#Box Expert

[boxexpert]

Inspirasi Kotak Produk Kecantikan Dari Negara Portugal: Pelajaran Keusahawanan dari Benamor

Dalam arena kecantikan global yang sentiasa berkembang, pembungkusan produk telah menjadi elemen kritikal dalam strategi pemasaran. Kini, kita akan meneroka sumber inspirasi yang mungkin kurang dikenali tetapi sangat berpotensi – Portugal. Negara ini bukan sahaja terkenal dengan warisan budayanya yang kaya, tetapi juga sedang muncul sebagai peneraju dalam inovasi pembungkusan produk…

[mysticdragon3md3]

[gummi-ships]

Kingdom Hearts 3 - The Caribbean

[liass-21]

Lia! Give me some advice on how to write Ethan as babygirl as possible —AssignmentImprobable

hi stevie beautiful. the only advice i have is to make him slightly pathetic at all times (as it is written in the holy canon). additionally he should always be 15% concerned that everyone hates him. additionally he should always be injured either mentally or physically or both (both is ideal). additionally he should be naked. additionally he should be desperate for postive affirmation but simultaneously incredibly averse to asking for it. additionally he should believe at a molecular level that he didn't do enough no matter what the task at hand is. additionally he should maintain an aura of recently divorced even if mi3 was 5 movies ago. additionally he should be naked.

[atopvisenyashill]

au where roose and cat start hooking up after ned dies and roose catches what you might call feelings and triple agents for the starks (for cat)

[glitteraffe-art]

This is a pretty simple explanation of the topic, and does not cover every detail, but hopefully it is a nice overview!

Slide text in text form, and reference and image source URLs under the cut:

Title Card: Cloning Mercenaries 101: How Real-Life Cloning Works

CW: Mentions of animal death (in a research context)

Slide 1: Foreword

If you're into TF2, chances are you've come across fanworks depicting the "clone theory" to explain why there's more than one of each mercenary.

Clone theories generally fall into the categories of either respawn/teleportation cloning, or biological cloning. Today, we'll focus on real-life biological cloning.

"But TF2 is a Looney-Tunes comedy! Why bother with the science of a fairly common, but minimally supported headcanon?"

It's always worth understanding how the real-life version of something really works!

Slide 2: What is Cloning?

"Cloning" broadly refers to the process of producing individuals who are genetically identical to another individual. It can include single- and multi-cellular organisms.

Cloning includes both natural cloning (like plant cuttings, asexual reproduction) and artificial cloning (like cloning DNA fragments, cells, and organisms).

"Reproductive cloning", is the cloning of a multicellular organism to create a new genetically identical individual.

Engie speech bubble: Natural cloning is more common than you may think: for example, plants grown from cuttings are clones of their "parent"!

Slide 3: Reproductive Cloning

There are 2 methods of reproductive cloning for mammals:

Embryo Splitting

- Used to create identical twins from 1 egg and 1 sperm cell after in-vitro fertilization (IVF)

- Not really for cloning an existing adult so we won't focus on it.

Somatic Cell Nuclear Transfer (SCNT)

- Used to create a number of clones of an individual organism from 1 body cell (somatic cell) from the organism and 1 donor egg cell

Engie speech bubble: We’ll be focusing on SCNT here!

Slide 4: Somatic Cell Nuclear Transfer (Diagram)

1. Nucleus removed from body (somatic) cell of individual to be cloned (by pipette)

2. Nucleus removed from donor egg cell

3. Somatic nucleus placed into empty donor egg cell

4. Resulting egg stimulated and begins to divide

5. Resulting embryo transferred to uterus of a surrogate mother to develop.

Slide 5: Somatic Cell Nuclear Transfer

The resulting clone embryo is not 100% identical to that of the individual to be cloned.

- The body (somatic) cell's nucleus may have contained mutations

- The mitochondria (powerhouse of the cell) of the resulting clone embryo will be the donor egg's mitochondria

That's right! If the donor egg is NOT from the individual or their matenal line (mitochondria are passed down maternally) the resulting clone embryo will NOT be 100% identical.

Engie speech bubble: With different mitochondria, a clone may have differences in body parts with high energy demand, like muscles, heart, eyes, or brain.

Slide 6: Dolly the Sheep

Some methods using the principles of SCNT were used as early as the 1950s (based on earlier work in the 1920s) to clone amphibians.

The first successful mammalian clone using SCNT was Dolly, a cloned sheep born in 1996 in Scotland, and announced to the public in 1997.

Dolly eventually developed severe arthritis and a progressive lung disease and was

euthanized at age 6 despite sheep's average lifespan being 11-12 years.

Engie speech bubble: Dolly was named after Dolly Parton!

Slide 7: Some Downsides

Inefficiency & Failure Rate:

SCNT has a high failure rate - many clones die during gestation, and newborn clones may die of abnormalities. For example, for Dolly, 277 fertilized eggs -> 29 embryos -> 3 lambs born -> only Dolly survived.

Advanced Aging(?):

Due to Dolly's age-related diseases it was speculated her DNA was already genetically "old". Later studies of further clones found no evidence of having age-related diseases, but the idea of "advanced" or "accelerated" aging of clones made its way into pop culture.

Engie speech bubble: More recent cloning using SCNT have reported higher success rates, but still have many failures.

Slide 8: What Does this Mean in TF2?

A resulting baby born from SCNT will, in fact, be a baby. The baby will NOT have the memories, experiences, muscle mass, or, well... anything past genetics, of the individual they were cloned from.

In terms of TF2, a lot of time and money would need to be spent raising, feeding, and teaching the resulting baby for the next 18 or so years to get anywhere close to a useful mercenary. Not very efficient...

Engie speech bubble: In short, you'd need to look beyond realistic cloning

and take some sci-fi shortcuts to get already-adult clones as often portrayed in fandom.

REFERENCE URLs

(Removed the hyper links since Tumblr likes to hide posts with hyperlinks)

Image of Dolly is from here:

http://news.bbc.co.uk/onthisday/hi/dates/stories/february/22/newsid_4245000/4245877.stm

I mainly referenced these while writing (Yes, Wikipedia unreliable, I know, but this slideshow is a for-funsies TF2-themed general explanation and not an academic essay):

https://www.ncbi.nlm.nih.gov/books/NBK223960/

https://en.m.wikipedia.org/wiki/Dolly_(sheep)

https://en.m.wikipedia.org/wiki/Somatic_cell_nuclear_transfer

https://en.m.wikipedia.org/wiki/Cloning

I also looked at these when trying to determine if the Wikipedia information was reliable:

https://www.ncbi.nlm.nih.gov/books/NBK223969/

https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/General_Biology_(Boundless)/17%3A_Biotechnology_and_Genomics/17.01%3A_Biotechnology/17.1D%3A_Reproductive_Cloning

https://www.britannica.com/science/cloning/Reproductive-cloning

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790123/

[luminacerin]

No because you don’t understand — Xie Lian would be SHIT at any kind of game that isn’t candy crush EXCEPT Beat Saber.

Hua Cheng would get him a VR set and Xie Lian would go absolutely WILD.

[telekitnetic-art]

Are there limitations on who's allowed to learn and draw formline art? Because it's always been a style I wanted to learn growing up but I'm not sure if it's a style from a different tribe that has rules on it.

So, technically formline art does not “belong”, per se, to one tribe. The title of formline is more of an umbrella term for the style of artwork that is common with PNW-based nations and tribes. Most artists I see either label themselves as formline artists or either use the name of their nation as the descriptor for their art.

Legally and culturally, most formline artists I’ve spoken to are open to the idea of people learning formline art, native or non-native. It’s jumping into making a profit off of formline art right away that is generally very frowned upon, especially with non-native people. Cultural appropriation is definitely looked on unfavourably with a lot of Indigenous artists and creators. Depending on your location and nation/tribe, some community centres or nations offer classes/courses revolving around traditional art and formline art, and I recommend checking out those resources if you can. The website Sealaska Heritage also has plenty of resources as well as video tutorials and it could be possible to find classes there as well.

Hope that helps!

[hotwaterandmilk]

Hi! I was wondering if you have any experience scanning books? I have several manga art books that have yet to be scanned by anyone and uploaded online and so I'd love to do it, but I'm really worried about damaging the books' spines. I was wondering if you have any tips?

Hi, I post quite a few art book scans on here so I definitely understand where you're coming from. While it is feasible to scan without damaging the spine too badly, realistically you will always end up inflicting some stress to it when scanning. Whether this ends up being enough to really damage or split the spine will really depend on the pressure involved and the condition of the spine.

I think QuicksandScans has a solid guide to scanning books and I tend to agree with them that debinding a book (and thus destroying it) is the only way to avoid any form of gutter shadow or page warp. However, this is only an option you can really choose if you have a duplicate copy of a book for the explicit purpose of debinding imho.

In most cases I don't have a duplicate copy so I tend to compromise and only scan the images that won't completely destroy a book if I apply heavy pressure to it. There are also scanning rig options where people essentially use their phones or other devices to scan via a makeshift rig so that a book can be open but not under pressure while being scanned (thus minimising damage), but I haven't got the space to try this myself so can't vouch for how effective it is in terms of preserving quality.

At the end of the day all scanning does have this inherent risk to it in that we could end up damaging our physical items in an attempt to preserve their content. I tend not to take that risk if I feel an item is too fragile, but as you get more confident with your scanning I think you'll come to know your scanner and your items well enough to know when you're pushing their limits.

Good luck, I hope you can scan your books in a way that works for you <3

[pianokantzart]

I forgot to take my ADHD medication for two days and decided to take up carpentry, and now I'm in too deep to back out.

[boxexpert]

Estée Lauder Companies: Legenda Kecantikan Dunia & Rahsia Kotak Produk yang Menawan

Bila kita sebut nama Estée Lauder, boleh jadi ramai yang terus terbayang serum ikonik dalam botol kaca coklat, gincu-gincu mewah yang tampak eksklusif, atau bauan wangi dari jenama-jenama elit seperti Jo Malone dan Tom Ford. Namun ada satu rahsia penting di sebalik kejayaan global mereka yang jarang dibincangkan:Kotak produk yang memikat dan dirancang dengan penuh strategi. Dalam dunia yang…

[hitwiththefandomz]

The In This Together boys are expert scavengers. Need any tools or supplies for your crafts? They can find it. Open to trading and/or working for food.

(Cabin 15, Fire Nation!)

10/10 service

@tmnt-fandom-family-reunion

Cabin #7 (7 Wonders of the Turtleverse)

[unopenablebox]

you can knit directly from a mawata silk handkerchief right

do you have to know how to spin particularly to do it

[skeksismars]

The real representation in Dungeon Menshi is Laios being Otherkin

[theanticool]

Watch Women’s Boxing

[cornertheculprit]

the thing about kazuma asogi and barok van zieks is that they're both swordsmen but you know who's ALSO a swordsman as per the canon he's adapted from? herlock sholmes