CONTAINS SPOILERS FOR GLOBAL SERVER (CARDS AND STORY CONCERNING NIGHTCORD AT 25:00)
the new cards of the new event "Immiscible Discord" in the japanese server show some interesting details in links with the progress of the story and the developement of the characters of Nightcord at 25:00 :
for Kanade's card, a 4 stars, shows us Kanade confronting Mafuyu's mom in her untrained side, cause in the summary of the story event : Mafuyu's mom is becoming more and more controlling and manipulative toward Mafuyu, who can't already take it anymore. And that constant surveillance of her mom is also affecting the group.
In her trained side, shows us a protective Kanade, with a passive agressive expression showing her interior feeling who's anger, she's basically pissed off. Also, if we look closely, Kanade is holding a cage in her arms, and in this cage there's a butterfly, strongly bright and purple and so representing Mafuyu, a fragile human being who can't stand for herself.
In the trained side of her 4 stars card : Mizuki is wearing in what's like a mischevious expression, of a person who want's nothing but the misery of others around her. That card can be interpreted in Mafuyu's mom POV : seeing Mizuki and so other members of the group being bad influence to Mafuyu, stealing her from her mom, and not seeing her anymore when Mizuki will close the flowery gate.
For Ena, we can interpret that she is also some bad influence for Mafuyu in her mom's POV. A bad influence toward her work, thinking that Ena will tell Mafuyu that she doesn't have to be a doctor, that she doesn't have to study, that studies are useless.
In the untrained side of his 3 stars card, Kaito is enraged, showing a dark aura around him, empty eyed side of him. Kaito might represent pent up rage of Mafuyu, being tired from being looked by her mom, tired to be controlled. The title of this card is "Manifestation of Fury" proving then that Kaito's presence in the empty sekai is explained by the hidden feeling of anger of Mafuyu, who's also touching Kanade, feeling her anger too. Vocaloids and Sekais are the representations of feeling of people, and Kaito is the "humanification" of Mafuyu's anger. Plus, at one moment of the event story (i don't remember wich chapter precisely, sorry), there's a scene of a discussion between Kaito and Mafuyu, where they're arguing about how her situation with her mom got worse, and Kaito's wearing the expression of anger, talking hard to Mafuyu, and so showing his boiling rage who's hers too.
Here's my interpretation (and maybe most of the fandom) about the new set and event "Immiscible Discord" in the jp server of Project Sekai. Thank you for reading! And also don't hesitate to give your opinion and personnal interpretation of the cards! ^_^