#hplg

[cobrebuffalo]

Sakura Card Captors

Parte 1 

.avi - español

01 - Sakura y el Misterioso libro mágico http://lyksoomu.com/hPec 02 - La Preciosa amiga de Sakura http://lyksoomu.com/hPfg 03 - La Primera Cita De Sakura http://lyksoomu.com/hPga 04 - Un Domingo Muy Agitado http://lyksoomu.com/hPhA 05 - Sakura Un Panda Y Una Hermosa Tienda http://lyksoomu.com/hPiJ 06 - Los recuerdos de sakura y su mama http://lyksoomu.com/hPiy 07 - El primer desafio de Sakura como ladrona http://lyksoomu.com/hPjz 08 - El rival de Sakura http://lyksoomu.com/hPki 09 - Sakura y el broche misterioso http://lyksoomu.com/hPlG 10 - sakura y un evento deportivo lleno de flores http://lyksoomu.com/hPlz

[konekochugirl-blog]

I figured I’d post about this in case anyone is wondering from having completed chapter 4 of Harry Potter and the Lost Girl and are wondering who Adrian Pucey is. He is a minor character only mentioned a couple times in the HP series during Quidditch matches against Slytherin as he plays chaser.

[lafocuse]

🥂Practical And Modern Addition 💫HD Glass And Big Arabic Numerals 🪐Vintage Silent Metal Analog Alarm Clock ✨Know more✨ http://bitly.ws/hpLG

[macd1000]

#trees #season #nature #color #hiking #landscape #park #photo #camera https://www.instagram.com/p/CNQbAa-hPLG/?igshid=1eqqjzaqb7anb

[actidermmel]

ActiLabs have a SALE!

ActiLabs have a SALE!

We have an awesome Flash Sale! I just wanted to tell you about a Flash Sale that the bosses have announced that started yesterday! We have lots of gorgeous products on special offer, just while stocks last. These bargains are not expected to be around for long, so move quickly!! Check out the sale products below   Micellar Lotion   A mild aromatic cleanser for face, eyes and lips, is particularly…

[eugeniomoretti]

#acqua #diana #grottammare #clickfor_grottammare #clickfor_marche #byeù (presso Grottammare) https://www.instagram.com/p/CPYqJS-hplG/?utm_medium=tumblr

[uromotoutoka]

🌸 桜流し #film #walz35_sv https://www.instagram.com/p/Bxj2sv-Hplg/?igshid=1o0qyizolbcth

[shilo-zoo]

She has always been super shy. #leopardgecko #gecko #reptile #reptiles #reptilesofinstagram #geckos #geckosofinstagram #lizard #animals #lizards #pet #leopardgeckosofinstagram #leopardgeckos #lizardsofinstagram #petsofinstagram #pets #animal #beardeddragon #reptilelover #cute #geckolove #leo #exoticpets (at California) https://www.instagram.com/p/BwTymU-HpLG/?utm_source=ig_tumblr_share&igshid=18pt5pj6h0k7g

[regenerationman-blog]

Organs Need Blood!

The rapid development among research in regenerative medicine is revolutionizing the way to view organ transplants, and is improving the ability to repair and replace human tissue. One of the latest improvements is allowing us to solve the issue regarding organ failure, which is a leading healthcare challenge in the western world. There are approximately 6,000 people waiting for an organ transplant in Canada today, and there are an increasingly low number of donors to keep up with the high demand, therefore we must resort to other sources such as lab-grown organs. Although lab-grown organs are an efficient alternative, they are challenging to create, and even more challenging to maintain in the body. However, recently researchers at the University of Bath have discovered a potential method of keeping lab-grown organs alive and accepted into the body. This involves the use of the patient’s own blood in which creates a vascular network around the new lab-grown organ.

Creating lab-grown organs and tissues for transplant into patients is a priority in medicine and researchers in regenerative medicine are getting close to unlocking this potential. However, all this research is useless if we cannot get the body to accept the transplant and allow it to grow. These problems are a major challenge, and to solve them we need to figure out a way to provide new organs with a network of blood vessels that connects to the patients vascular system. This will allow the organs to survive and integrate into the recipient seamlessly.

Other methods previously used to overcome this problem involve the use of animal-derived hormones to stimulate vascularization. Using animal-derived hormones only have the capacity to form minimal capillary like structures and not actual vascular networks, which makes the organs less likely to survive when transplanted.  Additionally, since this method uses animal derived products this makes it harder for approval of clinical trials due to the risk of animal disease transmission and increases the likelihood of rejection by the patient.

A study done by researchers at the University of Bath, in the United Kingdom, have discovered a different way to stimulate blood vessel formation and their approach relies on the use of two materials. The first is human platelet lysate gel (hPLG), which is the extracellular matrix from human platelet cells used as a 3D scaffold to support the proliferation and vascularization of tissues. The other material is endothelial progenitor cells (EPCs), which are a type of stem cell that helps maintain blood vessel walls by regenerating into the endothelial lining of blood vessels. It is important that both of these materials are isolated from the patient to avoid rejection by the immune system. Meaning that when the organ is introduced into the body it is not recognized as foreign and makes it easier for the body to accept it.

EPCs grow and differentiate into the endothelial cells lining blood vessels, and when these researchers placed them in a 3D capsule of hPLG this caused the formation of a capillary vascular network. To create hPLG they extract blood from a patient, purify platelets, and sonicate to lyse them. Next they add a protease thrombin to cleave the lysate and form the gel (figure 1). Additionally they wanted to see if this method could create a vascular network around organs. To do this they used a cross section of a mouse aorta and placed it in the hPLG 3D capsule and allowed it to incubate for 3 days at body temperature (figure 1). Interestingly, this caused high amounts of capillary’s sprouting from the aortic ring.

Figure 1 – Process of creating human platelet lysate gel (hPLG) used for blood vessel formation of mouse aorta section with endothelial progenitor cells (EPC). A) Extraction of blood and purify platelet cells. B) Sonication of platelet cells to lyse them. C) Addition of thrombin to make hPLG then add EPCs and mouse aorta section. D) Blood vessel formation around mouse aorta ring section after 3 days.

The ability of hPLG to support the formation of vascular capillaries by EPCs has never been studied, prior to this, and is ideal for creating vascular networks for organs. New work is being done to test hPLG/EPCs success in other tissues and eventually lead to clinical trials.

The basis for their idea came from an earlier study that use platelet lysate liquid from patients, and showed to be a natural reservoir for growth factors to promote tissue repair, including blood vessel formation. These researches used the platelet lysate liquid as the media for EPCs to grow. However, the results from that did not show any significant blood vessel formation or proliferation of EPCs. From these results the researchers at Bath reasoned it would be useful to create a natural scaffold in order for EPCs to develop into blood vessels. They did this by turning the platelet lysate liquid into a 3D gel using the natural protease thrombin.

Next the researchers at Bath looked into how hPLG causes increase proliferation and blood vessel formation in EPCs. They discovered there are many growth factors released from platelets, one with importance is known to stimulate blood vessel formation in vivo and is known as vascular endothelial growth factor (VEGF). This growth factor interacts with the VEGF receptor (VEGFR2) on the EPCs and using an inhibitor against VEGFR2 to block its affect, it was pinpointed as the underlying signal that causes the response. However, they could not uncover the whole pathway on how it causes increases blood vessel formation and proliferation. They hypothesize that the 3D scaffold created by the hPLG extracellular matrix orients the receptors on EPCs in a way that increases their sensitivity to VEGF and causing blood vessel formation through activation of transcription factors (figure 2).

Figure 2 – Predicted signaling transduction pathway of vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) causing cell proliferation and blood vessel formation.

Using hPLG they are able to deliver growth factors where needed and induce more efficient formation of capillary vascular networks. This will increase the nourishment to the organ and increase its likeliness of survival.

The beauty of this new approach is that components of a person's own blood could be manipulated to create a scaffold for new blood vessels. This increases the likelihood that the new tissue will be integrated into the patient's body. However, additional research is needed to support this, and if proven successful, could improve the lives of many people by being able to engineering organs equipped with a functional vascular network. Maybe one day we will no longer need to rely on the organs we are born with and will look back on this study in history as the stepping-stone responsible for creating viable lab-grown organs.

[spilledinkbyrjay]

#howharddidpubertyhityou Kept the baby face but the drip went through stages . . . Hashtagged(R.D.Johnson 3) #poem #poetry #read #thoughts #wearabletherapy #reading #reader #page #pages #ybp #youngblackpoets #rdjohnson #library #author #writing #bookworm #mymind #love #prose #imagine #plot #spilledinkbyrjay #story #literature #literate #stories #words #quote #radpresspublishing *** https://www.instagram.com/p/BqEHqS-hpLG/?utm_source=ig_tumblr_share&igshid=1b8cxo4c0kvul

[acti-lab-ayesha-blog]

Check out our offer on our lip trio for unlimited offer. Includes the Le Joli Crayon, Mega Matte and the HPLG-Honey Twisted for only £22.40 plus shipping £4.50! Need any information on the products or site? Message me. Get at this deal ASAP before it disappears! Definitely worth it. #lip #products #recommended #offer #trio #worthit #crayon #mattelipstick #matte #lipgloss #limitedoffer

[hplg247]

HPlg: Pottermore

Soooooo I GOT INTO POTTERMORE!!!!!!!!!!!!!!!!!!!!!! On day 3 so not bad. I'm just getting to writing about it now cause i have been traveling so yeah stayed up late then got on a plane. Needless to say i slept long and hard the next night. Have not got the welcome letter yet but here is my username which i think is a good one.

MarauderQueen62

[konekochugirl-blog]

This is the courtyard I pictured and where Snape’s and Alexa’s first encounter took place in chapter two.

[konekochugirl-blog]

HPLG Update

I just wanted to give you all a little warning that this coming chapter starts off with a bang and it’s dark. The reading rating for this chapter does raise the story from T to OT/NC-17. I will be posting two versions of the chapter, one in which goes into detail where the other is mentioned. The chapter is now about half way done. I do apologize for the delay I have been recovering from surgery, I had hoped to have it done and out before my surgery but sadly timing wasn’t my friend. 

Fanfiction.net will be getting the lighter version while Archive of our Own will be getting the darker. I will be posting both here on Tumblr.

[konekochugirl-blog]

Explanation to events in Chapter 5 of HPLG

Chapter can be read Here.

The reason I put Alexa in such a situation is cause, I’ve been working though a lot of stuff. Without giving too much of her journey away, I am hoping to make her a role model for girls going through something like that. I do apologize if it’s too troubling and wish to drop my story after what happened, there is no excuse for anyone to do that to anyone. I don’t condone it, I am a survivor too. I’m simply making a role model I wish I had when I needed one.