love science. No public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

[bioadvisers]

Bioadvisers shared on Biotech Advisers

Essential guidance 2 | Fishing with Purekine "fishing gear", Protein Association "wishers hooked"

    In the last issue, the editor brought you “Dry Goods | Using IP “Hook” Fishing, Protein Club “Wishers Take the Bite””, and the response was very enthusiastic. In the voice of everyone, the editor is rushing through the previous experimental notes, bringing you the second bullet of protein interaction knowledge-Pull-Down. If the protein interaction is compared to an interesting “fishing” activity, then the Purekine filler we use is a tough and slender “fishing rod” and “fishing line”. The known GST-labeled recombinant protein is sharp The strong “fish hook” and “fish bait” are the perfect combination, and our target protein is the “fish” that moves freely in the water.

The concept of Pull-Down is similar to Co-IP, and its purpose is to study proteins or ligands that bind to known bait proteins. Pull-Down aims to prove the interaction between two proteins or explore unknown proteins or molecules that can bind to the target protein. Pull-Down is different from IP or Co-IP in that it is not based on antibody-antigen interaction, not an immune response. The bait protein (or ligand) is immobilized on a solid support through a non-antibody affinity system. This immobilization can be covalently coupled to the activated microbeads, or through the receptor molecule on the support The bound affinity tag binds and thus immobilizes. For example, immobilized metal chelating affinity chromatography (IMAC) resin can be used to pull-down histidine tag bait protein. The optimization of Pull-Down needs to consider the special nature of the affinity system used. Principle of Pull-Down According to the following diagram, you can understand the principle of Pull-Down:

Comparison of Pull-Down and protein purification experiments:

Use recombinant gene expression protein, and then perform downstream protein extraction and purification experiments；

Analysis of the similarities and differences between the two experimental procedures；

Experiment type Pull-Down protein purification Solid carrier Affinity chromatography resin Affinity chromatography resin filler Carrier form In bulk Column Incubation method Shaker incubation Incubate in the purification column Type of buffer Binding Buffer

Wash Buffer

Elution Buffer

Binding Buffer

Wash Buffer

Elution Buffer

Target protein form Prey protein Recombinant protein

Types of products to be tested:

Product Category Item No. His tag protein purification tool BMR20000 GST tag protein purification tool BMR20100 MBP tag protein purification tool BMR20206 Flag tag protein purification tool BMR21504 Biotin tag protein purification tool BMR20306 Protein A Antibody purification tools BMR21604 Protein GAntibody purification tools BMR20600 Protein A/G Antibody purification tools BMR20704 Protein L Antibody purification tools BMR20800

Conclusion

The Purkine series of protein purification tools provided by Abbkine are multifunctional and suitable for a variety of protein experiments. When your target protein is a recombinant protein, the purification medium can be used for the extraction of recombinant protein, which is a protein purification experiment; when your target protein is a prey protein that interacts with the recombinant protein, this series of products can be used for prey Extraction and purification.

This issue mainly talks about the knowledge of Pull-Down, time is limited, I have to say goodbye to everyone. At the same time in the next issue, we will have exciting content to meet you all. Stay tuned~~~~~

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the later period. Please continue to pay attention to Abbkine for readers who love science. No public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

[mattvaughsworld]

Learn Effective Methods For Purifying Protein. Contact us or read our blog about protein purification.

[komalghare]

Global Gracilaria Agarose and Gelidium Agarose Market Growing at a CAGR of 5.8% during 2019-2025

Global Gracilaria Agarose and Gelidium Agarose Market Growing at a CAGR of 5.8% during 2019-2025

The report, titled “Global Gracilaria Agarose and Gelidium Agarose Market Professional Survey Report 2019,”studies the Global Gracilaria Agarose and Gelidium Agarose Market across some of the key regions to provide comparative analysis in terms of demand, sales, gross margin and price.

The new research from Global QYResearch on Global Gracilaria Agarose and Gelidium Agarose MarketReport…

[lifesciencepotluck]

Size Exclusion Chromatography

Compared to Reverse-phase, Size Exclusion Chromatography (SEC) or Gel Filtration is a bit more straightforward. As stated in the name it separates proteins based on size (or hydrodynamic volume). Imagine that you want to get the molecular weight of your peptide but there is a butt load of salt which will interfere with mass spectrometry since it relies on the mass: charge ratio.

Gel Filtration is a great for removing salt from your sample. Since you don't use harsh reagents you don't lose the biological activity of your protein. As usual there is a stationary phase and mobile phase. The stationary phase is made of particles or beads with pores of a specific size. The mobile phase is the buffer flowing through the particles and it carries the salts, proteins and everything else in the sample that you apply to the column. The smaller particles (or your tiny salt ions) will flow into the beads and get retained but the larger molecules (the proteins that you actually care about) will flow past the beads and get eluted. It's harder for the column to retain larger proteins so those proteins get eluted at a smaller buffer volume or retention time. The smaller proteins will get eluted with a larger volume of buffer or higher retention time. Since there is a linear relationship between the molecular weight of a protein and the elution volume, so you can use size exclusion to get a rough estimate of protein’s molecular weight.

Figure 1: Chromatogram with elution volumes for proteins of different sizes.  

Figure 2: This is a bead of particle found in a typicla gel filtration column. The bead has tiny pores where small and adorabe solutes (like salt) can actually flow through the resin. Most of the proteins are too big to pass through these tiny pores so they actually flow through the column a lot faster, that’s why you need to less of your buffer to elute larger proteins.

Figure 3: Molecular weight vs Kav (or binding affinity of the protein for column)

A short list of boring but important jargon:

Void volume (V0): This the elution volume of stuff that doesn’t enter the pores or interact with the chromatography resin. These solutes just pass by the particles in the packed bed.

Internal volume (Vi): The volume of the pores within the gel. 

Total volume (Vt): This is the total volume of your column. (It is usually the sum of internal and void volume)

Elution Volume (Ve): The volume of the buffer needed to elute a specific solute. 

Stuff you need to keep in mind

Type of column and pore size: A longer column can give you a better separation of proteins, but it is the improvement in resolution is only proportional to the square root of the column length. Connecting columns with different pore sizes can help separate proteins with a wider range of molecular weights. You have to select your column based on the size of the target protein. Some columns can separate proteins with wide range of sizes (100-10 Kda) others are more suitable for separating smaller proteins (<10 Kda). The uniformity of the pore size is just important as pore size. Generally, if the pore size of the particles is larger you get poorer resolution.

Flow rate: Usually a slower flow rate leads to leads to better resolution or separation of proteins. However, one of the biggest drawbacks of size exclusion is the ridiculously long run time, since the flow rate tends to be slower than other forms like HPLC like Reverse-phase. The typical flow rate is usually between 0.5 ml/min and 1 ml/min. 

Another time-consuming aspect of SEC is the equilibrating the column before use and washing it after use. Sometimes the more hydrophobic proteins end up aggregating and sticking to the particles in the column (like an annoying piece of gum) and you have to use really hydrophobic reagents to wash those proteins out (like Acetonitrile).

Reagents: The buffer you use doesn't affect resolution that much but you want to pick a buffer with low ionic strength and ideal for storing proteins (e.g. ammonium bicarbonate buffer). Usually the beads in the column are silica based so they are a bit hydrophobic and have a weak negative charge.  So, what can happen is that proteins can end up getting retained by the anionic and hydrophobic beads, which gets in the way of eluting and collecting the proteins. Since you don't want any kind of ionic or hydrophobic interactions between the column and the solutes/proteins, adding a bit of salt to the solvent or mobile phase reduces these interactions and makes it easier to elute your proteins.

Another thing to keep in mind is that the shape of the protein affects how it moves through the column. A more rod like protein can move more easily than a more globular protein. BUT we want the column to separate the proteins based soley on size, so a tiny bit of a denaturing reagent (like SDS and guanidine hydrochloride) is added to the buffer. All of the proteins end up with a rod like shape and they are only separated based on size. 

Primary sources

Barth, H. G., Jackson, C. & Boyes, B. E. Size Exclusion Chromatography. Anal. Chem. 66, 595–620 (1994).

Nagy, K. & Vékey, K. Separation methods. Med. Appl. Mass Spectrom. 61–92 (2008). doi:10.1016/B978-044451980-1.50007-0

Recommended readings

GE Healthcare. Size exclusion chromatography: Principles and Methods. GE Heal. Handbooks 139 (2012).

GE Healthcare. Size exclusion chromatography columns and media Selection guide. 1–10 (2016).

[inscinstech]

Protein Purification | Inscinstech.com.cn

Unlock the full potential of your proteins with Inscinstech.com.cn advanced protein purification technology. Experience unparalleled results today!

protein purification

[reporthive-blog]

Global Protein Purification Market Trends and Industry Analysis Report 2018

Global Protein Purification Market Trends and Industry Analysis Report 2018

Protein Purification System Market describing the professional information and in-depth study on the current state of the worldwide. Protein Purification System along with competitive analysis.Protein Purification System exploring the market size, trend, revenue and forecasts up to 2023. This report is a valuable source of guidance for companies and individuals offering Industry Chain Structure,…