#polylink

[randompolykin]

poll for poly kins, therians, links kiths, and the likes

please feel free to add details in comments or reblogs!

[theverynothumankai]

I n T r O d U c T i O n P o S t

I’m Kai, also called Malakai. This is my blog for all things Nonhuman. Come join my little side of tumblr. <3

I’m a Polytherian, Polykin, Polylink, Otherfix, Otherhearted, and Fictionflicker/Otherflicker.

Theriotypes: Vancouver Coastal Sea Wolf (also known as Coastal Wolf and Vancouver Island Wolf); Canada Lynx; Red Fox; Nurse Shark; Snow Leopard; Maned Wolf; Norwegian Forest Cat; Coyote; Side-Striped Jackal; Dhole. Kintypes: Dragon (SeaWing/SilkWing Hybrid); Dragon (Night-Light Fury Hybrid); Dark Fey (Forest Type, from Maleficent); Avian (winged humans with tails); Raccoon (gold winged and gold horned); Silver Fox (with big crow-like/themed wings); Angel (shape-shifting, three pairs of wings, comforter of those who are lost). Hearttypes: Whale Shark. Flickertypes: Hylian (from TOTK/BOTW); Orphinki (OC species); Sable; Owl (all). Questioning: Ocelot Theriotype; Manta Ray Theriotype; Orca Whale Theriotype; Maine Coon Cat Theriotype; Jumping Spider Theriotype or Hearttype; Pallas Cat Theriotype; Manta Ray Kintype; Siren Kintype; Dragon Kintype (feathered wings); Mosasaur Theriotype; Blue Whale Theriotype; (Baby) Bongo Theriotype; Kirk’s Dik Dik Theriotype.

More Info On My ‘Types Here.

I’m a PseudoHuman, which means I may appear to be human, but am not.

I’m SunNonhuman, which means my nonhumanity is integrated into my baseline personality. It does not prevent me from having minor fluctuations of mood that feel slightly more animal or slightly less animal. I do not experience mental shifts to my nonhuman types.  I’m Transspecies (more specifically Humanfluid) and Voidpunk, which means I identify as another species (and more specifically have a fluctuating connection to my humanity), and that I’m reclaiming the dehumanization and demonization that I face in the marginalized minorities I’m in, basically I’m reclaiming dehumanization and fully embracing inhumanity/nonhumanity.

I’m Physical Nonhuman, in the way that since I am my ‘types, so is my body (although, I am aware that my body is technically human/human-presenting).

I’m a CladoNonhuman, specifically with my Owl Flickertype. I am all owls, not just one or a few, although I am more so connected to a couple specific species rather than equally to all. My Owl Flickertype comes and goes, it’s been gone for a while but it’s coming back again, and I have no doubt that that will happen again.

My Otherfix identity influences my nonhuman identity overall, by the way! So, a lot of my theriotypes and kintypes are due to hyperfixations (although, remember, they are involuntary and permanent for me!), and then a lot of my flickertypes have been due to hyperfixations (once again, involuntary and the flickertypes come and go).

The reasons/origins of my Nonhumanity is due to a combination of Past Lives, Fate, and Neurodivergency. I believe I was all my ‘types in past lives, and that I only found out about being these things in this life due to my neurodivergency.

I fluctuate between Nonhuman and Human, although am always on some level both, just other times are stronger for one and the other not so much, but overall I mostly refer to myself as just Nonhuman. My soul is a combination of all my ‘types, plus the intruding human soul of this life.

Please only refer to me as Nonhuman and Alterbeing, NOT as Human or Alterhuman.

I do not experience phantom shifts or any sort of shifts, but when I have in the past, it has only been once, very short (a few seconds), and partial. I experience loads of species dysphoria, envy, and sometimes euphoria, though.

I only have very poor gear, two homemade tails, and one mask, and am also currently working on wings. I’m hoping to get more materials to make better masks and tails for myself soon, though.

I do very poor quadrobics, but I adore doing them, especially when I can in public.

Fellow Physical Nonhumans/Alterbeings/Alterhumans, Non-Spiritual Nonhumans/Alterbeings/Alterhumans, and etc. are very welcome here.

I’m very queer. My gender is way too complex, but just imagine it as masculine, genderless, and animalistic, with lots of different aesthetics.

I use He/It/Rot/Void/Mutt/Pup/Teeth/Woof/Bone/Claw/Plant/🐾/🐺/🦝/🌿 pronouns + many more with those themes.

I’m AroAce, Aromantic and Myrsexual (Neuroasexual, Nebulasexual, Merosexual, Placiosexual, Dreadsexual, Acorsexual, Apothisexual, Bellussexual, Rosesexual, Requisexual, and Falsaesthsexual).

I’m Homoalterous (Gay/MLM), Omniqueerplatonic (preference for non-men), Panexteramo, Pansensual, and Panaesthetic.

I’m Ambiamorous with no preference. I’m heavy T4T-leaning. I’m also a Lesboy.

I’m undiagnosed/self-diagnosed (with research)(in the process of getting this all diagnosed) AuDHD, Pure O OCD, APD, SPD, RSD, BDD, ARFID, SAD, PTSD, Trichotillomania, Misophonia, and Dermatillomania.

I’m diagnosed MDD and GAD.

I’m undiagnosed (and in the process of getting it diagnosed as well) PMDD and/or PME.

SH and SI survivor, and in recovery.

I struggle with severe violent and ugly intrusive thoughts (due to my OCD), and have for years. Not as bad as it used to be, but still have some at least once a day.

I consider myself disabled due to my severe executive dysfunction that I’ve had for years, as well as for other reasons.

I also have severe imposter syndrome that I deal with 24/7.

I have special interests (Wings Of Fire, Harry Potter (I really love the marauders), Dragons, and Neurodivergency + Disabilities + Disorders), hyperfixations (current ones are nonhumanity and nonhuman gear), verbal shutdowns, shutdowns and meltdowns, am often in burnout, and mask quite a lot.

I’m in therapy, go every week, and love my therapist so much. /gen

Please use tone tags/indicators when talking to me. They are greatly helpful and appreciated. Thank you!

I’m taken by my lovely long distance mate, @itssiryapsalot @theremixedcreature.

I’m a minor (16-years-old).

I’m Agnostic, spiritual, and do not follow any God or Gods. I believe in past lives, fate, soulmates, twin flames, manifestation, the multiverse, the paranormal, witchcraft, and much more.

I’m a Reality Shifter and have shifted twice to parallel realities. (I’m a safe places for permashifters, respawners, and those who shift to realities where they’re a different race.)

I have one sibling (She/Her), @belles17 @fuckthisclubup. I love her loads, and yes, she is aware that I’m Nonhuman (and is totally chill with it).

My other Nonhuman accounts are: @sirius-the-lonely-angel and @malakaitheyappingwolf.

My Nonhuman Pinterest account is here.

More about me here.

I don’t feel like making a DNI, but my block button is my best friend, so get ready. Obviously, no anti-alterhumans, anti-furries, queerphobes, radqueers and radfems, TERFs, SWERFs, offending MAPs & Paraphiles & etc., sexists, racists, ableists (including those believe in “narcissistic abuse”), and etc. are allowed here. Also, those who are endo/non-traumagenic/etc. systems are safe here, so those against them can leave please.

Full credit to the original creators/owners of the dividers!!!

[theverynothumankai]

1/ i’m a polytherian, polykin, polylink, otherfix, otherhearted, and fictionflicker/otherflicker!

2/ vancouver coastal sea wolf, snow leopard, canada lynx, nurse shark, red fox, dragon (silkwing/seawing hybrid), dragon (night-light fury hybrid), dark fey (forest type, from maleficent), raccoon (gold winged and gold horned), owl (all), sable, hylian, orphinki (oc species)

3/ nope! :/

4/ hmmm, often experience dysphoria at not being recognized as nonhuman, at not having paws, claws, fur, but it overall fluctuates since i’m humanfluid and experience my nonhumanity more casually a lot of the time

5/ it has its ups and downs. tumblr is much better than any other platform, but still there’s sm discourse and shit instead of us just living and being.

6/ when fellows recognize and refer to me as my ‘types, when i’m able to do quads, when i have food that i crave, and much more

7/ yes, a lot of the time i am

8/ don’t be afraid to identify as yourself, to go for ‘types, to say what you are, to be comfy with yourself, even if you’re unsure about it all still, everyone can and will be wrong from time to time but as long as you aren’t purposefully misidentifying as someone and using it as a way to harm others, then it’s all good and just go for it, even if you find out you’re wrong later on, you’re awesome and it’s a journey yk

9/ i really want gear but likely won’t get any till much later in life due to my situation

10/ my nonhumanity is due to past lives, fate, and neurodivergency, i believe i was all my ‘types in past lives, but only discovered it all in this life due to my neurodivergency

11/ @feralp4ws @sawyeryaps

If you are a alterhuman, reblog and answer these questions!

(don't be afraid to write a lot, do what you want ¯⁠\⁠_⁠(⁠ツ⁠)⁠_⁠/⁠¯)

1/ Which category of alterhumanity do you belong to?

2/ What/who is/are your type(s)? (if you have any)

3/ Do you experience shifts? If so, can you tell us your most common shifts and your strangest cameo shift (if you've ever had a cameo shift)?

4/ How do you experience your alterhumanity in everyday life?

5/ What do you think of the community?

6/ What are the things that make you most comfortable and euphoric in your alterhumanity?

7/ Are you experiencing species dysphoria?

8/ What advice would you like to say to a young alterhuman who has just awakened?

9/ Do you have/want to have gears?

10/ Do you know/have any theories about the origin of your alterhumanity? If so, tell us! (all beliefs are legitimate)

11/ Tag someone/a creature to answer these questions!ㅤᵕ̈

[blockchainnewsmeuae]

Exploring the Concept of a Polygon Bridge: What Does It Do?

Unlock the future with PolyLink - your gateway to the world of Polygon Bridges. Explore what they do, how they can revolutionize your digital life, and embark on a journey to a seamless, decentralized future.

[thevoidz-blog]

Does anyone else otherkin or otherlinks contradicte yourself? Like I feel more comfortable in the winter and it feels more close to my kintype.

But also

I hate snow. Especially if I have to go out.

It's pretty and I love playing in it for like 5 seconds but then I want to go inside and cuddle up in blankets.

I like winter if I'm inside but once I'm outside it could be better.

I mean who knows maybe I'm starting to like it. .

I just only hate it cause I have to walk in it in the morning for school.

And that sucks

[luxxeonthings]

Tetra Polylink (Orderly Tangle) | Blender 3d Motion Graphics | 3d Animation

This is an Orderly Tangle of six equilateral triangles interlocked in a polylink.  This animated render was created in Blender 2.8 using the Eevee render engine.  If you're interested in this model for 3d printing or rendering, you can find it at the links below:

  https://www.cgtrader.com/luxxeon

https://gumroad.com/products/dKlTG

[otiososmanus]

It was Paper Polylinks by George W. Hart

that I first made reference to on the start of this journey.

And Hart provided a host of way markers throughout.

[everythingaboutbiotech]

E.Coli Expression Vector

The gene to be expressed is inserted into one of the restriction sites in the polylinker, near the promoter (P), with the end encoding the amino terminus proximal to the promoter. The promoter allows efficient transcription of the inserted gene, and the transcription termination sequence sometimes improves the amount and stability of the mRNA produced. The operator (O) permits regulation by means of a repressor that binds to it. The ribosome binding site provides sequence signals needed for efficient translation of the mRNA derived from the gene. The selectable marker allows the selection of cells containing recombinant DNA.

[stratviewresearch]

Cross-Linked Polyethylene Market is Projected to Show Profitable Expansions Over the forecast period 2022-2028

Cross-Linked Polyethylene Market research report serves as one of the best medium to help startups in business planning. It brings all the relevant data, which provides insights into consumers’ buying nature, location and thinking pattern. Keeping an eye on which actions are followed by the competitors and their future business plans for the estimation period 2022-2028 is easy by referring this report. Monitoring present and upcoming market trends is possible with this Cross-Linked Polyethylene Market report. High-quality and effective market research report has the potent to minimize business risks which can create problems in making marketing related decision. 

Download Sample Report PDF (Including all Table & Figures), here –

Cross-Linked Polyethylene Market report guides how, when and where to expand the business by making right investment. Potential competitors are identified here along with allow business players to increase the product portfolio. Customers’ wants and a clear view on future business growth are provided in this Cross-Linked Polyethylene Market study report. Right investment in the product development has great importance in the increase of product sales. It also allows attaining the comprehensive understanding of how customers perceive products. It also reveals how their requirements are shaped as well as influenced and how they choose the product launch on the basis of them. Novel product launch ideas provided here work as the perfect guide for the key participants to augment the product sales.

Key Players

Key players mentioned in the report are as follows -

Borealis AG

Charloma Inc.

3H Vinacom Co., Ltd.

Solvay S.A

Falcone Specialties AG

HDC Hyundai EP

Hanwha Chemical Corporation

Exxonmobil Corporation

Polylink Polymers (India) Ltd.

Zhejiang Wanma Macromolecule Material Co., Ltd.

Growth drivers and Market Value:

This report, from Stratview Research, studies the Cross-Linked Polyethylene Market value and growth drivers over the trend period of 2022-27. According to the report -

Cross-Linked Polyethylene Market is likely to witness an impressive CAGR of 8.2% during the forecast period. The major drivers contributing to the growth of the PEX market are the automotive and the construction industries in APAC, mainly in India, China, Japan, South Korea, and the Southeast Asian countries.

Segment Analysis:

Based on Type:

Based on the type, the market is segmented as High-Density Polyethylene (HDPE), Low-Density Polyethylene (LDPE), and others. The HDPE segment is estimated to lead the market during the forecast period. This growth is attributed to the remarkable combination of properties, which enable HDPE as the ideal material for diverse applications in various industries. The growing demand for products manufactured by using HDPE, owing to the high growth in the construction industry and improved investments in the industrial and public infrastructure in the developing regions of APAC, Europe, North America, South America, and the Middle East & Africa is likely to boost the demand for PEX during the forecast period.

Based on Region:

In terms of regions, Asia-Pacific is estimated to be the largest and fastest-growing PEX market during the forecast period. The market is driven by the region’s rapid industrialization and expanding transmission & distribution lines in the electrical & communication industry, thereby, bolstering the demand for PEX-based wires & cables. Further, the extensive use of XLPE wires in automotive battery cables, wire & cable coating layer, motors, and other high-voltage resistance materials in the Asia-Pacific region is also boosting the PEX market. In addition, the rising residential and non-residential infrastructures in this region are contributing to the construction of pipelines and tubing, which is underpinning the PEX market in the plumbing application. North America and Europe are also expected to offer substantial growth opportunities in the coming five years.

Know more about the report, click here

Cross-Linked Polyethylene Market research report also has the potent to bring overlooked and abandoned consumers back into focus by covering all the important market and customer data. It further assists to raise the business success rates. It spots out consumer requirements and augments the future engagements of the firm with them. Important findings are covered in this Cross-Linked Polyethylene Market study report to help newly emerging industry players to establish their business in the cut-throat market. Studying customer behavior is significant as it assists them factors that can change customers’ buying decision making. By having complete understanding of how customers decide on particular product or service launch, central participants are able to spot out the products and fill in the gap which are required and the products that are outdated. This Cross-Linked Polyethylene Market report permits novice players to have best understanding regarding target market and create products, which are going to fascinate novel customers, sell and develop brand loyalty. Engaging customers is one of the leading factors to attain precise and actionable insights, which drive the rapid product development. All the customer related factors including their motivations, inclinations and buying nature are all covered here.

 About us –

Stratview Research is a global market research firm that offers reliable market reports, market entry strategies, strategic growth consulting, and more. The market experts compile high-quality market information to help users obtain granular level clarity on current business trends and expected future developments. Stratview Research also offers customisation of the reports. Reach out to the analysts to customize the given report according to your priority/requirement.

Stratview Research has also launched Composights, an online portal that offers free thought leadership reports, whitepapers, market report synopsis, and much more for Composites and allied industries, worth US$ 20,000 every year.

Click here to sign up (No costs involved): https://www.stratviewresearch.com/composights/sign-in

Connect with the team at –

Stratview Research

E-mail: sales@stratviewresearch.com

Direct: +1-313-307-4176

[sbsgenetech]

We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units. Please do not be limited by restriction sites and polylinkers , we will synthesize the various gene sequences you need.

[ashwinigongale-blog]

Global Cross-Linked Polyethylene Market Trend, Marketing Channels, Major Industry Participants, Strategies  and Forecast To 2024

A new research report data added to the Market.biz titled Global Cross-Linked Polyethylene Market gives a proper understanding of the industry and it also predicts current market trends and future market trends. The report presents a detailed picture of the market by way of study, synthesis, and addition of data from multiple sources. Global Cross-Linked Polyethylene market analyses the report based on customer demand, supply and demand status, competitive market scenario and industry policies.

Here, you can avail Sample Copy for Global Cross-Linked Polyethylene Market Report

The Cross-Linked Polyethylene market report presents the overall structure of the market like the major market players based on the company profile, sales revenue, the business tactics used in the global Cross-Linked Polyethylene market which will help the emerging market segments in making vital business decisions. Also, the report discusses business plans, sales and profit, market stations and market volume, raw material suppliers, and buyers demand information and distribution ratio.

Competitive landscape

The competitive landscape segment in the report relies on the current as well as the potential of the leading market players in the industry. It offers the in-depth company profiles, that may stay active in predestined decades, combined with company profile, product launches, product market, and gross margin, financial details, key advancements, business short-term and long-term marketing, and advertising strategies and SWOT analysis of these competitive firms.

Cross-Linked Polyethylene Market: Top Companies

Borealis AG

3H Vinacom Co. Ltd.

Saco Polymers Inc.

Falcone Specialities AG

Akzonoble N.V.

Kkalpana Group

Arkema Group

Hanwha Chemical

Sankhla Polymers Private Limited

Exxonmobil Chemical Company Inc.

Vanderbilt Chemicals LLC

Polylink Polymers (

 Market Segmentation

The report provides global market segmentation based on the product type, end-users, and region. In the additional section, the report includes market division study over the significant geographies of the world such as North America, Asia-Pacific, Europe, South America, The Middle East & Africa and the rest of the world. The report covers worldwide statistic which is focused on the global market.

Cross-Linked Polyethylene Product types Outlook:

High Density Polyethylene (HDPE)

Low Density Polyethylene (LDPE)

 Cross-Linked Polyethylene Applications Outlook:

Plumbing

Wires & Cables

Automotive

 Geographically, the key segments of the market are:

North America

Europe

China

Japan

The Middle East & Africa

India

South America

Make an Inquiry to Get Global Cross-Linked Polyethylene report as your requirement

Global Cross-Linked Polyethylene Market Report mainly covers the following significant points:

1. An outlook of the Cross-Linked Polyethylene industry;

2. Market Competitive Landscape;

3. Top Players Company Profiles;

4. Analysis of the product types of Cross-Linked Polyethylene;

5. Analysis of the Applications/End-Users of Cross-Linked Polyethylene;

From the conclusion of this study, all the answers to the queries will be well guided as it focuses on analytical research and mainly includes qualitative and quantitative analysis. this analyzed report can give an in-depth summary of upcoming and ongoing which leads to market development on a global scale.

Click Below If You Want More Related Reports:

Global Progesterone Market

Global Automatic Underwater Vehicle (Auv) Market

Global Non-Fused Disconnect Switch Market

Global Crude Oil Desalter Equipment Market

[eleanorening-blog]

Cross-Linked Polyethylene Market Share by 2025: QY Research

In its recently published report, QY Research has provided unique insights about global Cross-Linked Polyethylene market for the given period. One of the main objectives of this report is to categorize the various dynamics of the market and to offer latest updates such as mergers and acquisitions, various technological developments, new entrants in the market, which make an impact on different segments of the global Cross-Linked Polyethylene market.

Request a Complete Sample of this report at http://www.qyresearchglobal.com/goods-1858783.html

The scope of the global Cross-Linked Polyethylene market:

For the better understanding of the market, this report has provided a detailed analysis of trends, drivers and restraints that dominate the present market scenario and also the future status of the global Cross-Linked Polyethylene market during the projected period of 2018-2025.  The report is segmented this market on the basis of regions, (segment name) and (segment name).

While classifying these segments, the expert team of analysts have listed down the relative contribution of each segment for the growth of global Cross-Linked Polyethylene market. Detail information of segments is required to recognize the key trends influencing the global market for Self Contained Breathing Apparatus (SCBA).

Each segment of the market provides an in-depth information on the qualitative and quantitative aspect of the market. While giving a brief idea about the revenue opportunities for all the segments, this report has also provided the value of absolute dollar opportunity for all the segments over the predicted period of 2018-2025.

Crankshaft Tortional Vibration Damper Market: Key Players

   DOW Chemical Company

   Akzo Noble N.V.

   Arkema Group

   Borealis AG

   Lyondellbasell Industries N.V.

   Exxonmobil Chemical Company, Inc

   3H Vinacom Co., Ltd.

   Solvay SA

   Hanwha Chemical

   Polyone Corporation

   Hyundai EP

   Kkalpana Group

   Kanoo Group

   Polylink Polymers (India) Ltd.

   Saco Polymers Inc.

   Sankhla Polymers Private Limited

   Silon S.R.O.

   Falcone Specialities AG

   Vanderbilt Chemicals LLC

Crankshaft Tortional Vibration Damper Market: Segments

   Plumbing

   Wires & Cables

   Automotive

Crankshaft Tortional Vibration Damper market regional Analysis

The global Cross-Linked Polyethylene market is spread across the globe which not only includes the market of North America but covers the other regions such as Europe, the Asia Pacific, the Middle East, and Africa and the rest of the world.

North American countries, especially the US and Canada represents remarkable growth in this market. In a similar way, Western European regions are also ahead in influencing the global markets. Increasing disposable income, ever-changing lifestyle and high economic growth are some of the factors which are responsible for the growth of global Cross-Linked Polyethylene market in North America and Europe region.

The report is committed to offer in-depth market analysis

QY Research is committed to offer an accurate and precise data to the readers. With the help of base numbers and segments, the basic market information has been formulated and is triangulated based on various verticals by taking into consideration demand and supply-side drivers and few other dynamics of regional markets.

Apart from this, QY Research has also presented an index for market attractiveness for the better understanding of various segments. One of the major reasons behind offering market attractiveness index is to assist the clients and target audience to recognize the several market opportunities in the global Cross-Linked Polyethylene market.

Report on Cross-Linked Polyethylene market mainly covers the 15 sections

Chapter 1 describes the global Cross-Linked Polyethylene market introduction, scope, market overview, market risk, and market driving force

Chapter 2 highlights the competitive situation among the top players with market share, revenue and sales in the Cross-Linked Polyethylene market in 2018-2025

Chapter 3 shows the global Cross-Linked Polyethylene market by regions, with market share, revenue, and sales of Cross-Linked Polyethylene market for each region, from 2018 to 2025

Chapter 4 shows the global Cross-Linked Polyethylene market by type and application, with sales revenue, market share and growth rate by type, application, from 2018-2025

Chapter 5,6,7,8 analyzes the major regions, with sales, market share, and revenue of Cross-Linked Polyethylene market by key countries in these regions

Chapter 9,10,11,12 includes global Cross-Linked Polyethylene market forecast, by type, application and by regions, with revenue and sales, from 2018 to 2025

Chapter 13, 14 and 15 describe Cross-Linked Polyethylene market sales channel. Distributors, traders, data source, appendix, and research and findings

Contact US

QY Research, INC.

Tina

17890 Castleton, Suite 218,

City of industry, CA – 91748

USA: +1 626 295 2442

Email: sales@qyresearchglobal.com

Web: www.qyresearchglobal.com

[tomaslcoston]

PolyLinks renamed Enplas Life Tech

Life sciences molding specialist PolyLinks Inc. has changed its name to Enplas Life Tech Inc. following its acquisition by parent company Enplas Corp. From a great article at Plastics News -Medical - RSS News Feed http://www.plasticsnews.com/article/20180118/NEWS/180119912/polylinks-renamed-enplas-life-tech

[luxxeonthings]

Solid, manifold topology in compliance with 3d Printing rules and standards.

Blender 2.81 scene ready to render with Eevee materials and settings.

3dsmax 2017 with low poly base mesh and OpenSubDiv modifier

3dsmax scene ready to render with VrayNEXT materials

UV-Unwrapped with UVW Mapping.

Subdivided OBJ included for import into any 3d package.

STL file included for 3d printing (SLS printing recommended)

[otiososmanus]

So this was the first orderly tangle construction I made during the covid lockdown. It's the Four Triangle Polylink. (Actually, this is one of two types of four triangle polylink - the better known one.) As this polylink is thought of as being derived from the tetrahedron, that Platonic solid is considered the structure’s “parental polyhedron.”

[audiopedia2016]

What is CLONING VECTOR? What does CLONING VECTOR mean? CLONING VECTOR meaning - CLONING VECTOR definition - CLONING VECTOR explanation. Source: Wikipedia.org article, adapted under http://ift.tt/yjiNZw license. A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme that creates the same overhang, then ligating the fragments together. After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use. There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages (such as phage ?), cosmids, and bacterial artificial chromosomes (BACs). Some DNA, however, cannot be stably maintained in E. coli, for example very large DNA fragments, and other organisms such as yeast may be used. Cloning vectors in yeast include yeast artificial chromosomes (YACs). All commonly used cloning vectors in molecular biology have key features necessary for their function, such as a suitable cloning site and selectable marker. Others may have additional features specific to their use. For reason of ease and convenience, cloning is often performed using E. coli. Thus, the cloning vectors used often have elements necessary for their propagation and maintenance in E. coli, such as a functional origin of replication (ori). The ColE1 origin of replication is found in many plasmids. Some vectors also include elements that allow them to be maintained in another organism in addition to E. coli, and these vectors are called shuttle vector. All cloning vectors have features that allow a gene to be conveniently inserted into the vector or removed from it. This may be a multiple cloning site (MCS) or polylinker, which contains many unique restriction sites. The restriction sites in the MCS are first cleaved by restriction enzymes, and a PCR-amplified target gene, also digested with the same enzymes, is then ligated into the vectors using DNA ligase. The target DNA sequence can be inserted into the vector in a specific direction if so desired. The restriction sites may be further used for sub-cloning into another vector if necessary. Other cloning vectors may use topoisomerase instead of ligase and cloning may be done more rapidly without the need for restriction digest of the vector or insert. In this TOPO cloning method a linearized vector is activated by attaching topoisomerase I to its ends, and this "TOPO-activated" vector may then accept a PCR product by ligating both the 5' ends of the PCR product, releasing the topoisomerase and forming a circular vector in the process. Another method of cloning without the use of DNA digest and ligase is by DNA recombination, for example as used in the Gateway cloning system. The gene, once cloned into the cloning vector (called entry clone in this method), may be conveniently introduced into a variety of expression vectors by recombination.