#aa batteries dna

[knights-of-the-glittery-table]

Good morning, here is a free sample of being mentally ill and on your own, no cost at all.

Last Wednesday the fire alarm downstairs went off for 4/5 ear splintering rounds at 1:30am then fell silent as if nothing happened. Definitely no fire/smoke/heat issues. I put it down to a fluke.

Half an hour ago it did The Beep right above my head. You know The Beep. That sound that means ‘change my batteries’ but mine are connected to the mains so I was like ‘oh.’

I used the website I used to find the plumber and asked for a callback but like...it did the chittery beep twice again at intervals. I knew it’d be like the beating of the telltale heart going off every once in a while

So I googled it.

Turns out likelihood is, the 9 volt battery used as a backup could need replacing.

I had ONE nine volt battery by pure fluke. I have a little box filled with batteries (all new) and they are you know, the usuals, AA’s and then some of the tiny round bastards. BUT THERE WAS ONE 9 VOLT. FOR REASONS I DON’T RECALL.

So I get out the stepladder (may I bring up to you at this point I have fallen arches that mean I am very very nervous of being even up on step stools due to 0 balance ability and 1000 incidents to go against faith in my ability) and I go to twist it off.

Nope.

Get back down.

Google how to open a bloody mains fire alarm.

First one is too old. Talks about a pin to pull out. No bloody pin to pull out.

Climb back down, check anothe youtube video.

I HAVE TO PUT THE HEAD OF A FLAT SCREWDRIVER INTO A SLOT AND THEN IT JUST COMES OFF.

It did.

I’m shaking like a leaf due to hand tremors I’ve had since March due to heightened anxiety that just never left and worry I will fuck this up.

Climb down. Use teeny tiny mini screwdriver to get the screw out of the plastic, see a 9 volt battery in there. It hasn’t gone off or yelled at me yet.

It’s difficult as hell to get the battery out even though there’s a little ribbon tab, you know the type you pull on and typically the battery pops up and is easy to remove?

Fight with the thing. Finally get it out. Have to unattach it from the wires. One was easy, the other was NOT.

Fetch new battery from the stair I’d put it on. Attach it to connecting things and then realise the electrician who worked on it last PUT THE FUCKING BATTERY IN THE WRONG WAY, THAT’S WHY IT WAS SO HARD TO GET OUT. Put it back in the OTHER WAY this time and components and wires fit much easier.

Tighten the tiny screw on the battery compartment.

Stick it back up and twist so it’s back on the ceiling.

Stand utterly anxious to the strands of my DNA in case it suddenly goes off or returns to its chirps.

Nothing. Did I do it?

Literally stood under the fire alarm and said aloud to utterly no-one

DID I DO IT?!

Sit down a moment, hands are shaking like I’m sitting through a high level earthquake and casting glances of supreme concern to alarm.

....It has not chirped at me again. It hasn’t gone off and scared me to death either.

Did I do it??

I might have done it!

[sycriptouk]

FDA Gives Inovio Green Light for Phase 3 Trial of DNA COVID-19 Vaccine ~ November 11, 2021 🧬🧬🧬🌎🌎🌎

Inovio ($ino) has received the FDA go-ahead for a phase 3 trial of INO-4800, a DNA plasmid vaccine against COVID-19.

The agency lifted a prior clinical hold on the study after Inovio satisfied questions about the vaccine and its needleless electroporation delivery system, Inovio’s Joseph Kim said on an investor webcast.

Inovio and partner Advaccine Biopharmaceuticals Suzhou Co. (China) will conduct the INNOVATE study in Mexico, Brazil, Columbia and Thailand, in addition to the U.S. The trial will evaluate INO-4800 in healthy adults aged 18 years and older, who will get two 2-mg doses given one month apart. The primary endpoint is virologically confirmed symptomatic COVID-19.

“We are aiming to have the interim phase 3 data available by the first half of 2022,” after which Inovio will apply for an Emergency Use Authorization, Kim said.

This is the first large-scale test of a DNA plasmid vaccine for COVID-19. DNA vaccines have some unique advantages over mRNA vaccines, including a relative insensitivity to temperature. The vaccines have shown 1-year stability at temperatures of up to 98.6 degrees and have a projected 5-year shelf life at normal refrigeration temperatures.

The company’s proprietary delivery device is a key component of the vaccine system. The hand-held unit, powered by AA batteries, uses air pressure to force the vaccine into the epidermis. It then generates millisecond-duration electrical pulses that temporarily open pores in all cells lying within the electrical field, including immune cells. This allows the plasmid ring, encoded with the viral antigen, to enter easily. Electroporation increases the amount of DNA delivery by more than 1,000-fold compared to traditional injection delivery, the company said.

After uptake, immune cells use the plasmid DNA sequence to generate antigens; this stimulates an immediate immune response as well as the long-lasting humeral immune memory vaccines rely on. DNA vaccines do not interfere with or change in any way an individual’s own DNA, the company added.

“The CELLECTRA 3PSP device is designed specifically for a COVID-19 type pandemic scenario,” the company said in a press statement. The user-friendly design “allows for stockpiling of the device in quantity without maintenance … [It] has been designed with reliability, challenging environments, user needs and ease of large-scale manufacturing in mind.”

Development of this device really took off in 2019, with an $8.1 million investment from the medical arm of the U.S. Defense Threat Reduction Agency’s Medical Chemical, Biological, Radiological and Nuclear Defense Consortium.

The lifting of FDA’s clinical hold is another in a series of green lights flashing for Inovio, Kim said on the webcast.

“The World Health Organization shared on Oct. 26 that our candidate is one of two that will be trialed internationally in the Solidarity Vaccines study. We believe INO-4800 is well-positioned to combat this global pandemic.” — Michele G. Sullivan

submitted by /u/WallStBull10Point0 [link] [comments] from wallstreetbets https://www.reddit.com/r/wallstreetbets/comments/qudgks/fda_gives_inovio_green_light_for_phase_3_trial_of/ via IFTTT

[swldx]

Radio New Zealand Int. 1321 18 Sep 2021

5980Khz 1259 18 SEP 2021 - RNZ NATIONAL (NEW ZEALAND) in ENGLISH from RANGITAIKI. SINPO = 45333. English, bellbird int. until pips and news @1300z anchored by Vicki McKay. Police are still working through DNA evidence to identify the mother of a baby girl whose body was found at an Auckland recycling facility a month ago, but say that caring gestures from the community have been touching. Community members had gave the baby the name Anahera, which means angel, and provided a kahu huruhuru cloak for her to wear at her eventual funeral. Covid19: Thousands of police deployed to stop Australian anti-lockdown protests. Haiti’s top prosecutor fired by PM after trying to get him charged for president’s assassination. A woman accusing Prince Andrew of hiding from her sexual assault lawsuit can try an alternative means of serving her legal papers so he can address her claims, a US judge has ruled. Sports. Pele in and out of Sao Paolo hospital. @1304z trailer for RNZ National "Tomorrow Night". @1305z Weather forecast: mainly fine with some showers. @1306z "All night programme" anchored and DJ'd by Vicki Mckay. MLA 30 amplified loop (powered w/8 AA rechargeable batteries ~10.8vdc), Etón e1XM. 100kW, beamAz 35°, bearing 240°. Received at Plymouth, United States, 12912KM from transmitter at Rangitaiki. Local time: 0759.

[onlineblogsworld]

The Best Way to Purchase a Plug and Play Vaporizer

Not long ago, when a brand-new vaporizer was released called the Plug and Play Vaporizer, it appeared like vaporizer fanatics would line up to have a chance to experience this revolutionary product. It had been designed by two men named Eric Ries and Colin Stuart, who had designed the first vaporizers back in 1995. They needed to come up with a new product when they couldn't create vaporizers which were strong enough for professional vapers Cannabis edibles.

The Plug and Play Vaporizer guarantees a simple vaporizer experience with prefilled pod cartridges. Not only does it provide a superior battery which outshines many different vaporizers. However, their extraction specialists are dedicated to crafting high quality centers using the highest quality materials. Twist is their best of the lineup vaporizer pencil offered in their current DNA and Exotic series. And finally, play is their cheap rechargeable battery-powered vaporizer that makes availability of top quality flavors easy.

The greatest selling point of this Plug and Play Vaporizer are you do not need to use a gas supply like a cigarette. They will not force you to feel drowsy. Although it's extremely mild, the digital components inside work really well. That is because they use a lithium-ion rechargeable battery, which has the exact same power and efficiency as your normal AA batteries found on your normal vaporizer. The vaporizer will even run quietly while working on your most convenient temperature range.

A lot of men and women who buy plugs and perform vapors are in fact quite serious about using them. They buy them because they want to enjoy the amazing adventure of smoking cannabis. However, cannabis oil isn't available everywhere. You may only legally buy plugs and perform kits from licensed online sellers. However, many men and women find this out by accident and wind up buying cheap cannabis oils online that they think are real.

To avoid getting scammed, always make sure you buy your vaporizer from accredited online vendors. If it sounds too good to be true, it probably is. Many times there are middlemen involved with online transactions, and it is easy to fall for them. Always confirm the company's credentials prior to purchasing your vaporizers and accessories Marijuana for sale. As soon as you locate a trusted seller, then you need to order everything from the same company.

If you want to try out a Plug and Play Vaporizer, you must first check online shops like Vapor Depot. There are typically several options available, and each includes a free 3-d digital thermometer. As soon as you obtain your kit, then you can always send in your finished heating unit to get a refund or exchange it for a different model. There are a variety of selections to pick from, which means you are not locked into only 1 style. You can even buy additional accessories, such as, for instance, a glass bulb or a drip tip adapter if you would rather get an aromatherapy experience. With a few clicks of your mouse, then you can now take advantage of the Plug and Play VW Cartridge and get ready to enjoy high quality cannabis infused vapor anytime.

[lazuliquetzal]

someone left a comment on aab like "I know it's been a few years since you wrote this" and what. the passage of time

[okbuyok001]

Automatic Fly Trap

Automatic Fly Trap

Flies aren't only annoying—they're also extremely dangerous! They carry bacteria and diseases that can contaminate your food. Automatic Fly Trap keeps flies and pests away from your table so you can relax and enjoy outdoor meals. It has holographic dots on the blades so you aren’t constantly swatting bees, wasps, or flies while you’re camping. No more nasty, disgusting bugs crawling all over your sweet treats! Features: SOFT-STOP BLADES, CORD-FREE: Blades come to a stop if something touches them, then resume as soon as the obstacle is removed. WHAT KEEPS THE BUGS AWAY? Dots refract/bend light which scares the insects. Bending light is not a part of their DNA. In their little fly minds, the bending colored lights are not normal in nature and it appears dangerous to them. They will always choose to go somewhere else. KEEPS FLIES OFF FOOD: You can finally enjoy a conversation over an outdoor meal without swatting flies all the time. This is a light-weight, odor-free fan you place on top of your outdoor or picnic table to keep flies from dive-bombing your food. ENJOY THE OUTDOORS: Sunbathe, play cards, sit by the pool or dine out on the back deck without kamikaze flies landing on you or hovering over your food. Order your ShooAway now and you will be surprised how well it works. Specifications: Power: Two AA batteries(Not contain batteries) Size: 1.4 x 1.4 x 3.9 inches Color: Black Packing Includes: 1 x Automatic Fly Trap  

Read the full article

[cyuya-nakahara]

“I’m Playing Yugioh!”

read on ao3

Wherein Dazai's pestering Chuuya, Chuuya wants to beat Kajii's insufferable deck once and for all, and Kajii just wants to see Chuuya smile.

Happy Birthday, @ch-ch-ch-chuuya!! Playing competitive ygo has finally paid off....by giving me the knowledge needed to write this fic. I hope you like it!

"And next, I'll—" "Ugh, hold on, Dazai's texting me." Chuuya said, hearing a distinct tone ding from his phone. Kajii put the card he'd been about to play back in his hand. "What does he have to say?" He asked, genuinely interested. Chuuya shrugged as he dug out his phone. "I don't know but whatever he wants it's not as important as me kicking your ass here in a minute." "Pfft, you wish, but do send me a postcard from the Shadow Realm." Chuuya grunted in irritation. "I mean it when I say I've about had it with that deck of yours." Kajii had been beating him with a bunch of batteries every time they played for weeks. He'd tried so many decks but this time he was going to win. Like...for real, this time. And with the lay of the field right now... Kajii had three Batteryman AA and a Charger out now, and Chuuya had four monsters of his own. He'd have to tribute one for his win conditions, but he could win. Chuuya swiped open his phone. "'Hey babe I'm horny.'" he read aloud with a deadpan expression. He scoffed. "Of course that's what he has to say." He huffed to himself and typed back. Kajii chuckled. "I'm playing yugioh," he sent, and set his phone aside. "Go ahead." "Where is he anyway? He hasn't been by since left the house this morning. Do you think he's trying to sext you from the grocery store again?" "Just go, I'm ready to kick your Batteryman ass." Kajii grinned and obliged, taking a card from his hand and raising it dramatically. "Well, since I have three or more Batterymen on the field, I'll activate—" Chuuya waved his hand. “Oh no, you've had three Batterymen for the past 2 turns, and you've been playing what you draw, have you just been hanging onto that card just for the hell of it?" Kajii practically glowed as he set Short Circuit onto to field. "I drew it in my starting hand, my dear lemondrop; it's much more fun when your field has more monsters. Can you counter it?" "No, but do you realise you don't have to play like a jackass?" "I like to watch your reactions. Each  of your defeats is a valuable sample." Chuuya gave him a look and put his four monsters in the graveyard. Kajii moved to continue his turn and go in for the kill. "Now I'm going to—" Chuuya laughed. "Attack directly for 9000? Yeah, no; I activate Threatening Roar." "Then I pay 1000 Life Points to activate 7 Tools to negate it!" Kajii said, dramatically flipping a trap of his own. But Chuuya was ready. "Then bitch, so do I!" He said with a grin, flipping up a second trap card triumphantly. Kajii frowned. He wouldn't be able to attack that turn after all. Not that he was particularly worried about it. It'd be a little more difficult, and he wouldn't get the really fun OTK, but he was still set up well and Chuuya's new Machine deck was only sort-of holding together. It wasn't anything flashy. "Fine." He conceded. "I end my turn. We both pay 1000; I have 5250 and you're at 4500." Chuuya's phone went off again. "BITCH! I'm playing Yugioh!" He said loudly at the screen, as he read Dazai's message, his nails audibly tapping around as he typed his response. Kajii smiled softly. Chuuya was so...expressive. "Tell him to come over." Kajii suggested. But Chuuya was already drawing a card from his deck to start his turn. "I told him to just jack off." The redhead said lightheartedly. A smirk grew on his face. "Though he really should be here to watch me finally hand your ass to you." "Ooh, cocky banter, is this where I'm supposed to be concerned?" "Tch." Chuuya placed a card on the field, and then another. "Since I don't control a monster, and you do, I can special summon Cyber Dragon from my hand. Then, for my normal summon, I'll play Biofalcon." Kajii pretended to not be listening. "Did you know that you can make batteries from lemons? I can show you after I use these batteries to win again." "Oh, shut the fuck up, I activate DNA Surgery and—" Suddenly Kajii understood the purpose of Chuuya's deck. "Oh no, you won't, I use 7 Tools—" Kajii stopped mid-sentence and looked down at his Spell and Trap Zone, cursing under his breath. He'd used it already. Chuuya's grin stretched across his entire face. "Can't negate?" He asked with a smirk. Kajii just looked at him with dread, and Chuuya continued. "In that case, I activate DNA Surgery and I call Machine. All monsters on the field are now Machine monsters." Kajii's ego was dropping rapidly and was fast on its way to the molten core of the Earth. "You have one of those don't you?" He said with resignation, watching Chuuya's face. The redhead looked on top of the world as he pulled a card from his Extra Deck. "If by 'one of those' you mean a Chimeratech Fortress Dragon, fused from every monster on the field, that's attacking you directly for 6000, and therefore for game? Yeah, I have one." Kajii clapped slowly. "You win, good job, babe." Chuuya smiled happily as he picked up his cards and shuffled his deck. He was really cute, and Kajii wondered how many successive victories it would take for his reactions to shift. He picked up his own deck and put it away. "Finally." He said, collapsing backward on the bed. Kajii picked up his cards and laid down beside him, playing with a strand of his hair. "That was a great play." He praised.   Chuuya chuckled. "Someone had to do away with those batteries." "Then I'll just have to find a new build to test you with. The pursuit of science never ends."   "What the hell sort of science are you doing anyway?" Kajii chuckled, enjoying the idle comfort of lying with Chuuya, of his hair between his fingers and his small frame beside his. He didn't answer Chuuya's question. The results would be tainted if Chuuya knew the details of the study. Kajii looked down at Chuuya's face. A satisfied smile was still present on his lips. His eyes were closed, and he looked comfortable and happy. A knock came from the door. "Can you get that, Motojirou?" Chuuya drawled. Kajii nodded and kissed his forehead softly before pulling himself off the bed and making his way to the door of the apartment. When he opened the door, he found Dazai standing outside, a large pizza and tray of cookies in his hands, and a grocery bag hanging on his arm. "Hello, Motojirou!" He said, his voice practically singing. A kind smile was on his face. "Did Chuuya win yet? I brought things to celebrate." Kajii smiled, opening the door wider for Dazai to get in. "He won about ten minutes ago," Kajii said as Dazai came in and started toward the bedroom, Kajii following behind him. "How'd you know he'd win?" Dazai's foresight actually regularly amazed him. He was nightmare to play with, he was great at predicting what people would do, and he ran lockdown decks that had to have come straight out of hell itself. You couldn't do anything. You couldn't win. "Chuuya and I worked quite a bit together on that Machine deck. It was tailored just for him, so I had a feeling." Dazai set the food down on bed. "Hey, love." He said, giving Chuuya a gentle smile. Chuuya pulled himself to a sitting position. "Oh, hey, aren't you supposed to be beating off in a public bathroom?" He teased. Dazai batted his hand. "Not if the alternative is celebrating with you."

He said it sweetly, as if that could save the fact that he'd basically admitted that he would be otherwise. Chuuya rolled his eyes, and Kajii and Dazai both joined him on the bed, kissing the top of his head together. They'd had this sort of relationship for a couple months now. Kajii didn't know Dazai terribly well, but he and Chuuya had a lot of history in the past. And Kajii had his own relationship with Chuuya. And Chuuya loved them both. Kajii had been a little jealous of Dazai at first, but he figured out quickly that Dazai wasn't competition. They weren't vying for affections, they merely shared them. And they got to make Chuuya smile together, and trap him in hugs, and hold both of his hands. He might not feel what Chuuya felt toward Dazai, but he'd decided that he didn't really need to. Dazai was his friend now, and perhaps in time he'd grow on him romantically. Even if he didn't; the relationship they had now was wonderful. It didn't need to change. "How badly did he beat you, Motojirou?" Dazai asked, pulling a slice of pizza from the box. "I lost with -750." He answered, taking a slice of his own. Chuuya smiled smugly. "I took all of his AAs and a Charger for Chimeratech." He added. "Nice!" Dazai said, but his congratulatory expression disappeared as Chuuya made to grab his pizza. "Get your own, pipsqueak." He said with a teasing laugh, holding it out of reach. "There's a whole box." Chuuya huffed. "I bet 'Jirou'd give me his." He pouted. "Of course, lemondrop."

Kajii took another slice from the box and handed his to Chuuya, smiling and earning himself a kiss on the cheek. He gave a small giddy laugh.   He had everything he could want. And with the way the night had gone, with Chuuya here, with Dazai here...with the game they'd played, and the expressions his boyfriend made, and the kisses he'd given and gotten —as far as he was concerned, that was all he needed. He was happy.

[jeramymobley]

Amazon Prime Day 2017 Tops Black Friday and Cyber Monday

Amazon is thrilled that its just-wrapped Prime Day sales event was its biggest global sales event to date. The shopping extravaganza surpassed its Black Friday and Cyber Monday tallies, combined.

According to Bloomberg News, “The 30-hour sale, featuring discounts on a broad assortment of goods, generated about $1 billion in revenue for the Seattle-based web retailer, according to separate estimates from analysts at Cowen & Co. and JPMorgan Chase & Co. That’s triple its average daily e-commerce revenue in 2016, according to data compiled by Bloomberg.”

And more importantly for Amazon, it boosted its Prime subscribers and sold more Echo devices to draw more customers into its ecosystem.

The Prime Day 2017 event grew by more than 60 percent over last year. Sales growth by small businesses and entrepreneurs was even higher, with some reporting their own booming Prime Day sales results.

More details in Amazon’s press release below:

On a day with incredible deals reserved exclusively for Prime members, sales on July 11 surpassed Black Friday and Cyber Monday, making it the biggest day ever in Amazon history. With hundreds of thousands of deals, this year’s Prime Day was too big for 24 hours – so Prime members had 30 hours to shop. The Prime Day 2017 event grew by more than 60 percent compared to the same 30 hours last year, and sales growth by small businesses and entrepreneurs was even higher. More new members joined Prime on July 11 than on any single day in Amazon history. Tens of millions of Prime members made a purchase on Prime Day 2017, more than 50 percent higher than the prior year.

Prime members’ most popular purchase was the Echo Dot, which was not only the best-selling Amazon device this Prime Day, but also the best-selling product from any manufacturer in any category across Amazon globally. Prime Day 2017 was also the biggest sales event ever for Amazon devices in the U.S. and around the world, including record sales for Echo, Fire tablets and Kindle devices. The all new Element 55-inch 4K Ultra HD smart LED TV – Amazon Fire TV Edition was the best-selling TV deal in Amazon history – selling through record units in less than four hours. Top sellers from around the world, excluding Amazon devices, included:

U.S.: Instant Pot DUO80 7-in-1 Multi-Use Programmable Pressure Cooker; 23andMe DNA Tests for Health + Ancestry U.K.: TP-Link Wi-Fi Smart Plug, works with Alexa; Sony Playstation 4 Spain: SanDisk Ultra Fit 64GB USB 3.0 Flash Drive; Moto G Plus (5th Generation) Smartphone; Lenovo Ideapad 310 Laptop Mexico: AmazonBasics Apple Certified Lightning to USB Cable; Nintendo Switch Japan: SAVAS Whey Protein; Happy Belly pure bottled water Italy: Finish All in One Max tablets; Caffe Vergnano 1882 Espresso machine India: OnePlus 5 phone; Seagate Expansion 1.5TB Portable External Drive Germany and Austria: PlayStation Plus Membership; Soda Stream France and Belgium: PlayStation Plus Membership; Game of Thrones – The Complete Season 1 to 6 Blu-Ray China: Fisher Price Soothe and Glow Seahorse; A brief history of humankind+ A brief history of tomorrow set Canada: Instant Pot Duo 7-in-1 Multi-Use Programmable Pressure Cooker, 8 Qt; AmazonBasics AA Rechargeable Batteries (8-Pack)

“To those customers who tried Prime for the first time and our long time members, thank you for a great Prime Day,” said Greg Greeley, Vice President, Amazon Prime. “Our teams around the world will keep working to add more and more to your membership, so Prime continues to make your life better every day. We are already looking forward to our Prime Day celebration next year.”

Global Highlights from Prime Day 2017

• A record number of Prime members shopped across 13 countries. • Prime members purchased seven times more Amazon Echo devices globally than on Prime Day 2016. • Members saved hundreds of millions of dollars on product discounts globally, compared to the already low prices offered to non-Prime customers. • More than 3.5 million toys were purchased by customers worldwide on Prime Day 2017. • Tens of millions of customers used the Amazon App on Prime Day. Customer orders on the Amazon App more than doubled this Prime Day compared to last year.

U.S. Highlights from Prime Day 2017

• Members found deals organized by more than 20 of the most-shopped-for themes – from Pet Lovers to Gardeners and more.       • The most popular themes on Prime Day were Home Chefs, Techies and For the Home. • Prime members purchased more than 50,000 TP-Link Smart Plugs. • The fastest Prime Now deliveries on Prime Day were in Sunnyvale and Berkeley, California and Kirkland, Washington to members who ordered snacks, writeable DVD packs and a Samsung Internal SSD. The orders were each delivered in 12 minutes. • More than 200,000 women’s dresses and more than 200,000 lightbulbs were purchased by customers on Prime Day 2017. • More than 100,000 items from Amazon Launchpad startups including the Anova Culinary Bluetooth Sous Vide Precision Cooker, Soylent Coffiest Meal Replacement Drink, Skybell Wi-Fi Video Doorbells and LIFX Wi-Fi Smart LED Light Bulb were orders by customers by on Prime Day 2017.

Prime Members Love Movies, Music and Books

In the week leading up to Prime Day 2017, Prime members enjoyed Prime Video, Prime Reading, Amazon Music and Audible, streaming and downloading more than two times compared to last year. Also in the week leading up to Prime Day, the most downloaded Kindle Unlimited book was Stillhouse Lake and the most streamed song on Amazon Music was “Something Just Like This” by the Chainsmokers & Coldplay.

Small Business and Entrepreneur Highlights from Prime Day 2017

Prime Day 2017 was another record-breaking success for small businesses and entrepreneurs worldwide who participated in the event. Feedback from small businesses and entrepreneurs includes:

“We’re absolutely thrilled – our daily sales increased by over 500 times on Prime Day. Within the first six hours, Furbo Dog Camera shot up to the No.1 Best Seller in four categories including the highly competitive pet supplies and home monitor category,” said, Victor Chang, Furbo Dog Camera, Bellevue, Washington.

“Prime Day was a huge success for us. We had a massive lift in sales last night compared to an average Monday night, and our deal sold out almost as soon as it went live. The level of demand far surpassed our expectations,” said Caron Proschan, Simply Gum, New York, New York.

“Prime Day has been another amazing success this year! Both Monday and Tuesday (Prime Day) were record days in sales for our business. It’s great to have the additional sales boost during these traditionally slower summer months,” said Tyler & Courtney LeCompte, Confetti Momma, Lafayette, Louisiana.

“Overall, for the Prime Day event (all 30 hours), we saw about 20 times more overall sales. This is a huge benefit to our business, as it gives us exposure to thousands of new customers, and helps us with cash flow as we ramp up on inventory for the Holiday Season,” said Ben Arneberg, Willow & Everett, Boston, Massachusetts.

“So far, this year’s Prime Day sales increased 350 percent from last year’s. Getting the opportunity to offer lightning deals as a Handmade at Amazon artisan has been a boom to my business,” said Marcia Ricchiuti, Kahili Creations, Pahoa, Hawaii.

“LightAccents had a fantastic Prime Day. It was our best sales day in over two years,” said Lawrence Bibi, LightAccents, based in New York, New York.

Prime Member Highlights from Prime Day 2017

We spoke to several Prime members who shopped on Prime Day and this is what they told us:

“When you’re a teacher, your first priority is, and always will be, your students. Thanks to Prime Day, I bought a lot of things, thinking of my students from this past year and this next year. Magnet letters to use for practicing spelling words, stools for them to sit on while working with me at a table, and many books. I also bought some Velcro “coins” to use in the class room. These are life savers!” said Laura McIntosh from Tucson, Arizona.

“After some browsing, I saw that the Fire HD 8 Tablet was on sale for $49.99. Having the newest version with Alexa capabilities, it will be perfect as I plan for my own wedding. I’m an event planner and also have three other weddings from the end of August until December. That is a lot of things to remember. The Fire Tablet can now be with me on commutes and at the airport and maybe I will start to read for fun again,” said Jade Barnes from Philadelphia, Pennsylvania.

“I could hardly believe the savings! Amazon proves time and time again that its reliability and product pricing simply cannot be beat. I am a very proud Prime member and cardholder and I cannot imagine my life without Amazon,” said Liana Babich from Warwick, Rhode Island.

  The post Amazon Prime Day 2017 Tops Black Friday and Cyber Monday appeared first on brandchannel:.

[immunofrontiers-blog]

Inovio Receives $5 Million Grant from Bill and Melinda Gates Foundation for COVID-19 Vaccine Program

In a recent press release, Pennsylvania-based American biotechnology company Inovio Pharmaceuticals (Nasdaq: INO) reported that the Bill and Melinda Gates Foundation has provided a $5 million grant to support the accelerated evaluation and production of their "CELLECTRA 3PSP" medical device, which is integral to the intradermal delivery of their DNA-based COVID-19 vaccine INO-4800. INO-4800 is currently being tested in preclinical studies and is anticipated to progress to Phase 1 clinical trials in the United States during April 2020. President and CEO of Inovio Dr. J. Joseph Kim stated, "INOVIO is grateful to the Bill & Melinda Gates Foundation for their continued investment in INOVIO's DNA medicines platform and for their support for DNA vaccines to potentially protect those at risk globally given the current COVID-19 outbreak. Our team of vaccine experts are working around the clock to advance INO-4800 and we look forward to attracting additional partnerships to expedite its development to meet this urgent global health need." According to the press release, "The next generation CELLECTRA 3PSP device is designed specifically for a COVID-19 type pandemic scenario. It is a small, portable, hand-held, user-friendly device that runs on readily available "AA" batteries. This allows for stockpiling of the device in quantity without maintenance. It is easy to use and is based on our current device with extensive history (over 6,000 administrations) which has received the CE mark and has an acceptable safety profile. The streamlined design also allows it to be readily produced at reduced costs and large scale." "The device has been designed with reliability, challenging environments, user needs and ease of large scale manufacturing in mind. INOVIO's San Diego Device Manufacturing facility will build initial quantities and demonstrate the design and scale up of manufacturing processes which can then be transferred to additional contract manufacturers for increased capacity. Initial development of CELLECTRA 3PSP was started in 2019 with $8.1 million funding from the medical arm of the U.S. Defense Threat Reduction Agency (DTRA)'s Medical CBRN Defense Consortium. The new funding will help to accelerate the testing and completion of the device development and scale up to combat the COVID-19 disease," the press release stated. Inovio intends to deliver one million doses of the INO-4800 vaccine by the end of 2020, and "is working to scale up both INO-4800 and CELLECTRA 3PSP devices to potentially make available millions of doses to combat this outbreak." The company has announced plans to start US-based human clinical trials of INO-4800 in April. During a meeting with the US Coronavirus Task Force on March 2nd at the White House, Inovio President and CEO Dr. J. Joseph Kim stated, "Inovio is the leader in coronavirus vaccine development and the only company with a Phase 2 vaccine for a related coronavirus that causes Middle East Respiratory Syndrome (MERS). Using our modern DNA medicines platform, we designed our DNA vaccine INO-4800 in three hours after the publication of the genetic sequence of the novel coronavirus that causes COVID-19." Stay aware of breaking immuno-biotech news and the latest advancements in the field of immunology. "We immediately began preclinical testing and small-scale manufacture and have already shared robust preclinical data with our public and private partners. We plan to begin human clinical trials in the U.S. in April and soon thereafter in China and South Korea, where the outbreak is impacting the most people. We plan on delivering one million doses by year end with existing resources and capacity. However, we will need additional resources to scale up to make enough doses to help protect Americans from COVID-19 as well as to lead global efforts to curtail this virus," continued Dr. Kim. On January 23rd, 2020, the Coalition for Epidemic Preparedness Innovations (CEPI) issued Inovio a grant of up to $9 million to fund preclinical and clinical development of INO-4800. CEPI CEO Richard Hatchett stated, "Given the rapid global spread of the 2019-nCoV virus the world needs to act quickly and in unity to tackle this disease. Our intention with this work is to leverage our work with Inovio on the MERS coronavirus and rapid response platform to speed up vaccine development." Dr. Kim responded, "We're extremely honored to expand our partnership with CEPI to tackle this new threat to global public health. Our DNA medicine platform represents the best modern day approach to combatting emerging pandemics. We have already demonstrated positive clinical outcomes with our vaccine against MERS-CoV, another coronavirus. Importantly, following the Zika viral infection outbreak, Inovio and our partners developed a vaccine that went from bench to human testing in just seven months – the fastest vaccine development on record in recent decades. We believe we can further improve upon this accelerated timeline to meet the current challenge of the emerging Chinese coronavirus 2019-nCoV." On January 30th, 2020, Inovio announced that it would develop INO-4800 in collaboration with China-based Beijing Advaccine Biotechnology Co., with the goal of expediting the testing of INO-4800. Dr. Kim stated, "Our collaboration with Beijing Advaccine and its Founder, Emeritus Professor Bin Wang from the prestigious Fudan University and China's premier DNA vaccine expert, will tremendously accelerate our coronavirus vaccine INO-4800 development in China because of its expertise and experience with regulatory authorities and clinical trial management. This collaboration allows us to enter China and deliver our vaccine into the areas where they need it most as soon as possible. Our shared goal is to utilize both company's expertise in developing vaccines for emerging infectious diseases and hopefully achieve an accelerated regulatory approval for INO-4800." Since January, Inovio Pharmaceuticals has demonstrated immune responses to their vaccine in animal models and initiated plans for a human clinical trial. The company intends to finalize their clinical trial design in March and prepare 3,000 doses of their vaccine for trials in the United States, South Korea, and China. The first clinical trial will include 30 healthy volunteers in the United States, with the Asia-based trials occurring afterwards. Inovio's DNA-based vaccine platform is centered around speed of manufacturing, stability during transport and storage, and efficacy for inducing a robust immune response. INO-4800 consists of small circles of engineered DNA that can be directly injected into cells through the controlled use of electricity, which creates temporary pores in the membranes of target cells and allows the double-stranded DNA circles contained within the vaccine to enter cells, alter their activity, and eventually lead to an immune response. Since Inovio's formulation is composed only of highly purified DNA, water, and salt, it avoids potential toxicity and can be manufactured relatively quickly and stored and transported without freezing. During the epidemic of the Middle East Respiratory Syndrome (MERS), a coronavirus related to the virus that causes COVID-19, Inovio was the first biotechnology company to test a vaccine against the MERS virus (INO-4700) in humans. The company plans to further evaluate INO-4700 in a Phase 2 clinical trial in the Middle East, where the majority of MERS cases have occurred. Stay aware of breaking immuno-biotech news and the latest advancements in the field of immunology. The results of a Phase 1 clinical trial evaluating the immunogenicity, safety, and tolerability of the MERS coronavirus vaccine GLS-5300 (INO-4700), published in the journal Lancet Infectious Diseases, demonstrated that their DNA-based vaccine technology is well-tolerated and capable of inducing an antiviral immune response. After two vaccinations, 47 of 66 (71%) study participants showed evidence of a T-cell response. 44 of 58 (76%) participants demonstrated a T-cell response after three vaccinations. Seroconversion was detected by S1-ELISA in 59 of 69 (86%) and 61 of 65 (94%) participants after two and three vaccinations, respectively, and 34 of 68 (50%) individuals were found to bear neutralizing antibodies. No serious adverse events were associated with the use of the MERS coronavirus vaccine. The authors stated that the antibody and cellular immune responses associated with the vaccine were durable and similar to those of patients that recovered naturally from infection by the MERS coronavirus. The Bill and Melinda Gates Foundation, founded in 2000, has financially supported a variety of developments related to human health, social sciences, and education. Disclosure: We do not have positions or business relationships with the stocks or companies mentioned in this article. This article does not in any way constitute a recommendation. Please refer to the statement on Forward Looking Statements in the press release. Modjarrad K, Roberts CC, Mills KT, et al. (2019) Safety and immunogenicity of an anti-Middle East respiratory syndrome coronavirus DNA vaccine: a phase 1, open-label, single-arm, dose-escalation trial. Lancet Infectious Diseases, 19(9), P1013-1022. DOI: https://doi.org/10.1016/S1473-3099(19)30266-X Read the full article

[electricoutdoors]

LifeStraw vs SteriPEN – Their Strengths and Weaknesses

LifeStraw vs SteriPEN

Water filters are essential for backpackers, campers and survival kits. Two of the most common are the LifeStraw and SteriPEN. At first glance, they may seem like they’re very similar, but they’re actually very different.

What’s the difference between the LifeStraw and SteriPEN? The LifeStraw is an easy to use straw style water filter that removes bacteria and protozoa. The SteriPEN is a UV water purifier which kills bacteria, protozoa, and viruses 1 liter of water at a time, but doesn’t filter water.

Let’s look at the strengths and weaknesses of the LifeStraw and SteriPEN so you can figure out which one is going to be best for you. [wc_toggle title=“Table of Contents” padding=“” border_width=“” class=“” layout=“box”]

LifeStraw vs SteriPEN

LifeStraw or SteriPEN - Which Should You Choose

LifeStraw

Do LifeStraws really work?

Can you drink any water with the LifeStraw?

How many times can you use a LifeStraw?

Do LifeStraws remove viruses?

How do you clean a LifeStraw filter?

What kind of filter does LifeStraw use?

SteriPEN

Does the SteriPEN really work?

How does UV light kill bacteria?

Can you drink any water with the SteriPEN?

How long does a SteriPEN last?

What kind of batteries does a SteriPEN use?

Can you use a SteriPEN in a metal bottle?

LifeStraw vs SteriPEN - Which is Best for You

Do you need to treat your water for viruses?

Most people will be better off with the LifeStraw

Conclusion

[/wc_toggle]

LifeStraw or SteriPEN - Which Should You Choose

When you first look at the LifeStraw and SteriPEN you may think that they’re similar to one another, but they’re actually completely different! The LifeStraw is a water filter and the SteriPEN is a water purifier.

.tg {border-collapse:collapse;border-spacing:0;} .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:black;} .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:black;} .tg .tg-c3ow{border-color:inherit;text-align:center;vertical-align:top} .tg .tg-0pky{border-color:inherit;text-align:left;vertical-align:top}

LifeStraw vs. SteriPEN LifeStraw SteriPEN Dimensions (W x H x D) 9" x 1.2" Dia 7.3" x 1.6" x 1.3" Weight 1.62 oz. 5 oz. Does it remove Protozoa/Bacteria Yes Yes Does it remove Viruses No Yes Filter Type Hollow Filter Membrane UV-C Sterilization Battery Type N/A Internal Battery Life N/A 50 Liters of Water Interface N/A USB Filter/Lamp Life 1000 Gallons 1000 Gallons

LifeStraw

The LifeStraw is the original straw-style water filter designed for hikers, bikers, and other people that enjoy the outdoors. It removes 99.999% of bacteria, parasites, and microplastics.

Length: 9″

Diameter: 1.2″

Weight: 1.62 oz.

Filter Life: 1,000 Gallons

Do LifeStraws really work?

LifeStraws work extremely well. They remove more than 99% of all bacteria and parasites. This means it takes out 99.999999% of waterborne bacteria (this includes e.coli and salmonella) and 99.999% of waterborne parasites (including giardia and cryptosporidium).

It also removes all but the smallest microplastics and sediment.

Can you drink any water with the LifeStraw?

LifeStraws are a filter so they remove the sediment, bacteria, and protozoa from the water before you drink it. This means that you can grab a gross, dirty glass of water and just drink it through the LifeStraw without a problem. LifeStraws don’t filter fuel and other chemicals out of the water so you need to be sure that there isn’t any chemical contamination in the water that you plan to drink.

They also don’t remove viruses. If you suspect that the water has viruses of some kind in it, then you need to go with some other method of getting it clean enough to drink. If this is the case you can boil the water before drinking it through the LifeStraw. This will kill the viruses (as well as the protozoa and bacteria).

It also will not filter out salt from water. So while it may remove the bacteria and any parasites from saltwater, you still can’t safely drink it unless you remove the salt some other way.

How many times can you use a LifeStraw?

The long life of the LifeStraw is one of its best selling points! You can filter about 1,000 gallons of water with one LifeStraw. That’s about 5 years worth of water.

[wc_box color=“primary” text_align=“left” margin_top=“” margin_bottom=“” class=“”]

You can read our entire LifeStraw review here.

[/wc_box]

Do LifeStraws remove viruses?

LifeStraws can’t remove viruses. Viruses are too small to be filtered out by most filters including the LifeStraw.

How do you clean a LifeStraw filter?

LifeStraws can’t be broken down and cleaned like some other water filters. The best that you can do is to blow out the LifeStraw after each use and leave both ends open so the filter can dry out.

You can also suck some clean water up into the filter and then blow it back out. Of course, you need to have clean water sitting around for this to work.

When you’re using a LifeStraw, you need yo keep in mind that the end that goes into the water can possibly be contaminated. Keep it out of any clean water you have unless you don’t care that you can contaminate it.

What kind of filter does LifeStraw use?

LifeStraws use a hollow filter microfiltration membrane style filter. Water is forced through the .2 micron pores of the filter as you suck up water.

SteriPEN

The SteriPEN is a lightweight UV water purifier designed to quickly make water clean and safe for drinking. It eliminated bacteria and protozoa like most water filters, but it also kills viruses which filters can’t.

Weight: 5 oz.

Length: 7.3″

Width: 1.6″

Bulb Life: 1,000 Gallons

Does the SteriPEN really work?

The SteriPEN kills 99.9% of all bacteria, protozoa, and viruses. It either flat out kills or deactivates them which means that they can’t reproduce or make you sick.

This big difference is that the SteriPEN doesn’t filter anything out. You’ll deactivate or kill anything in the water, but you’re still going to drink it along with any sediment that’s in the water.

One of the best things that UV sterilization does (or doesn’t do) is not add any chemicals to your water. If this important to you, then you’re really going to like the SteriPEN!

How does UV light kill bacteria?

UV light is a type of radiation. UV-C, the type used to disinfect water, is normally blocked by the ozone but bulbs like the one in the SteriPEN produce UV-C because it’s so effective in stopping microorganisms.

When these microorganisms are hit by the UV light, it damages their DNA and stops them from being able to reproduce. This means that you can’t get sick from them because they need to be able to reproduce to make you sick. By reproducing in your body they eventually cause negative health effects.

Can you drink any water with the SteriPEN?

The SteriPEN kills or damages microorganisms. That’s it. This means that you can’t drink chemically contaminated water or water that has salt in it.

How long does a SteriPEN last?

The SteriPEN can disinfect 50 liters of water per charge and the bulb life lasts for about 8000 activations. 1 activation disinfects .5 liter and 2 activations disinfect 1 liter of water.

What kind of batteries does a SteriPEN use?

This SeriPEN has a rechargeable battery that comes as part of the unit. The USB charging port allows you to recharge the SteriPEN with any of your USB power packs or solar panels. This makes it great for me since I usually have a solar panel and battery pack with me.

You can find other versions of the SteriPEN that use AA batteries if you like batteries that can be replaced better. There are benefits of both so go with what works for you!

Can you use a SteriPEN in a metal bottle?

SteriPENs can be used in pretty much any container that you have. Glass, metal, plastic, etc. The container actually blocks any possible harmful UV light that gets produced.

LifeStraw vs SteriPEN - Which is Best for You

Choosing between the LifeStraw and SteriPEN can be confusing but it’s actually pretty straight forward in my opinion.

Do you need to treat your water for viruses?

Most of the worst waterborne diseases that you’re going to find in North America are caused by either bacteria or protozoa. This means that you’re probably not going to need to kill off viruses in your drinking water. The one exception is the possibility of Hepatitis A.

Hepatitis A is spread when human feces from an infected person make it into a water supply. Hepatitis A is found all across the U.S. but it’s rare for it to be found in water supplies.

If you think you’re likely to contact water that’s contaminated by human waste, then the SteriPEN is what you want.

Most people will be better off with the LifeStraw

Anyone looking for an inexpensive filter that is easy to use and works well in cloudy water should go for the LifeStraw.

Conclusion

The LifeStraw and SteriPEN are two common pieces of equipment used to make potentially contaminated water sources clean and drinkable.

Both are small and pretty easy to use, but the similarities really end there. The LifeStraw is a personal water filter and the SteriPEN is a UV water sterilizer that can make drinking water for an individual or a group.

In the end, I’d suggest going for the cheaper LifeStraw over the SteriPEN unless you need to treat water for multiple people or you think you’re going to need to treat water for viruses.

The blog post LifeStraw vs SteriPEN – Their Strengths and Weaknesses is republished from: https://readylifestyle.com/

LifeStraw vs SteriPEN – Their Strengths and Weaknesses published first on https://readylifesytle.tumblr.com

[swldx]

6070Khz 1157 16 MAR 2021 - CFRX TORONTO, CFRB 1010 (CANADA) in ENGLISH from TORONTO (AURORA) ON. SINPO = 55122. English, ID @1200 fb "8:00 news" anchored by Ashleigh Legassic. Freezing and snow currently but should thaw over the rest of the week. @1202z "timesaver traffic" report and sponsor mentioned. Ontario [Covid19] Vaccine Rollout: male interviewed "no matter what vaccine is available, get it!" Pre-trial hearing about a 10 year old abduction and murder case with new DNA evidence. @1206z musical interlude fb male announcers conversing about growing diversity in country music following the recent "Black Lives Matter" movement. Also discussion about only frontline people like restaurant waiters, etc. really need the Covid19 vaccine. @1215z "timesaver traffic" update. MLA 30 amplified loop (powered w/8 AA rechargeable batteries ~10.8vdc), Etón e1XM. 1kW, Omni, bearing 94°. Received at Plymouth, United States, 1116KM from transmitter at Mississauga (Clarkson, 43°30′39.67″N 79°37′48.07″W) ON. Local time: 0657.

[batterycondition]

Mt Washington Tent. A GREAT TENT for Moto Camping. by CameraGirlUSA

Wanderlust… That’s what they call it. The driving need to get out and see new places and enjoy the journey. I have had Wanderlust all my life. I think some people are just born with it in their DNA. I am certain I stood twice in that line. So, I have been making my lists for packing up the Vespa and heading out of town this next weekend. Lists for me and for the Vespa…extra battery, extra sparkplug, spare fuses, tire pump kit, scooter repair tools , etc. All this is a solo-dry-run for the beachside camping I have planned for later this Spring. I am so lucky to have a husband that truly understands me and is OK with my need for solo adventures and personal growth time. Life Is Good…Living Your Best Life Is Even Better!

PHOTO CREDIT: This photo was taken by Gigatent as a product promo photo. I am simply showcasing it here because I own this tent. I hope they don’t mind as I am signing the praises of their product. I will be using my tent for moto-camping adventures this 2014 Spring – Fall. #reconditioning #batteries car battery, #battery, rechargeable aa batteries, rechargeable batteries, battery charger, car batteries, nimh battery, battery doctor, battery reconditioning, battery repair, battery desulfator @BatteryReconditioningNew Home http://bit.ly/2JXxDmj

The post Mt Washington Tent. A GREAT TENT for Moto Camping. by CameraGirlUSA appeared first on Battery Reconditioning.

from Battery Reconditioning http://batteryreconditioning.onlinestorewebpro.com/mt-washington-tent-a-great-tent-for-moto-camping-by-cameragirlusa/?utm_source=rss&utm_medium=rss&utm_campaign=mt-washington-tent-a-great-tent-for-moto-camping-by-cameragirlusa

[kathleenseiber]

Super cheap centrifuge device started with a toy

A 5,000-year-old toy has inspired an inexpensive, hand-powered centrifuge for preparing scientific samples including DNA.

The scientific tool could not only affect how field biologists conduct their research but also allow high-school students and others with limited resources to realize their own state-of-the-art experiments.

The researchers demonstrated the device—dubbed the 3D-Fuge because it is the product of 3D printing—in two separate ways.

In a rainforest in Peru the 3D-Fuge was an integral part of a “lab in a backpack” that researchers used to identify four previously-unknown plants and insects by sequencing their DNA.

Back in the United States, a slightly different design enabled a new approach to creating living bacterial sensors for the potential detection of disease. That work took place at Lambert High School in Georgia for a synthetic biology competition.

Spreading the idea

Thanks to social media and a preprint of the PLOS Biology paper on the device on BioRxiv, the 3D-Fuge has already generated interest from around the world, including emails from high-school teachers in Zambia and Kenya.

“It’s awesome to see research not just remain isolated to one location but see it spread,” says co-first author of the paper Gaurav Byagathvalli, a senior at Lambert High School in Georgia, who worked with M. Saad Bhamla, an assistant professor at the Georgia Institute of Technology.

“Through this, we’ve realized how much of an impact simple yet effective tools can have, and hope this technology motivates others to continue along the same path and innovate new solutions to global issues.”

To better share the work, the team has made the 3D-Fuge designs, videos, and photos available online.

Frugal science

One focus of Bhamla’s lab is the development of tools for frugal science, or real research that just about anyone can afford. The tools behind state-of-the-art science often cost thousands of dollars that make them inaccessible to those without serious resources.

Centrifuges are a good example. A small benchtop unit costs between $3,000 and $5,000; larger units cost many times that. Yet the devices are necessary to produce concentrated amounts of, say, genomic materials like DNA. By rapidly spinning samples, they separate materials of interest from biological debris.

The Bhamla team found that the 3D-Fuge works as well as its more expensive cousins, but costs less than $1.

3D-Fuge origins

The 3D-Fuge is based on earlier work by Bhamla and colleagues at Stanford University on a simple centrifuge made of paper. The “paperfuge,” in turn, takes inspiration from a string and button toy that Bhamla played with as a child. He later discovered that these toys, known as whirligigs, have existed for some 5,000 years.

They consist of a disk—like a button—with two holes, threaded with a length of flexible cord whose ends are knotted to create a single loop with the disk in the middle. The user swings that simple contraption with two hands until the button is spinning and whirring very quickly.

The earlier paperfuge uses a disk of paper. To that disk Bhamla glued small plastic tubes filled with a sample. He and colleagues reported that the device did indeed create high-quality samples.

In late 2017 Aaron F. Pomerantz, a graduate student at the University of California, Berkeley, approached Bhamla, as did the Lambert High team, to see if the paperfuge could adapt for the larger samples they needed (the paperfuge is limited to small samples of ~1 microliter—or one drop of blood).

Together they came up with the 3D-Fuge, which includes cavities for tubes that can hold some 100 times more of a sample than the paperfuge. The team developed two equally effective designs: one for field biology (led by Pomerantz) and the other for the high-school’s synthetic biology project (led by Byagathvalli).

Bhamla notes that the 3D-Fuge has some limitations. For example, it can only process a few samples at a time (some applications require thousands of samples). Further, because it’s 10 times heavier than the paperfuge, it can’t reach the same speeds or produce the same forces of that device. That said, it still weighs only 20 grams, slightly less than an AA battery.

“But it works,” says Bhamla. “All you need is an [appropriate] application and some creativity.”

The National Science Foundation, the Mindlin Foundation, and the Jacobs Institute Innovation Catalyst Award funded the work.

Source: Elizabeth Thomson for Georgia Tech

The post Super cheap centrifuge device started with a toy appeared first on Futurity.

Super cheap centrifuge device started with a toy published first on https://triviaqaweb.weebly.com/

[lazuliquetzal]

🌈 What inspired you to write AA Batteries?

AH OKAY... this isn't really a secret but I write self-inserts. Like, the over-the-top, BAMF, indulgent, #cringe self-inserts. It's fun, it makes writing fun, and sometimes, every once in a while, I reread them and I think, "hey, there's a story here! I bet someone other than me would enjoy it!"

... so, uh, yeah. Akira's an updated and fleshed-out version of a self-insert fic I wrote when I was 14. He's basically 14-year-old me, except, like, he's a dude who plays baseball. Do not psychoanalyze this boy, because he is me.

The other thing that inspired me is that OCs get a horrible rep in fanfiction, which is a shame because they are GREAT and I LOVE THEM ALL TO PIECES. My one goal with AAB is to convince people that OCs are good, actually. I hope it worked lmao.

[farmgardenstore-blog]

Dancing DNA Kinetic Mobile - With crystal balls on the ends

Dancing DNA Kinetic Mobile - With crystal balls on the ends

Dancing DNA – Kinetic Mobile – With multi-colored Polymer balls on the ends – Enhances and enlivens any space: Home, office, kid’s room, waiting area, treatment room, breakfast nook, solarium, dorm room or class room. Enjoyed and valued by all – young and old! Includes mobile, motor and instructions. Uses 3 AA Batteries . Measures about 54″ when hanging. WARNING: Possible choking hazard. Keep out of reach of small children. Care and feeding: Gently dust to keep it clean. Change top to bottom, bottom to top every six months or so. If taking it down and moving it, grasp not just by hook, but by hook and again 6″ on neoprene spine below hook. To store, roll up with tissue paper as a sushi roll, make sure that the spine is not twisted. Color- Multi Color. INDOOR USE ONLY Includes Twirling Machine Motor View all from DSA

Read more on https://farmgardensuperstore.com/product/dancing-dna-kinetic-mobile-with-crystal-balls-on-the-ends/

[infotainmentplus-blog]

Worst smartphone experiences: Our team members share their tales We’ve been through many smartphones here at Android Authority — it comes with the territory. Some have been great, some have been bad. But how bad can it get? We asked our AA staff to recall their worst smartphone experiences of all time, highlighting the best ones below. We tried to stay clear of low-end smartphones because, let’s face it, most ultra-cheap Android devices were awful for the longest time. HTC leads the pack One of the more interesting tidbits from the poll was how HTC surfaced three times. This could be due the sheer bad luck of our staff, HTC’s fault, or its early popularity magnifying bad smartphone experiences. It’s still rather interesting. More specifically, Oliver Cragg‘s worst experience was the HTC One M9. “Imagine following the HTC One M8 (one of the most beautiful and best smartphones ever made) with a buggy, derivative phone that had an unforgivably bad camera,” Oliver explains. “I had it for six months before it stopped accepting any kind of network — a blessing in disguise. I’ve used HTC phones since (the 10 and U11 were both solid), but the M9 was a disaster.” Editor's Pick Daily drivers: 30 AA team members reveal the phones they use Mobile tech is what we do here at Android Authority. We have a huge team of people from all over the world. With that comes a broad range of needs and wants when it comes … Sticking with HTC, our SoundGuys colleague Lily Katz particularly disliked with 2012’s Droid DNA. “There was constant screen flickering and sporadic reboots that weren’t remedied by multiple factory resets. Not cute,” Lily said. Going back even further, Jimmy Westenberg pointed to 2010’s HTC Desire, but his experience wasn’t as bad as you might expect. His only major complaint was the storage space — a common issue during this era. “I could pretty much only use the stock HTC apps — the 4GB of storage didn’t allow for much wiggle room. I got used to living my life with a ‘low storage’ notification, which was annoying,” Jimmy recalled. “Other than that, the phone was great! No iPhone killer, but I still have good memories with it.” Huawei makes two appearances Chinese brand Huawei surfaced twice. The manufacturer is massive in Europe, China, and emerging markets, so it shouldn’t be surprising someone had a bad experience. Scott Adam Gordon generally had a good experience with his phones, but says the Huawei P10 sits atop the pile for worst smartphone experiences. It’s a stark contrast to the Huawei P20, which has been praised by us and other outlets. “They’ve all been great, but my worst experience has been with my current daily driver, the Huawei P10. The rear cameras have an autofocus problem that will cost money to fix (I don’t have a warranty),” said Scott. “The shots are blurry unless I take them in black and white. Google Photos will forever remember this as the film noir period of my life.” Read more: Best smartphones of 2018 — Here are our current picks Meanwhile, AA and DroneRush‘s Jonathan Feist mentions the Huawei Nexus 6P as his worst smartphone experience to date. It’s no surprise —the phone’s various issues have been well publicized. “It was a perfectly good phone for the first while, but then the issues started. First the battery would die anywhere below 50 percent, giving me less than two hours of screen-on-time per charge,” Jonathan says. “Then the dreaded boot loop killed it. To date, this is the only phone I have ever had that is no longer operational.” Did your old phone make the list? Robert Triggs recalled his experience with the Xperia X10, back when Sony Mobile was Sony Ericsson. “The Timescape UI was an overly complex, stuttering mess that got in the way more than it helped. Worse still, the Snapdragon S1 CPU developed an overheating problem after a year or so that kept rebooting the phone. I haven’t let a rubbish Android Donut experience put me off Sony, although I haven’t owned an Xperia since.” Meanwhile, the Oppo R11 (pictured above) was the source of Tristan Rayner‘s frustration. “The hardware wasn’t awful, but it came without an oleophobic coating, copied iPhone-style anti-Android notifications and had a poor skin,” said Tristan. “Add to that very little third-party support from the likes of LineageOS because it didn’t sell well enough, plus Oppo moved across to the R11s, meaning it’s a phone no one wants and it can’t be re-sold easily either.” Samsung only showed up once in our poll, with C. Scott Brown‘s worst experience being his Galaxy S6 (above). The device saw Samsung attempt to take on Apple for design prowess, but that’s not why it landed on our list. “I hate TouchWiz/Samsung Experience with a fiery passion. I bought the S6 planning to flash CyanogenMod, but Sammy/AT&T locked the bootloader, leaving me with only module options. I returned it after only a few weeks and got the Nexus 6P instead. CyanogenMod was up and running in a few minutes.” Xiaomi‘s Mi Mix 2 gave Adam Doud his worst experience, partly because of the expectations tied to a high-end device. “This is a flagship-level phone but the camera was below average in almost every way — very disappointing. It also had a weird bug where it wouldn’t accept group SMS messages, which, for a suburban dad, is an automatic deal breaker.” If I had to pick my own worst experience, I’d choose 2009’s Nokia N900 (though it’s probably a toss up between it and the Lumia 950). On the plus side, it had a slideout QWERTY keyboard, 32GB of storage, powerful Maemo OS, FM transmitter, video-out, and could run the Debian distribution of Linux. But it lacked WhatsApp, used a Linux-style app repository system, offered a resistive touchscreen, and wasn’t meant to be used in portrait orientation. Did the experience deter them from the brand? One rather interesting figure was that just over 30 percent of our staff said their terrible smartphone experience soured them on the brand. It’s a tiny sample size of course — we only had roughly a dozen responses in total. Nevertheless, this is potentially good news for companies that produced recent stinkers — maybe people are as willing to overlook a misstep as most of our staff. Most of these brands are more recognizable though, so who knows whether users would be kind to unknown manufacturers. What was your worst smartphone experience? Did you jump ship to a different brand afterwards? Let us know in the comments section! , via Android Authority http://bit.ly/2yNw84D