#1ml Pipette

[mixelation]

First impression: I can't quite remember if started following you and then read your stories on ao3 or vice versa. I'm pretty certain the first story of yours I read is "do a science." I was delighted to see the organization and operation of a lab be such a key part of the story.

Regarding micropipettes, my second PI made me pipette 1ml of water and record the mass like 100 times a day for a week before I was deemed precise enough to be trusted with the proteins lol.

Now: naruto runs away from my problems (your blog header) makes me giggle every time I see it

your PI made you do WHAT. that's so extra fdhsjkfhjkds

also yessss more lab people!!

[mush-dooms]

big shoutout to the 1ml pipette that litrally fell apart in my hand and shot a piece of itself inside the hood grate :):):) thank god my brain interpreted it as hilarious and not humiliating

[noneedforbloodpressure]

*eppendorf pipettes exactly 1mL of mouthwash into my little paper cup and swishes it around*

[thesuntrapp]

How Many Drops Are in 1 ML?

Understanding how many drops are in 1 ml is important when measuring liquid ingredients or medications. This information can help you avoid making inaccurate measurements, which can lead to dangerous consequences.

One ml of liquid is equal to about 20 drops when using a standard dropper or pipette. However, this can vary depending on the viscosity of the liquid and how precise your measurement is.

Introduction

The ml (milliliter) is a unit of measurement used to measure liquid volume. It’s often abbreviated as ml or mlf, and is the standard unit of measurement for most medical drugs and other liquids. It’s also commonly used in cooking and household recipes, and can be a useful tool when measuring out ingredients.

1 ml is the equivalent to 20 drops, although this number may vary slightly depending on the size of the dropper and how much pressure is applied when dispensing the liquid. This is important information to know if you’re using a dropper, as it allows you to accurately measure your liquids and ensure that you are receiving the correct dosage.

You can use a dropper to easily measure out any liquid, whether it’s medicine, essential oils, or even water for a refreshing drink! With a little bit of practice, you’ll be able to measure out your liquids like a pro. Just remember to keep in mind the 20 drops per ml ratio, and you’ll be sure to get the perfect amount every time!

How many drops in 1 ml?

Having a good understanding of how many drops are in 1 ml can be very useful when measuring out liquid ingredients. It can help to ensure that you are getting accurate measurements and that your final product will turn out the way you expect. This is particularly important when working with medications, IV flush solutions, essential oils and other types of liquids that require exact measurements.

One ml of liquid typically contains about 20 drops. However, this can vary based on the viscosity and surface tension of the liquid being measured. Also, the size of the dropper and how much pressure is applied when delivering the drops can have an impact as well.

In order to accurately measure the number of drops in a particular liquid, you should use a calibrated dropper or syringe. A calibrated dropper will give you a very precise measurement and will allow you to determine the number of drops in 1 ml with great accuracy. This is especially important when working with essential oils as they can have very different viscosities and surface tensions.

How many drops in 1 ml?

Is 1ml The Same As 1 Drop?

A single ml of liquid contains about 20 drops. This is true for most liquids, though it can vary slightly depending on the viscosity of the liquid and the size of the dropper. A larger dropper will typically hold more drops than a smaller one, and the pressure used to apply the drops can also affect their size.

In order to accurately measure 1 ml of liquid, it is important to use a specialized measuring tool. A syringe is often recommended, as it can provide more precise measurements than a standard dropper or pipette. A syringe is also designed to have clear markers for each milliliter increment, making it easier to identify the correct volume of liquid.

A standardized conversion chart or calculator can also help with accurate measurements. These tools will provide a standardized formula for converting milliliters to drops, taking into account all of the factors that can affect the size of a liquid drop. This can be a useful tool for anyone who needs to measure a small amount of liquid, such as when using a medication or dietary supplement.

Is 1ml The Same As 1 Drop?

How Can I Measure 1 ML At Home?

One milliliter is a small unit of measurement that can be useful in many applications. Whether you are measuring liquid ingredients for a recipe or dispensing medicine, understanding how to measure 1 ml can help you make more accurate measurements. This is especially important when dealing with medications or other liquids that require precise measurements.

A common way to measure liquids is by using a dropper. A dropper is a small tube with a rubber container at the end that allows liquid to be dropped out in separate drops. Droppers are often used in medical settings to administer medication or chemical solutions, and they can also be helpful for other applications such as cooking and titration experiments.

To calculate how many drops are in 1 ml, you can use a simple formula. Each drop is roughly 0.05 ml, so 1 ml equals 20 drops. This is a great way to accurately measure liquids without having to purchase expensive laboratory equipment. Just be sure to always use a calibrated dropper or syringe when measuring liquids, as this will ensure that you get the most accurate results possible.

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Are There 20 Drops In 1 ML?

If you’re looking to measure liquids accurately, it’s important to understand how many drops are in 1 ml. This can help you determine the correct dosage of medication or other liquids, as well as calibrate your dropper or pipette for different types of liquids. There are a few factors that can affect how many drops are in 1 ml, including the viscosity of the liquid and the size of the dropper tip.

One way to measure a single ml of liquid is to use a graduated cylinder or syringe. This is a type of measuring device that has a scale on the side and can be used to accurately measure liquids. You can also use a conversion chart or calculator to find out how many drops are in 1 ml.

Another method to measure a single ml of fluid is to use a standard dropper or pipette. Most standard droppers dispense 20 drops per milliliter, so this is a good rule of thumb to use when determining how many drops are in 1 ml. However, it’s important to note that this may not be the case with all standard droppers, so be sure to calibrate your dropper with water before using it with any other liquids.

What Is An Example Of 1 Ml?

As a general rule, 1 ml is equal to approximately 20 drops. However, this can vary depending on the size and type of dropper used. A pipette or eyedropper is ideal for getting precise measurements, but standard droppers and syringes should also work.

The number of drops per milliliter is also affected by the liquid’s viscosity, surface tension, and pressure applied during dispensing. For this reason, it’s important to use a high-quality dropper to ensure accurate measurements.

Having an understanding of how to convert milliliters into other volume units can be extremely helpful for anyone working with liquid medications or dietary supplements. This knowledge can also make it easier to follow recipes that call for specific amounts of liquid ingredients. To help you get started, we’ve created an easy-to-use milliliters to drops conversion table. Simply enter the amount of ml you wish to convert into the table, and it will automatically show you how many drops it is equivalent to. This handy table will save you time and ensure that your measurements are accurate.

What Is An Example Of 1 Ml?

Frequently Asked Question

The number of drops in 1 ml can vary depending on the liquid being measured and its viscosity and surface tension. It can also depend on the size of the dropper or pipette used and the cross sectional area of the opening. This makes it difficult to give a precise answer to this question.

To help you accurately measure your liquids, we've created a handy tool that can convert milliliters into drops in just a few clicks. Simply enter your milliliter value and then select the drop value to see the result.

Drops are an essential part of our daily lives, from taking medication to adding a sprinkle of sugar to your coffee. They can be found in so many different places that it's important to know how to measure them correctly. This is especially true when working with liquid medications and supplements. By understanding how many drops there are in 1 ml, you can be sure that your dosages are accurate and safe. You can also use this information to choose the right dropper or syringe for your needs.

Conclusion

Whether you’re measuring medications, IV flush solutions or essential oils, it’s important to know how many drops there are in 1 ml. This will help you ensure that your measurements are accurate and safe.

The number of drops in a milliliter can vary depending on the liquid’s viscosity, the size of the dropper, and the angle that you hold it at. However, with practice you can learn to measure liquids accurately using a standard eye dropper or pipette.

Measuring liquids accurately takes time and patience, but it’s well worth the effort in the long run. By using a calibrated dropper or pipette, being consistent with the angle of your dropper, counting the drops carefully, and checking your calculations, you can be confident that you’re measuring accurately. In addition, you can also use a conversion chart or calculator to help you measure. Good luck!

[guanshentai]

GST pipette automatic production line-Serological Pipette

　　GST pipette automatic production line Let me talk about the serological pipette first, the English is Serological Pipette, which is a disposable plastic graduated pipette. It was first used as a kind of pipette to replace the glass pipette, and it was called a serological pipette because of its irreplaceable role in aseptic pipetting of serum culture medium. Widely used in tissue culture, bacteriology, clinical, scientific research experiments and other fields that require aseptic operation to transfer liquid.

　　In terms of volume, serological pipettes are mainly divided into six types: 1ml, 2ml, 5ml, 10ml, 25ml, and 50ml. In addition, occasionally 100ml ultra-large volume serological pipettes are circulated in the market.

　　According to the tube type, serological pipettes are divided into stretch tubes and welded tubes. According to the ASTM E934-94 standard, 1ml, 2ml, 5ml, and 10ml can be used as stretched tubes, and 5ml, 10ml, 25ml, and 50ml can be used as welded tubes.

　　In addition, in the industry, in order to distinguish pipettes of different volumes, different color rings/texts are sprayed on the nozzles/wrapping paper for color system identification. Among them, the 1ml pipette has a yellow logo, the 2ml pipette has a green logo, the 5ml pipette has a blue logo, the 10ml pipette has an orange logo, the 25ml pipette has a red logo, and the 50ml pipette has a purple logo .

　　Serological pipette features:

　　The tube head is marked with a prominent color circle logo, which is easy to identify the pipette range model.

　　The scale line is clear and accurate, making it easy to identify the volume of liquid to be pipetted.

　　Bi-directional scale design to meet different liquid pipetting operations.

　　The negative scale design provides considerable extra capacity.

　　The tip of the tube is filled with a filter plug to effectively avoid aerosol contamination of the sample to the pipette.

　　The independent paper-plastic easy-to-tear packaging is convenient for access and provides a medical-grade sterile barrier.

　　Pull-out carton packaging, easy to take out and use.

[hawalab66]

Hawach serological pipettes are sterile. And all sizes of serological pipettes, like 2ml, and 5ml, are made in 100,000 cleanrooms. The serological pipette is also widely made of polystyrene and glass material, which are used to accurately pipette a certain volume of solution.

Hawach serological pipette with stretched tip is mainly made of polystyrene and glass material. And all sizes of the serological pipette, like 1mL, 5mL, and 10mL, are often used for measuring a certain volume of liquid and must be used with a suitable pipette.

[microlitusa]

Variable Volume micropipette  | Best Lab Pipette | Microlit USA

Microlit offers the best variable volume pipettes for accurate and precise sampling and dispensing of the reagent. The micropipette has universal tipcone compatible with all internationally accepted tips. Microlit lab pipette has soft grip with fully autoclavable features. For details, visit the website https://www.microlit.us/product-category/micropipettes/

[not-a-space-alien]

Under the cut are some images I've collected from Google Images that illustrate some of the things in chapter 4 of watch your step, if anyone was unclear on what some of the things in that chapter looked like!!

Dissecting scope--lower magnification than a full microscope, but can still see quite small details

Pipettes

If anyone would like to see a video of how a pipette is used, let me know, I think it's really neat <3

THE INFAMOUS 1ML BEAKER

WHY?????

Mantis nymph

[dogtorari]

Today in lab, we prepared a serial dilution of dirt water collected from our Professors garden! We diluted into each tube from 10^-1 to 10^-8 using our Pipette and 1ml tube.

We then transferred 0.8-0.9U to our Petri dishes and set them in the incubator. I can’t wait to show y’all next class, what it looks like and what bacteria grew!

I hope you enjoy these pictures. These are my lab partners! ❤️🩺

[justarandomgirl98]

I did something to anger the science gods because this is how my day has gone so far:

1. Spilled coffee all over the inside of my car (may not be related to the science gods but it is a notable event.)

2. Missed the bus from the parking lot to my lab due to the spilt coffee. So I was late.

3. Spilt buffer all over myself... it had egg protein in it so I smell vaguely of eggs now.

4. My pipette is calibrated wrong. Didn't realise it until I was trying to figure out why I had so much leftover ELISA reagent... the pipette was suppose to give me 1ml... it was giving me at least 2ml. Meaning all of my antibody dilutions were wrong. So I had to restart.

5. One of my blank wells is coming up positive. Which is weird because there were no antibodies or anything for the enzyme to bind to in the one random well. And my positive controls aren't working...

6. it isn't even 1pm. I am going to go make sacrifices of something to appease the gods so the rest of my plates work. That the positives are positive and the negatives are negative.

[medtechdeclassified]

D. immitis microfilaria on a canine whole blood sample using Knotts concentration technique:

9ml formalin + 1ml whole blood, centrifuge for 5 minutes, decant and add 1 drop of new methylene blue on the sediment. Mix and pipette 1 drop on glass slide, cover with cover slip. Observe under 10x or 40x.

[pennyfynotes]

7.26.18 // 10:00am // things no one ever tells you about research 

so you just landed a position as a "research assistant" huh? whether you're padding your resume, gpa, or you're one of those people who's just super driven and passionate, you might want to read this.. ;)

this isn't super back to school relevant, but i had an epiphany while i was at lab today (waiting for shit as usual) and wanted to make this. this advice is definitely a bit more tailored to biology/medical labs, but def includes some stuff that applies to a bunch of fields! you know the drill, more under the cut! xoxo, m

1. it's not as glamorous as you think it'll be. you're prob thinking “oh yeah i’m mature and i know how the world works. obviously you don’t cure cancer in 3 days.” that is true. (i’m not making fun i actually had a similar mindset). but it will take a *long* time. i’m talking months and years. you might do a a bunch of different experiments and try to get them to work and they never do. i literally worked in a lab for 6 months and didn’t produce a single useful result. it’s also possible it might start feeling like a mindless task or chore. what i’m trying to say is, don’t expect to feel like you’re changing the world. or even that you’re on the road to changing the world. you might have to go through a lot of failure and repetition to get even a little close

2. there’s a lot of waiting. this is definitely more specific to biology/chemistry type labs. but you will be sitting around waiting for reactions. a lot. bring something to do with you.

3. everything takes longer than you think it will. i’m serious. estimate how long something will take if you go slow, take your time, and account for something going wrong. now add at least 15 minutes to that. trust me.

4. you’ll screw up. a lot. you’ll pipette the wrong thing into the tube. you’ll drop glassware. you’ll forget to refrigerate something. maybe you’ll mislabel. don’t beat yourself up too much. be mindful and learn from mistakes, but know that everyone makes them. pi’s (principal investigators aka your boss/the researcher you work for) are generally pretty forgiving, especially in the first bit.

5. don’t mix bleach with anything but water. please don’t do it. take it from someone who has. it was terrifying.

6. you need a schedule. i don’t mean that you have hours and work 9-5. both of the labs i’ve worked in couldn’t care less when i came in. however, you need to plan things. if you’re working with cells, they’re a pain in the ass. once you get familiar with them, you’ll need to map out what/when you want to run experiments so you can make sure they’re ready at that time. or if you need to work with someone else to run experiments. for example, i work with a technician for certain assays and need to make appointments with her. or some processes are time sensitive and once you start them, you can’t stop until they’re over. don’t try to cram too much into one day or you’ll never go home. 

7. weekends aren’t really a thing. ok this depends on how you structure your time/if things cooperate (hint: they never do)/what sort of research you’re doing. but i go into lab at least once every weekend pretty much. honestly having the whole lab to myself is kinda relaxing lol

8. bring headphones. otherwise you might go crazy from not being able to talk to anyone. also they can help you concentrate.

9. go slow. i mean this advice comes up in relation to everything. but it is *so important* for lab work. if you’re rushed or nervous or both, you’re way more prone to making mistakes. and lab reagents are expensive as all hell. seriously. look up the price of some sybr green. 

10. ask questions. the other people in your lab/shared lab space are great resources. generally they’re pretty nice. obviously, senior members know a lot more than you do. but also other undergrads. someone else might be an expert in something you’re just learning. it’ll make sure you don’t break/waste anything and give you peace of mind. at the very least, you can share some accountability if you screw up lol.

11. check everything. if you’ve done an experiment a bunch of times and it still doesn’t work even though you fixed everything you thought you were doing wrong and asked a bunch of questions, you’re probably missing something. ideally, have someone watch you do the process. if not, go over your protocol with them. every single step. and every detail. i literally thought i was doing something wrong for months because i was using human dna primers and not mouse ones. i had checked everything else, but it never occurred to me to verify that. 

12. document everything. as you learn, you’ll get more familiar and figure out what works best. you remember it now, but if you haven’t done something in 3 weeks and need to remember that one trick you figured out, you’ll be kicking yourself if you don’t write it down.

>> similar to documenting, label everything. know *exactly* what’s in a bottle because you’ll remember 3 days after you made it but in 3 months? might land you in trouble.

lightning round! mostly bio/chem/wet lab stuff!

- check the volume your micropipette is set to uptake. just because you grabbed the p1000 doesn’t mean it will automatically be sucking up 1mL

- accidentally sucked liquid up into the body of your automatic pipette? now it doesn’t work? you didn’t break the whole thing, it just needs a new filter. they’re about $5.

- always label your damn tubes. before you put stuff in them.

- a quick spin in the centrifuge is your best friend. especially if there was supposed to be 5 uL in that tube but you’re only getting 3.5

- put your agarose gel in the fridge until really hard before removing the comb. also remove the comb once the gel is submerged in buffer.

- it’s ok to use your other hand to brace the pipette if you’re shaky. it’ll save you a lot of grief.

- use old pipette boxes as racks for smaller tubes

- cells wait for nothing. not even new year’s day. 

- use a timer. i’ve forgotten about things so many times, especially when i’m multitasking

- make note of any prep work that needs to be done before an experiment. (ex: set the centrifuge to 4 degrees) now you’ll know to do it beforehand and save yourself time.

- make sure equipment is available. before starting anything, make sure the stuff you’ll need to use will be ready when you need it. put a post it on the machine if you have to.

- keep a jacket in lab. i guarantee it will be cold. if you work in lab during the school year, you might want to keep some closed toed shoes and long pants there too depending on the “dress code”.

- learn basic lab maintenance/where stuff is asap. it sucks to be the only one in lab when you run out of something and don’t know how to fix that.

hope this was useful and let me know if you want to know more on this subject! or on anything. you know i love suggestions. xoxo, m

[juniperpublishers-ecoa-blog]

Juniper Publishers - Open Access Journal of Ecology

First Record Fungi for Iraq

Authored by : Hussein Al-Nasrawi

Abstract 44 fungal species were isolated from plant parts submerged in Al-Huwaiza marsh within Iraqi borders, and 7 new first records fungi in Iraq were isolated too, which have been illustrated and described as follows: - Carbosphaerella leptosphaeriodes, Curvularia lunata var.aria, Graphium sp., Helicascus kanaloanus, Leptosphaeria obions, Stagnospora sp. and Ulocladium tuberculatum, Carbosphaerella leptosphaeriodes, Curvularia lunata var.aeria, Graphium sp., Helicascus kanaloanus, Leptosphaeria obions, Stagnospora sp., Ulocladium tuberculatum.

Keywords: Fungi; Submerged plants; Marsh; New record; Iraq

Introduction Al-Huwaizah marsh is an aquatic ecosystem extend between Iraq and Iran with freshwater body.Al-Huwaizah marsh locates between latitudes 31˚45ˉ and 31˚00ˉ in the north and longitude 47˚50ˉ and 47˚ 25ˉ in the east , passing through Iranian borders , 80km X 30km [1]. Reed plants (Phragmites Australis Trin) and Typha (Typha Australis Schum & Thonn) are the main components of the vegetative cover in the marsh ecosystem [2]. Many endemic fungal species play an important role in the biodegradation and bioremediation of marsh environment. Fungi play an important role in biodegradation process of plant debris submerged in marsh and bioremediation occurs during mycoremediation during decomposers (fungi) in the aquatic environment along with some types of bacteria [3-5]. Hussein Al-Nasrawi (2006) was confirmed isolation of fungal diversity (fifteen species) as a new record for the first time in Iraq, isolated from the plant remains submerged in aquatic ecosystems in Iraq , in addition to many studies conducted in Iraq for the same mycological puroses [6,7]. Many species of Basidiomycetes were isolated from stems and leaves of the reed plant submerged in salt marshes in Belgium [8].

Materials and Methods Collection of samples 50 pieces of decomposed plants were collected from water body and sediments in Al Huwaizah marsh in Iraq during 2016. Samples were washed gently by tap water and then by distilled water. Plant debris were cut into small parts 7-5cm long and each 10 pieces were settled in the bottom of petri dish.

Preparation of culture media Potato Carrot Agar (PCA), which was obtained by weighing 20g of potato and carrots after washing and peeling, then sliced and boiled with a quantity of distilled water, was sprayed well in a ceramic vase , filtered and placed in a 1 liter flask then added to the prepared mixture of each of the potatoes and carrots media .the media objected to sterilizing process in autoclave under standard conditions for 20 minutes (250mg of chloramphenicol as antibiotic to inhibit bacterial growth.

Insolation and identification of fungi In this study, two methods were used to isolate the fungi: direct isolation from the substrate. The humid chamber method was used to remove the previously prepared vegetable pieces from the beaker using sterile forceps and placed 7 to 5 pieces in a glass bowl of 15cm diameter Petri dishes Sterilize the filter leaves, then moisten the filter leaves with sterilized distilled water and incubate the dishes under 25°C. The second method is the method of dilution. Dilution method to isolate the fungus from the washing of submerged plant parts and summarized the method by withdrawing 10ml of sterile distilled water, which was washed by the samples previously using a sterile pipette placed in a flask containing 90ml of distilled water and a well and withdraw from it 1ml transferred to A sterile glass dish with a diameter of 9cm. The food medium, plate roast and incubation were incubated under 25°C. Three replicates were made of each sample. The isolated fungi were classified under light microscope by using international taxonomic keys published in the following literatures: [9-20]

Ascocarp 90-120um in diameter, globose to subglobose shape. The Asci 40-45 × 60-80μm with 8 Ascospores. The Ascospore 15- 18 × 25-30μm, devided by triseptate, the two mid cells within the ascospore dark to brown color, whereas the terminal cells pale and surrounded by mucous sheath. The present isolate nearly like the isolate of Schmidt [21]. This species considers as a new record for Iraq. The isolate was illustrated and kept in under no. BASRA 2011 (Figure 1).

Curvularia lunata var.aeria (Batista, Lima & Vasconselos) M.B.Ellis.1960, publcos inst Micol Recife 263: 5-10

The colony with black to gray color, the hyphae immersed under substrate surface. Conidiophore thicker than fungal filament (macronematous), subhyaline. Conidiogenous cell is polytretic. The conidia with curve shape divided with three septae to form four cells, the two mid cells thicker and darker from the two terminal pale cells. Conidia 10-15 × 20-30μm. This species was previously isolated from painted wood and soil whereas our present fungus isolated from reed sample submerged in marsh sediments. Dry culture was kept in Basra herbarium under no. BASRA 2012 (Figure 2).

The colony is gray to Olivaceous brown, Conidiophore thicker than fungal filament (macronematous) appears under dissecting microscope as Synnemata. Fungal hyphae immersed under the epidermis. Conidiogenous cell is monobasic type. Conidia 5-7 × 15-20μm. Oval to cylindrical shape, with rounded end, pale color without, unseptated. Our isolated fungus resembles species Graphium putredinis isolated by Huges [19]. Our present isolate differentiated by its shape and size (cylindrical 2-4 × 5-11μm. The species isolated from reed segment submerged in marsh sediment. Dry culture was kept in Basra herbarium under no. BASRA 2013 (Figure 3).

The Ascocarp globose, immersed, 400-250μm high, 400- 800 with ostule. Black to dark brown color. Asci 200-300μm., bitunicate, with 8 ascospores. The ascospore 15-25 × 35-50μm. Arranged inside ascus as uninervate. The ascospore divided by septum into 2 dark cells, with funnel shape. cell wall of ascospore surrounded by two layers. There are two germination pores in the ends of ascospore. The ascospore differentiated by gelatinous layer clearly appears when immerse in water drop (disappear with lactophenol stain). The present fungus isolated from Typha segment submerged in marsh sediments, illustrated and kept in Basra herbarium under no. BASRA 2014 (Figure 4).

Leptosphaeria obions (Crouan et Crouan) Saccardo Syll Fung 2,24,1883.

Ascocarp sub globose, immersed, with high about 100-300 and diameter 200-400μm., black to dark brown color, usually covered by brown filaments. The ascocarp coated by two layers, large dark external layer and pale small internal layer. The asci thick, bitunicate,14-20 × 150-300μm. Each ascus contains 8 ascospores, 8-15 × 25-40μm. Arranged inside the ascus as uniseriate in the top of the ascus whereas as biseriate in middle site. The ascospore divided by three septae to form 4 cells, the two middle cells dark brown and larger than the terminal smallest cells. This species was previously isolated from herbal plants and from mangrove area in Australia. The present fungus isolated from Typha segment submerged in marsh sediment, illustrated and kept in Basra herbarium under no. BASRA 2015 (Figure 5).

Stagnospora sp

The Pycnidium sub globose, partially immersed, with pale brown ostiole and short papillate. High of pycnidium 150-180μm, 100-200μm diameter with a neck about 10μm diameter. The conidia pale to brown color, cylindrical in shape, 4-8 × 40-70μm., divided by 5-7 septae. The present isolate resembles Stagonospora haliclysta which was previously isolated by Kohlm [22] (conidia size 3.5-4.5 × 20-27μm, smaller than our isolate). The present fungus isolated from reed segment submerged in marsh sediment and consider as first record in Iraq. It was illustrated and kept in Basra herbarium under no. BASRA 2016 (Figure 6).

Ulocladium tuberculatum Simmons, 1967, Mycologia 59: 83 -84.

The fungal hyphae immersed, sub hyaline, with thick conidiophore 4-6μm. 160-200μm. length, pale brown color, divided by septae. Conidia 10-16 × 10-20μm. sub globose, like potato fruit, divided into several parts by septae cross shape.

The species was firstly isolated in united states. The present isolate resembles Tetracoccosporium paxianum, which isolated by Szabo,1905. Our species was isolated from reed segment submerged in marsh sediment, kept in Basra herbarium under no. BASRA 2017 (Figure 7).

 Discussion

Fungi Inhabit plant segments submerged in aquatic ecosystems used their enzymic complex system to biodegrade cellulose and produce carbon source, the most important matter for fungal metabolism process [23,24].

Guaro et al. [25] & Guaro et al. [26] the pioneers who worked on wetland area in Iraq, they isolated and identified many new fungal species and new record fungi forom plant segments submerged in marsh ecosystem in southern area of Iraq. The present study choosed one fresh and natural premium deep marsh ecosystem called al-Audem in Mysan province to suray fungal diversity and new records .This ecosystem consider a natural , undiscovered mycoflora enriched with organic materials and with high quality sediments settle in the bottom of water body , that encourage growth of different fungal species. The present study contributed in recording seven new record fungi for Iraq from this marsh environment [27-34].

Conclusion

Several marshed in southern area of Iraq still waiting more studies and novel works to discover more new species and new record fungi. The high-quality water parameters of marsh ecosystem with enrichment of plant diversity, leads to establishment a perfect foundation of sediment layers embedded in the bottom of marsh environment. This study opens the track for researchers to investigate the ecological niche of fungi in marsh community to detect more aquatic and sediment mycoflora of wetlands.

[sadklwd]

Gilson 25ml Serological Pipette

The Gilson 25ml Serological Pipette is the perfect instrument for daily use in the cell culture, molecular biology, and microbiological fields. Its high clarity polystyrene construction, red colour-coding, and premium material barrier plug provide superior transparency and durability. These pipettes also comply with the strict standards of the ASTM Standard Specification for Serological Pipettes E-934.

The polystyrene pipettes come individually wrapped in paper-backed plastic wrappers. They feature large,  Serological Pipette  clear volume indicators and a graduated system with descending and ascending numbers. Additionally, they are color-coded according to volume. These pipettes are manufactured in an ISO-9001:9002-certified facility in the USA. They are non-cytotoxic, RNase/DNase-free, and pyrogenic-free.

The plastic serological pipettes produced by Globe Scientific meet stringent manufacturing protocols and provide high accuracy. They are also supplied with an aerosol barrier plug, which helps prevent any liquid or aerosol contamination. These pipettes are also available in 1mL, 2mL, five-mL, and 10mL sizes, as well as a 50-ml size for larger samples. It is important to choose a pipette that works for you, and the right one can help you achieve success in your lab.

[scontomio]

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