The 2DMV for Twilight Light contains various references to previous 25-ji, Nightcord de. media, as well as lots of imagery connecting to the members.
To begin with, each member is associated with an object or two that which appears frequently in the background of the 2DMV.
"of the box that gently resounds"
Kanade's item is a music box, a nod to the music box her father made for her mother. This music box played a significant role in Kanade's first unit event, Carnation Recollection, helping her to realise why her songs weren't reaching Mafuyu.
"of the sealed up cocoon"
Mafuyu's items are a birdcage and chains. This is likely a reference to the marionette doll from Mafuyu's first event, Captive Marionette, that was kept in a similar-looking cage. This doll was what started Mafuyu's arc of rediscovering her true self and realising her mother was hurting her, which culmintated in N25's second arc ender.
The lyric that accompanies this is likely a reference to Kanade's trained Determination Ignited 4* card, which contains a purple butterfly (representing Mafuyu) locked in a glass lantern.
"Inside of the picture frame that keeps chasing"
Ena's items are a picture frame and a tin of paint. The tin of paint is likely a nod to the Unsatisfied Pale Color event and Ena's trained I Don't Want to Give Up 4* card. The picture frame is a reference to the Portray Yourself set that accompanied Ena's 3rd unit event, Someday, This Wish Will Transcend the Morning Sky.
"Inside of the abandoned mirror"
Mizuki's items are a mirror and ribbons. This is a reference to the IDSMILE 2DMV, the 2DMV for Mizuki's first unit event. Both that song and Twilight Light were produced by toa.
Because of the shared producer, there are also some similar lyrics in both songs, such as "The boundaries have begun to blur, the future uncertain" from IDSMILE, and "The boundary lines where disharmonies resound with each other" from Twilight Light.
There are also a few references to N25's second arc ender, Saying Goodbye to My Masked Self, most notably there being a glitch effect when Miku sings the lines "I still can't hear you, not yet" and "I still can't see you, not yet". When she appears on a phone screen in the second chorus during the line "I'm right here", she also appears glitched. In the event, Mafuyu's phone was damaged, causing the Virtual Singers to appear glitched when their holograms appeared. Miku couldn't contact Mafuyu for a while before the phone was able to turn on again, hence the lyrics.
Miku projecting out of the phone is also a nod to how she usually manifests herself to Mafuyu.
These images appear over the following lyrics:
The melodies that we create,
The words that we pull in,
The colors that intertwine together,
And the scenes that are stitched together,
Once again they contain the items associated with the members in this MV, but the lyrics and on-screen text reference the members' respective roles within N25: composer, lyricist, artist, and editor.
Before the final chorus, the members of N25 are shown with picture frames behind them, another reference to the Portray Yourself set.
This part of the song contains multiple other references too, including:
The heart-shaped apple gem that originated as a charm on the costumes from the Snow White in Oblivion set, and later appeared as it does in this MV in some of the cards from the Where is ♡? set. Ena's card from the latter set also contains the birdcage associated with Mafuyu in this MV.
The apple is significant to Mafuyu as it was the food her mother would feed her when she was sick as a child. It was this care her mother showed her that caused her to want to become a nurse, the very dream her mother shattered. Those apples might also be Mafuyu's favorite food.
The blue butterflies that appear during this part and also at the start of the final chorus, which are a reference to the Samsa single jacket, and the Poisonous Fangs and Peeking Shadows set which had butterflies as a motif, particularly noticeable in Kanade's Determination Ignited 4* shown earlier in this post.
The phone underwater, which is actually a reference to three things. Firstly, this is a direct callback to the in-game jacket for Lower, Mizuki's second event commission.
However the phone falling in the water also links to a plot point from Saying Goodbye to My Maked Self, when Mafuyu's phone fell in her fishtank during a fight with her mother.
The throbber (loading wheel) on the screen is possibly a reference to the arc ender animation that plays after chapter 8 of the aforementioned event, which can be further backed up by the "Chasing your light, at 25:00" text that appears during that animation appearing in the 2DMV about 15 seconds after the phone is shown.
This part of the MV being underwater also connects to the Searching for a Reflection Beneath the Waters event, which introduced a lake to the Empty SEKAI.
The Heart in Water set from that event takes place underwater because of the new location, which in turn might be a nod to Mafuyu's trained Dive Into Me 4* fes card.
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