#Next-Generation Sequencing

[gauricmi]

Maximizing Efficiency: Best Practices for Using Sequencing Consumables

By implementing these best practices, researchers can streamline sequencing workflows, increase throughput, and achieve more consistent and reproducible results in genetic research. Sequencing Consumables play a crucial role in genetic research, facilitating the preparation, sequencing, and analysis of DNA samples. To achieve optimal results and maximize efficiency in sequencing workflows, it's essential to implement best practices for using these consumables effectively.

Proper planning and organization are essential for maximizing efficiency when using Sequencing Consumables. Before starting a sequencing experiment, take the time to carefully plan out the workflow, including sample preparation, library construction, sequencing runs, and data analysis. Ensure that all necessary consumables, reagents, and equipment are readily available and properly labeled to minimize disruptions and delays during the experiment.

Optimizing sample preparation workflows is critical for maximizing efficiency in sequencing experiments. When working with Sequencing Consumables for sample preparation, follow manufacturer protocols and recommendations closely to ensure consistent and reproducible results. Use high-quality consumables and reagents, and perform regular quality control checks to monitor the performance of the workflow and identify any potential issues early on.

Utilizing automation technologies can significantly increase efficiency when working with Sequencing Consumables. Automated sample preparation systems and liquid handling robots can streamline repetitive tasks, reduce human error, and increase throughput. By automating sample processing and library construction workflows, researchers can save time and resources while improving consistency and reproducibility in sequencing experiments.

Get More Insights On This Topic:  Sequencing Consumables

[simplyforensic]

Sequencing: A Deep Dive into the History of DNA Techniques

Sequencing: A Deep Dive into the History of DNA Techniques The evolution of DNA sequencing technologies marks a fascinating journey of scientific breakthroughs, from the early days of manual, labor-intensive methods to the current era of high-throughput, automated systems. This history can be divided into three main generational shifts, each characterized by significant advancements in…

[tekmaticinc]

Tekmatic: Next-Generation Sequencing | Explore the Technology

Next-generation sequencing (NGS) describes high-throughput methods that can swiftly determine the sequence of a specific nucleic acid strand, such as DNA or RNA. How we perform genomics research has altered as a result of these tools. There are numerous methods that use different operating principles under the umbrella term "next-generation sequencing."

[bisresearchreports]

Liquid biopsy is a non-invasive medical procedure which holds the ability to detect the presence of molecular cancer biomarkers in biological fluids. Liquid biopsy has a critical role in the precision medicine approach, as it confirms the safe and effective application of targeted therapeutics. Significant increase in the research and development activities pertaining to next-generation sequencing are underway to develop NGS products and services, which are expected to increase due to the rising number of chronic disease burdens such as cancer

[luck-of-the-drawings]

THIS THING IS SCUUUFFED AS HELL & ITS ALSO THE BEST THING I HAVE ANIMATED THUS FAR. IM SO IN LOVE WITH EMIZEL. JUST WISH I GAVE HIM MORE STUPID TATTOOS. NEXT TIME THO. NEXT TIME. I ALSO LOVE VEX&VIV SOOOO MUCH. charlies flavor of Deranged is my FAVORITE!!

[medi-techinsights]

Global Next-generation Sequencing Market valued at $6.3 billion (2021), is set to witness a lucrative growth of 16%

Next-generation sequencing is a technique for determining the sequence of DNA or RNA in order to investigate genetic variations linked to diseases or other biological phenomena. NGS refers to a set of technologies that use massively parallel sequencing to generate millions of short read sequences in a shorter time and with considerably higher throughput.

Recent developments in sequencing platforms, increasing applications of NGS, rising demand for NGS services, development of advanced bioinformatics tools and growing incidence of cancer and infectious diseases are the key factors driving the growth of the global next-generation sequencing market.

Technological advancements in Sequencing Platforms

Continuous advances in sequencing technology have enabled to develop efficient, portable, and easy-to-use NGS sequencers to deliver rapid and precise results while also reducing turnaround times. Such advancements tend to provide competitive edge to manufacturers and therefore, major players are continuously focusing on investing in research activities for new product development to strengthen their positions in this high growth market. Some of the technological advancements are listed below:

In March 2022, Element Biosciences Inc. launched its benchtop sequencer - Element AVITI System, that offers combination of performance, cost, and flexibility in an unprecedented way. This system consists of a benchtop NGS instrument and related consumables.

In December 2021, Singular Genomics Systems, Inc. commercially launched its G4 platform, a powerful benchtop sequencer featuring a new high-performance chemistry with advanced engineering to give precision, flexibility, speed, and power for a wide range of applications, including cancer and immunology research.

Partnership and collaboration to boost adoption and research outcomes for diagnostic applications

The next-generation sequencing market has witnessed a number of partnerships and collaborations by key market players to enhance the adoption of this technology for clinical applications and co-develop advanced diagnostic solutions. The strategic initiatives that boost the adoption of NGS for diagnostic applications are listed below:

In January 2022, Illumina entered into multi-year partnership with Agendia N.V., a precision oncology provider for breast cancer, to co-develop in vitro diagnostic (IVD) tests for oncology testing using NGS technology.

In July 2021, Qiagen partnered with Sysmex for the development and marketing of cancer companion diagnostic (CDx) solutions, leveraging Sysmex’s Plasma-Safe-SeqS technology for NGS.

In April 2021, Thermo Fisher Scientific collaborated with Mayo Clinic, a non-profit organisation to accelerate access to innovative diagnostic solutions for haematology, allergy, oncology, and autoimmunity diagnostics, leveraging NGS technology.

Competitive Landscape Analysis: Next-Generation Sequencing Market

The global next-generation sequencing market is marked by the presence of leading market players such as Illumina (US), Thermo Fisher Scientific (US), Pacific Biosciences (US), Oxford Nanopore Technologies (UK), Hoffmann-La Roche AG (Switzerland), QIAGEN (Germany), and others.

Get Customized Report on Next-Generation Sequencing Market @ https://meditechinsights.com/next-generation-sequencing-ngs-market/

[the-heart-of-a-monster]

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[liquidstar]

a friend who'd wait :)

[simplyforensic]

The Dawn of Next Generation Sequencing Technologies: A Revolution in Genomic Science

In genomic science, the advent of Next-Generation Sequencing (NGS) technologies has marked a transformative era, radically changing our approach to understanding the complexities of life at a molecular level. NGS, also known as high-throughput sequencing, encompasses several advanced methodologies that enable rapid sequencing of DNA and RNA much more swiftly and cost-effectively than…

[tekmaticinc]

Next-Generation Sequencing | Explore the Technology - Tekmatic 

The term "next-generation sequencing" (NGS) refers to high-throughput technologies that can quickly ascertain the sequence of a particular nucleic acid strand, such as DNA or RNA. These tools have changed how we conduct genomics research. "Next-generation sequencing" refers to various technologies that operate according to various operating principles.

[bisresearchreports]

Cardiovascular diseases, or CVDs, are one of the leading causes of death globally. As per a study from WHO, this number is roughly 32% of all global deaths.  This rising prevalence of CVDs has pressed physicians and scientists to look for new and advanced ways of diagnosing cardiovascular diseases.

Precision cardiology has emerged as one such medical technology that is helping in transforming the overall healthcare system for managing CVDs.

[cbirt]

Scientists from the University of Cambridge have introduced a cutting-edge DNA sequencing method that can precisely pinpoint the location and mechanism of small molecule drugs interacting with the targeted genome. The novel technique has the potential to provide valuable insights into the drug development process and increase our understanding of the complex interactions between drugs and the genome.

Understanding how drugs interact with cells is crucial to developing new and effective therapies. A critical aspect of this process is understanding how drugs bind to chromatin, which is composed of DNA and proteins and plays a pivotal role in regulating gene expression. Chromatin is also where drugs can bind to and interact with the cell. A game-changing technique Chem-map, introduced by the researchers, sheds light on the previously mysterious interactions between small molecules and their targets on the DNA genome. In this article, let’s explore the role of Chem-map in profiling drug binding to chromatin in cells.

Continue Reading

[shatterstar]

If you see me on Spotify listening to rush limelight 45 times in a row don’t worry about it

[sshbpodcast]

Wake me up before you boldly go go: Dreams in Star Trek

By Ames

I’m feeling pretty tired at the moment, specifically tired of dream sequences in various media. They’re a fairly overused trope: seeing some slightly off scenes that either teach our protagonists something they need to know or just toy with the audience to make them believe something is real. Until the characters wake up. Even Star Trek, a show allegedly bounded by science, falls victim to the lazy writing and overused device of the dream episode.

That’s not to say there aren’t some good dream episodes out there, and even in here. So this week A Star to Steer Her By is analyzing some dreams, some nightmares, anything that we might find in the subconscious and wonder if it means anything (even if it’s just to the plot). So get tucked in, snuggle up with Kukalaka, and drift out of consciousness below and/or on this week’s podcast episode (discussion starts at 1:15:29). Let’s see if all of this was just a dream.

Somehow, we couldn’t think of any episodes from The Original Series or The Animated Series that had dream sequences, which is a little funny since you’d think the campier and arguably less cerebral shows would have some true nightmares. But we’re starting off with a whole slew of episodes from The Next Generation instead.

[images © CBS/Paramount]

“Shades of Grey”

Coma dreams are their own subsection of dream episodes, but definitely worth including in our list because they present some interesting examples of storytelling and structure. And then there’s “Shades of Grey.” As far as clipshows go, it’s got an okay wrapper in the form of some Riker dream manipulation, but it has too much going against it to be any good overall.

“Night Terrors”

Mention episodes that aren’t any good and “Night Terrors” will arise from your subconscious. As far as dream plots go, it’s actually got a decent concept in depriving everyone but Troi of REM sleep, so there are some really surreal (if disjointed) scenes, but watching what they put Marina Sirtis through proved to be the real nightmare.

“Violations”

This one’s probably in that grey area between visions and coma dreams, but worth mentioning just to bring up what a messed up thing that bastard Jev does to people – putting skewed, traumatic memories in their heads that leaves them in comas. Like in “Night Terrors,” it’s got some really upsetting scenework and direction that leaves you feeling appropriately disturbed.

“The Inner Light”

One of the best episodes of all of Star Trek is effectively a coma dream that Picard has in which he lives the life of Kamin in the twenty or so minutes that he’s knocked out by the Kataan probe. Talk about great use of the dream structure! We don’t need to deal with surreal nonsense because of the programming, and the effects on Jean-Luc are personal and emotional.

“Tapestry” 

This one is debatable based on your interpretation. On the podcast, Jake likes to spread the possibility that none of this episode happened at all and it was all a dream Picard had while on Dr. Crusher’s surgical table. Which is an interesting way to think about this genuinely thought-provoking episode, although we enjoy the other view that Q’s behind it all as well.

“Birthright”

After a certain visiting doctor zaps him in the face, Data is able to unlock his dreaming program that daddy Soong had left for him as a little present. And it’s all pretty on-the-nose kinda stuff that dream episodes can be guilty of. Every little action is meaningful in some way that is just utterly unrealistic, but Data’s also an android, so realism may already be out of the question.

“Frame of Mind”

I’d say “Frame of Mind” might be our first real example of a good traditional dream episode. It’s not just a story told in a dream or a technique for the writers to convey something without stating it. This Riker mind adventure is meant to be a disorienting experience specifically because it’s all in his head. There was method to this madness and it paid off in spades!

“Phantasms”

This infamous episode is the reason why I wanted to do this post. “Phantasms” commits pretty much every sin a dream sequence can commit: it mainly uses characters from the episode in absurd roles, the dream makes too much sense in world because everything in it correlates to something from real life, and its randomness and surrealness just come across as a formulaic writing device. With mint frosting.

“Dark Page”

Literally on the heels of “Phantasms” is what I’d call a more interesting and effective use of the dream, in this case the coma dream that Deanna visits inside Lwaxana’s head. The symbolism isn’t as overt and at the same time there’s more emotional connection with the scenelettes. I also give it a pass because Betazoid mind powers help this one from feeling too heavy handed.

First Contact

Somehow, we don’t see a guilt-driven dream sequence in Star Trek until First Contact, and it’s just a pretty typical but effective little scene to inform the audience of Picard’s mindset and a little bit of backstory that you might need to understand the movie. Is it the most original way to convey this information? No, but it’s not a terrible offense either and it does its job well.

“Distant Voices”

One of our least favorite episodes of all of Deep Space Nine was all just a ridiculous coma dream in Bashir’s head. If this one isn’t the worst dream episode of Trek, I don’t know what is. Like “Phantasms,” it’s got every element of the dream symbolizing something and some very tiring tropey motifs. And I haven’t even gotten to the confounding acting decisions!

“Hard Time”

While Picard got to experience a life’s worth of touching memories in “The Inner Light,” O’Brien gets to experience a life’s worth of suffering in “Hard Time.” It may have only been a couple of hours in real time, but the dream that he sustains has huge impacts on his character and makes for a really thought-provoking and riveting episode.

“Body Parts”

When he’s destined to die to relinquish his desiccated remains to Brunt, Quark at one point has one of those trauma dreams that is a writer’s shorthand for “this is what my character is preoccupied with!” In this case, he dreams of the first Nagus Gint advising him to break the contract because someone needed to propel the plot forward, I guess.

“Business as Usual” 

If “Body Parts” was a little obvious, then the dream in “Business as Usual” is downright heavy handed. Quark is feeling guilty about selling weapons on the black market, and if you weren’t sure he had a conscience, this dream sequence underlines it for you. Normally, I’d poopoo a scene like this, but O’Brien yelling “You killed my baby!” really sells it.

“Far Beyond the Stars” 

Coma dream or prophet vision? I don’t feel like arguing for the Benny Russell  story coming from some supernatural meddling versus from Sisko’s dreams, and it’s such a good episode that it’s worth bringing up anyway. And if we’re counting external meddling like in “The Inner Light” and “Hard Time,” then who am I not to include another really great episode that’s all in your head?

“Extreme Measures”

Similarly, I guess if I include “Dark Page” and “Distant Voices,” then I have to include “Extreme Measures,” though in a perfect world, I wouldn’t give it the satisfaction because it’s a really upsetting and invasive episode that turns Bashir into some kind of brain-robbing monster. And frankly, the inside of Sloan’s mind didn’t even end up being all that interesting!

“Jetrel”

Update 1/28/24: Oh no, we forgot one (or two, or probably more because dammit)! And it’s yet another of our favorite “this character is feeling conflicted about X” plot devices because writers think the audience is incapable of picking up on subtext! This time, Neelix’s history is dredged back up when Jetrel, the creator of the Metreon Cascade” pops by, and for some reason we need to see that Neelix is still having survivor’s guilt through traumatic dream vision.

“The Thaw”

Lotta coma dreams on this list so far, and our first taste from Voyager is the stuff of nightmares, literally. Fear the Clown takes what should be a paradisiacal shared dream for our friends in their cryopods and turns it into a horror movie and you could wake up dead. This one gets points for creativity, and points off for giving us all coulrophobia.

“Remember”

We really liked this one when we covered it in season three, even if it’s some more familiar ground. Reliving another culture through implanted dreams? Sure, it’s yet another instance where we can make parallels to something like “The Inner Light,” but it’s forgivable since this episode is so successful at communicating the Enarans’ story in Torres’s dreams.

“Coda”

I’d probably qualify whatever is going on with Janeway throughout this episode as something like the coma dreams we’ve discussed previously. It’s all alien interference, as happens sometimes, and winds up being like one of those episodes that pulls back the curtain and tricks you because it was fake all along, leaving the viewer ultimately unsatisfied.

“Waking Moments”

More alien intervention, this time into a world where the new species lives and communicates through dreams. Suspend your disbelief for a moment and accept that, difficult as it may be, and we actually do get some pretty original and believable dream sequences. The ones we see don’t try to go too far and mainly seem kinda right for the characters, so this one passes.

“The Fight”

But this one just fails utterly. We discussed in our recent season wrap episode how “The Fight” just seems like a chaotic mess and yet at the same time doesn’t capitalize on the Chaotic Space aliens in a way that is fulfilling. So we’re left with dream sequences that end up just feeling tired and confusing and maybe a little bit boring. Someone bet on the wrong fighter in this ring.

“One” 

While most of what Seven experiences in “One” is all delusion and hallucination, she does have a legitimate dream of being in an Arctic wasteland at one point. And sure, it’s there to convey that she’s feeling alone and desolate and way out of her depth like a pretty standard dream metaphor, but the little sequence does its job. The CGI on the other hand…

“Barge of the Dead”

Like with “Coda,” I’d qualify all of this episode as a coma dream, though instead of being induced by aliens, we’re led to believe that all of this is just some Klingon afterlife nonsense. Setting aside that everyone assumes Gre’thor is real now, the dreams we get of Torres’s personal hell – just people on the Voyager? – are half baked and entirely out of character.

“Tinker, Tenor, Doctor, Spy”

These are more daydreams than subconscious dreams, though the EMH’s program does get out of hand enough that I’d count this episode among the others in this post. The scenes we get are mostly meant to be comic relief so we can giggle at the EMH’s grandiose fantasies, except that some of them are fairly cringey, as usual at Seven’s expense.

“Memorial”

Memory or dream? That’s the question this episode poses in creating an actually pretty intriguing look at how and why we remember history. The dream sequences we get from Paris and Chakotay are very effective at blurring that line between the real and the surreal, and the more we understand that these were real events, the more we treat them with significance.

“Ashes to Ashes”

Update 1/28/24: Whoops, another I’d forgotten until we continued our Voyager watchthrough. It’s a small one, but Lyndsay Ballard’s dream sequence when she returns from the dead and back to the Voyager crew fit the old “show the audience the character is feeling conflicted” trope. She guilt-dreams that her friends are throwing her another funeral because she’s not acclimating back to life as a human. The scene does what it’s made to; it’s just entirely redundant.

“Shuttlepod One”

Let’s move on to Enterprise, starting with a pretty mean trick that dream sequences can do to an audience: manipulating you for a couple minutes into thinking that our heroes have been rescued and everything’s okay, until the rug gets pulled out from under you and it was all just a dream. The scene also leans on another trend we’ll see in Enterprise – objectifying T’Pol all the damn time.

“Fusion”

But the winner of the “objectifying T’Pol all the damn time” award is very obviously “Fusion.” Boy, what an upsetting episode. If you thought it was gross what Jev did to Troi and the others in “Violations,” then “Fusion” should have you gagging, and it all starts with a super gross dream sequence in which Tolaris creeps on T’Pol, and things get ickier from there.

“A Night in Sickbay”

Update 1/28/24: How could we forget yet another time that one of the characters had a wetdream about T’Pol? This time, it’s Archer because Phlox has put it in the cap’n’s head that he’s actually in love with the subcommander like a creep. The nightmare sequence also features another guilt-ridden message (a dream staple!) in which Archer is feeling so bad Porthos is sick that he dreams up a puppy funeral. Ugh.

“Vanishing Point”

Just when you think you’ve watched an interesting transporter accident episode that actually gives Hoshi something to do, this episode laughs in your face and pulls the “it was a dream the whole time” trick on you. The whole thing comes across as just kind of cruel and cop-out-y, though I’m kinda shocked there aren’t many other Trek episodes that consciously pull this stunt.

“Remembrance” 

Say what you will about Picard season one (and we did!), but the dream sequences of Jean-Luc obsessing over the loss of Data are very effective at eliciting an emotional response. They’re just strange enough to provide that surreal quality of dreams but not just tedious nonsense like other episodes have given us. And the scenes are just so touching that it’s hard to fault them.

“The Impossible Box”

Okay, last one I could find. Soji has these recurring trauma-fueled dreams and she can’t tell if it’s a dream or a memory. This whole episode is an identity crisis waiting to happen, and when she sees herself as a wooden puppet being constructed, it all comes together that she isn’t a real girl, and you know what, it’s both one of the better uses of dreams and one of the better moments in Picard!

—

Wake up! You’re going to be late for school and you didn’t study for that test and you’re naked and your teeth are falling out and Troi’s a cake and won’t somebody answer the phone?! 

Put a pot of coffee on and stay with us for more dreamy blogposts. You can also share that caffeinated brew with our fearless Voyager captain as we continue through our full watchthrough on SoundCloud or wherever you listen to podcasts, respond to our wake up call on Facebook and Twitter, and have pleasant dreams!