#dapis

[lailoken]

"Wouldst Thou Like To Live Deliciously?"

[parasiticmoth]

[dapiando]

the way i was literally just nominated for three oscars. me personally. HUGE DAY

[dont-eat-yellowsnow]

Spheroid of ovarian cancer cells (SKOV-3) stained with DAPI

[starredhowell]

me typing out dapg acronym thinking about how it may not be dapg for much longer… will we still use that acronym to differentiate from dnp as the People. or will we turn to dapy for youtube. it cant be dapc for channel because dapc is crafts. we need to figure this out.

i guess we could do dap for channel and dnp for people but we cant be dapping up the dash lmfaooo. what do u guys think

[libraryspectre]

Artistic rendition of cells under the microscope

Airway epithelial cells transfected with GFP (green fluorescent protein) and RFP (red fluorescent protein) constructs, treated with DAPI nuclear stain

[bloodloss-bloodlust]

dapi

[darkmaga-returns]

A new paper published October 26th discusses NO’s potential in ameliorating SARS-CoV-2/Spike associated inflammation and thrombosis. One from 2005 shows we already knew some of this.

Walter M Chesnut

Nov 22, 2024

DETA-NO inhibits the binding of the recombinant Gamma variant of SARS-CoV-2 spike to A549-ACE2 cells. (A) Cells (5 × 105) were incubated with 2 mM DETA-NO for 15 min, followed by incubation with the indicated concentrations of spike and DETA-NO for another 45 min. The viability was determined by DAPI staining. (B,C) The dose-dependent binding of the Gamma variant of the spike to A549-ACE2 cells was determined by flow cytometry. The results are shown as mean FITC (B) or percentage of binding inhibition (C). (D) Representative histogram plots of the fluorescence of the spike protein bound to the A549-ACE2 cells. The results show the mean ± S.D. of 4 independent experiments (B,C); * p < 0.05 vs. the control condition. ns: not significant. 

As we have discussed many times in this Substack, it would appear that preventing the Spike from entering cells and ameliorating inflammation caused by the SARS-CoV-2 virus are paramount in protecting not only one’s organs but also one’s very own genetic code. To that end, I have continuously searched for natural therapies which may assist us. Nature has yet another weapon to add to our prodigious arsenal against this virus and its malevolent protein: Nitric Oxide. 

First and foremost, Nitric Oxide is a guardian of the Endothelium. 

The vascular endothelium is a monolayer of cells between the vessel lumen and the vascular smooth muscle cells. Nitric oxide (NO) is a soluble gas continuously synthesized from the amino acid L-arginine in endothelial cells by the constitutive calcium-calmodulin-dependent enzyme nitric oxide synthase (NOS). This substance has a wide range of biological properties that maintain vascular homeostasis, including modulation of vascular dilator tone, regulation of local cell growth, and protection of the vessel from injurious consequences of platelets and cells circulating in blood, playing in this way a crucial role in the normal endothelial function. 

[dragonskxn]

Anna is given a thick... GIRTHY.... LONG....FLOPPY.... Leek.

----------

Ratsatsaa ja ripidabi dilla

beritstan dillan dellan doo.

A baribbattaa baribbariiba

ribiribi distan dellan doo.

Ja barillas dillan deia dooa

daba daba daba daba daba duvja vuu.

Baristal dillas dillan duu ba daga

daiga daida duu duu deiga dou.

Hra-tsa-tsa, ia ripi-dapi dilla barits tad dillan deh lando.

Aba rippadta parip parii ba ribi, ribi, ribiriz den teahlando,

La barillaz dillan deiallou ara va reve reve revydyv dyvjavuo

Bariz dah l'llavz dei lando dabaoke dagae gadae due due dei ia do

....What leek?

[lailoken]

I gave Housel to the Horned God of my tradition recently, and afterward, I discovered a pair of enormous Elk tracks nearby that weren't there when I first arrived. Being unknowingly overseen by this particular denizen of the Wild, during this particular rite, felt auspicious enough to me that I retrieved my phone so I could go back and take a photo of the tracks. The fact that there were only two hoofprints that I was able to see anywhere in that area was especially interesting, as the ground was muddy enough to capture all but the softest of treads. It gave the prints a very satyric feel.

[444namesplus]

#209

Pool A

Acam Acane Acbéa Acden Acenn Acfra Acfré Acge Acges Acgil Achel Acis Acjen Acjon Acjul Acloé Acma Acmi Acnie Acode Acpas Acpie Acrah Acste Acsé Alam Alane Albéa Alden Alenn Alfra Alfré Alge Alges Algil Alhel Alis Aljen Aljon Aljul Alloé Alma Almi Alnie Alode Alpas Alpie Alrah Alste Alsé Amam Amane Ambéa Amden Amenn Amfra Amfré Amge Amges Amgil Amhel Amis Amjen Amjon Amjul Amloé Amma Ammi Amnie Amode Ampas Ampie Amrah Amste Amsé Anam Anane Anbéa Anden Anenn Anfra Anfré Ange Anges Angil Anhel Anis Anjen Anjon Anjul Anloé Anma Anmi Annie Anode Anpas Anpie Anrah Anste Ansé

Caam Caane Cabéa Caden Caenn Cafra Cafré Cage Cages Cagil Cahel Cais Cajen Cajon Cajul Caloé Cama Cami Canie Caode Capas Capie Carah Caste Casé

Daam Daane Dabéa Daden Daenn Dafra Dafré Dage Dages Dagil Dahel Dais Dajen Dajon Dajul Daloé Dama Dami Danie Daode Dapas Dapie Darah Daste Dasé Deam Deane Debéa Deden Deenn Defra Defré Dege Deges Degil Dehel Deis Dejen Dejon Dejul Deloé Dema Demi Denie Deode Depas Depie Derah Deste Desé Doam Doane Dobéa Doden Doenn Dofra Dofré Doge Doges Dogil Dohel Dois Dojen Dojon Dojul Doloé Doma Domi Donie Doode Dopas Dopie Dorah Doste Dosé

Elam Elane Elbéa Elden Elenn Elfra Elfré Elge Elges Elgil Elhel Elis Eljen Eljon Eljul Elloé Elma Elmi Elnie Elode Elpas Elpie Elrah Elste Elsé Enam Enane Enbéa Enden Enenn Enfra Enfré Enge Enges Engil Enhel Enis Enjen Enjon Enjul Enloé Enma Enmi Ennie Enode Enpas Enpie Enrah Enste Ensé Eram Erane Erbéa Erden Erenn Erfra Erfré Erge Erges Ergil Erhel Eris Erjen Erjon Erjul Erloé Erma Ermi Ernie Erode Erpas Erpie Errah Erste Ersé Esam Esane Esbéa Esden Esenn Esfra Esfré Esge Esges Esgil Eshel Esis Esjen Esjon Esjul Esloé Esma Esmi Esnie Esode Espas Espie Esrah Esste Essé

Geam Geane Gebéa Geden Geenn Gefra Gefré Gege Geges Gegil Gehel Geis Gejen Gejon Gejul Geloé Gema Gemi Genie Geode Gepas Gepie Gerah Geste Gesé

Hyam Hyane Hybéa Hyden Hyenn Hyfra Hyfré Hyge Hyges Hygil Hyhel Hyis Hyjen Hyjon Hyjul Hyloé Hyma Hymi Hynie Hyode Hypas Hypie Hyrah Hyste Hysé

Kyam Kyane Kybéa Kyden Kyenn Kyfra Kyfré Kyge Kyges Kygil Kyhel Kyis Kyjen Kyjon Kyjul Kyloé Kyma Kymi Kynie Kyode Kypas Kypie Kyrah Kyste Kysé

Maam Maane Mabéa Maden Maenn Mafra Mafré Mage Mages Magil Mahel Mais Majen Majon Majul Maloé Mama Mami Manie Maode Mapas Mapie Marah Maste Masé

Naam Naane Nabéa Naden Naenn Nafra Nafré Nage Nages Nagil Nahel Nais Najen Najon Najul Naloé Nama Nami Nanie Naode Napas Napie Narah Naste Nasé Neam Neane Nebéa Neden Neenn Nefra Nefré Nege Neges Negil Nehel Neis Nejen Nejon Nejul Neloé Nema Nemi Nenie Neode Nepas Nepie Nerah Neste Nesé Noam Noane Nobéa Noden Noenn Nofra Nofré Noge Noges Nogil Nohel Nois Nojen Nojon Nojul Noloé Noma Nomi Nonie Noode Nopas Nopie Norah Noste Nosé

Raam Raane Rabéa Raden Raenn Rafra Rafré Rage Rages Ragil Rahel Rais Rajen Rajon Rajul Raloé Rama Rami Ranie Raode Rapas Rapie Rarah Raste Rasé

Saam Saane Sabéa Saden Saenn Safra Safré Sage Sages Sagil Sahel Sais Sajen Sajon Sajul Saloé Sama Sami Sanie Saode Sapas Sapie Sarah Saste Sasé Syam Syane Sybéa Syden Syenn Syfra Syfré Syge Syges Sygil Syhel Syis Syjen Syjon Syjul Syloé Syma Symi Synie Syode Sypas Sypie Syrah Syste Sysé

Team Teane Tebéa Teden Teenn Tefra Tefré Tege Teges Tegil Tehel Teis Tejen Tejon Tejul Teloé Tema Temi Tenie Teode Tepas Tepie Terah Teste Tesé Tiam Tiane Tibéa Tiden Tienn Tifra Tifré Tige Tiges Tigil Tihel Tiis Tijen Tijon Tijul Tiloé Tima Timi Tinie Tiode Tipas Tipie Tirah Tiste Tisé

Veam Veane Vebéa Veden Veenn Vefra Vefré Vege Veges Vegil Vehel Veis Vejen Vejon Vejul Veloé Vema Vemi Venie Veode Vepas Vepie Verah Veste Vesé

Pool B

Acalch Acar Acart Acbar Accel Acdel Acden Aceg Acelm Aceärn Acfin Acfol Acgil Acgin Acgolë Acgor Achald Achern Acnon Acsnor Acsúri Acth Actin Acval Acéod Alalch Alar Alart Albar Alcel Aldel Alden Aleg Alelm Aleärn Alfin Alfol Algil Algin Algolë Algor Alhald Alhern Alnon Alsnor Alsúri Alth Altin Alval Aléod Amalch Amar Amart Ambar Amcel Amdel Amden Ameg Amelm Ameärn Amfin Amfol Amgil Amgin Amgolë Amgor Amhald Amhern Amnon Amsnor Amsúri Amth Amtin Amval Améod Analch Anar Anart Anbar Ancel Andel Anden Aneg Anelm Aneärn Anfin Anfol Angil Angin Angolë Angor Anhald Anhern Annon Ansnor Ansúri Anth Antin Anval Anéod

Caalch Caar Caart Cabar Cacel Cadel Caden Caeg Caelm Caeärn Cafin Cafol Cagil Cagin Cagolë Cagor Cahald Cahern Canon Casnor Casúri Cath Catin Caval Caéod

Daalch Daar Daart Dabar Dacel Dadel Daden Daeg Daelm Daeärn Dafin Dafol Dagil Dagin Dagolë Dagor Dahald Dahern Danon Dasnor Dasúri Dath Datin Daval Daéod Dealch Dear Deart Debar Decel Dedel Deden Deeg Deelm Deeärn Defin Defol Degil Degin Degolë Degor Dehald Dehern Denon Desnor Desúri Deth Detin Deval Deéod Doalch Doar Doart Dobar Docel Dodel Doden Doeg Doelm Doeärn Dofin Dofol Dogil Dogin Dogolë Dogor Dohald Dohern Donon Dosnor Dosúri Doth Dotin Doval Doéod

Elalch Elar Elart Elbar Elcel Eldel Elden Eleg Elelm Eleärn Elfin Elfol Elgil Elgin Elgolë Elgor Elhald Elhern Elnon Elsnor Elsúri Elth Eltin Elval Eléod Enalch Enar Enart Enbar Encel Endel Enden Eneg Enelm Eneärn Enfin Enfol Engil Engin Engolë Engor Enhald Enhern Ennon Ensnor Ensúri Enth Entin Enval Enéod Eralch Erar Erart Erbar Ercel Erdel Erden Ereg Erelm Ereärn Erfin Erfol Ergil Ergin Ergolë Ergor Erhald Erhern Ernon Ersnor Ersúri Erth Ertin Erval Eréod Esalch Esar Esart Esbar Escel Esdel Esden Eseg Eselm Eseärn Esfin Esfol Esgil Esgin Esgolë Esgor Eshald Eshern Esnon Essnor Essúri Esth Estin Esval Eséod

Gealch Gear Geart Gebar Gecel Gedel Geden Geeg Geelm Geeärn Gefin Gefol Gegil Gegin Gegolë Gegor Gehald Gehern Genon Gesnor Gesúri Geth Getin Geval Geéod

Hyalch Hyar Hyart Hybar Hycel Hydel Hyden Hyeg Hyelm Hyeärn Hyfin Hyfol Hygil Hygin Hygolë Hygor Hyhald Hyhern Hynon Hysnor Hysúri Hyth Hytin Hyval Hyéod Kyalch

Kyar Kyart Kybar Kycel Kydel Kyden Kyeg Kyelm Kyeärn Kyfin Kyfol Kygil Kygin Kygolë Kygor Kyhald Kyhern Kynon Kysnor Kysúri Kyth Kytin Kyval Kyéod

Maalch Maar Maart Mabar Macel Madel Maden Maeg Maelm Maeärn Mafin Mafol Magil Magin Magolë Magor Mahald Mahern Manon Masnor Masúri Math Matin Maval Maéod

Naalch Naar Naart Nabar Nacel Nadel Naden Naeg Naelm Naeärn Nafin Nafol Nagil Nagin Nagolë Nagor Nahald Nahern Nanon Nasnor Nasúri Nath Natin Naval Naéod Nealch Near Neart Nebar Necel Nedel Neden Neeg Neelm Neeärn Nefin Nefol Negil Negin Negolë Negor Nehald Nehern Nenon Nesnor Nesúri Neth Netin Neval Neéod Noalch Noar Noart Nobar Nocel Nodel Noden Noeg Noelm Noeärn Nofin Nofol Nogil Nogin Nogolë Nogor Nohald Nohern Nonon Nosnor Nosúri Noth Notin Noval Noéod

Raalch Raar Raart Rabar Racel Radel Raden Raeg Raelm Raeärn Rafin Rafol Ragil Ragin Ragolë Ragor Rahald Rahern Ranon Rasnor Rasúri Rath Ratin Raval Raéod

Saalch Saar Saart Sabar Sacel Sadel Saden Saeg Saelm Saeärn Safin Safol Sagil Sagin Sagolë Sagor Sahald Sahern Sanon Sasnor Sasúri Sath Satin Saval Saéod Syalch Syar Syart Sybar Sycel Sydel Syden Syeg Syelm Syeärn Syfin Syfol Sygil Sygin Sygolë Sygor Syhald Syhern Synon Sysnor Sysúri Syth Sytin Syval Syéod

Tealch Tear Teart Tebar Tecel Tedel Teden Teeg Teelm Teeärn Tefin Tefol Tegil Tegin Tegolë Tegor Tehald Tehern Tenon Tesnor Tesúri Teth Tetin Teval Teéod Tialch Tiar Tiart Tibar Ticel Tidel Tiden Tieg Tielm Tieärn Tifin Tifol Tigil Tigin Tigolë Tigor Tihald Tihern Tinon Tisnor Tisúri Tith Titin Tival Tiéod

Vealch Vear Veart Vebar Vecel Vedel Veden Veeg Veelm Veeärn Vefin Vefol Vegil Vegin Vegolë Vegor Vehald Vehern Venon Vesnor Vesúri Veth Vetin Veval Veéod

[dapiando]

I was so excited about the bear season 2, i just finished it, it's so cool and awesome and fun and YET i don't get to rb anything interesting about it because there is not a single post that isn't just the most god awful annoying ship discourse I've ever seen in my life. twitter (even TWITTER) has more interesting takes than tumblr rn, all i see here is ship discourse

[postsofbabel]

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[globalresourcesvn]

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[theatretechgal]

To do list when I get back:

Make more formaldehyde

Start processing more tissue

Need specifically some 1cm square pieces, as thin as possible (4mm?) probably RA.

Wanna also do some tiny ones (.3mm square?) goal is to process tiny pieces quickly because they’ll be turned into a hydrogel (I fucking hope)

Make a scuffed freeze-dryer

Slice processed tissues on the cryostat

Make H+E stain and learn how to use it

Stain slices with H+E, separately with DAPI

Learn how to do the H+E stain

Also email chem professor to ask for a pointer to the biochemistry department to ask about membrane protein extraction (someone’s gotta know about that, right?) (might also email the microbio professor at the other uni to do the same thing)

Sign up for a race and power course

Actually file the paperwork for my independent study