#Blood glucose

[glucose-gay]

my wii fit arrived yesterday afternoon and i immediately went too hard

[studychemistrytopics]

Glucose Level in the Blood, normal blood sugar levels in the human body with health benefits for more about glucose molecule

[fatgirlgetsfitatlast]

2/24/24 PlyoJam online, 2nd PlyoJam of the day, 3rd workout of the day

I am done. Had to phone in a good bit of this, walk through the moves. My legs are dead.

[jcmarchi]

Noninvasive Technique Reveals How Cells’ Gene Expression Changes Over Time - Technology Org

New Post has been published on https://thedigitalinsider.com/noninvasive-technique-reveals-how-cells-gene-expression-changes-over-time-technology-org/

Noninvasive Technique Reveals How Cells’ Gene Expression Changes Over Time - Technology Org

MIT researchers can now track a cell’s RNA expression to investigate long-term processes like cancer progression or embryonic development.

DNA – artistic impression. Image credit: Image by kjpargeter on Freepik

Sequencing all of the RNA in a cell can reveal a great deal of information about its function and what it is doing at a given time. However, the sequencing process destroys the cell, making it difficult to study ongoing changes in gene expression.

An alternative approach developed at MIT could enable researchers to track such changes over extended periods of time. The new method, which is based on a noninvasive imaging technique known as Raman spectroscopy, doesn’t harm cells and can be performed repeatedly.

Using this technique, the researchers showed that they could monitor embryonic stem cells as they differentiated into several other cell types over several days. This technique could enable studies of long-term cellular processes such as cancer progression or embryonic development, and one day might be used for diagnostics for cancer and other diseases.

“With Raman imaging you can measure many more time points, which may be important for studying cancer biology, developmental biology, and a number of degenerative diseases,” says Peter So, a professor of biological and mechanical engineering at MIT, director of MIT’s Laser Biomedical Research Center, and one of the authors of the paper.

Koseki Kobayashi-Kirschvink, a postdoc at MIT and the Broad Institute of Harvard and MIT, is the lead author of the study, which appears today in Nature Biotechnology. The paper’s senior authors are Tommaso Biancalani, a former Broad Institute scientist; Jian Shu, an assistant professor at Harvard Medical School and an associate member of the Broad Institute; and Aviv Regev, executive vice president at Genentech Research and Early Development, who is on leave from faculty positions at the Broad Institute and MIT’s Department of Biology.

Imaging gene expression

Raman spectroscopy is a noninvasive technique that reveals the chemical composition of tissues or cells by shining near-infrared or visible light on them. MIT’s Laser Biomedical Research Center has been working on biomedical Raman spectroscopy since 1985, and recently, So and others in the center have developed Raman spectroscopy-based techniques that could be used to diagnose breast cancer or measure blood glucose.

However, Raman spectroscopy on its own is not sensitive enough to detect signals as small as changes in the levels of individual RNA molecules. To measure RNA levels, scientists typically use a technique called single-cell RNA sequencing, which can reveal the genes that are active within different types of cells in a tissue sample.

In this project, the MIT team sought to combine the advantages of single-cell RNA sequencing and Raman spectroscopy by training a computational model to translate Raman signals into RNA expression states.

“RNA sequencing gives you extremely detailed information, but it’s destructive. Raman is noninvasive, but it doesn’t tell you anything about RNA. So, the idea of this project was to use machine learning to combine the strength of both modalities, thereby allowing you to understand the dynamics of gene expression profiles at the single cell level over time,” Kobayashi-Kirschvink says.

To generate data to train their model, the researchers treated mouse fibroblast cells, a type of skin cell, with factors that reprogram the cells to become pluripotent stem cells. During this process, cells can also transition into several other cell types, including neural and epithelial cells.

Using Raman spectroscopy, the researchers imaged the cells at 36 time points over 18 days as they differentiated. After each image was taken, the researchers analyzed each cell using single molecule fluorescence in situ hybridization (smFISH), which can be used to visualize specific RNA molecules within a cell. In this case, they looked for RNA molecules encoding nine different genes whose expression patterns vary between cell types.

This smFISH data can then act as a link between Raman imaging data and single-cell RNA sequencing data. To make that link, the researchers first trained a deep-learning model to predict the expression of those nine genes based on the Raman images obtained from those cells.

Then, they used a computational program called Tangram, previously developed at the Broad Institute, to link the smFISH gene expression patterns with entire genome profiles that they had obtained by performing single-cell RNA sequencing on the sample cells.

The researchers then combined those two computational models into one that they call Raman2RNA, which can predict individual cells’ entire genomic profiles based on Raman images of the cells.

Tracking cell differentiation

The researchers tested their Raman2RNA algorithm by tracking mouse embryonic stem cells as they differentiated into different cell types. They took Raman images of the cells four times a day for three days, and used their computational model to predict the corresponding RNA expression profiles of each cell, which they confirmed by comparing it to RNA sequencing measurements.

Using this approach, the researchers were able to observe the transitions that occurred in individual cells as they differentiated from embryonic stem cells into more mature cell types. They also showed that they could track the genomic changes that occur as mouse fibroblasts are reprogrammed into induced pluripotent stem cells, over a two-week period.

“It’s a demonstration that optical imaging gives additional information that allows you to directly track the lineage of the cells and the evolution of their transcription,” So says.

The researchers now plan to use this technique to study other types of cell populations that change over time, such as aging cells and cancerous cells. They are now working with cells grown in a lab dish, but in the future, they hope this approach could be developed as a potential diagnostic for use in patients.

“One of the biggest advantages of Raman is that it’s a label-free method. It’s a long way off, but there is potential for the human translation, which could not be done using the existing invasive techniques for measuring genomic profiles,” says Jeon Woong Kang, an MIT research scientist who is also an author of the study.

Written by Anne Trafton

Source: Massachusetts Institute of Technology

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[the-barrel-man]

I was taking my blood glucose reading and when I pricked myself the droplet came out in a shape of a heart ♥️ what does it mean?

[suzieb-fit]

Yeah, this is my diabetes at the moment.

The green band is my target range. And this is one of the reasons I can't get myself into my usual focus on fasting, diet or workouts.

Some days I'm on it, some days I'm waaaaay off it!

I'm now at the full post menopause stage. Just ended my 12 months without a period.

Shouldn't I get, like, a certificate or something? 🤔😂.

I'm just on the threshold of moving house. That's been a major stressor for at least the last couple of months.

Insomnia is also off the scale.

Keep trying different fasting schedules. the FLAG book programme, the lunar cycle system and the very recent (and extremely short lived) sardine thing. I'm clearly all over the place in quite a few different ways!

Need to get moved, get my head straight, get my sugar level control and sleep routine running a bit more smoothly and get ME back!

I know she's still in there, waiting to get back out 😋

[sp00kysk3lly]

I’m in the middle of doing my #millionstepsfordiabetesuk

I wondered if anyone could be kind enough to donate to a great cause, that has helped me and so many other people.

I was diagnosed with Type One Diabetes in 2016 when I was 18 years old.

Since then I have also been diagnosed with EPI (Exocrine Pancreatic Insufficiency), Diabetic Retinopathy, and Chronic Pain. I have also lost my teeth due to bad control and being unsupported by my diabetes team.

Even if it is only a few pence, or a few pounds (even if you’re in America or other countries), every penny helps!

All money goes to DiabetesUK to help other diabetics get the help they need!

This is a really important cause for me. I know I have only done 254,131 steps but I am dealing with chronic pain and I’m logging every step I am taking even if it isn’t much.

These guys have helped me with so much. Even if I didn’t know it yet.

Without donations I probably wouldn’t have got the pump or sensor that have helped me lower my HbA1c or my blood sugar!

Please help where and when you can. This challenge finishes on 30/09/23! 30th September 2023!

Please help me get more money for this amazing charity and the amazing people that have helped me and others so much!

[diabeticfooddiary]

12:25pm august 23, 2022

172mg/dl

[new-health-products-in]

Glucofreeze Supplements

Taking Control of My Blood Sugar with Glucofreeze Supplements

For years, I'd been struggling with maintaining healthy blood sugar levels. It felt like a constant battle, and despite dietary changes, exercise, and even some medication, I wasn't seeing the results I craved. That's when I decided to try Glucofreeze Supplements.

A Natural Approach to Blood Sugar Support

Glucofreeze appealed to me because it's a natural supplement. The formula contains ingredients like cinnamon, turmeric, and berberine, all of which have been shown to support healthy blood sugar metabolism. I was also impressed that it's manufactured in the USA and adheres to strict quality control standards.

Feeling the Positive Effects

Since incorporating Glucofreeze into my daily routine, I've noticed a positive difference. My blood sugar readings have become more stable, and I feel a renewed sense of energy throughout the day. I no longer experience those afternoon slumps that used to plague me. Plus, I sleep much better at night, which I'm sure contributes to my overall well-being.

A Valuable Addition to My Health Regimen

Glucofreeze hasn't been a miracle cure, but it's definitely become a valuable addition to my health regimen. It's helped me manage my blood sugar levels more effectively, and I feel fantastic for it. If you're looking for a natural way to support your blood sugar health, I highly recommend giving Glucofreeze a try.

Disclaimer:It's important to note that I'm not a medical professional, and these are just my personal experiences. It's always best to consult with your doctor before starting any new supplements, especially if you have any underlying health conditions.

[drforambhuta]

The Significance of Comprehensive Health Checkups in Peritoneal Dialysis:

Regular full-body health evaluations are essential for maximizing outcomes and improving long-term survival among individuals undergoing peritoneal dialysis (PD). These assessments encompass a range of diagnostic techniques aimed at evaluating various health aspects, including kidney function, cardiovascular health, nutritional status, and metabolic indicators. Key elements of these evaluations for PD patients include:

1. Kidney Function Assessment: Routine monitoring of parameters such as serum creatinine, blood urea nitrogen (BUN), and estimated glomerular filtration rate (eGFR) aids in the early detection of declining kidney function. This information guides decisions on dialysis adequacy and modality selection.

2. Cardiovascular Screening: Thorough cardiovascular evaluations, including electrocardiography (ECG), echocardiography, and cardiac biomarker tests, provide insights into cardiac health and function. Early identification and management of cardiovascular risk factors like hypertension, dyslipidemia, and coronary artery disease are crucial for preventing cardiovascular complications and improving survival rates among PD patients.

3. Nutritional Status Assessment: Monitoring nutritional status through biochemical markers (e.g., serum albumin, prealbumin, and total protein), anthropometric measurements, and dietary assessments helps in early detection of malnutrition. This information guides interventions aimed at optimizing protein-energy status and preserving lean body mass in PD patients.

4. Metabolic Evaluation: Assessing metabolic parameters such as serum electrolytes, glucose, lipid profile, and markers of mineral and bone metabolism (e.g., calcium, phosphorus, and parathyroid hormone) assists in detecting and managing metabolic imbalances common in PD patients. Optimizing these parameters is crucial for reducing the risk of metabolic bone disease, cardiovascular calcification, and other metabolic complications associated with end-stage renal disease (ESRD).

There are several good hospitals in India that offer health checkup packages to choose from based on an individual's health status and requirements. A regular full body health checkup helps in increasing the survival rate of patients undergoing peritoneal dialysis.

[agnihothealthforall]

Amiclear: Don't Purchase Before You Have Read This True Opinion About It! Looking for a natural method to maintain steady blood sugar levels? Most patients fear, "Well, if I actually don't burn calories or if my blood sugar refuses to optimise," which is a distinct possibility. This is a reasonable issue, considering that many diabetes patients have already tried all therapeutic choices without result. In this sense, AmiClear stands out in particular.

As a consequence, thousands of people have been able to lose weight and bring their blood sugar under control. AmiClear is an all-natural solution that employs a mix of vitamins and herbs to assist the body in regulating glucose levels.

AmiClear's contents were selected based on their history of use in traditional medicine to benefit health, and especially to assist maintain normal blood sugar levels. Additionally, it has several other advantages, So please be patient while we publish the multiple stories we have prepared for you….

[fatgirlgetsfitatlast]

2/29/24 : 2 mile walk, morning

I'm trying to remember to log on the CGM the start and end of exercise sessions/walks to make it easier to see the effects. Today's was pretty clear: even a moderate walk brings it down from the overnight elevation.

[healthysharpreviews]

Have you wondered what could cause your blood sugar level to rise abnormally? One major risk factor? Being overweight or obese. Here comes GlucoTrust, a natural supplement introduced to help control high blood sugar. Detailed review on how GlucoTrust works here:

re: https://healthysharpreviews.com/glucotrustingredients

[medugo]

How to Record Your Blood Sugar Level?

With Medugo, you can record and view your blood sugar level easy and quickly. We show you step by step, how to record your sugar level reading in Medugo.

Medugo makes it easy to use the language of your choice. English, Tamil, Hindi, Malayalam, Telugu, Kannada, Odia and Bengali. 

YOUR HEALTH VITALS ON THE GO 

Download the App Now: 

Play Store: https://shorturl.at/afgxP 

App Store: https://shorturl.at/foyX2 

Website: https://www.medugo.com/

[suzieb-fit]

The latest additions to my ever growing library!

I am starting with "The Glucose Revolution"

Time to curl up in the sunshine with a decaff and start reading!

I really like that a couple of the testimonials come from fellow type one diabetics. It's always all about type two these days.

Type one has been totally drowned out.

Back in the day, if you said you were diabetic, it most usually meant type one. Type two has completely taken over.

Anyway, looking forward to digging in!